CN112752515A - 饲料添加剂 - Google Patents
饲料添加剂 Download PDFInfo
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Abstract
本发明涉及一种用于改善超数排卵处理后得到的胚胎品质的哺乳动物的饲料添加剂，所述饲料添加剂含有甘草提取物；以及一种用于改善超数排卵处理后得到的胚胎品质的方法，其包括使用添加有甘草提取物的饲料饲养经过超数排卵处理的哺乳动物。
Description
技术领域
本发明涉及含有甘草提取物的、用于改善超数排卵处理后得到的胚胎品质的哺乳动物的饲料添加剂；以及包括使用添加有甘草提取物的饲料饲养经过超数排卵处理的哺乳动物的、用于改善超数排卵处理后得到的胚胎品质的方法。
背景技术
为了有效地进行哺乳动物的改良、增殖，广泛进行受精卵移植。体内受精卵适于这种移植用的受精卵。因此，为了由经过受精的雌的哺乳动物回收体内受精卵，进行超数排卵处理。超数排卵处理通过将促卵泡激素以一天2次、3～5天渐减给予雌的哺乳动物来进行。但是，根据雌的哺乳动物的健康状态等，存在由该处理后得到的受精卵产生正常胚胎的频率有可能降低这种问题。另外，为了改善由通过超数排卵处理得到的受精卵的正常胚胎产生率，已知在处理前注射雌二醇等雌性激素的方法。但是，注射这种需要通过兽医进行的处置，对于奶农而言会成为负担，因此寻求对于繁殖农民而言能够自己实施的手段。
发明内容
发明要解决的问题
本发明的目的在于，提供实现超数排卵处理后得到的胚胎品质的改善的哺乳动物用饲料添加剂或方法。
用于解决问题的方案
本发明人等为了解决前述问题而反复深入研究，结果发现，通过将甘草提取物添加到哺乳动物用饲料中，能够改善摄取了该饲料的哺乳动物的超数排卵处理后得到的胚胎品质，基于这种发现完成了本发明。即，本发明如以下所述。
[1]一种用于改善超数排卵处理后得到的胚胎品质的哺乳动物的饲料添加剂，所述饲料添加剂含有甘草提取物。
[2]根据[1]所述的饲料添加剂，其中，哺乳动物为牛。
[3]一种用于改善超数排卵处理后得到的胚胎品质的方法，其包括使用添加有甘草提取物的饲料饲养经过超数排卵处理的哺乳动物。
[4]根据[3]所述的方法，其中，哺乳动物为牛。
[5]根据[4]所述的方法，其中，以20g/天/头的用量给予甘草提取物。
[6]根据[5]所述的方法，其中，给予甘草提取物60天以上。
发明的效果
根据本发明，可以改善超数排卵处理后得到的胚胎品质。
具体实施方式
本发明中，甘草指的是属于甘草属(Glycyrrhiza)的植物。甘草属为在地中海地区、小亚细亚、俄罗斯南部、中亚细亚、中国北部、北美洲等野生的豆科的多年生草本植物。本发明中，可以使用例如：刺甘草(Glycyrrhiza acanthocarpa)、粗毛甘草(G.aspera)、直甘草(G.astragalina)、白花甘草(G.bucharica)、刺毛甘草(G.echinata)(俄罗斯甘草)、无腺毛甘草(G.eglandulosa)、臭甘草(G.foetida)、旱地甘草(G.foetidissima)、光果甘草(G.glabra)(西班牙甘草)、假甘草(G.gontscharovii)、管甘草(G.iconica)、胀果甘草(G.inflate)、黄甘草(G.korshinskyi)、北美甘草(G.lepidota)(美国甘草)、刺果甘草(G.pallidiflora)、圆果甘草(G.squamulosa)、三叶甘草(G.triphylla)、乌拉尔甘草(G.uralensis)、云南甘草(G.yunnanensis)、新疆甘草(G.inflata)。它们可以使用1种或组合使用2种以上。
本发明中使用的甘草提取物可以将植物体的根和/或茎适当加工来使用。本发明中，可以使用市售品，另外也可以使用利用该领域中公知的方法制造的甘草提取物。本发明中使用的甘草提取物的甘草酸的含量为2.5％以上、优选5％以上、更优选10％以上、进一步优选13％以上。
对于甘草提取物的提取溶剂没有特别限定，例如可以使用水、醇(例如甲醇、乙醇、正丙醇、正丁醇等)、丙酮、乙酸乙酯等。另外，得到其干燥粉末的情况下，例如可以通过利用减压干燥、喷雾干燥等本领域技术人员通常使用的方法去除提取物的溶剂来得到。
超数排卵处理指的是例如对于牛而言，通过激素处理使通常1次仅排卵1个的卵子排卵几个，进一步进行人工授精，由此在体内产生多个受精卵，向子宫内流通液体将这些受精卵回收的技术。
本发明中，“改善超数排卵处理后得到的胚胎的品质”指的是例如能够移植的胚胎的平均数增加、平均胚胎品质评分的改善、能够移植的胚胎的比率增加。
本发明中，哺乳动物指的是例如猴子、狗、猫、山羊、绵羊、猪、豚鼠、兔子、小鼠、大鼠和人，优选为牛。
本发明中，“饲料”指的是适于哺乳动物进行的摄取、或以此为意图的所有的化合物、制备品、混合物、或组合物。本发明中，饲料添加剂指的是添加到为了供于家畜等的营养而使用的饵食(饲料)作为饲料的品质保持、辅助营养而利用的添加剂。
本发明的1个方式为用于改善超数排卵处理后得到的胚胎品质的方法，其包括使用添加有甘草提取物的饲料饲养经过超数排卵处理的哺乳动物、例如牛。
本发明的甘草提取物的给予量，只要得到效果则没有特别限制，为10～40g/天/头、优选15～25g/天/头、更优选20g/天/头。
本发明的甘草提取物的给予期间，只要得到所希望效果则可以为任意的期间，但是优选给予60天以上。
实施例
