CN112707515A - Water body repairing agent for reducing water body N, P and preparation method and application thereof - Google Patents
Water body repairing agent for reducing water body N, P and preparation method and application thereof Download PDFInfo
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- CN112707515A CN112707515A CN202011539947.1A CN202011539947A CN112707515A CN 112707515 A CN112707515 A CN 112707515A CN 202011539947 A CN202011539947 A CN 202011539947A CN 112707515 A CN112707515 A CN 112707515A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 127
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 42
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 38
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 108010059892 Cellulase Proteins 0.000 claims abstract description 15
- 229920002261 Corn starch Polymers 0.000 claims abstract description 15
- 229960000892 attapulgite Drugs 0.000 claims abstract description 15
- 229940106157 cellulase Drugs 0.000 claims abstract description 15
- 239000008120 corn starch Substances 0.000 claims abstract description 15
- 229910052625 palygorskite Inorganic materials 0.000 claims abstract description 15
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 14
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 14
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 13
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 13
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 13
- 239000002131 composite material Substances 0.000 claims abstract description 13
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims abstract description 8
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 3
- 239000002994 raw material Substances 0.000 claims description 27
- 239000002068 microbial inoculum Substances 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 238000009360 aquaculture Methods 0.000 claims description 10
- 244000144974 aquaculture Species 0.000 claims description 10
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 7
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 7
- 238000005067 remediation Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 abstract description 12
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000008263 repair mechanism Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- -1 compound sodium nitrophenolate Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a water body repairing agent for reducing N, P in a water body, a preparation method and an application thereof, and the water body repairing agent comprises a composite microbial agent consisting of enterococcus faecalis, bacillus coagulans, bacillus licheniformis, saccharomycetes, cellulase and phosphatase, corn starch and attapulgite. The preparation method of the repairing agent comprises the following steps: step 1: adding attapulgite into corn starch according to a certain proportion, and sequentially adding various bacteria and enzymes according to a certain proportion; step 2: stirring for 20 minutes at constant speed by using a V-shaped stirrer or a three-V stirrer; and step 3: and mixing the water with pond water according to the proportion of 1: 100-1: 400, and uniformly stirring to obtain the water body repairing agent for reducing the water body N, P, and a preparation method and application thereof. The invention has sufficient effective bacteria content and stabilizes the ph of the water body. The problem of the water body beneficial bacterium content is not enough is solved. Reducing water ammonia nitrogen, reducing harmful indexes such as hydrogen sulfide and the like, decomposing water organic matters and residual baits and providing a high-quality ecological environment for the cultured objects.
Description
Technical Field
The invention relates to a water body repairing agent for reducing N, P water body, a preparation method and application thereof, belonging to the technical field of aquaculture.
Background
Due to the phenomena of large amount of medicine application and large amount of water change, the aquaculture environment in China is increasingly worsened, and in recent years, the red tide phenomenon frequently occurs in China, so that not only is the ecological balance disturbed and the diseases are frequently caused, but also huge economic loss is brought. The use of ecological remediation techniques for treating aquaculture wastewater has received wide acceptance, including physical remediation, chemical remediation and biological remediation. Compared with the traditional physical and chemical remediation, the biological remediation has the advantages of low cost, short time consumption, thorough purification, difficult generation of secondary pollution, no harm to the breeding function, no damage to ecological balance and the like. In the ecological restoration technology, the combination purification of the sediment environment and the water quality environment by using the beneficial bacteria and algae preparation is a brand-new attempt, not only can the environment be improved, but also the algae can provide aquatic food. However, the specific repair mechanisms such as the symbiotic mechanism of bacteria and algae, the bacteriophagophy, the physiological and biochemical pathway and the ecological combination, and the avoidance and antagonism of environmental influence factors need to be further understood. Only by mastering the details of the repair mechanism, the repair technology can be effectively improved and the repair monitoring can be effectively carried out. In addition, the construction of genetically engineered bacteria and algae transfers the bacteria and algae gene with excellent repairing efficiency into other bacteria and algae or plants, breaks through the limitation of species isolation, can construct the repairing organisms which are most suitable for the ecological system and have the most comprehensive functions aiming at different degradation reasons of different aquatic product ecological systems, can also construct the repairing organisms with stronger environmental adaptability and wider activity maintaining range, and realizes the repairing function through effective immobilization technology and carriers.
