CN112723557B - Composite ecological treating agent for treating blue-green algae and preparation method and application thereof - Google Patents

Composite ecological treating agent for treating blue-green algae and preparation method and application thereof Download PDF

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CN112723557B
CN112723557B CN202011450546.9A CN202011450546A CN112723557B CN 112723557 B CN112723557 B CN 112723557B CN 202011450546 A CN202011450546 A CN 202011450546A CN 112723557 B CN112723557 B CN 112723557B
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treating agent
pond
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CN112723557A (en
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常国亮
潘正军
朱传坤
吴楠
王辉
丁怀宇
徐建明
强晓刚
宣富军
李嘉尧
成永旭
吴旭干
潘建林
唐建清
付龙龙
陈友明
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Huaiyin Normal University
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

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  • Biodiversity & Conservation Biology (AREA)
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  • Environmental & Geological Engineering (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a composite ecological treating agent for treating blue-green algae, a preparation method and application thereof, relating to the technical field of aquaculture; the composite ecological treating agent consists of composite bacillus, zeolite powder, water-sprinkling detoxifying vitamin C and granular oxygen in a weight ratio of (0.9-1.4) to (9-11) to (0.4-1) to 1; grinding the granular oxygen, and then mixing the ground granular oxygen with a uniform mixture of bacillus compositus, zeolite powder and water-splashing detoxifying vitamin C to prepare a composite ecological treating agent for treating blue algae; the composite ecological treating agent is sprayed into the culture pond with blue algae, so that the blue algae can be prevented and controlled by an ecological method through nutrition competition, the hypoxia of the pond culture water body caused by the death of algae after the blue algae is treated is avoided, and good conditions are created for the survival and growth of river crabs and crayfish.

Description

Composite ecological treating agent for treating blue-green algae and preparation method and application thereof
Technical Field
The invention relates to the technical field of aquaculture, in particular to a composite ecological treating agent for treating blue-green algae and a preparation method and application thereof.
Background
River crabs and crayfishes are main characteristic aquatic products in Jiangsu province, and in 2019, the annual output of the river crabs and the crayfishes respectively reaches 36.48 ten thousand tons and 20.44 ten thousand tons. In the process of culturing river crabs and crayfishes, good water quality is a necessary condition for the healthy growth of the river crabs and the crayfishes. In recent years, due to the reasons that weather or waterweeds are poorly managed, a large amount of icefish is fed, and the like, blue algae frequently explode in the river crab and crayfish culture pond in 6-9 months at a high temperature. Blue algae can be quickly propagated and can be distributed in the whole pond within 2-3 days at the fastest speed, and when dissolved oxygen in water is greatly consumed by the blue algae or weather mutation occurs at night, a large amount of algae can die due to insufficient oxygen. When the blue algae die, a large amount of toxic and harmful substances such as hydroxylamine, hydrogen sulfide and the like are generated, and the substances are accumulated in water to cause fish poisoning and death, so that the survival rate of shrimps and crabs is reduced, the culture yield is reduced, and great economic loss is caused to farmers.
Currently, methods for killing blue algae in the existing records include drainage methods, mechanical collection and removal methods and drug killing methods. The drainage method is characterized in that by means of the characteristic that blue-green algae like strong light, 30-40 cm of surface water body is drained in the middle of a fine day, and the quantity of the blue-green algae in the pool water can be effectively reduced. The drainage method has the advantages that blue algae in the pond can be quickly drained without influencing the survival of river crabs and crayfish; the disadvantage is that the blue algae can not be killed thoroughly, and the blue algae is easy to roll up the soil to cover the whole pond in a high-temperature sunny day. The mechanical collecting and removing method is to collect the blue algae in the surface water body by using mechanical equipment and remove the blue algae from the pond. The mechanical collection and removal method consumes a lot of manpower and material resources, and is rarely used at present. The medicine killing method is to kill blue algae with halogen bleaching powder, strong chlorine, chlorine dioxide, bromochlorohydantoin, heavy metal salt copper sulfate, strong oxidant potassium permanganate, etc. The method has the advantages of time and labor saving, and thorough blue algae killing, and has the defects that the medicaments stimulate the growth of river crabs and crayfishes to influence the growth of the river crabs and the crayfishes, and the river crabs and the crayfishes die due to improper operation even oxygen deficiency and other factors.
