CN112662635A - High-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031 and application thereof - Google Patents

Abstract

The invention discloses a high-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031 with the preservation number of CGMCC No.21406 and the gene length of 135812bp, which is discovered to have no virulence gene and lysogenic gene after sequencing the whole gene, and the gene of the bacteriophage contains a plurality of genes of lytic enzyme. The Escherichia coli bacteriophage RDP-EC-20031 provided by the invention has higher temperature tolerance and wider acid-base tolerance range, and is beneficial to industrial production and preparation; the phage was found to have a lysis rate of 98% by lysis rate test and RTD test, and diluted 10⁷Has good cracking effect on pathogenic bacteria. The bacteriophage has the characteristics of excellent performance and safety. The bacteriophage can be applied to the preparation of medicines for preventing and/or treating diseases caused by escherichia coli.

Description

High-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031 and application thereof

Technical Field

The invention relates to the technical field of biology, in particular to a high-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031 and application thereof in pathogenic Escherichia coli infection of poultry animals.

Background

Avian colibacillosis refers to a generic term for acute or chronic infectious diseases caused by pathogenic E.coli, which often leads to parenteral diseases in poultry. The morbidity rate in the chicken flocks is about 11% -69%, and the mortality rate is about 3.8% -72.9%. With the development of intensive breeding industry, poultry colibacillosis becomes an important infectious disease threatening the poultry industry, and has serious threat to the poultry industry, thus causing huge economic loss. At present, antibiotics are mostly used for preventing and treating avian colibacillosis, which causes the rise of drug resistance and dampness of the colibacillosis. The animal-derived escherichia coli drug resistance not only causes great loss to animal husbandry, but also spreads to human beings through means such as food chain, and the like, thereby posing serious threats to human health and food safety.

Bacterial resistance is becoming more severe, but the speed of development of new antibiotics is far behind the speed of increasing bacterial resistance, and new antibiotic alternatives are sought to prevent and treat resistant infections and to further reduce the use of antibiotics. The treatment with the thalli is a good alternative method for the antibiotic treatment.

The phage therapy is to use phage to infect corresponding host bacteria and replicate inside the host bacteria to crack the host bacteria, so as to treat diseases caused by pathogenic bacteria. Bacteriophages are the largest biological community and also the largest known group of viruses. Along with that bacteria is a virus that can kill bacteria, this fact makes them more attractive as a biological agent, and more promising in the prevention and treatment of animal diseases.

The invention aims to solve the problem of bacterial drug resistance and provides a safe and reliable high-temperature-resistant coliphage RDP-EC-20031 for treating colibacillosis.

Disclosure of Invention

The invention aims to provide a high-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031, aiming at solving the difficulties that poultry in a farm are not treated timely due to the acute occurrence of Escherichia coli infection, and the drug taking is ineffective due to the drug resistance of pathogenic bacteria.

In order to achieve the purpose, the invention adopts the following technical scheme:

a high temperature resistant Escherichia coli bacteriophage RDP-EC-20031, which has a polyhedron stereo symmetrical head, a nucleic acid coating, a diameter of about 70nm, a tail with a length of about 90nm, a tail sheath, a neck connecting the head and the tail, and belongs to the family of Anoviridae; the gene has a full length of 135812bp, has no virulence gene and lysogenic gene, and is preserved in China general microbiological culture Collection center (CGMCC) at 12-17.2020 with the preservation addresses as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 21406.

Further, the phage is cultured at 37 deg.C for 1h under pH of 6-9, and the titer is still 10¹¹The pfu/mL is higher than that of the product, so that the product has good acid and alkali resistance; the phage is treated for 30min and 60min at the temperature of 60 ℃, the titer of the phage is only reduced by 1 magnitude order, and the phage has better tolerance to temperature.

Further, the titer reached 10 at pH 2 for bacteriophage RDP-EC-20031⁶pfu/mL, at pH 12, still survived the phage, with a broad range of acid-base tolerance of the phage.

Further, the phage RDP-EC-20031 efficiently infected Escherichia coli, and the optimal multiplicity of infection was 0.1.

The invention also provides application of the Escherichia coli bacteriophage RDP-EC-20031 in preparing medicines for preventing and/or treating diseases caused by Escherichia coli.

The invention also provides application of the coliphage RDP-EC-20031 in preparing feed for preventing and/or treating diseases caused by colibacillosis.

The invention also provides a bactericidal composition for preventing and treating escherichia coli, which comprises an effective amount of escherichia coli bacteriophage RDP-EC-20031.

Preferably, antibiotics for resisting Escherichia coli are also included.

The present invention also provides an additive for preventing and or treating diseases caused by Escherichia coli, the effective component of which comprises the Escherichia coli bacteriophage RDP-EC-20031 as described in claim 1.

The invention also provides a cleaning agent or a disinfectant, which comprises the coliphage RDP-EC-20031.

The invention has the beneficial effects that:

(1) the invention screens and obtains a strain of Escherichia coli BE-20036 from the liver of a sick chicken in a Qingdao, i.e. Memo-broiler farm, and the pathogenic bacteria can generate drug resistance to various antibiotics and simultaneously contain a plurality of virulence genes through drug sensitivity detection and PCR detection; the pathogenic escherichia coli BE-20036 is taken as a host to separate and obtain a bacteriophage RDP-EC-20031, the bacteriophage RDP-EC-20031 has strong cracking effect on the pathogenic escherichia coli BE-20036 in the poultry breeding environment, and a bacteriophage source is provided for the industrial production of the bacteriophage for preventing and treating the pathogenic escherichia coli BE-20036 in the poultry breeding environment;

(2) the Escherichia coli bacteriophage RDP-EC-20031 provided by the invention is cultured for 1h at 37 ℃ under the condition that the pH is 6-9, and the titer is still 10¹¹The pfu/mL is higher than that of the product, so that the product has good acid and alkali resistance; the titer of the bacteriophage RDP-EC-20031 reaches 10 when the pH value is 2⁶pfu/mL, at pH 12, still survived the phage, with a broad range of acid-base tolerance of the phage. The phage is treated at 65 deg.C for 60min, and the titer is still maintained at 10¹⁰pfu/mL, better temperature tolerance.

(3) The Escherichia coli bacteriophage RDP-EC-20031 provided by the invention and the antibiotic have a synergistic effect, the bacteriophage can improve the sensitivity of the medicament, and the medicament can improve the cracking property of the bacteriophage.

(4) The Escherichia coli bacteriophage RDP-EC-20031 provided by the invention has good application prospect in prevention and control of pathogenic Escherichia coli of poultry.

Drawings

The above and other features, properties and advantages of the present invention will become more apparent from the following description of the embodiments with reference to the accompanying drawings:

FIG. 1 is an electron micrograph of bacteriophage RDP-EC-20031 according to the present invention;

FIG. 2 is a schematic diagram of the experiment of the thermal stability of the bacteriophage RDP-EC-20031 of the present invention;

FIG. 3 is a schematic diagram of the acid-base stability experiment of bacteriophage RDP-EC-20031 of the present invention;

FIG. 4 is a schematic diagram of the genetic stability experiment of bacteriophage RDP-EC-20031 of the present invention;

FIG. 5 is a schematic representation of a one-step production curve for bacteriophage RDP-EC-20031 of the present invention;

FIG. 6 is a diagram showing the lysis test of the bacteriophage RDP-EC-20031 of the present invention against the host bacterium BE-20036;

FIG. 7 is a graph showing the experimental results of the synergy between the bacteriophage RDP-EC-20031 of the present invention and antibiotics;

FIG. 8 is a scanning electron micrograph of the bacteriophage RDP-EC-20031 microcapsule of the present invention.

Detailed Description

The invention is further illustrated by the following figures and examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention uses Escherichia coli BE-20036 separated from the liver of sick chicken in the Qingdao, i.e. the inkland and meat chicken farm, and through drug sensitive detection and PCR detection, the pathogenic bacteria can generate drug resistance to various antibiotics and simultaneously contain a plurality of virulence genes; and (2) taking BE-20036 as a host, separating and obtaining a coliphage RDP-EC-20031 from the sewage of the broiler farm, and determining the genotype of the RDP-EC-20031 through a whole genome sequence.

Example 1 isolation and identification of pathogenic Escherichia coli BE-20036

Sampling from a diseased chicken farm, taking diseased chicken livers by aseptic technique, streaking on a selective medium (Macconk agar medium), culturing at 37 ℃ for 18-24h, forming round, flat, smooth and wet red colonies on the medium, selecting typical colonies, streaking and purifying for 3 times, then selecting single colonies, inoculating into 5mL LB broth, culturing at 37 ℃ under 200rpm oscillation for 8h, and obtaining uniform and turbid bacterial suspension. The Escherichia coli is determined by 16sRNA molecular identification, the serotype is determined to be O78 by serum identification, O78 has strong pathogenicity, and the serotype can be transmitted to human by methods such as food chain and the like, so that the Escherichia coli has strong pathogenic infectivity; it was named BE-20036 and stored in a refrigerator at-80 ℃.

Example 2 isolation and characterization of Escherichia coli phage RDP-EC-20031

(1) Sewage treatment: collecting sewage from a farm, centrifuging at 10000rpm for 5min, collecting supernatant, filtering with 0.22 μm filter, and collecting the filtered supernatant.

(2) Preparing a mixed bacterial suspension: 0.2mL of the bacterial suspension and 0.3mL of the filtrate were added to 5mL of LB broth, incubated overnight at 37 ℃ with shaking at 200rpm, and then centrifuged at 10000rpm for 5min, and the supernatant was filtered through a 0.22 μm filter and the filtrate was used.

(3) Phage separation: separating phage by double-plate method, mixing 0.2mL of mixed bacterial suspension filtrate and 0.2mL of Escherichia coli suspension, water bathing at 37 deg.C for 10min, spreading double-plate, culturing at 37 deg.C for 8-12h, and observing result. If there are phage, there will be clear plaques on the plate. Selecting single plaque in 1mL physiological saline, carrying out water bath at 37 ℃ for 30min, centrifuging at 10000rpm for 5min, filtering the supernatant with a 0.22-micron filter, taking 0.1mL filtrate and 0.2mL bacterial suspension, carrying out water bath at 37 ℃ for 10min, paving a double-plate, culturing in a 37-DEG C incubator for 8-12h for purification, purifying for 3 times according to the steps to obtain a phage, and naming the phage as RDP-EC-20031. The plaque diameter formed by RDP-EC-20031 on the double-plate is 1mm-2 mm.

(4) Preservation of phages

Phage multiplication fluid was mixed with 60% glycerol according to 1: 1, and storing in liquid nitrogen.

Example 3 morphological Observation of phages

Dropping 20 μ L of liquid containing crude phage particles on a copper net, naturally precipitating for 15min, sucking off excessive liquid from the side by using filter paper, adding a drop of 2% phosphotungstic acid (PTA) on the copper net to dye the phage for 10min, sucking off the staining solution from the side by using filter paper, and observing the form of the phage by using an electron microscope after a sample is dried. The results are shown in FIG. 1.

As can be seen from FIG. 1, the phage RDP-EC-20031 has a polyhedral, three-dimensionally symmetric head portion, which encloses nucleic acids, has a diameter of about 70nm, has a tail portion of about 90nm in length, and has a tail sheath, and a neck portion connecting the head portion and the tail portion. According to the ninth report of the organization of viral taxonomy of International virology, the phage can be classified into the family Myoviridae of the order Narivulales.

Example 4 phage genome sequencing

After enrichment culture of a single phage, centrifuging for 15 minutes at 8000g under the condition of 4 ℃, adding 10% PEG8000 and 0.5M NaCl, standing overnight, adding equal amount of chloroform, mixing uniformly, standing for layering, centrifuging for 10 minutes at 5000g, removing a chloroform layer and a PEG layer, adding restriction endonuclease for digestion treatment, suspending the phage under the condition of gradient density cesium chloride, dialyzing for 3 times by TM buffer solution at the later stage, and performing whole genome sequencing by Chimona genome biological sequencing company. The whole length of the phage genome is 135812bp, and the phage genome has no virulence genes and lysogenic genes.

Example 5 thermal stability test

Subpackaging the phage stock solution into 50 ml sterilized centrifuge tubes, incubating at 35 deg.C, 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 80 deg.C and 90 deg.C for 30min and 60min, diluting with physiological saline 10 times in gradient, and spreading on double plates to determine its potency. The results are shown in FIG. 2.

As can be seen from FIG. 2, the optimum growth temperature of the phage was about 37 ℃ at which the titer was the highest at 10¹¹pfu/mL or more; the titer of the phage is gradually reduced along with the rise of the temperature and the extension of the time, the phage titer is only reduced by 1 order of magnitude after being treated for 30min and 60min at the temperature of 60 ℃, and the phage has better high temperature resistance; this is of great reference value in the production, transport and storage of bacteriophages.

Example 6 acid base stability experiment

Adjusting pH of normal saline with dilute hydrochloric acid and dilute NaOH solution to obtain buffer solution with pH of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and diluting phage with prepared buffer solution to 1 × 10¹²pfu/mL, the diluted solution was incubated at 37 ℃ for 1 hour in a water bath, diluted 10-fold with physiological saline and then plated on double plates to determine the titer. And (5) counting the results after carrying out inverted culture at 37 ℃ for 4-6 h. As shown in fig. 3.

As can be seen from FIG. 3, after incubation at 37 ℃ for 1 hour at pH 6-9, the titer decreased by only 1 order of magnitude and remained at 10¹¹More than pfu/mL, which shows that RDP-EC-20031 has better acid and alkali resistance. At pH 2 and pH 12, the phage remained viable and the titer of the phage was at 10 at this time⁶pfu/mL or more, indicating that the phage has a wide acid-base tolerance range and a high phage titer, and thus a sufficient number of phages can be guaranteed to pass through the digestive tract.

Example 7 genetic test for stability

Continuously passaging RDP-EC-20031 for 29 times, adding the same amount of RDP-EC-20031 and BE-20036 into each generation of culture medium, culturing for 6h for each generation, spreading double plates, and measuring the titer. As shown in fig. 4.

After the RDP-EC-20031 is continuously transmitted for 29 generations, the titer is stabilized at 10¹¹The pfu/mL is higher than that of the phage, so that the phage has better genetic stability, and keeps higher activity in the continuous passage process, which indicates that the RDP-EC-20031 is suitable for industrial production.

Example 8 determination of optimal multiplicity of infection

The phage multiplication solution and the host were added to the LB broth at a ratio of the multiplicity of infection of 100, 10, 1, 0.1, 0.01, and the total volume of the culture system was ensured to be the same. After shaking culture at 37 ℃ and 200rpm for 8h, centrifuging at 12000r/min at normal temperature for 5min, and spreading the supernatant on a double-plate to determine the titer. The results are shown in Table 1.

TABLE 1 results of the best multiplicity of infection experiments

As can be seen from Table 1, when the multiplicity of infection was 0.1, the growth medium was relatively clear and showed the highest titer after 8 hours of culture, indicating that the optimum multiplicity of infection of RDP-EC-20031 was 0.1.

Example 9 determination of one-step growth curves of bacteriophages

Taking an escherichia coli suspension in a logarithmic growth phase, inoculating escherichia coli BE-20036 and bacteriophage RDP-EC-20031 according to the proportion of the optimal complex number of infection, carrying out water bath for 10min at 37 ℃, then centrifuging for 5min at 12000r/min at normal temperature, removing supernatant, removing free bacteriophage which is not adsorbed on a host, and washing twice by using LB broth culture medium. The pellet was resuspended in LB broth at 37 ℃ and quickly placed on a shaker at 37 ℃ and 200r/min for shaking culture and timing. And (3) sampling at regular time to measure the titer, drawing a growth curve by taking time as an abscissa and a bacteriophage titer logarithm value as an ordinate, obtaining the incubation period and the lysis period of the bacteriophage, and calculating the average lysis amount. Mean lysis is end phage titer/initial host bacteria concentration. The results are shown in FIG. 5.

As can be seen from FIG. 5, the titer did not change significantly within 30min after the phage infected the host bacteria, indicating that the incubation period was about 30min, and the titer of the phage increased significantly within 30-90min after infection, then became stable, indicating that the phage lysis period was about 60 min. Through calculation, the phage lysis amount is about 169 PFU/infected cell, and the phage RDP-EC-20031 has the characteristics of short latency, strong lytic capacity and high replication.

Example 10 determination of phage vs host lysis curves

Adding the phage RDP-EC-20031 into the suspension of the host bacterium BE-20036, and obtaining the lysis curve of the phage to the host due to the change of the absorbance of OD600 caused by the lysis of the host by the phage.

The host bacterial suspension cultured for 6h is inoculated into 100mL LB medium at a ratio of 2:100, and cultured at 37 ℃ for 2h (OD600 is about 0.15, about 5.0 x 10) with shaking at 200rpm⁸cfu/mL) the bacterial suspension was divided into 4 sterilized Erlenmeyer flasks, and 0.2mL (about 3.6 x 10) of phage growth solution was added to 3 of them at the optimum multiplicity of infection¹¹pfu/mL, filtered through a 0.22 μm membrane), while taking the average value with no phage added as a control. The results are shown in FIG. 6.

As can BE seen from FIG. 6, the Escherichia coli phage RDP-EC-20031 is added into the host bacterium BE-20036 bacterial suspension, because the phage has stronger lysis effect on the host, the OD600 absorbance gradually decreases after the phage is inoculated, the host bacterium without lysis starts to reproduce with the time increase, the OD600 absorbance gradually increases until 36 hours later, and the two tend to BE consistent. The bacteriophage can effectively inhibit the growth of host bacteria within 9 hours, and the bacteriophage has very important application value in treating animal diseases.

Example 11 synergistic Effect test with antibiotics

3% of the host BE-20036 was inoculated into LB broth, kanamycin monosulfate was added to a final concentration of 30ppm, and 1X 10 was added¹⁰The change in OD600 absorbance was measured periodically for pfu/mL phage RDP-EC-20031. The results are shown in FIG. 7.

As can be seen from FIG. 7, the OD600 value gradually increases with the time, but the absorbance of the combination of the phage and the antibiotic is smaller than that of the other two groups, which shows that the combination of the antibiotic and the phage can more effectively inhibit the growth of host bacteria, the phage and the antibiotic have synergistic effect, the phage can improve the sensitivity of the drug, the drug can improve the lytic property of the phage, and the phage and the antibiotic have synergistic effect, and therefore, the method has very important use value in the process of treating animal diseases.

Example 12RTD experiment

Dividing the region on the plate, dropping a drop of host bacteria (about 100 μ L) to the center of the region, air drying, dropping a drop of phage with different dilutions to the spot of host bacteria, air drying, and culturing at 37 deg.C for 16-24 h. According to the growth condition of the host bacteria, when the host bacteria cannot grow continuously, the concentration is called CL (the CL concentration is the concentration of the host bacteria in a completely lytic environment and can be used for a dilution scheme for guiding production practice), and as a result, RDP-EC-20031 is found in a dilution 10⁷After doubling, the bacillus subtilis still has better lysis effect on host bacteria.

Example 13 cracking Rate experiments

Under aseptic conditions, 1mL of sample and 1mL of host bacterium solution (1X 10)⁵CFU/mL), incubating for 15 minutes at 37 ℃, mixing uniformly, and diluting to 10 with physiological saline^-1-10^-3Each gradient was applied in an amount of 100. mu.L, spread on LB agar plates, incubated at 37 ℃ for 24 hours, and each gradient was repeated twice. Simultaneously taking 1mL of physiological saline and 1mL of sinkMaster bacterium liquid (1X 10)⁵CFU/mL) as a blank, repeat the above steps. Plates with 30-300 colonies were picked for enumeration. The test was repeated 3 times and the average was taken. The phage lysis rate was (1-number of treated colonies/number of control colonies) × 100%.

The result shows that the cracking rate of RDP-EC-20031 reaches 98%, and the method has a good cracking effect on a host and is suitable for being used in the culture process.

Example 14 this is an industrial process for producing bacteriophages

1. Seed preparation

(1) Preparing a host seed: selecting a single colony in an aseptic environment, inoculating the single colony in 5mL of LB liquid culture medium, culturing at 37 ℃ and 200rpm for 4-6h, inoculating the cultured host bacteria in 5% of the inoculum size in 100mL of LB liquid culture medium, culturing at 37 ℃ and 200rpm for 4-6h, inoculating the host bacteria in 5% of the inoculum size in 1000mL of LB liquid culture medium, culturing at 37 ℃ and 150rpm for 4-6h, and placing the cultured host strain seed in a refrigerator at 4 ℃ for later use.

(2) Preparing phage seeds, namely, after properly diluting the preserved phage seeds, putting 200 mu L and 200 mu L of host bacteria into an upper layer culture medium, pouring a double-layer plate, culturing for 8-12h at 37 ℃, selecting a plate with a cloudy plaque, washing the upper layer culture medium by using 5mL of LB liquid culture medium, then, centrifuging at 12000rpm for 10min, filtering through a 0.22 mu m filter membrane, and collecting clear liquid.

Inoculating the clear liquid and the host into 5mL of LB culture medium respectively in a proportion of 2%, culturing at 37 ℃ and 200rpm for 4-8h (until the proliferation liquid becomes clear or floccules appear), inoculating the cultured phage and the host into 100mL of LB culture medium simultaneously in an inoculation amount of 2%, culturing at 37 ℃ and 200rpm for 4-8h, inoculating the cultured phage and the host into 1000mL of LB culture medium simultaneously in an inoculation amount of 2%, culturing at 37 ℃ and 200rpm for 4-8h, and placing the cultured phage seeds into a refrigerator at 4 ℃ for later use.

2. And (3) phage multiplication:

inoculating the prepared host bacteria into a seed fermentation tank according to the proportion of 3%, culturing for 3-4h, then inoculating the host bacteria into the fermentation tank through a seed transfer pipeline, wherein the inoculation amount is 5%, culturing the host in the seed tank for 3-4h, inoculating the host into the phage according to the inoculation amount of 5% when the OD is 0.7-0.9, culturing for 6-8h, and after the dissolved oxygen is reversely increased, the pH value tends to be stable, and ending the increment.

Note: fermentation tank parameters: the seeding tank is 200 ℃ and 300rpm, the aeration is 1:0.6-0.8vvm at 37 ℃. Fermentation tank: 120-150pm, 37 ℃, 1:0.6-0.8 vvm.

The components of the culture medium: 2.5 percent of yeast extract powder, 0.3 percent of glycerol, 0.8 percent of sodium chloride, 0.2 percent of monopotassium phosphate, 0.2 percent of dipotassium phosphate, 20ppm of calcium chloride and 20ppm of magnesium chloride.

3. Phage post-treatment process

(1) Centrifuging: and (4) centrifuging the proliferated phage by using a tubular centrifuge at 14000rpm at the flow rate of less than or equal to 30L/H, and removing uncleaved hosts, bacterial debris and the like.

(2) And (3) adopting a hollow fiber membrane with the thickness of 400nm for further filtration sterilization.

(3) The concentration was carried out using 100kD wound membranes.

(4) And (3) filtering and sterilizing: the concentrated phage were sterilized by three-stage filtration (0.45 μm polypropylene, 0.2 μm double-layer polypropylene, 0.22 μm polyethersulfone).

4. Low temperature spray drying of bacteriophages

Adding the phage liquid after filtration and sterilization into a carrier and uniformly stirring, wherein the carrier is as follows: 8% of water-soluble starch, 4% of polyvinylpyrrolidone, 1% of lactose, 0.3% of modified chitosan and 0.2% of vitamin C. Then carrying out warm spray drying, wherein the parameters are as follows: the air inlet temperature is 150 ℃, and the air outlet temperature is 75 ℃. As can be seen from the electron micrograph in FIG. 8, the phage coating effect is good, and the microstructure of the microcapsule is a concave cavity and shows a oblate spheroid effect. The powder after spray drying is light yellow and has uniform texture.

The phage can tolerate higher temperature, can be produced by adopting a conventional spray drying tower, improves the production efficiency and reduces the production cost.

It should be noted that the specific embodiments are merely representative examples of the present invention, and it is obvious that the technical solution of the present invention is not limited to the above-mentioned examples, and many variations are possible. Those skilled in the art, having the benefit of this disclosure and the benefit of this written description, will appreciate that other embodiments can be devised which do not depart from the specific details disclosed herein.

Sequence listing

<110> Ray union (Qingdao) bioengineering Co., Ltd

<120> high-temperature-resistant Escherichia coli bacteriophage RDP-EC-20031 and application thereof

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 135812

<212> DNA

<213> 2 Ambystoma laterale x Ambystoma jeffersonianum

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ggacttatag cgtcaggttg gtcagggagt aagataaata atgattgggc aatgcatgaa 180

gctcaggctg tgttgattct ggggtacatt gaggcataca gggcatcaaa aagcgatttt 240

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aagctgctta atttcagtga tataaatgcc tacgacaaac tgtttagaaa gaccacgtta 960

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agggacagtg acgggtatat caattttaga atgaacgcac gtcggccttg cgaaataatc 1080

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acagaaactc ttaccctagg tgattttaaa ctagatccaa cacaacctca tcacacagaa 1620

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agcgacaggc agataggcaa tggcttgttc gatctgtact gcgtcaacac gctgccgcaa 1740

ttttatgttg cagataggga tgcttatctg atctggttta gtgtatggat ggcaagcaag 1800

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gaggaaaggc caaattggcc ctatcctgga atgcaatatc cggtagtcta ctgtatgggt 1980

gcggaaaaca tagatcgtca aagattggca aataccattg gatttatgta cgactcccaa 2040

gtttggtata acaacacatt taaaacagaa ggaccagttg catcccgcta tgtatggcag 2100

aggggtaatg aggggttgac tggttggtgg gagatggtag ataactctaa actccaatca 2160

agagcctttg ttgctgcgtg ccgcacacta tatgagctta agaaacataa agaacctgtt 2220

gacgaaagac tgttcctgtt ctgccagaaa tgggcatggt tcctcaacag attccagtca 2280

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gatccggtag gctatcactc tggcgaaatc cttcgcggtc tgggacttta tgctcaatac 2580

