CN112755110A - Preparation method for improving content of saponins in Qizhen capsule - Google Patents

Preparation method for improving content of saponins in Qizhen capsule Download PDF

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Publication number
CN112755110A
CN112755110A CN202011340029.6A CN202011340029A CN112755110A CN 112755110 A CN112755110 A CN 112755110A CN 202011340029 A CN202011340029 A CN 202011340029A CN 112755110 A CN112755110 A CN 112755110A
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China
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traditional chinese
chinese medicine
extract
ethanol
qizhen
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CN202011340029.6A
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Chinese (zh)
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王强
危凤
阮晓
杨丽
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Ningbo University of Technology
Zhejiang University of Science and Technology ZUST
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Zhejiang University of Science and Technology ZUST
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
    • A61K36/195—Strobilanthes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/85—Verbenaceae (Verbena family)
    • A61K36/855—Clerodendrum, e.g. glorybower
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
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  • Alternative & Traditional Medicine (AREA)
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  • Marine Sciences & Fisheries (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)
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Abstract

The invention discloses a method for improving the content of saponin substances in the preparation of Qizhen capsules, wherein traditional Chinese medicine powder is arranged in a supercritical extraction kettle, under the conditions of optimized supercritical fluid pressure, temperature, entrainer flow rate and extraction time parameters, the composition of the entrainer is designed according to the physicochemical properties of a target extract, and the plant saponin substances in the extract are selectively improved.

Description

Preparation method for improving content of saponins in Qizhen capsule
Technical Field
The invention relates to preparation of Qizhen capsules, in particular to a preparation method for improving the content of saponin substances in Qizhen capsules.
Background
The Qizhen capsule is prepared from five traditional Chinese medicines including astragalus root, pearl, notoginseng, dyers woad leaf and paris polyphylla, has the effects of tonifying qi, removing blood stasis, clearing heat and detoxicating, and achieves the aim of adjuvant therapy of malignant tumors by strengthening body resistance, eliminating evil and improving the life quality of tumor patients. The patent application with publication number CN1376497 discloses a astragalus membranaceus capsule and a preparation method thereof, wherein the preparation method of the astragalus membranaceus capsule comprises the steps of firstly decocting astragalus membranaceus, concentrating decoction, soaking in ethanol, drying extract, crushing, and sieving to obtain fine powder of the astragalus membranaceus; soaking Margarita in ethanol, and pulverizing into fine powder; pulverizing Notoginseng radix, folium Isatidis and rhizoma paridis together, soaking in ethanol, concentrating the filtrate, adding Margarita fine powder, mixing, drying, pulverizing into fine powder, adding radix astragali fine powder, mixing, and encapsulating to obtain QIZHEN Capsule.
Disclosure of Invention
The invention aims to solve the technical problem of providing a preparation method for improving the content of saponin substances in Qizhen capsules.
The technical scheme adopted by the invention for solving the technical problems is as follows: the technical scheme adopted by the invention for solving the technical problems is as follows: the method for improving the content of saponin substances in the preparation of the Qizhen capsule comprises the following steps:
a. mixing radix astragali, pseudo-ginseng, folium isatidis and rhizoma paridis according to a mass ratio of 10-12: 1-3: 3-5: 2-4, crushing the prepared traditional Chinese medicine raw materials, and sieving the crushed traditional Chinese medicine raw materials with a 45-60-mesh sieve to obtain mixed traditional Chinese medicine powder;
b. the Chinese herbal medicine powder is filled into a supercritical extraction kettle for extraction, the extraction temperature is 35-55 ℃, and CO is used for extraction2The fluid pressure is 30-60 MPa, the flow rate of the entrainer is 10-50 ml/min, the extraction time is 2-4 h, the extract is absorbed by ethanol to form ethanol absorption liquid, and the volume mass ratio of the ethanol to the traditional Chinese medicine raw materials is 1 ml: 2g, the entrainer is prepared from cyclohexane, ethanol and water according to a mass ratio of 3-5: 2-4: 2-4; the entrainer can extract saponins from medicinal materials completely, and has no influence on other substances;
c. And (3) concentrating the ethanol absorption liquid under the pressure of 450-500 Pa to dryness to obtain an extract, and preparing the extract and pearl powder with equal mass into a traditional Chinese medicine preparation Qizhen capsule. Measuring total saponin content with panaxadiol as reference by ultraviolet spectrophotometry.
Compared with the prior art, the method has the advantages that the content of saponin substances in the preparation of the stilbene-pearl capsule is improved, the traditional Chinese medicine powder is arranged in a supercritical extraction kettle, the entrainer composition is designed according to the physicochemical properties of a target extract under the conditions of optimized supercritical fluid pressure, temperature, entrainer flow rate and extraction time parameters, the plant saponin substances in the extract are selectively improved, the ultrasonic extraction operation is simple, the plant saponin substances are fully extracted, the plant saponins in the obtained extract are mainly ginsenoside, notoginsenoside and paris polyphylla saponin, the extract is mixed with equal amount of pearl powder to prepare the stilbene-pearl capsule, and the stilbene-pearl capsule has a therapeutic effect on W256 carcinosarcoma of rats.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
1000g of traditional Chinese medicine raw materials prepared from astragalus, pseudo-ginseng, folium isatidis and rhizoma paridis according to the mass ratio of 11: 2: 4: 3 are crushed, sieved by a sieve of 45-60 meshes to obtain traditional Chinese medicine powder, and then the traditional Chinese medicine powder is put into a supercritical extraction kettle, the extraction temperature is 40 ℃, and CO is added 2The fluid pressure is 40MPa, the flow rate of entrainer is 35ml/min, the extraction time is 3h, the extract is absorbed by 500ml ethanol, the entrainer comprises cyclohexane, ethanol and water with the mass ratio of 4: 3, the extract is obtained by concentrating under reduced pressure of 500Pa until the absorption liquid is nearly dry, 98g of extract is obtained, the content of saponins in the extract is 35%, and the extract and pearl powder with equal mass are prepared into the traditional Chinese medicine preparation Qizhen capsule.
Example 2
The method is basically the same as the example 1, except that 1000g of traditional Chinese medicine raw materials prepared from astragalus, pseudo-ginseng, dyers woad leaf and paris polyphylla according to the mass ratio of 12: 3: 5: 4 are adopted, the entrainer comprises cyclohexane, ethanol and water according to the mass ratio of 5: 2, 500Pa is decompressed and concentrated to be dry in absorption liquid, 90g of extract is obtained, the content of saponin substances of the extract is 32%, and the extract and pearl powder with equal mass are prepared into the traditional Chinese medicine preparation Qizhen capsule.
Example 3
The method is basically the same as the example 1, except that 1000g of traditional Chinese medicine raw materials prepared from astragalus, pseudo-ginseng, dyers woad leaf and paris polyphylla according to the mass ratio of 10: 1: 3: 2 are adopted, the entrainer comprises cyclohexane, ethanol and water according to the mass ratio of 3: 4, 500Pa is decompressed and concentrated to be dry in absorption liquid, 102g of extract is obtained, the content of saponin substances of the extract is 22%, and the extract and pearl powder with equal mass are prepared into the traditional Chinese medicine preparation Qizhen capsule.
Test examples
For rat carcinosarcoma W256 tumor inhibition rate experiment, take C57150 BL/6 rats, randomly divided into 15 groups, 3 groups of 4.5 and 1.5g/kg po × 10pd and 1.5 and 0.75g/kg ip × 10pd were administered first for 3 days, on day 4 all animals were inoculated with a vigorous source of W256 tumor while administration was continued, and ascites fluid was withdrawn under sterile conditions and diluted to 2 × 10 with physiological saline7The tumor inhibition rate was calculated from the following formula by dissecting out the tumor and weighing it after ten days. The tumor inhibition rate was (1-mean tumor weight of administration group/mean tumor weight of control group) × 100%, and the tumor inhibition rates of the oral administration high dose groups were 41.01%, 39.11%, 40.01%; the tumor inhibition rate of the low-dose group is 34.04%, 31.98% and 30.00%. The tumor inhibition rates of the intraperitoneal administration high-dose groups are 42.06, 41.04 and 43.92; the tumor inhibition rate of the low-dose group is 31.04%, 33.00% and 34.19%.

