CN112759489A - Microbial organic fertilizer for relieving crop continuous cropping obstacle and preparation and application thereof - Google Patents

Microbial organic fertilizer for relieving crop continuous cropping obstacle and preparation and application thereof Download PDF

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CN112759489A
CN112759489A CN202110133029.7A CN202110133029A CN112759489A CN 112759489 A CN112759489 A CN 112759489A CN 202110133029 A CN202110133029 A CN 202110133029A CN 112759489 A CN112759489 A CN 112759489A
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organic fertilizer
microbial organic
rhodopseudomonas palustris
crops
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谢建平
谢富玲
许峻旗
代永东
海洋
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Southwest University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C9/00—Fertilisers containing urea or urea compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
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    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention belongs to the technical field of microbial organic fertilizers, and particularly discloses a microbial organic fertilizer for relieving continuous cropping obstacles of crops, and preparation and application thereof. The microbial organic fertilizer takes rhodopseudomonas palustris (CGMCC No.1.2180) as a main strain, straw, dry pig manure or cow manure as a culture medium, and also comprises urea and brown sugar. The microbial organic fertilizer can effectively degrade toxic substances secreted into soil by the allelopathy of the pepper, particularly the pepper in karst landform areas, relieve the continuous cropping obstacle of pepper planting land and provide rich nutrients. The microbial organic fertilizer is applied to pepper cultivation, so that the ecological environment of karst landform areas can be recovered by using the characteristics of the karst landform without influencing the economic benefit of people. The microbial organic fertilizer can also solve the problem of continuous cropping obstacles of other crops.

Description

Microbial organic fertilizer for relieving crop continuous cropping obstacle and preparation and application thereof
Technical Field
The invention relates to the technical field of microbial organic fertilizers, in particular to a microbial organic fertilizer for relieving continuous cropping obstacles of crops and preparation and application thereof.
Background
The continuous cropping obstacle refers to the abnormal growth and development of crops caused by continuously cultivating the same crop or closely related crops on the same soil. Continuous cropping obstacles exist in many crops, such as pepper, melon, bitter gourd, tomato, eggplant and the like.
Fructus Zanthoxyli (Zanthoxylum. L) belongs to Rutaceae (Rutaceae), deciduous shrub or small tree, its fruit contains aromatic oil, not only is important food flavoring raw material, but also can be used as antiseptic, and its fruit, rhizome and leaf can be used as medicine, and has effects of dispelling pathogenic wind and cold, promoting blood circulation and relaxing muscles and tendons, relieving pain, anesthesia, resisting bacteria, killing parasite, resisting cancer, etc.; the extract or volatile oil can resist virus, prevent and kill germs and microorganisms, and is an important economic and medicinal plant. Experimental research shows that the allelopathy of the pepper is an important cause of continuous cropping obstacle, and directly reduces the efficiency and the period of land utilization.
In areas such as Guizhou Yuanyang, the pepper is a common crop. However, as the Guizhou Yuyang area belongs to a severe ecological degradation area under the karst landform, the ecological environment is very fragile, and the ecology of the area needs to be recovered more urgently due to the soil continuous cropping obstacle caused by continuous planting of the pepper. Therefore, how to effectively relieve or reduce the continuous cropping obstacles caused by the allelopathy of the pepper in the karst landform area and how to reasonably utilize the characteristics of the local terrain without influencing the economic benefit of people to recover the ecological environment becomes a problem which needs to be solved urgently.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a microbial organic fertilizer for relieving crop continuous cropping obstacles, and preparation and application thereof, and aims to solve the continuous sitting problem of pepper planting, especially pepper planting in karst landform areas, and also solve the continuous cropping obstacles of other crops.
In order to achieve the above objects and other related objects, a first aspect of the present invention provides a microbial organic fertilizer for alleviating crop continuous cropping obstacles, comprising a strain and a culture medium, wherein the strain is rhodopseudomonas palustris, and the preservation number of the rhodopseudomonas palustris is CGMCC No. 1.2180.
Further, the culture medium comprises straw and animal manure, and the animal manure is selected from at least one of pig manure and cow manure.
