CN112665957A - Whole blood separation device in reagent strip - Google Patents
Whole blood separation device in reagent strip Download PDFInfo
- Publication number
- CN112665957A CN112665957A CN202110080996.1A CN202110080996A CN112665957A CN 112665957 A CN112665957 A CN 112665957A CN 202110080996 A CN202110080996 A CN 202110080996A CN 112665957 A CN112665957 A CN 112665957A
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- reagent strip
- whole blood
- transmission mechanism
- linear transmission
- mounting
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 54
- 210000004369 blood Anatomy 0.000 title claims abstract description 46
- 239000008280 blood Substances 0.000 title claims abstract description 46
- 238000000926 separation method Methods 0.000 title claims abstract description 22
- 238000001179 sorption measurement Methods 0.000 claims abstract description 23
- 230000005540 biological transmission Effects 0.000 claims abstract description 19
- 230000007246 mechanism Effects 0.000 claims abstract description 19
- 239000011324 bead Substances 0.000 claims abstract description 9
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims description 19
- 238000009434 installation Methods 0.000 claims description 15
- 229920000742 Cotton Polymers 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims 1
- 230000001960 triggered effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 19
- 238000013461 design Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 16
- 238000012545 processing Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000004148 unit process Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a whole blood separation device in a reagent strip, which comprises a fixed bearing unit, a reagent strip clamping unit and a reagent strip separating unit, wherein the upper surface of the fixed bearing unit is provided with at least one mounting groove for clamping the reagent strip; the linear transmission mechanism is arranged at one end part of the fixed bearing unit; and the adsorption component is arranged at the upper part of the linear transmission mechanism, and the linear transmission mechanism drives the adsorption component to return up and down. The invention has the advantages that the layout design of the base and the linear transmission mechanism is small and exquisite in structure, and the device can be integrally fixed on a bedside detection instrument to meet the clinical bedside detection requirement; according to the invention, the linear transmission mechanism is utilized to realize the up-and-down reciprocating of the adsorption assembly, and the magnetic beads containing red blood cells are adsorbed by combining the magnet blocks of the adsorption assembly, so that the rapid separation of the whole blood sample is realized.
Description
Technical Field
The invention relates to a whole blood separation technology, in particular to a whole blood separation device in a reagent strip.
Background
In the field of in vitro diagnosis, reagents packaged by single-person reagent strips are usually added to a patient sample by an in vitro diagnosis device respectively to realize detection of the sample. Before the on-machine detection, the whole blood sample of the patient is usually separated and then subjected to subsequent detection. The separation treatment of whole blood is a key step affecting the subsequent detection, and the separation method commonly used in clinic is to realize the separation of whole blood by using a centrifuge. However, because the sample to be tested in a hospital is huge in quantity, a large and heavy high-throughput centrifuge is often selected clinically, the centrifuge structure is often complicated, the occupied space is large, and the clinical bedside detection (Point-of-care Testing, abbreviated as POCT) requirements cannot be met; and manual intervention is more when a centrifugal machine is adopted for separation. Therefore, how to design a whole blood separation device which has less manual intervention and can meet the clinical bedside detection requirement is a technical problem to be solved by the industry.
Disclosure of Invention
The invention aims to provide a whole blood separation device in a reagent strip, which is small in size, small in occupied space and convenient to install; the device can also be directly installed on a bedside detection instrument, and the bedside detection requirement of a patient is met.
In order to achieve the purpose, the invention adopts the following technical scheme:
the whole blood separating device in the reagent strip comprises
The upper surface of the fixed bearing unit is provided with at least one mounting groove for clamping a reagent strip;
the linear transmission mechanism is arranged at one end part of the fixed bearing unit; and
and the adsorption component is arranged on the upper part of the linear transmission mechanism, and the linear transmission mechanism drives the adsorption component to return up and down, so that the adsorption component adsorbs magnetic beads which adsorb red blood cells in the whole blood sample in the reagent strip.
In a preferred embodiment of the present invention, the whole blood separation device in a reagent strip further comprises a heating unit, and the heating unit is arranged at the bottom of the fixed bearing unit.
In a preferred embodiment of the present invention, the fixed bearing unit is a horizontally disposed base, and the heating unit is a heating plate or heat-insulating cotton disposed at the bottom of the base. More preferably, the heating plate is a silicone heating plate.
