CN109512878A - The method that thick grass acid content improves in loose Pueraria lobota anti-trioxypurine particle preparation - Google Patents
The method that thick grass acid content improves in loose Pueraria lobota anti-trioxypurine particle preparation Download PDFInfo
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- CN109512878A CN109512878A CN201811562974.3A CN201811562974A CN109512878A CN 109512878 A CN109512878 A CN 109512878A CN 201811562974 A CN201811562974 A CN 201811562974A CN 109512878 A CN109512878 A CN 109512878A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
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- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses the methods that thick grass acid content in loose Pueraria lobota anti-trioxypurine particle preparation improves, pass through supercritical extract, wherein entrainer is by by ethyl alcohol, hexamethylene, petroleum ether, water is formulated according to mass ratio 5:1:1:3, shikimic acid extracts abundant, the absorbing liquid of methanol absorption chromatographs crude separation with macroreticular resin, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract, with UPLC- diode array detector standard substance quantified by external standard method, thick grass acid content is 17.0~18.3mg/g in loose Pueraria lobota anti-trioxypurine extract, extracting method of the invention is simple, loose Pueraria lobota anti-trioxypurine extract thick grass acid content improves, extract main ingredient also has stronger anti-trioxypurine activity.
Description
Technical field
The present invention relates to the improvement methods of active constituent content in Chinese herbal granules preparation, and in particular to loose Pueraria lobota anti-trioxypurine
The method that thick grass acid content improves in grain preparation.
Background technique
Hyperuricemia (Hyperuricemia, HUA) is one group disorderly with heredity and (or) acquired internal purine metabolism
Unrest and (or) uric acid (UA) excretion reduce caused disease.When biosynthesis of purine excessive, internal rich in purine food intake
When obstacle and renal tubule reduce the clearance rate of lithate, it may occur in which that circulation Uric Acid Content increases, lead to antihyperuricemic
The morbidity of disease.For loose Pueraria lobota anti-trioxypurine particle by pine needle, pueraria lobata, three taste Chinese medicinal composition of dried orange peel, pine needle is Pinaceae pine genus plant masson pinePinus massoniana Lamb. dried leaf, whole year can adopt, and remove impurity, and sun drying or low temperature drying is used, it is cut into about 4~
The section of 10mm.Pueraria lobata source are as follows: legume pueraria lobataPueraria lobata (Willd.) dry root of Ohwi is practised and claims open country
Pueraria lobota, autumn, the excavation of two season of winter take advantage of fresh-cut into the fritter of side length about 5~12mm, drying.Dried orange peel source are as follows: rutaceae orangeCitrus reticulate Blanco and its dry mature skin of variety, medicinal material are divided into " dried orange peel " and " citrus chachiensis hortorum ",
Harvesting ripe fruit strips pericarp, chopping, sun drying or low temperature drying.Coastal Area in Zhejiang Province especially Ningbo area it is civil with
Tea bag form is taken, dispel pathogenic wind and remove dampness, be clearing heat and detoxicating, strengthening the spleen and reducing phlegm the effect of, cure mainly phlegm stagnation (wet turbid), retention of damp-heat in the interior
Type hyperuricemia.Loose Pueraria lobota anti-trioxypurine particle is exactly to be crushed with pine needle, pueraria lobata, dried orange peel, extract effective ingredient, along with medicinal
Auxiliary material is prepared.
Summary of the invention
Technical problem to be solved by the invention is to provide the sides that thick grass acid content in loose Pueraria lobota anti-trioxypurine particle preparation improves
Method, this method are extracted simply, and shikimic acid extracts sufficiently, and main ingredient also has stronger anti-trioxypurine in loose Pueraria lobota anti-trioxypurine extract
Activity.