Disclosure of Invention
The invention aims to provide a composite ecological treating agent for treating blue-green algae, a preparation method and an application thereof, which are used for solving the technical problems that the blue-green algae cannot be thoroughly killed when the blue-green algae is exploded in a culture pond and the influence on culture aquatic products is large, protecting the ecological environment of culture water areas while preventing and controlling the blue-green algae, avoiding the hypoxia of pond culture water bodies caused by the death of algae after the blue-green algae is treated, and creating good conditions for the survival and the growth of the culture aquatic products such as river crabs, crayfish and the like.
In order to achieve the purpose, the invention provides the following scheme:
one of the purposes of the invention is to provide a composite ecological treating agent for treating blue algae, which consists of composite bacillus, zeolite powder, water-splashing detoxifying vitamin C and particle oxygen in a weight ratio of (0.9-1.4) to (9-11) to (0.4-1) 1.
The invention also aims to provide a preparation method of the composite ecological treating agent for treating the blue algae, which comprises the following steps:
the method comprises the following steps: preparing bacillus compositus, zeolite powder, water-splashing detoxifying vitamin C and granular oxygen according to the weight ratio;
step two: firstly, uniformly mixing the bacillus compositus, the zeolite powder and the water-splashing detoxifying vitamin C to obtain a uniform mixture, then crushing the granular oxygen and mixing the crushed granular oxygen into the uniform mixture to prepare the composite ecological treating agent for treating the blue algae.
The invention also aims to provide a method for treating blue algae by using the composite ecological treating agent for treating blue algae, which comprises the following steps:
the method comprises the following steps: when the blue-green algae in the pond are in a peak period or an aging period, discharging pond water floating with an oil film at the leeward part of the pond;
step two: and spraying the composite ecological treating agent.
The fourth purpose of the invention is to provide a method for treating blue algae by using the composite ecological treating agent for treating blue algae, wherein the composite ecological treating agent is sprayed when the blue algae in the pond is in a growing period.
Furthermore, the method for treating the blue algae is carried out in the daytime and in the sunny weather.
Furthermore, the position of spraying the composite ecological treatment agent is 1/3 areas in the downwind direction of the pond.
Further, the time for spraying the composite ecological treatment agent is 6: 00-6: 30.
further, the interval between the first step and the second step is less than 24 hours.
Furthermore, the dosage of the composite ecological treating agent per mu is 5.06-6.08 kg.
The invention discloses the following technical effects:
the composite ecological treating agent prepared by the invention has the advantages of easily available raw materials, economy, environmental protection and convenient preparation; the compound bacillus can rapidly degrade organic pollutants in water, and compete with the blue algae for nutrition to control the growth of the blue algae; the zeolite powder is used as a carrier of the bacillus, so that the thalli are uniformly sprayed in the water body of the pond, and the zeolite powder is helpful for removing ammonia nitrogen in water, purifying water quality and relieving the water transfer phenomenon; vitamin C can enhance the organism vitality and disease resistance of aquatic animals, and reduce the stress reaction of crayfish and river crab (river crab stress reaction means that after the river crab encounters a stress source, the river crab is identified and stimulated by a nervous system, and then a series of biological reactions are initiated to the tissues for defense, which is usually shown as abnormal activities, reduced ingestion and the like of the river crab caused by environmental changes); the granular oxygen can quickly improve the dissolved oxygen amount of the bottom layer of the pond, create a good pond bottom environment, effectively prevent oxygen deficiency caused by bacillus proliferation, decomposition of dead blue algae at the bottom of the pond and other reasons, and prevent shrimps and crabs from oxygen deficiency death; the application method of the composite ecological treatment agent avoids the low dissolved oxygen period of the pond before six points in the morning, and effectively prevents the water body of the pond from being anoxic; the floating period of half blue algae in the pond water body from six to six points in the morning is selected, and the blue algae is pressed to the bottom of the pond for treatment by applying the composite ecological treating agent, so that the blue algae prevention and control effect is obviously improved. The area of the spraying position selection pond 1/3 is used for improving the concentration of the composite ecological treating agent in a treatment area, improving the blue algae treatment effect, leaving a safe activity area at the bottom of the pond for river crabs and reducing the stimulation of the river crabs caused by blue algae treatment.