cgtggattgt acctttaaca tagggggctg tttgccccct ttcaggagaa agaatggctg 2640

atattaaagt tgtcagaatt gaatctcttc ctgccactac cgcagtgaca gaggatgatt 2700

acctggttgt tcaacaacca gacctgaccc gtcgtgtaaa aattggcgat gttgtccatg 2760

tggatgggac tgtttctcat gtaatctcct ttaaggaagg tggtaagtta aacggcccaa 2820

tggattttgc ctacttcgaa gaggaagacc tctacctgcg ttggaaaggc gaatttccac 2880

acactgtccc tgcactgtct tcaccgtacg ctgatggtgg gattaccgat gctgcatgga 2940

tggtatatac agacccgtcc ttaagagagg agctggagtc taccattggc gcatctatga 3000

tcatgacagc cgagggtcag tctgtacagg atgtaatgga tgttactgtt cagacagcta 3060

acgatgccaa agcacttgca cagcgggtag attttggtac ggtgcatact gtaggcgatg 3120

tcattcacct tgtcaatttt gttggtcctg cagttattga agagggaaga actactaact 3180

acccttctga agcagctggt gaaaaatttc tgaacggtgt ggtatctcgt cgtgatacta 3240

cgactgttga tggtattttc cgtggtgcta cttctggagc catgtatacc attgcagtaa 3300

ccaacggtgt agcaacaaca aaaagaatag cacttcgtga tgaatttaaa cgcctggagg 3360

caaacaatcc aacaagaaca gttatccgtg ctggagatga tttaaatacg gcagggtatt 3420

tgcagcttga tgcaacaggc cgttggggta tgtggaacca gcaaacagcc tcgtggcaac 3480

cccttgctat agagcaaggc ggtacagggg ccagagatgc cgccggaatc cgtatcaata 3540

tcggtgcttt ctataagcaa cgtgcagccc ttgaggcaaa tttcaatatc aataacttga 3600

ctggtaatca ggacggtata tactaccaac cgatgactgc taatgcaact gaggcaaatg 3660

gttatcctgc aggttctggt gctggtcact tgattgtttg gcagaacaat gctaacggcg 3720

gcacaggctg tcgtcaggaa tactatccat tctctaacgt ggatgtttgg tatttgagaa 3780

cctatcaggc caacacaaac cagtggactg catggcagcc gatggtcaga cctagaaatg 3840

atgacacctt cagatctcat atcggccttg gtaaaaacaa ctcccctgct ttcgggcacc 3900

tttacttagc tcaatactca ggagatgtta aatctgcttc aggtattctc catggggata 3960

aatataacac cgatggtgtt cttgagcatg gttataggat ctactccgag gtaagaaacg 4020

acaataaggc gtggttaaca atccacctgc acaaaggtgc aaaaggatct gaaactcata 4080

gatatttagg tttccgcgaa gacggcgtat tagactgccc taaatacatg caggttgggg 4140

atcttactgg tcagctgaca aactgggggc tcggagaatg gattcgtagt tcaggagcag 4200

aaagagggtt ctggggatcc aagaaagccg ccaagatggt agtctgggat ggaggtatgg 4260

atgaatccgg taatggtact ctggaatggg gtgtttataa caaccggaag gccaagtggg 4320

aacctttacc tcacgcagca ggtggtacag gggctacaac cctggcagat gctcagaatc 4380

tgttcaaagt tcctatagct gcgggtgtca aagatttctt aacactgcca agaactgcag 4440

ggatggaaga tgggaaatac tacccaatca tcgttagaac agatccgtac tatgctccag 4500

caactggcac tgatattacc ctagtcacta gatcgtcatc cggcggtgac cctatgaact 4560

gcgccacctt acagtgtcac tatagaactg gtggttggac agatagagga gactctttct 4620

acggggtagt gaatttctat caaaatgaaa aagcacttct tgggatggtt gctccaacaa 4680

ggggtaaaca agagtatgtt gctttctatg cagaggctcg tgctttccct gtcagcatat 4740

acgcaagtag aaatgttgtc gaagtgttta ccagagagca agattatcaa gtcggctctg 4800

taacaaacaa tcaggacggt gttaagttcg tcgcacctct ccagtcagca gatttaaatc 4860

tagctgttct cggggataac aataccaaca ccagacctat tgttgacttc aaaggtacct 4920

ctgggttcta cactggtggc ggcacacagt ggcactatat aggcactgct gaacgctatg 4980

cggtgatgag caaaatgaac atgccaaaag tagagctatg ggcagacggt attgactatt 5040

tgtgctacgg cagtcctaga aaggcgttat tctctaatgc aggattccag tgtgcatcag 5100

atgggacaga tgatctgact aacggtacgt ttacctctaa atgcggaaat ggtgctggcc 5160

tccaaggcca agcagagttc aggtctactc cagaagctgg tcaagttatt gttcgtgatg 5220

ttgtaggttc cgctcacaga ttctacaatt tcaacaaaga cggcactttc tcagcccccg 5280

gtggttttgt atgtcatact ggtggagact ggaacaacca gtttgggaat aataatccgt 5340

ccaaaataat ggctggtaac gtcaacggac cagaaggttc gatggttgtt ggtgggctat 5400

ctgtggcgtt ttctggaaac tacgctttcc aaatggctgg tcgccttgat cagttataca 5460

ccagatctat cgagggtggt aaccatagag cgtggaacaa ggtcatacaa cacagagggc 5520

aaggtctggg gattaatgac cttaacgact ataaagggga tagggaaggt ttttatcatc 5580

aggaggccaa tgctcaagcc acacctgata gaaactaccc atgctcccga gcaggtacgc 5640

ttatagtgta caggaactac gccaatgttc cagagagttg cgtacaggag tatatatcat 5700

atcttgggga aaaattcctg cgttatggaa caacaatagg cagtgttttc acatggggac 5760

cttggcagca gcttggtggt aagggaatta ctcttaaaac cgcaggctcc aatcttgacc 5820

cggagttcat gatacataca ggtggcggga ctgatgttac caacacttcc aacatgcctt 5880

ctaatacagg cagaatgtac tactggggta atggcgcaga caggaagaac gtgcttgagt 5940

ttcagataaa cgacaacgct acatctgcca gttgggtatg gcattgtggt gctaagacag 6000

atgaggccaa gtcaaggtac cttgctgtca acggcgtagt agagtgcacc gctgtcagcc 6060

agagctctga ccgtgacctg aaggataaca tagaggttat tcctaacgcg ctggaagcta 6120

tccgtaagat gaagggctat acctacactc taaaagagaa tggtatgcct cacgcggggg 6180

taattgctca agaggtcatg gaagcattac ctgaagcggt tggttctttc gtgaaaagga 6240

aagaggttcc tggtcctact caagatggta ttcctctgat gaccgaggaa agattctaca 6300

atgttgacta tgcggctgta acaggattgc ttgttcaggt ttgcagagaa caagatgata 6360

gaataacctc ccttgaggaa caggttaaaa aactaacaga ggttgttacc gagttacaag 6420

gaaaattgaa gtaactgttt tgccctcgct tcggcggggg ctttttgttt gtattgcgta 6480

ttaacttaaa atctggtaaa ataattatca tatttgttta acctggaggc acaatgtttt 6540

tatgggaaag gcctagatac ctcccagata ttagattgga gttactggca gacatcgtta 6600

catttaaaag cccgtttaca ggtaagacgc aaaaagttac ctatgccacg cctaactgga 6660

aaggcacttt gacggtttcc aatatcagcg agtctcagtt gttggagctt aaaagttttg 6720

ttgatcaggt ttacaaagat gggaacattt ttcttatcaa ggcctatggt cattcagtga 6780

cctccaatga gccaatcgct ctcggaccga ctggtaacga cgattttttg aagacggttg 6840

gttgggagcc taacagggat atagccagag ctggggataa aatatctgtc aatgatgagt 6900

tgaaagtggt gacagcctca gtgcgaagtg atggtgttgg ggccgctgtt ataaacatat 6960

ccccaagact taggaaacct attgccgcag gggataccat gaaaataata actcaatctc 7020

cgaagggtag atttcatatc cgagatcttt ataaaattaa gcaaaaggtt gatgctatgc 7080

agaaaggtga caatatagtt atcaacttct ttgaggattt ctaagatgat aagagcgcca 7140

ttagataatt tatttcttga aaagctggag cttgatggtg ttgaaatctt aattgctgca 7200

aagctagagc taccaacagg gactgtaagg gtgtgctcag gaactggtgt tgtggtgatt 7260

gaggggcaac attttatagg gataggtgga tcagcaacat tgactgctgt gtctgagaaa 7320

gatgccacca acttcggatc tgtggtagca acattgaacg ggtttgatcc taaatatgtt 7380

ggcgatctgc tgaagacgga gtattcgggt tgccgtgcca cgttatatat caccgctatg 7440

gagaatggtt tacctgtcac aactaatggc ttgtttgacg gccttataac agacatggct 7500

attcagacag gggagaatag tgctttgtcc atatccatct cttccatctt caacagctgg 7560

ttaagaggtt ttccttaccg atacacagag gaaagccata gttcaagaaa cgataatgac 7620

agatttttta aatatgtaga tcaggtggcg agccgcccga tcatttggga ggtttaatga 7680

gcacacatcc tcgtttagta gactggcaag aaaggttgtg gaaagtattt gacgaagaag 7740

ccaccgtccc gtttgaatgg ggattccacg attgttgcac ttttgctgct aagtgtgttg 7800

atgcccaata cggaacagat tttctttcta actttgaagg aacgtataaa acagagttgg 7860

aaagcaagag atggtgcatg cttcgtttca aaaccacaca cctgcctact gtttttgatc 7920

tctttttgac tcgactggat actcctaaac aggtccagcg tggtgatatt gttattttcg 7980

aaggtctgaa cggcctcacc gctggtgtgt tttggaacaa ccagatttgg gcgaaaggtg 8040

ataaagggat ctttacgttc gatatgaaag attctatcgt tgttgatgtc tggaggtatt 8100

aatgccaatt gcaatgattg ataacatcgt tgcttctgca agatcttcaa gttatgactg 8160

gaacgcatcc aaggcagctc tggctgacag cctgaacact gttaccacca gaactctgga 8220

taagattttc gagggcaaca aatcttacag aagtcagcaa gatagacaaa agcttctcag 8280

atcttctgct gcaccttgct ctgtagtata tggaaagaca cgcacatctg gattgcttgc 8340

gtttttggag caggacaggg acagaaccct tcattgtgct attgttcttg ccaatcaccc 8400

tctagaaggt atagaagata tacttatcga cggtaatcct atttcttcgt atggggattt 8460

ggtatcgtgg gagctacata acgacagaaa aacctctgat cctttcatgg gcacacactg 8520

cccatcgtgg tcacctgaca tgataggtag agggatcagc tggctacggg tcagcttcag 8580

gtttgaccct aacaagtttc ctttcgggtt gccaaacgtc acactcgtca aggttggtaa 8640

aaaatgctat gatcctcgca ccagtaaaga ggtgtatacc aataacgccg ccttggttat 8700

tctggactat ttaagaacgt accttaaatg tcctgacgaa accatcaatt gggagtcctt 8760

taaggaagct gccaacatat gcgacgaagc tgtaagaaac gcagatggaa ccagtgagcg 8820

ccgttacact attaatggtg agttcgacat ggatgaagca ccagcaagta ttatggcaga 8880

aatgctgaaa gcttgtggtg cagatcttag ctatgtagct ggtaaatatg gtctgctggt 8940

aggtgcatac tatggcccag caacaatgac actgagcgag gactgtgttt gtggtgaggt 9000

taagatctat ccggaagcct ccttcgacaa aagatcgaac acaataactg gcagattcac 9060

tagtccaacc aaagggtatt ctgaaacaga cttcccatca gtgttcgtgc cagagtgggt 9120

agagaaggat ggagaaagaa agataatcga tatagattat cgttttgtta ccagtcctta 9180

tcaggctcag cgtgtttccg caatattctt aagacgtgcc agagctggta ggattattga 9240

agtaacctgc aatatgcggg gttttaaatt taagcctggg cgttacgtta cgatggacct 9300

tccaagtatt ggtatagtag gccaagaaat gagggttctt gaatgggagt tcaccaaaaa 9360

gggcggtgta aaggtaaaac ttcgtcaaga tgctaaagag tggaatgatg ccacagggca 9420

acttccagac tctggagatg tagatatccc ggtatctcca tctggtgtag ctcaaccgca 9480

aaacttcaga tactctgttc ttcaagctgg ggaagtaact cacggtgttt tggcttggga 9540

caacgttggg acttacgctc aaaacattgt gcaggtaaga aagaacggag agattatttg 9600

gacagcacaa acagtcgaac agtttgtccg cgtagaagga ttgacaaaag gggcatacac 9660

agcaaccgtg gttgcaacat cctataaagg cggtgtgtct ccagaagctt actgcgagtt 9720

taacattcag gcacctgaag ctccggtttc tgtagaagtt aagcagggat actttgctat 9780

taccttgatt ccacacagta gggacttagc aagtgtaagc acccagtatg acttttggac 9840

atccggtatg acaaggttgc cagacacaag tgatgcaacc gttacttcag aagcaacccg 9900

tatgggtgtt ggttcaactt ggacctctga gggtctgcag aacgataaga tttattattg 9960

gtatattcga accacaaatg cttttggtag ctcccagttt gtagaatgtg ccgcacgctg 10020

ctatacctct attgaagatt tgatgcctca aatagatgct gaatttaaaa agacagagac 10080

ttataaagag ttgatgagca ctttagattc ctctattgaa gaggttgaaa atcgtgtcac 10140

agagcttaac aagtacatgg acggaagggt agatgaggca ttccaacaac ttggagatag 10200

gataggtgcg gtagttacag agacaactca gaagtttgag gatgtcaatg gcaatatcac 10260

agctctggat aggaaacttg ttgctgcaca gaacaagttc accaacgatc tgaacactga 10320

aagtggaaga ctggcatccc taatcgagac aaccaataaa gcaaccactg atcttctgaa 10380

caaaaagaca gaggctttag acgagaaatt ggttgcagcc aaaggggaat tggtagagca 10440

aataggtggt gtggagagcg gataccttgc cggagataaa actctagatg gcaagataaa 10500

cacccagaga acagaacttg acgcaagcat cttgagcacc aaccaggcga cagtggatct 10560

tcttaacagg acctctgaaa ctctggatca aaagatcagt cagacaaacg cgacggtatc 10620

taagaactat gccacccttg atggtaagat aactaccgca aaaactgatc tcaataccct 10680

gattgccaat accaacaagg ccaccacaga tttgttgaat cagaaaacct ccgcactgtc 10740

ggagcagata acttctgcac gtggggagat ttctaccaac aaacaggcta ttgaggattt 10800

ggatgggaag ctgacctcca caaaaacagc actggacgct acaatctccg ataccaacaa 10860

agcaactgtg gatcttatta acggcacagc gtcagctatt cgtcaagaac ttgctgttgc 10920

taagcaggag atcattgatg atgttgggga tgtatcagag cttagagctg cagtggctac 10980

aacatctaaa gctgtgaccg acttggaggg caaagtaaat gctcaatggg gcacaaaaat 11040

acaggtagat tccgcaggga ataagtatgt ggcaggtatc caattgggaa tggaaggctc 11100

tggaggccaa gttcaatcat acttcatggt tagtgcaaac aactttgcgg tatacaaccc 11160

tggaaatgga acagcaaccc ttgctttcgc aatcaagaac aaccaagcgt tcttgaaaga 11220

tgcttttata gagaacggga cgatctcttc tgccaaaatt gcacaagaaa tttcgtctaa 11280

caactacgat ggcaacgggt accacaaata cggttggtat attaacaaga acgggcacgc 11340

ccagtttatg gatgtgtggg tgaaaggtaa catcaacgct agtgcaggta acttcacagg 11400

ggcagttaac gccaccagtg gtaccttccg tggagatgtt tatgccaata atggtagctt 11460

tagaggtacc atagatgcaa caggaggtac attccgtgga cgtgtagaag cttccgttat 11520

ccgtgctaac cagttcgaag gtgcaatagt tgcacatagg acctatggtg actgttctcc 11580

ggtatataac tcgcagcaga gggtttgccg ttggagatgg agatatgtgg acaacgtttc 11640

aggacaaggt aagaacgtaa cattcttctt taaactaaac ggcacacttg ctaattctca 11700

attaaacgcg tggatagcag gtcatcagct gcttgctggt aagaagtacg gaaacgacaa 11760

caacggcatg tgtgcggttg ggataacagg tctaggtgaa caaactatag acattgttat 11820

cgagatctac acaccatggt caacagggaa cgtgacaggg gttacaatat cttgtccaac 11880

cgtaatagtt agtcgttcaa actctagctt ccagggacct tggaacgagt ctcatgacta 11940

acagtaaagc cccgcttcgg cggggtttat ttttatgctc agattgacta ttgttacaaa 12000

atttggtaaa ataccttgta gagattttca taccggagga gtgacgtggc taagtatgga 12060

ttgacagagg ctggtttcgt tataccaact ctaaatgacc taatcgttga aactaagcaa 12120

agcctgatcc gtgcttttgg cgaaaacttc aacgtacaaa gtaactccgt tgcagataag 12180

ctgaccacta tcttcaacga gcgagagtat caacttattc ttatggcagc atctgtatac 12240

tcatcgcaga cgctgtatgg ggcagaaggt atttaccttg atgaacttct tggaaggcaa 12300

ggtatttatc gcagaggccg ttctaaatca tcaggtactt gccagttgac aattaacaca 12360

actgtcccgt acaatatgat ttatgattcc aaaacctaca cgctggatag tggcaacttt 12420

gttcttagca gtgacgtgca ggtggcggga aatcttattg ctcacagaat aaacgctcca 12480

gacctaagaa ttggtaaata caactttcaa attaccaacc agacagatgg aagtatcaaa 12540

acaaaaagtc tggttttaac cgataagtct ctggactctt ctgatttacc aagcttttac 12600

ggcgagatta agcaattcat tgtagataat acaaccttgt tgaacgacga tctaattcag 12660

atagatatgt tgacaggaac gttgtggata ggatacaatt cgaatcttga tcaagttggg 12720

cttaatagca gagtagattt cagagtatct ccgattgtag gtgaaagaac tatcaccctg 12780

gatgttatcg ctaacgaggc aggtgagctg tcaagagagg cagaaactgt aacaacaatg 12840

tcgcctactc caagcggatt tatcaaactt accaaccgag aaagattcaa tgatggtagg 12900

gatgttgaga aggactcaga gtaccgccta agagcctcca gcactaaaca gttgacatct 12960

aaagctaccc gccctgcaat ccttagcgct gtaagcgaag tgaaaggtgt tgagaaagtg 13020

agggtgttct ccaacaacac tgataaaaca gatgcgaaag gtataccacc ttacaagttt 13080

caagtggtag tgtttggtgg tgcaacagag gatatttgtc aagcgctgta tcaaaccata 13140

gcttgcacaa acagaactta tgggaatatc ttctacgata ttaccacatc cgacggtcag 13200

actgaaagga tttattattc taaggcaaac acttaccgcc tggatatgag aattacctac 13260

tcaggtgctg cgctctctac taccgaaaaa gatgccatta cagaggctct tttgcaggtt 13320

gttaatggct tggatgttgc cgacacgttg tataatatcc aattagttgg cgctgcatca 13380

gctgctgtgt caataggcag atttaacaga cttgtaattc aagttaaatc tgtgggagcc 13440

tctgacgaag cttacactac aaacgatatt gttgcaaaca tgaccgaggt gtttgacctc 13500

gacgaaagta atataacttt ccaacaaact atttaaggcg gcgagatatg gcagagatta 13560

agtcggatgt caaccacatc cacactctgc cagattttgt tcaggggggt cttgactacc 13620

tgcctggaga ttttctggct gagaaggtta atttagtcac attcttaacc gtgtacctta 13680

aaaggttgga gaatgttgat aagatgctgg taggtttggc tgaaggaagg ctgcttaaca 13740

atgccagcgg aagatacctt gacgaagttg gtaatcaact cggtattctt cggaatgggc 13800

tttcagatgc ggattttcgt gctaccctgg ttattcagca agcagcagca tcaaggggtg 13860

ggactcgcga agatgtcatc tccaccttgc gacaattgtt cggaagagac aatttcgata 13920

cttggaaagg ggacaacttc aggtttgaca taaacattag aaaaacctgt tttgacatac 13980

tgcagtctat cgaccagatt ttagatatgc tgccattgcc ttgtcatttg cgtcttacag 14040

agtcgcaagg gcaagctttt ggttttgaag gggactctac agctctcggc ttcgggtcgg 14100

tgtgggaaga aaaccagttc ggtgttggcg ggttagctac tttgctatat gttcctgatg 14160

taagacctga ttgggacacc acaactattt attgtgaaag tattacagta aacgcaacta 14220

aacaggaggg ttaatggcaa cagctatttt taaaccttac cgtgattact ctacaatggc 14280

tgcacggttt cattaccgca cagttcaaga tactgccgtt ccaggtaggg attatgatca 14340

tgttgaggga gatgcaacta tcccggttgg ggcaaccagt atcgagatcc ctgtagaaat 14400

tgttgacaaa ttgccaaaca ggttgccaag aagcttcttt atggagtttt catcaaactc 14460

tcaaggggtt atgattggaa cacaaagagc aaaatgtaca atagtttcag atgagaatct 14520

tgacagaata tcttgggata ccgttgagga gagaatgttt cacccaaggt attgggttac 14580

aaccacacag cagactgaaa gcacctgtat tgttgcggat aataactgct gtattaaccg 14640

tttagtgtca agaacatatg gaggcatggc tgggtgcatt tgggaaacag ttgacaagta 14700

tgaccatttc ggcattggat ttgatgatca ctatgagatg agaaacacca agctttggtt 14760

cagaatgtca ataactaatg ccagcaactt ctccacccct gaaaagatga ttatgactgt 14820

agaccttgtt gatggcacaa tcatctatgt ccctttagct caatatgctg tcagcatctc 14880

agaggataag aatgttgcag aaattcatat tgatttcgaa gactgcgtcg ggatggacca 14940

gaacaacaac atgatgggtg ttgatcctcg gcaggtgaga aggattctaa taccgcttat 15000

gcctaaagat tgggtaagta atagcacaga cccgagaacg gaaaatgtag agtgtgagct 15060

gcgtctagat ctgcttcagc cggacactgg ctggaaaatg atgcaactta acaatatcca 15120

ggtgaaagag cacgacgttg gaatctgcac agcatatgac gatatgtgga acgtatctcc 15180

tttaagggtg ttgagcaata tcaagcgtct gggctatacc ggaaccataa accactatgt 15240

tggtatgagc cattattatg attacacatg gtccggaacc caatggtcta taaacagaac 15300

aggggctcta aacgctgcag cttataaatg gcacgatgac tttatgtata atgccaagcg 15360

acatgacttc gacgttatgc agtctgtaag ctttgagctt tatagcgatg cctgcccgtt 15420

ggaatggacc cagagagatt ggaacgataa ctatgctaaa acaggctata cgccttgtag 15480

ttacctccta tctccaacca ttgaagaggg tatgaatttc cttactgcag tatttaagaa 15540

ttttgcatca gccgctatga gaaacaatct gccagtgatt atgcagattg gtgaaccgtg 15600

gtggtggttc aataccgata ctcgtaagcc ttgtatttat gactacccta caaaacaggc 15660

gttttatgat gagactggtg aatatgcact agacattggg actattgacg atcctaaaac 15720

tggcggtgtt tatgacaagt atgtagcatt ttgtcgtggt aagcttggag ctcgtattgc 15780

agctatttca aaggcaataa aagcacacgc tgcctcggca cagatgacag ctttgctgtt 15840

tttcccgacc attatggaaa ccgagcttac gcaaaagctg aaccttgctg accaatataa 15900

aaaggaagca ggtgctcttg atttcttctg cactgagtgc tacgattggg tgatgcaggg 15960

cggtattgaa aaggcaaaag agtctgtaaa catacctatc gttaagttag gttggcaacc 16020

gtctgaaata caataccttg cagggttcgt tcctagcaaa gagcttgctc cggtatatgg 16080

ctacgaccca accaggaatt accaagaatt tttatggaga tgtatctgcg gaaacatggc 16140

aaccatagag tatagatacc ctgaggtaaa acagtatgtg tgggcatacc cgcaagtaat 16200

gtctgacagc atcactgtta ccgccagaga ttctaccgtg cttcatatgg ggcaggttgc 16260

tctgaaaggg tatgtagaag atgttgtccc gccagacttc tcataatcag gagataaaat 16320

gtcaaaacct agttttccat tagagatttg ggcagaggag gatcaggttc tgcctaacac 16380

ccacagacag aacaggctac gcccgattga tgatttgtgg agaaaaggtt gggatcttgg 16440

tcaaaagcca tcctgtgaag aacttaacta tattttcaat atgctcggga cttgggcaaa 16500

atacattgct gacgagcaga tccccgctca agaaggtcgt tatctggtac gtgataataa 16560

cttgagtgat cttttgaaca tccctgttgc ccgtagaaac cttggaatta ttactaagga 16620

agaggcagac gccaggtatg taaaagttac aggcgataca atgacaggcc cattgggttt 16680

gcaacgtatc aactttaaag ctgccgagac agataaggct tggatagaga caactattgg 16740

cccagataag acaactcttg actttgggtt aagtgataat attggggcgt tcgatgatgg 16800

tggcacatct acagtagatg cgttccgttg gagattccag ccaactcagc cggacattaa 16860

cccagagttc actctgatgt acctgaacgc gatcacggct aatcgtgcgt tgttgaaagt 16920

ggtgggcaat gttgaagttg ttgataacat gcgatgtaat aacctcacca ttagcagcac 16980

agcaacattc accaactgca atgtagctag tcagttgacg gctgggtcag tatatgtcaa 17040

tggcggtgca agttgtgaca gtatggttgt aagaagccag cattgtgttg tcggtaacag 17100

aaatgtggtg cgctctgtga atggagtgac ggctaacggc aacggggatg taacgatcac 17160

catcccgcag acaggtgtac aggatatcag aatcggtgca agacttgttg atggtgtttc 17220

tgaatctcca gttcgcaacg gctacgttgt cacaggttgg cattttggtg ataaaaaaga 17280

gatgcgtggt tcaacctact gggcagggcc tttacaaaaa cttgtaaatg gtcaatggat 17340

cactgttaat tatgcgtaga tcggaggact agcatgaaaa tacttcaaga gtttaagctt 17400

taccagccgg gatctggcga atactacacc aacaaggaga ccaaggagct gactcctcag 17460

gaggttgagg attacacggc atacactgga tattttttac gtaatgatga tggcgattgg 17520

tatgtgcaat cacgaacatt acgacaagaa aatccgggct gtatcttcct acttgttgac 17580

gagaagggta ttcttaagac atctacagaa gaaccagatg ccttgtggcc tgctccaggt 17640

cttcgtgtgg ttatcgccaa aaaagaagag gtaccagaaa acattatgct ccaccacgac 17700

gctttgcgtt tagttggtga tgaatttgtc acagacaatg aattctttgt tcaacaggca 17760

gagggtgtca tagagacaga acttgcttgg gctactgcta gaattggtgc ctatcaagac 17820

atgattgacc ttgaatacga tcttactgat gaccaaaaac gcaacatcag agatttgaaa 17880

atgtacaggg taaaacttct tgaaatagat acatctaaag cccctgacat tttcttcccg 17940

gaacgaccta ccctctaaga ggaggtgttg tggagttgga tttggcggca ctgtacggta 18000

tctgcatggg taccgttgtc ccgatctgtg tatgggcttt caaccagtgg cacaccaaat 18060

ggaaaggcat ttccaagagg ctggatgagc tggagaaaga cctcatttta gtgaaaaata 18120

gcatggttac aaaagataag cttgattcca ttatcgagac ccggttaagt aagctagagg 18180

ccgatattaa cgaccttaag caagatgtca ggacagatgt ctccggtatc agagccgatg 18240

tgcaaaaaat tttacacatg cttgtagagc atagtaaaag gtaaacaaag gtcagaacgg 18300

cagggatgcc gttcttgcat tttgttctct aaagtgttaa aatagtcctt aaggaatttt 18360

ctcaggagaa cgtgatgact aaaccagtta tccctcttga tatttgggcc aatcaggatg 18420

ttgtgcttga aaatacagga gagttgaaca aatctcaacc gaacaaagaa gagagattga 18480

aaggttggga tatgacacaa aggatagcat gtgaagttgt taactttgaa ctccacatgc 18540

tctctgcgtg gctaaagcat ttaacagaag atgttgtccc tggatgggat aatagattct 18600

taagggtcac taataatctg gcagacgttc cagacaaagc tgctgcacgt caagttctca 18660

atgtatactc acctgaagaa atggatgacc tttatgttga caaggctgga gacacaatga 18720

tccagactca aactttaggt cttcaaagaa tcgagtttgc atccgcgtca acagacaatg 18780

cggcgatata caccaccata actccagaca agacgatgat ggatttctgg attatggaca 18840

acatcggtgg tgctgacggc acagaccttg atgatccctc tcaccaaata gacggtttcc 18900

gttggagatt tcaaccttcc ggtgggcagc ctgtgttttc ggctatgaag ctcaatgcta 18960

taactgccaa ccgtgcaaga ctggctatcc aaggaaatct tgaatgcaat gatgcaaaaa 19020

gctcctctgc aaacgctaat gcggtaacga tacctagagc cactgttaat ggacaacaat 19080

cctgcggcag cgctcaaata ggtggaagag tagattgtaa cgataaaacc gccaggggtc 19140

aatatgcaac agttggtgga agaaacgtgg tccgcagggt aaacggtagt agcgcagata 19200

gtagtggtaa ccttactatt cctatgccta gcaggggtgt tgaagatgta aggatagcaa 19260

accgtttcag aaccacctac agagaatcaa gagtataccc tgggcatgta atgtgctccg 19320

gcaattacgc ctataaacac ccgtatcagg gcgactattg gactggagcc attcaaaaat 19380

ttgtaaacgg tcaatggatc accatatctt atgcttaaag agggtaaatg aaattaactc 19440

agtttttatg gggactggtt gcagcatctg atgaccctac aaaagtgtcc caatctaagc 19500

tatggtcaaa cataggcatg gcagtgatga ctggggtgtt tattcatatg gggtacaatg 19560

atacgcttcc tgaatggtat gcgtggatat acgcaccttc agtagcctgc ccccagctta 19620

ttagcaaatt catctcgctc cggtggggtt gccagcaccc tgaccacgtt aaaaacgaag 19680

agcaaaaagc ttaaggagaa atcatggcgc aagacatgac aagcttcgag caggcggtag 19740

atcaagtaat tgttgattct gaacgtttgc acttgattgt caacggtaac gctgtggatg 19800

aggttgtcgt agaggatggg accaccatcc ctacggtacg aaaagccatg cttgacaccc 19860

tttattttaa aacgccacca atcccttggg cgtatggtgc atccacaaca gtttttaacc 19920

agttgtatga gtttaaaggg gatacaggcc ctcagtggtg gtacgcccca gcagcatcaa 19980

aatctaaccc ggttaggatg ccagcagatc cttcacaatc tccgaactgg agattatata 20040

ccgattctgc ggtaatggca aaatactacg caaaacttaa cagcccaaga tttgaaggtg 20100

acccgagagt acctacacct ccaatggacg ataagtccga gtctatagca aacacagagt 20160

ttgttgttga ctatgtggat agcatattta aagccatgga ggggatgaaa gttactgtag 20220

ggtctttggt ggtaaaaggt cttacagaac ttgctaacac catagttggt ggcaccctta 20280

ccctacacgg gcctgttaat ggggctgatt ctactgcacg cttcaggaac ctaatcctta 20340

ctgcaaatac ttctacacta acttttgcgt ggagcgatcc taagcatgca gactggagaa 20400

gcacagagct gcaacctcat gaagtggtca cccacagagt tatagctgac actataactt 20460

ctgggaaacc agtggctaat aacaacgatg tgcattttga tggtctaggt aataactttt 20520

ttgactacgt gtacattcgt ggtaactcca tgaaggcagc aaccgaaccg acattgcagg 20580

ttgatgggac caccagggtt aagaaccttg aggtgacagg caccgttaca gggataacct 20640

actctgtaga tggcaccatg atctacccta gctatattga aagcacaggg gatgcactga 20700

tcaatggaga tttagaggtt ggcggatcta tagtcattcg tggcacagca tctgtccaga 20760

acatatcttt aaatactctg agggtgaatg agcgtgccac ttttgaagga gaagggctta 20820

ccgccaacaa aggtacgatt accgagctga cagccaccac tctaactgca acaactgcaa 20880

actctgaaaa ctgcaacgtt acccgcaact tgcaggtaaa tggggatgtt agtttaaacg 20940

cagcaggtac tggtaccacc tctgttcaca accttgaaat atctggcacg gtgactgggt 21000

ggctgccaga cttctctaat gtcaactttg tctgcaacgg tattaactcc agcggtaaga 21060

taaccacctc tcaagaaatt gaggctggta aaactattac tgcccctact ttccacgcag 21120

ggaaggtaga ttttgatcta gaggaggttg acgcatccgg tgggacatgg acacctaacg 21180

ggcaggccag tatgtatgtt gtccacgcga aaggagattt tacaataggc caatggcctg 21240

gaacatcggc tgaagataaa ccttacccgt ttactgcaat catctatgtc attcaagatg 21300

ctgtaggcca caacgtgact ttgcacgata aatatgccat actgtcggct acccctgtca 21360

ttaacaacaa agccaacagc gttaccttgc tacaattgac atattgtggt gttggtgata 21420

ttgtggatgt ggttattgca caacgttaat atatctgacc gctcctgacg gggcggtttt 21480

cttatctgga gaacacaatg cttccaatcc ctttgctaaa acttggtcgt catttcctcg 21540

ataagagggg tggagttaag gaacttgctg ttctgtcata cccaatagac aacagtgacg 21600

gtacaagtac tacagtagcc tatgtaaata gcttagctat acttttgaca atggtcagct 21660

taatatcaca gggtataaca ggttcgggga gtgtaggact ggggatcttg acacgataaa 21720

ctaccccaat gaggctgcct ggaacgttga tcatgtgtgg agagcagacc gtgcgtttgt 21780

gatccgaact tttgataatc agtttttcta catcggctgt actgcagggc ttattgggtc 21840

tgatgctgca ggcggtaatg atgtttgtgt gagggagtgg acacctcttc ctgaacaaat 21900

cgttaaagga ttgcaccttg acacccaccc agagcgcctt gttgaggtga tgggcggtat 21960

taacaacact gtttgggtta ttgcagaggc agaaggcact ggcattttac acctgtacgg 22020

atctggtaac aacacctacg gatctttgca tgtggataaa aaccagcatg caactcctgt 22080