Optionally, the straw is at least one selected from wheat straw, corn stalk and straw, and the pig manure and the cattle manure are dried pig manure and cattle manure.
Further, in the microbial organic fertilizer, the rhodopseudomonas palustris is rhodopseudomonas palustris liquid, and the weight of the rhodopseudomonas palustris liquid accounts for 5-20% of the weight of the microbial organic fertilizer.
Furthermore, in the microbial organic fertilizer, the use amount of the straws is equal to that of the animal wastes.
Furthermore, in the microbial organic fertilizer, the using amounts of pig manure and cow manure are equal.
Further, the microbial organic fertilizer also comprises urea and brown sugar, and the weight ratio of the culture medium to the urea to the brown sugar in the microbial organic fertilizer is 4-5: 0.1-0.3: 0.2-05.
In a second aspect of the present invention, there is provided a method for preparing a microbial organic fertilizer for alleviating crop continuous cropping obstacles, including the following steps:
mixing and stirring raw materials (straw, animal manure, urea and brown sugar) uniformly according to a weight ratio, adding the rhodopseudomonas palustris liquid, carrying out closed anaerobic fermentation on the materials after fully stirring uniformly, and standing at room temperature for 2-4 weeks after fermentation is finished, and then ripening to obtain the microbial organic fertilizer.
Furthermore, the amount of the rhodopseudomonas palustris liquid is adjusted according to the dryness of the materials, the water content of the fully and uniformly stirred materials is controlled to be 25-30 percent, preferably 25 percent, and the materials are easy to agglomerate but do not produce water when being pinched by hands.
Further, the closed anaerobic fermentation conditions are as follows: 35-40 deg.C, 36-56 hr, preferably 37 deg.C, 48 hr.
Further, the total viable count of the microbial organic fertilizer is controlled to be 1.0-5.0 hundred million/g.
Further, the culture method of the rhodopseudomonas palustris liquid comprises the following steps: inoculating Rhodopseudomonas palustris in liquid culture medium of Pseudomonas palustris, and performing amplification culture when viable count is equal to or higher than 1.0 × 109Stopping the amplification culture when the concentration is CFU/mL, and obtaining the rhodopseudomonas palustris liquid.
Optionally, the temperature during the amplification culture is 28-32 ℃.
Optionally, the pseudomonas liquid culture medium formula and the preparation method thereof are as follows: 16.0g/L of gelatin peptone and 10.0g/L, K of trypsin2SO4 10.0g/L、MgCl21.4g/L hexadecyl trimethylamine bromide 0.2g/L, final pH 7.1 + -0.2 (25 ℃), the preparation method is: taking gelatin peptone, pancreas egg, K2SO4、MgCl2Adding 1L of distilled water or deionized water into the hexadecyl trimethylamine bromide, adding 10mL of glycerol, stirring, heating and boiling until the mixture is completely dissolved, and carrying out autoclaving at the temperature of 120-.
In a third aspect of the invention, the microbial organic fertilizer according to the first aspect and/or the microbial organic fertilizer prepared by the preparation method according to the second aspect is applied to alleviating the sitting disorder of crops.
Further, the crops are selected from at least one of pepper, hot pepper, melon, bitter gourd, tomato and eggplant.
Further, the planting land of the crops is karst landform.
Further, the planting land of the crops is a land of Guiyang and/or unitary Yang.
Further, the application method of the microbial organic fertilizer comprises the following steps:
(1) application: in the previous month of crop planting, mixing the microbial organic fertilizer with soil in a hole application mode;
(2) cultivation: in the crop planting period, crops are cultivated in a conventional mode, and each crop is planted in the central position of the microbial organic fertilizer hole application;
(3) applying a bacterial solution: after the crop seedlings come out of the soil, the diluted rhodopseudomonas palustris liquid is applied to the roots for 1 time.
Optionally, the weight ratio of the microbial organic fertilizer to the soil is 5-15: 100, and the weight of the soil in each hole is 0.6-1.5 kg.
Optionally, the rhodopseudomonas palustris liquid is diluted by water by a factor of 80-120.