The composite ecological treating agent is prepared by the composite bacillus, the zeolite powder, the water-splashing detoxifying vitamin C and the granular oxygen according to a certain weight ratio, is convenient to operate, consumes less manpower and material resources, has synergistic effect among the components, achieves the effect of effectively preventing and controlling the blue-green algae, protects the ecological environment of a culture water area, avoids the hypoxia of a pond culture water body caused by the death of algae after the blue-green algae is treated, and creates good conditions for the survival and the growth of culture aquatic products such as river crabs, crayfish and the like.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
Example 1:
1. according to the 20 mu area of the river crab culture pond with the blue algae outbreak, 4kg of composite bacillus, 40kg of zeolite powder, 4kg of water-splashing detoxifying vitamin C2kg and 4kg of particle oxygen are prepared in advance according to the weight ratio of the composite bacillus, the zeolite powder, the water-splashing detoxifying vitamin C and the particle oxygen for standby.
2. Selecting continuous sunny days, and when the blue-green algae in the pond are in the growing period, 5: 10, taking each prepared raw material, firstly uniformly stirring the bacillus compositus, the zeolite powder and the water-splashing detoxifying vitamin C, then crushing the particle oxygen, and then mixing the crushed particle oxygen with the uniformly mixed substance to prepare the composite ecological treating agent.
3. 6 in the morning: 00, spraying the composite ecological treating agent into the river crab culture pond with the blue algae erupting in the downwind direction of 1/3 areas.
The initial chlorophyll a concentration of the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 1:
TABLE 1
Figure BDA0002826654450000051
Example 2:
1. according to the area of 30 mu of a crayfish culture pond with blue algae, 6.75kg of composite bacillus, 60kg of zeolite powder, 3.75kg of water-sprinkling detoxification vitamin and 6kg of particle oxygen are prepared in advance according to the weight ratio of the composite bacillus, the zeolite powder, the water-sprinkling detoxification vitamin C and the particle oxygen for standby.
2. And (3) selecting continuous sunny days, wherein the blue algae in the crayfish aquaculture pond are in the peak period, and discharging pond water floating with an oil film at the leeward part of the aquaculture pond in the day 1.
3. Morning day 2 5: 00, taking each prepared raw material, firstly uniformly stirring the bacillus compositus, the zeolite powder and the water-splashing detoxifying vitamin C, then crushing the particle oxygen, and then mixing the crushed particle oxygen with the uniformly mixed substance to prepare the composite ecological treating agent.
4. 6 in the morning: 15, spraying the composite ecological treating agent into the crayfish culture pond with the blue algae in the downwind direction 1/3 area.
The initial chlorophyll a concentration of the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 2:
TABLE 2
Figure BDA0002826654450000061
Example 3:
1. according to the area of 40 mu of a river crab and crawfish mixed culture pond with blue algae outbreak, 10kg of bacillus compositus, 80kg of zeolite powder, 6kg of water-sprinkling detoxification vitamin and 8kg of particle oxygen are prepared in advance according to the weight ratio of the bacillus compositus, the zeolite powder, the water-sprinkling detoxification vitamin C and the particle oxygen for standby.
2. And (3) selecting continuous sunny days, wherein the blue algae in the river crab and crayfish mixed culture pond are in the aging period, and discharging pond water with an oil film floating at the downwind part of the culture pond in the day 1.
3. Day 2, morning 4: 50, taking each prepared raw material, firstly uniformly stirring the bacillus compositus, the zeolite powder and the water-splashing detoxifying vitamin C, then crushing the particle oxygen, and then mixing the crushed particle oxygen with the uniformly mixed substance to prepare the composite ecological treating agent.
4. 6 in the morning: 30, spraying the composite ecological treating agent into the downwind direction 1/3 area of the river crab and crawfish mixed culture pond with the blue algae.
The initial chlorophyll a concentration of the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 3:
TABLE 3
Figure BDA0002826654450000062
Figure BDA0002826654450000071
Comparative example 1:
the difference from the example 1 is that the complex ecological treating agent does not contain water-repellent detoxified vitamin C.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 4:
TABLE 4
Figure BDA0002826654450000072
Comparative example 2:
the difference from the example 1 is that the composite ecological treating agent does not contain granular oxygen.
The initial chlorophyll a concentration of the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 5:
TABLE 5
Figure BDA0002826654450000073
Comparative example 3:
the difference from example 1 is that the amount of Bacillus compositus added is 8 kg.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 6:
TABLE 6
Figure BDA0002826654450000074
Figure BDA0002826654450000081
Comparative example 4:
the difference from the example 1 is that the time for spraying the composite ecological treating agent in the step 3 is 5:30 in the morning.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 7:
TABLE 7
Figure BDA0002826654450000082
Comparative example 5:
the difference from the example 1 is that the time for spraying the composite ecological treating agent in the step 3 is 7:00 in the morning.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 8:
TABLE 8
Figure BDA0002826654450000083
Comparative example 6:
the difference from the example 1 is that the position of spraying the composite ecological treatment agent in the step 3 is the area 1/4 under the pond.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 9:
TABLE 9
Figure BDA0002826654450000091
Comparative example 7:
the difference from the example 1 is that the position of spraying the composite ecological treatment agent in the step 3 is the area 1/2 under the pond.