aaagattggt gaaacatcgg aaaatcctga aacaggtcct tggaaaaacc caagaatcaa 22140

ctgtgaggtg catgataact ctgttatttt cggtggtcct agaggtttct ggattgctgg 22200

gtacgacttt ctgagaaaca acagtagaaa cgaccttgtt tggccccctg ctcagattac 22260

ccgtgacgat ttgagaggta ttcctgcaga tgaggagtgg aaagggttca tgtgcggtcc 22320

gaacggggca gtggttgtaa cacaaagaat gcacagacct gatgaccagc aacttgttaa 22380

cgtattctac gggcaagatg tttggggaga tgatacatgg cgtgccttga atatcaccta 22440

cacccatgag gtaataatgg ctcgtgggta cggcacaagt ggcattttct tcaacaatgg 22500

tactaagcag taccgtggat tctctcgcaa cctttgcaac gatattggcg cacaatctgc 22560

aaacaatagc cctaggtcca attttatcca ctatcaagct cttgccacag ccaaatacgt 22620

tgagcaacgc ctcccagtta gctggacaga aagtgtttat ttccaaggca ttcacagaga 22680

aggtttttta ggaaccttta ctgttgttaa tggcaaactt tggtggtcag gcatcccgag 22740

gggaagtttt gcaggctcta ataacctgtt tggaggtaga cttaatagcc aagggttcac 22800

agaggtccct gagaattggt ataaaaatgt cccagtagac agttggggtg ttgatgatat 22860

tttcgacgtt aacggtgtta gcagcgttag caatatttac attggtgata cggtcaagat 22920

gaaattgaag cctcagccag aaggggctac gttcattatt gacaagattg aactggtaaa 22980

tgctgcaggt acggttgtta cagatgcaaa ttaccaattc tctaccaact ggaaccacgg 23040

tggggccaac gaagttattg ttacccagta taacaggaat gttaacagac gtggcctgta 23100

ctctgtaaaa ataacctatc atgacaaaca tggcacaggc agaacccaca caacaaggac 23160

tttgaactgg aacactatcg tcccggcgta tccgtctgat ggtaaatggc atactgtcgg 23220

tagaaacaaa cagttccatg ttaatgacac tgtatacttt ggactgaatg cggctcagcc 23280

tgcagtagaa ggggatacat cgtatatggt cagactccat agaatggatg ctggatcagt 23340

aacttatgat gttacccaag agatttataa tcagcgacga aatacctatg ataacttggt 23400

gcagacacag gccttatggg agttcaaccc aaacggcaga ggtggtaaaa tgttgcaggt 23460

caacgaacaa aacgggacat cgttgactgt tcacgaacac ccaaacccct ggcctgaccc 23520

tgggaaaccg aatccaggtg ctcgtacact aaaaattgtc agccatgacg taggatattt 23580

cggaatacgc tgggaagcca cagtaagata tgccgacggg acgacaaaca atatagggat 23640

cactcttggt gggaccagtg aggataactc gctgaaaatt gcctacaccc ctagaggcat 23700

ttctattgat gctatggatg ttgttcgcaa cggttacggg gatgtgaaca tcaaagttac 23760

tcttggtgaa catcttggtg gagaaaggat catcatgtat gcctttgatc atgatccgcg 23820

caccaatggg acttatgcaa atcaggcatg gagttacctg ataaatgcac ctgagccgaa 23880

tactaaagag ttttattacg gtatgaagcg agatgtgtgc aagaaaacag ggacacatga 23940

ctggattgct atctgcgtta aagatgaacg aaccgcttgg gacgagcctg ttaaccgatg 24000

gttcataggg attcccacca gaagtgataa gtatgtcgca gaatatattg tgtgcatggg 24060

cggcaccaac ctgaacttgt gttggaacga ggatgcgaat aaatactctg attatgacta 24120

tatgagggat atctcttgca atcagtggtt taaccaatca acaggttacc ctccaagaca 24180

ggcgagagta aaccctgcaa tcttcacaga tacgcaggtt ttcttgacaa aacaggccaa 24240

cgaggttcag accttcaaga acaactatga tcctaacaag tggttttata actgttatgg 24300

cgcatatttc tgggggcctg ctgaactacc tgatacaggt tcttgcttcg aggctgcaac 24360

ctacggtagc gactatatta tggggcaggt caagaagtac aagataatcc cagggcctga 24420

gactctaggt aacgctgttg acccatatat aaatttcgca gccgtgcatg cgaatatgaa 24480

tggtgagaga tatccgttcg acgttccagt tgccaacgga tacagacgtg tcgtgatgat 24540

tattaaatgc gatctgatcg ggaagcaagt tcttgcagaa aacggaacct caacacattt 24600

attcgagact gctctgcact accagtttgc agactcacca tatgcagggg ataaaagaat 24660

atccgacaca gactctaagc gctgtaagaa agttttactt ggagccgggt ggtggtggta 24720

cgagtttgat ttgacagata agttcacaga tgataccaaa actctcacag gtatccgtct 24780

ggaccttggt gagaacatgc ataaaagtgt ttgtgatggc acctacggtg atcctacaat 24840

atacttgaag tatgtctctc tggagcatcc agaggatgca gtaatcggtc ctaaactgaa 24900

attgttcggc acatggattg ctaaggacag agtaggtatg ggtaagaaag tgagaggatt 24960

cctgatagat gctgggacag aggacatgct ggttaatgca gtgtggccta ctttagaagg 25020

cactaactac aacaatgcag ctaggtctat aaactggttt aacattcaca gagctatgtg 25080

gaccaccaac tgctacttgt ggagagagct taacgatcag gctttcggct tcagtaacgg 25140

tagaaggatg gcaatcatct gctggacaac gctgcaaaga tgctatgacc atgactacga 25200

gattggcggt agggcatgga aggatattag ggatagaatc atatacaatt ttgcagatga 25260

taatggtggc ggggcatata actttggcac aagccgactt atccatttga acggatcccc 25320

tgcctacaag aaagaaggtt actctgggtc tatgatcgag tggggttatg tgaaagatac 25380

tcgcgtattg atgggtcaac aacttgctgc tgcaatagga cctaacgcag tgcagtctgt 25440

taaaccagcg tggttcgata tcccaatatg gtcatctgga acaccgggaa ctgcggcaat 25500

caaccctacc actggggatc tggaaatctc ttgggaggac ttgaagcagg ttggtggctg 25560

ggataagaca ggctaccagg tgcaatggtg gagatctgac ggatcgttag cggctgatga 25620

gtttgttaag gacaatttct acaccatgtc ctctgcaaaa gcacagcaat tgtttggtca 25680

ggccactccg tcaacgatca ccatgtccat gagctgtaaa gacaacagga ctggagcttt 25740

ggggccaagg gttgctaaag ttttctcagg tattaaatgg aatctccctg ttcaaagtgt 25800

ttcatggaag caaataggtg ataacaagct gctggttacc ccagcttgtc agttcaacgc 25860

aactcttaac gttgatcctg ctgttgcggc aaactcagct aaagcttctg acttctctgt 25920

gtctaacact gctatggcag atgtgaggaa gattgacacg ctgaacgcca gaattacctg 25980

taaaaacact tatggcacat tccagatcat caacaacttc acagatgctg attctaaggt 26040

agtgaggaca gcaagccaga ctctgagttt aggaactctg gcctacgcgg ccctgatcac 26100

tgaacagtcg gcaacactcc aaggaggcgg tgtaggcaag tccattgcaa cacctgtgtg 26160

gaagccaaat gagtgggttg tgtttgattt aactgtggac ttctccagcg ataataactg 26220

gacatgggta aggaattgct tgtcacaact gatgggtggt ccaaactccg ttagtgatag 26280

ccaagactcc actgacccga gtgtgtttca ggtgggtaaa actcacccag aaactggggc 26340

accgttgcct gacaggaagt atgctttggt ttgcatctct tacggaaaag cagatgtaac 26400

cttctcaggg acacacacat ataacggcac ttacaacttc tctaaaaagt acagcctcaa 26460

ggcaggtaac attgtagacg aggttggtgc gttgtataat ccaggaaacg ggatagggat 26520

tgtaggcggt aaactgcaga tgcaggaacc ttctattacc ccttccaatg tgccaggaat 26580

cagaaagact tgggaaagta gtaataccaa cattgcaaca gtggatgcca ccacagggct 26640

ggtaacattt aaagctactg ggaatgtcac tataaagttt gtagttacag atgatgcagg 26700

gcgcaaaacg tcttcaacat cttttactgt caaacagatg gcaccacagt ggagaatgtg 26760

gataggtaca gcaacaaacg gggcatatcc taatccggca gggacttctg gtatgaagac 26820

tttctctaca agcaagccga tggagtacgg cagcggtcct aaggtagggc agatggtgta 26880

ctttggtgcg tatattcctg aaattatagg gcttccgaga agtcagcttc agttgctgtt 26940

tggggctggt gttgacgatc ttgccacttt cgggtataga gacaacatcg atgctgcaag 27000

gagttcagga tgggtagggt tcagaatgga gtctggaaag gaaggcagga ttctagggac 27060

agcctctata ggtgttatgt tccccggtga ccagcagtat cgtctagaag cctacgcaac 27120

cttttctcgt taacaagggg gtaaataaaa aggggccaaa cggccccttt ctttttatag 27180

ttagtaatac tcccaacatg ccccatcttc agacggcttg acgcgcttac ctttatggtc 27240

aacataggta tagccacaac cttcacaaat aacaggtgct gcatagcctt ccttccactg 27300

atcctcagtg atcagacctt ccaaatcttt gcagtcaaag ccaaacaact cttcagagca 27360

atctttacaa aaatcagcca tttctcagtc ctcgcattta acaaattttt ctacagatcc 27420

accaagcaca gcttgttgtt tgatggcact ttcttcatca tagtggacgg acttatgttc 27480

cgtacctccg aacttacctg tgtggaaagt atgcacccaa atatctttag gaagggattt 27540

attctcttcc tccaccaact cccatatacc ccacaagtga tctgtgatat ctctgatgaa 27600

atatccctcg ccgaggtttg tattcacact ataagctgat tcgtgatctc cgcaaatata 27660

acggaaggca gtgtactctt tgcctggtgt gaagtgagta tccattagta tggctgttaa 27720

agaatgaggg tctcgaaagc ccaagaactt tagtttaaca atttcgacat ttacatctgc 27780

aagattatcc atgacgatct cctctgtttg gttggtgaga attatatata gatctggtgt 27840

tggtgtcaag cttaatttta agagatctga gagggtattt tattcagaag ggtaatcgca 27900

cagaggtagg cagtttatga aggcacaggc ctcgtttgag gccttaaaat cgattatgac 27960

actttgacgt agcgctctga caaagctgga aagccaacag cagtacctat gctgccgtat 28020

gctatccacc attgaagttc atcgtgacac cattttccga tcagaaaggt gctagggtag 28080

cgagtcagta ttttggcata agctttaggg tctgcatcct ctgggataat gatgcttgga 28140

gcatctttgt tatgaccaca gcagcagttt agagttttca atcctgccgc ccatagactc 28200

atgataggct tagccatgca cttatcaagg cacacacgtt ttttgccgtc acggagcttt 28260

ttaggcacat caacgataac atttggatgc ttggcacctt tcactttcca attgtaggat 28320

ttacacttgc acatttaatc agtctccatc tagaaagccg tactattacg tttttgaggc 28380

ataccttact taaagtcttt atcacctatt acgattgcac gaatccactc attgtgatgc 28440

ttaatggcgg taaggtgtaa cataatgtag tgattgtcat taccaaacac ggcgcgataa 28500

gctttagcag caccatgata taacttccac atcaagtaat ggtatcttcg tttcatggct 28560

ctttctccgg ttcaggatct gggtaatctt caataataaa gggccatccg ttacgcttag 28620

cgtcagactt atacttaatt tccttcattg caaactctag ctgattcttg gctggaatgc 28680

aagctggaga atcatcaacc cagatgtccg ggtagatatc aaactgagcc agataagcgg 28740

ccttctgaac acctccagta taaaacactc caacatcgag ctctttgcgc caatattcaa 28800

tatccacgtt gtcttgagtg atgaaccgaa aagtcacaag gtagactgtc cacccttcct 28860

tcttcaggtc attaatcaag cgggagaaga tgaaagggct cgcattaaac gttccatcaa 28920

agtcaatggc aatggtatgt gtgccgtggc gagtctcata tgggttgctc gtggtaagat 28980

tgctgtagtt aaaaggcact tcttccccgc agtagctgta gtccatattc acaccttctc 29040

gttgataaat ttacgggcgg tttggtgagt tgtttccgct gtcttcagga taatatcgat 29100

cttgttgtca agatccgcca tttcagcagt aagctccgga atctctccgg catgcatggt 29160

gaaatcatac ataggcatgc caagaatctc attgatgcgg cggttgcatt ctgcagcacg 29220

ctggttcatt ttccacgcta ataccaaagc ctcctgagtg gcctctaatg caaagtctaa 29280

tacgtctgcc tcttctttcg tcatctcagt ctctcttgtt gtattgttga taggtgtata 29340

aatcccttat aactcacctt ttattcaaac tgcaatagca agtccaccac cgcgctaggt 29400

ctgtcaacaa actcaccagg tccacatgcc cacagagggt atccgtccac atacttccgt 29460

atccaacccg aagtgtggag ctcaagaagg gcagtgccgt ccttactgtc aaccactctt 29520

tcccacagtg ttgggcagcg aagtttgagg atcggatccc cacctttata tccgtccatg 29580

tagataccca ttgcaaagtt cagcatcatc tttttgtgca ctgacctctt actgagggtg 29640

ctcatcactt cctccttgtg gatgtttgta agcttctcct tggctacctg cagatcgttg 29700

cagccattgt tcagaagatc ttccagacac tttaccctaa ggtcgtgaat atctttgtcg 29760

tgcaccgttg gtacagggtc gtagcgatat agcactgttg atacaaggtt gctagggtgt 29820

gggtctgaca gcaacccata accccaatac tcttctgtat ctgaaaggac aagacaccca 29880

atatctctgt caagctttcc tgtgttgtaa gccacattga ccgccatgat tatagctcca 29940

ctagcaaagt gctttcaaaa tgcctctcat gcagaccgtt ataaggaacc acttccacaa 30000

caccgtcgcg gatatatgga gtcatcatgg tgacataatc gccgaatggg ttggcattat 30060

cactaactac aaccacatgc tgcccctcct tggcagagtg ccagatagat ctgcacttca 30120

tcatgtgttc gagagcgcca ggtaaaatct tgttacgttc tttacgggtc ttcataacag 30180

gtatagctcc ttatgaaagt gggctttcaa tttctcaccg agaaacttct tgataccttc 30240

ctctaaggtg ttttcgatgt agtccagctt aactgcttcg atggacatat catagatcgc 30300

cagttctaag ctgtcaagtc ccatcatagc atgaaccata agattttcat cttcagatac 30360

attcaggggg cttcctataa catctggtgg cagaatagtt tcccaattat tctgggtggt 30420

agctctcccg ccatccacct tacgtcgcag ctcgaaggtg ttcttagtgt aaaacacttc 30480

ataagaaacg atacgactgt cactctcgta ataaacgaag ttcaggtagg agccgagccg 30540

tagtcggcaa acttctaccc caaaccattg ttttttataa gaattcaggc tgatgctgtc 30600

gcattcacgt ttgctcaaca cggctctctc cttttgttaa tcttcgtaaa tcaagatctc 30660

tgctttaggt tttcgtgctt tcaggtccag ctctatccag tgcaccatag atgtttcaag 30720

aaaaacagcc tgtatgcatc cgtcttcagc gtgtcctact agcataacaa gatcttcacc 30780

ttcgtcatct gcatcacgaa catacagaat ctctccagat tcaaagtctt taacaacctg 30840

gttgctggat aatttacgaa tttttgccat atcagaaacc ctctaaaaag ctcttgtaga 30900

aaacaatctc agtatctaca ccagtcacga tcttgtcgtt cctgatttca tctgtaagat 30960

aggtgtcaaa acgcagaacc tgtgtggtgt tgtcacacat ccactcggct gtaaccatgg 31020

cgaaaatctt agcatcttca ttgtcaggtg tgaatgttac cacctcaccg catttaaact 31080

tttcgatagg ctgcgtgtta gctttcggtt ctgcaatggt tatttttgtg gtcattgttc 31140

ttcctccata gtttcaccaa atacttcgat acgagcgtca agctctgttg ccttcagctc 31200

tccaacaaaa tctttcagtt ctttctcgtc aagatcatat gcccacacac aagatgagca 31260

tgcagatttc acgattagca gtgtagatac acgacctgac tctgtaatca tgctcacaat 31320

agttcctaca ggaaacgtgt tgatagcttt tttaactggg cgttcagctt taattactac 31380

ttctgttgcc ataaataaca tcctctctta tttagcgtac tcgatcaatt ctacagtttt 31440

cccaaagctt gggatataat ttttattata cccttcggtg agaacccata aagtatcgta 31500

ctttttccac ttgttgtcaa cagccttttg ttcaaagtta tgctcaaagt ataggtcggt 31560

caggacgata acatcttttg catttggtat gttagcgtcc agatactcga acacgcaagc 31620

ggctgttgtt ccccatgttg agtggacctc atagttgatc atctcgtcga tgttcccacg 31680

agtgtatact ttaacctctc caacctcggt tgaccagcag aacatcgtaa ccttgaactc 31740

tttatacaga ttgcacaacc cagtgatctc attgaacatt ttctttaaag ttctttgtga 31800

gattgaccca gaaacgtcta aagccaccac aatatctata gtgttttccg gattacgtcc 31860

aggaacaaca acgtgctgtt tcttgtttat agccccaact tttcgcattg tgctggtcaa 31920

agatccagat cggcgaccgg gttttctgta ggttagttca gcctttcgtt tagacaacat 31980

acgttgctta atgatgtcta tataattgat tacaggcttg cctctgtttt tgacaaactc 32040

gcgcacagcc tcagggcaac gacctcctga agcttgcatt gctgcttcca acatgtcacc 32100

agagtcctgc atggcctgat ctttatcaga ttgactgatc actggctgtg gctgattata 32160

ccccatagcc tttccaagat catcactagt gccaccgtta ccgctcatgt ggtcatcggt 32220

ttgtcgttta tctccttgag agttctcgcc tttaccgttc ttacttttac cgctattctg 32280

atctctttgc ttatcaagaa ccgcatagac gttctcagca atccatccat ggaacctcat 32340

gtcacagtag caactggcta agaagccaaa aggcttacct tttacaaacg tagtgctttt 32400

ccccttcggg aagaaaacac aaggtggctt gctaaagtca accacaagac ctgtgttgat 32460

aacatgatca caggcaatat tgaacagctc cggctcaaaa cttttgccac ggaccagatg 32520

atcacagata atatgcccca cctcatgcga tagaagaaat gccacctctt tcacagattt 32580

tggggaatag aaaacctcag ccatctcaaa ctgcttgtca gtcaaagatg ctttaagaat 32640

ggttttgcgc tcatccgcca atcccataat gaactcaggg ctgtaataaa tgcgtttatg 32700

gtctgttgcc atagtggagc accatgtgtg gttttctacg aactgcaacc ctgacaacag 32760

tgtgccataa aacggcagct tttcaagaag atacatgcgt gctgcctgca gcattcgcaa 32820

ggcatcatct ttttgtgcgg taatgatttc tgaacttctg ctatccatta aaactccaga 32880

aggggccaat ggcccctgtc attatttaac gtgcttgaaa ataccagcca actcggtcat 32940

tgtcatgcga gtggtcttca taaggtctgt catagccttc tccataattg gagatactac 33000

catgaagtta cctttggtga aacctttgtt ccagtcggta actaataggg gtttaagtcc 33060

ggccccgaga cgctcaccag ttaaagcaca acattttcga atgtatgtgc tcttaaactg 33120

gctgaatgac agatcaactt tcatacctgc atcgtagaac gtctggcaat agaaagtgta 33180

agctttggca atctcaacat cgtcaggcag tgcaggaacc tcttctgtaa gctcttcttc 33240

gaccccttga ggctcttttt cctcttcgta agcctgttgc gtcatctctt caatctcctc 33300

tactacaggc tcgttcacaa tgtcagaaat gtgcttgttg gctccacgat tagccatgaa 33360

gtttttaaaa tcctgcattg gtgacatatc ttcctcccat tcttcgtcaa agagtgctag 33420

tttatcctct tccgttccgt attgcaagat gaaatcttca aaagtgtaat cacctttcaa 33480

tttctccatg cgctcttcgc tcaggtggag catcatctgc tggacaattt ttcgatcagt 33540

tttgtcttta atcaagactg cggtcttttt atctaccaga cagtccttta gagagttaca 33600

cctcaatcct accacacaac agttatcaat tgcgtaagga ccttcatcat ctatccgctc 33660

cagagatggg tagaaaggat tccccagccc gttttcatct gtgtgcatgt cgaaaggtat 33720

aagggtatag tcgcataccc cctctcctaa aagtttttta ccaaatagga ggtattcttc 33780

ccatgacagg ttgaactcta tatctcgttt ccgtgcatta ttttgtttgc gtctgtattt 33840

tttgagtagg cgctctaact cttctggtgt ataatatttt tcaagattca taagttgtct 33900

cttgtgccct gtaggtgaag aaatcttatc aagcgcctca acagatgtca acctcaaaaa 33960

cgggttgact ttactctaaa tccacgatat agtgcaccca ttcgaaagaa aacgaacaca 34020

ggagtactta atcatggcaa atcttaacag ctttagcact cggtggaaac aagatatcac 34080

aaccaatctc tcagcaggcg atatcctttc tgcaatttac gaggctttgg atgataaagc 34140

tacttcaaaa tcggtttgta ccaacccgtt aattcctatg gtaatgaagg tgattgagcg 34200

taggttggta tccggatctg ataagtggac atatctggat aatggaggcc acacttactc 34260

caacggatat gtcgatataa aagtagaata tgcggtatca tatcgccgga gcggctctgt 34320

aactttcaac ttctctggtg gtttccgatc tctggcaact tcaccctttg tttcctcaga 34380

ggagaagttt gaacttccgt tcctttggat gatggctaac gcgacaggca aaacccctgt 34440

aagcattcgc gtaaaataca actacaacgg atctagtaag atctatgccg agagtgtctc 34500

agcaagtttt tattttgtga tgggggatgg aatcctcaaa catataaaga tgtgcgatat 34560

gcatgatgtt ataaatgaag tgactgataa gaatgttctg gtgaaggatc tcaacaattt 34620

ttatacaccg ggagactatt acgtcaaccg tccactgtgc atctttaatg ctgcacaccc 34680

tttaggtgag tatttgatcg ccaatagtga tattgccaat aaccgcttcg aggtagcgag 34740

agtacagaat gaggtattga catcttgcaa gagtggtgat aagtttaccg aagaagagct 34800

gggtgtttta gaaatgatgg gatataagtg cagtctttgg gtagatgagg tgtttaatga 34860

cggtatctac ggacaggata tttacccgtt aaagaagtat actgattcta gtttaattta 34920

cgcggagatt tgactatgat gaagtcatgt ggtagtgtat attatgttgg tgaggatgtt 34980

caggcagata gtgtgtggaa atccattaag tccaccaccg acgatgcctt cttgagcggg 35040

ttatgtttcc ccaatccagg agacattctc cctcaccctg gagattttgt tgtcaacaag 35100

acggacggtt ataccgtgtg cctgatggtc gaaaaaatct ttttagacac tagacacaat 35160

caccgtcttg tgttaaaatt tcaagaatgt aaaatgacag ttgcctaagg aggcctatca 35220

atgtctaaag ttacttatcg tgcagctatt tactgcaatg agcacccaat caataatgat 35280

ggtcactgct tttttaaaga aaatcagttg ggctgtattg ccctccttga tgaaggggaa 35340

ggttatatga ttgcattgac agggtttgag cctgaagcct tggaagccaa tctggactac 35400

ctggtagaga agggtgctgc agacttggtt gccaatggcc tctccataag ccttgaagaa 35460

gtggagcgct tcatgggcaa ttaccgtgta gtgcgtgagt ttactatcga ggtggaaact 35520

ccagagtggg gtagcactga aactaatgct gaagaaaaag aagaggaggt agaataatga 35580

aagtgcaaat cttaaccaat gccgagaaga tgtttatcga agatacgcct tttgaaactg 35640

gtgagctaca gtttgtatat tttgttgact tagataaggt ttttcctgct ctgaatctgg 35700

gtttggagaa agtaccttat aaaactctat gccaagttgt ccgtgcctta gcagcagatg 35760

gggagttcca cacccttgaa gatatccaga cctatgggta tcttgaactg caacgagaag 35820

aagaggtgga ctgataatgg cccagtggaa gacaacagtt gaccttcatg atcagtggtt 35880

aggacgcatc accgaagacg ggaaactagt ggagtgggac aacaagcatg ttagcgagtt 35940

ggcaagtatt gtggctggca aattacgctc caagttccct gaacaactgg atcctgatag 36000

ttcaaagttc gatcctgagt tagaggatgt tgtatactac ttcgataccg ttgatgacta 36060

cgacgcgtgg tttgcagcac gtgagcttta cccacaaggc tctgctatgc gccagatgga 36120

agaagattat cctccgtgtg agcaattcaa tagtgcaatg acttgcctct atgattgggc 36180

agataaaaac cttgtctgga ttcggactgc attttaggtg ggtagggatg gcggcaagga 36240

tgctgcttaa ggagcagtaa atatgttata ccagcagtat gaacttcgtg cagaaaaaga 36300

attaaatttt gacaaagaca ttcacagata ttattatggg aaagttagag ggcaagcaag 36360

aaagtgtact ccaacactct tttatgccag cagacgacta gcgccagaag aggcaacagc 36420

agaaagggtt gcatatggct ggagagttgc gtatgatatt gacagatccg atgtgatgtc 36480

tgttttggaa aactttgaaa acacttacac acaagtagag gacttttact aatgtcaaga 36540

aacgtattcg aaagtggtca aatcactgtc cgcgagttgc cagctattat tcaatttgcc 36600

cagcggcata accgatctgt cctgatttta ggaccgatgg gtgtgggaaa gagtcaggtt 36660

atgaagcaga ttgctgataa tatgttcggt gagcgagatg acaacctggt tgacgtgcgg 36720

ctgtcagata aagatcctgc tgacttatct ggactgccta tccctgtaga ggcagatgga 36780

actacacgca cagtgtttgc aattccagaa ttttggcctg cagactctga ctggagcgga 36840

attatctttc tggatgagct tactcatgca gataactacc tgcagaaggt tgcaatgcaa 36900

attatgctcg accacaagtg cggcacctat gcatttccta agggctctgt ttttgtagct 36960

gcaggtaacc gtgctacaga tggtgctgtt ctttcagcgc tcgaagctcc tctggctaac 37020

cgctttatca ttgtagaagt tacaagcaat gcagaggtat ttattgagga ctacgcattc 37080

cttaatggtg tgcattcctc tgtaattgga tatctgaaaa gagttccatc agctatcgat 37140

aattatgagg ctatggctga gattaattgt ccgtcttttg ccacaccgcg atcttgggtt 37200

gctgcatctg acatcctgca cgattttgac agcggtatta tttctgacgc tatggctcgt 37260

gttcttctgc aaggtgctat tggtcacact gcagcggtag agatctggac ctaccacacc 37320

aagaaacgaa atttgccaga aatcggtcgg attatggcag gtggttgcaa agaatatgta 37380

ggtcccaagc agccagatat cttgtggatt cttgggtctg aaggttgtat tgctattcgt 37440

aatatgatgg aaaatgcaag tgtctctgac gaagatcttg ttagctgtgt ggcaaacttc 37500

ctcgatttcc tttgggaaaa tttcaaagat gaaaaccgag actttgtatt ctctgtcttt 37560

atggcgatga tgaaacctaa cgccttagga aacgccttac ttattggtaa aggccgggac 37620

aagatcgttg ctcagctgat caaagcatac ccgaacctga tgaagattgt aaaagagttt 37680

ggtgaagagt ttagcgccat tttagctaaa gcttgaggag gttgtatggc taaagtaggt 37740

aagcggggaa actacttggt ggtattgacc gaggagccgg agaaggagta cgaatgcagc 37800

tccacttgcg tgatcgatat ccgagaaaaa gatgtagatg aaatctggtt taaaatattg 37860

gacgcttgcg acagcagtga ctatttcttc atctatgtca acgataaagc ggggttcttc 37920

catgacgatg gagagttacc gtatgaaatc tatgaacttg tagaggcttg ccatcgtgga 37980

gatgcggagt tgtttggtct acggctgaaa agcggtaagt tcttctacca agacgaatgc 38040

atgaatcttc acaacttcgt agagggaact tgcaacgatg gagagattct ggcacactac 38100

gactaatttc taagaccacc ccccgcttcg gcggggattt tctatttgga gttattgtga 38160

aaattagaat ctttgctatt attttgagcg cacttttatc gctgcctgtt gttgcagcac 38220

caaaacaaaa agtatatcac acctgcaagc catcggatac taaggagaac attctggcat 38280

gcaatatcta cagggaggcc agaggagatg gcacgatggg tatgctggca tctgggtttg 38340

ccacactaaa caggaaatct caccctaaat accccgacac tataagaaag attgtctacc 38400

aacctaacca gttttcgtgg acaaactacg gcaaaacttt taaggtgaca gaaaaagatt 38460

catgggatca cgctaaaagt cttgcaaaga tgcttctaaa ggtctataat aacaaccatg 38520

ttgcatacat ggctatggat ataacaggtg gagcaacaca ctatcataca acaaaagtac 38580

gaccgaaatg ggcaaaaaag atgcagagga cagctcaatt cgggagtcat atctactaca 38640

aggagcgcaa taaatgattg taaacagtaa tgaaactaaa gcagtgctaa ctgggaatat 38700

ggaggtcaca aggggatcaa tttctctgaa taaagagacc tttgggttaa tcattaaagg 38760

gatttatgaa gataaggtgc tggcaagctg ccgtgaacca atcttcaatg ctgtagactc 38820

ccatgttgag tctggacggg gtgatgtacc gatcattatt cactctccta cagatcttga 38880

accctacttc tttgtgcagg acgttggcct aggcatgtca gaagagatgg tccgcaacac 38940

cttcatgaac ctcggtgagt caaccaagcg caatagcaac gctctggttg gtaataaggg 39000

tgttggttca aaggccccgt tcagcatggt agacactttc gatgtaatca gtgtgcacaa 39060

tggtgtggaa agcacatatc tattgttctt ggatcagggc atcccaaatg tcaccaagat 39120

ccgtgagcgg aagactgaag agcacaacgg ggtaaaagtt cagtttcatg ttaagaaaga 39180

gcatgttctg aaatatcgca ctgctattgc atcatgtctg cgctatgcaa aattcccgtt 39240

caccgtcacc gatccaatga cgagcaaaat gctggagggg gataaggtag aggcgcagta 39300

ttattatgaa aaagatggtt ggaagatgac cattctgaag ggctatacca gctctgacga 39360

gagtcgtgtg gtaatgggtc accaacctta ccgttctaag ttcctggaaa cactaacaga 39420

ttatcctgca atttgtgtag agatccctat cggagactgc aatattaacc cagggcgtga 39480

atggaccata gaaggtcacg acgatggtgg attccaagag cgtttggaag cttttgtcaa 39540

agaaggcatt cgccttcgtg gagcagaagt tgttgcagaa ctggagacag caaaaacatc 39600

ctcagaagca cgcgagatga tgaagaacag tggagtgttc gggcacattt tcggtaagaa 39660

ttttatgtat aaccgttggg aagaaacagg gttaggtagt ttgggggact gcgagatctt 39720

cgcgggtggc ttaaataaag gccgcatagg ctacgcttgt tacggaaagg tagaattctt 39780

acgaggagtc tatttagtat ttaacgacgg aaacggtaaa tataaccgaa ataagtgtaa 39840

ctacttaagc gagatcaccg gacgcaacgt tttctattgc cggaacacgg atctggtaaa 39900

agagtttaaa gaggcggcac accatccgtt ctttgcagat acggtcaaat tgttatcaga 39960

gctccctcgc cgacctgtga agaaaggtga aacaaaaact cgttatgagc ccggttattg 40020

ggttaaaaaa cttgagaagg atggttactt ccgtcgtgag cgtattacca aggcagagtt 40080

caggaagatt aagcactgca taccgtatga gggggatatc caacgtggaa gttgttggct 40140

aggtcagatg agtaacatct accagcaaaa ttatgaccgt gtgcgtgcgg cactccagat 40200

ccctgaaggg gaagatctgt acctggtatc agaatctcgt ctgctttgga ctgatcctga 40260

ttgccagtat gttacagaga agaatgtaga acatttgttg aaagaggatg cttggaaatt 40320

cttactgact cgcgcagcaa atgaaacttc atatgacagc ctgtatagac agttagggaa 40380

aattatccct atgcaagatt ttggaaacta taagccgcat ttccacaagt gggaaggtaa 40440

caccacgtgg aagtacggtg agctgaatca tcgtgccaaa atcttaatcg ccaaccgggt 40500

taagtgcggg aaggcctata ttgcgagatt ggaaaagaaa tatccgcttt tgaagcatgt 40560

aggcttgaaa tattgggaca ctcctgctat gattgaatac cgtgaactga ttgatacaaa 40620

gaataacatc aaataaggag accaagatga ttttcatcta cgaacatttc aaaacccgta 40680

aagaagcacg agaccgccgt gcagaacttg agaagaaagg tttcacatgc gctattgaga 40740

aaggtgacac aacctggaca ctggcagtgg gtgccccaga ggtgttcgat cctgaccgtg 40800

acagcgtctg ctacacccgt gaggattttg aaacccgtga agaggcccgt ttatttcgat 40860

atgggctgga agataacggt aagtacgcca tgatcttaga cttaggcgaa ggttccgtta 40920

aacgttgggg cgtggtatac ccactgtgtg ttgttcacgg tgtggggttc actggggtag 40980

cagttaagtc agaagaacct tcggtagaag tcaaagaccc ggtgctggag gtggtgagca 41040

gtgtgtctca gcaacagact cgaagcatcg agcctttggt agttatttgc ggagaactcc 41100

tccagatcac ctgtaacgaa ggtatttttg aaattcgccg tagcgaaagt gaggaagttt 41160

accagaaagt cttcgaccat atctctgttc aggaatatga cgaggcaatt ggtgaaatcc 41220

ttatctggct ggaacgcaag aatgagttca ctacactggc agacaacctg atcatgaaag 41280

atggtaagct ctactactac ggtgtagaga tgcggtctac cattgcaaag aaaattgaga 41340

aagattatgc agatggaaca ctggatgacc gttatgtgaa attcctagtt cgcctgttac 41400

gcaatccgtc tgcaaaatct gtaaacatgc tctacgattt tatgcaggca aacgacattc 41460