Some of the existing repairing agents enable a water body to be clear and transparent, and some of the existing repairing agents enable the water body to be turbid and have no vitality. The effective bacteria content of the product is seriously insufficient or the target substance is not in accordance with the actual filling material. The purpose of solving the harmful indexes (nitrite, ammonia nitrogen and hydrogen sulfide) of the aquaculture water body can not be achieved. The problem that organic matters in the water body and residual baits fed manually damage the water body can not be solved.
Disclosure of Invention
In order to solve the technical problems, the invention provides a water body restoration agent for reducing water body N, P and preparation and application thereof, and the specific technical scheme is as follows:
a water body restoration agent for reducing N, P, which is characterized in that: the raw material formula comprises: the microbial inoculum comprises a composite microbial inoculum consisting of enterococcus faecalis, bacillus coagulans, bacillus licheniformis and saccharomycetes, and the distribution density of each component in the composite microbial inoculum is as follows:
further, the composite microbial inoculum comprises cellulase and phosphatase, the composite microbial inoculum is mixed with the cellulase and the phosphatase to form a mixed preparation, and the mass ratio of the composite microbial inoculum to the mixed preparation is as follows: 1: 1.589-1: 1.536.
Further, the mass ratio of the cellulase to the phosphatase is as follows: 1: 0.688-1: 0.607.
Further, the corn starch and the attapulgite clay are also included, and the mass ratio of the mixed preparation to the mixture of the corn starch and the attapulgite clay is as follows: 57: 1000-84.5: 1000.
Further, the mass ratio of the corn starch to the attapulgite is as follows: 1: 0.257-1: 0.065.
Further, the distribution density in the composite microbial inoculum is as follows:
the preparation method of the water body repairing agent for reducing the water body N, P is characterized by comprising the following steps: the method comprises the following steps:
step 1: adding attapulgite into corn starch according to the formula of claim 1, and sequentially adding enterococcus faecalis, bacillus coagulans, bacillus licheniformis, yeast, cellulase and phosphatase according to the formula of claim 1 to obtain a mixture a;
step 2: uniformly stirring the mixture a for 20 minutes by using a V-shaped stirrer or a three-V stirrer to obtain a mixture b;
and step 3: and (3) mixing the mixture b with pond water in a ratio of 1: 100-1: 400, and uniformly stirring to obtain the water body repairing agent for reducing the water body N, P.
The application of the water body repairing agent for reducing the water body N, P is characterized in that: the water restoration agent for reducing the N, P content in the water is sprayed to the aquaculture water at a concentration of 33.67-200.5 kg/mu, and the average depth of the water is 0.5-1.5 m.
Further, the water body repairing agent for reducing the water body N, P is sprinkled on the aquaculture water body at the concentration of 50.5 kg/mu, and the water body depth is 1m on average.
The invention has the beneficial effects that: the effective bacteria content is sufficient, and the PH of the water body is stabilized. The problem of the water body beneficial bacterium content is not enough is solved. Reducing water ammonia nitrogen, reducing harmful indexes such as hydrogen sulfide and the like, decomposing water organic matters and residual baits and providing a high-quality ecological environment for the cultured objects.
Detailed Description
In the invention, the unit of hundred million/gram refers to how many hundred million bacteria are contained in the mixture a of the water body restoration agent for reducing N, P in a water body per gram, for example, enterococcus faecalis is 2.5-3.75 hundred million/gram, namely, the mixture a of the water body restoration agent for reducing N, P in a water body per gram contains 2.5-3.75 hundred million lactobacillus acidophilus.
The following specific examples are provided to verify the technical effects achieved by the present invention:
each pond is a pond for growing over-mature shrimps and crabs, the water quality conditions are basically the same, and each pond is respectively treated by the following different treatments:
blank comparative example: 2 mu of pond water with the average water depth of 1m without adding any preparation.
Control group: adding a common repairing agent which contains sodium humate, amino acid fertilizer water powder and compound sodium nitrophenolate, mixing with water according to the mass ratio of 1:200, and then adding 2 mu of pond water with the average depth of 1m under the same condition.