The technical scheme of the invention to solve the technical problem is: shikimic acid contains in loose Pueraria lobota anti-trioxypurine particle preparation
The method improved is measured, its step are as follows:
A, the traditional Chinese medicinal material raw materials for preparing pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 crush
Afterwards, 45~60 meshes are crossed, traditional Chinese medicinal material raw materials powder is obtained, is fitted into supercritical extraction reactor；
B, it is extracted in supercritical extraction reactor, extraction temperature is 35~55 DEG C, CO2Fluid pressure is 30~60MPa, entrainer stream
0.1~0.6ml/min of speed, 2~4h of extraction time, extract is absorbing liquid, methanol and traditional Chinese medicinal material raw materials powder with methanol absorption
Volume mass ratio be 1ml:2g；The entrainer by ethyl alcohol, hexamethylene, petroleum ether, water according to mass ratio 5:1:1:3 prepare and
At；Shikimic acid in medicinal material can be proposed that sufficiently other compositions decrease instead by the entrainer；
C, absorbing liquid with D101 type macroreticular resin chromatograph, per hour the distilled water of two bed volume flow velocitys be eluted to efflux without
Color, then pillar is washed with the ethyl alcohol of the mass percentage concentration 75% of 10 bed volumes, ethanol eluate is under the conditions of 450~500Pa
It is concentrated under reduced pressure into and closely does, 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract.Weighing, then loose Pueraria lobota anti-trioxypurine mentions
It takes object using UPLC- diode array detector standard substance quantified by external standard method, obtains the higher loose Pueraria lobota drop urine of thick grass acid content
Sour extract, then Chinese materia medica preparation pine Pueraria lobota anti-trioxypurine particle is configured to pharmaceutic adjuvant.
Compared with the prior art, the advantages of the present invention are as follows the sides that thick grass acid content in loose Pueraria lobota anti-trioxypurine particle preparation improves
Method, by supercritical extract, wherein entrainer by being prepared by ethyl alcohol, hexamethylene, petroleum ether, water according to mass ratio 5:1:1:3 and
At shikimic acid extracts sufficiently, and the absorbing liquid of methanol absorption chromatographs crude separation with macroreticular resin, obtains faint yellow loose Pueraria lobota anti-trioxypurine and mentions
Object is taken, with UPLC- diode array detector standard substance quantified by external standard method, thick grass acid content is in loose Pueraria lobota anti-trioxypurine extract
17.0~18.3mg/g, extracting method of the invention is simple, and loose Pueraria lobota anti-trioxypurine extract thick grass acid content improves, and extract is main
Composition also has stronger anti-trioxypurine activity.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
Embodiment 1
The traditional Chinese medicinal material raw materials 100g that pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 are prepared, powder
After broken, 45~60 meshes to be crossed, traditional Chinese medicinal material raw materials powder is obtained, is fitted into supercritical extraction reactor and extracts, extraction temperature is 45 DEG C,
CO2Fluid pressure is 43MPa, and entrainer flow velocity 0.45ml/min, entrainer is by ethyl alcohol, hexamethylene, petroleum ether, water according to quality
It is formulated than 5:1:1:3, which can propose shikimic acid in medicinal material sufficiently, extraction time 2.6h, extract 50ml
Methanol absorption is absorbing liquid, and absorbing liquid is chromatographed with D101 type macroreticular resin, per hour the distillation washing of two bed volume flow velocitys
Take off colourless to efflux, then wash pillar with 75% ethyl alcohol of mass percentage concentration of 10 bed volumes, ethanol eluate 450~
It is concentrated under reduced pressure under the conditions of 500Pa and closely does, 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract 1.94g, loose Pueraria lobota drop
Uric acid extract uses UPLC- diode array detector standard substance quantified by external standard method, shikimic acid component content in extract
For 17.8 mg/g, therefore shikimic acid extracts sufficiently, then is configured to Chinese materia medica preparation pine Pueraria lobota anti-trioxypurine particle with pharmaceutic adjuvant.
Embodiment 2
Substantially the same manner as Example 1, different only extraction temperatures are 35 DEG C, CO2Fluid pressure is 30MPa, entrainer stream
Fast 0.1ml/min, extraction time 4h, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract 1.92g, and extract species thick grass sour component contains
Amount is 17.6 mg/g.
Embodiment 3
Substantially the same manner as Example 1, different only extraction temperatures are 55 DEG C, CO2Fluid pressure is 60MPa, entrainer stream
Fast 0.6ml/min, extraction time 2h, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract 1.91g, and extract species isoflavones components contain
Amount is 17.5 mg/g.
Test example 1
The influence that hyperuricemia model mouse is caused to yeast extract+Oteracil Potassium gives yeast extract 8 days using continuous gavage and end
Secondary intraperitoneal injection Oteracil Potassium establishes Studies on Animal Models of Hyperuricemic Mice, and loose Pueraria lobota anti-trioxypurine extract is given in stomach-filling, and (7.5 g are extracted
Object/kg, 5.0 g extracts/kg, 2.5 g extracts/kg), successive administration 8 days, loose Pueraria lobota anti-trioxypurine extract 7.5g/kg can drop
Low hyperuricemia model mice serum UA, Cr, BUN content reduces serum and liver XOD, liver ADA activity, loose Pueraria lobota anti-trioxypurine
5.0 g/kg of extract can reduce serum UA, Cr, BUN, TC, TG content, reduce serum XOD, serum and liver ADA activity, loose Pueraria lobota
2.5 g/kg of anti-trioxypurine extract can reduce serum UA, BUN, TG content, reduce liver ADA activity.