agattgcaga agatggtcga atcatttgct acaaaggggt tcagtttaac ggcgacaagt 41520

gggtggattg gcacagtggt aaagtcccac aataccaggg tgcttttgta tccatgcctc 41580

gtaactttgt agaggatgat ccggaagctg catgctctta tggcctgcac tgtgccagca 41640

aagagtatgc aaagagctac ggcactgtga tgacggttat ggttgaccct gctgatgtag 41700

tcagtgtacc ttaccaacac aacagcgcta agtgccgtgc atgccgctac gaaatcgtag 41760

tagctccaga atcgcgcaaa gatggagacc caatcgagta cgtggttgac cgcgacggca 41820

acactatcga cattatctac gaggaggcgt aatggaaaaa gggcagtacg atgtaggcac 41880

agatgctgcc cttgttgcaa tcatgagtgg tgagaatgtc ttcgtaagtg gaccgggtgg 41940

aaccgggaag acatatctta tcaacatgat ccagtctatg tatggagact cttgcataac 42000

ggttgccccg acaggggtag ccgctttaaa cgttaatgga gcaacagcgc accgaacttt 42060

cgatcttgcc gcaggtgtga gcatggagtc agactggaca gcaataaggg caaaaacagc 42120

aaagcctctg aaaagcaagg catttaccat cctaattatc gatgaaatct ctatgatccg 42180

tgctgacaag ttcatagaga tggatagaaa acttcgcttt cttcggaaaa atgacaaacc 42240

tttcggaggc attcaggtgc tattgtttgg agatttctac caagcacctc cagttgtctc 42300

ttccatggag aaagaagcct acttcaactt ctaccacaca gatctgtgtt gctatacaga 42360

atcttgggag gatcttaatc ttcacaatat agccttagta gaccagttcc gtcaggaatc 42420

tgttcgcttc gccacaatgt taaactgtgt tcgtgaaggt cgtcgcataa aagaggttgt 42480

agcagagctt aacactcgtt gctatcatgg cggtgtccca acagatgcct tgactatctg 42540

cgccaccaat aagcaagcag aggaggtgaa cagacgtttc tatgatgcca tccaggcccc 42600

agagaagacc tacattggta aaatgaaggg taagttccct tccacactgc ctgtggagca 42660

ggaaatgagg ctgaagatag ggatgaaggt tatgataacc tcaaacgatg ttgaccctac 42720

tcacaaagtt ccgtattatg tcaacgggac acgtgccaca gtggtaaaat tcaagaccaa 42780

atccgtggta gttgagctag aagacgggac acaggtcgaa attgagccga gattgtggga 42840

gaataacgag tacaaacctt cccagaggta caatattgcc gaaaggaaga tggagaagtt 42900

catagagagg gttgtgatcg ggtcttatga gcaacttcct ttgaaatcgg ggtacgcagt 42960

aacatcgcat aaaagtcaag gacttacgct agattgttac aacctggacc tcggaaagaa 43020

cggagctttc tctcctggaa tgacgtatgt tgccttgagt cgggttaaga cgatacaagg 43080

aataaacttg ttgcgtccat taagagaggt ggatattatt gttgacccgc gagtcgtaga 43140

gttttataat acaactttcc ctggcttaga cgagaaagtc aggaaagatt ttgaaaccag 43200

agcagaaaag ggggacgtat gagtttgtgg agaaatatag ttaactacgt gtgtggcaat 43260

ccaaatcctg aagagaagga tgttgtgatt cacgatttta ctaaaagatc tcgtgaaaaa 43320

gacctcctgc taacaaaggt gatcgataaa gaccctatag gggactgggc agggtttgcg 43380

ttcacatctg acaagtacac ctttaacaaa ggtgactacc ttgttgtgga ttttaacgaa 43440

gacattgagg gtgctgaacc ttactatcag acccttgtcg ttgcagagtg tgttgagctc 43500

tctgcaaaca tgcacagttt gattcttttt gagcttgact ctgaagacct tcagtagtta 43560

gtaagacgtg agtataaagg ggaggaactc ctccccacaa aactggggag agcaccaaac 43620

gatgagcaaa accaaattag agcttacctg gacaggcaaa aacgagcgaa aaaagttaga 43680

tccgtgcatt ttgttagaag acacgagcaa gtcttatcac gccagtcaaa aggtgaccga 43740

aaacgatatt tttgataata aactgatttt tggcgataac ttactggcat tgaaggcact 43800

agagcaagag tatgcaggca aagtgaagtg tgtttacata gacccgccat tcaataccgg 43860

aagggatttt gagcactacg atgatagcct ggaacactcc atttggcttg gcttaatgcg 43920

ggacaggtta gaaataattc gtaatctgct aagtgatgat ggagtaatat tcgttcactt 43980

agatgactgc gaaatggctt atttaaaagt tttgatggac gagatttttg gtcgtgcaaa 44040

tcagttaaat accatatcga tgaccacaaa ctgtccttcg ggctttaagg caacaggaac 44100

tacagttttc tccacagcaa actttttgct tgtatatgca aaagatcgta ccaagaagcc 44160

gcttaacaaa atctatatac ctaaaggata tgatacggga tataacaaat atctattaaa 44220

cccggatgac cattataaaa gttggacgtg gtgcggtatt gcagatgcgt ttgcaaaggc 44280

caatggctat aaagatacaa aagaggccaa gaaaaaactg cctgatacat ttgatgatga 44340

attagcacaa tttgcaattg ataatgcaga acgtgtattt caatccgttg caattggcgg 44400

tggtgctaag ataaagagaa aagaaactat cgagaagtca aagaaagaca gggataaggt 44460

ttatgtacat ccaaatgagg atgtcgaagg cttttatatc gtaaatggta ggcaaatggt 44520

attctactct aataggcttt tggaaattga cggtgagatg cagcctgctg aactaataac 44580

tgatgtttgg actgacatta gttggaatgg aatatcaaat gaaggaggcg ttagtttcaa 44640

aaatgggaaa aaacccgaag cgcttttaaa gcggattttc gaaatgtgca cgcgagaagg 44700

ggatttagtc ttggattcgt tcggcggttc aggaactacc gctgctgtag ctcacaagat 44760

gaaaagacgt tggatctcgg tcgagctagg tgatcattgt catacccata tcatcccgcg 44820

cttgcaaaaa gtgatcgatg gcgaagatca aagtggtatt tctaaatctg taaactggca 44880

aggtggtggt ggctttcgct attataaact tgcccagaac ttggttgtga aagataagtt 44940

tgactcagaa gaagatttag agtaatattt aaaacattga aggggaggtc tactctccac 45000

acaagataag gagaacaccg aatgatgaag cgttataaac catacacttt tgaaatcgat 45060

ttcgaacttg acgatatgct cactgtagag gctcaggcag ttgtccagct gccagatcca 45120

gagtgccgtg attctgatgt tgattattat ggatttagac agattactga tgtgcatgtt 45180

taccacaacg gagaagaggt agattttcac caactcccta acgatctgcg ccactcgatt 45240

tacaaacgtg cagcacgtga actagattcc tatctggatg cagcaacaac agcagcagca 45300

tttgctgaag aagtaggagg cttctaatga ttacagatgt gaagattatc caataccact 45360

taaccaaatt agaagatgct gaaaaagagc ttgcagaagt tctgaatagc gggtatactt 45420

tactggcatg ctcctgttca gacacataca ttgtgtggac acttgctaag aaggaagaac 45480

cagcaggaat cttggtgcca aaccctaaga ttattagtga ggtggacgac tattcccaag 45540

acaacggctt gccagaaggc tgggaagtat gggaaggtgg tgaaaaccct gcaccaggca 45600

agtgggtaaa atacaaacta cgtagcggtg cacagtatgc tgactactct gatgacctag 45660

actggagtca ctcttcacca ggatcatctg caagtgcata tgatattgtt gcttttcagg 45720

aataaggaga gagtgttatg tcagatttca tgtggaaggt aggctatgcc tctcctgaaa 45780

tgtgtgaggc ttgttttgtg aaaataggcc gcgaggatat tgacaaagtg atccttaagt 45840

ttcgtgatgt gtctgtaatc ggcaaccgag tgcttaagca cctaaagaat ctgaagctta 45900

ccaaaaagaa gttcttcgga cttttcgaag agaaagttga tgcttggacc cattgcatcc 45960

aggaagctaa atctcactac agcgctatcg ttactccaga acgggtagct aagatggaag 46020

gattcatcaa cagcactgaa tttgagtgcc taaaggctgc ccttgatagt tatccaaagt 46080

gcctcaaaca ccttgacaag tatgtggaaa aggatgggga aatgtatctc accctggacg 46140

catactatga gatgcaagaa attttaaatt tagatttgac attggtatcc accgagtata 46200

caataggtat ccgggtttaa cactaggccc tgcccgaaag ggtggggctt tttgcattag 46260

gagacagcaa attgaaattt atctacaaag aacaccacca caaaggtgtt gtgtggagtc 46320

tatttgacgg gtctggtggt gcagtcattg actgggcaaa agctgggtat ctgtgcctgt 46380

gctttaacgc agaaggtgct gaccatggca gttatgcaga ggtgataacc gatcacccca 46440

atattcacta tgtggattat tggattgatc cgtggttccc tcaggaaact ttttgcattt 46500

atcctgctcc agactttatt cttgcgtttc ctccgtgcac gcatttggcg gtatccggtg 46560

ctgcacattt taagaaaaag ctggctaagg accctctgat ccaaattaat gctgtaaaag 46620

atgctcgggt tgcggaagag ttgggagaga tttatgattg cccgtggcat gtagagaacc 46680

ctgtaggggt tatgtccaca ctgtggcgta aacctgatta tcgttttcat ccttcggatt 46740

atggtggtta tttaccagaa gatgatgtaa acccatggtt tcctgaattt atagccccac 46800

gtgatgccta tccaaaaata acttgcggct ggataggtaa tggatttata atccccgcca 46860

caaaacctgt cccgcaaata gtggatgcgg ttagtggtta ttctatgcaa cacgctaagc 46920

taggtggaaa aagtgcaagg acgaagatga ttcgaagcct tacaccacgt ggctgggcta 46980

aagctgtatt cttagcaaac gagcctgcag taaggagcag aattaatggg cagtaaattc 47040

atggcatctg catacaaccg tttcggtgag ccaaacccga aagcaaccaa atataaaagt 47100

ggggggagtg tgaaacgaga ctatcgtgat gaaaaagagg tagaaactgt agagactgtt 47160

ttagaaaata ctaaaattgt ccctgtccca caacaaaata tcggactgat ggctgcacaa 47220

tattttggtg tgcgatcagc attgtctcag gaagacgggg taacagtcac tgcaacatat 47280

ttcccatatt acgaccgcta cggtaatcta tccggcttta aaaagcgtga ctggacaatc 47340

cctaaagagc aaagaggcca tttctcagtt gtcggcatag taaaggccaa ctctcagatg 47400

tttggtcaaa agctgtgtgc atcctccaac aaccgtaaac agattaacgt ttgcgagggg 47460

gagggtgatg ttattgcagc atggcaagct gcatatcaga tacaagtaaa agggattctc 47520

accaatgcta aagccccagc aaaggtgaag cagtgggcta aagaagttca ggatgggatc 47580

gatgcagtta tcaatggcgg tgatctggca ggaaaaccct gcctgccttt tgttggaatt 47640

aactgcgggt gtgcaaacgc cgtagacact tttgcgaaca atgagaaatt tatccgtagc 47700

tacggaacag tggtccttgc aatggacaac gatgcagcta acgaggtaga gaagcaaaaa 47760

cacgttatca aaggtgttga ggccacacat aatatcgcag catttttgat ggctgataac 47820

gtttaccacg tggaatatcc aggtgaggta aacgaccctg atggtgtaaa agatattcgt 47880

gacatgctga aggctaaaaa gctggaagat attgtcaaca tgttccgtca cccagtcaaa 47940

tatgtgcctg atgctgtatc tgatttagaa gacttctcca ttgaatctct tcgtaagaag 48000

acttccaatg gcgtggatat cagtgcggag ttccctaaat tgcagaagat gctgaaaggg 48060

ctccacaaag gcaccttgat gatgctaaca ggtccatctg gcggtggtaa aacaactgta 48120

gccaagaagc tggaacactg cattgcaaga tacttgatgg atccaacatg ccctaaagcc 48180

gatgattatg aagaggatga tcgcctgtgt ctgattcacc ttgaagagga tcctgaggaa 48240

gcaatcaact ctctgtacgc taaccagctg gggtttgatg ttaaagagtt tgttgaagac 48300

cccagtcagt tcctgaccga ccaggagcac gctgagattc atcaatcttg ggccaaggcc 48360

ggaaaaatca aagtgttcaa gcatttcggc tccatccctg tggatgagct gatcactaag 48420

cttaaacaaa tggtgtgcct gtatcactgc cgttacattg tactggatca cttgtctatg 48480

gtgatttcag ggcttaatgt taaggatgag cgcaaagaac ttgacatggc tatgactcaa 48540

ctagccgcgt tctgtaagca gtttaacgtg tttatcctgg tgattgctca cctgaaacgt 48600

acagagattg tacctcctaa ggataaggat gggaatccgt tgcctttctg gtaccctgta 48660

cgaaaagaga accttcgtgg cagtgccgga ttggagcaac ttagctgggt agttgttggg 48720

gtagaggcag aagagatggt ggatcgttcc cgtggacgtg tccgtctagt aggtcttaaa 48780

aaccgcccag caaaaacgtt aggtattgcg gacactttag ttatggatcc ccacacaggc 48840

aagttccacg atgccagcaa ttggtattgg gataaagaaa tgcagatgtt tacagacggg 48900

gaaggtgggg aggttgtttg gagacctcaa gctatgtttg aggatcagga gcatgcagtt 48960

gtagaaacac ctgttggcaa ggttgtggct gataaggtta taactcctgc tgttaaccct 49020

gttgacacac aggaagacct tcagtatgat gatccgtcct ctaaccacgt tcctggtttt 49080

gaagacgacg aaacaccttt ttaatgttta accaagagtg ggaggggtag tacaaccgcc 49140

tcccctttaa ggagaactgt atatgagtat gtggtctttc gatctggagg cctcgggctt 49200

gctggaagat ccagatcttt attatcactg cggtttgttt aaagagctga ataagaaccg 49260

ttttatgctg ttccttcctc taaatgaccg cacacactac tctgaagagg atgtagaaaa 49320

ggctaagaac tttattctgg caaagaaaac tctttataaa gattttgagg ctcgcatagc 49380

agatttttca gagttggaag gctggcttac aggcaattca gactggtcac ctacggcttt 49440

gaactgtcat aactgctaca gctatgactt tatgctgatg gaaagattat ctggtattca 49500

ttttgatatg ttccgtgatc ctaaatgcat ggggactatc aacgaccacc aagttaatct 49560

atttgatacg ttggcgatga gtcgaatctt gtggcctgac cgccctttgc caaaaggttg 49620

tccggacgca gtattcaatc cagttaccaa gaaaatgcaa cctgttgggc ctcatggcct 49680

tatggcatgg ggctatgccc ttggtaacca gaaagttcaa atcgatgact ggcgtgagct 49740

tccgctgtgg aagtatgtgg atcgtgtgtt cgaggacgtt atcatccagg agttgctctg 49800

gaaagagttg gtagcggaat caaaaggtgt attctacggc aaatctgaca tgcataactt 49860

tatgtatgat cctgccaaag agaaaccgaa agggttcaag aagatcacct ggaagaacgc 49920

attacgccga ggcatgctgc agcacttcct gatggagctg caggcccgtc agggtgtgta 49980

ttttgacata gatggagcta ttgctttacg tgaccgctgc gatgcgtgga tgaaagaaat 50040

cgctgatcgt gttgaaccac aactaccttt aaaagagctg tcaatgtctc agcggcctaa 50100

gtttcctgaa aagccgttta accaggatgg gacaatctcc aataatggtt ggaaatggtt 50160

aaaagacaag cttggctatc ctgtggacat gagtgcttta gagttcaaag ccccgcctaa 50220

gagagcattt acctccacag gagatgtcag taagatgggg ataaaatggt gtgaagagat 50280

gggctgcaaa gacccagata agatggctga tttcttgaga ggctatatta agggcacctc 50340

cacaccacaa cctttaccta aagagttaat ggatcaggcg ataagtgatc tacagcagaa 50400

acgaatgccg gactgcaaga tacctatgaa aatcagcaat caggacgaca ttaagcgata 50460

cctgatcagt gctggttggc ttccgacaat gtggagaact aaggatgtaa ctaaagacag 50520

caagaagaaa gctctgcctg atgctgatgt tgatgcacga gtatatgcat atatggacga 50580

gcttttagag tcagagtatt gtgacctaat tataaacttc tggaacaaaa ctgatgctaa 50640

gtttcagaca accgtgcaca agttccgaag cttccctaac tctgaaagga ttaaaaagga 50700

agtgtttggg aagatacgta gaaaagccag agcgcttatt acttctccgc aactgaaaga 50760

tacgtttgga cacttatgtc ctaatcttga aaaattgaac ggagagatgg ctaaagatat 50820

cgttttgtgg ttatcattgc gaaaccgtag atcggtactt gatccaatca aggaggataa 50880

ggttgatact ggtcttctta atcacccacg tttgaagatt gaccataagc ttcctgctaa 50940

aagttctgga ttaacaaaca cttccagaca gaaacacagt atttgtgcaa acatgcctaa 51000

accatctcct aaagtggtta tgggtaaaga gatgcgtagt ctgtggggtg ttcctccagg 51060

atactttgag atcggtattg acgggtctaa cttagagcag cttatcgggg cttggggtgc 51120

ctttgagttt gacaacggcc tgtattatga tgttgtaagt aacggggacg cacaccaaaa 51180

caatgctgag gcatatacaa aagtagcagg cagagaggtg tctcgtaacg acggtaaacc 51240

tatcacgtat ggggttatgt acggggcaca aaaagataaa gttgcagaca tgctggatat 51300

ctctccagag ttggggcaaa gggtaatcga tgcgctttgg gatgccaacc ccggtcttaa 51360

ggggcgtaaa gaggatctgg agaaattctg ggaagcaact ggtaagaagt ttatctactc 51420

ttttgatggt catgccatct ggacccgatc taaacactct ttacttaatg cataccaaca 51480

aaatggcggt gcatcgttgt gtgatttagt tgggatactc atgcaccacc aaatggtgaa 51540

gcgtgggtgg tacgatgaag gtgttcgtcg tattatttat taccatgatg agtaccaact 51600

tcaagtacct gacacaccga agtataagac tgtgtacacc ttcgacacaa tcgaagaact 51660

ggaagcattt aaggcagagc aggaggccaa acttcatgtg ttcgacggtc ataagtacaa 51720

aaaggcacgc aaggatgagg acggaaacga agtggttgac agagacggaa acacggtgta 51780

cgatcctatc ctgaacgatg acggaaagct tgagcttatc tggagccctg tgggtgaaat 51840

ggtggtgcat tgcttctggc aagcttctaa aatgatggga gtcccgttcc agatcacagg 51900

tgaatacttg tgtggacgca actggggcga ttgtcactaa tgtaaactat caacacacgg 51960

aagtgttgtt agacagagag gagactttta tggtagtaga accgacaaaa acatttacct 52020

ccatgactat tcctgttatt atgcaggtag agtgcgttca ggtgtcccaa gtgtggcccg 52080

gtactaaaat tccaacaggg cccgatttct tccaggttgg aaaggtgtac aatgtatgga 52140

aggcaccgga ttgttaccct ttcattaaag atgaccgtgg aatgttatgg tatctgggaa 52200

atgatttgac actgcatctt ggaaatgcta ttattgcagt gttccggatc aacaagaagg 52260

aggcagatca atgacaactt tggttaaagc gacgtttgta gaagaaaaga tgacagacct 52320

atcattcttt gaagaaggta aggtctacaa ggtttactac gataaggatc gcaggaacaa 52380

tgtgatcgaa gacgaggaag gtatttcctg gtatatctcc catctggcaa atggtgagta 52440

tcatatctat ggaaccaccc tgcttgccaa gtttgtagct gttgaagagg atctatgaaa 52500

gtaaagtatc tggctgaagg tatgagtagg tattttaccc tcggagagga gtattggccc 52560

tttacggaaa atggagatgt atacgtgaag gatgaccaag gtgtgccgtg gtttctgcac 52620

cataatgggt tggggtattc catctactgt ggtgcagtga acaacacaac ccttgcagag 52680

tttgtctaga ggaggcagaa tgttagtaaa aggtacccac acagtcaagc aacgtattga 52740

agttgagatc gatgattgtg agctgaagca tattttgcgg tcatgctcca acgttttgct 52800

ccttagtatt ctccaggagc gagcggagaa cgagttctta aatgcactgg caggccgtcc 52860

tggggacttc tgtgtgcgta aacgtgaaga tcgtaagtca tggttgtggg aaatcgatgc 52920

cgaatgggat taccacaaaa atgagggtat agatgagcct attcgtgaac tgacggacaa 52980

agagattcag atgtacaatg aagtttgcca gtgggcaaaa agtcaacaga cttatctcga 53040

tccttggtgg atgattgaaa atagtcgttg acaacaggtt ggggtagatg taagataccc 53100

cacacaaaca gagcgttgat ttttaaataa ctaaggagac tgccaagatg aaaattactt 53160

tgaagccaga atgcgttgca aatgaccgag cttttaagga ttgcacagtc ggtgcaattt 53220

atgaagcaga gttttgtgag aaccctctgg taaaagccct tctggaagaa ggggcccctt 53280

tatcacaaga agagattgat gagctggaaa actcagccca gtgccttacc tttaaagatg 53340

atgaaggaga ctctgtctgg tgcactgtta atgaagtagt ttttgtaaaa cctgaacatt 53400

aatacttgac aacagtgatg gggcctaata taatggccct atcaaaacga aaggggacca 53460

cagcaatgac caaacaatat caacgaatta aagccaaggt taagccagac tatctggaaa 53520

aggactctgc ttttcgggac tgcacagctg acaaagttta cacaggggcc gtcctgttga 53580

acgacgaatt tgaagtggat gtgctgggta aagaggctac tatcaaggac gaccagggag 53640

attatgtttg ctgcaccaca cgagagctag aaatcttaga atacttcggt gaaatcttca 53700

cctctcttga tgattactta gatgatgagg cagagtgatg aaaaacattt gggatatcaa 53760

tatctttgaa gcctctgagc ttgacggtgc cgatggggtt attatagttt ctgatggaaa 53820

ggcaacactg acctctggac tgacctatga ggacactacc tacgcattgg gcgatcttgc 53880

cttgttgaaa tcaacaggcg actctctgga agaacgtctg caaggatttg cctttaaaga 53940

aggcacaaac accacactga tgtgcactac acgcactgtt cagttgggca acggggtata 54000

tatggtcatt gcagtgtgcc ccaaattcag caacattaat aaagatgatg ctgacgctgt 54060

caacgacgtg gttgtcctat tcgtgggtga cgctatggca gatctgatga atgcaggaga 54120

agcagcaaat gaagactgaa aacattacac cagaaggatt cctgaaagta taccgtgaac 54180

tattgtatac cacagaggat agtgttctag agtacccaga atatcttttg ctgtataagt 54240

atctgcaaga agctggtgaa aatcttccaa ttagcgcaga aggtggtcca aactgggtct 54300

acagtgcatg gagaaaggtt gacattctcc cagatcccgg taaagatatg gtagtttggc 54360

agcttatcaa caacatcggg attccaatct ttgatgaagc tgttaaaatc tattttgaac 54420

ttgacgatta atatcaggag accttaagat ggctgaagta gttgctcgtg caggacagaa 54480

agtgcgttgt gttgaagtgg gttttggtgg caaagggtgt caaaacttga ctcaaggtaa 54540

ggaatacact gttgtttctg gcggtggtga cgaaagtatt gtgttcgatg accatattct 54600

ggatgttgaa aactttgaaa taattgatga tgatggtgat ccgatcacct gcctgctgaa 54660

cgggagttgg ggtactttcg aagtaattgg ctaacaaata cctgttgtaa tttatagaag 54720

gagatgctaa aatggtgtct cctttatgtt gttcaaataa aatcggagat tccataatgg 54780

aacaaatgaa cgatcaagcg tcggttgcga tgaacgagca agaacaacct caagaatttt 54840

tcttagggtt aagcaaagat caggtgtctc tcctgctagt gctctcttct ttcatgggca 54900

gacttcagga gctcacacaa caaccagagg ctgaacaatt atttcaggct ctgcgcgaag 54960

aagaaaaata tcgtcgtttg gattatgcct tagggtgggt agctcagcaa cgagatatgc 55020

taatggccct ttctgggacg acaagttttg caatgggcct gttggctcaa gtgcaggaca 55080

aattaattca agcagggttt gcacacacag caaacaccaa cgaagaacaa ggagataaat 55140

aatgagcgtt attcaacaat ttgccaaccc gatgaatccg cgtgaaactg ttttactggc 55200

tcacggtatg attaaacgtg tcagcatcaa gcctatcaaa ggtgctgatc ctacaacagg 55260

tattaaaacc actgtgtggc aaggtaagaa aatcgagtcc agtcacacca tctctttact 55320

gatttctgaa ctggattctt ctgataacct acttcaaggt ggtgaagaag tttggatcag 55380

catgggtgat aaattgctca aaccaggtca tcaagattcc gtctctgtta aaattgatga 55440

caagtgggaa acagtaacgc caggatggat cgtaaatatt cctttgaagg caaatgagca 55500

taacggtaag acctactaca aaggctcatt ggctaaaatg gttaagctgg gggcaggaga 55560

ggctccagca gctccacagc aacaaaaaca gcaacaaggc acacaatcac aaggccagaa 55620

gcagaaacct cagcagaatg ccaatcagga cgttttacgc atctatggtg atgtgactca 55680

gattgttggt aacgtggtta ctgtcaatga ccacaagatt ggcgaaggtg ctatggttgt 55740

ctccgatgag cagcttaaag acctggtggt tggcggtcgt gttgcagcta ttgttgacaa 55800

agacaccggg aatattatca gtggcttcaa ggcctacccg cctaaagcag agaacggtac 55860

acaaggttct ggtggtaagc gtaaatcctc ttacgacccc atcggtgtag agacagggca 55920

cagtatcaat gcgttgcaga tcatcctgga tcgcgggttt aagattgaca gccctttaga 55980

tgtggctaaa acactgcacg tggtcaccat cgagcttaag aaagagtacg ctcaagccac 56040

taaccgaact gaagatgaag taggtgccag tgttggtaat gcggtattga acgcttgtcg 56100

ccgtattgac aagaaaacga aagttgatga ttttgcagta gcaattactg cagaggcaaa 56160

agatgtcttg actaatcttg ctgaaccgtt gtatgattgg atcaagaacg gtgctggagc 56220

cactgaccat caagagcctc aagctgccaa acaggaagcc gccccacagc aaaacactgt 56280

agacgctcct cctgtagatg atgcggaaga cgacccgatg gatcagaatc cggcacagta 56340

cgcctttgat gatgacatac ctttttaaca tctagttaac atagccctac ccgaaagggt 56400

ggggcatttt tgtatagaga ggactgaaaa atgacattag aagatatcat tgttaaacct 56460

gagaactatg accaatacaa tctatcaact caatctgtag atcttggatg cgccactgtc 56520

agtgcgtggc tggttaacgg aaaagagctg gataaatgct tagaagctca catgactgtt 56580

aacaagttcc tggatgaaaa tactcactgg ttggaaggtg caggtgtata cgcttcgtgg 56640

ctggagagca tgggatttga ctaccagtct gaagaagggt ggtggagcta tttagctatc 56700

tgtccagaga cgttgaaatg cttcatgaaa tattctgaag atttggagta ccgggcggtg 56760

gttgatagtg ctattgcccg ttatagccgc aaaccttttt cccacgacat taaaactgtg 56820

gaagaatttg ttgaagtttt ctgttgacac aggttggaag gtgtgcaatt ataaccttgc 56880

agagctggag ggtgtgatga acgaagaaaa ctgggatgtg taactaacag gaggccactg 56940

cccatgattt atcgaatccg tgtgagagaa gatgcaccag gctgttactt tgactggcgt 57000

gggtggtggt ttctccaccc aatggatgga ctcacagata gaatgctgga tgctgccctt 57060

tacacagata ttgacataga gtataatctt tacttgaagg atggtatcga taacggcctg 57120

tttgaagcaa ttcctgtgta agagactatg atagccatgt tttatgatct tcctgtagca 57180

ccaccacctg ttgaacccgg agaactaaga tgatctatga aatgccaaaa ctgattcgcg 57240

acgatgccca aaacgcccac accattatta tgggcaaacg catggatatt atttgtcata 57300

aagatttctg ggaaggaagt tacttcacag agtattttgg taaactggac aatggtctgc 57360

tgatccagtt ctacaccgat gcggaaaaca acattcactg ggagttcgaa actgaagata 57420

tccataaact tttcaccttc ctcggtatta aggttgaggc caagtttgaa gaaggtgagt 57480

gcgacgattg tggattctat gaggtaacag atttctatct cccgagtggt gagaaccttt 57540

actatgaaag ccacttcggt aactccaata tgccacagtg ctggactgag ttttttgaaa 57600

ttgtggaaga ggagctggca tatcgtgatt attgatactg tagagggaga tttaatctcc 57660

ctttttaaga gtggtgctgg tcacctgatc cacggttgta actgcttcca cacaatgggc 57720

aagggtgtag caaaacagat tgcccgtgag ttccctcaag cattggcagc tgacaaaacg 57780

actacctacg gttgcctaag taagctaggt gcgttctctc ggtgggagca cttatctccg 57840

aaaggtattg tatacggaat caatctgtac actcagttct acccagggcc taatgctgaa 57900

tatttctcaa ttctgaaagg ctttgagcga gtcaatgaga tatttaaggg atcgacttta 57960

ccattctaca tccctaagat tgggtgcggc ataggcggct tgaagtgggg acacgtggag 58020

gacgttatta accttgccac ttttgatata gatattatcg tggtggagta caaagagtgt 58080

tgacagcagg attttgaggg ggtatagtca ccacaacgac taagacaggg gaacactatg 58140

gagactaaaa tcttaattct agctgtgata gatacaacag aaggggctat atttgaatct 58200

gcaggagggt atttctacct gccctatgga aaaagaactt ccgagccgag taaagattca 58260

atcctcaaag tgttcccgtg tgctttctgg ctggagattc tggtagagga ttgcagagga 58320

ttggagagac aattatgaga aacaagaaag agctttactc tggtgagtgg atcaacgatc 58380

aacaggtcgg agaggtaacc tgcttccggt gctttactcg gtacaatctt aaaactgcac 58440

caaaaagtgt tccaaagact gatggttatt acataaaaga acctcattgc ccaaaatgca 58500

aatgtcgatt atactggagt aacctgtaaa agattgacat ctgaaaactg cggtgtataa 58560

tacaaacata gactagcaga gagggacaaa ttatggtaat ttttaataga gacggtatca 58620

tagttagaca gcacccattt cttgaatact atcaaattga gcaatggtgt tacggagatt 58680

gcagtcactc ctatgggcaa agttggggat atcacacagt ttttgaatca acagatcttg 58740

ataaagtaaa acagaaagta ttagaacttc ttaacaacaa gtgagtatca tgccatggta 58800

acattagaaa gacgtaaagc tatcgccgcc acactggcag acatcttttg ggattgcaat 58860

ttaaaagata cccgatattg cggagcatct gtagatgtat tattcccacg gcccaccatt 58920

gttgcaatgg gtagcctact gagcgtagtt gcaggtgcat gggataagga aggcggtttt 58980

tatatctggc agcctgacta cgggacctgg aaagaagtaa ggggtgctga tgactttggg 59040

cctgcagttg ctaagatgga tggctgggaa atttatgttg ggaacgacat gaagctgaaa 59100

ggcgagattg tttactggtt accttaagaa agtgcaggag ataaagtatg aacaaagggt 59160

ggacatttgt agacggcgtt aaaaacatgc ctctcggaaa gtatatcgta gccatggctg 59220

acgagaatgg taatgtagat ttcggggctt gcgaggttgt tcttgcaggg tctggtaact 59280

atgtggacaa aactgggata attaacggtc agttctactt tgatcacaac cctgttgtag 59340

cgtatatggc tatgccagag ttcaagttcc caaaggaggg tgccgatgtt taagcttaaa 59400

tctgcatgga acattgctgt aggtgatgag atccgcattc cgggtgggaa gaccgttatg 59460

aagatatctc gtattgaata cgaaggtgac cgtgtattcc acatctttgc agaagatggt 59520

cgagaaattt atatacaggc aggatcacac atctatattc gtaaagggga tgacaaatga 59580

cagagatcat catttcaact gcagggtatg tattcctgac aatcattgcg ctgatcatct 59640

tggtggtggt gtatggattt gttatcctgc ctattcttga gacatttagt ttgtgtcggt 59700

gttggagaaa ggcttacgga ccacagccgt ggagagactg gccccacctg ttacgcatag 59760

cgttctctta tgcctatgat cactccagta gtgcatgcat aaccgggtat cacgcccaca 59820

actggaactg ggaaggactg gggagatgga gtgttataaa aactatacct ctacgcatgt 59880

acaagcctaa aaacaaagat agttgatact tgaaaatccc tggcacatca aacataagga 59940

ggatttctaa aatggatttc ctccttttga tattttacct ggccggatat tttaaaattc 60000

ttagaaaatc agaaaatgga tcgaaaacta aaatggattt tttcaagaca ggcacgataa 60060

gctgtgctga tcatcctatc gttcgtaccg tacttcaaat caccgttcca gattttccgc 60120

acacactctc ccccatctac cagagctttc ccaactcacc tctgaccttt tatagcaata 60180

agtccattat tatcaagaag tttctgcaca tctatctcag tactcgatct gtttttcgtt 60240

tcaaagattg aattttctga aaatttttct cgataaattt ctcagtactc gatctgtttt 60300

tcgttttctg aatcagattt tctggaaatc gttccggcgc tcgacctgaa atttttgggg 60360

acgaaatcga tttttcgttt tcagaaatgg atttttaaaa tttctatatg ggaatatttt 60420

aggaggtatt attttatata gaattgtcac ggtaggtaat tagcatgcta actaaattta 60480

tgaaatgcga aaagttctca tttagaattt tgaatgataa tcattctcat ttgcagaata 60540

acgggcaccc ccggtgattg tctcccggcc cccactgccc cggcttgtta ttatagtatc 60600

acacaggaca aagaaagcaa gaagtttttg cataaatttt tagacgataa tgataatcgt 60660

tctcatttca tgcagcgccc atccccggtg gttgtctccc ccgtcaccgg tgcctgccgt 60720

cctttcgata tgtatattgt gccacctggc ggggccgctg tcaacacttt tcaaaaaatt 60780

