CN114410591B - Acid-resistant and high-temperature-resistant staphylococcus aureus phage and composition, kit and application thereof - Google Patents

Abstract

The invention relates to the technical field of phages, in particular to an acid-resistant and high-temperature-resistant staphylococcus aureus phage, a composition, a kit and application thereof. The staphylococcus aureus phage is staphylococcus aureus phage Sa88P 1%Staphylococcus aureus phage Sa88P 1). The staphylococcus aureus phage has excellent acid resistance and specific killing activity on staphylococcus aureus, and can provide a new phage strain source for industrial production of phage bactericides. The staphylococcus aureus phage also has high temperature resistance, the survival rate of 60-70% in water bath 2h at 65 ℃ and the survival rate of 10 in water bath 2h at 75 DEG C ⁶ ～10 ⁷ The PFU/mL titer can be prepared into various forms of feed additives, drinking water additives, feeds, drinking water, cleaning agents or disinfectants, and can kill staphylococcus aureus in a wider temperature range and a wider pH range.

Description

Acid-resistant and high-temperature-resistant staphylococcus aureus phage and composition, kit and application thereof

Technical Field

The invention relates to the technical field of phages, in particular to an acid-resistant and high-temperature-resistant staphylococcus aureus phage, a composition, a kit and application thereof.

Background

Staphylococcus aureus (Staphylococcus aureus) is an important zoonotic pathogen, widely exists in nature, has wide infection range and strong adaptability, can cause various diseases such as wound infection, endocarditis, osteomyelitis, toxic shock syndrome and the like, and causes the death rate to be different from 11% to 48%.

Phage are viruses that kill specific bacteria by binding to specific sites on the surface of bacterial cells, and are widely available on the earth. Since the use of phage therapy in the treatment of bacterial infections was proposed by the Pasteur laboratory (Paris) in the 30 th century, more and more studies have demonstrated that phages have high antimicrobial activity and specificity, and can control drug-resistant bacteria and prevent damage to the microbial community. Compared with antibiotic treatment, the side effect is extremely low and more rapid and effective, does not inhibit the natural immunity of the organism or cause allergic reaction, and has no infectivity and toxicity to human beings or other mammals.

The invention in China with the prior publication number of CN109082414A discloses a staphylococcus aureus phage with the preservation number of CCTCC NO: M2017394, and has good in-vivo and in-vitro antibacterial effects on drug-resistant staphylococcus aureus. The invention in China with publication number CN 112852754A discloses a staphylococcus aureus phage BP-13A (Staphylococcus aureus phage BP-13A), the preservation number is CCTCC NO: m2015142 and CCTCC NO. M2016535 are strict virulent phages, can effectively kill various staphylococcus aureus such as MRSA, MSSA, BORSA, and have wider host range. The studies in the above documents have focused on drug resistance, cleavage spectrum, etc.

However, the phage also has adverse factors in the sterilization process, and the temperature condition and the pH condition in the sterilization process can also influence the sterilization effect of the phage. With the recent evolution of microorganisms, the viability of some special staphylococcus aureus under severe environments is enhanced, and the conventional staphylococcus aureus phage is very difficult to meet the requirements of clinical treatment.

Disclosure of Invention

In order to solve the problem that the existing staphylococcus aureus bacteriophage has poor sterilization effect under high-temperature and acidic conditions, the invention separates and cultures an acid-resistant and high-temperature-resistant staphylococcus aureus bacteriophage.

The above object of the present invention is achieved by the following technical solutions: an acid-resistant and high-temperature-resistant staphylococcus aureus bacteriophage is staphylococcus aureus bacteriophage Sa88P1 (Staphylococcus aureus phage Sa88P 1), the staphylococcus aureus bacteriophage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has a nucleotide sequence shown as SEQ ID No.1, the preservation number is CCTCC NO: M2020767, and the acid-resistant performance is realized under the condition of pH value of 1-9.

By adopting the technical scheme, the novel staphylococcus aureus phage has a specific genetic sequence, so that the novel staphylococcus aureus phage has excellent acid resistance and specific killing activity on staphylococcus aureus in severe environments. Can provide a new source of staphylococcus aureus phage strain for the industrialized production of phage bactericides

The present invention may be further configured in a preferred example to: the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) has 60-70% survival rate in water bath 2h at 65 ℃ and 10 in water bath 2h at 75 DEG C ⁶ ～10 ⁷ PFU/mL titer.

By adopting the technical scheme, the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has high temperature resistance, can be prepared into various forms of products such as feed additives, drinking water additives, feeds, drinking water, cleaning agents or disinfectants, and can kill staphylococcus aureus in a wider temperature range.

The present invention may be further configured in a preferred example to: the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) is cultivated for 8 hours under the condition of MOI=0.0001, and the titer reaches 10 ¹¹ PFU/mL。

By adopting the technical scheme, the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) can finish large-scale proliferation with a small amount of initial phage, and provides a high-quality phage strain source for industrial phage bactericide production.

The present invention may be further configured in a preferred example to: the concentration of the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) is 10 ² ～10 ³ PFU/mL staphylococcus aureus medium, 10 ⁵ ～10 ⁶ The sterilizing rate of the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) of the PFU/mL to the staphylococcus aureus reaches more than 98 percent.

By adopting the technical scheme, the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has broad-spectrum bactericidal capacity on staphylococcus aureus.

The second object of the invention is to provide a composition containing the acid-resistant and high-temperature-resistant staphylococcus aureus phage.

The second object of the present invention is achieved by the following technical scheme: a composition containing the broad-spectrum staphylococcus aureus phage comprises at least the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1).

The present invention may be further configured in a preferred example to: the composition also contains staphylococcus aureus phage JIP2 (Staphylococcus aureus phage JIP 2), and the preservation number is CCTCC NO: M2016185.

The present invention may be further configured in a preferred example to: the composition includes a chemical disinfectant.

The present invention may be further configured in a preferred example to: the chemical disinfectant is one of didecyl dimethyl ammonium chloride with a final concentration of 0.05% or neoJielin with a final concentration of 0.01%.

By adopting the technical scheme, the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) can be used for preparing a composition for controlling the growth and activity of target bacteria in a high-temperature and acidic environment. The third object of the invention is to provide a kit containing staphylococcus aureus phage, wherein the kit contains the staphylococcus aureus phage or a composition containing the staphylococcus aureus phage.

By adopting the technical scheme, the kit can be used for preparing a kit, can be used for rapidly detecting staphylococcus aureus, comprises, but is not limited to, detecting staphylococcus aureus in a target sample in the form of test paper, kit and the like, or screening target pathogenic bacteria in a clinical sample, and can effectively ensure the detection sensitivity.

The fourth object of the invention is to provide an application of the staphylococcus aureus phage.

The fourth object of the present invention is achieved by the following technical solutions: the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) is used as a biological bactericide and disinfectant under the acidic and high-temperature environment.

By adopting the technical scheme, the microbial agent can be used as a daily bactericide, can specifically kill target bacteria in a high-temperature or acidic environment, and improves the microbial distribution in the environment; can also be used as a biological bactericide for livestock and poultry product cultivation, transportation and preservation, and is used for preventing and treating pathogenic staphylococcus aureus pollution in the livestock and poultry cultivation, transportation and preservation process.

In summary, the present invention includes at least one of the following beneficial technical effects:

1. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has extremely strong acid resistance, can specifically partially or completely inactivate salmonella, and provides phage strain sources for industrial production of phage bactericides;

2. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) has good high temperature resistance, has 60-70 percent of survival rate in water bath 2h at 65 ℃ and 10 in water bath 2h at 75 DEG C ⁷ PFU/mL titer;

3. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has the concentration of 10 ² ～10 ³ PFU/mL staphylococcus aureus medium, 10 ⁵ ～10 ⁶ The sterilizing rate of the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) of the PFU/mL on staphylococcus aureus reaches more than 98%, and the sterilizing capability on staphylococcus aureus is broad-spectrum;

4. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) is a virulent phage separated from nature, and toxicity experiments prove that the staphylococcus aureus phage Sa88P1 is safe and has no side effect; the tested phage does not contain virulence genes or undesirable genes; the invention does not carry out any genetic modification on the tested phage;

5. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) can proliferate well on a non-pathogenic bacterial host; MOI=0.0001, up to a titer of 10 ¹¹ PFU/mL, can ferment and cultivate on a large scale;

6. the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) can be used for preparing a composition and a kit; can be used singly or in combination; the biological bactericide may be prepared by a person skilled in the art based on the description of the present invention and common general knowledge in the art.

7. The product forms of the invention can include, but are not limited to, being applied to the body surface, mouth, rectum, pleura interior, etc. of the host to be controlled in the form of carrier carrying, concentrated injection, or drug infusion, etc.; as one embodiment, the carrier-carrying forms include, but are not limited to, oral aqueous carriers, oral anhydrous carriers, cream formulations, and the like; concentrated injection forms include, but are not limited to, vaccine injection, pleural cavity injection, transvenous injection, and the like; the form of medicament infusion includes, but is not limited to, aerosols, rinse agents, and the like.

Drawings

FIG. 1 is a schematic diagram of the morphological structure of the S.aureus phage Sa88P1 identified by electron microscopy.

Detailed Description

The following examples serve to further illustrate the invention but do not limit the effective scope of the invention in any way.

In the following examples, the strain numbers referred to are all named numbers of the company.

Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) with a preservation number of CCTCC NO: M2020767, a preservation unit of China center for typical culture Collection, a university of Wuchang mountain and Wuhan in Wuhan, hubei province, and a preservation time of 11 months 23 in 2020.

Staphylococcus aureus phage JIP2 (Staphylococcus aureus phage JIP) with a preservation number of CCTCC NO: M201685, a preservation unit of China center for type culture Collection, a university of Wuhan mountain and Wuhan in Wuhan, hubei province, and a preservation time of 2016, 5 months and 26 days.

In the following examples, the formulation of the LB liquid medium was: 10g of tryptone, 5g of yeast extract, 10g of sodium chloride and 1000mL of distilled water, and pH7.0.

The formula of the LB solid medium is as follows: 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, 15g of agar and 1000mL of distilled water, and pH7.0.

The semisolid agar medium comprises the following formula: 10g of tryptone, 5g of yeast extract, 10g of sodium chloride, 7g of agar and 1000mL of distilled water, and pH7.0.

The SM buffer solution formula is as follows: 5.8g of sodium chloride, 2g of magnesium sulfate, 50mL of 1mol/L Tris-HCl, 0.25g of gelatin and 1000mL of distilled water.

EXAMPLE 1 screening and purification of phages

Sample collection and processing

The sample is obtained by separating the sample from water bodies near a colorful logistics market in Jiang Ning area of Nanjing, jiangsu province.

Phage enrichment against target staphylococcus aureus in sample two the above filtrate was mixed with 2-fold LB liquid medium in a 1:1 ratio, inoculated with 100 μl of target staphylococcus aureus strain, and enriched overnight.

(III) phage selection

Centrifuging the enrichment solution, taking supernatant, passing through a 0.22 mu m membrane, uniformly mixing 1mL with 5mL of LB semisolid culture medium containing target staphylococcus aureus, pouring the mixture onto a culture dish containing LB solid culture medium, culturing overnight at 37 ℃ after the semisolid culture medium is solidified, observing whether plaques exist the next day, and recording an experimental result.

(IV) phage purification

The single plaque is picked up in 1mLSM buffer solution, and is oscillated for 15min at 150rpm, and is subjected to gradient dilution, the diluted solution is uniformly mixed with 5mL of LB semisolid culture medium containing target staphylococcus aureus, and the mixture is poured onto a culture dish containing LB solid culture medium, and after the semisolid culture medium is solidified, the culture is carried out at 37 ℃ overnight. This procedure was repeated 3 to 5 times to obtain a phage monoclonal sample designated Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1). The staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has a regular polyhedron head structure and a longer tail, the head diameter is about 70-90 nm, and the tail length is about 230-260 nm. SA88P1 in FIG. 1 has a head length of 80nm and a tail length of 240nm.

(V) phage particle preparation

The staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) is amplified by using host bacteria, after the culture medium is clarified, 8000g is centrifugated for 10min, impurities are removed, solid polyethylene glycol (PEG 8000) is added to a final concentration of 10% w/v, stirring is carried out for dissolution, overnight at 4 ℃,8000g is centrifugated for 20min at 4 ℃, and the precipitate is resuspended in SM buffer. The phage suspension obtained was stored at 4℃until use.

Example 2 determination of optimal multiplicity of infection (MOI) of Staphylococcus aureus by Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) individual colonies of Staphylococcus aureus were picked up and inoculated into a test tube containing 3mL of LB medium, and cultured with shaking at 37℃for 12h at 150rpm to obtain a host bacterial suspension. The bacterial suspension was transferred to l0mL LB medium at a ratio of 1:100 and cultured with shaking at 37℃at 150rpm until the logarithmic phase. Pure culture of staphylococcus aureus phage SA88P1 (prepared in example 1) and host bacteria (MOI = phage number/bacterial number) were added in the ratio of multiplicity of infection, and LB broth was added to make the total volume of each tube the same. Shaking culture was carried out overnight at 150rpm in a shaking table at 37 ℃. After the culture, 5000g was centrifuged for 0min and the supernatant was collected to determine phage titer. Each point was averaged in duplicate replicates to produce the highest phage titer MOI as the optimal multiplicity of infection. Experiments were repeated 3 times.