As mentioned above, the microbial organic fertilizer for relieving pepper continuous cropping obstacle and the preparation and application thereof have the following beneficial effects:
the invention takes rhodopseudomonas palustris (CGMCC No.1.2180) as a main strain, straws and animal manure (dry pig manure and/or dry cow manure) as culture medium, and then urea and brown sugar are added to prepare a novel microbial organic fertilizer; the hole application method is adopted to mix with the soil in the crop planting area, the mixture is cultivated for one month, and then the mixture is planted according to the conventional method, so that the crop yield can be effectively improved, harmful substances in the soil can be effectively degraded, the problem of continuous cropping obstacle can be relieved, rich nutrients can be provided for the crops, and the effect of fertilizing the soil can be achieved. In addition, the microbial organic fertilizer has simple raw material composition, and the culture medium uses agricultural wastes (straws, pig manure and cow manure) as much as possible, so the cost is low.
Experiments prove that the microbial organic fertilizer can effectively degrade toxic substances secreted into soil by the allelopathy of the pepper, particularly the pepper in karst landform areas, relieve the continuous cropping obstacle of pepper planting land and provide rich nutrients. The microbial organic fertilizer is applied to pepper cultivation, so that the ecological environment of karst landform areas can be recovered by using the characteristics of the karst landform without influencing the economic benefit of people. Certainly, the microbial organic fertilizer can also solve the problem of continuous cropping obstacles of other crops, such as hot peppers, melons, bitter gourds, tomatoes, eggplants and the like.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The invention provides a microbial organic fertilizer for relieving crop continuous cropping obstacles, which comprises a strain and a culture medium, wherein the strain is rhodopseudomonas palustris, and the preservation number of the rhodopseudomonas palustris is CGMCC No. 1.2180; the microbial organic fertilizer also comprises urea and brown sugar.
Rhodopseudomonas palustris (CGMCC No.1.2180, storage time: 1/5/1998) is a photosynthetic microorganism and is widely distributed in rivers, lakes and oceans. The rhodopseudomonas palustris has good tolerance and has degradation effect on pesticides, herbicides and other toxic chemical substances. For example, P.palustris can dechlorinate and mineralize tetrachlorophenol. Meanwhile, rhodopseudomonas palustris, an anaerobic phototrophic bacterium, has a biodegradation and metabolic effect on unusual carbon compounds (aromatic compounds and aliphatic hydrocarbons). In addition, Rhodopseudomonas palustris cells are rich in high-value biochemical substances (vitamin B, coenzyme Q10, pigments, folic acid, etc.), and are non-toxic and harmless. Therefore, the rhodopseudomonas palustris is used for preparing a microbial organic fertilizer, and is applied to the crop cultivation soil to degrade a series of harmful substances secreted by the allelopathy of crops such as pepper and the like, so that the problem of continuous cropping obstacle of the crops such as pepper and the like is solved, and the problem of continuous cropping obstacle of the crops such as pepper and the like is particularly solved in karst landform areas such as Guizhou Yang.
Specifically, the culture medium comprises straw and animal manure, wherein the animal manure is at least one of pig manure and cow manure; preferably, the straw is at least one selected from wheat straw, corn stalk and rice straw, and the pig manure and the cattle manure are dried pig manure and cattle manure.
Specifically, in the microbial organic fertilizer, the rhodopseudomonas palustris is rhodopseudomonas palustris liquid, the weight of the rhodopseudomonas palustris liquid accounts for 5-20% of the weight of the microbial organic fertilizer, and the weight ratio of a culture medium, urea and brown sugar in the microbial organic fertilizer is 4-5: 0.1-0.3: 0.2-05; wherein, in the microbial organic fertilizer, the use amount of the straws is equal to that of the animal wastes; when the animal manure is selected from pig manure and cow manure, the optimal use amount of the pig manure and the cow manure is equal.