将具有1～4次的分娩经验、由最后的分娩起至少60天后通过超声波检查而发现具有正常子宫的黑毛日本种(n＝136)用作实验动物。对于处理组(n＝90)的动物，以20g/天/头持续性地给予甘草提取物(Fabric Onishi Co.,Ltd.、具有至少13％的甘草酸含量)60～90天直至回收胚胎为止。对照组(n＝46)由最后的分娩日起直至胚胎回收为止完全不摄取甘草。
对于两组中发情后8～11天进行了超数排卵处理的牛，连续3天(递减用量：5AU×2、3AU×2及2AU×2)、一天2次、早晨和傍晚以肌肉注射给予总量20AU(阿穆尔单位(Armourunit))的促卵泡激素(FSH)。在给予FSH的第3天的早晨和傍晚分别给予前列腺素F2α(Pronalgon F)25mg及15mg。在发情期的开始后12小时及24小时进行人工授精，在发情后第7天使用子宫灌注法回收胚胎。
对于处理组和对照组中的被回收的能够移植的胚胎的卵子/胚胎的平均数、和(基于IETS指南)的平均胚胎品质评分，通过t检验进行比较。使用卡方检验，比较2个组的能够移植的胚胎的比率。
在处理组(21.7±11.8)和对照组(15.8±11.3)之间，卵子/胚胎的平均数没有发现显著性差异，但是前者存在提供更多的卵母细胞的倾向。在处理组(13.1±8.3)和对照组(8.1±5.3)之间，能够移植的胚胎的平均数发现显著性差异(p<0.05)。另外，对于平均胚胎品质评分而言，处理组(1.6±0.4)相对于对照组(2.2±0.6)显著优异。对于能够移植的胚胎的比率而言，处理组(60.3±26.4％)与对照组(51.2±26.0％)相比显著优异(p<0.05)。
这些结果示出，对于黑毛日本种进行60天以上的甘草提取物的饲养，改善超数排卵处理后得到的胚胎的品质，实现每头牛的能够移植的胚胎的平均数的增加。
产业上的可利用性
本发明的饲料添加剂对于改善超数排卵处理后得到的胚胎品质而言有用。
Claims (6)
1.一种用于改善超数排卵处理后得到的胚胎品质的哺乳动物的饲料添加剂，所述饲料添加剂含有甘草提取物。
2.根据权利要求1所述的饲料添加剂，其中，哺乳动物为牛。
3.一种用于改善超数排卵处理后得到的胚胎品质的方法，其包括使用添加有甘草提取物的饲料饲养经过超数排卵处理的哺乳动物。
4.根据权利要求3所述的方法，其中，哺乳动物为牛。
5.根据权利要求4所述的方法，其中，以20g/天/头的用量给予甘草提取物。
6.根据权利要求5所述的方法，其中，给予甘草提取物60天以上。
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| JP2018178878A JP6589102B1 (ja) | 2018-09-25 | 2018-09-25 | 飼料添加剤 |
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| PCT/JP2019/037524 WO2020067125A1 (ja) | 2018-09-25 | 2019-09-25 | 飼料添加剤 |
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| JP2001095502A (ja) * | 1999-10-04 | 2001-04-10 | T Hasegawa Co Ltd | 家畜用飼料添加剤 |
| WO2009041449A1 (ja) * | 2007-09-25 | 2009-04-02 | Kaneka Corporation | 抗肥満剤 |
| JP2011167184A (ja) * | 2010-02-16 | 2011-09-01 | Tamura Yakuso Nojo Group:Kk | 家畜の繁殖障害改善方法 |
| JP5868453B2 (ja) * | 2014-05-15 | 2016-02-24 | コ—キン化学株式会社 | 繁殖母牛における繁殖能の改善剤組成物および改善方法 |
| JP2016013116A (ja) * | 2014-07-03 | 2016-01-28 | 株式会社田村薬草農場グループ | 家畜の飼育方法 |
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| RU2648787C2 (ru) * | 2016-06-22 | 2018-03-28 | Федеральное государственное бюджетное научное учреждение "Дальневосточный научно-исследовательский институт сельского хозяйства" (ФГБНУ "ДВ НИИСХ") | Средство для профилактики бесплодия у коров голштинской породы в период адаптации к муссонному климату |
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| AU2019351354A1 (en) | 2021-03-18 |
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| JP6589102B1 (ja) | 2019-10-16 |
| BR112021005331A2 (pt) | 2021-06-15 |
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