TABLE 1 titers of Staphylococcus aureus phage Sa88P1 at different infection complex numbers

The results are shown in Table 1, where the Staphylococcus aureus phage SA88P1 titer reached a maximum of 5.2x10 ¹¹ PFU/mL, its MOI is 1:10000. The method has the advantages of small initial input amount and high propagation speed, and can be applied to industrial production.

Example 3 virulence gene or adverse gene deletion assay of staphylococcus aureus phage Sa88P1 this example selects 103 virulence genes identified as originating from pathogenic bacterial in vivo lysogenic phages as shown in table 2, and determines whether they contain the following virulence genes by determining the whole genome of staphylococcus aureus phage Sa88P1 and performing a bioinformatic analysis. The results show that staphylococcus aureus phage Sa88P1 does not contain the following virulence genes or adverse genes, so it is not able to encode proteins that may pose a potential health risk, so staphylococcus aureus phage Sa88P1 does not affect the health of the human or animal body.

TABLE 2 major known virulence genes of lysogenic phages in pathogenic bacteria

Example 4 toxicity experiments

The experimental mice were divided into two groups (phage group, control group) at random, 10 groups (5 animals and females) each, and the phage administration dose was 10 after three days of adaptive breeding of 20 mice each, and half of females and males ¹⁰ PFU/kg of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1), the control group was given an equivalent amount of physiological saline, and the mice were sacrificed after cervical amputation and examined for visceral conditions by 15d continuous administration.

The experimental results show that this dose of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) had no effect on the daily behavior of the mice. No abnormality was seen in the dissected examination viscera. The staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has biological safety and can be applied to feed additives. Of course, staphylococcus aureus phage Sa88P1 can also be used as a health care product or a medicament, and also proved to be safe in the toxicity test.

Example 5: preparation of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and Staphylococcus aureus phage JIP2 (Staphylococcus aureus phage JIP 2), chemical disinfectant composition

Respectively taking the potency of 8x10 ⁹ PFU/mL Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and titer 8x10 ⁹ 1:1 of a 1:1 composition (composition 1) of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and Staphylococcus aureus phage JIP2 (Staphylococcus aureus phage JIP 2) was prepared by uniformly mixing 1:1 volumes of 2 phages in SM solution as stock solution of the PFU/m Staphylococcus aureus phage JIP2 (Staphylococcus aureus phage JIP).

Respectively taking the potency of 8x10 ⁹ PFU/mL Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and didecyl dimethyl ammonium chloride at a final concentration of 0.05% were uniformly mixed at an equal volume of 1:1 to prepare a composition (composition 2).

Respectively taking the potency of 8x10 ⁹ PFU/mL Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and benzalkonium chloride with a final concentration of 0.01% were uniformly mixed in an equal volume of 1:1 to prepare a composition (composition 3).

EXAMPLE 6 thermal stability test of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and compositions thereof

The potency of each L00. Mu.L was 8X10 ⁹ The pure culture solution, composition 1, composition 2 and composition 3 of the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) of PFU/mL are filled in a sterile EP tube and respectively acted for 2h, 24h and 48h in a water bath at 55 ℃ and 65 ℃ and 75 ℃. After the end of the action time, the sample tube was removed and immediately placed in an ice bath for cooling. Phage titers were determined by double-layer plate method after appropriate dilution. Experiments were repeated 3 times and the average was taken.

TABLE 3 titers of Staphylococcus aureus phage Sa88P1 and compositions thereof at different temperatures

The results are shown in Table 3, in the experimental group, staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and its composition were more viable at 55 ℃. Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) has good high temperature resistance, compared with control, the survival rate of 60% -70% in water bath at 65 ℃ for 2h and 10 in water bath at 75 ℃ for 2h ⁶ ～10 ⁷ PFU/mL titer.

Example 7 bactericidal effect of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and its composition in liquid 1 phage counting method: the resulting staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) sample was diluted in a 10-fold ratio, 100 μl of the diluted sample was taken, double-layered dishes were plated as in step (four) of example 1, and the number of plaques was counted from the dishes of the appropriate ratio, one plaque representing one phage monomer.

2. Staphylococcus aureus counting method (potency)

And (3) adopting a mannitol high-salt agar selective culture medium, counting by adopting a dilution coating method, and culturing for 24 hours at 37 ℃, wherein yellow colonies on a flat plate are staphylococcus aureus colonies.

3. The staphylococcus aureus phage Sa88P1 is used for culturing staphylococcus aureus to logarithmic phase in a liquid sterilization effect experiment, the staphylococcus aureus is packaged into different test tubes, the bacterial liquid is diluted by an equal volume of liquid LB culture medium until the final concentration of the staphylococcus aureus is 5000cfu/mL, and the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) with different concentrations is inoculated, so that the concentration of the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) in a sample is 8x10 respectively ² PFU/mL、8x10 ³ PFU/mL、8x10 ⁴ PFU/mL、8x10 ⁵ PFU/mL，8x10 ⁶ PFU/mL. A control group and a blank group (CK) were simultaneously set, and the control group was given a final concentration of 5X10 ³ cfu/mL of Staphylococcus aureus; the blank group was given an equivalent amount of physiological saline. Detection of Staphylococcus aureus after 4hResidual amount of cocci. The results are shown in Table 4.

TABLE 4 sterilizing Effect of Staphylococcus aureus phages Sa88P1 at different concentrations in liquid

The test results of table 4 show that: staphylococcus aureus phage SG4P1 at a concentration of 10 ⁵ ～10 ⁶ When PFU/mL is carried out, the staphylococcus aureus phage Sa88P1 has the best bactericidal effect on staphylococcus aureus in the liquid LB medium, and the killing rate is 98.10 percent and 98.26 percent respectively. The staphylococcus aureus phage Sa88P1 has the potential of being applied to biological bactericides.

4. Sterilization effect experiment of composition containing high-concentration staphylococcus aureus phage Sa88P1 in liquid

Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) composition 1-composition 3, and its preparation method is described in example 5.

Culturing staphylococcus aureus to logarithmic phase, packaging into different test tubes, diluting bacterial liquid with liquid LB culture medium to final concentration of staphylococcus aureus of 5×10 ³ cfu/mL, inoculation with final concentration of 8X10 ⁶ Composition 1 to composition 3 of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) at PFU/mL concentration. A control group and a blank group (CK) were set, and the control group was given a final concentration of 5X10 ³ cfu/mL of Staphylococcus aureus; the blank group was given an equivalent amount of physiological saline. After 4 hours, the residual quantity of staphylococcus aureus is detected, and the detection method is shown in step 3 of the embodiment. The results are shown in Table 5.

TABLE 5 sterilizing effect of compositions containing high concentration of Staphylococcus aureus phage Sa88P1 in liquid

Table 5 test results show that: staphylococcus aureus phage Sa88P1 concentration is 8x10 with high concentration ⁶ When PFU/mL, the killing rate of the composition 1-3 with the composition on staphylococcus aureus in the liquid LB culture medium is 98.70%, 98.30% and 98.38% respectively. The composition of the staphylococcus aureus phage Sa88P1 has good sterilization effect and no antagonism to other composition components. The composition of staphylococcus aureus phage SA88P1 has potential for application as a biological bactericide.

Example 8: acid resistance test 1 of Staphylococcus aureus phage Sa88P1 and its composition the obtained sample of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) was diluted in a 10-fold ratio, 100. Mu.L of the diluted sample was taken, double-layered plates were plated according to the method described in step (IV) of example 1, and the number of plaques was calculated from the plates of the appropriate ratio, one plaque representing one phage monomer.

2. Will have a potency of 8x10 ⁹ The PFU/mL staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) was adjusted to pH1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0, 14.0, respectively, and the titers were determined after 4 hours of treatment. The test results are shown in Table 6.

3. Samples of composition 1, composition 2, and composition were adjusted to pH1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0, 13.0, and 14.0, respectively, and titers were determined after 4 hours of treatment. The test results are shown in Table 6.

TABLE 6 pH stability of Sa88P1 and compositions thereof after various time periods of reaction

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Table 6 shows that: the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and the composition thereof can maintain higher titer between pH1.0 and 9.0. Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and its compositions are extremely resistant to acidic environments and can withstand low pH environments.

Example 9 preparation of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and composition 1 thereof for control of staphylococcus aureus infection during chicken raising composition 1 is described in example 5.

200 experimental chicks were randomly divided into 4 groups (phage Sa88P1 group, composition 1 group, control group and blank group) after 3 days of adaptive feeding, 50 per group, and each treatment experimental group was given a dose of 5x10 ⁸ PFU/kg of test phage and 5X10 ⁸ PFU/kg staphylococcus aureus; administration of 5X10 to the control group ⁸ PFU/kg staphylococcus aureus; the blank group was given an equivalent amount of physiological saline, followed by 15d. Daily growth and survival rate of chicks were counted, and the results are shown in Table 7.

TABLE 7 influence of Staphylococcus aureus phage Sa88P1 and composition 1 thereof on chick survival

Table 7 the results show that: the control group died all the chicks on day 9; the survival rate of the blank group is 92%; the survival rate of the single staphylococcus aureus phage SA88P1 group chicks is 76%; the survival rate of the chicks of the composition 1 is 96%, and the survival rate of the chicks of other compositions is 84%, which shows that the staphylococcus aureus phage SA88P1 can be used in cooperation with other staphylococcus aureus phages, and the effect is better. The experiment also shows that the staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and the composition 1 thereof can be used as a biological bactericide or a feed additive, and can effectively prevent and treat staphylococcus aureus infection in the chicken raising process.

Example 10 control of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and compositions thereof on staphylococcus aureus contamination during chicken sample preservation 1. Preparation method of compositions 1 to 3: the procedure is as described in example 5.

2. The autoclaved chicken breast was cut into 1cm square squares of 90 pieces, divided into 6 groups (single phage SA88P1 group, composition 1-composition 3, control group and blank group), 15 pieces each, and placed in sterile dishes. 5X10 of each block of the experimental group was inoculated ⁸ PFU/kg staphylococcus aureus and 5x10 dose ⁸ PFU/kg of test phage; control group vaccinated 5X10 per block ⁸ PFU/kg Staphylococcus aureus and an equal amount of sterile water; the blank group was given an equivalent amount of sterile physiological saline. Each treatment was placed in an incubator at 37℃for cultivation. Every 2 hours, a piece of chicken is taken and placed in 10mL of sterile water, the mixture is fully vibrated, the staphylococcus aureus content of the liquid is measured, and the experiment is repeated for 3 times. The results are shown in Table 8.

TABLE 8 prevention and treatment results of staphylococcus aureus phage Sa88P1 and composition thereof on staphylococcus aureus pollution in chicken sample preservation process

Table 8 the results show that: after 8 hours, a large amount of staphylococcus aureus grows on the surface of chicken of the control group; in each experimental group, staphylococcus aureus on the surface of chicken is always controlled at an extremely low level due to the addition of staphylococcus aureus phage Sa88P1 and compositions 1-3 thereof. The staphylococcus aureus phage Sa88P1 and the compositions 1-3 thereof can be used as biological bactericides and disinfectants to effectively kill staphylococcus aureus on the surface of chicken and prevent and treat pollution of staphylococcus aureus in the process of preserving chicken.

EXAMPLE 11 preparation and use of kit of Staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa88P 1) and its composition kit containing 5-10 mL of the kit with a titer of 8X10 ⁹ PFU/mL of a composition of Staphylococcus aureus phage SA88P1 (Staphylococcus aureus phage Sa88P 1) or Staphylococcus aureus phage SA88P1 (Staphylococcus aureus phage SA88P 1), 1L of LB semisolid medium, 1L of LB solid medium.

The using method of the kit comprises the following steps: taking the potency to be 8 multiplied by 10 ⁹ The split spectrum of the test phage was determined by double-layer plate spot method using PFU/mL of a liquid of a bacteriophage of Staphylococcus aureus Sa88P1 (Staphylococcus aureus phage Sa P1) or a liquid of a composition of Staphylococcus aureus Sa88P1 (Staphylococcus aureus phage Sa P1). And selecting single bacterial colony to be detected, inoculating the single bacterial colony to be detected into a target liquid culture medium, and carrying out shake culture under the condition of combining the growth characteristics of the strain to be detected at the target temperature to prepare bacterial liquid of the strain to be detected. mu.L of bacterial suspension of the strain to be detected is mixed with 5mL of LB semisolid culture medium and paved on an LB solid plate, and 10 mu.L of staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) liquid or staphylococcus aureus phage Sa88P1 (Staphylococcus aureus phage Sa P1) composition is dripped on the plate. And (3) culturing at a target temperature according to the growth characteristics of the strain to be detected after natural air drying, and observing the result.