The preparation method of the microbial organic fertilizer comprises the following steps:
mixing and stirring raw materials (straw, animal manure, urea and brown sugar) uniformly according to a weight ratio, adding the rhodopseudomonas palustris liquid, adjusting the use amount of the rhodopseudomonas palustris liquid according to the drying degree of the materials, controlling the water content of the materials to be 25-30%, properly kneading the materials into a mass easily but not yielding water, and performing closed anaerobic fermentation on the materials after fully stirring uniformly, wherein the closed anaerobic fermentation conditions are as follows: 35-40 ℃ and 36-56 h; after fermentation, the mixture is placed at room temperature for 2-4 weeks and then is matured to prepare the microbial organic fertilizer, and the total viable count of the microbial organic fertilizer is controlled to be 1.0-5.0 hundred million/g.
Specifically, the culture method of the rhodopseudomonas palustris liquid comprises the following steps: inoculating rhodopseudomonas palustris in a pseudomonas liquid culture medium, and carrying out amplification culture at the temperature of 28-32 ℃ until the viable count is equal to or higher than 1.0 multiplied by 109Stopping the amplification culture when the concentration is CFU/mL, and obtaining the rhodopseudomonas palustris liquid.
The formula of the pseudomonas liquid culture medium and the preparation method thereof are as follows: 16.0g/L of gelatin peptone and 10.0g/L, K of trypsin2SO4 10.0g/L、MgCl21.4g/L hexadecyl trimethylamine bromide 0.2g/L, final pH 7.1 + -0.2 (25 ℃), the preparation method is: taking gelatin peptone, trypsin and K2SO4、MgCl2Adding 1L of distilled water or deionized water into the hexadecyl trimethylamine bromide, adding 10mL of glycerol, stirring, heating and boiling until the mixture is completely dissolved, and carrying out autoclaving at the temperature of 120-.
The present invention will be described in detail with reference to specific examples.
Example 1
1. Rhodopseudomonas palustris culture
The pseudomonas liquid culture medium formula and the preparation method thereof are as follows: 16.0g/L of gelatin peptone and 10.0g/L, K of trypsin2SO410.0g/L、MgCl21.4g/L, 0.2g/L cetyltrimethylammonium bromide, final pH 7.1 ± 0.2(25 ℃ c)) (ii) a The preparation method comprises the following steps: adding distilled water or deionized water 1L, adding 10mL glycerol, stirring, heating and boiling to dissolve completely, packaging in triangular flask, and autoclaving at 120 deg.C for 20 min.
Culturing rhodopseudomonas palustris: inoculating Rhodopseudomonas palustris (CGMCC No.1.2180) and culturing at 28-32 deg.C for extended period when viable count is higher than 1.0 × 109Stopping the amplification culture at CFU/mL to obtain the rhodopseudomonas palustris liquid.
2. Preparation of microbial organic fertilizer
Mixing and stirring straw, dried pig manure, urea and brown sugar uniformly according to the weight ratio of 2: 0.2: 0.3, adding a proper amount of rhodopseudomonas palustris liquid, adjusting the amount of the added rhodopseudomonas palustris liquid according to the drying degree of the material, controlling the water content to be 30% (preferably, the rhodopseudomonas palustris liquid is easy to agglomerate but does not produce water when being pinched by hand), and finally adding the rhodopseudomonas palustris liquid with the weight of 15% of the total weight of the material, fully stirring uniformly, bagging or barreling for closed anaerobic fermentation at 37 ℃ for 48 hours, after the fermentation is finished, placing the mixture at room temperature for 3 weeks and then maturing to obtain the microbial organic fertilizer, wherein the total viable count is 1.0-5.0 hundred million/g.
Example 2
1. Rhodopseudomonas palustris culture
The pseudomonas liquid culture medium formula and the preparation method thereof are as follows: 16.0g/L of gelatin peptone and 10.0g/L, K of trypsin2SO410.0g/L、MgCl21.4g/L, cetyltrimethylammonium bromide 0.2g/L, final pH 7.1 ± 0.2(25 ℃); the preparation method comprises the following steps: adding distilled water or deionized water 1L, adding 10mL glycerol, stirring, heating and boiling to dissolve completely, packaging into triangular flask, and autoclaving at 121 deg.C for 15 min.
