CN109512879A - The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation - Google Patents

The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation Download PDF

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CN109512879A
CN109512879A CN201811562975.8A CN201811562975A CN109512879A CN 109512879 A CN109512879 A CN 109512879A CN 201811562975 A CN201811562975 A CN 201811562975A CN 109512879 A CN109512879 A CN 109512879A
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trioxypurine
extract
loose
pueraria lobota
isoflavone content
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王强
戚军
阮晓
郑波
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TAIKANG TAXUS CHINENSIS BIOLOGICAL ENGINEERING Co Ltd NINGBO CITY
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TAIKANG TAXUS CHINENSIS BIOLOGICAL ENGINEERING Co Ltd NINGBO CITY
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses the methods that isoflavone content in loose Pueraria lobota anti-trioxypurine particle preparation improves, pass through supercritical extract, wherein entrainer is formulated according to quality than range 5:1:4 by ethyl alcohol, petroleum ether, water, extract isoflavones abundant, methanol absorption, absorbing liquid chromatographs crude separation with macroreticular resin, obtains faint yellow loose Pueraria lobota anti-trioxypurine extract, then use Al3+The analysis of rutin binary complex fluorimetry, isoflavone content is 170~200mg/g in loose Pueraria lobota anti-trioxypurine extract, isoflavones monomer component are as follows: Puerarin, Puerarin -7- xyloside, daidzein, daidzin, 4 ', 6 "-diacetyl Puerarin; extracting method of the invention is simple; isoflavones extracts sufficiently, and extract main ingredient also has stronger anti-trioxypurine activity.