The embodiments of the present invention are all preferred embodiments of the present invention, and are not intended to limit the scope of the present invention in this way, therefore: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.

Sequence listing

<110> Fei Ji Leke (Nanjing) Biotech Co., ltd

<120> an acid-resistant and high-temperature-resistant staphylococcus aureus bacteriophage, and composition, kit and application thereof

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aatacagcat catatgacat tgattgtcct gtttgcataa gtgctgtagg gaacaattga 1080

acatgacctg ctaaatgctc tactttccac cagttagcag ggtatttata tataggtcta 1140

ccattaaatt gtagctgtaa gttctctacc tgtaatatag gttttctgta agcatgtaca 1200

aacatataac tattaaactc tgtctcatga taatctctca tctcatgttg agtatcaggt 1260

agtatagaaa tatctagcgc tctctcagct ttacctactg ctctttctaa tatatggtta 1320

taaaactcat cacccatggg ctgtcctgta tcagggtttt gtactacaat accaaacata 1380

taagctttaa ctgcatcagg tgaccatcca taatcagcta atgttaagtt atctatctca 1440

cttacgtcta tatgtttagg attaccacta ggatgataag gatattcata gtttaatgat 1500

ttttcataag ggtctaagtc ccctccaaac attgagttaa ccatttaatt caccctttac 1560

ttattttcat cttttttggc tgttgtttta cgtgctgtag attttctagt agtacttgta 1620

gtttttctag tagaggcttt cttttcctct tctttaggtt cttcttcttt actagtagaa 1680

gctgattgtt ttctagtagt tttcttttct tcttcaaatt caaaacctgg aacatttgca 1740

aactcttttt cttgttcctt agttaatcct ttaactactc catctttgtc aatatctact 1800

gtaccataaa tggtagccat ttttgtattt agtaatttct tatagtataa catatttatt 1860

gagtcccttc aagtttattt taataaaaaa gggataaacc ctaagtttat ccctattctc 1920

ttgtattcag tttttcttat tctattaaac tgcgatataa cgaacgtttt taatacgagc 1980

ccatttttta ggagcacgta atgctaatgc accataccat aatactgcaa atgtaataga 2040

agcattaatt tgagctaatg gtaatttcat cattggaagt aattcgaata agtgaactac 2100

ttgtggtgac atttcaccaa cgaatacgtc tgctgtttca ggcaatgttt cgttcttatc 2160

tacgaatacg attgttccat cttcttgtgc atctttaact ggtacacgtt tgattaggaa 2220

gtacatacct gtttctttac cttgacggta gatagaaaca aattgtggtt gttgttggta 2280

catagcgtta acactgattg aaagtttaac accatcgtca acgttagata ctgtagctgt 2340

tacttcttca gaaggagctg attgagcgtc atctgagtta actactactt tatatgataa 2400

tcctgcacgg tcttcttcat cttcaaaagc acctttttgc ttagtttcaa ctgtagctgt 2460

aactttagca ggttgaggag cgtttggtaa tggttgtaat gattcatcta agattaattc 2520

attttccatt actgtagaac catgtaattt aatgaatcca cgagatgagt agaaaccatt 2580

tacactgtaa ccagtgttaa cgttaccgct gttgtcttgc attaattgca tttgacgacc 2640

taagattgag ttaacgaagt ctgcgtgtac accaataggc atgtaagcat ctgtagctgt 2700

accgaaacct ttaccgatac gtacagccgc ctcgtttaag tgtttctcag ttaattggtt 2760

acctttagcg ttaattacgt tatttttgtc aatcaattta gctaaaccat caaattctaa 2820

accttcacct tcaacttcag aagttaatga agcatcaccg tagaatgaag cccactcaat 2880

tgtttttgca acaactgcga tagcatcttc tgtaaggatt tgtgatgggt cagcaatgtt 2940

atttactaaa cctgatgcaa ttgacatgtt tttagtatca gaaacgtatt tcattgatac 3000

ggttttttga cggatatttg ggtcagatac tggtgctact ccgatttctt taacgaaacg 3060

agagtgacct acgttaccgt gacgtaaata ttggtcgtat tttactactg tagattgagc 3120

aggacggcgt gagatatcac gatagaagat taaatcttca ttagtccatg ttaacattgt 3180

gatttggtca tctaaaattt ctctacgtaa agctcctgcg tcaatttgtg tatcaggagt 3240

gattccataa ccagtttgga atgactttac tacgtcttct tggaattggt cagcgtactt 3300

ttgttgaacg tctgacaggt tcttttcgat agtcatataa atttatcacc tttcatttgt 3360

gttcttttta ttttatgtag ggtacagtaa aatgtaccct atttatattc tttagttaca 3420

ccgtataata taacacaact ttgtgcatta attaaatttg tgcaaagtct tttacagttt 3480

taatatcttt ctctgatgca ttagtagggt cagtgttaat gttcaagtaa gattgataag 3540

ctgattgtaa gtcatgttta gaagctctag ggtctttagc ttgtgcttta taagaatcca 3600

tgaacttagt tctttcttct tgacttaatc ctttaaatgt gttatcctct tgtacttctt 3660

cagcatcttc tgattttgct tcaccagttt cagcttgctc ttcagtgtct actgatttag 3720

aaacataacc ttctactttt tcttgctccc catttgtaga tgttactgat ttttctacag 3780

cttcttcttc atcagtgtct attgatttag aaacatcttc agattggatt tcagaaattt 3840

ttgctgataa ctcattaata gatttactaa cttcacttaa atcgtcttta gtagcaaagt 3900

tttctttctc cttatttaag ttttcaaaag atgttaggat agatttaaat actgttgtga 3960

tatcctcatc agaaattgat ttagaagttt tttccttctt atcttctttc ttatctttat 4020

catcgtcagt atctttgtct tcatcatcag attcagaatc ttctttttta tcttttttat 4080

ttttacgttt ctcgttgtct ttattttctg ttttagtatc tttttggtct acagggtctt 4140

ttgattcttt agctgatttt tcaacttcct catcttcagt gtcctctgtt acctcttcag 4200

attcaacttt ttcaccttgc tcttgtgctt ctttagcatc ttcctcagta accttttcag 4260

gttctttagc cggttcttca gttgtttctg ctacttgttc ttcggtagat tctactgtat 4320

tcttttcatc atcttctaca gatttcgaaa ctgcctcttg agcatttaac ttatcatact 4380

cttctaaaat attttgcatt tctttactca tagaatgtat tctccttacc tattctagtt 4440

tttgtttgtt tatatccatt actgctagtt ctgcatcttt acgagataaa cctttagcta 4500

gttgtaacgt aaggactgat tcctcatatc ccatactatt agatttactc aaatcttcaa 4560

caacgccatt ccatacatca ttaaactctt ttaaatcttt aatcttagtg acgtaagcta 4620

aatttgtaat gctagatgct atttcttctt tccttaaagc tcctgcatca acttgagtgt 4680

caggtgatgt accgtgacca gttaaaaatg atttcataaa gctttcccat gttgcttcag 4740

gattagcagg atttttaact aatgcaactc ctgttatcat aacttcatca ataactctat 4800

tgtcatttat gttacgtttt ttaactgcac cttcaataga aaaacctaaa cgtcttccac 4860

tacctgattt ttctaatttc tcagctaaat caagcatctt aacaacattt tcatcattct 4920

tccatagctt tgcttcaata aataaacctt tttctatatc cacatagcaa ttctctgtag 4980

gtatacctac aactttatca ctttgatgct cataattaat gtacccttgt gacttaaaat 5040

actctatatc tattccttta ggattaacta tatcattctg taaatccata cttggagtac 5100

tagcccatcc tgaaactata gaatactcat tagtatcaga agctgttgat acagatttct 5160

tcaaatccat aggtacaaaa gcattaaatt ttatttcttc caaaggtgtc tacacctcca 5220

cttgttattt tatttacttt tacgttaata atataacaat aataacttgt aagtgaaata 5280

ttaatatttt ttatgtatta atatcaatta catatagtat atcatagtta ttacctttat 5340

caaaattaaa ttacaaaaaa ataaacaaag cctagattta ttctaggctt attaattaat 5400

gtttaaagtc agaagatttt tctccttttc tcccttgacc tttattagat gtttgagttc 5460

ggtaaacatt atcgtcacct tttatttgcg catctgttcc tatttcttta tcagcattac 5520

tagaatctgt tgattgactt tcttctgaat cgtcattatc tccttctagt aaactcatca 5580

tcatttgtaa acgttctttc tgtttaccat cattatattg tttatcttgt tgtaattgtg 5640

ctgttccttg taagaatgaa gcatctaaga taatgtctcc accttcaata ggtttcttac 5700

cttgttcttc tctagcctca ttgactgttt taaatatttg agtttctagt ttaagaatat 5760

taagtttgtc cgtagcactc ttagtatctc cacctacgaa ttggaatgta tatttatctc 5820

catattctga tataatatgt ctattaacta aatcttcaat aaatctaagt aaaggttgta 5880

aacctttatt ttgagattgt tgttgttttt tacctgggtc agcctcattt aaagtagaac 5940

cacctttaga acctgtagct cctcctctat tagggaaacc aatttctgca gggtcaatac 6000

catacaaagc agatataata ttaataagat aatttaacca tttctcaaat tgcatatcat 6060

tagcagtcgg tgtcatatta acaaatttaa tatcgtctgc cattaccact ggaatttgcc 6120

atgaaccatt gatacccgat aaacttgatt tccattcacg cttgaagttc tctaatgcat 6180

gttgtgattg ttgttggtct gaacgtattt gtaaaatacc tcttgtagta ccaccatgtg 6240

agaaaaatct atcgttaaat gattcagtat tattataagc aataaattct ttcatagcta 6300

tctctacttc tgataatccg taccctgaag aagataattc agttctagga tttcttatac 6360

ccatagctaa ttctctagaa gtaaaactag ctactactct tttatctact acttgaacaa 6420

acctcttacc acccttaata attttacctt ttttatctgt tgcataaaaa atagtagaag 6480

ggtctactgc tatgaatttt tctaatctag ttttattgtt cttattaaat actttttcaa 6540

agttaacttg gtcatatata taagtatctc taacaatttt cttacagaaa gtttgaaacg 6600

aatctctatc tacatcttta tctttacctg tattaacaat aaaatcttct atacgtttca 6660

tttcttcttt ttcttttcta cctggttctg catctaggtc tcttaatctt acctcaaaac 6720

ctaaaccttt ctctgagtat cttgcaggtt gacaatacat agctacttga tttgaacgtg 6780

taagtatgat agcattaaga ataggattat taccaaactt tttcaaaaca tcgtgtaaat 6840

tatgttcatt cttcatgtaa cttctcttat ctctaaattc aggattagta tccatcattt 6900

ctataaatgg ctctgcataa gcctgttgtt gtccgtataa agacttagtt aagtcttggt 6960

actctttatt gtcttgttct atttttttaa tgttagcttg taatccttca tcaataggaa 7020

cttgagcaat ggtactatta ttaccatagt ccttacctaa tctgaattgc ttaaataaat 7080

ctgccaatta ctagtcacct ctactccctc ttactgtagt taatacaaca gtattattat 7140

aagttacatc tctaatatgt cgagggatat ttattttaac ctcgaacggt tcttcaatgt 7200

aataaaacat ttttttactt tccataacaa tatcataatc ttctaatagt atattattat 7260

ctttatctac taatattact ttattgtctt ttgttgcttc tagaatcatg tattttactt 7320

tgtttaaagc aataaaaaca gctacgtgtt ttccacctat ctgtctataa taatctacaa 7380

tctctatcca attatcttgc atcccttgac ctttgatgaa atcttttgct tcatcccatt 7440

tcttatcttt agagaatatc aacctattca ccgtcttttt tattttttat ctttaaagtt 7500

actcttttta ataattttca tcatcttgta ccaaattcct ccaagtattg cccctataaa 7560

acttatagta aatataatta tagggtcatt gaatccaata taatgttcag tattttctat 7620

ggaatctatt acatcattct taataagtac agatgttgtt tgaaacatag ttaaaactat 7680

gtatgtacct attcctatta tagcataaat tacatacaaa acaaaaacgt ttttactgga 7740

gaacttactg tgaacaaaaa tcattaaagt taaaagactt aaacaggtaa atactactga 7800

tagtgacgtt ataacattca ttcctatcaa agttttcacc accgtaatgt tcaatttttt 7860

tatatttatc tcttatttaa tatagcaaac aaaaaagagg agtaattact cctcttcagt 7920

tttatcctca ttatctgtat tattatcgtc cggttgttct gtaggttgtt tatcctctgt 7980

tgtatcttcg tttttatcct cttcgtccac cttatcgtcc ggctcagaag gtgtttcagg 8040

ttcggaaggc tcagtttccc atcctccatt ttcatcagga ttatctgatg gttcggatgg 8100

tttttcttca ttaccattac cctcattagg tttttcatta ggttgttcag gctcagaagg 8160