Culturing rhodopseudomonas palustris: inoculating Rhodopseudomonas palustris (CGMCC No.1.2180) and culturing at 28-32 deg.C for extended period when viable count is higher than 1.0 × 109Stopping the amplification culture at CFU/mL to obtain the rhodopseudomonas palustris liquid.
2. Preparation of microbial organic fertilizer
Mixing and uniformly stirring straw, dried cow dung, urea and brown sugar according to the weight ratio of 2.5: 0.3: 0.2, adding a proper amount of rhodopseudomonas palustris liquid, adjusting the amount of the added rhodopseudomonas palustris liquid according to the drying degree of the material, controlling the water content to be 25% (preferably, the rhodopseudomonas palustris liquid is easy to agglomerate but does not produce water) and the weight of the finally added rhodopseudomonas palustris liquid is 5% of the total weight of the material, fully and uniformly stirring, bagging or barreling for closed anaerobic fermentation at 35 ℃ for 56h, after the fermentation is finished, placing the mixture at room temperature for 4 weeks and then maturing to obtain the microbial organic fertilizer, wherein the total viable count is 1.0-5.0 hundred million/g.
Example 3
1. Rhodopseudomonas palustris culture
The pseudomonas liquid culture medium formula and the preparation method thereof are as follows: 16.0g/L of gelatin peptone and 10.0g/L, K of trypsin2SO410.0g/L、MgCl21.4g/L, cetyltrimethylammonium bromide 0.2g/L, final pH 7.1 ± 0.2(25 ℃); the preparation method comprises the following steps: adding distilled water or deionized water 1L, adding 10mL glycerol, stirring, heating and boiling to dissolve completely, packaging in triangular flask, and autoclaving at 122 deg.C for 15 min.
Culturing rhodopseudomonas palustris: inoculating Rhodopseudomonas palustris (CGMCC No.1.2180) and culturing at 28-32 deg.C for extended period when viable count is higher than 1.0 × 109Stopping the amplification culture at CFU/mL to obtain the rhodopseudomonas palustris liquid.
2. Preparation of microbial organic fertilizer
Mixing and stirring straw, dried pig manure, dried cow manure, urea and brown sugar uniformly according to the weight ratio of 2.5: 1: 0.1: 0.5, adding a proper amount of rhodopseudomonas palustris liquid, adjusting the amount of the added rhodopseudomonas palustris liquid according to the drying degree of the material, controlling the water content to be 25% (preferably, the rhodopseudomonas palustris liquid is easy to agglomerate but does not produce water) and the weight of the finally added rhodopseudomonas palustris liquid is 20% of the total weight of the material, fully stirring uniformly, bagging or barreling for closed anaerobic fermentation at 38 ℃ for 36h, standing at room temperature for 2 weeks after the fermentation is finished, and preparing the microbial organic fertilizer, wherein the total viable count is 1.0-3.0 hundred million/g.
Example 4
The microbial organic fertilizer in the embodiments 1, 2 and 3 is used for pepper cultivation, and the specific using method and pepper cultivation method of the microbial organic fertilizer are as follows:
(1) application: selecting a Zanthoxylum bungeanum planting field which is in a karst landform and has been planted with Zanthoxylum bungeanum for 10 years, and mixing a microbial organic fertilizer with soil in a hole application mode in a weight ratio of 5-15% in the first month before the Zanthoxylum bungeanum planting, wherein the weight of the soil in each hole is 0.6-1.5 kg.
(2) Cultivation: and (3) cultivating the peppers according to a conventional mode in the pepper cultivation period, wherein each pepper is cultivated in the central position of the microbial organic fertilizer hole.
(3) Applying a bacterial solution: after the seedlings come out of the soil, the swamp rhodopseudomonas liquid diluted by 100 times is applied to the roots for 1 time.
Comparative example 1
In the pepper planting land of example 4, a refined chicken manure organic fertilizer (hebeiyuan sea biotechnology limited) is selected, the nutrient content of the fertilizer is far beyond the national NY525-2012 standard, the organic matter content is more than or equal to 50%, the nitrogen phosphorus potassium content is more than or equal to 7%, and the composite strain content is 0.4 hundred million/g. The pepper is cultivated according to the conventional method by using the organic fertilizer and the pepper cultivation method in the embodiment 4.