Example 1: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1000g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1000g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
1200g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
4000g of yeast with the raw material content of 200 hundred million/g,
2600g of cellulase, 1600g of phosphatase,
150kg of corn starch and 38.6kg of attapulgite.
Example 2: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1100g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1100g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
1200g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
4400g of raw material of 200 hundred million/g of yeast,
2800g cellulase, 1800g phosphatase,
155kg of corn starch and 32.6kg of attapulgite.
Example 3: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1200g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1200g of bacillus coagulans with the raw material content of 1000 hundred million/g,
1400g of raw material of the bacillus licheniformis with the content of 1000 hundred million/g,
4800g of yeast with a raw material content of 200 hundred million/g,
3000g cellulase, 2000g phosphatase
160kg of corn starch and 26.4kg of attapulgite.
Example 4: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1300g of raw material of enterococcus faecalis with the content of 500 hundred million/g,
1300g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
1600g of bacillus licheniformis with the raw material content of 1000 hundred million/g,
5200g of yeast raw material with the content of 200 hundred million/g,
3200g of cellulase, 2200g of phosphatase,
155kg of corn starch and 30.2kg of attapulgite.
Example 5: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1400g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1400g of raw material of bacillus coagulans with the content of 1000 hundred million/g,
1800g of raw material of bacillus licheniformis with the content of 1000 hundred million/g,
5600g of yeast with 200 hundred million/g raw material content,
3400g of cellulase, 2200g of phosphatase,
160kg of corn starch and 24.2kg of attapulgite.
Example 6: the water body repairing agent added into pond water and used for reducing the water body N, P comprises the following components in percentage by weight:
1500g of enterococcus faecalis with the raw material content of 500 hundred million/g,
1500g of bacillus coagulans with the raw material content of 1000 hundred million/g,
2000g of bacillus licheniformis with the raw material content of 1000 hundred million/g,
6000g of yeast with the raw material content of 200 hundred million/gram,
3500g of cellulase, 2400g of phosphatase, and,
172kg of corn starch and 11.1kg of attapulgite.
The following are the pond water quality conditions of the examples, as shown in tables 1-2 below:
TABLE 1
TABLE 2
As can be seen from tables 1 and 2, when the water body repairing agent for reducing the water body N, P according to the present invention was not used, the transparency of the water body in the blank comparative example was 10 to 20cm, and the water quality was turbid and dull. The PH value of the water body is up to 9.3, the dissolved oxygen content of the water body is lower and is 4.3mg/L, the ammonia nitrogen content of the water body is 0.04mg/L, the nitrite content of the water body is 0.29ppm, and the organic matter content is up to 40 g; after the treatment by the common repairing agent, the transparency and the dissolved oxygen are slightly increased in the comparative example, and the PH, the ammonia nitrogen content, the nitrite content and the organic matter content of the water body are slightly reduced; after the water body repairing agent for reducing the water body N, P is treated, the transparency of the water body is 31-35cm, the water body is obviously clear, the water quality is clear and glossy, the pH of the water body is reduced to about 8.2, which is obviously lower than that of a blank comparative example, the dissolved oxygen content of the water body is increased to 6.8mg/L, the ammonia nitrogen content of the water body is reduced to below 0.02mg/L, the nitrite content of the water body is reduced to below 0.1ppm, and the organic matter content is obviously reduced to 7 g. Generally, in aquaculture, the pH of a water body which is most beneficial to the growth of aquatic organisms is about 8.2, the content of ammonia nitrogen is below 0.02mg/L, and the content of nitrite is below 0.1ppm, and the water body repairing agent for reducing N, P in the water body basically conforms to the water body. Researches show that the water restoration agent for reducing the water N, P has a good effect of adjusting water quality, obviously reduces the content of ammonia nitrogen and nitrite in the water, enables the pH of the water to reach a proper value, improves the water quality, and has a good application prospect in aquaculture.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (9)
1. A water body restoration agent for reducing N, P, which is characterized in that: the raw material formula comprises: the microbial inoculum comprises a composite microbial inoculum consisting of enterococcus faecalis, bacillus coagulans, bacillus licheniformis and saccharomycetes, and the distribution density of each component in the composite microbial inoculum is as follows:
2.5-3.75 hundred million/g enterococcus faecalis,
5-7.5 hundred million/g of Bacillus coagulans,
6-10 hundred million/g of Bacillus licheniformis,
4-6 hundred million/g of yeast.