tgtagggtca ggttcaggag ttacctctcc ttcattgtct cctgtattac cattatctga 8220

tggctcttct cctgggtcag gtttttcttc ccaatctcct cctcctgtat taccgttatc 8280

tgaaggttct tccgttggtg ggggttcaac attacctcca ccacctgagc ctctacctcc 8340

atcttcatct gtatatcctg gaggtggagg gtttggatta tccgtattat ctatatttcc 8400

tgaattattg tctacaggat tttcaggtaa tataggaact gacggattat caataggagg 8460

taatatatct gaaggtgtgg ttggttcaac tggatttgtt ataataggat tatcctttag 8520

tacattatca gtagaagtat ttgtatcttc tactatacta tctactgtat ttggataaac 8580

attactatta atgataccta aactatctat accgggtgaa tcagttgata agaacgggga 8640

acctaataca ctagtaataa tagaacctac tgtttgtttt tcataaccac tgtttgtagg 8700

gacagttatc gtaccaattc catcttcact aataagtatt tcaggtttat ctaccttatc 8760

actaaactca atattatttt ttactaatat taagtcatca tcaccagtta catcatatat 8820

tttctctcta gtttctttat cttccttttg tctttcttta tttgacttct tattatagtc 8880

tttataattt gtttctttct ttactttttt aagttcttct tcagctttat ttaatctttt 8940

tacgtatttc tttttagtct ctccatcatt atcatctgct tttttataaa tctcaactaa 9000

tctctgatat ctatctttat cttctttagc atcttggtat ttattaaaaa atatggtcat 9060

aatgactaca aatattgcta tcataaccgt tattatggtt atgattatct cattgccttg 9120

tggtttcttt ttcaattatg atacctcatt ttatatagtc tcataattct tcccttttat 9180

aagttattaa gtttctcttc ttcttctttt ctaattcttt ctttttcttc aaagtctttt 9240

ctaagcataa acgaaatact tccttggaac tcgttacctg aaagaatatt acttaatgct 9300

gtatctataa tagctacatc acctagttca attgccttac ctgattctac atcttttctg 9360

aattgcttgt tagcttcaaa aatatcaatg cgttttgaaa aagcaagttt atacatcttg 9420

tcatgtaaat ctcctgttaa agggtcattt agtctacttt gaaagaccat tagagaacca 9480

tctaaacgtt gcgtttcatc catagggtct tgtctaaata cattgtaaga cttgtataat 9540

ctatttacca tttcacataa taaactacta aattctttaa tactatcctc tttctcctct 9600

ttactaagtg gagttattga ttcttctaat gttaatttat tttcttctaa agtcatttat 9660

ttcttccttc cttttttatc tttccttcaa tatcttcctg taatattttt aataattcag 9720

ttgtgttatc tattctattc atattagact ttacataaat tgtttttaat ataatataga 9780

gaataaatac aatagaatag atgactacag tgctaaataa aatcactata atcacctaag 9840

aattgtttta catctttttt tcttaagatg tatccatctt tattaggttt aaatcctaaa 9900

gtttttaaat actctatagc ttcaggcaat tctatgtcat acacaactgc atttttattt 9960

tccttaatac ttaaacgtaa tagttccata atagctctag cccctacacc atctgaatcg 10020

ggtgaacttg cataagattc taatattata tcattttcag ttcttttaaa agatgtaatt 10080

cctaatgtat ccccattatc atcaacaaac tctttgtaca atgatgagtt tgtactaaca 10140

tgtgatacat cgacaccttt tttacttaaa ctagataatg aatttgctac ctttttaggg 10200

tttacctttc cttcttcacc attagaaata gctcttgctc ctactgcact tctactaact 10260

tcttctttgc cttcttgttt ttctttacta tcattttctt tattaccgtc ttcgtaaact 10320

gtcatttcca tttctttacc attacgaata acagtacgtt taaccttatt tagcccacca 10380

agatttttat ctccaccttt atggactatt tcaggttctc tattagcata taaaaaacaa 10440

tcaaagaaat tatcgaatcc tagagattta taaacatgtt catatttaac atctaaggta 10500

tcactatttt tagaaacata atctaaagat aattcctcac ctaataacaa attcaataca 10560

cttttattaa tttcttcatt actaattgta ccataaaata atttatctgt caagtctatt 10620

tcacctctat tcatcaaagt agaattgttt atttccttct tggttgtaat cagtagaaac 10680

aaatgtagaa ccaaagcttg taccatttcc ttctttaaga agttctttta ttcttgttaa 10740

tcctatatag gcataaacac ttgcttgtgc ataatggtcg tcacctttac gtttaataac 10800

ttgatacatt tctccagttt tttcatcttc ttcatccata ataacaacat tttgccaatg 10860

ttttaagaaa gtttttaaat catcatctac tgtactataa acacttatat cctttgtctt 10920

aagtgcttgt atatatcttt tattctgcat taatttatct actgtaaccc tattattgtt 10980

ctcattaaat tcaggtctca actgtcctgt agacttagga gaagatttat acgtacatcc 11040

aaatacttta tcttttccaa aatgattaat gagttttaga acattgtttc ctgaatctcc 11100

gttatctgca attataatat cagggtcgta cttagatatt tcccaaatga ttttttctaa 11160

atctgcttca actaaatcag gtcttgtcat ctttttaaca gagaataatc gtattaagtc 11220

taccttacca ttaggtaaca taccatgaac agttatccaa tgagtatttc cccaatctat 11280

accaatagct atatgagaat atctatctcg tttcattaat tgtgtttctg caataggtga 11340

tttgttacca taaacgtctt cttcattaac tctcagttta acatcttcaa atggataacc 11400

caaaatataa ttgtaaaacg cttgcttaga ttctgtattc atttcttttt cttttaattc 11460

atcagcagaa atccatacag cgttcatttg tgttattagg tatcctcgta cccctttatt 11520

accttttgta cgctcaggat acttacaatg ccactcacca ttataccatc tatctagtgg 11580

tttaccgcat ttttgacaaa caaattggta actaccattc tgtactgttt tagcctgttc 11640

atctacacct tcagggttaa cacataacat attcccactt tcttcaagat tatcagggtt 11700

gtaatcatta taactcattt catttaaata atcacaatgt tgacatctat gaccataata 11760

ccattggtca gattgttgat ataatttgtg tatacccata ccgggtacag atggtgtgct 11820

ccatcttctc acaatcttaa aaggtgatga agacattgac tctaatgcag atgactctgc 11880

taataagttt accctatcat actcatctaa ggataagtag tcaatatcca caccctctac 11940

agtacttgct ttagaacttg ttctaaagaa taaactagag tttcttatct ttttaaaacc 12000

taaagagtct ttatcccaat caacaatatc tctaaaatat tctttttcta atacagggtt 12060

caaacgagac tgaacaaatt ttttcatttg ttcatttgta gggaatgtgt ataaacactt 12120

tgcattggca taactatgca tatctgcaaa atgaaccatt tccattacac ccatctcact 12180

aagacctaac tgacgtgatt ttattacagc cttattagga tgagtatcgt ttacaatcct 12240

agtttgccat ggtctatgag cctgtgcttt acttctatct ctattaggaa tgctgaaagt 12300

tatagggtgt cctcttagtg tgtgatactt aagcatatag gtagaagggt ttaacatatt 12360

gattatatgg tctatctgtt ctcttgttat tttttcagtt tgaaatgttt cttgtgctat 12420

cttaattagt tcttttccat ccatttatat ctttctcctt agaatgtttc tgaattttcc 12480

tcattcatta ctttttcttt ttcagaaatc attgctgtaa tatcttctgc tgtcatttct 12540

tgtatcttct gtaaatcaac tgtagattct ccattactat cttctgttgt aatctcgtca 12600

aataattctt gctgaggtct agatagctga ggtattgctc caccttcaga agtttcatta 12660

accatatttt gcatttgaga gaatatcacg aatagtttat ataaatcatt agggtcttta 12720

acttctattt ctttattatc tactttttta ttgaattcat acattaattt acctatacca 12780

ttcataaatc catcagctat atattttctt gtatcctctt cttctaactt tttcttgtta 12840

attatatctc taatattttc tgcactcaat ccatttcacc tctttttctt aaattcttat 12900

aacaattgtt gatatctgcg caaatatcaa aataaatata cttagaatac tgtaaatgaa 12960

aatgattaac ttttgataat gcttttcctt tagtgtaatt catacctgat aactgctgac 13020

cgcataggct acaaattcta ctagggctag ctattctctt atctcctttt tttactaaag 13080

aaacagatgt agcaccatcc ttaagctctt tcctttgttc tttaatttct ttctttgttt 13140

tcatcgtaat cttccttttt actatcttcc tctattcttc tttgtgcttt agccctaact 13200

tttatctctt ctgctgttat accgtagtta ctttctatga ttttcttgat tattcttatt 13260

tctttatcat ttatctctac ttttttaaga acatattcag gtagggggtc ttctttcttc 13320

agtaagaact tatctgttat agtaagtgcg acattagaaa caataccacc aaatataaag 13380

tatgtcataa ctacactcgg tcttattgaa taaataaggg acaaggaatt aataacagac 13440

aaaattagta gtaagaatat aatgataacc agtgttttga atatgaatat agctatgtta 13500

ttattcaacc actttaacct cctattgtct ttttttatta tattattaat ctaacaattg 13560

acttttatga ctggctttta atgttatatt agtggtgtaa tctaaaagtt ctataaataa 13620

taggtcaaaa agaaataggt gtttaacaat gattatcgta tctttttttc tgtctatacc 13680

tcttttttgt ttctattatt ttatacttag tggtgttaca acgtctttag gttattctat 13740

ctataaagaa gataaaagta aggtaactct taatatagta tatctatcag ctatctatgc 13800

tttatctgtt tttagtattt ggcttatttt ctcagaaaaa ataattatta tcattattct 13860

taatataatt ataataactg cctttatcat aactattata ccacaaaata agtaaattta 13920

aaaattaata gtaaggaggt taccactttg actaaatata aggatatcct taagttagag 13980

tttaaagatg cactagccca ttttaaaaga gacagacgct atttccacgt ctatagaata 14040

gacagagtat taataaatgg ctcaattata tacttcgact attattacct tccttctgat 14100

gaccctaata ttgtaataaa agaattagac ctccaaagtt ttggtaagct taggtttgaa 14160

attgatacaa aaacatctta cggaaaagtc gttacggata attatatgga gattatcaat 14220

gatttcttag aaaattatga tatccattct gaatctgaaa cggttagacc ttagaggtga 14280

ttctagttgg ataaggagat aaacaacctt gtttctcaag tagaaactat taagtctaag 14340

atacaagaag gtaattatat agatagagga actttcaaag atttagaagt tgaggtagct 14400

gaactaagaa aaatgatagt tagtatagat aaagatgtag ctgtcaatag tgaaaaacaa 14460

tcagcaattt acgtccaatt agaaagactt gatgaaaaga tttcagaact tgctgaaagt 14520

acaaaaacta aagatactag gaagaaggat actactgaga aggttctttt actcgttcta 14580

ggtgctatac tatcctttgt ctttaacaaa tttgcatagg agagattgta gatgtcaaaa 14640

catattgaaa taacaatgtt aagtggtgct aaatattttt tagtatcatc tgacgaaaag 14700

ggttacacta gaaaagatat agactatatg attagtggtt ctgattcagc atcaataaaa 14760

gttaatacaa ctgaagatgc tagttctagt ttaatttata ttaacccaag aatgattgaa 14820

tcatttaaaa tagtttttta aattaacaag tgactttaag tagtcacttt tttatttgtc 14880

tttttgttga ctctctaacc ttattaatta taattatata tttagatagt aattaataat 14940

aattaataag tatatataac tataatagga gagttcctta ttttctaaat acgtactttt 15000

ttgtctgtaa gtactgatac taaaggattt tacttctata ttgagtagtt ttaccaagta 15060

tcagattata tttaattcaa ttttcccaac cttattaatt ataattatat atttaatatt 15120

attaattact tatatcagaa tataattata tataatagga gtactcctta ttttctaaat 15180

acgtactttt tctcttgtaa cccttggtat taaaggattc ttcaaaataa tttaaattat 15240

ttttagaaaa ctgttgactt atttatcaat atagtatata gttaaattat caaataaaac 15300

aagaaaagga attgattaat tatgaaaaga caaaaaatgt tttactcaag tttaatatgt 15360

aaagaatgtg gtaatgtatt caaagtacca agaaaaagag caaataaaag agaagaaggt 15420

catatcaaag atatatactg tatcaaatgt tgtaaaacta caaaacatat agaagataat 15480

cgtagtgaag cagaaagaag atgggatgct attcaggaag aactaacaaa agataactaa 15540

tattttaaaa aaagtattaa attaacttga caaaggtatc agtatatgct ataatgtaat 15600

taacaataaa acaaatacct tacacatcac tgttttttct tacattgttt tccagtttaa 15660