ttttctaacc tgatgcgcta accgctgcaa cctggcgggg ccagcctggc aggacctaac 60840

tgaccaggta ggggagaatg aaggcttgca aacctggtgg gcctatgcta taggataggt 60900

aacaattgaa gaggaggtac aaaaaatgaa aggtaatagg atccccctgg aaaatgtagc 60960

tattaatgac gctgaggcta tcaccctggt tgaaaaggct atggcgggag cgttggaaca 61020

taaagaagag tacgaaaaaa tgatggttaa cctgtcaggg gcggcggctg gagaattcta 61080

cccaaaaata attttaactg atggttgaca gctcgtacag ttcttgttat tcttatcctc 61140

gcaaggtgaa caacaccaca ctaattaaca gagaggataa aaaatcatga acgcctacgg 61200

aatcaaagta cgtcgcagca ctcgccaggt ggtcaccgat caaatggttt gccgggtgga 61260

tgtaaccatc aacaacgggc aaggccttta taaattccgt cgcggtcaga gctatacccg 61320

gcaacagctt aaccgttgcg ccctgacaaa aggccagatc aaccactggt ttaataaaaa 61380

ataagtattg acatatagtc cgacaccaaa tacactaaaa tcacaaaaga cactaagagg 61440

ataaaatcat gaaaaccact acctttaatt ttgataatgc ggacttttct ttttctgatg 61500

tcaagaactg ccgccgtgat cgtggatttt acaaggctta taatctggtg ggcctggacg 61560

atgacaataa tttgcacgaa gtagctgaga tccggtatta tgccgccgcc agcgggaaca 61620

ctcattactg cgttttttgg atacacgaca acaagaacaa taagtacggc tatactggcg 61680

gcaaggcgag cggtcacggt tatgataaag ctaacgcagc acttgagagc gcattaaatg 61740

ctatggggat cactgccgac ggctggatct acgaagaaga gtttttgtcg gcacttgctg 61800

accacctggg ctataaaaag cacattgtaa catgcgccca cgaataaaaa attgttgaca 61860

cgctgcaaaa ttcaagtaaa ctgggcacaa aaggcgggaa tgtttcccgc caacacaaag 61920

gaaaaaagat catgaataag aacgtacgcc gcatcatgaa cgagttggca aacgatgaaa 61980

aaattattga catgcatttg atcaacattt gccgccgttg tgtaacatat aacaaccgcg 62040

ccgcaaaagc gcgtgagatg aacgcaaaag gcaccttaaa gccattccag gtggtggtaa 62100

aggtcattaa agatgtaact aatacggcaa accaggtaca ggagagcacc aacgaaagta 62160

ccaatattga tccggcttac atcatgaaac ggatccggac ctgcaagaac aaagagcaag 62220

tgtataatgt catagactgg ttggattcta tgggcgacgt ggtgtacaat aggttttctg 62280

cagctatcga gatccagctt gacaggttag acgggaaagt actgaaaact tattaagttt 62340

atttttagat ggcctttact ccagggccat caataaagta aactagtctc aactaagcga 62400

aaaaaggggg ctttaagatg agcactacac gccaatacac aaaggtaact attatcgttt 62460

gcgttgtcgg ttacatcctg gcgcaaatgt taatgagtag cgccaacagt tttgcaaaat 62520

ttttcttaaa tttttaattg acaagctaac ccaaatcggt atacttaaac acgtaaacgg 62580

gaaaacttcc cagaacacac aaaaggagaa aacaccatga ctactatgac cgccgcagaa 62640

aaagctatgc agatcgccgc gctgaaaaaa cagatcgcag accttgagaa caagatcgat 62700

aactttgaaa tcgacgagga caagtacgac gaaaactatg atgaatggtt agacgagatc 62760

tacggcgaaa ttatgatcgg aaacatttct tttcttccgt cgcgtatcct caaggagtta 62820

gatcctatcg cttaccgctg cggatttagc gactacatcg acagcctgga tattgaggac 62880

gacgaagagt accaagaatt gcagacagaa ctcgaagaag tgaaagaaga gcttgcaaag 62940

ctggaagaaa acgagtaaga ttatttttag atggccttga ctccagggcc atcaataaag 63000

taaactttac actgttaaca ctgaactaat gaggagttaa aaccatggca gcactaatca 63060

ccaaatatta tgcgcccacc aacaccaaaa gcgcccgtat catggtaaaa ggctttgggc 63120

gtaaaaaggt ctacacctgg gactattccc tagacatgga agacaactac accaacgcga 63180

tgaccgactt tattaatgat ctgaataaac agatcttgac cgactacaaa gtgaccgata 63240

ttgcttacat aggtgaaggc gggatctgtt ttgtaggcat tgtgaaatag ttattttttc 63300

ggtggtcctg gctgcagggc catctataaa gataatttat taacaccatg aaaggagaaa 63360

gtacacatga tcagcggggc cagttttatt atcggcgcta ttgctacggc tttaattgta 63420

aacaagatcc aggataaaaa ggaaagagga agtaccgccc ttgcagaata tccattttgg 63480

acattcaacg ataacgaaga ttatattata aagtataaaa ataaagaagg tgaccaagta 63540

acaccgcgac cgatcccggc ttacaaaatg caggaaacac tataccgcct ggaggcgggg 63600

ggcgcaaaaa ttgtagactt ttggggatac gaccacacag accaggaatt tataaagcgc 63660

cgtatcaggg ctattcaggc aaatattgaa gttagtaagg ggggaatata tgaaggttaa 63720

ttttgagaag attataaagc gctctcatga ttccgaagag ttgccagaac gccgacagcg 63780

taacaataaa ctgaataagc caaaaaggtt tgggaagatc tggcgtaacc attatgatcg 63840

taaattgcct ggcctacttt tggaacgtga acgacaggcg gaacggtacc gccataaaaa 63900

caaataacaa ccaacgcccc gccattgtgc ggggcttttt atttctatcc tacgaatagc 63960

gcccctggtt ggccctgtaa gcgccaaaaa ggcccccacc tatgcaatcg ccccccttta 64020

acctgaaaac atcgcccagg gccgttttat ggcgttaaaa atatcattga catcgtcaac 64080

aggttagcta tactaaccac atcaaagaga ggaaggagta aacgccatgc gtaaattaaa 64140

aaattttgat aaagttgatg ttatcctgaa taatttttac tactgcaaca acaaggctat 64200

ttggtggcgt gatagttcca ccgataaagt caaggcgcgc tttctccaag ggcactggca 64260

aggccgccca gtgattaaaa tcatggggca attggtagcg tttaaaaatt gccgccgtgt 64320

tgatgacgtt gaggagattt taaacatttt gcgccacaat aatttaaaaa atagtgttga 64380

caagtaaatc tcaataagta taattaaaca cacaggcggg aaaacttccc gccactaaca 64440

aagaaaacag gagaaaatat catgaccacc gttaacatta ccacaggtta caatttatct 64500

ttcgacgtgc tgaccgacga acaaaaagaa aaagccattg aaatggtacg cgaaagagaa 64560

gaacacagcg gggataactt ttttgcagaa agtgtaattg aacactataa agaatatgtc 64620

tttccagagt acggccttga agataccgaa gtacgttggt ccggcttttg gtgtcaaggc 64680

gatggagcgt caatcagtgc tgaaaatgtg gatttagaaa aattcttgcg taaagtcaag 64740

gccctgacca agttccgatc aattcgtcac ttgttcggta caatgcgcga tggcgaacta 64800

tcagccagtg ttgaacgtat cccgtttagc cattatcacc acgaaaacac aataggaggg 64860

tgcatcgata ccaattggct agacctgacc accaagcaag aggacaaagt tgaagagctg 64920

gaagagctga tcaccgagac ggtacgggag ctatcccgca aggtttacgt agacctggag 64980

gaggcctatc tagaacagtt tactaccgaa aacctgatcg gccttattga ggccaatgat 65040

tggcggtttg atgtcgatga atatggtgag atgacttttt cataaataaa gtgttgcaat 65100

aacctaaaaa cctgctatat taaaactcaa ggggggcaaa cgtcttgccc caacacaaaa 65160

caaagaggaa aacatcatga aaaacactac caaggctatt gacaccgtta tgtttaacct 65220

ggttacattc cgtgaagata tgaaagatct tcctcgtgaa acagtgtgcg accatatcga 65280

aaactatcgc aaactgatta aagagttgcc attaaaacga gatcagtacg cggctaacgc 65340

tatgctgaac gccatgatca aaaagatgat ccagtgtgat ttaaccctgg cttatgatta 65400

tgcaggcggt cgttttgcag tatacaatca gcccgtgcct ggtatgagca ccaccgaaga 65460

attgcacgcg ctcaatctaa aacaagacgt gtgggatagt atgcctcgcg tggtggtatg 65520

ggtagaaagc gggaagattt acggcgataa tttttaatca ggtaacaggc ggcatatttg 65580

ccgccttact gttaatcaga attgtaagag ggtacataga atggaattta ttattttggc 65640

cttattagtt gccttgttta acgcctggat cgctggcaag ataggtagat ccgccttttt 65700

atggtttatt atatctattt tcctgggagc ggttgcaact attgtattgg ttatccttgc 65760

aattgttgac aaggggaata atagaaaggg taaagtattt tcagacctgt acgacgctca 65820

aacgtttgtt aacgcctatt atagtgatct tccggtctcc ctgcgttgca gccttgcaca 65880

acaggttttc gatgaagcta aaaccagaga tcacgcccat gagatttgtt taactgaggc 65940

ggatcgctta tttgagagga atagaaaata tgtgggccaa ccgtttaaat aataccacgt 66000

taattaatcc ctttagtgaa gatttgaagc cagccgcctg gcgggatgga ttcaataacg 66060

aaagctttat tttcaggcct gatcttaagg aattttataa caatggatcg aacaacgaca 66120

gagataattt ttctcgtaat gtgcgtggcg atagtactcc tggggaccat tgtaaaccac 66180

gtcactacta acaggaggaa agggggagac gatgacaccc aaggataagc cccgcaccga 66240

taaggcgcgc cgtgttatgg tatggctgga gatcatcgcc cttgaaatat tgttaggcgt 66300

agggatgtat tttatcgctg tacgtacctg ataacggccc ccgatccctg ggggcctaac 66360

ttttttcaaa aaatttttct tccggggtgt tgcaatccgg tcctgaatat ggataataac 66420

aatcaacggg gagacactcc ccaccacaca gacaaagttc caaaaggaga aacaaaatga 66480

gcaacaatat ccgcgttatt aacttcaaaa aagaagaaaa aattgctgtg ctgattgacg 66540

gtcaagaaat cttcaagggc gattatgaag atcatcaaac ggcaattagt attacccgcg 66600

tgattggtta tcataatttc taccctcaag gcgctgaggc agcagtaaaa cgaattaaag 66660

agattgtagg cctgtaaagt ttatttttag atggccttga ctccagggcc atcaataaag 66720

taaatttaaa ccctgttaaa ataatcagtg caaaaaggag caatgatcat gtttataccc 66780

gccaataatc tttatttctt tgatggtcag atcgtagacg gcgtggaagt tgagatcact 66840

ggatgtcaag gcggccaggt aatgatcttc cgcaatagtg catttaacga tcaattcatt 66900

acaatgacca tggaagtcaa aaagctggac gataagaaga cactcattga ctattccgtg 66960

aaagatcatc cagactacca catgatctat ggtgatttgt gcattgttga ccgtgacaag 67020

gtgggcggca ttgtgatcgc tattgtgaac gatgttttct ctgaataatt ccggcaaatg 67080

atgcaaaata aggcccatgg cagggcctgc agcgatataa tcacataaag gctatgcggt 67140

tgcatggcct ttattatttt ggcgctacag cggcccacaa ggcgcttaaa aaatttttaa 67200

agaagtactt gcatcaagcc ctacaatatg gataatagaa cgtgtcaggg gggcggcccc 67260

caatcattaa ccggatagat aggagttaaa accgtggcta ttgttaccgt tggcgatgta 67320

gtagaaatgg aagcaaaata cttaaaagcg ggtatgcata tcctacgcaa cgatcttgaa 67380

ctggtcctga caatggttga ctataatccc ggccaggatt atccaagcat cgactacttt 67440

tttgagtatg ttgatgaaga attaaaagaa gatgaagctt atcggggtga attttgtaat 67500

ttcttttgcc ccgatgaggt tgtaaaagtt atctatcaac ctggcgtacc tttcccgttg 67560

cacgccatca ataaataagc ctggcggggc cgaaaaaggc ccctcctgaa actatgaaaa 67620

aataaagata ggtgaatcat catgatggaa caaattaaac gcgctgaggc tattaatggc 67680

aatgtgatct attttgtcag caaaaacgaa aaaggcgtac cctatgcggc ggtaatagta 67740

cagcctgacg gcgattattg gtgcactgtg tacggcgctt attcttttgg gcgtccagtg 67800

catgataaaa ttttgaaagt agcgaaccct accgggaaga atatcacggc agaaaaattt 67860

tttgaaaata gttgacatga ccatataaga ctgtagtata taaatcaaag ggggcaacaa 67920

gccccaccaa ccacaaagag gataaaaaat catggctatt attgcaaaga ctgagatcgt 67980

aggcttggat tctattctcc tcgcatatga ggaagctata gaaatgtatg tagtttccta 68040

tggtgaatat atccgtgaat ataaatctat tgatctggct tttgaatgtt atattcgcca 68100

ggttaataaa gcgtacgatg attatcgtta aaacagtttg acaacaaaca accatctaac 68160

aaagcataat acataaggcg gcagcgatgc cgccaacatg acagaatgaa aaacatcatt 68220

tacagattga caaaacgcaa cattatgatc gccaccgtga tcgcttgcct tttcactttc 68280

gtgagtaacg caacgtttaa cgccgtgggc ggggtgatgt ttaatgctgg cagcgtttca 68340

acagctaaca caatgatcga ttgtttgcaa cttttctttt atgttgcctt tcgtttaaaa 68400

atagcttgca agttagaccg ttttgattta ccttttattt gtggcggcgt gatcgctatc 68460

gtatgctaac agaggatcag aagaaaagtg tacgacatag ggcggggcaa taaggcaacg 68520

cccattaatg agggtggaat catgttagaa aaaattttca cggttgtagt agttggcatg 68580

gcattatcgg ttggagtggt tgcaactatg gctaacatga tcctatcttc ccacggtatc 68640

gggtaaggtg aaacaaatga tcacgaacaa aacaggcaaa acggaatttt tacgcggctt 68700

gcaaataggt aagcctgaga tctggtattg cactaaaaca ccacgtgacg aaatgaaaga 68760

ttttaccgcc acgctgaagc gggtaaagat atctatcacg cagaaaaaag ccttgatagt 68820

ggtggaaggt gagatccctc aagccgttgt tatagttgag cgtacagcat agtttaatct 68880

ataccgtacg ccccattaac taacaaagca taaacaggcg gcggtgaccg ccttaaacgc 68940

agcagaaaga ggatagacaa catgaaaaag atgatcgcag tggttgccct ggttattgga 69000

cttaatgcgc ctgtaatggc caaagagttg gcaaaagagg tagatctaac tgatatccat 69060

gccgtcaatc aaatcggtgt tacttatcgg ttatgccttg ccgcaaaaac tttccaggac 69120

ggcacaacag agtactacgg attcgagggc gtccggtgcg gtgagcttgc tcgcgacctt 69180

aaagaagatc cagacctgga ggatctgccg ataaccgtaa acgggaaaac ttacgtgaac 69240

cgttaaccat gagaaagata attattacca tggggatcat ggcggcaatg ttggcgatct 69300

ttccctttat ggttaggtat gtgctgatta atgttgtcct ggtagtcagc gatctggtgt 69360

tatatattaa ctcattgttt taggcgcgat aatgacagcg gaagaagtta aaaaagtact 69420

ggcggtactg cgttatcata atcattgcaa ggtgaagaat gatcctgatt atcttgattc 69480

atcccttgaa gaggatacac tacaggccat aagcaacatg caagaattgc ttaagcgtat 69540

gacagagaaa aaccgaccgg actatatagg cgaaaaagta taatctttat aaaccataaa 69600

acagggcttg taatggtcct gttttttgtt atacttgttt cacgggtggc acctggcgac 69660

ggggggagac aatcaccggt ggtgcctgtt atataaaaaa ctataaaaac cgttgacatc 69720

tgccaggtgg tggggtaaca tttatcttga aggggcaaaa gtgcccacac aacaaccaga 69780

agagaggaaa acgccgtgtc aagctggatt aaccaagaag tcgaatcacg ttttaatgct 69840

atttgggatc tcatcgaaaa agaggacgga acatcaagcc cctaccttaa taatcttgaa 69900

ttccattttg tggaagcgat gaacgcagaa catgatcacg aaggcggcgc ggatgagttc 69960

gctaaagggt atggcttcac caatgccaac gaaatgatcg aggcggttgt actccaggct 70020

gaggaagatt atcaattaaa aacacttgaa ttttgccgcc gtaagtaata agataatgac 70080

acgggccaaa aggcccacca aaccagaacc ccataaagag gtaaacatca tgaaacgtct 70140

tgatcagctc aaactagttc agtacatggc ctacaaagtt cttgaagtcc aaaaaggtag 70200

caaaaaagag gtttgcgaga aagcatataa gatctttacc cgtaaaaaag atgattggat 70260

gttacgccag accaaaaagg ctgacatctt ggggtacctg gaagaagaga tccggcttga 70320

agagcaagaa gagatccgcc gcagtgaggc agattatcag gcagcttggg aaagctggat 70380

gagcaacaaa taatacaaca agttacggtc agggggggca ataaagcccc ttgatcacat 70440

aaaaattatg ctatccctgg ttgacaggga tcggcagttt tgctatattc ttttttgaag 70500

ggaagttgta agaaaattaa ctaagaggta aaaacaccat gaacattaaa tttaataatc 70560

cggcatatag ctttgataac gctttttgtc acggtccggc ggcgaaagga ttcgaaatcg 70620

atggtaaact aattattgtc aatgttgacg gggaagaggt tgcagtatat ccggtggcag 70680

acgttaaaag ccgtctacaa tgccgcatgg atgttaccgc ctatcagctt gatccggtcc 70740

gcatagttgc cctggtcctg gaagaagaaa ttaaagcaat gcaccacagc aacaagttta 70800

ttggtgacgc tcaagataat atgaaggtat gccgccataa agttaatgaa gtgcttaaag 70860

aatatggctt taaagatctg atgatcaaac gtgaggaaaa attccgctac cgtaacgacc 70920

tggcaaatcg gatttttgaa atatacgaat cattataatt ttttaactga attatcaagg 70980

ggcttaggcc cctttctttt tgtctgctct tccccatacc ataaacggcc ctacctgaca 71040

agtggaataa ttgacgagcc atcaatcccg cacactgtac aaatatacat gtaaaatagc 71100

tattgcataa cctttcattg tgtggtagcg gtgctatttt tacccgtttt ctctcccaaa 71160

attgcacgta atgcagctat tgcacaaaat ttaacctgat gcacatcttc agtatactgt 71220

caacaattaa aaatctttat aagtcataca cttacacaaa ccaaaaagcg tgtcctacgt 71280

gaaaaatcgg gtgtaactat agccgacaga tctcagaatc cacgtacaat caccattcta 71340

tagcctgcaa agtatttatt ccaaccacag aataacggaa tagtcaatac ttgtataaaa 71400

taattattcg tgtaaactgc ataacataaa tcttctcatg tgtcaacatt tttcgtaaac 71460

agtccgaaat tgtcacgata ggttactata atcagagaaa tctttagtcg tctaatgttt 71520

actgtacata actatactga ctggaatcat tagatgacta agagaaagat aagaaataat 71580

taacaatgta attgttttat ctgcaacaat aaggcaacta tgcaaacgaa tggcatggcg 71640

cgacggagtc gcgacataat ataaaagcag taaccatcac ccccgtgata gtttgtctgc 71700

aatctattaa tcagatcata gttgccttgt atttagttaa tcatgacata acaagcaagg 71760

caactataga tcttctaata gttgccctgc ttttatatta tgtcatatat tattaacttg 71820

ttaattgtca gcttgtttac tgttaacttg tcaattgtca ccttgctaat tgttttcatg 71880

caaacgaatg tcatggctta tcatctctgt gtaaactgtc aatgtgtaaa cagtctacgc 71940

aggaacaatc tatacgtgaa caatttatac gtgaacagtg aaacatgaaa taataacagt 72000

cgttattgtg tcgaaagtgt cgtattgtgt gacattgtat cagacaagtt tttctcctct 72060

ttgtcaagtg ctgcatggaa aatttacaat ctggtatggt agcatttttg cggccccgtt 72120

tacatgcatg agtgtatgta taatccttgt tgtgtgggcg tttagacgtc tggacggtca 72180

gaagtccgca ggttaatatc taatctattg gtcgctaact atatccacca caaataataa 72240

gaaatacaat tgttatattc gtaaacagta acccatgtta tcaatccctg tatgtatcgc 72300

cagtggtgcg cataatgggt attatgttaa attgcccgta atgataacga ttctcacaat 72360

gataagtatt ctcattcatc cacacaaaat agcttgcaaa tgttgtgcca ctgtgtgaac 72420

ggcctcgcct attaatttgc cccactgcct cgatatttga gaattattat aagaatgaga 72480

agtattccca tttcgtccgc ctggcagcac tccagtcgct accggctcct gacagggcag 72540

cactccagta ggtcaggtca aaatcgtggc agaactggac ctgttcagat atttctggct 72600

gactcttcag gcctgtgtgg gaaaagccaa cctctccggc ggcggggagg gatcaattaa 72660

aaaatttacg tggtgtatga agcgctcttc aggcaaggtg tgtattatgg gaggaatcac 72720

tcttcaggtg atgatatttg tgggtgtgat atttgctgga aagccagata tgacgggact 72780

cccggcgggg caggggtagt acaggaaggg aaaatatggg gatgtagttt gggaaattga 72840

tcagacaggt ggagtatagt atatgttaca ggtatgttaa ataggtaggt tgaatatctg 72900

taagatttgt gtgtaaaatc aaagacttaa gtggtgttgt ggtattttct ataaataatt 72960

caagtttaat aggtaatatt tgtctctatc cactttgtgt ggtaaccaga ttcagatgaa 73020

attggtttac aagtgtatac ataatataca aattaaagat acaagaaacc cccctccccc 73080

ttcggctacc attcaccttt tcctctgact ggtacttaaa cacttattca gactcttcag 73140

gttatcaccc atataccata tggcagtgac tggtgtcctc cggcccctct ttcgtcaccc 73200

gtccgcttcg ccatgctaat gccatagcta actgttcggt tgttcaccct gttcactcag 73260

gtgtatctcc ataccccttc aaaccgaaga gaacagaaaa cccgaattac tcggtaaaag 73320

aaaagagaag aacttaattt tatcaggata gacaagaatg tcaaggcatg actgctcact 73380

aaacaagcaa acagtcatgt gtgggaggta agtgattgtg gtggtaggga atttagttga 73440

aagatgtgtt ataggtgctt ccagcacttt agccgggcag ctaattgtgt gtcagtccag 73500

cggttcatcc tctacttcac tgaaatcaat gttcatggtc tcttcccaac catcgatctc 73560

agcacaagag tggagttctt tagggtttac tcccaaagcc tcagcaacag agtctatatt 73620

taacacctct ggcaggtatg attcatcaaa ttcaaggtaa atcaggccac gggtgttgtc 73680

agctccacca aatatttcta tcttcataaa atctcctctg gaaatgagag tgtgaacagg 73740

gttatacgtt ccaatgcact ggaatttata tgcgtttgcc tcttcaggcc atgctttagc 73800

atgataaact tatgtgggct cggtgcttca tagtctacca cctctgaact cttcgtttgc 73860

atgtgtgttg gtcatgttcc acctgtgagt attccggcaa cagccgctat ggacgtgatg 73920

atcatcagga tcaccaccaa aacctctgac aatgttttca ttgtgtattc catattctcc 73980

aaacgatgtg tggatactat cacaggagcg gtaatgtgtc aacagaaacg ccgcgagggt 74040

ttggttaaaa tccgagcgta tggtgatttg ggtcaaaacg gacgtgcgtt ctcgctacgc 74100

tcgaaataga gagttgttta caggccgccc ttaaacgctc tgtaataaga ttatccatct 74160

aaggtatgca atcgtgcacc agataaagaa aaggacgcca cagagcgtcc taggtgcctt 74220

aaatttgata ttttgctata cccatgaggg cttgtccagg ttggagtaac tgaaaggggt 74280

caaaaggtga ctgtcaaaat ctttcaaact gtccagatct ttttcctcgt cctggcacca 74340

tctgtgcctg catttattgt tgtagtgcat ggaatgcata gcgttctgca acttaatagg 74400

cacaagtgat tcaggagtag atagagttgt tgggtatgcc agaactatct caaacaatgg 74460

aaacacatcg aaactgaagt ctatttctgc tgaggttgct ggcatatagg agtatttagc 74520

tgtcgttgta gacataattc acctcacaca ggcttgataa tacctttacc gaataactct 74580

tcgtatctaa tcttatccgg atcccactcc tggataattt cgaagacaat atctaaccca 74640

gggataatca cttctccagt gtcaagtttg gcaagaacaa aaccagaagg ctgaatggtt 74700

tcccagttgc gacgattgga atagaagacc tcaaaaatat caccgacctc gatctgttga 74760

tcaagatgtt caagattctc tttcatacgg acgcagcgga tttttcgtgc aggccataga 74820

ccacctgcat cacgttcatt caactctttt acagtgatcg gtatacccat ctcaaatctc 74880

cttcatagat agtaaaaaga ggggcccaag cccctctctt tgtcattatt ctacagagcg 74940

tttgaacaga atgtctgcct cggacatgtg ttcaacctct tcttcgattt tagctcgacg 75000

tttttcagcc tcatagcgaa tatcgaactc agtagccacc agagatttga tgaatttagg 75060

agaatacaga tgatcctgct tctcaacaag aatgatatcc ttgatggcat ctttcagatc 75120

tttaatctga agctggagtt ccaccagttc ttcgattgtt ttgcgaaggc gatcacgttc 75180

tgcagtgtca gaaggaagag aggtgaacag ttcaacttta gatttagcca tagtaagagt 75240

ctccttatgt tgttttaaat taacgttcta caaaaatacc gttcagttca ataaccagga 75300

atttctggtc ttcactatcg tctcgtgcca gcacctcaca gacgtatcgg tcatccccat 75360

catcatcgca gtcaaataca agactgagga ctttatcatg gtaaaccttc gccactactg 75420

cctcgtaagt attgccgatt gtgaatccaa gataatctcc gatacatttg acaacaacat 75480

ggtcgccagc atttaagtca atcttcgtca ttactgtgct ccttgggtga taatatggta 75540

tgtaaaactt taaccagaga tgtgaataac gccccaaaac acaccgacag cgctaaaaca 75600

gataaggcca tcattaaagc ttcagtaaaa tcagtcattc ctcacctctt gttagtaact 75660

atactatcat ttatcttcgc cgtcaagaac ttttttaaat ctctcaggaa tatctcctgt 75720

tcttagatag ctcatgacct cccaaggttt ccacttgaac ttcttgcaca gtaaggcagc 75780

tgagatcgtc tcgttgcgaa aagaaaggat attaatatcc cactctgaga accctaattt 75840

ccgaagagtg gctatagaaa tcctcacagc aatccaacaa gagaggcacg caacaaacgc 75900

cactgccaca acccacatca ggatgttcat acagccccct taatgaaata caagacatag 75960

tgttaaggtc actatccagc aaataattaa cagtgccgtt gcaaccttgg caagatttat 76020

catagtgtct ctaaaatttc atacccttcg ggtcggaaaa caagccactc aggtgctcca 76080

tttggtcgat acgctttata cagaacaacc tgatctcggc acatgcacat ggctacatag 76140

ttttcatcca cacctgcagc ctgcattgca tcttcttctc cggcaacagc cttgcttaca 76200

acttccttgg taggcaggtg ggcgatcagc tcgtcaccgt cgttaattcc aaaatggctg 76260

agttctccat tggtatcttt aatcttgatc ttaaccaaat ctttcattag tcttcctcca 76320

ggttcaaagg tcctaagatt tcatatgcca gattagaagt taccagaggg tcttctgcat 76380

ctttagtatt ataccatccg tggataacca gagtcccact tttcttcatg tctaactgga 76440

actgttctgc cgtttcctca tccatctcca agatctccat gaggtatttg atgttaggaa 76500

ggtatgcagc tgttacagtc ccctctacca gaccttcagg ccaacctaca tccggatgac 76560

aggtaggaac cagagaggtc agtttaatag tgtatgcttt ttctgcacgg gctttcagta 76620

acggatcaat tttcattatt ctgcctcctc ttcggtgcct gcttccagta gtaagttaag 76680

aacatcttca gcagaaggtt cgtagaactc ttggccttcg ttgtcgatga tacgatagtc 76740

atcaccacga ataatcagag gatcgttatc aaaaccttca agacctttaa caacgacaac 76800

acggtcgatt gccatcattg taagaacttt atcgacatct tcctctgcca caccatactg 76860

cagcaggatg tctttggtgg gaggaacagc atcaaccaca acaccgttac taaaaccttc 76920

cggaagttta gctaaaatat cttccggaac cgcattcata tcaaaacctg tgatctctac 76980

tttcatttac agcacctctt cgtcaactac ttcataaaaa tggctcggga ttgcaatagc 77040

gttctcacct tcacctaaga acactgtccc actaacgatt aacattagaa gaacgtctgc 77100

gataccttcc tctgggacgt tgccaaaacc tttaatcagc tcgtaggttg ggagatttac 77160

ctctaagatg tcaccatctt tagaacggaa atcaacacca tcctgaatct ctgccgtttc 77220

aaggtttttc aatttaatct tcatcaatca gtctcctgtc ttgttaagat gaaggtatta 77280

ttgcacacag ggttggtgtt gtcaacacct gtgtggaata aaattactga aacagtgcag 77340

ataaattacc ttctggtggg aacttatctg ccaagaacgg agtcagaaca gtggccttct 77400

gaagatctgg agtccactcg ttgttcagaa catcccagta gtacgcaatg ttgctgtgtg 77460

ggtgccgcat tacgaacttt ttcccttcgt agtcagttga aacctctaca gaagaaataa 77520

caacagaatc ttcaagagtt tcttcataga aagatccacc ctcgaatttc caacgagatg 77580

caagagctcc tgccttaaac gctgcagata acggactgtc ggcaagaaca tacatttctt 77640

gcactctgtc gctgcgatct ttgcttctta cgttgtatac agtatctgag ctatactcaa 77700

cacggtatag acttaccatt accgcacctc ctcaataact tccacatctg aacccaccac 77760

aaatgcaaaa gggcaggttt catgaaagac agccggatca ccgccaagct tgctaactat 77820

ttctctacca ctaataaaca caagctcacc gatattcatc agattgctga aatagtatcc 77880

ataatgatta aagaatgcag cagggtcagg cagactggta gcctcaagct ctacatcggt 77940

tgagattact ttcttatcat accctctggc cttttgagat gcaagatctg cagaatcctc 78000

aggcgaccgg agatgcacag gaattgtttc accaataaag cgtactttca tattctcacc 78060

caacaaccat agcttcaaaa ggggtcataa acgaataaac agcattatca tctgcttcag 78120

gaaatgcccc caaagacttc atgtctgagc ccttgacaaa tacaaggttc tgatcaaaag 78180

cttcttgtgg atcaatccga tacatatttc gtagggtatc acttacctca tcatcaagag 78240

gcgagtatgc tgtgcacgtt tttccaatca agctttgaga atcagggtat ccacctgaag 78300

ttttaaaata aacaatcata cagtatcacc tctggtaaga gatggggatt cctttcccac 78360

aaataattac aaacctttct tgtcgaggaa gtatggctaa cccaagatta ctcctctaca 78420

acctcataca catccggagt tacaatcaac tcttctccgt tacctggagc aacaaacact 78480

accaaacctt ccggtgtgtg ctttttagcc atctcgatga catcttctcg cagagctggc 78540

tccatcagat caaaaccttc acccagagca tcacgaacca ccttcatgta gtccactggt 78600

tgtgcttcaa cgatcatgcc ttcaaccagt ccgcagtttt tgtctgccag ctcagttttg 78660

aataatttaa ttttcattta gaggtctcct caattatttg atattaatag ccgtaaccta 78720

acacattaac tacaactgcg attgcaaaca taatttcaaa gcatgcaatc tctacatttg 78780

ggactaattt cttaatcata cccattgtag cacctcaatc ccaaaagtca atctttactt 78840

taccttttcc cttacctttc tttccaccgc atttagccaa ctggcgtgct gcaacatcca 78900

ccatacggtc agttgcctcg taagcatcga tacctttagc tttggcgata acttctttcc 78960

cagaattgtc aaaaataaaa gccataactt cctggtgatc attttcaact tttacactca 79020

cacgtgcata gttggcagat gggtgcaaac gctccagttt ggacattttg tgggaaatga 79080

aagacaccat accttcagtg atctctacgt tgccagtgat ttcaatctgc ataacgtgct 79140

ccttttaaag aagagggaac caaaccctct ttgaaaataa tgatactaaa gatcaaatgt 79200

tgtgtcaaca gtttttacag cttgtcgtcg atagttttta aaatatccac tgcaagatct 79260

gccattttac gaacatactc tgcaaactca acaggatcct tgatcaatga gttcacaggg 79320

tcatacccta aagcttttaa aatggctaca gcatccacga ccttctcatc atttgcgaag 79380

atctcaccgt caaacttgta ggtccatttt gagtcaatct tggcatggca ggattggtcc 79440

atgcttatta caccatcttt tttaaacgat tcggtgttat cagtatccaa aactaaatcc 79500

atcatgtaag taccccacta tttcagctta tcttttcttc aacaaccccg tagctcgggg 79560

cagagccaag agctttctgg tttaaaagtg gtgtagcaga atctgcaaac ggtgtccagg 79620

tttttgttct ggcattccag taatgcttca ctctggaatc aggatgtact ataacaaaaa 79680

ctttttcact tgtcaacaca ttaatttctt ccacaccctt actgacaatt tctccgtcat 79740

agcccccagt ctggtaccct tcgtaccaag ttcttgcgta ggcatagcca gcctgccagc 79800

atgcagcctc ttcagaggta gagactacgt agaagtcttt ctcaaattcc gggatacgcc 79860

cttgctttac gctccaagct ccgcgaattt tcattgtaac tttgaacagt ttatttttca 79920

caacagtccc caatcacggc tctaaaatat ctgcatctga ccctacccca aaggcataat 79980

acgagtcatc tccgaaacaa tattccgggg caccagccgc gatcaggtct gatcccaaaa 80040

tgaacaccac attgtggcct acatggctgt gattttgccg cacaggatca tcccacggca 80100

acccttcgaa gtatacagca ggtacgacct ttccattcag gaatgattgt cccaagtaga 80160