Claims (1)

1. The method for improving the content of saponin substances in the preparation of the Qizhen capsule is characterized by comprising the following steps:
a. mixing radix astragali, pseudo-ginseng, folium isatidis and rhizoma paridis according to a mass ratio of 10-12: 1-3: 3-5: 2-4, crushing the prepared traditional Chinese medicine raw materials, and sieving the crushed traditional Chinese medicine raw materials with a 45-60-mesh sieve to obtain mixed traditional Chinese medicine powder;
b. The Chinese herbal medicine powder is filled into a supercritical extraction kettle for extraction, the extraction temperature is 35-55 ℃, and CO is used for extraction2The fluid pressure is 30-60 MPa, the flow rate of the entrainer is 10-50 ml/min, the extraction time is 2-4 h, the extract is absorbed by ethanol to form ethanol absorption liquid, and the volume mass ratio of the ethanol to the traditional Chinese medicine raw materials is 1 ml: 2 g, the entrainer is prepared from cyclohexane, ethanol and water according to a mass ratio of 3-5: 2-4: 2-4;
c. and (3) concentrating the ethanol absorption liquid under the pressure of 450-500 Pa to dryness to obtain an extract, and preparing the extract and pearl powder with equal mass into a traditional Chinese medicine preparation Qizhen capsule.
CN202011340029.6A 2020-11-25 2020-11-25 Preparation method for improving content of saponins in Qizhen capsule Pending CN112755110A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1376497A (en) * 2002-03-04 2002-10-30 汪贵昌 Qizhen capsule and its preparing process
CN109512879A (en) * 2018-12-20 2019-03-26 宁波泰康红豆杉生物工程有限公司 The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation
CN109602803A (en) * 2018-12-20 2019-04-12 浙江大学宁波理工学院 The method that limonin content improves in loose Pueraria lobota anti-trioxypurine particle preparation
CN109602802A (en) * 2018-12-20 2019-04-12 浙江大学宁波理工学院 The method that flavones content improves in loose Pueraria lobota anti-trioxypurine particle preparation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1376497A (en) * 2002-03-04 2002-10-30 汪贵昌 Qizhen capsule and its preparing process
CN109512879A (en) * 2018-12-20 2019-03-26 宁波泰康红豆杉生物工程有限公司 The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation
CN109602803A (en) * 2018-12-20 2019-04-12 浙江大学宁波理工学院 The method that limonin content improves in loose Pueraria lobota anti-trioxypurine particle preparation
CN109602802A (en) * 2018-12-20 2019-04-12 浙江大学宁波理工学院 The method that flavones content improves in loose Pueraria lobota anti-trioxypurine particle preparation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
叶勇: "《制药工艺学》", 28 February 2014, 华南理工大学出版社 *

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