acaataacaa tgtgtatcat ccttataatt ttaattggaa gtaagaattt tatcttactt 15720

ctataatttt tttttaaaat taacttgaca agtactaaaa attatgttat aattaatata 15780

taataataca actaaatatc tgagcatagt gactgaataa tgatttggta gtcataaggt 15840

acaagataat caaacgaatc aaaacctatc ggaaatatat cggtaggatg ggtcgtcatt 15900

agtattgtta aatacgattg gtatacttgg tacttactta taaggtctgt agagtatgat 15960

aacggctagg gtttaatttc tttctagggt cttagcggaa tcctagaaag atacgctgta 16020

acgactaact gtagttggag cacagaaatg tgttagataa tagaggttat tagtatgagt 16080

agcataaatc tacaggttca aaagaagcta tatttgataa aaataatttg gaaatcaaat 16140

aaatttactt ttcttcgacc atgtgaaagt tattaactaa tatgttaata tcacattatt 16200

taataaactt actatcgtta tacggtttgt aagtcccttt taaattgcgt gagatggact 16260

taaggtagct ccttaataga gtcaatcgta gtgattcact taataacagg tcttataggt 16320

aggttcctcg tgagacgggt tatgcttgga tatttagttg cattatatca gaatgtgact 16380

ataaatattt tcctctttat agtttattat taacaattat cagtttcctc ctttgattga 16440

taattaaaaa agaatctaca aggagtagac tttcactctt tcatgtattt attgttcaca 16500

ctcctttatg aaccaataac ttactccttg tagattatac atacttccta tataagtacc 16560

aattaccacg ttttgttcat ttcacctcct ttcacttaat atatagttgt tatgagtggt 16620

aataatcgtt gcttttagta atccttatat aggaaatatg tgtttaaata agtaattaat 16680

ttactcgacc gaattaatta cttatttaaa cacatagaaa ggtcgagata aagaatgaaa 16740

aacatagaac aatggaaaga catacccgga tatgaaggaa tataccaggt aagtaattta 16800

ggtaatgtaa agagttttaa aggtaaagaa aaaatactaa aacaatataa aaataaagat 16860

aatagatata ttgttacatt gagaaataat aacaataaca aagtatatac tacttattat 16920

ttagttgcca tttctttcat aggtgaaaaa cctgaagggt acgaaatatg tcacattgat 16980

ggtaactcag aaaataacga gttatcaaat ttaaaatatg atacgtgctc agaaaaccac 17040

ttagatgtat atagatacgg tggtaaaaac aataaaggta aattagaaat aaaagatgta 17100

ttaaatataa gaaaactata cgatactgga aataaaacag tatcagaaat agctaaactt 17160

tataatataa ataaaagtag tattagtaga atagtcaata gaaagaattt tccatggtta 17220

ttggatgatg gttctataaa agagagtaaa actcaaatta aatattaaat attttccttc 17280

ttatgtacca cataatgctt cgagcataag agaaaacctg ctatacatta aggtaaggtt 17340

agggtatagc atttattttg gctcattggt gtaactggtt aacacactgc cctgtcacgg 17400

cagagagtac gagttcgagt ctcgtatgag tcgttacaag gtttcttagc tcagatggta 17460

gagcactaga ttgaagctct aggtgtcatt ggttcaaatc caatagaaac cataataaac 17520

acccttagta taattagtag tacaagggtc tccaaaaccc ttagtctttg tgcaaatcaa 17580

agagggtgtg ttacataaat gaaacttgag aattcacatg tgcggttgac gaatcggttt 17640

gctgtgtcct cgaatgaggg tagagtttca ttgagtatat agcgcaagtg tttgttccta 17700

ggatacgata ctagctatat actttacata tttaaaagga gagatacaaa tggcatcagc 17760

aaagcaatta tattatacgg aaagtttagt tggtaaggca attattaata ataaagtatc 17820

taataaagaa gaagtttggg ataaattaga gttattacct gaaactaaat tagaagattt 17880

agataacaaa caaatgtctg aagttatcaa aaaactaaat caaattaatg agtaagtgtt 17940

aaattaatat agataaacat gaccgaccta ctgttatatt attgttagaa ataaatataa 18000

tagaaaggtc ggttttttaa tggctaatga aactaaacaa cctaaagttg ttggaggaat 18060

aaactttagc acaagaacta agagtaaaac attttgggta gcaattatat cagcagtagc 18120

agtatttgct aatcaaatta caggtgcttt tggtttagac tactcagctc aaattgagca 18180

aggtgtaaat atcataggtt ctatactaac attattagca ggtttaggta ttattgttga 18240

taataatact aaaggtctta aagatagtga tattgttcaa acagattata taaaacctcg 18300

tgatagtaaa gaccctaatg aatttgttca atggcaagca aatgcaaaca cagctagcac 18360

tttcgaatta gacaactatg aaaacaatgc agaacctgat acagatgata gtgatgaagt 18420

acctgctatt gaagatgaaa ttgatggcgg ttcagcacct tctcaagatg aagaagatac 18480

cgaggaacac ggtaaagtat ttgcagagga ggaagttaag taatggctaa gactcaagca 18540

gaaataaata aacgtttaga cgcttatgca aaaggtacag tagacagtcc ttatagaatt 18600

aaaaaagcta caagctatga cccatcgttt ggtgtaatgg aagcaggagc aattgacgca 18660

gatggttact atcatgcaca gtgccaagac ttaattactg attatgtatt atggttaaca 18720

gataataaag ttagaacttg gggtaatgct aaagaccaaa tcaaacaaag ttatggtact 18780

ggatttaaaa tacatgaaaa taaaccttct acagtaccta aaaaaggatg gattgctgta 18840

tttacatccg gtagttatca gcaatggggt cacataggta ttgtatatga tggaggtaat 18900

acttctacat ttactatttt agagcaaaac tggaacggtt acgctaataa aaaacctaca 18960

aaacgtgtag ataattatta cggattaact cattttattg agatacctgt aaaagcagga 19020

actactgtta aaaaagaaac agctaagaaa agtgcaagta aaacacctgc acctaaaaag 19080

aaagcaacac taaaagtttc taagaaccat attaactata caatggataa acgtggtaag 19140

aaacctgaag gaatggtaat acacaacgat gcaggtcgtt cttcagggca acaatacgag 19200

aattcattag ctaacgcagg ttatgctaga tatgctaatg gtattgctca ttactatggc 19260

tctgaaggtt atgtatggga agcaatagat gctaagaatc aaattgcttg gcacacagga 19320

gatggaacag gagcaaactc aggtaacttt agatttgcag gtattgaagt ctgtcaatca 19380

atgagtgcta gtgatgctca attccttaaa aacgaacaag cagtattcca atttactgca 19440

gagaaattta aagaatgggg tcttactcct aatcgtaaaa ctgtaagatt gcatatggaa 19500

tttgttccaa cagcttgtcc tcatcgttct atggttcttc atacaggatt taatccagta 19560

acacaaggaa gaccatctca agcaataatg aataaactaa aagattattt cattaaacaa 19620

attaaaaact acatggataa aggaacttca agttctacag tagttaaaga cggtaaaaca 19680

agtagcgcaa gtacaccggc aactagacca gtaacaggct cttggaaaaa gaaccagtac 19740

ggaacttggt acaaaccgga aaatgcaaca tttgttaatg gtaaccaacc tatagtaact 19800

agaataggtt ctccattctt aaatgctcca gtaggaggta acttaccggc aggagctaca 19860

attgtatatg acgaagtttg tatccaagca ggtcacattt ggataggtta caatgcttac 19920

aatggtaaca gagtatattg ccctgttaga acttgtcaag gagttccacc taatcatata 19980

cctggggttg cttggggagt attcaaatag aatagaaatt tagacggatt taaaatccgt 20040

ctattttttt ttgcaaaaaa gtgttgacaa aattaaatac atagtgtata gttatatatg 20100

taatcaaata aaggaggaat tacatggcat tatttttaac atactttgct atttttattg 20160

tttttctagt ccttgtaggt tttggtataa gttatgtatt cgattttctt tcaatgaaag 20220

aaaagaaaag taacataaga aaacaataca gggaattagt taggcaaggt acattagatg 20280

aatacggttt agaacaatat gtaaagtata aaaaacaatt cttaaacgac cgcagacaat 20340

caattgtaac tagagccgat aagcaagaaa tagaccaaga ggaaaaagct ttaaatagct 20400

taataaaaga aatagaaaaa ggagaaatgt aatgagaaag tcagtagtta tttcaggagt 20460

attagggttt ttagcaatta taggatttat tattttatta atgtgtatta caaagattcc 20520

acaaggtcat gttggagttg tatactcagt aaatggtgtt aaggaagata ctaaatcacc 20580

tggttggcac ttaacagcac cttttgataa ggtaaataaa tatccaacta aaacacaaac 20640

acataaatat aaagatttaa atgtagcaac ttcagacggc aaaaatttac aaatggatat 20700

tgatgtatct tataaagtag atgcaactaa agctgtagat ttatttaatc gttttggtag 20760

tgcagatatt gaagaactag aaaaaggata cttgcgttca cgtgtgcaag ataatgttcg 20820

tcaggcagta tctaaatatt ctgtaattga tgcatttggt gttaagacag gagaaattaa 20880

aaaagatact ttagatagct taaatgataa cttagaaaaa caaggatttg taattgagga 20940

tattgcatta tctagcccta aagcagataa gaacactcaa aaagcaattg acagccgtgt 21000

taaagctaac caagagttag aacgtactaa agttgataaa caaattgctg aacaaaatgc 21060

taagaaaaaa gaagtagaag ctaatggtga taaaaaagcc aatgagatta gagaatcttc 21120

tttatctgat aagatattac gtcaacagtt aattgaaaaa tgggatggta aacaacctat 21180

tcaaattggc ggagacggaa ctatcgtaga cgtaacaggg aaataataaa ttagggaggt 21240

taatacctct ctttttttgt ctttaattat ctgataactg taatgtttgt aataaaactt 21300

taataatatt acactaatta gtgctatatt atacttgcgt tatttaaaga tatatgttat 21360

gattacttat gtcgatttac aaataaacaa ttctaaaaaa ttatttaagg agttttaaat 21420

ttatgaagaa aacaattttt gcaacattag cattaggtac ggctattact tttggaggta 21480

ttgctacaaa cgaagctagt gcagacgaaa ttgattataa taagttagca gaacaagcta 21540

aatcaaattc agtagaagta aatacaaaac caattcaaga aggtaactat gatttctcat 21600

tcagtgacgg agaatttact tatcatttct ataattataa tggtaatttt ggatacgaat 21660

atcactcagg ttcaactcaa gtagataata cagtatctag attagcagga gaagaacaaa 21720

cacctgaaca aaaagtagac caacaacaag cgcaatttga tactcaaaat aaacaagata 21780

ctaaaaaaga agtacaacca acatcagcac cagttcaaaa agaaactaaa caacctacac 21840

aatcaactag ttctacagga ggttctgtag cagaacaaat tagacaagca ggtggagacg 21900

aggcaatgat tgaaattgct atgcgtgaat ctacaatgaa tcctaatgcc gttaatgcat 21960

catcaggagc tcaaggacta ttccaaggat tagggaaatc atggagtgga ggttctatag 22020

cagaacaaac taaaggtgca aaacaatata tgattgaccg ttacggttca acatcaggag 22080

ctcttgcata ccacaacgca cataatagtt attaatatat taagactaag attaatttct 22140

tagtcttttt tgtatattat agttgacttc ataagttaac tatgctataa ttagtttaac 22200

aaataaataa aggaggataa tgaatgaata aatttaaaag atggtttcga attaatgttc 22260

taaaaaaaga aacactactt tttaaagttt attggagata tgaatcacca tctttaaaaa 22320

aacctcatgt atttcatata gagttatatg ctaaaagtaa agcagaggca agaaataaat 22380

cacaagagta tatactaaaa aatgcaaaag catctgagga ttttaaattt ttaaaagtag 22440

aggagaaata gttatggatg aaaaaaagga aagtaaacct ctaaatcttc aaaaaattag 22500

agtagaaaaa ggacatacat taagaagcct agcttctgag ataggagtcc attattctct 22560

tatatcttat tgggagtatg gtaaaaagaa accaagaagt gctaatttaa tgaggttaga 22620

gaaggcgtta aatactccag gtaaagagct atttaaggaa ttggaggaag acgatggaga 22680

ataataattt agttaatttt ttaatgacta cagatgatat agatgatact attgaaatgg 22740

tagattcatt tgaattacag gatataaata aagttttagg tgaagatact tttttaacca 22800

ttatggaaat tacagatagt ctaccggaca atcaatataa aatagtatta ttatcttctt 22860

tagacaaatt attgaataca gatagaaaag aattagtaga atatgacgaa gaattcccta 22920

ctatacgaaa acataatgta tctgaattaa aaagggacac agttaattct gtaattgata 22980

gttatatgaa tactaatgtg gaaatacttt atacagagta tcctactatt agtaattaca 23040

gtgtagtagt agattctatt aaagtattaa atactttgta tttaattgaa agtaagaatg 23100