cgttagagtc aagaatttta accttcataa tcgtcctcca ctacctccac atcctcccct 80220

agggcaaagt tgtaggtcca ctgactgtca aagcattcct gagacgcgcc agcggcaata 80280

agatcttccc cttttatttt taaaaggtca ggataatctt cgttagggat agcggtaaac 80340

acttttccaa ctaaaggctc ttgaccaata tagaagtttg aatctaaaac tttaactttc 80400

atcagcggtc tccatcggga cgtaaacact gttacgaatc tcgtcagcag tgtagtaaca 80460

agttttcccc tctctgtctc tggcagcaat gtattcagct ataccaataa gtgccataga 80520

cttggtagcg tatctcttaa gctcgttagc tccccagcaa aatctctgcc aaaacggtat 80580

gaaaccgaat aggtacactt tctgttttac tatccaataa gtgcctttac agatcacttt 80640

aaactctgca gcattgctgc taacaccatt gctcattatt ttctcctttt atttttctga 80700

atttggcaag tattgcacgt tggcaggagg aacaccggat agctcccaaa caaccagagt 80760

gctgtaccct ggcacacttc taacatttaa aagggtagga gcaccaacac atttaaactt 80820

ccttttccca aatccgttct ccatcataac ccagtatccg gtatcctgat cacctggaat 80880

ttgtgttcca tcattgtcca cccagcggac ggtccatttc gatacagcaa cggcccggta 80940

tttaaacttt ttaaaccact cgaacagttt cataagtcag cctcgcatag acataatcac 81000

tataaacagg caaataatga atccgactgc cacgccaccc ataaacgtta atattgatgc 81060

aatcataatc aatcatcctc gcttggcagc ccttccattt tgacataccg gaaagtgttg 81120

ccgtaatagt tgatccatga catcagctca ctttcttgct cttcctctgg gattgactct 81180

cggatgctga caagttttaa gaaaacccaa tcacactcta cacccatcct accaaacagg 81240

caatagtgct tcataaaacc ctcccgcctg gtttagaata ttgcttcttc cattgcacct 81300

gagagcaaca aagaggtaat atggctaaca ttcatctcag actctacttc ctcccctttg 81360

aaaggttcgc ggcagaatag gactgtttca ggacttttac ttcgaataat ataatacaaa 81420

tcattcaaag tgttgtagta ataagtgtta aaacgtaagt ttttcatttt ctagtcccct 81480

tcttagattt ttgtgcagaa acgctttctg caatacgaga caggcgttct tctgaagctt 81540

tacctacccc gggtttcttg agcgggttat ctttatgacc gcaatccaat tggatgctag 81600

atacctcccc aaccttgtaa gtcaattcta acaaaaggtt gtcgtccttg tcaaatgttt 81660

ttacgataaa ttttttatcg tcctcctgaa cttcatacct agtacctgca gtatacagca 81720

tggtgccatc agtgtaggat atccaaatca ttgcacctcc ttctgctgta acagcattaa 81780

ctctttgttg gaaatcttta ctgctgtctt tattgttcca tcttcacgga accacgacca 81840

gaattctaca gtgtcgccac tatcaagcaa ggcatgacca tggtaaaaat ccttgtcctt 81900

agacagtttc tcatctatat ctgcatcgac acattcgtga tccatagcat tttgcctgaa 81960

tgcatcggtt atgccaatgc atgccttgct ctcaagactg gtatatccga tatcttttaa 82020

ttcagtttcc caagtccagt agcctacaaa agccacaaca gctgcccaca tcaatatcga 82080

cttaatcttt ggcattctct ctacctcttt cttagtttat ttaattatgg cacatccttt 82140

gtgccttgtt gcacactact tgaacaacag ttcaagatag tgcaaaatta acagaagttg 82200

aattacacct agcacaacgg cgagtatgtt aatcttagaa tcaaaaaact tcttaacttt 82260

taccctaaaa ggctccttaa tgtcctttac gatcctattt atagatgatt tcatatccct 82320

gattaccatc ctaaacatta gagacggatc ttccttctct gtaacagaat cgaaaacata 82380

ctctcttcgg tcataaggac ctccagatct gcgtattaac ctctccacag agacctcgta 82440

cgacacacct gtaacgtaat catctatgat aagtagtgac gggtctttat cgtccaaggg 82500

tatcaaagtt atctttccag attttgttaa aatttctcct ttttcaagca aggtgtacca 82560

atgctctctg ccaaggttgt tgaggcacgt ttttacatat ttaacaaaca tcgtcacccc 82620

tcatcgcacg tctgatatag ccattctata aaatcttgat accactctcc ctctgagcgc 82680

tccgtaggcc aataatcttc cccctctctt gcattaaaat ctgagtattc aactttccac 82740

tcctttacca gctcgtggag aaggttttca gcctgttcta aggatttatt ttttgtcatt 82800

actgtcccct ttttcaagat aattatacat cagaactgca ccacctaaga ttgctaccag 82860

caacaatacc ccagatagca tctcaataaa catggcttga aacactagaa ggtcccctct 82920

aatttcacgc accattttaa atacatattc ttttcccata aaggtgcccg gctaagatct 82980

gcgtagaacg caatctcttt aagaacagta taaacagttt taggtgcggt atccatccct 83040

gctctccttt ttctccttac gggtattctt gtagtaatca actaggcaca ttattactat 83100

gaagataaca acaggccaaa aaggtgcagc agtgatgatg gtgataaaat catcaaactt 83160

cacccttcgt gtagacacga tgcatatacc tgtcaagatt actgctgtta taacagctac 83220

tactaggtaa cctattagca cagcatttat actcattggc ccctccttcg tatgttaaat 83280

tgtgcacttc aggtggttgt cgatggtgtc aagaaaatta tcccgttctt tctcagagtt 83340

ttcacggatg tagctgaaaa acatatctag aataacctta ttgatatatt ccaactcctc 83400

tgtgttcagg tacttagcag gatctacaat gacatcccaa gtatcataca gaccgcactc 83460

atgatacccc caacgccgct caattgtcaa actaatcggc aaatctttac cgtcacaaac 83520

aaccctccat ctacagatac tggtatctgg gcgtacctgt gcacggtata cctgtacaaa 83580

atccaatttg aatttgaggt actcgtcata caatttaagc acaaagcttt cgatttgtgg 83640

agacactttt gcagcaaact gtgcgtattt gtacttgcac tggatgatct ttctgcgttt 83700

tgcagttaaa ttttttgcag ccagttgttc ttcagtgtac ctccacctac gatcaacaaa 83760

cgcaacaggg tctttgtcac catagaggtc ttcccagtta atcagaagca tacctgtcac 83820

tcctccacaa cttcaaggcc tttgccgcct cgttctggta gattacttaa agacaggtaa 83880

tacgtatagt caggagagat cctaccagac tctgcacctg cagatagtaa atcttttccc 83940

ttaaccatga caacattatc gtttgcagat agtcgttttg cttccacaat tttaccaacg 84000

cagtcaacca aggaagggta ccctaaatct gtaagaaatt taactttcat gctacctcct 84060

acttctaccg tccctggtaa gataaattat tactgaacaa agcgcggagc tgggaaggat 84120

aactcttcta aagagaagat ttcattcccc acaatccatg ttgccggatc atctgggttg 84180

actggataga taatcatcac ctcgtccggg ttacataccc gctttttacc tgtggagtta 84240

tcagcaacca gcatgcgcgg ttcttcagaa atgcacaggt agcctcctcg gcccactgtt 84300

ccttccacag ggtcttgtcc tttgatcacg atgattatct tctctcctga ggcgatatta 84360

ccttgcacac tcactgtttg accaaccttg gatgtgccaa tcttagtgac ttcgaacatt 84420

acatcccgaa ttaccgttgc ataatcgcca gacactttct taccttcttt agtgatctca 84480

atcttcatac actgggctcc ttaaatgaat ctatgtaatc aattaaatcc caagcatccc 84540

tatgacctgt cagagggtat acttctccaa gagggatcat agcaataagt ctatcaaaaa 84600

cccaatctgg acgcaacatt ttatggtgat actcccactc accttctaca gactttttct 84660

gagtgatcag gatctctgtg tagtgtttcg cctgtcttga gtagattgag aatgacttgt 84720

acataaatga ccctcctctt gttgaaaaca aaatacacta ctaccaggtc actgtcaagt 84780

aggcaactgt gttatttttc tcaggagaga tgccaacaac atccacctgc agaccccctg 84840

ccctcaaatc cccaacaatg tgtgggaagt agtctgagag gtgtgatggg agatttatcg 84900

aaaatccaca tcccagaaag cctgctaatg ctttattttg tatctcccct acaatcgcat 84960

cataaataga gttaagctcg tcaggaagcg ttacaggacg ttttagcgct aactggtgca 85020

acttgtcctt agcactcatt ttcatttcaa gtctgcctct ggtggtgcca accacataaa 85080

tggcggcaag cctagacggt ttttcttgtc ccatacaaaa taggcatatg cctgcccatc 85140

cgttctacca ttggattgga atgacggacg cttagatatt gtgatcacag tggtaggtgc 85200

attatcaagc caccattcaa atctggcctt gctttccaga aaattaagac gcaaaagcat 85260

aataactacg tcggcatctt tcaacgcctt gtccacaaac tcacgagcca ggctgtacgg 85320

cgggttggtg ataatacagt caacatggtt gtattctgta ttcagatagt caacaccttc 85380

ttggatttct ccccatgcag atcctaacgg catacgggag tagaacacac ctgaggcgct 85440

attcctgcaa ggctctaggt aggtccagtc ttctgggaac ggaataaggt catacagccc 85500

ttctgcacac cactccggtg tcacatactc gtcgtaagcg ttacgttgtt catcaggttt 85560

tctagcacaa gtcatgcaaa tctccagatc ataaaagccc tccgaagagg gctttagaat 85620

taatgtttaa gcttcttcga caattcctgc acccaggatt ttttcattga gctcttttgc 85680

tgttttaata atattgtcca ccagacgctt gcgggtctta tctttaacct gaacttcttc 85740

acctccgttc agggtggttt tcaattggga caaaatgtcc tgaagctcta ccacagagaa 85800

tcgctgcaga tcttccgagg taatctcacc cagtgcaatc ttaacggtct caccactctc 85860

catctgcaga ttcaggaaca cagtgtgaac atcacctttc ataccattta cttttgcaga 85920

ctcaacagca tgaaccagct cgtccagaga tgtgcattgg tgaatctctg gcaggcctgc 85980

ttcacctagc acaccttctt catcactttt tgccaaaagc tttttcggtt tctgtccatt 86040

ctgaatgcgc tgaatgtttt catcgtccag aatgatagca ccagttcgag atgccagctc 86100

ttctccatct aaccacactt cgccgccagc tgtgatggat gcgatctcat cttctgttag 86160

cactccaccg tagtattttt cgatcaggcg ttcaaaacgt ttcttccagt agttgacctg 86220

ggtattcatg ttcacgccgt taccataagt gccaccttga taatagtgga acataaattc 86280

tgtgtccgga gccacagttc gttctttgca acccagccat aagattgttc ctgctgagca 86340

gcacatccct tcagcgtgta caacaacgct tgcctgagac tcacggaatg cctgcaaata 86400

cgccatagcg atgtgaacgc aaccaccagg actgttaatg attacacgga tactttcttc 86460

tggctgagcc atacggatcg tctgtagacg agacatatga tcttgcaggt cttccaaatc 86520

gtcgatataa agaacgtggt caacaacagg tgttgggtag ctgtatgttt ccataatacc 86580

gcccggcata ccaaacattt tattactcat aggttcgaaa ccgttctgtt tttctttcat 86640

gccgtgtacc atggtgctgc gaggtgttaa attcatgaga aatccttaat attggaagtt 86700

gaaagctatt tatttaaacc tgattcacat ttacctttat ttagcaatca ctatagaata 86760

cttacgattg tcagaagcat cacagctcaa cgattgccag tctaccacat accacgtatc 86820

attgtcaaca attactttat cgcctgttct gtagcaggtg ctgctttcaa taatccgaga 86880

ctcgataaag aatctgtgtc ggcgctcacg tagcaacgct aagttgatta tattaccacc 86940

gctgtccttg gtccacacat tcttggatcc cttatttgcc aaaattgtct cgccgttata 87000

tacaaacagg cagtatattt tattcagtct ctcttgtatt gtagccatac tgtgcccttc 87060

tccttgtttt gttcaataat aatatctaaa gctcacctgt ttgtcaacaa ctgttttaga 87120

tatttaaggg tggaataacc acccttctgt attactcgga aacaggagtc caaatatgat 87180

ttgcacgatc ctcaataaga ttgagtgcaa tctgacggcc caacccggaa cgaacgacat 87240

cgtctggact atcaaagcta atcattccaa cctcaggatc tggatgacga gtaaagaaat 87300

ctgtcaccca tgcaaggcca gatttaccag ggatgtcacg ttggctatta tcacccataa 87360

ttacaagggt ggcctgatca gagatccttg tgatgattga caacatttct tcaggattgg 87420

tttgctgtgc ttcatcaata aggacaaagc accgttcatc aaaactacga ccacgaatgc 87480

tttcaacctc acaaatctcg atacggctgt tctgaccgtc cccaagcatt gctgcaaaag 87540

cacctttgcc aacacgacga caaatcgtgt ccatcatgct tctaagatag ggccacagtt 87600

tctgcattgc atttccaggt ttaaatcctg aagtctttcc tgtctgaacg taagggcgtg 87660

caacaatgat cttatcaatc tcgttcttgc gcagaagatc acctgcatat gcagatgcca 87720

tgaatgtctt accggatcca aacacaccgt ttacaatgat aatcttacgc gaccctagat 87780

aatcaagata cttttgctgc tttttattca ttggtcggag agctggagcg gtgtgctctc 87840

gttcgtcagc aaatttttca gcagccttac gcaggcgatc agatttgcga gcctctctgg 87900

cttcatcacg catgcgtttt gtttctttag ctcgacccat gttgggactc ctttcagatt 87960

gagatgtgcc aagtgctttt actgcaatag tttttcaata gccgcttcca tgctgcttgc 88020

aacagcctga actcggatat tcatctcttc ttggtggatc agctccttaa gctctttaat 88080

ggtaaattct ttagagaaac cttccaccat aatcttgtgt aaggcttctt gcgtatctac 88140

ctgcaagaac ttttccagtt tgttggcaga ggcttctaaa tcagcccgaa tacgagggtc 88200

atctgtgcga ttcagcatca ggcgtaagcc atcaagatta gtagaaatag cgtctgcatg 88260

ttgagtaact aaccgtggca aaatgagatc tacaaactta ttagccaaat cgatacgttc 88320

ttgtgtcact gttgcacccc ctcttcatcc tcatgatgtt tcttttcacg cttgtgtttt 88380

ttcttaagct caagcgcaat ctcttttttg tacgccttct tcaaggcctt tttagccatt 88440

tggcacctcc tctgctttca ttacgctaat tataaccaac ttaacaagac tttgtcaaca 88500

attagtcaac ctgaacccaa ccgcctgcta cctttttaaa cattctttgg ttatcgctgc 88560

ctcggaatgg tttcttggtt ggtaaatctt ttacatactt tccatcaatg acgatatcag 88620

ccagtttaag aacatcacta gagatatgct caaatgtgaa cccagtatac acaagaatag 88680

ttttcttgtc accaaacttt tccttaattc tcctcataag gtctataata cctggctcat 88740

tataagtagc caaaggctct ccaccaagta aagaaatacc gcatacagat tcattgtcta 88800

aatccgacaa aattttctgc aaatcttctt cggtgaactc tcttccttta tcttttgacc 88860

aaaatgcttg attaaagcaa ccaggacaag cgtgtgggca cccagatacc cacaaagctg 88920

tcctaatacc tggtccctcg gtgtaagata tctctgtata cccgacaata ttcatacata 88980

ctccgcatcg catttaggac aatgccaaat aaaatcgtcc agtgccttac accaacgaag 89040

attgcaatga caaccacact tgcaaatttt ataaatacct gtagacatac tgcacctcct 89100

agtttactgt gtgacaacaa tatgccacat ccttgtgact tttgtcaagt atcagcaacc 89160

acaacttccg tagtgcttaa cccgttctat aacctccgct tgcttacctt tattgtaagg 89220

acgagagttt ggactggaca gatacccgga tacgcgtctg atcacactga tcgttccttc 89280

ttcatggttt ccacattctg ggcatacaaa gcctttagct gttgcactga actcaccaga 89340

gaatccacac ttaagacatt tatccacagg ctggttaata ccaaaataca taatgtgttt 89400

atatccgaaa tcaacaagag cttccaaagc atctaagttg tttttcagat ttggtgtctc 89460

tatgtaccca atgttgccac cagaggagat catagcaaaa ccttcctcat acttccactt 89520

atcaaaaggc gagctattga tccatactgg ctgatggaaa ctgttggtga ggtattcatg 89580

ctcgtgcttc aacacttctg ggtattcacg atccagacag gttgcagctt tatagcataa 89640

actctcagaa ggtgtgccgt acaagctaaa tgctagtcca ctttccttct tgtatcgctc 89700

acaagcatct ttcatgtgct gaaggattcc aagagctaaa gccttgtcac cctctctgcc 89760

caaaatctga caggtttcat acacaccaat atacccaata ctgatggaag cgtacccgtc 89820

atagaagagt ttatcgatac tctcttcagg atccaggcga gcaagagctc cttcacacca 89880

catgataggg ttctgccctg cctttgttcc ttttagacgg ttaaccctta caaggtgagc 89940

ctcatatgcc aaatccaaat attcgtcaag aactgcaaag aaatcaacac cttccgtttt 90000

agcttttgcc gcaatcattg gtaggttaag gctaacaaca cccaagttga atctgccaag 90060

gtatttctcc tcgcctgtta cagggtcgct atatttcgat aagaaacttc tacacattat 90120

gttcaaggtg gttcgcaagg ccacccccgc acctttacgt gctgctgcat gtccccatgc 90180

agatcagact atatcttcac acttagtgtg tctaccattt cgagtgtcat cagcttacac 90240

cctacaccgc tacattcatc acggttagtc gttaggcatt tcgtaacaga actcgtaatc 90300

atagttatta ggtgcaccac gtttcaggat agcagatatt ctttttctat ctattcctaa 90360

aacatcagca caacatctca gactcttaaa gtgaataacc acaccagtta atttgtgggt 90420

ggctttcacc ccgtgacatc ttgtgtaaaa agcataacag ttgttgtcgt atccttcttg 90480

agtattgtct gggcaagctc cccactcaag gttgtatacg ctgttatctt tcctgttgtc 90540

acctttgtgc ttaacatatg gtaaattgtc tggatttggt aaaaacgctt cagcaacaag 90600

tctgtgaacc cttaggcaaa cagtcttttt gttcttgcgt atcctgacaa gacggtaacc 90660

tgtgtttatc tcttcccacg aatgaataat tctttcattt tcgtatcggg tgcaataaac 90720

attgcccatc tcgtcaattt cataaccttc aaaatcacga atttgttttc tcatttcagg 90780

actcctctta gtcaggagtt ctgttacgtt tagcacggga ttgtccgttc tggagtttcc 90840

ccgtttagat agattttaca tgagcagtta tagtttaccc atgcttgtta ctttgccgtc 90900

tgttgaacct gtcactttac gattcagcgg aacagaaaca aagtcaggat aaatccgctc 90960

tgcagaacat tctagagcca atcgtttcaa atcgtagtta ggatccccag ggttcatgtt 91020

gacaccctcc tctaagaaga acagcacttt agggaacaca ggtgtgatcc gttcttcacc 91080

aagacccatt ttgtgaactt tcaagtagtt tttggtaatc attctaccaa agaccgaggt 91140

atctagccct aaacttaatg tgatgaatgg agtttgcccg ttgacagaag ttaacgtatt 91200

gatttggtag agcaaagttt gcatactatc aaacacgtct ttatctgtca tttgcattgc 91260

aacagtctca gcatctggca accaatgctc ctgacaaaat ttaaggttct tctcataggt 91320

tgcttcagca tactgtgcca gataatgatc aatatggctc atggtttgac caccatattg 91380

agcactagct actgcactag agatttggct aaggattgtt gttgcaaccc ctattgactt 91440

cggcttactg acatgggcgt tgccaatgtt gaatccgttt tccagcatat ctggataatt 91500

taccaaacaa cagtttgtta acggactgat caagtagtca agatcatgca catggatgaa 91560

gcctttgttg tgctcttcgg agacacggtc tggcaggatc tgtgttgatg caagatgttt 91620

gctaaggatg cctgcaagca ggtcacgatg agtatgtaca tgattagtag gcttatttgc 91680

gttttctttt gtaaattcat cacaagtacg atccaagaag ccgctaatat ctgcaagcag 91740

ttttccacct gcctctctgt tattatctct tgtctgtcta tactggatgt attctctggc 91800

tgcacatttt aaacccatat ccataagagc atcttcaaca aggatatgga tattttttac 91860

agagatcgtg cactggtcgg ttggcagatt gttggcaaca acttcattaa cagcgcaggc 91920

aatccggtaa gcttcttctt cagtatgtcc agactcaagt aaggcggact ctgccgcctt 91980

gatcacctta attccgttat aaaactcttc tctaccgtcc cgttttacaa ctttcacttt 92040

ttctctccaa aaccattctt aacaaaagaa atgaaatcgt cagcaccacc tatgtggata 92100

ctgtcaaggt tgctttccaa gaagatctga ggcaacgttc tgggaacaaa tgggctacac 92160

ttctcgataa gctcttcttt tgtgtatcct tcacctaacg tcttgtatac gaaaggtatg 92220

cctttgctgg ctgcaaattc ttttgctctt acacaatgtg ggcagttatc cttgccgtaa 92280

atgatgatga aaaaagattt ttcgcagctc atatattaaa actcccaaat ttcttcttca 92340

gcgtcgttaa cggttgtgtt gacgcggtag tcggtgttag acatttcctg gttagccgtc 92400

tgttgcagtg aaggtttcat ccacacctgc ataaacggta gcgggtcttt tgttggcggc 92460

acaaagtctt tgtcgatgtc gtaataatca taaatcggtg agcagttata gtcaacccat 92520

tctttaagca acggtgaatt aagacctata atcgctcttc cttcgctgaa aatatagtca 92580

ctccaaccgt actccttggt ccgcacttcg tcaagaattt cttttaactc acccttaatc 92640

tcaaggaagc tttgctccca tgcaggatca tcaagcagga tatcgatgat agcaaaatca 92700

cctttcgaat gcagcatttc atcaagcata attttttgaa caagttgtgc cacttgaaca 92760

aaaatacctt gctcagctac tgcgaacgta catgcaaaac ttgcaataaa ctcaatactt 92820

tctagggcta aaagtgcaaa aagtgctcta aggatacatt tccgtacaac atcttcttca 92880

acactggaag gatccagtcg gtattttgca ccaataacct caagctcttg catatatttg 92940

acaataactc cagacctgct cagcacgttc tggttttcct gaatctcttc tataatctca 93000

cgagcattag gaaggcattg tctaacaatc tctgagtaag ttagtgcatg aagaacctca 93060

atctcagact gcttcatcaa caaggtgaag agctcactgt tggtgataaa tggtgaaaac 93120

agacaaatga tagatcttgc tgcaacactg tctgcctccc attgccacat cagtgtctgc 93180

accatcacat cgtaagttga ctttgagcat gttacgaaat cagaaataga ctgtgacaaa 93240

tctacttcgt cctctgccca atcttgttct ttttgttttt tgtatagttc aaacagctgt 93300

ggatactttt tgttaataga gtcgtacatc cccatctgct gtcctagaaa cagcggataa 93360

tcaccagttt tatgagcagt gttctcagta ttaaatacgg acattattct acctctaaaa 93420

tgggggccga agcccctttt aaattacagt gcacaacttt cgcaatacgg atcttcaact 93480

gaactatctg caatcaaagg atagttcttt ctggtttcat cttcagcctg tttcttcgcg 93540

tgaagctcct ctgtcaaaga gtcaccagca ccaacccgag agtttaagta ataccatgtc 93600

ttcatcccga atttagtagc acggataaga tacccaagct gctccttcat tgaaatttta 93660

ccatctttca acttggtgta atcaatatat aggtcagcac tgattgcctg tcctgtgaat 93720

ttttgaatca cagcatacat atcaatcatg tcgttagtat caacgtccca agccgaggta 93780

taatactctt tcagttcatc atattcagga acgataaaca gcacctgtcc ttgtggcgac 93840

tttttaaaga taatgtgctc acggacagga taaaccccgt tagttgtgtt tgttgccaac 93900

gaggagcttt cattaggcat atatgcttca agaacgctat tacgtagtcc accgttttca 93960

ataatttctt tacgtaaccc ttcccaatcc tgcttaagag aaggatcttt caccacacta 94020

tcgacctctt tgttataagt gtcgattggt aaccaaccct ctgggtattt tgttttatgc 94080

atccactcgc agacaccacg ctcttttgcc aatcgtaaac ttgccttatg caagctgtag 94140

ctgtgaagct ctgcaagatc gtgaacaagc tgtttacttt caggagaccc gtaagcaacc 94200

ttgtggctgg caataaaatg tgcaatattg gtaaggccaa ccccgatgga tctgcgtttt 94260

ttaacagtgc tctccatatt ttcatacgga tatgtcatga tttccataac attgtcaatc 94320

ataagcacag tgtagtatgc aacatcctca tactcctctg gggtaaccct tccagctaca 94380

agggatgata ggaagcacaa tgcaacctca ccatcatcag catcgtactt gaagatatta 94440

cgcatatctg aatagccttt tgtcggaagg gctatctctg cacacagatt acttgaatag 94500

attacatctt taaaaggtgt atgcctattc atctcatctg tccagtacac atacagcctg 94560

cctacctcgg tacgctgcgt gatcagttca acagcaaggt ctctagcacg gatccatgtt 94620

ttaggaatgc tgtcatcttt agcgatacga tcaacctctg catcaaactc ctccatagac 94680

atggtgaaga taccctcgtg cagttgaggt gcgtgcttat aagaaacaag catccaatct 94740

aaattcttag ccacacggtt gataaatgat ctattaatac ctatagagta gtccatagtg 94800

ttgattcttt ttgaagcaac agtcataggg tttctcaaac ggatcagttc atcaatctgt 94860

ggatctagcg caaggaatga taccgttgcc gaacctccac gtgattgttg tctattctcc 94920

ttaaccccag catcaatacc tcgataatat ggaagcttgc ccatatgttt aatagtgtta 94980

ccacgaacac catcacctgc agatcttgat gatagatagt agccaatacc tgcttggtta 95040

accgtcattg tgtacgcaac ttcacgtgcc acaccaatag attttgctgt atcgtcagct 95100

tttattacgc aacaagatgc ataccctgtc ttacctgtgc gtagaccgtt taagtacggt 95160

gtcggggcat taatcttcaa gtccgacaga taagtgtata attttacaac atcgttgata 95220

cgacgttctt tcggcatatt cttcatgaca gccattgcta tccccataaa catcatctgg 95280

ggagattcaa acagggtgtc tttaagagta tccttgatcc cgtatttgtc gcgaaactgt 95340

ttaaggactg catagccata accgagatct ttactgtgat caataacagc gtctaaagtg 95400

tttaactcgg taaagctata acccatatcc tcccaatacc ctttatcaac catggtattg 95460

taaaatgagg gtaaggaagg gatctttgta aatcctccaa aagcctcttt ataaatctga 95520

ccgataagaa cacgaccagc catacgagaa tgagcttcgt ctttacggct aacacaggag 95580

tctatgatag cttggttaac ttctttggtt gtacacccat catacagccg tttgtatgca 95640

tcaagtgcaa tatgtgacca attgccgtta ccgtcgtcac cgaaaatagc tgccttattt 95700

aaacgttctg gatcgaatgc cactccacat ccgttgcttt taattacctt ggtgatcaat 95760

ttgcttcctc cacttcatca acgtatttat caaggcaggt atcagtgtct tgttggataa 95820

aagtatcctc ttctacagag atatttataa ttttacctgt atattcatca taataaacat 95880

catcatacat tttaatatcg tttgtcagac cgttgaacca aagccaacca atatactctg 95940

cccgacttgc aaaaccaaga tccttccact tggagttatg actcatcttc accaccttag 96000

ctttcaatac cgaataattt ttccaggcga tgcagatctt caaaattgaa aggttcatca 96060

acaatatgaa tatcttcagc cttttcagat tgttttgtat actttctcac attaacacga 96120

tcttctacgg ggatatcata cggaagatct tgaacaagca ttgcctcacc gggtgcttta 96180

tctacatcta aaatatctac accgttatac atctgcgggt atctaaagtc tgcacgcatg 96240

acaccagcaa atacatctgc ctttcgataa tgggttggct tatttttgca tctaacgttt 96300

tttccacgaa gaacatcaat attttttaaa tcaaacccta cagcaaagag tgcagctcgg 96360

agaccgtttc gtgtgcgaac gttaaacccg ggaacagtag ataggaaatc taaagtaact 96420

gaaataaatg tctttttagg aatcataaca ttactcatat ctgtctccat ttctggctca 96480

ccaacaaagt gagccattat tatagattat ctaatactat tagtcaacac aaaagttacc 96540

tatcgctgcg ataggattaa cctttcatga agtggttatt aagcagctgt ctgtgctgaa 96600

cccacccaca aaagttggca gaccagaatt taccgtcgcg gtcttgatgg gttacacctt 96660

cctgccaaac acagctgtcc tgtgtgattt cacatgaaga caggtagtag tttaaacgtt 96720

ccattgcgtt atcgtgatcc ataggtgttg cctggtgctc gtaagggctt gcatgaatac 96780

gatccccacc aatcaatttt ttagcaacac tgtctgcctt ttcaaacgtg ctgtcaatat 96840

ttcgataaga tacttgtgca cagcaactag cactaataga cagagcttct ttagtatcga 96900

gaatgcagag ctttccttct gtgtcacgta cagcgtagtt aaactctccg tccaaataaa 96960

catgctctac atatggggtg tgccattgac ccggtttaag gatttcagga gtgctattca 97020

taatagcatc ctgcatgcaa ttagccaaca tctgaatcgt aggatccgca tcgtgccagc 97080

aacgcaggtg gaaaaaatta ttccactcgg tgcttgttac aactgttttc atgcgctgaa 97140

acggctctac taagcggtta catacttgct tatggtaacc agcaagggcg aacttctctg 97200

catgccaagc ggcgtttttt gcagcttctt tccaaaactc ttcaggggtc atctccatgt 97260

cagtgtattc tacacggatc ttttgatcgt gttcccctcc atcttccatt cctcgcttat 97320

ttacaccgaa gcgaactggc attgccggat tctccacagt ttgctgcagc atcttgctga 97380

caggtactgc acgagaagac atagcgtttc ggctgaacat tcgatgtgtc ataagctctg 97440

agtgaataat tcgaggatat tctagctcaa aagtagtgat acgcttacct ttctcggaaa 97500

tgctgtcggc aataatcttt gcagtgcaca gttttagcat tatttagctc tccttgtttt 97560

aattttggaa atttcgtctg ggtttatgtt ggccttaaat gcaaggtatc tgttaaacat 97620

atcggagcac atatcttggg tgatcctaga ggcgtacacc agcttaccgc caagatcata 97680

cacctctagc cagtcaccgt atctgactac aggatcgcca cctttagaac atctgcccat 97740

ttcaaatacc ttctaaaaac ttccctattt tataccagct gttagcggta tagtcaacac 97800

taaccttcaa ctcctcgaat tgttcataag gagtgtcttt taagatttta ataacttcag 97860

ggcgtgaagc gaattggttc aggtattcat gcctgtcgtc aattataaca tctacagcca 97920

cattaccttt gaaatgtgtg gcgtagaagc cgtgaccctg ggtgtgatcg tcaagatcca 97980

taaaatcgct ggtccaacgc tttaagaacc ttgatttaga tgcaaggtga cctttcttgc 98040

atacagagat aaacgcaagc ttgtgaccac gagccaccaa atctttcata acctcaatag 98100

ccccctcttc aggcttaaga aagtcgtaga ggaacgggtc actccagaaa tcaaaaggcc 98160

ctatgatgtt ttctttgggg cgatcaggcc aatactttga cagatcgtaa tacatcagtc 98220

cgttagaagg tttagcgact gtcttgctgg ctggaccata taaacgctct agccactccc 98280

accatgctgt tcctgagtct accagtgtta aatctacatc aattccgatc agcattgttc 98340

tcagccctct ttagcatcaa tatacttgca cacaatctca gatgcgcact cccaaggagc 98400

tccctcgggg ttaacaacat caaactcaat accttttatc ccgttcttct ctaggctaag 98460

gtaagttctg gaatcatttc cccaatcata tcctgcacgg tggatacgga acagtagaag 98520

atcgccacct gtgatggatg cgtactcgcc aaggcatgcc agctcctctt tgaagccacc 98580

atcggagtaa acaacaaccc ctttaccttc agggaaatct ttcttcaagg atgcgactgc 98640

ggccttaccg aaagcatctt caccaaagat aggtttgatc agattttcac tacaatggat 98700

catccattct cgcggagaaa catgcttgcc gtctatcatt aagaacggac acggttcttc 98760

tttgtagcgg cgatcataga gtgcattcca caactggggt gaaataccag ctgccttaat 98820

tgccacactg aatagcattt cctttacctc gcggtgatgc gagtttggaa tggcttccaa 98880

caaaagatct gcgatatggt cttttccaga acttggtgga gcattaagaa caataatatc 98940

cattaaaaac ctccgtggta ttcttcaact gcatcaataa tacttttaca cttatctacc 99000

agttcgttag ctactacggc atcgtcttcc caaacagcct cctcaatctg atcaagcaat 99060

gatcgcatct tattcatcat ttctgggata aagctttctg aaaagctgtc tggaattgtt 99120

aactctggtg tcattgcggt ctccaatcat actgtgcgtt attagaagtt tgtttttaca 99180

cgtccctttt catctacttc gttgttagct aaaagcgtta caaagtctgt aaaacgaaca 99240

tgaagaactc ttttcttatc caggtatcct aaagccacac ctgagaaatt taccatcaaa 99300

ggtgctaaag aagctctagc actgaaatgc tgctgttcca ctcttccaac acccttagta 99360

taagaagggt tgagattgta agttctgaat ccaagttttg ttggggcttt agaattaaaa 99420

acaactttat aacgtttctt actcaactta tgttccagaa taagcatacc cttttcacca 99480

cggataagct gaaaatctgt aaaatgctct tttaagacaa aaggtagtgc cagccccata 99540