The effect of pepper cultivation in example 4 and comparative example 1 is shown in table 1:
TABLE 1
Figure BDA0002924895950000061
Figure BDA0002924895950000071
As can be seen from the table 1, when the microbial organic fertilizer of the embodiments 1-3 is applied, the emergence rate of the pepper is 86% at most, which is increased by 19% compared with the application of the chicken manure organic fertilizer; meanwhile, the average seedling height is increased by 2.46cm, and the survival rate is improved by 12.6%. Compared with the common farmyard organic fertilizer, the microbial organic fertilizer disclosed by the invention can effectively improve the emergence rate, seedling height and survival rate of pepper cultivation by relieving the continuous cropping obstacle of pepper.
In conclusion, the microbial organic fertilizer can effectively degrade toxic substances secreted into soil by the allelopathy of the pepper, particularly the pepper in karst landform areas, so that the continuous cropping obstacle of the pepper planting land is relieved, the microbial organic fertilizer is suitable for continuous cropping planting of the pepper, and rich nutrients can be provided for the growth of the pepper, and the effect of fertilizing the land is achieved. The microbial organic fertilizer is applied to pepper cultivation, so that the ecological environment of karst landform areas can be recovered by using the characteristics of the karst landform without influencing the economic benefit of people. Certainly, the microbial organic fertilizer can also solve the problem of continuous cropping obstacles of other crops, such as hot peppers, melons, bitter gourds, tomatoes, eggplants and the like.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (10)

1. A microbial organic fertilizer for relieving continuous cropping obstacles of crops is characterized by comprising: the strain is rhodopseudomonas palustris, and the preservation number of the rhodopseudomonas palustris is CGMCC No. 1.2180.
2. The microbial organic fertilizer according to claim 1, which is characterized in that: the culture substrate comprises straws and animal manure, wherein the animal manure is at least one of pig manure and cow manure;
and/or in the microbial organic fertilizer, the rhodopseudomonas palustris is rhodopseudomonas palustris liquid, and the weight of the rhodopseudomonas palustris liquid accounts for 5-20% of the weight of the microbial organic fertilizer.
3. The microbial organic fertilizer according to claim 2, characterized in that: the straws are at least one selected from wheat straws, corn stalks and rice straws, and the pig manure and the cattle manure are dried pig manure and cattle manure;
and/or the using amount of the straws and the animal wastes in the microbial organic fertilizer is equal.
4. The microbial organic fertilizer according to claim 1, which is characterized in that: the microbial organic fertilizer also comprises urea and brown sugar, wherein the weight ratio of the culture medium to the urea to the brown sugar in the microbial organic fertilizer is 4-5: 0.1-0.3: 0.2-05.
5. The preparation method of the microbial organic fertilizer for alleviating the continuous cropping obstacles of the crops as claimed in any one of claims 1 to 4, which is characterized by comprising the following steps: mixing the raw materials according to a weight ratio, uniformly stirring, adding the rhodopseudomonas palustris liquid, fully stirring uniformly, carrying out closed anaerobic fermentation on the materials, and after the fermentation is finished, placing the materials at room temperature for 2-4 weeks and then cooking to obtain the microbial organic fertilizer.
6. The method of claim 5, wherein: adjusting the amount of the rhodopseudomonas palustris liquid according to the dryness of the material, and controlling the water content of the fully and uniformly stirred material to be 25-30%;
and/or the closed anaerobic fermentation conditions are as follows: 35-40 ℃ and 36-56 h;
and/or the total viable count of the microbial organic fertilizer is controlled to be 1.0-5.0 hundred million/g;
and/or the culture method of the rhodopseudomonas palustris liquid comprises the following steps: inoculating Rhodopseudomonas palustris in liquid culture medium of Pseudomonas palustris, and performing amplification culture when viable count is equal to or higher than 1.0 × 109Stopping the amplification culture when the concentration is CFU/mL, and obtaining the rhodopseudomonas palustris liquid.