In a preferred embodiment of the present invention, a plurality of positioning holes corresponding to the plurality of hole sites of the reagent strip one to one are formed in a wall of the mounting groove, and the positioning holes have a tapered structure. When detecting, the accessible locating hole realizes the quick installation of reagent strip on the mounting groove, guarantees the stability of reagent strip, and the locating hole can also increase the heated area of every hole site, makes the reagent strip be heated evenly, guarantees the incubation effect.
In a preferred embodiment of the present invention, the linear transmission mechanism includes a mounting unit disposed at one end of the fixed bearing unit, the mounting unit is provided with a lifting member driven by a linear power source, and the adsorption assembly is disposed on the lifting member.
In a preferred embodiment of the present invention, the mounting unit includes a horizontal mounting member and a vertical mounting member fixedly connected to an end of the horizontal mounting member, the linear power source is a screw rod stepping motor disposed on the horizontal mounting member, and the lifting member is disposed on a screw rod of the screw rod stepping motor.
In a preferred embodiment of the present invention, the adsorption assembly includes a mounting base fixedly attached to an upper portion of the elevating member and at least one magnet block disposed on an upper portion of the mounting base.
In a preferred embodiment of the present invention, the mounting base has at least one mounting head in the same number as the number of the magnet blocks, and the magnet blocks are disposed above the mounting head.
In a preferred embodiment of the invention, the vertical mounting part is further provided with a guide part, the lifting part reciprocates up and down along the guide part, and the guide part has guiding and supporting functions, so that the motion track of the lifting part is effectively ensured, and the motion stability of the lifting part is improved.
In a preferred embodiment of the present invention, a home position sensor is disposed at a lower portion of the vertical mounting member, and a trigger member is disposed on the lifting member, and the trigger member triggers the home position sensor when moving downward to a home position, so that the lifting member is positioned at the home position to facilitate processing of a whole blood sample in a next reagent strip.
The invention has the advantages that the layout design of the base and the linear transmission mechanism is small and exquisite in structure, and the device can be integrally fixed on a bedside detection instrument to meet the clinical bedside detection requirement; according to the invention, the linear transmission mechanism is utilized to realize the up-and-down reciprocating of the adsorption assembly, and the magnetic beads containing red blood cells are adsorbed by combining the magnet blocks of the adsorption assembly, so that the rapid separation of the whole blood sample is realized.
According to the invention, the base can be provided with a plurality of positioning holes according to actual detection requirements, so that not only can the positioning and installation of each reagent strip be realized, but also the synchronous and rapid separation of whole blood samples in a plurality of single-person reagent strips can be realized, the working efficiency is improved, and the lower part of each hole of each reagent strip can be uniformly heated so as to meet the whole blood separation requirement and the subsequent incubation requirement; and manual intervention is reduced, the normalization processing of whole blood separation operation is realized, and manual errors are reduced. The heating unit is arranged at the bottom of the fixed bearing unit, and can heat the whole blood sample in the reagent strip, so that the temperature of the whole blood sample is close to the temperature of a human body, and the separation effect is effectively ensured.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is an isometric view of the present invention.
Fig. 3 is an isometric view of the base of the present invention.
FIG. 4 is a schematic view of a prior art reagent strip.
Detailed Description
The following describes embodiments of the present invention in detail with reference to the drawings, which are implemented on the premise of the technical solution of the present invention, and detailed embodiments and specific operation procedures are provided, but the scope of the present invention is not limited to the following embodiments.
It should be noted that the reagent strip F related to the present invention has a plurality of hole sites, the lower portion of each hole is a tapered structure, reagents matching with the detection items are packaged in the holes of the reagent strip F, and each reagent strip F can realize detection of one sample, and the specific structure is shown in fig. 4.
As shown in FIGS. 1-3, the whole blood separating device in the reagent strip of the present invention comprises
The fixed bearing unit is a base 101, the upper surface of the base 101 is provided with three mounting grooves 102 for clamping reagent strips F (the number of the mounting grooves 102 can be adjusted according to actual requirements), and the wall of each mounting groove 102 is provided with positioning holes corresponding to each hole of each reagent strip one by one, so that the reagent strips can be positioned and clamped in the mounting grooves 102, the stability of the reagent strips is improved, each hole position of each reagent strip can be ensured to be uniformly heated, and the incubation effect is ensured;
a linear transmission mechanism disposed at one end of the base 101; and
an adsorption component is arranged on the linear transmission mechanism, the linear transmission mechanism drives the adsorption component to return up and down, so that the adsorption component adsorbs magnetic beads (which can be used for adsorbing blood cells in a whole blood sample) in the whole blood sample in the reagent strip.