gtaagataga agcaactctg tcagaggatg gagaagactt gcatgagtat atatcagaag 23160

aaggttatag tgttacagat atattaaata aatttgatga tgttgaagat ttatttgatg 23220

aagatgatag tttaattaat ttcttttcag atattgatga aggtaaaaat aaaactatta 23280

aatcgtttat tgagttagtt attaatttaa aataaaatta gtggaggtat ttgttttatg 23340

aaaaaaggag tatttacagt aatagctgat ggttttaaat ttaatgtgat tgctaaagac 23400

aaaaaagaag ttcaagaaca ctgtttcaaa tgttttgatt ttaattatat ttcagtatct 23460

ttctgcagag aagtatattc agattgtgaa ttccctcaat ttatggagga ttataaatat 23520

gcaggatagt gtaaatatat acacagacgg tagctcatct tataataaag gtaaagtagg 23580

ctcaggtgct gtcttagtaa gtaaagaagg aaatataata gcggaaatta gtaaaagtgt 23640

tgacaaacca ggattgataa agtataataa tgttgcaggt gaaatattag cttgttgtta 23700

tggtatcgaa gaggctataa aattaggata caatcaggca atagtttata tagattatat 23760

tggtttaata cattggtatg aaggtacttg gtctgcaaga aatattctaa gtaaaacata 23820

tattaatatg atacgagaat accaaaaagt aatagatata aactttgtaa aagtaaaaag 23880

ccactcaaat gataaatgga atgactacgc tgataatctt gcaaaaaaat caattgatat 23940

ataaaaagga gaaaatatta tgaaaaaaat taattcagta attaaaggtg aaggaaagaa 24000

aattcaaaca acagatgtta gaaaaattag ttattatgtt aaagaccata acccatctat 24060

gactttagaa gatgcagaag attataatgc aaacagtcaa tatttagtca gtgataatgg 24120

taaatttatt gcaaagtata acaaggaaat gaatgcagta ggattttatg aagaatcagg 24180

agaagatgtt aaacatttaa cacatactac tcctgaaaag ctagaaggaa ctgtatttac 24240

aattgaagaa gaaacagaaa ttgatttaat taatgatgta ctccctcaag gagatatttt 24300

aattaagttt acagatggtg atatctactt acctgataat gattcagtat caaatactgt 24360

aaattactta gtaagtaatg attgggatat ggttgatgaa gttatttaca caggcatatc 24420

taaaggtaaa ggggaaaatt gtattgtaga ttttgattct agtatttatc atgaagactt 24480

atttgatgat gaagatgaag aagtttgtga aaattatcct gaatgtaact gcagtaacta 24540

ttgttcttca acaggtgaat atataggtaa ttagtactat attaaaagag agtaaaacta 24600

tttatagata ggggaacttt aaaatatggc aatacaacta aaagagttag actttaagtt 24660

aaaggattat cctaatgtaa gatataacat gggagaacat ctagtattca atgaattcct 24720

tgaaaaagct acaactgagc aattagattt ctgtgaggat ttctttaatg ataatgttga 24780

aatactttgg aatgaaagtc aagccggtac gggtaaaaca atgtgttcag tagcttgtgc 24840

ttatgctgat tatctaaata agaacagaaa attagtattt gtaatttcac ctgtatcaga 24900

agatttaggt agtagacctg gtaaccaaac agaaaaagaa atggcttatt tcatgggatt 24960

acatgatgcc cttattgaac ttaatatgag tcctgaacaa caaataaccg aaatgttaat 25020

gatggaagat aatgtcaaag aagataaatt aggtgaatgt tgggtatccc aaatatcaca 25080

tttattttta agaggcggta atttaaggga tgctactata attataaatg aagcacaaaa 25140

ctttaaacga agtgaactta agaaagtact tacaaggatt catacaaaaa attctacagt 25200

aatagttgaa ggaaatttca agcaaataga tttaaaaaat gaaagtaaat caggttttgg 25260

agattatatg gaatatttta aaaattatga tggtgcggta ttccataatt ttacagttaa 25320

tttccgttct aagttagctc agtacgcaga caattttaaa tggtaaaata atatgttgac 25380

aaacctcttt agttatgata tacttacttt ataataacta aggaggattt tttatgaatt 25440

atttagctaa ggtatttatt aataacagtt ggttggtaaa acttataaca attgtattat 25500

taactttatt tctaagcggt ttagtttata ttataagtgc aatatcatta ttcttatcaa 25560

cagttctaaa tttacctggt ttagtggtat tagcattttt agcaagtgta agtcttattt 25620

tattttcaat agtacataat tcaaaggagg ataactaatt aatgaaatct tatactaaag 25680

taaaaaataa aggattatct ttagacaaat ttaaagatag aggttttgta attcaagaaa 25740

aattagatgg aagcaacgca agttttacta ccgagaatgg tgagttagtc tgtttctctc 25800

gtagaaaaaa attaaatgaa aacgaaacat taaacggttt ttataattgg gtacatgaaa 25860

acatgacaga taaattagat ttatctatat tagaaggtat tattatattt ggagaatggt 25920

tagttaaaca taaggtaaat tataaagaag attgttataa tgatttttat gtattcgatg 25980

tatacgataa ggatagtgaa acctatttac ctcattcaga agtcatttct ttatcagaaa 26040

cattaggttt gaaaactgta aaaactttaa tgattgtaga accatctttt tatcttaatg 26100

aattaaatcc tcaagaaatt caagatttag taggacaatc agatatgact gttaaaccta 26160

atacaggaga aggtattgtt attaaatatt tagatggtaa atcagaacat gatgattact 26220

ttaaattagt atctaaggaa tttaaagagt ttagtcgtaa gaaaatgaaa tcagaagtta 26280

gaagtaatga ttctgtggca gattatgcta tcacaaaatc aagaatggaa aaaatgattt 26340

ttagagctat agaagaaaac agattgtctg aagatgattt agaattagaa aattttggtt 26400

taattatgaa acaagtaggt caaaattttg ttgatgatat tatggaagaa gaaaaagaaa 26460

atatgatgaa aattatagaa aaacaaatta agaaaaagat gccacacatt ttaagaggta 26520

ttctagaaga aaaaggagat actataaatg gttaattata ttaaagacaa agtaatttat 26580

atgggaggtc atctacttaa ccaagctatg gtagattata gagacaaaca gcataagcaa 26640

gtagaaggta ttgtaggagt cactccttac agccctcata aagataaatc aattaatgat 26700

aaggctaatg cagaacaaac tggtttagca gagcgcattt taaacaatga ctttaaagct 26760

atgcaagact cagatatatt tgtgtttgat attcttaatg aggggctagg aacgattgca 26820

gaacttggta ttttactagg tatgaagcat caagcacaag aaactattaa ccatatatat 26880

gataatggag aagaacactt taattatata tcagatagat ttgaaacttg gttaagtgaa 26940

gaagaggaat taatcgtaga aaaacttgaa aatattgtaa ataaacctgt tttaatttat 27000

tgctcggata ttcgtcaagg acatggaaaa ggttatgacg acccggacag agctgaattt 27060

agtacaaatc aatttgttta tggtatggtt ttggaattaa cagacggaga aggatttatc 27120

tcttgggaag aagttttaga aatattagag aaattaggag aacaagatgg ataatttatc 27180

acattacttg agtatattat atgctatatt agttacagta ggttatatac caggtctaat 27240

agctttagtt aaatcggata gtgttaaagg agttagtagt tatttttggt atttaattgt 27300

agctacagta ggtattagtt tttacaattt actattaact gatgctacaa tgtttcaagt 27360

agtatcagtt ggtcttaatt taactctagg tattgtttgc ttattagtag cttcatatag 27420

aaaaaaggat tatttctcta taccttttat tattgtgttc tcattgttac tatttttatt 27480

aagtgacttt acagccttaa cacaaactgt agcaactatt actattatcc ttgcgtatgt 27540

aactcagata acaacatttt ataaaactaa aagttcagaa gggacaaaca gatttctatt 27600

ccttattatt ggattaggat tagcttcgtt gatagtaagt atggttttat cacataccta 27660

tgttcatatt atagctactg aatttgtaaa ctttgttctt atacttatat gttatctaca 27720

ggctaattat tactcaagga ggtaggtaat attggatgac cttattaaag aggggaataa 27780

gtattaccat aaagtaagag caggagagac attatggact ataagtaaaa actatgatgt 27840

agatattaag aaattgcaag aattaaataa tattaaatca gtatcattaa ctagcttaga 27900

gtacgtactt gtttgggtag aataaaccag gaggtttaca aatgagcata gaaaagaaag 27960

aagaagttat agcacataat gaggtagtat ttagaagttt aactcagggt ctatatgtaa 28020

aagaagtaga tatctattca gatgttgtaa gctatactaa agacgttgat gaagctcttg 28080

ctatgcctaa tactattaac ttcaagaatt caagaaaata cgaaaaactt attaggaatt 28140

tagatttaga accactaaat aaaattcaaa aggttatata cgaaactcat ttagaagaac 28200

tttaaaaagt tcttcttttt ttttgttgac aaatatgact tactatgata tgattagtac 28260

ataacaaata aggagtgatt ttaaatggca ataacatata aacaaaaagg attaacagaa 28320

caagaaatta ttaatttacc taaagttaat aaaggatgta tctacatagg agaagaagat 28380

gtattcctta agaagaagaa aaataatata attaacttag gctctaagga attatttaga 28440

gatattcata atatatttag ttttgatact gctacagaaa tacatttatt cttagcacta 28500

tgtggtaata aagaagtaac aaactttaca ggtaaccctt atgaaacagt tgaaaaatta 28560

gttgaaggtg taattggaga aaataaagga agaaattaca aagaatatat tgaatccagt 28620

agagaagaaa gaaaagaatt tcctttatat ggtaataaga gcagaagaca aataaaatct 28680

aaaggttatg tagaagaaaa aattaaagaa ttagaaaatg aaacccgatt atgggggtat 28740

gaagctagac aattagatga gtataaggaa gtagtagaga gtttaaatga tgatattatg 28800

gatattctag accaaggtaa gtacggttta ataaaatcat ctattacttc ttatatgaat 28860

gaagatacag aaaaaggctc aagtaagtat tataaggaaa tatcagattc attgtatagt 28920

agtgcttggt acatgcatcc aggtactgag aataattcat ctttcggtct taaagttaga 28980

catatcagag ataaacataa tatgggtaat aaatgggttt tagaaaataa aagttcattt 29040

gatgttaaaa caggggaggt taaagttttc ttaacagata gccttgttaa taaagaacta 29100

actttaaatt tgtataaaga tgatataagt aaaagtgaat ataaaaatga attgacatta 29160

tctgttttat taaatgttat tttaaaaaat tactcacaac ctaacttaaa tagaggggtt 29220

ataataaaga taatagaaca aacattagaa caccataatt ttgatttttc tagttggtgt 29280

ccggataata tagatgttta tggtcatata aattatagag gagacaaata taggattttt 29340

ataggagaaa attcaacttc taattactta acaactttaa cagatattgt taaaaatatt 29400

gacaaaataa ataacttaga ggaatttgga ttatttgaaa gaaatgcatt actatttcat 29460

atacctaaaa atcctaaatg gaaagttcat gaagctttca atcttacaaa acagacttat 29520

aaaaaattac taactttaaa taaatttgag caaggtaatt acttaagatt tgctaatact 29580

ctatataagt actataatca tttacacaat gaggttaatt tacaccaatt atttgatgat 29640

acttttttaa tggttcgaga ttcaagagat gttactgatg ctttaaaagt taaacctatt 29700

gtaaatcaaa tattatctat atcttttgct aactacaaaa agatgacgca ctatttagat 29760

gtagatgctc aagacagaca acgtataaca ggatatgcac tagatagata ttaccttgac 29820

tatttacaag atttatcaat actaatccga gaagggtata gaacattaga gagtgttaac 29880

ttaacgcctt tttcactgaa gttagagcat gatatagtta cagatgaaaa acaatccatt 29940

caacaacaat tagatgatgc agaacttaaa gctaagtatg ataataagtt agaaaaaata 30000

attgataaaa cttataaatt aaaagatggt agaaaagtaa aattccttcc tgcagatact 30060

gtaagtaaat taaaagatga aggtagaatg ttatctcatt gtgttggagg atatgctaat 30120

agaattctaa agaatagctg tttaatatta ttagcaagat tagaagaaga tttagataat 30180

tcatggttta cagttgaaat acgtattaca gataatggtt atgtgttagg acagcaacaa 30240

tcaattgatg catataaatt acctaatgaa ctaaaagaag cattagaaaa agatattaag 30300

aaaataaata aagaagaatt taaggaggtt gcataaatgg tcaaaccagt tataacttta 30360

gaagtagaag aaactaaatt attattagat tatcttagtt tcttggaaga tgatatgaga 30420

aactacgaag gtatgagaga attatatgaa gaattacaca aaaagtatca acttgccaaa 30480

ggaaactact cagattaatt gacaaaggta taacaatatg gtataataaa ggaagttaga 30540