2. The water restoration agent for lowering N, P water according to claim 1, wherein: the composite microbial inoculum comprises cellulase and phosphatase, the composite microbial inoculum is mixed with the cellulase and the phosphatase to form a mixed preparation, and the mass ratio of the composite microbial inoculum to the mixed preparation is as follows: 1: 1.589-1: 1.536.
3. The water restoration agent for lowering N, P water according to claim 2, wherein: the mass ratio of the cellulase to the phosphatase is as follows: 1: 0.688-1: 0.607.
4. The water restoration agent for lowering N, P water according to claim 3, wherein: the corn starch and the attapulgite mixed preparation are prepared from the following raw materials in parts by mass: 57: 1000-84.5: 1000.
5. The water restoration agent for lowering N, P water according to claim 4, wherein: the mass ratio of the corn starch to the attapulgite is as follows: 1: 0.257-1: 0.065.
6. The water restoration agent for lowering N, P water according to claim 1, wherein: wherein the distribution density in the composite microbial inoculum is as follows:
3 hundred million/g enterococcus faecalis,
Bacillus coagulans 6 hundred million/gram,
8 hundred million/g of Bacillus licheniformis,
Yeast 5 hundred million/gram.
7. The method of claim 5, wherein the water N, P is lowered by the method of: the method comprises the following steps:
step 1: adding attapulgite into corn starch according to the formula of claim 1, and sequentially adding enterococcus faecalis, bacillus coagulans, bacillus licheniformis, yeast, cellulase and phosphatase according to the formula of claim 1 to obtain a mixture a;
step 2: uniformly stirring the mixture a for 20 minutes by using a V-shaped stirrer or a three-V stirrer to obtain a mixture b;
and step 3: and (3) mixing the mixture b and pond water in a ratio of 1: 100-1: 400, and uniformly stirring to obtain the water body repairing agent for reducing the water body N, P, and a preparation method and application thereof.
8. The use of the water restoration agent prepared according to claim 7 for lowering N, P in a body of water, wherein: the water restoration agent for reducing the N, P content in the water is sprayed to the aquaculture water at a concentration of 33.67-200.5 kg/mu, and the average depth of the water is 0.5-1.5 m.
9. The use of a water remediation agent according to claim 8 to reduce the level of N, P, wherein: the water body repairing agent for reducing the water body N, P is sprinkled on the aquaculture water body at the concentration of 50.5 kg/mu, and the average depth of the water body is 1 m.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113897311A (en) * | 2021-09-30 | 2022-01-07 | 武汉华扬天乐生物科技有限公司 | Self-culture composite bacteria and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101323843A (en) * | 2008-07-25 | 2008-12-17 | 浙江大学 | Composite bacillus preparation for improving marine culture water environment and preparation thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN103224886A (en) * | 2013-04-24 | 2013-07-31 | 武汉市天辰生物科技有限公司 | Composite microbe inocula for aquatic product culture and application thereof |
| CN108587956A (en) * | 2018-04-25 | 2018-09-28 | 正丰源生物科技（苏州）有限公司 | A kind of composite bacterial fertilizer for sewage disposal |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101323843A (en) * | 2008-07-25 | 2008-12-17 | 浙江大学 | Composite bacillus preparation for improving marine culture water environment and preparation thereof |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN103224886A (en) * | 2013-04-24 | 2013-07-31 | 武汉市天辰生物科技有限公司 | Composite microbe inocula for aquatic product culture and application thereof |
| CN108587956A (en) * | 2018-04-25 | 2018-09-28 | 正丰源生物科技（苏州）有限公司 | A kind of composite bacterial fertilizer for sewage disposal |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113897311A (en) * | 2021-09-30 | 2022-01-07 | 武汉华扬天乐生物科技有限公司 | Self-culture composite bacteria and preparation method and application thereof |
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Application publication date: 20210427 |