Test example 2
Influence to high purine feed cause hyperuricemia model rat is fed establishes rat in 27 days using high purine feed is continuously fed
Loose Pueraria lobota anti-trioxypurine extract (5.25 g extracts/kg, 3.50 g extracts/kg, 1.75 are given in hyperuricemia model, stomach-filling
G extract/kg), successive administration 27 days, loose three dosage of Pueraria lobota anti-trioxypurine extract (5.25,3.50,1.75 g/kg) can be dropped
Low rat model serum UA, Cr, BUN, TC, TG content reduces serum and liver XOD, serum ADA activity, reduces basic, normal, high
Whole blood viscosity and plasma viscosity under shear rate increase serum NO levels, loose Pueraria lobota anti-trioxypurine extract (3.50,1.75 g/kg)
Liver ADA activity can be reduced.
Claims (2)
1. the method that thick grass acid content improves in loose Pueraria lobota anti-trioxypurine particle preparation, it is characterised in that steps are as follows:
A, the traditional Chinese medicinal material raw materials for preparing pine needle, pueraria lobata, dried orange peel 4.5~4.75:4.5 in mass ratio~4.75:0.5~1 crush
Afterwards, 45~60 meshes are crossed, traditional Chinese medicinal material raw materials powder is obtained, is fitted into supercritical extraction reactor；
B, it is extracted in supercritical extraction reactor, extraction temperature is 35~55 DEG C, CO2Fluid pressure is 30~60MPa, entrainer stream
0.1~0.6ml/min of speed, 2~4h of extraction time, extract is absorbing liquid, methanol and traditional Chinese medicinal material raw materials powder with methanol absorption
Volume mass ratio be 1ml:2g；The entrainer by ethyl alcohol, hexamethylene, petroleum ether, water according to mass ratio 5:1:1:3 prepare and
At；
C, absorbing liquid with D101 type macroreticular resin chromatograph, per hour the distilled water of two bed volume flow velocitys be eluted to efflux without
Color, then pillar is washed with the ethyl alcohol of the mass percentage concentration 75% of 10 bed volumes, ethanol eluate is under the conditions of 450~500Pa
It is concentrated under reduced pressure into and closely does, 105 DEG C of dryings for 24 hours, obtain faint yellow loose Pueraria lobota anti-trioxypurine extract.
2. the method that thick grass acid content improves in pine Pueraria lobota anti-trioxypurine particle preparation as described in claim 1, it is characterised in that step
In rapid b, extraction temperature is 45 DEG C, CO2Fluid pressure is 43MPa, entrainer ethyl alcohol flow velocity 0.45ml/min, extraction time
2.6h。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112043599A (en) * | 2020-09-02 | 2020-12-08 | 广东微纳生物科技有限公司 | Method for preparing uric acid-reducing powder by nanotechnology and processing equipment |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191235A (en) * | 2013-03-29 | 2013-07-10 | 宁波泰康红豆杉生物工程有限公司 | Traditional Chinese medicine compound extract for preventing and treating hyperuricemia and application |
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- 2018-12-20 CN CN201811562974.3A patent/CN109512878A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191235A (en) * | 2013-03-29 | 2013-07-10 | 宁波泰康红豆杉生物工程有限公司 | Traditional Chinese medicine compound extract for preventing and treating hyperuricemia and application |
Non-Patent Citations (4)
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| JOHANNA SCHEUBA, ET AL.: "fast and green-CO2 based extraction, isolation, and quantification of phenolic styrax constituents", 《PLANTA MED》 * |
| 宋航: "《制药分离工程》", 31 August 2011, 华东理工大学出版社 * |
| 张迎等: "八角莽草酸提取、分离、纯化及其功能性研究进展", 《粮食与油脂》 * |
| 祝洪艳等: ""五味子超临界萃取部位化学成分研究"", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112043599A (en) * | 2020-09-02 | 2020-12-08 | 广东微纳生物科技有限公司 | Method for preparing uric acid-reducing powder by nanotechnology and processing equipment |
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