aatggaaaaa ttccaagaag attatgttaa tatagatgtc agggtgaaag cttatgttcg 30600

tgtaggttat aggtatgaag aagatattac taataaccta catgaattag tcgaagataa 30660

cttaactgta ataagtgatt ctgataatct aattataaaa gatacagaaa ttaaaggaga 30720

tatagaataa tggaaaaagt agaacttatt aagcaatggg caaaagatag aaatttacaa 30780

acaggtaaac ctgaaggtca aatgttaaag ttattagaag aagcaggaga attagcttca 30840

ggtattgcta aaagtaatga ccatgtaaca cgagatagtg taggagatat ttttgtagta 30900

ttaacagtac tatgtttaca attagatata gatattgaag agtgtattga tatggcttat 30960

gatgagataa aagacaggaa aggtaagctt attaacggag tgtttgttaa agaagaagac 31020

cttaaaaaat aagtaataac tagcaaggaa aactgttata ttatgaggtg tacttagata 31080

gtacacttcc ataatatctt tcctttttca ccttgcttgt atccaagcag ggtgtttttt 31140

ttatacaatt taggagggta ttaaatgaat gcaaaagaat ttatgaaaac acaagctcaa 31200

gtagaagatt acttagataa attaaaaata acaattatag aagatgcttt atcagtatct 31260

aaagaatggt ctaatgactc taatgattta ggttatgctt tatctagtct tggtgaaagt 31320

atagggcttt tagaagatta ctataatata caagtagatg cacatttacc tgagcactat 31380

aaaggtagta aggatgttat ttccttctta gaagaacact tttcttatga tggttttgtt 31440

gattctatga tatttaatat tgtaaaatat actacaagat taggaagaaa agatgcagta 31500

gacaaagaag ttcaaaagat taaaacatat tatgtaagat tagaaagaaa tataaaatat 31560

ggggatagta ctcgtgtcta aaagaacaga caattttata tatttctgca aatactattt 31620

ttcagattat ttaccttcat taggtataga agttcttaat cataatgaaa cttcccacgg 31680

aacaatggaa ggtgttaaaa catactatat agcaaacata ctttacgaag gtcaagaact 31740

tacgataact attgatttag aagaattcaa taatgcaact tctatgcaca atatgctaga 31800

aatagtgaat aaacatacat ataattgtat gtttatgtat aatatggata cacatggaac 31860

tgaagacatt gatgactttt ttaaattaat gtatttttag ataaaagtgt tgacaattac 31920

cttaccctat gttaagttat aggtataagg taattttttt attaacaact aaaaaagttt 31980

tagaaagttg ttgacaaatg aaaatacttg tattataata aatgtataac aaatcaaagg 32040

agaatgataa atatggcaaa gcaagatact attgaaagat tagagagatt ggtagaacaa 32100

caaatgcaga caacgaaaga cttagcagat aaactaggag agaaaaactc taacccttat 32160

gaacaagcaa ttgtggatgc aattgtagag aaagcaggaa ctgagagtag agaaataatt 32220

attactgatg ttaaaaaaca aattgaagaa tacgtagagg agcaacttaa taatttacca 32280

gttaagattg aattacaaca agaaggtaaa acaattaaag atatctcagg aatcttccat 32340

tatagatatc aagatatatt aaaactagtt aatcaaaata ttccagtgtt tttaaaaggt 32400

ggagcaggtt caggaaagaa ccatgtatta gagcaagtag cagaagctct agacttagat 32460

ttctatttca gtaatgcaat tactcaagaa tttaaattaa caggatttat tgatgcaaat 32520

ggtaagtttc atgaaactca gttttataaa gcatttacaa atggaggatt attcttccta 32580

gatgagatgg atgcatctat ccctgaagta ttattaattc ttaactcagc tattgcaaat 32640

aaatactttg acttccctat tggtcgtgtg acagcacatg aagactttag agtagtatca 32700

gcaggtaata ctatgggaac tggagcagac catatttatg tgggaagaca gcaactggat 32760

ggagctacat tagaccgttt tgctcaagtt gaatttgatt atgataataa ggtagaacac 32820

caactatcaa gtaatgagga tttagtaaac tttgtacaaa aattaagata tgagaacgat 32880

gaaaaaggat taccttatgt cttttcaatg cgtgcaatta ttaatggaag taaattagat 32940

ggagtaatgg acgatgagtt tgttgtagaa agcatcatct tcaagtctgt acctaaagat 33000

gaaattaatc aatttattag ttctttacct aaagataaca gatatacaga agcaacaaaa 33060

aatcttttag gtatcaaaca agaacctaaa caagaaccaa gaaaaactca aagttcatct 33120

agtgattcaa tggactttga cactatcatg gataaactag gattagaata ggaggaaaaa 33180

acaatgggta gacgattaat agataactca gaattaaatg taattaaata tgatggtcta 33240

cctgatttct tttctgcttt aaaaaagaat agagtttcag gtagagacaa ttcatcagat 33300

acaggtagct acgacttcac aggcactcat agctttaatg aagcctataa cttaatggtt 33360

aaaggagata gagagtctta tgatatggtt gttaagctta aaaagatgac agatgcatta 33420

ttcagaatgg ataaatctgt aaaaagaaaa ccagttgtgg ctcctgaagg ttatcaacct 33480

catgtaccta acgctataaa aggtttacct aattctatga tgtctcaaca gagagttaaa 33540

gcagagaaga aagttattga tgtattctat aattctagta taagttggat ggaagaccct 33600

gagaatcttg cttatagagg agctatactg cttagtgcta tacaaacttt agaaacaaaa 33660

gggtatagta taaatttata tttaggtaag ctatcaaact caagttataa tgataaactt 33720

actgggtttg ttgttaatat aaaacattct tatcaaagat taaatgtttt taaatcatcc 33780

ttttacttag taaacccatc atttttacgt agagtatcgt ttagagtatt agaagttgaa 33840

tctgatatga ctgatttaac acacgatggt tatggtagtg tagtaggtaa aggaagttat 33900

gaaaatgaac ttaaaaaaca cttacttgat aatgctgtta ttttcgattc aagtacaggt 33960

attcatataa acaatgagtc agcagaaaac ttaagagctg taaaaaaact atttggaggt 34020

aggttgtaat ggctaataat atttgggcgg ttgtattaag cattattatt ttactcatca 34080

ttttattaat actttggttt ctttttagaa aaaaggtgag tggcggtagt agtaaaaatg 34140

tagaaattca aaaagcagac gcaggcaaag ataataagga acaagaggta gaagaagctc 34200

aatacagaga acttaatgaa gaagagaaag aaaaaaatga aaactcagaa aaagattata 34260

agtatgataa agaaaaagta aaaaataaac ttaaggagtt agagtaatta tgaagaatct 34320

gattaaactt ttattaatgg ttgtagtaac tatcttgact ttttcactaa cttatgttat 34380

acttaaaaaa gaaacaaaca ataaaagaaa tggtgtagcg ccttttgatt tttcattaga 34440

agaccacatt cacctaaaca aggagattaa ataaatatgg tatatgatag tattatttct 34500

agaacaatgg cggtatcaat tttaaataaa tggattgcag aattaattac agatgttgat 34560

ttagataaat gtaattttac agaagaagaa tatggagcag tagttacaaa ttcaatcaat 34620

aaaatacaag atgtattaat agaaaagaat tatgaagtta cagatggtga gttgtatgat 34680

atcgtttgta cagagttaat taatccaatc aaaaataata cagaagaaga acaaaatgaa 34740

aagaatgatt tattagagca cttagaagat ttagctttta aacatgatat tgatttaggg 34800

tatgttagtg atgggtcata taacttaact gtaactcatt ggttaatgca agatgagttt 34860

acagatgtta atattaaagt taacaaagat gaggatttct atacagttac aattccggaa 34920

agtaaatatt tttggttacc tattacaaaa gagaatctag aaatgttctt aacacaagac 34980

cctattaata aaggagagat tgagtaatgg atagaattat tggtaaacat aacttaactc 35040

aagatttgag attaggtgac aaagtagaag tatatgatgc tcataaattt aaagaaaatg 35100

aagatgggac tattgaatta ggtgacaaag taactgaagg tattgttgta gattataaag 35160

gtgactttac aggtaataca agtgggttag tgacacttga ttcttctgag aaagaattaa 35220

tcataggtga acataacttt aaacttattg aagaaggtaa tttacaagca gtttatgatt 35280

ctgtatctaa aaataaagta gaaagtcttt ctgaagatta tgatatgtat agaaagttac 35340

ttggagttaa atcaggagag ctatcagata tagaagatga actagagtac ttagtaagac 35400

aatataatag taaagtagat aattataatg gactattaac attatctaaa gaaaaagcta 35460

gagaattatc tctattaaca ggagatagaa aaacgattcc tcatatgaaa aatagaagat 35520

tagaattagg tacagaagca gacttttaat aagtctgctt ttctcttata ttagtattag 35580

atataaggag gttggtatat ggcaaatgag aaggaactta taagaatggt taattatctt 35640

attgataata tgtctatgtg gcatataaat tacgctagag ctgttctaat accgagtgaa 35700

gtagagaaga taattaaaga gcatgagaag tttgatgacc tccttaaaaa gagaggagaa 35760

tggttagtaa aaggttcaga tacagataat attgatgatt tagaaactta taatcaaata 35820

atgaataatc aaaaggatga aatgatgata caagaaattg atatctatac ccagggaaaa 35880

acaataaaag ttgataatga acactactct tcagatgaat taaatgaaat tatcaacaaa 35940

atagaacaat cagaagatat taaagtaaaa tccaattata aattactttg catagattat 36000

actaagataa taggatatga ggttacttat gctagctctt acgaggagaa gtttaaaaac 36060

gacctagaaa aagacctgtg atatattagt actgtaagaa taattaggag gaataaaccc 36120

tatggaaaaa gtaaatcatg agtttctagc agaattggca aagagtaata gtcctgtact 36180

aaattcaaaa ccacttcaag atggagacta taatattgaa tttgactatg acggttttca 36240

ctttgagttc tcacagaaaa atggttattg gcaatggaaa tataacgcta aataattaat 36300

tcttaggcta ctttaattag tagccttttt ttgttgacaa aatagaaaaa gtagtgtata 36360

gtaaaagtat aaataaaagg gaggaattat gatgtttgga aaagcaccgg aacatattat 36420

ggaaattata gataaagagg ataatatatt aggagaggac ttaactttaa atatagatta 36480

taagggaata aacctaactg ttaaacgtca ccctcattca ggacatctaa atggttatat 36540

taatgtgcct acgaatataa ccaaagaaca gtttaatagt atagaagatt gttctcatgg 36600

aggtataaca tacgatgaac atgaaggtga ctatagggta ttaggttttg attgtgcaca 36660

ctatagtgat atgacaccat atgctgttat tagtttttct gatagttatt atagagattt 36720

gaaatatgta cttaatactt taaaagatat ggcagactgt ttaaaagaag gtgaatagta 36780

tggacttcta tcaattttta aatcacgaaa atgtacgagt taattctata accctaccgc 36840

aaaaagagtt tataaaagaa aatattgatt atacaaattt agatacagta gatattgatt 36900

ttatgaattc taaacaagca aaagaagaaa tagagaaaat tataagaatt aaaaatgaag 36960

aagaatatga tatggctatg gatgctttag caggatgggt aactaaacac ggttattaag 37020

gaggattgtt atgaaatata ttttagaatt aataacacta ggggctattc tatttaaagt 37080

ttatgaacac tttaaatata aacaagatga agttgacaca acagaagata tttaatgttt 37140

gacattacta tcaacatatg ttattattta aataacaagt aaaaggagga tgttataatg 37200

aacttagaaa aaagtttctt attatcaaca atagaatttg gaagtaccta tcaaggaaca 37260

tcagatgagt attcagataa agattacatg agtttagtag ttcaaccttt atcagatact 37320

atatttagaa atagtgaaaa ggcaagtaaa catacagaag tatctcgata ctatgcagtg 37380

gagagattta tttctttggt attaaagagt ggatttgata atgttcttaa tttatgtgct 37440

caattagagc aagctaaaaa cactagattc aataaaactg ttttagactt gttttatgat 37500

gattttatat ttttaactta tgttagagct aattttaagc ctatagcata ctctgttatt 37560

ggtaatatca ataatatatt aaaaaaagaa gaattaactg gtaaagacct tgttaagttt 37620

tacacattct ataatcattt agaatattat aatgatttat tagatgattt agataactta 37680

aatgttagct ataaagactt tgcaaaagtt aagtatatgc caaaagaagt attagataat 37740

aagagaagta atgtaagtat tgaaaagaaa aaagatttgg ttaataaagt agaaccttta 37800

atccaagaag taaaagataa acttaaatct aatgaatcta gcataaaaca ttataaagat 37860

gctatggaac tagtagaaaa atctttaaaa gataagactg tatcatttct tacggaggtc 37920

tacaatgaga gataaaagaa tacactctga attattatat gatattatag gtaagcatat 37980

acaagaagaa gaaaatatta aaccttatat agaagctata tatgtagata tgatgaatat 38040

