CN112673026A - 突变型单链人凝血因子viii融合蛋白及其制备方法与用途 - Google Patents
突变型单链人凝血因子viii融合蛋白及其制备方法与用途 Download PDFInfo
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Abstract
本发明公开了一种突变型重组单链人凝血因子VIII(FVIII)融合蛋白、其制备方法及用途。该融合蛋白从N端到C端依次包含缺失部分B‑结构域的突变型单链人FVIII、柔性肽接头、至少1个人绒毛膜促性腺激素β亚基羧基末端肽刚性单元和延长半衰期部分(优选人IgG Fc变体)。该融合蛋白具有与重组FVIII类似的生物学活性及延长的体内活性半衰期，以及更好的体外和体内稳定性，从而改善融合蛋白的药代动力学和药效。
Description
PCT国内申请，说明书已公开。
Claims (17)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811123918.XA CN110950964B (zh) | 2018-09-26 | 2018-09-26 | 突变型单链人凝血因子viii融合蛋白及其制备方法与用途 |
| CN201811123918X | 2018-09-26 | ||
| PCT/CN2019/107432 WO2020063562A1 (zh) | 2018-09-26 | 2019-09-24 | 突变型单链人凝血因子viii融合蛋白及其制备方法与用途 |
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|---|---|
| CN112673026A true CN112673026A (zh) | 2021-04-16 |
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| CN202110532012.9A Active CN113105562B (zh) | 2018-09-26 | 2018-09-26 | 突变型单链人凝血因子viii在制备融合蛋白中的应用 |
| CN201980059489.0A Pending CN112673026A (zh) | 2018-09-26 | 2019-09-24 | 突变型单链人凝血因子viii融合蛋白及其制备方法与用途 |
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| CN201811123918.XA Active CN110950964B (zh) | 2018-09-26 | 2018-09-26 | 突变型单链人凝血因子viii融合蛋白及其制备方法与用途 |
| CN202110532012.9A Active CN113105562B (zh) | 2018-09-26 | 2018-09-26 | 突变型单链人凝血因子viii在制备融合蛋白中的应用 |
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| WO (1) | WO2020063562A1 (zh) |
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| CN113461827A (zh) * | 2020-03-31 | 2021-10-01 | 安源医药科技（上海）有限公司 | 高效分离纯化重组人凝血因子VIII Fc融合蛋白的方法 |
| CN111808170A (zh) * | 2020-06-29 | 2020-10-23 | 江苏为真生物医药技术股份有限公司 | 多肽、hla-dr蛋白及其制备方法和应用 |
| WO2023227015A1 (zh) * | 2022-05-25 | 2023-11-30 | 江苏晟斯生物制药有限公司 | 具有延长的半衰期的fviii融合蛋白缀合物及其应用 |
| CN116036244B (zh) * | 2023-02-24 | 2023-09-19 | 北京基科晟斯医药科技有限公司 | 培重组人凝血因子VIII-Fc融合蛋白用于治疗含抑制物的血友病A的用途 |
Citations (3)
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| CN1863556A (zh) * | 2003-06-19 | 2006-11-15 | 泰诺士公司 | 用于治疗凝血相关紊乱的组合物和方法 |
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| CN107787328A (zh) * | 2015-05-22 | 2018-03-09 | 瑞士杰特贝林生物制品重组设备股份公司 | 用于治疗血友病的截短的血管性血友病因子多肽 |
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| US4818679A (en) | 1985-02-19 | 1989-04-04 | The Trustees Of Columbia University In The City Of New York | Method for recovering mutant cells |
| US6103501A (en) * | 1997-11-17 | 2000-08-15 | Washington University | Single chain glycoprotein hormones comprising two β and one α subunits and recombinant production thereof |
| EP2332985A3 (en) | 2004-11-12 | 2012-01-25 | Xencor, Inc. | Fc variants with altered binding to FcRn |
| SI2235059T1 (sl) | 2007-12-26 | 2015-06-30 | Xencor, Inc. | Fc variante s spremenjeno vezjo na fcrn |
| US9493543B2 (en) * | 2010-02-16 | 2016-11-15 | Novo Nordisk A/S | Factor VIII fusion protein |
| CN102311495A (zh) * | 2010-06-30 | 2012-01-11 | 上海同科生物科技有限公司 | 新型重组人凝血因子ⅷ及其生产方法 |
| US20130017997A1 (en) * | 2010-08-19 | 2013-01-17 | Amunix Operating Inc. | Factor VIII Compositions and Methods of Making and Using Same |
| CN104114183A (zh) * | 2011-12-20 | 2014-10-22 | 印第安纳大学研究及科技有限公司 | 用于治疗糖尿病的基于ctp的胰岛素类似物 |
| ES2700583T3 (es) | 2012-01-12 | 2019-02-18 | Bioverativ Therapeutics Inc | Procedimientos para reducir la inmunogenicidad contra el Factor VIII en individuos sometidos a terapia con Factor VIII |
| EP2938636A2 (en) * | 2012-12-28 | 2015-11-04 | AbbVie Inc. | High-throughput system and method for identifying antibodies having specific antigen binding activities |
| CN104693270B (zh) * | 2013-12-10 | 2018-10-16 | 清华大学 | 一种用于融合蛋白的连接肽 |
| BR112016015512B1 (pt) * | 2014-01-10 | 2023-12-19 | Bioverativ Therapeutics Inc | Proteína quimérica, composição farmacêutica e seus usos |
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| CN106279436B (zh) * | 2016-08-19 | 2017-10-31 | 安源医药科技（上海）有限公司 | 活化的人凝血因子vii融合蛋白及其制备方法与用途 |
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-
2018
- 2018-09-26 CN CN201811123918.XA patent/CN110950964B/zh active Active
- 2018-09-26 CN CN202110532012.9A patent/CN113105562B/zh active Active
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2019
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| CN110950964A (zh) | 2020-04-03 |
| CN113105562B (zh) | 2023-12-01 |
| EP3858865A9 (en) | 2024-02-14 |
| EP3858865A4 (en) | 2023-04-19 |
| CN113105562A (zh) | 2021-07-13 |
| US20220033476A1 (en) | 2022-02-03 |
| EP3858865A1 (en) | 2021-08-04 |
| CN110950964B (zh) | 2021-06-18 |
| WO2020063562A1 (zh) | 2020-04-02 |
| BR112021005831A2 (pt) | 2021-07-27 |
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