gcctgtcccc tttgcacggc tgtttcacca aaagcctttg catcagtaag ctctttagtc 99600

aagtctatac ttttgatcat acctggtctc caaaatgtat tgtagttctt tcttgcctat 99660

actcttcatt ccagcagtct tacctgcttc ttcttgaaaa gaagaatagc attcagcaaa 99720

cactctgtca acaccctcaa ctaaagattt tatgaaatat gttgattctt cagattttgg 99780

gtcatcatct cccaatactt ttccagcatt atatccaaca cgtcttatgc agaagtcgta 99840

gtcatccggt gtgctaaaag aaaatagctt attctctcca atctctacag gatccctatt 99900

gcacaccttt ttctcccaaa tttggaaaac acatggcacg tcataaggtt tgtcgttaag 99960

taaaaacgag ttcttggggc acctttcgct gtagataagg tgcattgaca tgtccagctt 100020

gtttgcaaaa aatatttttt caaatgtccg tggcaagatg aaggctatag ccccgccagg 100080

ctctagcaat gatgcacagt ggttaaagaa cttgcaagct aaagacgaat ttttgccgaa 100140

tggaggatta cctatgaaga catcagcctt agggtacacc tctaagaagt ttgccttaat 100200

acacggtgtt gggccttcaa attcaggatc tggtaccaaa tcgtacgaca gtatctctga 100260

aaatagtttt gaaaaagaac cctttccagc agaaggctca acgtatgcac aattcccata 100320

cctagctttt actttaccaa caagatattc tgcatactca gggtctgtgt aatatttgtc 100380

gttggactct ttcttgctca aggtgcctcc aaaatgaaaa ggggaggcgc aagcctccct 100440

ctacttgtca gattatctga atcagggtgt ttttcatctc ttctcgggta cggatgttgt 100500

tacgatcacg aacaacagat ccacaacctt cgcagcggta ggttttaaat gcactcagtt 100560

gagtatacgc caaagcctct tcctcgtgaa ggtgttctcc accacaacgt gggcaacgaa 100620

cttttgactc ctcatcttgg tagtacaacg acatgttggg gtgctgctta gcaaacggac 100680

gcgccagcaa atacagacct tccagagaca agatgtcccc aacattatac tcccgcatct 100740

gtttaaacgc ctcaagatca ccatgaacac acttgatcca catttcaaat gtgtggtcaa 100800

tcagtttctg tcgggccagt tggaagtatt tacatgcata ctctaacgtg tttgcattca 100860

aacggaatgc ctttttaatc atcttgaggg tgtcaacctc tttgtatggg cttgttggcg 100920

gcaaaccgtg aaatgcaaat cgttggttgg cccacccaca gtcaaaacca gagttgtgcg 100980

ctactgcgat atcgcattta tccagataat accacaaatc ttgaatcagt tcaagatcgt 101040

tgtggatatc ctcgtcccac attttgtaat caggaagagc acagtcaacc acgctaggac 101100

tgtctaacca tttacctgcg aaagttagca tatatggcat aatttcaata ccgcaggttt 101160

gaatgttggt cttataatgc ccaaagaacc agccgtttgt tggagatgtc tctgtatcat 101220

acacccaaac tttggcacct ttgtgttctt caaggaagcg ctccgttgcc tctcctacaa 101280

tctctgacat attctcttgt gcacgtttaa gctgtagtcg gatccagctc tcacgtgact 101340

cacgacctaa aaattcacga gcaatttgtc tattagtgta tccctgttgc ttcagtgtta 101400

aaatttgctc aagagttaac tttgtcatca tcgttaagat tccttagtaa aactgaagaa 101460

tgtcttttaa agcgaagtca cgatattcgc ccttgtctac atcaaatgca tgcatcaacc 101520

actgctcttc tttatgatac tctgtagagc caaaccataa attgggctca gtaacgcagc 101580

gatctgcaac aacacctttc cagttacggt atttcatcct gatcaggact gaatcgcaag 101640

gattgaatac ctttttcatc cccatagaaa tctctctcct cagttttctt tataaactcc 101700

tgcatgtagg cctcaatagc ctcatgcttg gtgtactctg aattaatgac aagatcaaga 101760

tccgattctg cacgtgcaat aatcgtagct ttagagcatc tcctcaagaa ctttgtcata 101820

tcgtccctgt agtttttgtg gagcgggaag tcgctgtagt ctttctccag atagtcagcc 101880

atccggcgca acgcctctga taaactgcaa tctgtgtact cgttacagta tttcatccag 101940

tatttaagca catacccctc gaaggtatta gtgcattggg acaaaacacc cctgcaacga 102000

ccagtcagat gatcatgatc taaacaaggc ttacgaagtg gttcacacaa gatcggatca 102060

agaccatcct gctcttctga caattctttt cgaaagttgg ctatgtcctt agaatctttt 102120

agccacccgc tatgaacggg ggctttgaca gctttctttc ttattttacg ctttgcgata 102180

atccctccag atgcgccctg cctatccaat aagcatctgc gagatcggcc ttaccttgta 102240

ccaaattgat cccatctagg aacccaggtt cagatacttc gcaggcctta accatcaact 102300

ttttatccat tgtaacaggt ttcttcacag tatgctcttt acctgtcttt ttgtcaagct 102360

ttttcttctc ctcgaaagca agttcctcag gaagatgact acgagcaaac tttttggcag 102420

ttgttggtgt cactgtgtgg accttaaaca ggccgtcatt ttcacaatca gctaaggcat 102480

cccaaagcat aacttcaaga ccgtaaaaca acccagccag ttcacgtgtg gcatcaccgc 102540

gtgatccaaa ggaaagccct tccataacta cggtgtcaac gccgtgcgtg tggatataat 102600

atctgacctg tttgcagatc cagtcaatac gctcagcagc tgttgcaaaa gaaaccgtcc 102660

ctggtttctt ctcttttgca gatgagcttt gagttcgtaa cacaacctta tgaacaggct 102720

ttccatcgtc ccatacaacg caagctgtgt gtgtcaacga ctggtctatg gataatagac 102780

gctctctcat gcttcctcat cctcctctcc ttcatcagga gcataagcct tatctttgaa 102840

caagacgaga gagcaaagct taacatctgt ttccacactg gtgaacatgc ataaccgctc 102900

gccctcaatc agctcacaat cctcttttaa aacagcagac catacacggt catctgcgta 102960

atcatccgtg tacaggatgt atgaaatggt gtcatcttca tacaaccgcc acatacctgt 103020

ggcaacaacg aatgtgtttt ttgttttaag tgcccctgtt gtatgatcat cctcaaaaac 103080

agcttccata attgcgttat ctacagtatt tttagtcatc aaattgctcc aacatttcta 103140

agaacgttaa caagttttgt ttgatcattg aatgttcgct tcatatacaa acagtcaaaa 103200

attatactca tccattgacc tacggaaatt ctgcgatcaa taccattgaa gtcagtatac 103260

ttaacaacac ctttcgggaa ccacttcttg tactgatcaa taatagccac ccacatctcc 103320

tttggagtgc tgcactgaga cagtaggtag aatgctgagg catccccgaa cttaccctta 103380

ccgataagtc cttgatacgg cttgatattg tcagcagagt caccacacag catttggtat 103440

ccgaagaaca gcatcccgta accaccaacc tctccattag gcttcatgaa gatgttcccc 103500

ataccgttat cgatcagcat aggggtttcc cttacaaaat ctcccttatc gtttcgcatg 103560

ggatcgaaca caagccccgg tagtgttctt tggtccttat caatagagat aataaacttg 103620

ttgaacttac ctgttttttg gtagtggttc caaccttcaa attgctggat agctaaccag 103680

tcgtcagcct cgataccgtt gatcacttga gcattatact gcttttggag atactgtctt 103740

gcatctccaa gcagcagtgg tcgaagagta ttgttacggt tggctttata tctttccggc 103800

atgggaagat ctagtctgaa attacctgtg cctccaagta cccccacgca ccgatcttcc 103860

ggaataccga gataattaag gactgcatta gctttacact taattgtgtg caagcagttc 103920

tctacaggat caggggtttg gatatccaag acatcgaaat cttctcgtgt ccacggaata 103980

aactccttgc cagccttttt acatgcagct tcacggttgg tattctcagc ttttaaccaa 104040

ccaccaactg tgttcttctg ccgcccccag aactctgttc gtgttttaaa catcttctta 104100

cgtccggagg ctttatgtgt tacctcaatt gtcctttttt cacaacttgc tgcagcttga 104160

tatgcaacct gatcgaagtc ataatagact atagcgtcag gttcaagaag atcggcaaga 104220

caatctatgg actcaataag tggatattct ctactcaaag accctcctgt tagttattct 104280

gcaagatggt tggatgctgt aatggaatgc atcatcgctc tctgcaataa gcgactcaac 104340

atcccaatat ttttcaaaaa ggaacggatt agacccccag tggtgggtta ttttcatcaa 104400

ggagtctggt atcagatagc cacagatctt aattacatcg ccaggttgaa tggtgttttc 104460

atgaaggttg cttgccttct tcaggatcat acctttatga ttctcgtttg catacaactg 104520

gacaatatct ttatcttcaa caataaaaat tttaagcata tctagttctc ctggtttata 104580

aatatctgac tgataatatc agattaataa cacttttgtc aagacttttt agtcttcctt 104640

ggagagcttt ttgttgatct tttccttttg tggtagattc caagtgcagc gttttcttgg 104700

gcgcacgttt taacagttct cagacaagaa ctttcagctt taggaaggtt tagctcccac 104760

caaccaagcc agtaaagttc tgattcagac aactgatcag attcagcctt ccggtacaat 104820

gattgcatac ctctaataat gtcgtccaga ggtgtatcga atacaaaagc tgtcttatca 104880

aatttttctt ttacaggttt cttagccatc aaggtaaccc tctatccacg aaaagtcggt 104940

attccattcc ggaactttac taagctcccg ccactctgca aagcagtctc ggtaataagc 105000

ttcagccatg cccttagtga tcttatttac cttagccaac tgattaactg cctgagcata 105060

tttcccacgg atatttgcta acccaggatg caccgatgtg tgacaatctg ggcacagggc 105120

tatcagtccg acaagccgga tcaccccctc ttcgaaggtg taaagttcat gacactccac 105180

agggtgtttg ctacctttgc caccacacac ctcgcaagtg tagttggctt ctctgtagca 105240

cttcttacgg atcttatccc actcactctt cttcacagcg tttcggaggt tattatacca 105300

agctgtttct gggatcagtt gcacaataag cctacgttca gacttcatta aacgtccgtc 105360

ttccacgtat cctcctcatt caaagaaatg ggtgcgaggg caggaacctg cctcaccttg 105420

tttcacaagc tcatatagct caccttggct tgtctcataa aggcaaatac gtttaccatt 105480

gtcccgatat acatctacaa ggtacgctgt ctcacctgca aacacaacac cacagacaag 105540

gcatgctgcc atgaacaaaa ttgccaacag aaaatctttc atggttaaac cctcctgaac 105600

attctatgag gaaacaatac cacatccgtg tggttatgtc aatgattaat ccaggacaac 105660

gccgcctgac tttttaaaca cctcgatcaa cttttcgatc ttctgttcgt gctgaccgta 105720

ccctgcacct ggaaggcttg cccatcggga tctgcaaaga tggatggcct ggtgaatacg 105780

accttcatca atatcatcga gcgccttaca ctcacggatc agttgcaacg caatcttgtc 105840

ctgactggta gggccaaaat ctggtaatcc taactgtttc atatagtgtt tagcaaactt 105900

ctccagaatc tggtaacgcc cagcagcaga ggaggtaagt ttagcattta gtttaacgta 105960

aacacctgga tgcttactgt aatcgctaaa cagcttgccc ccgactatga catcgtagcc 106020

ccgatccttg gtaggttgtc taccattatc tgtaccttct gaatatgcga tcatatccaa 106080

gaaagctttt cgttgaacat ttttatcaag tttcattttt catactctcc agttcactca 106140

taacgagcca tgatcagcac gtggctaggt tgcacagtgc atacatcagg gcatatgtag 106200

gtgccaattg aaaacactca ttcaattggc taccgttatc tactaaacat acctacacta 106260

ttccagaacc tcccaagtgt ctggattaac atttcggacg cagataccga cagggaattg 106320

gtagttgttg tcttctgtcc ttgcttgata tttgaaggtg acaaacttgc cgatcatttc 106380

ctgcatcctg tcatagctac gctcctcgtg cgtacctttc atcttcattt taacaacaac 106440

accttccttg tcaacacaga taagaacacc ctcatcattc ttatctttct caacgtcaat 106500

aactttcacc tctgcgtcgt tgaacttttt ccatttcaat gcttcagatg ttcgctgacc 106560

gtattcgtat ttaccacaga agttacgcat gatagtacct tcataccctt cggacatgaa 106620

tacaccaaca gattcttcgc aatgatcaac tgtgtacgca atctctgcgt caacaacctt 106680

gatatggtca agcatcagag tggtaatggt atcttctgca tccataagat ccttgcagcg 106740

tccagactct gtttctggat cccaccacac cttgtcacta gggatatcga aaatgtgata 106800

ctctaagtca tagctacagt accctccata acgcaaatca tcacgaacag gctcaacatc 106860

aacaggaata tctaccttat tgaagttttt gtatgttgat aatcctgcct taattgcggc 106920

atgacggcgt tttttatctg cctcgtagtc tttatcgatc tcggcctgaa tctcttcctg 106980

tgtgcgccac cgtttagcaa gagacactat tttctgcaga ggcagaccat gaatatagat 107040

ctctccgtcg agctgtttaa tcccggtggt gaagtggaaa gaacgaagtt gctccagaag 107100

ctttccttga atagggtagg acttattacc acgggaaata aatttaggct caccgttttg 107160

gaaaacaacc agtagacgaa ggccatctaa cttacgcaag atccagcaag gtaatttaag 107220

tgcctttcca cgcttcaggt agtcatgcac cagcatgggt aaaaggtcaa caccctgctc 107280

gcaatcttcc atattttcaa cataccccag acggacttgt ttttcccact tactgatcgc 107340

ttccagctca gcctgttgct cagctgtagt ttcattggca cgacccacgt tcttaggtgt 107400

gcagatcgtc tctttgtatt gcagtttacc atcaagcttg ccaaactcta cgataacctt 107460

atcaccatcc gtgaatactt tccactgttg gtagctgcca tctttgttca gtgcgtacag 107520

tgttgttttg atctgatgca ttactcagcc tcttccttaa aaattttaaa catctcttct 107580

ttgctcatta ccaaccagtc tcccataaag tcaactaacc aatccccttg gtacaagacc 107640

aggttaccaa acggctcgac aagctcacac atgcgaagaa tatctctggt tcgaacaggt 107700

gacgatgtaa acacacctgt atcaatccac cgttgcagat cagatactga tgaagagctt 107760

ccgtcaaact ggtgtgcttc tacaggttta gcaagattaa ataacggcat tgcagtcctc 107820

cttctctaat aggtctttta tcgggtaaac aagcaggtgc ggatcgtaat aacttatagt 107880

cttattctgc cacactatga acttaattac aactaaattt accacatctt tatccccatg 107940

tagtccaaca cacaaatttg tccatatttt gtggttgaat actttgctgt cgatgtagat 108000

cttagtgtca aacttatcct ctatcctacc tataagttta accaattctt tcatctgatc 108060

accaaacaca gcatcgtttt caagatctct agagtgcttg tacataactt tctacctcac 108120

ttgcatactt tccgaaacaa gtaaagccgg gatcaccccg gctatgttat taaatatatt 108180

ctgttacatc cttgaagctc gaaggcttca taatcttacc tttactgtcg aggaatatga 108240

taactttatt tccgttgtag aattcttctt tagcaacaaa accttcatag cgaccatcaa 108300

agcgtcgctc aagatcttta atctccaact cgatgagttg ttcagcaggc atatctgcac 108360

tggcaccgtg tgaataccgc agatggttga cgcgtgcaaa cttgctgtca ttagatttta 108420

gaaccttccg catcatcccg cacacatcac cgcgaagctg gaacagcaaa gctgttgaca 108480

actctgctaa gcggtaagca gacgctcttt gcttagaggt atttttcgca gcgatcagtg 108540

tatcataaaa ttcaccgatc ttaaacttaa gacctcgcgg attatcaaac aggtttgacc 108600

acttttcatg gttatcgtta acaagataag cggcataact tgtgacaacg tgaagatcgc 108660

aggcctcttt taggagatga tccccaccat cacctttctc caactcttcc aaaacttcat 108720

tgaactcttc ctcaatgaaa ctcagctgaa gttcgaatgg tgtgtatgat tttttcactt 108780

tagctgctac acggttccaa ttttccaccc agttggcaga ccaaaggtag ttttcaggct 108840

gagacagttt ttccaggttg tcgatcagtt tagtattcag cattaaaagg cctccgctta 108900

cagttccata aagttttcta cgatgtcaat gaatgtttta tcagagaaag cgtcaaccgc 108960

cccgttaaac caaactggga ttccgcgatc ctcatactgg taaatgtcac tacgtgaaat 109020

gccattctcc cctaaatgct gtgagtcgta tttgttaacg atcagtagtt tagtcccctt 109080

attacaggca gggaatagct cccagttaaa attgataact gtgttggagc accctacaag 109140

gataaccata tcattgctcg tcagagaagt caacaggtca tacatgtcta catactttgg 109200

agctacttct ccgaagaaca ctacattagg cttgacccaa gtatactcgc ttgcatctac 109260

ctcagagtat ccaatgtctt taatgacgtc attcccttca tcatcctcat aaactatttc 109320

tggcaaatac ccgtggacat gcaaaatatc ttcatgtttt acaccagcac gctccagcaa 109380

atcatctaca ttggtggtca ggttgatcac acgaccagac cagcgggaat accactcagc 109440

gatccggaga tgtgcaaggt taggctcaac actccccaaa gctacacgcc gtgcattgta 109500

aaagtcatgt accttatagt agttcttggc aaaggtgtgg atgttacaca cctcatcgat 109560

gctgaaattc tcccacattg cagtgccacc agatcggaaa gttgggatac cagattcagc 109620

gctcaaacca gcaccggaga taatgatcag tcgtcgcata agtgtcctca gtagatgttt 109680

tgtttaattt ctattccaaa ctttttaaac gagtcagcag caagcatgaa tttacgagcc 109740

gctgatgggt tagaagtatg cacgaaaatc ttcacgttgt caactacagg aggcttacca 109800

aatacaaatt tttcctccag tatgcagaag caatcgtatc cttcaaaaat ttctcccaag 109860

tcgctgtcaa aatgccactc agaaacgcgt ctatatagct ttcccttgtc attaattaaa 109920

tccatgaact cagaagaact ctttagccaa agagcgtctg ggagcccata atttgacgga 109980

tctctcaaat catctagcca cacaatcagc ttcttcattg catatagtcc tcgttgtgga 110040

aatagcagtc aagtatctca cggcgttcct gctccaggag gcaattccac acatcttctg 110100

tgtagtttct acgaatgaag tttaccatgc cgtaaaactc ttcgtcaaca cgtttaaaga 110160

tatcatcatc ccaaatcttc caagctctac ccctcattac gaccttccgt gccagatagt 110220

agggagactt gatctttagt tgcaggtcat aagggtagta aggcttacgg atcatatacc 110280

cttcgcccct tgtttctgca gccaactctt ttgcatagct gaacggaatt tcacgcacag 110340

tgtgaggacg ttttgcacca aggtatttgg caataccgtc taaagctgct tctggtagca 110400

ttattccaga atcattgtat cggcaaccga tcaaataggc accatgatct tcctgaacaa 110460

tatgcgggtc tgaagggtga caaatttcaa acatgaatgt catgttttca tggcccataa 110520

ccttcttgat aagatctaaa ttaccacatt gctcctggat tgtttccttt gcgaggttac 110580

aatatggaga atcaagagac cctgttgttg agaacaacag ttcacctttg tgagatgata 110640

ccacaccaag gaatccgttc tttttctcta ccgccgtcac aagtttattg tcaggcaggt 110700

tggtggaatt ttctcccaag ttaaacactt ttgtgaaggg ccatgccaca acatttcctt 110760

cattaccgac aacaattcct cggcactcta gtaaacgcgg agacaaatgc catagattgt 110820

tgtaaaatac ttttctggca tatttataga tctttaaccc aagatctgga taataccgac 110880

tggttacaag acctttattc actaaatcat ctgcattaaa atacttcatc atacattcct 110940

cgtttgtcat tttgtcactc tatcttgcca cacaaataaa ctagtgtcaa cacttgaaat 111000

gaacaaaaga gttatgtata ctaccattaa aattaaaacg gaggattact atgtctaaaa 111060

tgctatatat cgtgcgtggt ctaccaggtt ctggcaaatc aacactggca aatgcactat 111120

ctttagatgg gctgtaccca cattttgaga acgacatgtt ctggtacgac gacgatggaa 111180

attacaactg ggatgcaagt aaagtaacac ttgcagcaaa atggtgcaaa gataacgtag 111240

aagatatgat gcgtatggga tacgaccgtg tcgtggtaag taatacgttt acaaaagaaa 111300

aacatgtgca agaatatgtt gacatggcaa tcagtcatgg gtatagttat actgtgatcg 111360

tggttgaaaa ccgccacggt aataagagca ttcatgatgt tccagatagg attgtggacc 111420

gtatggagcg tgagcttaaa aactgcatta aactaaaatg agggatgtat gaaatatttt 111480

gtaacaagcg atacacactt tctccaccag cgagtcatgg agtttgattc atgtcaaaaa 111540

cacagatctc agtatgatca caatcttgac agtatgaacg aaggaatcat tgcagagtgg 111600

aactctgttg tatcaccaga agacactgtg tttcacttgg gcgatattgc atgcactaag 111660

gataagaata tcaacaactt atttccagat attctaaata tgttgaacgg acatattatt 111720

cttgttcgtg gtaaccatga cacaaggacg actgtcagaa ttatgaaaga gttcggtcat 111780

gaggttgtag attattacga gcttcgccac aacaagaatt tgatctgtat gagccattac 111840

cagtttgcaa agtggaacag aaagcctcac ggatcagtcc aactgttcgg tcatgctcac 111900

ggaaccacac agcagttgtt tggcaggcag tttgatgtcg gttgggatgt gtgggggaga 111960

ctcttaccat tagaagaggc ataccgtatt gcaatgagtc ttgctattga cagcggggag 112020

taatccctgc tattttaaag aaaattgcca cacaaggaga atcaggagat atgaaagagg 112080

aggataagct gccatacctt aaagtcccat ttggaatact gtctatgtca aaagttttga 112140

atgatacaac tggagaggtt gttgttcttg acttgtatga caagatcgta tactgctacc 112200

tgcacaactg gtataagctt ggaggaaatt cacgcttcac acccagcaca aggaaaattg 112260

cagcagatgt tgggataaag aaaacaaaag cagcagcgtg cgttaaaact ttagaatgtg 112320

caggagtggt tgaaatatcc acacaaggga gaggtaaaag atgcacattt tccaaagttt 112380

tgccaccaga agaggttatt cgaatgtcaa caactggtta gaccagtaaa aacttaaata 112440

tctgtactat aaggattcgt atgttttatg tccatccaag agcagagggt agtaactgtc 112500

cgtaatacgg acactactgc ccgtcacgcg gacacatctg cccgtaatac ggacactaac 112560

tgtccgcaat acggacacat aaagaatata actaaaataa ctaaataata aaaaataaaa 112620

gatctagtac ttatggtact aaagtacata tacttaactt atgtagaata tactagatcc 112680

taagtaccat gttttaaatt tattacttac tattggtagt gtcggccctt cattggcact 112740

cgcctacgag gggcgagatg ccgggcctcc atagtgcaca caataacagg agtgcataca 112800

tgcaggactg ttaacatgtg tgggagcgaa gcgatagcgc aggccaaagg ccgagcacta 112860

atgtggacca ccgacgaaag cttgccagcg ccgtaaggag tctgttgaga gcctcccgcg 112920

cgagtttggt taaagtttat gtgtggaaac ggacaggaag gcacctaaat cgccacacag 112980

ctgcgtaaac tataccaagt aaggcaatcg caccagtttg atagaaaagc tcgttacaaa 113040

gcgatacagt gagtttttct ataacttgaa gagggggcag ttatgagttc acgcaaagat 113100

gaccgtgaaa agattgcaaa acgttatcag aatgaagaac agatggagaa aactttcaag 113160

tggaagccag gtttccagcg taatcattct aacgctcgtg atcgtaagca tccggataag 113220

tggctaacac cgccaaatga tggcatgcag gttggtgatg agaaggagct atcgcctgga 113280

aaagatggca actacattcg cactaaggac gctggtccaa aggatgacag ctatcttgta 113340

gataaggttc ttgatggtat tgatgtagat gttgacacca cacggaactt ttttcttggg 113400

tggtatcgca caggtttttt cgacatcaag gccattcgtc gtgtttgtga tattaaaaag 113460

tctgcatatg atgacgactc gttacatcgc accctgaggc aatattcaga aagatatggt 113520

catccggtta tcactcttga cgatgttgac gaagtgatgt atcatgcaga ggttgttgca 113580

ggcagattcg gtgatgcata tcttgatatc ctttctgcaa acgatgaggt aatgttgaat 113640

aagatttact acgaacgttg aggagagctt aatgaacaat ccggtagcaa agaactgtaa 113700

taagttcaac aagtctgcaa ttcacactga caggaaaaag agctggtcac cggacattga 113760

cgaggagctt gagaattact ttgaagatct ggttaccaat gcaggtgagg ttgcgattaa 113820

agaatacgca gacaggaggg ataaggccgg aatctgttga caaaaataat gttacctgat 113880

aagatctgca atatgatgtg atacaatttt aacttacgag gtctacaggt aatgctatgt 113940

ttaaggttaa gctcgttttc cgttccatga atgaggatta tttagaagat gaaggtgaat 114000

ttcgtgcact agatgatgca atagattttg ccaacgactt agcttgtatg aacacgggtt 114060

atgggttgtg ttgttgggac ggtggtattc agatcggtga tagcaaaatc ttctacttct 114120

accctgagcc ggataacaaa caaattaagt atgttttatt tatttgtgct ttacaatgag 114180

gttgttttcg tgtatcatga tgaatatcca atttaaagat gagtttgatg gctttatctc 114240

cggatgcttt gaaacagggt tcggagaagt ctgagttccc cttagagggc ttgatagtgc 114300

accacactta aaatggtagg ggcagccagc acatgacgat gtgaccccag ccaacctgta 114360

ataaggtcga gccagagcgc ggtaatgggt atctggtaac ccacttgttg ggtcttgctg 114420

gtccaggacg ctgggcaagt cgcagggaat accaccctgc ggcgaacttg acaatcagta 114480

tttgccgtga taaaatccac ctatcacgtg ataatggagg aaacatggcg gatacaacta 114540

aaaagcgtcg ccgtagaggc agacctctga aagaggagtc caacgccatt cagctagaga 114600

agaacattct tgatatgaag gaatctttta tagcgatggt tccagaagct cacaaagttt 114660

taagagagct gatgttggat ccgaaaacca agcaaaatat tcgtcaaagc attgcagagt 114720

atgtccttga taaggcagac cgaatgcagg aagaatatga tgagatgttt ggtgaagagg 114780

aagaggtggt agtagaagag gctgaaaaac cagcaccttt tatgccattt accacagaga 114840

tacagcctgt tggcaatagt taaaatattt tcttgacaaa ctttgcttaa cagtgtaata 114900

ttgtaaacat ggtctcccac cttttctggc ggctcaggtg taaaagaaag ccacccagtg 114960

ccctgttagc ttagcggata aagcagcggc cttctaagcc gttgacactg gttcgagtcc 115020

agtacggggt gccaaattgg ggagttatcc cgtagaggta gcggtgtaga ctgtaaatct 115080

attgtcattg cgactcgggt ggttcgactc caccactccc caccaaattt atatttttaa 115140

atgcataaat catacagata atgacattga tatttatcgt cgtttatact agtcactaac 115200

tgctcgaaaa tcagtgtcat tatcagtgta atttataaag gagggtaggg agcaatggtg 115260

ctcaagcggt cttgaaaacc gtcccgttga ggatgactcg atgatggttc gattccatta 115320

ctctccgcca cattgttgac cttgtaatta agcgaaagtc ggaagcagaa ccggattatc 115380

aaatgttaca gtcgtcatcg ccgactagcc acatggtcaa catattgcgc taggtaagcc 115440

ctttggcaag ggcgaggatt tttaatcctg aaaatagacg aggtagtcgt tgaatacata 115500

ggtggttcga ttccatcacc tagcaccttt tgctgatgta gcacaattgg cagtgcaatt 115560

gatttgtaat caataggttg caggttcgag tcctgccatc agcaccatgg ccctgtagct 115620

ggaaggttca agcaagcgac tcataatcgc cagacggtgg ttcaattcca cccagggcca 115680

ccaaataata aatactccgc gtagctcagc ttggtagagc gcctgatttg ggatcaggag 115740

gtcgagtgtt cgaatcactc cgtggagacc acctttagtt atactttaaa tacatacaca 115800

caaaaggaac ctttaaaatg tatacactta aaattgtaga acgtgtaaat ccaacccata 115860

ctgtagattt tcgtgacgat ttcaaccctc ttgctgtaaa tcttcgagag tcgtatcatg 115920

aactaagctg cgatgcagtt gtggtcttct ttgaagacaa ctctgaaggg gcagtggctt 115980

gtgttgttga tagcgagcag aagttttata tttatagaga tgtagatgct tacttgatga 116040

atgagaatgc ggttactgtc agaatcatcc accgagtgcc tcgataagta ttagtctcct 116100

tttagttagt ttcgatctat ttaggttact caattttaac ccgccccgtt agctgtatag 116160

ctgcggggtt ttttcgtttc tggagcctta aaatgatttc agttttaaca ttgatgagta 116220

aatacagagg gatcataaaa ggtttagttt ttgcagctgc tctaggcaca gcatacctgg 116280

gggccatgta ttggtttaac tctaagctta actctgctta tcaggaaggt gtaacagcaa 116340

cagagttgaa gtgggagcag atccttgacc agcaacaaaa gcgagccgac aggattaaaa 116400

tgggacatgc cgaggaggtg aaaaaccttg aaaaatccct ttctgactta caggataagc 116460

tggaggcagc taagatggca ggtaaagaaa agcagattat ctaccttcag tcgcctgagg 116520

gtaagaagtc aacactacct gaccagctta tagatattta taacgagagc attaatcagg 116580

agggtagatg agagccgccg tattgttagt tacttgcttg ctgctgtgcg gatgtgcgac 116640

aagtgtcgaa aaagaatata agattgatcc tccggatgtg ggaagattga ttgttaaacc 116700

gcccgaaggt gctatgaagc ctcctgctaa agctatacct ttaaagaaag gtgcgcctga 116760

tgcggtgaat tctggcatta tgagggataa taacctgtct tgcagtaatg atagggctaa 116820

attgatcatt ttgcaagact atattagaac actgtttcca aaggagagag gttaatgtct 116880

aagatttacg gaccgtgttc tgaaaagcag aagatgatcc ttgaaaacga tgcagacatt 116940

cttgtcattg gtggtgcagc gggatcaggt aagtcgttcc tcctgcagct gatgccgctt 117000

aaaattattg atgacccgca cagctctgtg gttatgttcc gaagaacaac cccgcagttg 117060

gagggtgaag gaggcctatg gcctaaagca gtggctatct acagcgatct ccctgagaac 117120

atcaagccaa aattcagaga gaaggatcat aaatttattt ttccacggtt tgatccgagc 117180

accggtaagt gggacaggag caaaaaaggc gctgttgtaa aatactgtca catggaatat 117240

gtccaagata agctcaacca tcaagggctt gagtatacca tggtttgctt cgatgaggga 117300

acacagttcg aatgggagca gatagactat ttgatgtctc gtctgcgatc tcagtcaaag 117360

tatccatcaa gaatggttat cagttgtaac cccgactcag aacacatgct tgccaaactt 117420

gttaggtggt ggttggacga tgaagggtat ccagacccgg agaaatgtgg taagaaaaga 117480

tattttatcc gacgcgacgg tgagtttatc tggggagact ctaaagagga gttaatagag 117540

aaatacacca cggttaacgg actgggtgtg gagatcaagc caagaccgtt gtcattttcg 117600

tttataggcg caacaatttt cgataatcct gtctgcttga gagacaaccc agaatacctt 117660

gctttccttg aaggtctacc agagcttgaa aaagcccagc ttcttcatgg caactggttt 117720

gccaaacctg aaggtgcaaa ctatttccaa agatctttcc ttcgtgatgc cgatagagta 117780

cctcttggat cagtatcttg cagggcctgg gataaggcag gaaccgagag aacttccggt 117840

aataaatttc ctgactttac cgccagtgtt aaggtaagta aagatagcga cggcttttat 117900

tacctgtctg gggattattg tcctgagaac gtggatgacg gtaggtattc tacaggtctg 117960

gaaggtaagt tctgtaaaaa ggctggggaa cgagatgtga tcatcaggaa acaggcgcag 118020

tttgatggag atgattgcat aatcgtattt tctgtggacc ccggtcaggc aggtaagagt 118080

gagttcttaa cctcgtccag atccttgctc gccgaagggt tcagggttga agaagatccg 118140

atgccttcca acaaatctaa actgacaagg ttctcacctt ttgccaatct tgcccaacaa 118200

ggaatggtga ggattgtaaa atccagtttc catcctgaca ctctggaagc atttctgact 118260

gaacttgaaa agttcaacgg ggaaagatca acatttaaca gaaaagacga ctgggccgac 118320

tgtgtagctt ctgcgattaa tttcttggag aaagaggagg tagcactgcc ttgtgtgatt 118380

ccatcaatct catcaccttc tatgtttaag cgttttatct aagtgttgcc aatcttaatt 118440

agatgtggta gaatagagta atcggagata ctagccctct gacacgaggg ctattttgtt 118500

atactgtttt gaggggtaaa gcttgtgtcc aaaaagaacc gcaggaaaca caacgccaca 118560

gccaaaactg tgaataaatc tgggaacgca ggcgcaccaa ctcataatgt tcgtatgagt 118620

gagatcggtt ctggcgcact ttcccaaatt atggcagagt cacaatctat gatggttgag 118680