7. The method of claim 6, wherein: the temperature of the rhodopseudomonas palustris during enlarged culture is 28-32 ℃;
and/or the pseudomonas liquid culture medium formula and the preparation method thereof are as follows: gelatin peptone 16.0g/L, pancreatic eggWhite 10.0g/L, K2SO4 10.0g/L、MgCl21.4g/L and 0.2g/L of hexadecyl trimethylamine bromide, wherein the pH value is 7.1 +/-0.2 at normal temperature, and the preparation method comprises the following steps: taking gelatin peptone, trypsin and K2SO4、MgCl2Adding 1L of distilled water or deionized water into the hexadecyl trimethylamine bromide, adding 10mL of glycerol, stirring, heating and boiling until the mixture is completely dissolved, and carrying out autoclaving at the temperature of 120-.
8. Use of a microbial organic fertilizer according to any one of claims 1-4 and/or prepared by a method according to any one of claims 5-7 for alleviating the sedentary disorders of crops.
9. Use according to claim 8, characterized in that: the crops are selected from at least one of pepper, hot pepper, melon, balsam pear, tomato and eggplant;
and/or the planting land of the crops is karst landform;
and/or the planting land of the crops is the land of Guiyang and/or unitary Yang;
and/or the application method of the microbial organic fertilizer comprises the following steps:
(1) application: in the previous month of crop planting, mixing the microbial organic fertilizer with soil in a hole application mode;
(2) cultivation: in the crop planting period, crops are cultivated in a conventional mode, and each crop is planted in the central position of the microbial organic fertilizer hole application;
(3) applying a bacterial solution: after the crop seedlings come out of the soil, the diluted rhodopseudomonas palustris liquid is applied to the roots for 1 time.
10. Use according to claim 9, characterized in that: the weight ratio of the microbial organic fertilizer to the soil is 5-15: 100, and the weight of the soil in each hole is 0.6-1.5 kg;
and/or diluting the rhodopseudomonas palustris liquid by water by 80-120 times.
CN202110133029.7A 2021-01-29 2021-01-29 Microbial organic fertilizer for relieving crop continuous cropping obstacle and preparation and application thereof Pending CN112759489A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101892157A (en) * 2010-08-18 2010-11-24 孙廷震 Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof
CN104788244A (en) * 2015-05-04 2015-07-22 冉洪崎 Organic fertilizer and preparation method thereof
CN107541477A (en) * 2017-09-13 2018-01-05 河北然生物科技有限公司 A kind of method using streptococcus acidi lactici fermented solution culture photosynthetic bacteria
CN108569921A (en) * 2017-03-07 2018-09-25 庆阳敦博科技发展有限公司 A kind of Chinese prickly ash long-acting special biological organic fertilizer and preparation method thereof
CN108893418A (en) * 2018-05-10 2018-11-27 湖南省植物保护研究所 A kind of Rhodopseudomonas palustris LY-6 microbial inoculum and preparation and its application to the prevention and treatment of tomato southern blight
CN109315117A (en) * 2018-10-23 2019-02-12 贵州师范大学 One kind spreading fertilizer over the fields method for Karst region bloompepper forest organic fertilizer

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101892157A (en) * 2010-08-18 2010-11-24 孙廷震 Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof
CN104788244A (en) * 2015-05-04 2015-07-22 冉洪崎 Organic fertilizer and preparation method thereof
CN108569921A (en) * 2017-03-07 2018-09-25 庆阳敦博科技发展有限公司 A kind of Chinese prickly ash long-acting special biological organic fertilizer and preparation method thereof
CN107541477A (en) * 2017-09-13 2018-01-05 河北然生物科技有限公司 A kind of method using streptococcus acidi lactici fermented solution culture photosynthetic bacteria
CN108893418A (en) * 2018-05-10 2018-11-27 湖南省植物保护研究所 A kind of Rhodopseudomonas palustris LY-6 microbial inoculum and preparation and its application to the prevention and treatment of tomato southern blight
CN109315117A (en) * 2018-10-23 2019-02-12 贵州师范大学 One kind spreading fertilizer over the fields method for Karst region bloompepper forest organic fertilizer

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