In order to ensure that the whole blood sample is separated at body temperature, a heating unit is disposed at the bottom of the base 101, and the heating unit is specifically a heating plate (preferably a silica gel heating plate) disposed on the bottom surface of the base 101, but may also be other electric heating components. In the whole blood separation process, the heating plate is used for heating the base 101, so that the whole blood sample is at a temperature close to the temperature of a human body in the separation process; of course, the base 101 may also be heated by a heating plate in the subsequent processing steps to meet the requirement of sample incubation.
As shown in fig. 1 and 2, the linear transmission mechanism includes a mounting unit provided at one end of the base 101, a linear power source provided on the mounting unit, and a lifting member driven by the linear power source; the installation unit comprises a horizontal installation part (a first installation plate 201 arranged horizontally) and a vertical installation part (a second installation plate 202 arranged vertically) fixedly connected to one end part of the horizontal installation part, the linear power source is a screw rod stepping motor 203 arranged on the first installation plate 201 (certainly, linear power sources such as an air cylinder and a hydraulic cylinder can be adopted during actual installation), a screw rod 204 of the screw rod stepping motor 203 is arranged vertically, and the lifting piece is a lifting block 205 connected to the screw rod 204 in a threaded manner. During operation, the up-and-down reciprocating of the lifting block 205 is realized by adjusting the forward and reverse rotation of the screw rod stepping motor 203, so as to meet the up-and-down reciprocating requirement of the adsorption assembly.
As shown in fig. 1 and 2, a vertically extending guide (i.e., a guide rail 206) is disposed on the second mounting plate 202, a clamping groove matched with the guide rail 206 is formed in the lifting block 205, and the clamping groove of the lifting block 205 is clamped on the guide rail 206, so that the lifting block 205 reciprocates up and down along the guide rail 206, and the motion trajectory and motion stability of the lifting block 205 and the adsorption assembly are effectively ensured.
As shown in fig. 1 and 2, the adsorption component includes a mounting base fixedly connected to the top of the lifting block 205 through a screw, the mounting base has three mounting heads 301 (i.e., magnet fixing bases) arranged at intervals, the upper portion of each mounting head 301 has a positioning groove, and a magnet block 302 is connected to the positioning groove through a screw. When the whole adsorption component is lifted, the mounting head 301 and the magnet block 302 move upwards to one side of the base 101, so that the magnet block 302 corresponds to the whole blood hole of the reagent strip F, and the magnetic field of the magnet block 302 can adsorb erythrocyte magnetic beads in the whole blood hole, thereby realizing the separation of serum and erythrocyte.
As shown in fig. 1 and 2, an origin sensor 401 is disposed at a lower portion of the second mounting plate 202, a trigger (i.e., a trigger piece 402) is disposed on one side of the lifting block 205, when the trigger piece 402 and the lifting block 205 move downward synchronously, the trigger piece 402 triggers the origin sensor 401, the origin sensor 401 transmits a detected signal to a processing unit (which may be an MCU or a control system connected to a detection instrument), the processing unit processes the received signal and sends a stop instruction to the lead screw stepping motor 203, so that the lifting block 205 drives the magnet block 302 to move downward to an original position, and the magnet block 302 is ensured to be located below the mounting groove 102, so as to separate a next group of whole blood samples.
During installation, as shown in fig. 1-2, the base 101 is fixed on the moving substrate 600 of the detection instrument by screws, and the top of the second installation plate 202 is disposed on the lower surface of the moving substrate 600 by screws, so that the present invention moves synchronously with the moving substrate 600 to meet more clinical detection requirements.
When the reagent strip F works, the reagent strips F are respectively clamped in each mounting groove 102 of the base 101, magnetic beads (reagents on the magnetic beads can be used for adsorbing red blood cells in a whole blood sample) are added into whole blood holes of each reagent strip F by using a pipetting device, and the reagent strips are pumped, beaten and uniformly mixed; then, the screw rod stepping motor 203 is started, the lifting block 205 drives the mounting seat and the magnet block 302 to synchronously move upwards for a preset height, so that the magnet block 302 is close to the wall of the whole blood storage hole at the edge, the magnetic beads are attached to the wall of the hole corresponding to the magnet block 302 under the action of the magnetic field of the magnet block 302, and then the blood serum in the whole blood hole is sucked out by the liquid-moving equipment; finally, the screw stepping motor 203 is rotated in the reverse direction to lower the magnet block 302 to the original position, so that the next whole blood sample can be separated.