Description

The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation
Technical field
The present invention relates to the improvement methods of active constituent content in Chinese herbal granules preparation, and in particular to loose Pueraria lobota anti-trioxypurine The method that isoflavone content improves in grain preparation.
Background technique
Hyperuricemia (Hyperuricemia, HUA) is one group disorderly with heredity and (or) acquired internal purine metabolism Unrest and (or) uric acid (UA) excretion reduce caused disease.When biosynthesis of purine excessive, internal rich in purine food intake When obstacle and renal tubule reduce the clearance rate of lithate, it may occur in which that circulation Uric Acid Content increases, lead to antihyperuricemic The morbidity of disease.For loose Pueraria lobota anti-trioxypurine particle by pine needle, pueraria lobata, three taste Chinese medicinal composition of dried orange peel, pine needle is Pinaceae pine genus plant masson pinePinus massoniana Lamb. dried leaf, whole year can adopt, and remove impurity, and sun drying or low temperature drying is used, it is cut into about 4~ The section of 10mm.Pueraria lobata source are as follows: legume pueraria lobataPueraria lobata (Willd.) dry root of Ohwi is practised and claims open country Pueraria lobota, autumn, the excavation of two season of winter take advantage of fresh-cut into the fritter of side length about 5~12mm, drying.Dried orange peel source are as follows: rutaceae orangeCitrus reticulate Blanco and its dry mature skin of variety, medicinal material are divided into " dried orange peel " and " citrus chachiensis hortorum ", Harvesting ripe fruit strips pericarp, chopping, sun drying or low temperature drying.Coastal Area in Zhejiang Province especially Ningbo area it is civil with Tea bag form is taken, dispel pathogenic wind and remove dampness, be clearing heat and detoxicating, strengthening the spleen and reducing phlegm the effect of, cure mainly phlegm stagnation (wet turbid), retention of damp-heat in the interior Type hyperuricemia.Loose Pueraria lobota anti-trioxypurine particle is exactly to be crushed with pine needle, pueraria lobata, dried orange peel, extract effective ingredient, along with medicinal Auxiliary material is prepared.
Summary of the invention
Technical problem to be solved by the invention is to provide the sides that isoflavone content in loose Pueraria lobota anti-trioxypurine particle preparation improves Method, this method are extracted simply, and isoflavones extracts sufficiently, and main ingredient also has stronger anti-trioxypurine activity in extract.
The technical scheme of the invention to solve the technical problem is: isoflavones contains in loose Pueraria lobota anti-trioxypurine particle preparation The method improved is measured, its step are as follows:
A, the traditional Chinese medicinal material raw materials for preparing pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 crush Afterwards, 45~60 meshes are crossed, mixed traditional Chinese medicinal material raw materials powder is fitted into supercritical extraction reactor；
B, it is extracted in supercritical extraction reactor, extraction temperature is 35~55 DEG C, CO2Fluid pressure is 30~60MPa, entrainer stream 0.1~0.6ml/min of speed, 2~4h of extraction time, extract is absorbing liquid, methanol and traditional Chinese medicinal material raw materials powder with methanol absorption Volume mass ratio be 1ml:2g, the entrainer is formulated according to quality than range 5:1:4 by ethyl alcohol, petroleum ether, water；It should Entrainer can extract isoflavone like substance in medicinal material sufficiently, and other materials decrease instead；
C, absorbing liquid with D101 type macroreticular resin chromatograph, per hour the distilled water of two bed volume flow velocitys be eluted to efflux without Color, then pillar is washed with the ethyl alcohol of the mass percentage concentration 45% of 10 bed volumes, ethanol eluate is under the conditions of 450~500Pa It is concentrated under reduced pressure into and closely does, 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract.Weighing, then loose Pueraria lobota anti-trioxypurine mentions Take object using Al3+The analysis of rutin binary complex fluorimetry, excitation spectrum bandwidth 3nm, excitation wavelength lambdaex436nm, hair Penetrate spectral bandwidth 5nm, λem483nm, rutin standard solution fluorescence intensity map quantitatively to respective concentration, obtain isoflavone content The loose Pueraria lobota anti-trioxypurine extract of raising, then Chinese materia medica preparation pine Pueraria lobota anti-trioxypurine particle is configured to pharmaceutic adjuvant.
Compared with the prior art, the advantages of the present invention are as follows the sides that isoflavone content in loose Pueraria lobota anti-trioxypurine particle preparation improves Method, by supercritical extract, wherein entrainer is formulated according to quality than range 5:1:4 by ethyl alcohol, petroleum ether, water, is made different Extracting flavonoids are abundant, methanol absorption, and absorbing liquid chromatographs crude separation with macroreticular resin, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract, Al is used again3+The analysis of rutin binary complex fluorimetry, in loose Pueraria lobota anti-trioxypurine extract isoflavone content be 170~ 200mg/g, isoflavones monomer component are as follows: Puerarin, Puerarin -7- xyloside, daidzein, daidzin, 4 ', 6 "-diacetyl Pueraria lobotas Root element, extracting method of the invention is simple, and isoflavones extracts sufficiently, and extract main ingredient also has stronger anti-trioxypurine living Property.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
Embodiment 1
The traditional Chinese medicinal material raw materials 100g that pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 are prepared, powder After broken, 45~60 meshes are crossed, Chinese drugs powder is obtained, is fitted into supercritical extraction reactor and extracts, extraction temperature is 48 DEG C, CO2Stream Body pressure is 45MPa, and entrainer flow velocity 0.48ml/min, entrainer is by ethyl alcohol, petroleum ether, water according to quality than range 5:1:4 It is formulated, which can extract isoflavone like substance in medicinal material sufficiently, and other materials decrease instead, extraction time 2.8h, extract are absorbing liquid with 50ml methanol absorption, and absorbing liquid is chromatographed with D101 type macroreticular resin, per hour two column bed bodies The distilled water of product flow velocity is eluted to that efflux is colourless, then washes pillar, second with 45% ethyl alcohol of mass percentage concentration of 10 bed volumes Alcohol eluen is concentrated under reduced pressure under the conditions of 450~500Pa closely to be done, and 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine and mention Object 2.57g is taken, loose Pueraria lobota anti-trioxypurine extract uses Al3+The analysis of rutin binary complex fluorimetry, excitation spectrum bandwidth 3nm, excitation wavelength lambdaex436nm, emission spectrum bandwidth 5nm, λem483nm, rutin standard solution fluorescence intensity is to respective concentration Mapping is quantitative, and isoflavones components content is 184 mg/g in extract, then is configured to Chinese materia medica preparation pine Pueraria lobota drop with pharmaceutic adjuvant and urinates Granulates.
Embodiment 2
Substantially the same manner as Example 1, different only extraction temperatures are 35 DEG C, CO2Fluid pressure is 30MPa, entrainer stream Fast 0.1ml/min, extraction time 4h obtain faint yellow loose Pueraria lobota anti-trioxypurine extract 2.51g, isoflavones components content in extract For 196 mg/g.
Embodiment 3
Substantially the same manner as Example 1, different only extraction temperatures are 55 DEG C, CO2Fluid pressure is 60MPa, entrainer stream Fast 0.6ml/min, extraction time 2h obtain faint yellow loose Pueraria lobota anti-trioxypurine extract 2.53g, isoflavones components content in extract For 175 mg/g.
Test example 1
The influence that hyperuricemia model rat is caused to adenine+ethambutol, gives adenine and ethamine using continuous gavage Rat hyperuricemia model is established within butanol 21 days, loose Pueraria lobota anti-trioxypurine extract (5.25 g extracts/kg, 3.50 is given in stomach-filling G extract/kg, 1.75 g extracts/kg), successive administration 21 days, loose three dosage of Pueraria lobota anti-trioxypurine extract (5.25,3.50, 1.75 g/kg) rat model serum UA, Cr, BUN, TC, TG content can be reduced, serum and liver XOD, ADA activity are reduced, The whole blood viscosity under high shear rate is reduced, serum NO levels are increased；Loose Pueraria lobota anti-trioxypurine extract (5.25,3.50 g/kg) can be dropped Low plasma viscosity, loose Pueraria lobota anti-trioxypurine extract (3.50 g/kg) can reduce AI.
Test example 2
The influence that antihyperuricemic disease mouse model is caused to xanthine+ethambutol, gives xanthine and ethamine using continuous gavage Studies on Animal Models of Hyperuricemic Mice is established within butanol 16 days, loose Pueraria lobota anti-trioxypurine extract (7.5 g extracts/kg, 5.0 g is given in stomach-filling Extract/kg, 2.5 g extracts/kg), successive administration 16 days, loose three dosage of Pueraria lobota anti-trioxypurine extract (7.5,5.0,2.5g/ Kg model mice serum UA, Cr, TG content and AI) can be reduced, serum hdl-c content is increased.