The initial chlorophyll a concentration in the pond and the chlorophyll a concentrations in the pond water were measured at 3 rd, 5 th and 7 th days after spraying the composite ecological treatment agent, and the results are shown in table 10:
watch 10
Figure BDA0002826654450000092
In examples 1 to 3 and comparative examples 1 to 7, the growth of shrimps and crabs in the pond after 7 days of application of the composite ecological treating agent is shown in Table 8:
TABLE 11
Figure BDA0002826654450000093
Figure BDA0002826654450000101
As can be seen from tables 1 to 11, in examples 1 to 3, after the composite ecological treatment agent is applied for 7 days, blue algae are effectively controlled, the culture of shrimps and crabs in the pond is not affected, and the shrimps and crabs grow well; in comparative examples 1-2, after 7 days of application of the composite ecological treatment agent containing no splash-water detoxified vitamin C or granular oxygen, the concentration of blue algae was reduced but not effectively controlled, the stress response of river crabs was obvious, and the ingestion was reduced; in the comparative example 3, after the composite ecological treating agent with the adjusted weight ratio of the components is applied, the pond has slight oxygen deficiency, the cost of preventing and controlling blue algae is increased, and river crabs have slight emergency reaction; in comparative examples 4-5, the blue algae concentration was reduced but not effectively controlled before the sprinkling time of the composite ecological treatment agent was adjusted to 6:00 and 6:30 in the morning, the pond had a slight anaerobic phenomenon, and river crabs had a slight emergency reaction, because the sprinkling time of the composite ecological treatment agent was too early, the water in the pond was in a hypoxic period, and the blue algae had floated to the surface of the water after the sprinkling time was too late, which resulted in this situation; in comparative examples 6 to 7, the blue algae were controlled by adjusting the positions of spraying the composite ecological treating agent to 1/4 area and 1/2 area in the downwind direction of the pond, but the blue algae treatment effect was reduced as compared with example 1 because the area of application of the composite ecological treating agent was too large or too small.
The bacillus compositus, the zeolite powder, the water-splashing detoxifying vitamin C and the granular oxygen are all purchased from the market.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (6)

1. A method for treating blue algae by using a composite ecological treating agent is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: when the blue algae in the pond is in a peak period or an aging period, discharging pond water floating with an oil film at the leeward part of the pond;
step two: spraying the composite ecological treating agent;
the composite ecological treating agent consists of composite bacillus, zeolite powder, water-sprinkling detoxifying vitamin C and granular oxygen in a weight ratio of (0.9-1.4) to (9-11) to (0.4-1) to 1;
the position of the composite ecological treating agent sprayed into the pond is 1/3 area in the downwind direction of the pond;
the time for spraying the composite ecological treating agent is 6: 00-6: 30.
2. a method for treating blue algae by using a composite ecological treating agent is characterized by comprising the following steps: spraying the composite ecological treating agent of claim 1 when the blue-green algae in the pond are in the growth period;
the position of the composite ecological treating agent sprayed into the pond is 1/3 area in the downwind direction of the pond;
the time for spraying the composite ecological treating agent is 6: 00-6: 30.
3. the method of processing cyanobacteria according to claim 1, wherein: the preparation method of the composite treating agent comprises the following steps:
the method comprises the following steps: preparing bacillus compositus, zeolite powder, water-splashing detoxifying vitamin C and granular oxygen according to the weight ratio;
step two: firstly, uniformly mixing the bacillus compositus, the zeolite powder and the water-splashing detoxifying vitamin C to obtain a uniform mixture, then crushing the granular oxygen and mixing the crushed granular oxygen into the uniform mixture to prepare the composite ecological treating agent for treating the blue algae.
4. The method for processing cyanobacteria according to any of claims 1 or 2, wherein: the method for treating the blue algae is carried out in the daytime and in the sunny weather.
5. The method of processing cyanobacteria according to claim 1, wherein: the time interval between the first step and the second step is less than 24 hours.
6. The method for processing cyanobacteria according to any of claims 1 or 2, wherein: the dosage of the composite ecological treating agent per mu is 5.06-6.08 kg.
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