gaattgcgtt ggcctcagct gattgcaaca gtggagacta tgaaatgtga ttccacagtt 118740

gctactgcct tagatacaaa atatgttttc atcacgaaag cattcaatga ttttaagatc 118800

ctttataatg ttaaaagtga agactctaag aaagctgccg agcttataga gtattctttg 118860

cgtaatttgg caaaccagca aactcttcga gatattgcac gaagtgcagc tacctttaat 118920

gagtatggtt tttcactgtt tgagaaagta taccgcagag agaaagaagg taaatacgcc 118980

gggatgctcc ttattgataa aattgcattc cgacctcagg ccagtttgtc cagatcagag 119040

ccgtttgtct ttgacaagaa tagtagaaca ctgacaggta tttatcaatc acctaatgca 119100

tttctaaaca cgcagaatgc acgctgggct ggtcctcttg ctgcaatgcc ttcaaaaggc 119160

tttaatgagc ctgaaatttt catccctgct aaaaagctta tgctcatgac tctgtcagga 119220

acagaatcta accctgcagg tgtgtcacca atgataggtt gctacagatc tttccgtgag 119280

aaagtcttga ttgaaaacct tgaggtagtt ggctgctcca aagaccttgg tggtgtgctt 119340

gagctgaaaa ttccttccaa tattctgaac aaggccagca ttgacccgaa ttcaatggaa 119400

gggagaatgg ttgcagatct tatgcttgac gcagcaaatg cccatagtgg ggagcaaagt 119460

ttctttatac ttccgtctga tagagataag tctggtaaag agctttactc catgaccctg 119520

aagggtgttg acggcatggg taagcagtat tcaactaaag atttgattga tgcccgtaag 119580

aaggcgattc ttgaccgttt cggtgctgga tttattaacc tcggtaacga taacgttgga 119640

tccttctccc tgtctgagtc aaaacagtct atccatgggc attttgttca gcgtgatata 119700

gatattatca ctgaggcctt caataaagat cttattccac agcttctggc attgaatggt 119760

atcatccttg aagatgatga tatgcctaaa ctgaaacccg gtctgattga agaagtggac 119820

atggagagct tctccaagtt tgtccagagg atcggtgctg taggttactt gccaaaaact 119880

cctgcagtaa ttaataagat tcttgaagta ggcgggtttg atgaaagatt tgacgaggac 119940

atggatcagg aagagctaat gaaactcttg ggccaggaca tcagtagagc tggtgatggt 120000

atggctgcag gatccactgg caacggcact tctaagatgt catcaaccag agataattcc 120060

atatctaaca tggaaaatta aaaattatca acacaggggg ttgcaaaacc tcctttggcg 120120

tgataaaata atccacatgg ggaataagat gccacaggaa gaaatgattt cctacgaaat 120180

catctacgaa cctgacacta aagatgctca cggtgagtgg atgagtaaag acaccatcaa 120240

aaaggctaaa gataactggg acgcagcata cgccgcaggc ctagtttctg aaaacctttt 120300

tcacttgacc tctacagatg ctttcaccat tgaaaagacg tggattcaag aagagtttga 120360

tgttgtagtg attggcactg agcaggtcat caaggctggc tcttgggttg ctaaagttaa 120420

atacaataat cctgaattat gggaagctaa gaaggctggc attgttggtg gcctaagcat 120480

tcaggcaaca ggtaatgttg atgaagagac tggtgaaatt accaacgtaa acttcggaat 120540

cagtgttgta gaagagggca atgaataatg tctgaaaaaa cgattactat taaagagaag 120600

gggattgccc tgtgccataa ggcgcaaggc tatagcgcta acaacagacc tgtatctctt 120660

ttgatgaaga gtgatcttca gcctgagcag ttaacagaag atattgttaa ggcacttcgc 120720

caagttactg tggagcttag ttttgaagaa tacctgcgtc gtttcttcga tatgtggtat 120780

gacgatgcca aggcacttgc acacatgctt ggttttgagg ttgaagagga agcttgggct 120840

aaagagcatc ctgattggga atgggcgcaa gctgacgcag agtcttgtat tgcatggctg 120900

gaagagcgtt gtgataacgt gaccattcat aaagctgcaa aagaaggcaa gcagcttagt 120960

gtggtagatc agtacagcct gttaaaaaca caacagattt ttgaacaagt aaccgctgat 121020

ctcttcgata aagaagggaa cctgatcaag gcggtacagg aacctgctga ggtagataaa 121080

actaccgagg tggatgatgt tgcaaaatcc gcaaataccg aggagaatcc ggtggacgta 121140

accaaaacta aagagtatct ggatctgctg aagcaagtag aagagctgaa agcaacccag 121200

agtaaagctg aagaaatcat caaggcccag gttgaagttg aaaaagctaa aatgctgact 121260

aaagctcaag ctttaagctt tgttggtgaa gaagatcatg caaccctggt tgacttcatg 121320

ctggaaaaag ctaacgaacc tattgtagct atgctggaga aagctcaagc caaaattgaa 121380

gagctggaag cagaggtgca taaaaccaaa gaagagtttg ccaccactga acatggtaat 121440

gatggcgaac ctgttgtgga tgatattgca aaatccgcag aagaaattct tgctgaaaac 121500

gttgcaaaag cacttgctcg tgcacgtgca gaatctacca aataagacat taacggggga 121560

aataaatgat taccgctcaa tattctgata tcgtgctcgg aaaagttgat tctagcgatg 121620

ctggctacaa ttttaaagag atggaaatta cactgacaga gcatcaccac gctggcacgg 121680

ttgtgaccaa agatggtagc attgccaatc aggacggttc agacgcttac ggcgttctgg 121740

tggatcgtgc tttaattcca gactctgctg gtaaagtgca tatcgctgaa cctcttggca 121800

caaagaaata taaactggtt gttgccgtgc gtggtgttac ttttgccaag gataaattgc 121860

tggcaactgg tggcgctaaa gcagcacagg ctgtgttaga caagcttgag gctctgggta 121920

acaaggttca agtccagacc cttggtgatg agtaagccct aaattgttcg ggaaaatatt 121980

taagttttac tttggagaaa taaatgatta ctcgtaaaga tgaatttggc attgttgatt 122040

taggtgctac tctggacctt gttccgcgtc aatttcgcct gatcactggt atggatctgt 122100

tcgaaacccg tctcggcacc tcaacaattg ctcagatcga gcgtgttgat gaagttgtaa 122160

ccgacattcc ggcacgccgt cgtggtggtg agcgtaacta cgttggtagt gaacgtgctc 122220

aggttaaaaa cctgaacatc ccgttcttcc ctctggacaa aggcatcact gccgcagatg 122280

ttcagaactt tcgtcgttac ttcacccctg atgctccgaa aactgttcag gacgtagtaa 122340

cccgcgttgt tcgccgtatc cgtgtctctc atgaagcact gcgtgagaaa gcgctgttcc 122400

aggctattct gggtaaatcc tatgcacctg gtgacacaac ctgtcagtat gactattaca 122460

ctctgtgggg tgtgactcag aagtctgttg agattgaccc tgcaaaagcg gatcaggatc 122520

ctatggaggt tctggaagaa gctcgtctgc acatcgcact gcaagctggt gataacgcag 122580

ctggctatcg tattgcagct ctgtgctcac cagagttctt cagcgctatt acccatcacc 122640

cgctggtaga actggcatac acctactacg gctctgctca ggaacctctg cgtcgccgtc 122700

tgggtgctgg cggtcaagac agcgtatacc gtgtgtttga acataaaggt atcaccttta 122760

tcgaagatat ctctggcaat attcctaaga aagaagctcg cattctgccg atgggtattg 122820

atcagatgtt ccagctgcac tttgctccgg cagatgatgt taacgaagca aacaccccag 122880

cacaggaact gtacatgtgg tataaacact ctgcatacct gcgtgaagag aagattgaat 122940

ctgaaacttc catgctggca gttaacaccc gtccagagct ggttgttaaa gtaactctga 123000

aaaacggtgg ctaatcacta aggtgattca tcccatatgg ggagggggtt ctccccctcc 123060

cctttttatt tattagggtt taacaggagt gtcccgtgaa gagagccgta tatccagttg 123120

agtatgatcc tctattaaga gttttcaaca atcagttaag tttctatcaa tttttagcac 123180

aatataaccc agaaatcaat gttggtctat cagccttgct gccaccaaca gaattcgtta 123240

ttggcagtgt tcactatcca tgcagaacta ttgtagactt cttcgagaag attgcaaaac 123300

atacaggcct gccaattgat actggtattt caaccatccg tatgggtgtt attcttctgt 123360

tcttcacaga gaaccttgat gaaagcactt taacactgca aagcggagat gtcaatatca 123420

cactcactcc gtcagtgttt aaagaggtag atgctgcttt caaagtttcc gtggcagaag 123480

gacatctgtg ggaccctgca gggatgccta aaaaagtgac cataaacgat gagcaatgca 123540

gtattgttta tgaggaagat acccaaactt tcaccatcga atggcctaag aaacgctctc 123600

aggttgtaaa tcttgggctg acttctacag tggcccctga gcctgagcca cctgtaggcc 123660

ctactgagcc cggtggtggc agtgaggaat cacagggtcc gaccgcacct gagggcacgg 123720

aaggtactga gcaggatcct gaacaggcac aagaatcaag atcttctcac agtgatactg 123780

atgccatact gcagcaagcc gcagctctta acaacgaaga tgacaaaagt ggttcaaaag 123840

atgcactcca aacctttgct aaagagtacg gtgtcacctt aagtaaggca aaaactttcc 123900

aaaatatgat gaaagatttt gaggctgcac tggcataatt ttgttaaggc cccttccaag 123960

tcaggaatgg ggctttattt ttacgaggta ttttcaaatg gcatggtatg aagatgaaga 124020

gattatcccg tcccctcctg accatgatgg cgtagtcgat accccagaag aaccaccaaa 124080

agaagatcct gcaccttctg aacctgatcc agaccaacct cttattgata ttatgaggtt 124140

attgcttggc aatgttgatg aggaaacact ccctgacggg gtaattaaaa cattccttga 124200

catggagaag ctgaaactaa attatccggc agaccctgat aagctacccc tgcttaaata 124260

caatgtgctt gtgcagcttg tgcggtggct gatgatgcag gaagtgtcta gcggtaatgc 124320

aagtataact tcccgtctgg aaaaaatagg tgatgagacc atagagattc gtggtggatc 124380

ttcctacgcc cagtggaaag atttccttga ttggctgctg gcacaccctg actatgtaga 124440

ctctgagcta gatgcttata gcagaatgat tatcattggt ggtgttcgtg tagatgaagg 124500

aattcgggtt aaatttaatg aaaacagtaa cggcccgttc gatgttcagg gaattacacc 124560

tatcagtggt ctaaataatc aacctcctcg ccacccaaga cgcaaacgca gtttttaaaa 124620

attgttgctt ttgatgtatc tttgtggtaa aatacattag tgaggaatat aatgctaaag 124680

gttaaatgtc aaacaaggtt caacttaaag cgtttagatc gcttttataa agaccttgtg 124740

aaacttgaaa gcaaaacaat aacctacggc ttttatgatg aacctcaccc gtcaggctta 124800

aatatggcaa cattggcagc tattcacaac ttcggttgga acgggctgcc tgttcgcaat 124860

ttcatggaga cggcctttgc atttcacagc acgcaactgc agaccttgac agaaaaattg 124920

ttgagagcta tggctagagg atcaagcgca gaggctgttc ttaagcagat gggggacaca 124980

ggggccaaag ctatccagtt tgttattgag gctgggcagt ttagtaaccc tactgttagt 125040

gagcagtggg cacaagagaa gggcttcaac gaggctatgc ggcattacga tgtgctgctg 125100

gaatctgcaa cctttaaaat tggacagcaa aaataagggg gttgagtggc agcaggttat 125160

aagctgatag gaaagaacag gctaataccc cgtaaaacat tcaaaggtcg ccacagagtg 125220

tataacaaaa cagttggtgg tccttttgct aacgaggggt tagaacttga gtatgaagag 125280

tttgatgtct tagagtgtgt tgtgcagccg ttgacgggaa gggcagctaa agactactcc 125340

tctcaaataa atcctgaagg tgacagacag tacgaggctt ttacagttta ctcctcaacc 125400

aagttgtata gtccagatga aggaacatat gcaatggctg atcaaattca actgcctggc 125460

atccggggtg acttgaaatg gtttaccgtt ttaaaatgcg atgcatatgt aacgtctggt 125520

ggtggaagat atagattttt tgtggtagaa gagcctgaag gagaagactg atggaagctt 125580

tcgaaaaagt tttttctgat cttgaaacaa ccattgctaa gcttgtcaag gttgccacag 125640

gaagaactgt agttctggca gaaagctcta ccataccgaa gccggaaggt gagtttgtac 125700

ttttacaaac cttggcgatc aatcccacca cttgggaaga taatgagttt caagatgcgg 125760

agggtaatgc ttacgtaaca cacagttaca ctgttaccta tctgttaaca gcttatcgtg 125820

gcagggcaca tgctgcactt agcagagtat tgcaagcaat aaatcttcca atgttttatg 125880

acaagtattt ccctctggga tcgtgctttg cttactctaa caattcaaca atctcccctc 125940

aaagggtgcc gttgaataag cagacgtatg aaaatagagc cacggtaatg ctgacattta 126000

acgtaaggtt tgttgagaca gacattgggg cttttgagga tcttcaggga attaaggctg 126060

aaataaccac gcacttccct tcacccaatt ctgataaatc ggttacagac agtggtcagt 126120

aatcgttata attgtgtagt gtaatgatta cactgcgtgt ttgatatccg tgcacagata 126180

ttaagaggaa acaaatgcca tatttagata aagtggtaga tgttacagtt aacctcggaa 126240

cccagcctat tgacactgta ggtttcgaaa ccccgctgtt catcgctatc cacaacaact 126300

ttactgagcg agctcgcgta tatgctgagc tggatcagat ggtggaagac ggtttcgcac 126360

aaggctctgc agcgtatgag tttgcagcaa aagctttcgg cggtgtattc cctccacagt 126420

atgttatgat cggcagacag gcaaaagaaa aaaccactgt agattttaaa ggtattgctg 126480

ccgcagctga taccgatgtc gtaatgacta ttgcaaaagg tagctacaat acttctctga 126540

tagtgcctat ctctggtgga acaggtgcaa ctcagattgc agagtcaatg aagactaaaa 126600

ttgaagcaga tgatagcttg aacgatatca ctgtagcggc tgaagacggt gttctcacca 126660

tcacgggtga ctgtactgtt ggttatcata ctggtaacta tacaatcaaa aatatcgcaa 126720

atgctgaaaa accttctaca gtagttgata agatcaatgc tgagcgtgac aattggtact 126780

tcctgtgcca tgaagaccac accgacattg aagcatgtgc gaagtgggct caggctaact 126840

acaagctgca tgtctactct actgcagaag atgttactgg taaagataat aacatcgcca 126900

ccaaactgaa agctgcacaa tatgacagtg tcggcatgta cgatcctcgt gcagataagg 126960

acttcccgga gggtggtatc gtaggcgcta tggcatccaa cgatccgtct tacggtgaca 127020

gcttgcattt aaaacagatg ccaggtgtga ttgcaccgtc tctgacttta actcagcgta 127080

tggcgatttg ggaaaaccac gttaacttct atagaatgat taacggtgtc ggcgctttct 127140

gggaaggtaa gtgtgcttca ggtcagtatg cagacgctat tcgttttgca cactggatca 127200

agttcagatc tgaagaatcc atgttcgggt acatgcatcg ccgctccaat atgggtttaa 127260

gcatgaagat gtctgatgat gatcttccgg ttatcaagtc tgttctgatg aacaacccga 127320

tcaatactgg cattaagaat ggtgcgatcc tgacaggttt tgacgaagac aacaatgtgt 127380

tctacgaccc gatcatcaca gttcctaaac gtgcagaaat cccaaccaat cagttggctg 127440

ctcgtgttct ggaaggtgtg aaagtagaac tggtgtataa caacgccctg cacttcgttc 127500

gtatccggat caacgtcctc ctggacaaag ttggttctaa atccaccaat gctgttgcaa 127560

tgactgaata aggaagacta gatggaaact caaattttaa ctccttatgc atatgaccct 127620

aaaaaggtca aactgtatct gatgcagcag cgtgtcactg gcttcgctgc tgacaccaag 127680

atcgttgttt caagaaacga agacaacatc taccctcacg tcggtgtgga tggtgaaatg 127740

tctgcagctc tgtctcgtaa ccaatctggt gttatgactg tgtccttgca gaacacttct 127800

gtatggaacg cttacctttc tgactggcag aaacaggcat ctattactgg tctggtattc 127860

ttcccggttc tgctggaagg tagccaaggt cctggtattt ctacaatcgg ttggatccag 127920

aaacagcctg acctgactta tggaaccgaa gtaggtcaac tggactggga tatcggtatt 127980

ctggatgcat ggctcaaccg tgacaacatc actggtgctc tcactggcct cgcaggtctt 128040

gcaggtatta tctaagggca cactttagca taggcctcac cttcgggtgg ggctttttgc 128100

ttttatggtt ttgacacacg ttcttgaatt gcttgaaagg ttaaattgac aatgcaccgt 128160

aatcatggta aaatacacaa atatggtgca caacatgact aataaggatg ctataaaatg 128220

gctataaact ttagacctac aacagagatt gatttagcag ggcacaactt tgtgatcacc 128280

cactggggtc cgatgaaagc aatgaaaaac atgcctaaaa tcggtaagat tgtggctgtc 128340

ccgctaggtg ctatcggtgg atctattctc tcaggcggtc agaatatggc agaggtcctt 128400

cctacagccc tggcttacct gttccaagaa cttgatgata actctattga agagttattt 128460

aaaatcttgt tcgaagatat taccgtggat ggtgtggata agcttaaccc tgatgttgtg 128520

tttgcagggc atctgctgga gatgattaag ctggcaggta aggttctgga ggttaactac 128580

ggctgttttt tcacacaaga cggtttagga agccttctgg agatgttaca gcagatgggt 128640

atgattcagc aggtgaacaa tctggatcag actccggaag agcaggaata agcaaggttg 128700

ttgtgagggc cagtgagtgg gcagccaaga acagttcact caactggttc gactttcttt 128760

ggtgcagggt gctcaagaat ttcaaagggg aaaattttga atcgctaaat cttgcagata 128820

tggcgtattt cctgaagctc tgtgagtatc ttgatatcga agaattccta gataccgtcc 128880

aaaacaggga aatggagcgg gagcagaagg ctgccgcagc tgctgcgaaa gctaaacgca 128940

ggagaggata atctaagggc cggattcctt ttctgggatc tggccctttt tattatgagg 129000

aaagcatggc tacaaacaaa atagttgcca caacagtcaa caagattggc tttgagatag 129060

agaactcctc ctacaaaaaa gctgtagaga aaattcgcag tattgggagg gagttcagga 129120

aactgggcga ggattttaac tcagccaacc caatgagtag atggcaggca gtggctgtta 129180

aaacccagca aacaatgcag cgggttgcca gacagcaatc tcaggcaaat gctaaacttc 129240

acagggagag cgtagcacaa gccaagaaag aggctgcagt aagggctgca atagaacgta 129300

gggaaaacgc aagacgtaaa caggctgtag gtaatcttac cgctaaaaat ccagaaatgg 129360

ccaggatgcg taagttctac cagcagcaag ctaaagaggc caaaaaggct gccaaatcta 129420

caccagtgtc ttacggcggt tataaggcac caaaggcaag agttttggct aatgccaaat 129480

ctttcaagta tatcccaggt aaccctaata tgggtggtgt ggcttccgac cctaaccttg 129540

tgaaagctca gacagccgct atgaatcgtt atcatagaca gctaagaaga gacagtaaag 129600

ctggtatgga taataagctt atgtctgatc gtgaggcaag gagaacccac ctctacaact 129660

ctgcgataag actttctgcc aagtatgggg ccggattccg tggaagcttg gcaggatact 129720

cagagttaga gtccaggttt gctcgcgggg agatgaaaaa gtcaaccttt aacgcccacc 129780

tgtcagctct gcagtctgga tttaaaagcg ccaccagcca aacgcttaca ctaggtgatg 129840

ccatgaggga cttacgtagg tctgttatta atgcaacagc tgcgtatact gctttctctg 129900

gatttactgc tgtgaacaga tctggtcaca tgatggaggg ggcaagtgct gctcttactg 129960

ctgttactgg agatccaaac aaagccaaca gtgaggttgc gtttgtccag caagaggcta 130020

tgcgtcttgg ttttgatctc aaatctggtc ttcaaggtta cgcccagatg gcggtaaacg 130080

ccaaaaacca gatgacaaat caagaggtgc atgagctatt ctctgcatac tcacagtatg 130140

ctgctgctta cggtgccgat gaggtaaaat accagcgtgg catcatggca atccagcaaa 130200

tgctcggtaa aggacaggta atgtccgaag aactgaagca acaacttgct gaagcattgc 130260

ctggtgcata cgaacctttt atcaaagcca ccaaagaggc ttttggtctt tctgaactat 130320

ccatggatca gttcatggac atgatgaaga aaggtgaggt taaaacagcc aagatcatga 130380

agtatgtggc taagtacatg aacgaggcat ctgcagctgg ttatgaaaga atgcagaagt 130440

ctaacgtact ggcagaaaac agactgaaaa ccttcatcga actatctcgt cagaagctgt 130500

tcacccgttg ggctgatgaa ttaacagagt tctattatac actgattcgc acaggtgaga 130560

ctttgcaatt tgtgtgggat gcctctggtg attttgttag cgggtttgtg agaggcctca 130620

actgggtagt tggtgaacta aacaatacca tagtcaagat ccgcatgttt ttccttgaat 130680

gggggattaa atggggattc ataacagcta agacgaagga atattctgaa gagcttaatg 130740

acacctttag ctatgctaaa tgggcagggt ttgctgctgc catcatgctg gtagtgaacg 130800

ccctgacaaa aggttttgtc atggcatcta tgcttgtaaa aaccttcaaa ttccttagtt 130860

ctataagagg cgcagctgga gcagcaggtg caggtgcagc taccacagaa ggatctggtg 130920

gtggtgcggc aggaaaaggg ggaggtggcc tcaagacacc tggtggtaaa gttggcctgc 130980

ttgggaagct tggtattatc ggtacaatat taagcgccgg ggagctattt aatgaccgtt 131040

tcaacccgct gagtgagaga aatcagcaac tggatactat cagcgatatg attcgcaaag 131100

atattatatc tggaggtaac ggatttgttg atcctttgga caccttaccg ggcggtactc 131160

ttccgttgcc aggtggccct ggttttgctt ctcaattaag aactatggaa atgctacctt 131220

tagaaatcag tgatggaaaa atcacgattt caatagatga gggggagttt agcaaaatac 131280

ttgatgcaaa acttgagtgg gagaataaca aagatattaa ctttatgtca acagatcttt 131340

ggtaataaac agcacacagg gggctccggc cccctttatt ttatcggtgt tgtaaaaacg 131400

gctattttat gatacaatat ttagatggta tcggagtgtc ctggagaggt tatgccaagt 131460

aatatagttg atgaaaaaga cctgtacaag cctcaagagg ttaaatctcc aaaggctgaa 131520

aaggcagagc aacaaactga aagcgatgta aagtacacta tgtttgtcag cggtcttaac 131580

tatggtggtc gcaatacgga gatgaagggt aaaaacttta ctaacgatct tgccatcatc 131640

ttcgatcaga ttgagaacta ctctttcaaa cattctgttg ataagactga ttttgcggtg 131700

gaagataaat ctaccctgtc agaccacgct gtaattaaag atggtgtatt ttcttttact 131760

ggtagggtaa atacatcacc acatataatc tatgagcaaa actacattga caggaatact 131820

gactctaaaa accctgcagc atctatgcga ccgggcgctg cactggaggc tttgactgag 131880

atcattaata agcgccaatt ggtttctttg gttaccgagg agagactcct tgagaactac 131940

atcataacca gtctttctgc caacaagtct gttggtgaag gcgctgcgat ggtgtttgat 132000

gtagaactta cagagttccg cacattctac ttgaacaagg ttgttaatgc aactgtatat 132060

agcaatccta agaaagtagg caagacccag caaaaaggtg ctgtaaacga ctgtaagaat 132120

ggaagtaacg taggtaacaa gagggacacg ggggcatttg cagacactgc ctccacaaat 132180

gccgcagaag agtggcagcc atacaaatct atgatgtgga cggatgaaaa cggtgttacc 132240

caatctgctg ctgattggta ttatcaggaa tatggaagac cttctactat agtcccatct 132300

gagcagggaa actacaacaa agttaacgga aaataagggg agcgtgtaat ggaacttaaa 132360

gattggtgca taatcacctt tgtgtgggat attgacggct ttcctgatca aaccatgcgt 132420

gttgttttag acaatgagac gtatgaaatg cgtatgcaat ggaacgagag ggatgagtcc 132480

tggtggttat ctctgggtcc ggtaggtgac agccctttag taaccagaaa agtaacagct 132540

tgtcaagata ttctagacgg actgcattac agagacgatt taccgaaagg tagattgatg 132600

gtgctgtcgt ttaaagacga tcaatattgg ggacgggttg gaagatacaa ccttggagca 132660

agatctgagc tgcaacttgt atacggtacc ccagttgatc tttacaagga acagcaaggg 132720

ttagaataat ggcagagtac agacgtagaa catggcgact gttgtttggt cgtcctgtag 132780

agcatggcgg taaatcaaaa accaatatcc caaaatctgt tgaaaagaaa ggagagagtg 132840

attccggagt ttatgagatt agctcggaca ctggtgctgc caatatagag tttgacatac 132900

aaaaggacaa taccaaagag ccaaacaaag gctatgtgac agtttacaac ctgtctgacg 132960

atacagttaa ctatctagat atgcatcaag ctgatgctct tgcagttatg tttgaggctg 133020

gctatgataa tgagaaccag ttgatattct ctggaactgt agagtttgtt gaggacagat 133080

gggaaggccc gaccagaaaa actaagttca tctttgggga tggcactgag aacatcatta 133140

agtgccaaag cacaagatct tatcagaaag gtacacctct gaactctgtg cttaatgacc 133200

tactgaagga tatgaacttg ccaaaaggca gggttgttgg ttttggaaac cagactttgc 133260

aatactctat ggctttctct ggaaacactg cagagaacct ccgcaggttt gcaaggctga 133320

ccaactcgac gtttagcgtt caggatgggg ctttgtattg gaccaaggtt ggaaaaaggt 133380

tcaaggattc tgtgtttgaa ataagtgcag agtctggaat gcacggatca cctaccccta 133440

aaaacccaga acctgctaaa aaacgaaaag ctaaagctgc tgcgaagggt ggcacaagta 133500

agaaaaagaa aaacccgaga gaggatgttg gtcttactgt aacaacatgc cttaacggag 133560

ctatcctccc agagtccacc atttatctta aaagtaggca atacacaggc ttctataagg 133620

taatcacggt gcaccataag ggcaccatag aaggcggtga ttacactaca gagcttggcc 133680

ttggggaaac aataggtgga gttgttgaca aaactgagga ggtataatgg caataaatag 133740

gaaagatgct gccgtaagat cctttataga aaaaataatg agggactacc acaccagtat 133800

cagggctaga gttgtcagtg tagattacag tatcccctcc gcagcagtgc agccaatagc 133860

tgaaacaacg tttgatgacg gtgatgttga gagataccct cttatctatg atgtgccaat 133920

acaggtgatg tctgcaaaca gtgggaaggc aaggttaaca cttcctatta aggcgggtga 133980

tgttgtagga ttgcagtttt cagagagaaa tgaaaacaat aatgatgatc aacaaactca 134040

cggcctgttt ccagggtggg caataacctc tgttcattca gactctaacg ctctgcagat 134100

cgatcctgat aatgtagagt tatggaatga tagggtttat tttaagctga caccttctgg 134160

tgattttgag cttcaaacac ccggtgggac attacggtgc gacagcagag gggagttttc 134220

tttcaccaac ggagctgcca atttacgagc tgctgtggac ggaaatatta gaatgaatgg 134280

tgccacaata gacccatcgg gcagaatcat aactgctgct ggtgtggatt tggactcatt 134340

ctatgcagaa tataaaggtc atacccactc ttgccctgat ggtgagacaa gtggaccgca 134400

ctaatagagg gcttgcatgg ctacgcttta tagtgatata ttgatggacc cacagacagg 134460

cgatttggcg atagacaaag gtcttaagtt gatagactcg aaccaagtaa gtctgcgcca 134520

gcgtctgtgg atgaggttta atacctggaa agggagctgg tactttgatg agttgtttgg 134580

cttcccttat atggactttc ttagcaagaa ggtcatgaag actgttcttg ataacaagat 134640

tatggaagtg gcaagacaag agccggatgt gctaaacatc ataaacttcc aatctaccat 134700

ggatcgaaga tcgagaacat atcaagcgtt ctttgaggtt gtaaccaagg agaacgagat 134760

tgtaagactt gctttcgtag gtcttgatca gtttacctat ccacagcctg atgctgggac 134820

aacctctctg tgtgatgatg aaggttggat taagtgggct aataaactgt actacctgat 134880

caacttcagg ctgcctagaa ccggggatgc cacatggtgg aaccagtatg ctggtcctga 134940

gatggaaaac ccgatccctg taggctctct gttgactcag gataaagatc tgctgatgac 135000

cgaggcagaa caaaccatta accgcaatac ctggacgctt gctccagatg agcgcgaata 135060

ctctggtgtt attgctacct ctaatgatga ggctattgcc aaccagtctg gtcgtgctat 135120

tgatgttaac taaggagaaa tcatggctgt agcttatcct acaataccaa ttccagatct 135180

tgaagatgca gaaacagtcc tgagtgatga cttcttggtt gtcaatcaga ctgatgggac 135240

aagaaaggct aagattgacg atgttgtcaa cgatctcagc attaccaaaa tagtctactt 135300

cacagagggg ggttatctta agagcaagaa ggactttgct tatgacccag agaccaagcg 135360

ctactacacg tggaacgggg attatcctaa aataatcctt ccagactcta ctgtggaggg 135420

tgctggaggt gtgtctgcaa aagcctggtc tgtttttggg gagcttgctg caacttcttc 135480

tggtagaata gttgattacg gatctattgg tggtcagctt gatatggatc ttgaagttgc 135540

ggacactttt aaagttcgac taacttcaaa taccacaatc gcttttgaaa atcaaacaga 135600

aggccttgaa ggggtggcga gaactataac cgtatgcatt actcagacct ctggcggtaa 135660

caaagtctat tggcctgata atgttaaatg ggcatacgga agagatccta tcctaacatt 135720

tacagctggt gcaacagata ttttcaagtt agaaacctac gacaacgggt taacttggta 135780

cggggcactg attatagcag gagctatcta ag 135812

Claims (10)

1. A strain of high temperature resistant Escherichia coli bacteriophage RDP-EC-20031 is characterized in that the bacteriophage has a polyhedral three-dimensional symmetrical head part, a nucleic acid is wrapped, the diameter is about 70nm, a tail part with the length of about 90nm is arranged, a tail sheath is arranged, the head part and the tail part are connected at the neck part, and the bacteriophage is a rhabdoviridae with uroviridae; the gene has total length of 135812bp, no virulence gene and lysogen gene, and the preservation number of CGMCC No. 21406.

2. The Escherichia coli bacteriophage RDP-EC-20031 of claim 1, wherein the bacteriophage is cultured at 37 ℃ for 1 hour at pH 6-9 with titer still at 10¹¹The pfu/mL is higher than that of the product, so that the product has good acid and alkali resistance; the phage is treated for 30min and 60min at the temperature of 60 ℃, the titer of the phage is only reduced by 1 magnitude order, and the phage has better tolerance to temperature.

3. The coliphage RDP-EC-20031 of claim 1, wherein phage display isThe body RDP-EC-20031 has the potency of 10 at the pH value of 2⁶pfu/mL, at pH 12, still survived the phage, with a broad range of acid-base tolerance of the phage.

4. The coliphage RDP-EC-20031 of claim 1, wherein phage RDP-EC-20031 infects escherichia coli with high efficiency and optimal multiplicity of infection of 0.1.

5. Use of the bacteriophage RDP-EC-20031 according to claim 1 for the preparation of a medicament for the prevention and or treatment of diseases caused by e.

6. Use of the coliphage RDP-EC-20031 according to claim 1 for the preparation of a feed for the prevention and or treatment of diseases caused by escherichia coli.

7. A fungicidal composition for the control of escherichia coli comprising an effective amount of the escherichia coli bacteriophage RDP-EC-20031 of claim 1.

8. The bactericidal composition for the control of escherichia coli according to claim 7, further comprising an antibiotic for the control of escherichia coli.

9. An additive for preventing and/or treating diseases caused by Escherichia coli, which comprises the Escherichia coli bacteriophage RDP-EC-20031 as defined in claim 1 as an active ingredient.

10. A cleaning or disinfecting agent comprising the escherichia coli bacteriophage RDP-EC-20031 according to claim 1.

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