Claims (2)

1. the method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation, it is characterised in that steps are as follows:
A, the traditional Chinese medicinal material raw materials for preparing pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 crush Afterwards, 45~60 meshes are crossed, mixed traditional Chinese medicinal material raw materials powder is fitted into supercritical extraction reactor；
B, it is extracted in supercritical extraction reactor, extraction temperature is 35~55 DEG C, CO2Fluid pressure is 30~60MPa, entrainer stream 0.1~0.6ml/min of speed, 2~4h of extraction time, extract is absorbing liquid, methanol and traditional Chinese medicinal material raw materials powder with methanol absorption Volume mass ratio be 1ml:2g, the entrainer is formulated according to quality than range 5:1:4 by ethyl alcohol, petroleum ether, water；
C, absorbing liquid with D101 type macroreticular resin chromatograph, per hour the distilled water of two bed volume flow velocitys be eluted to efflux without Color, then pillar is washed with the ethyl alcohol of the mass percentage concentration 45% of 10 bed volumes, ethanol eluate is under the conditions of 450~500Pa It is concentrated under reduced pressure into and closely does, 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract.
2. the method that isoflavone content improves in pine Pueraria lobota anti-trioxypurine particle preparation as described in claim 1, it is characterised in that step Extraction temperature is 48 DEG C in rapid b, CO2Fluid pressure is 45MPa, entrainer ethyl alcohol flow velocity 0.48ml/min, extraction time 2.8h.
CN201811562975.8A 2018-12-20 2018-12-20 The method that isoflavone content improves in loose Pueraria lobota anti-trioxypurine particle preparation Pending CN109512879A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112755110A (en) * 2020-11-25 2021-05-07 浙大宁波理工学院 Preparation method for improving content of saponins in Qizhen capsule

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103191235A (en) * 2013-03-29 2013-07-10 宁波泰康红豆杉生物工程有限公司 Traditional Chinese medicine compound extract for preventing and treating hyperuricemia and application

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103191235A (en) * 2013-03-29 2013-07-10 宁波泰康红豆杉生物工程有限公司 Traditional Chinese medicine compound extract for preventing and treating hyperuricemia and application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
唐德智: "黄酮类化合物的提取、分离、纯化研究进展", 《中药与天然药物》 *
宋航: "《制药分离工程》", 31 August 2011, 华东理工大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112755110A (en) * 2020-11-25 2021-05-07 浙大宁波理工学院 Preparation method for improving content of saponins in Qizhen capsule

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Application publication date: 20190326