In practical operation, the pipetting device can be a pipetting gun, a pipetting pump, a multichannel gun head adapter and a pipetting pump of the detection instrument.
It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in fig. 1), and if the specific posture is changed, the directional indicator is changed accordingly. In the present invention, unless otherwise expressly specified or limited, the terms "connected," "secured," and the like are to be construed broadly, e.g., "secured" may be fixedly connected, releasably connected, or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.
It should be emphasized that the above-described embodiments are merely exemplary embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive various equivalent modifications, substitutions, improvements, etc. within the technical scope of the present invention, and these modifications and improvements should be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A whole blood separator in reagent strip which characterized in that: comprises that
The upper surface of the fixed bearing unit is provided with at least one mounting groove for clamping a reagent strip;
the linear transmission mechanism is arranged at one end part of the fixed bearing unit; and
and the adsorption component is arranged on the upper part of the linear transmission mechanism, and the linear transmission mechanism drives the adsorption component to return up and down, so that the adsorption component adsorbs magnetic beads which adsorb red blood cells in the whole blood sample in the reagent strip.
2. The device for separating whole blood inside a reagent strip according to claim 1, wherein: the heating device further comprises a heating unit, and the heating unit is arranged at the bottom of the fixed bearing unit.
3. The device for separating whole blood inside a reagent strip according to claim 2, wherein: the fixed bearing unit is a horizontally arranged base, and the heating unit is a heating plate or heat preservation cotton arranged at the bottom of the base.
4. A whole blood separation device within a reagent strip according to any one of claims 1 to 3, wherein: and a plurality of positioning holes which are in one-to-one correspondence with the hole positions at the bottom of the reagent strip are formed in the bottom wall of the mounting groove.
5. The device for separating whole blood inside a reagent strip according to claim 1, wherein: the linear transmission mechanism comprises an installation unit arranged at one end of the fixed bearing unit, a lifting piece driven by a linear power source is arranged on the installation unit, and the adsorption component is arranged on the lifting piece.
6. The device for separating whole blood inside a reagent strip according to claim 5, wherein: the installation unit includes horizontal installed part and links firmly the vertical installed part of horizontal installed part a tip, the sharp power supply is for setting up lead screw step motor on the horizontal installed part, the setting of piece goes up and down is in on lead screw step motor's the lead screw.
7. The device for separating whole blood inside a reagent strip according to claim 5 or 6, wherein: the adsorption component comprises a mounting seat fixedly connected to the upper portion of the lifting piece and at least one magnet block arranged on the upper portion of the mounting seat.
8. The device for separating whole blood inside a reagent strip according to claim 7, wherein: the mounting base is provided with at least one mounting head with the same number as the magnet blocks, and the magnet blocks are arranged on the upper part of the mounting head.
9. The device for separating whole blood inside a reagent strip according to claim 6, wherein: the vertical mounting piece is further provided with a guide piece, and the lifting piece moves back and forth up and down along the guide piece.
10. The device for separating whole blood inside a reagent strip according to claim 6, wherein: an origin sensor is arranged on the lower portion of the vertical mounting piece, a trigger piece is arranged on the lifting piece, and the origin sensor is triggered when the trigger piece moves downwards to an original position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110080996.1A CN112665957A (en) | 2021-01-21 | 2021-01-21 | Whole blood separation device in reagent strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110080996.1A CN112665957A (en) | 2021-01-21 | 2021-01-21 | Whole blood separation device in reagent strip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112665957A true CN112665957A (en) | 2021-04-16 |
Family
ID=75414084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110080996.1A Pending CN112665957A (en) | 2021-01-21 | 2021-01-21 | Whole blood separation device in reagent strip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112665957A (en) |
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2021
- 2021-01-21 CN CN202110080996.1A patent/CN112665957A/en active Pending
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Country or region after: China Address after: 450016 No.199, Jingkai 15th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Applicant after: AUTOBIO LABTEC INSTRUMENTS Co.,Ltd. Address before: No. 199, 15th Street, economic and Technological Development Zone, Zhengzhou City, Henan Province Applicant before: AUTOBIO LABTEC INSTRUMENTS Co.,Ltd. Country or region before: China |