tattgtagta gaatatactt tttataatga aaatggaaca agaatgttag gacaatatcc 38100

gataggagag gttatgtaaa tgaaaaaaat tattaatttt ttagtagact ataatataaa 38160

tttcagttat tcagaagata gtttaaatgt tatgggtaac tcgtatctag tagacagaca 38220

cggtacgcag gattatgaaa ttgtaggtaa ctatgaacac attacaggag tattttctta 38280

tcaaacagaa gaagaggtta tagctaaact taaaaatctt attggtgttt gggagtaata 38340

tatgaaaaaa atttatatac tagaggaaga aatagaggaa atggactatg acttgtggga 38400

agaagatact gtgtatacaa caagttacga agttttaggt tatactgatt ccttagaaga 38460

tgctgaatat attaaaaata actatggaac aagtaatcct atatttataa atgaatatcc 38520

ttatctaaca aaagataagt taatagagga acaacgttat tatagatata atagtgctat 38580

agaacttaaa agagttgatg gatatttcga ggtatatgaa ataaatgatt taaatgttac 38640

tgaatgtttt agtattaata aagatgacat aagttttgat tgtccatttt ctatagatat 38700

gttttcatct gacagaaata gtatatttat agaatttatg atgtattcag aatatagtaa 38760

caagaaagat actatagaaa aggaaaagaa ttcttttcta atgaaattaa aatatcttct 38820

taaacacagt aaagaagcgg atataagaag tacatcaaaa attatggatt caattgataa 38880

attatcttga cactaagtac tatttatgat atactattag tatagtagtt aactaaagcc 38940

tcttatataa tgctaaacac agttaacctt tttaacctta taatgagagt aagagcctga 39000

ctatataggt actttgaaat cattatattt tacctatcta ccaagagtag aaaaggtcta 39060

cggtaacgtt atgactctat ttagcgttac tagcaatcct gagtaatggc actggctagt 39120

tgtagctacc tagtcgttga ggttcgagtc ctctctcagg atatttctag gactcttagc 39180

ttaaaggtaa agccaaccgc tcataacggt ttgactgtag gttcgaatcc tacagagtcc 39240

atataccagg ctagcaaaaa acttataggt attagacata tgtgcaatca cttgactatg 39300

atttaatatt atatgtagat ttattgtttc atatctacta agtaaaacct atttctctta 39360

agttactggt tataagagaa ggagtgaaaa tctctaaagg tctagtcaac ctgatgtacg 39420

agagataccg tatgttgccc ggcagaggct ttatatctga ccgtagactc cggatggtag 39480

tatccctcca ttccgtatct aacgcagtga cgagcgtttc aacgatagag ggtctcatat 39540

aattgtcgta aaatattatt aaccgtaatc taagtacggg agctctgtcg gacacctatt 39600

ttcattaata ggtgtttttt tattgacttt tataaataag tatggtaaga tagttatata 39660

caataaggag gagattaaat ggctatatac gtggttcctg atattcacgg tgaatacaat 39720

aaattactga aaattatgga taagataaat gaaaaaagaa gacccgagga tactattgta 39780

tttttaggag attatgtaga tagaggtaaa aggtcaaaag acgttgttaa ctacatgttt 39840

gatttactat caaatgatga taatgtagta gctttattag gaaatcacga tgatgagttt 39900

tataatatta tggaaaacgt agaccggtta agtatctatg atattgaatg gttatcaagg 39960

tattgtatag aaacacttaa ctcttacggt gttagtactg taactttaaa atataaaagt 40020

gtagaagata atttaagaaa tcattatgat tttattaaaa gtgaactaaa gaaacttaaa 40080

gaatctgaag attatagaaa atttaaaata tttatggtta attgtagaag gtattataaa 40140

gaaggtaagt atatattttc ccattcaggg ggagttagtt ggaaacctat agaagagcaa 40200

acagtagacc agttaatatg gtctagagac ttccaaccta gaaaagatgg atttatatat 40260

gtatgtggtc atacacctac tagtagtggt gaagtagaag aacataatga tatactgcta 40320

tgtgacgtag gttctgtatt tagagatata gatttaccac ttattaaatt ggaggattag 40380

atatgaagaa aaaatatttt aaaggtttaa aattaaatga ttttgaaaaa gaagtttttg 40440

ggttaaagag aaatagaaag tataaaaaaa tgaataaaaa attaggaaga aatgaaccta 40500

agtattggaa ctatgatatg tctttttttg ttcaattata tgccgattta aatgccttta 40560

tagaaagtag tgaacgttct tgtatagata tggaatggaa tacttttgta gatgtagatg 40620

gtgaagaaag aacacaaata gatatgataa aacatatttt aagcttaata caatattatc 40680

atgaagaaat ggatgatttt gatatggata aatatgatga gcttgaacaa gtacaaaata 40740

aaatattaga taatttcaaa attgtgttac catcattatg gaattaaagg ggagatatta 40800

tgaaagaaac aagcagatac attatgtttt ggggtaagga agatatttat tctaactttt 40860

acccaataac atttaaacat aaagggagaa catttaataa ctcagaacaa gcttttatgt 40920

ggcacaaagc gcaatatttt aaagactggc aaatagcagg agaaatatta aatgctaaga 40980

atccgaacca tgctaaaagt ctaggtcgta aagttcgtaa ttttaatgaa gagcaatgga 41040

ataaggtaag atatgacatt atggtagaag tggttaaaga taaatttatg actacacact 41100

taaaacggga aatactcgat acagatttac gtaaagattt cgtagaggca tcaccttatg 41160

ataaaatatg gggagtaggt cttaaagaaa gtgaccctaa aatattagat gagagtaact 41220

ggaaaggtca gaatctatta ggtaaagtaa tggaagacgt tcgagtacat tgtgtataca 41280

acaaatacaa ataaaaaagg agaattaaaa tgaatataaa atatatagac ttagtattag 41340

aaaattgtga tgtagtaagg ttagaaccta aagatgtaaa caggttccat atagaaggtg 41400

ttacagaagg aataaattac tatggtacgt ataaaggaac ttcacacata aatagaacac 41460

gtcattgtac ttactttggt attctcatta ataagcctaa agaaatacct caagttggtt 41520

ttgcttaccc tgataataca aatgcttatg aaatgattac agcgtactca gatattacag 41580

ctatagatat tatttatgaa aatggaacaa atgaatacat ttatgtagac ttcaatgaat 41640

ataatgataa ctataatatt aatcaaaaga atgagtatta caatagtatg ctagaagtaa 41700

ctattacgga aagtaattct atagaagaag aggaggaata ataacgatgt ttaaaaaaag 41760

caaggaaaat ataactataa cagatgaaga aaagaaaaaa tgggatattt ttgaaaatac 41820

tttaagagta tttttaagag aaactcaaaa tacaattatc acctttaaaa atggttatag 41880

cttctcctct agaagaatac ctcgtgaaga gttatttaaa gatgaagagg tttacgaagt 41940

tatatattat aaacctaatg gagataaatt aactagaatt acagatggtc ttattctagc 42000

taatgatgga atggcaagtt ttacaaactc agttacagaa gaagaattac aaaaccctgc 42060

ctgtgttcct gaaattctgt atcttagaat acaggaaaaa atagatgaaa tagagcaaga 42120

aaaatttatt agtgaaaatg tgaaaaaggt actaaaaaat agtgagtata ttaaaacagt 42180

tgattatata gaggaggaat tataatgata tttaaaaata ataaagtaga aaaagaaata 42240

aaaaaagata ttaattttat aagaatttgt gatgtatcag ggtcttctat tatcgtacaa 42300

caaaaagata ctaaaactaa tttaaattct tttattgagg gtcttgtttt taatggtgct 42360

aaaactttag aatctaacaa aggggataag tctctatggt ttaaagataa tattactcat 42420

atagatactg tatattacaa ggaggtagaa gatgaataaa acattcttta aagccttagg 42480

aaagaataca ctagagtatt caatacaagg attaggatta cttgtagctc ttcctttaat 42540

gctaattata ttctctgtat tcttagcatt cattattggt attcctgccg gtatcattta 42600

tggtttatat gtattaggca taaacaatga ctttataact cagctcatac ctgttgcatg 42660

gtttataatt ctatatggta taacaagaac tcaagataat aaaaaacctt tcgttaaact 42720

aaaattaaag gattatttac tatctatctt atatcttaca attattacag ctacaagtgt 42780

tttagaaagt gttttactat ttaaattatt accctttact ggagacatta gagcagttat 42840

aactctatta tcatttatgt tattcttagc tgttaataga ggtatctgta agattgttat 42900

taaaagttat aaagaatata aggaggactc acaatgatag aaataagtgt ctcatggact 42960

tacctaatat cattcctact actatggtca gccggaacat tatatattaa ctaccttatt 43020

tatagaataa gattaacaaa taaggaacgt aaagaaatga gtaaagaaca ccaccgtaat 43080

cgagaagaga taaaacaaag gatagaagat aggagggata aataatggct tatgaatata 43140

aaaataaaat tcaagatatt ataactgaca acgagaacta ctggtgtatt gataatgaaa 43200

aagaattaga agagttacaa gaagtctatc aaaaagcaga agccttcgat gaaattgtaa 43260

atgaattata ctatcaatta cagaatctag agagttggga caccctagac caaaaagatt 43320

gtcaaacatt aaaacaaata ttagaagaga atataaagga gagtaaagta gatgattaaa 43380

tttagagcat gggatgaaga atcacaaaga atgtttgata ttgctaggtt tgactttgca 43440

gattacacag tatattcaca tctatttgct tgtgatggtt atttaggaga aaaccttgaa 43500

attatgcaat tcacaggtct tagtgataga aatagtgtag aaatatttga gggagatatt 43560

gtagaaatac aagatattaa tggtaactat ataaactcag gtatcgttaa aaaaggtaaa 43620

gggtttttct ttatagaaaa ttatgaaggt aataagatta ctttgcttag tgatttctat 43680

ttaaaatcat atacaaatac cttagaatta tgtgttatag gtaatatcta tgaaggtaga 43740

ggagaggaaa aacaaatgga gagatttaag gtaaaacgta ctattactac agaagaaata 43800

agatatatag atgcagaaac agaagaagat gcatggtata gtgtagaata tgaagatgag 43860

ggaacagaca cagctcattt taacgcagaa tatggtgaat ggacttacga aaaggaggaa 43920

aagtaatggg attagatttt gaagtaattg gtgtaacttt aagtaaaaga aaagtagaac 43980

aaaaaggatt acaacatttt attaacaatt cgaaatatag aaatcagtta gaaaaaaact 44040

attatcaaag atttaatttt gaagatgatt ttagaaaacc aggatatttt atggaactct 44100

tactaagaga tgcagaaata tattatgata aatttgatga atttggtgac gagggtgcat 44160

atgttttaac tcatgaaaaa cttattaacc tactggaatc tgagtttaat gaaaaaactt 44220

ttaaacatac tcatgattta gagtattatt atagactaat gttatacctt tgtaatatag 44280

aacatcataa cagtaagttt tataattttt atttaatcat gcgtataagt gtataaggag 44340

gaaaagtaat ggaatttttt atagatagaa caagcacaat taataaaaag cctatagaag 44400

gtgcctacat aaaggaatta gaacgtgtag accaaaaagg taatacattt actatagaac 44460

gttggtgtgt agaaattaat agcttagaag agcttataga gttaagtaat caagagggag 44520

aagttattat taatactaga ggtgactcac cattttcacc ttatttagaa atatatgatt 44580

actatagaga ataggaggat aactaatatg aaactatatc aagtagaaca tgataattgt 44640

gaaccctatg aagataattt ccatttcagg gaagatgaga tatacacaaa taaggagaac 44700

ttaattaaac gtattaaaga agaaggatat aaagaaagta taactcatag aggagaacaa 44760

gagttcatta aaggagaccc aagaggattc gatagaatgg atatgattac tattcatgaa 44820

ttagaagtta ttagtaatac cttagaggaa gaataatatt ctttctcttt ttttatatat 44880

aaaggttgac attctaacac ctatccttta tactaagtat ataatcaatt aggaggtcat 44940

acattatgaa tgaactacaa ggtatacaaa cactatttaa taaactagag aagcggttag 45000

aagatacagc ttattgcggt aataaacaat taggatataa agatatacta aagattcaaa 45060

taggtcttga tgacacagct tttgatgttc tatactatga tgaactagaa gcgtatttag 45120

aaaaccctca agatttccac ctttattatg atgattcaag agtaaattgg attgaggttt 45180

gggagcattt aaatagaata gaacaggaac taaataatag aagcgtactt atatagttaa 45240

gagaagagag gatatacctc tcttcttatt ttagatatgt tgactttata tttagaggct 45300

ttatatttgt actataaatt gttgacttat tataagggat gttgacttac tttctatatt 45360

ttatgtgttg acttttaaat atttattttc ataattttcc ccaaaaaata agtacctggg 45420

tagaccatag gggtatatag acccaccccc attgagtggc attttacccc tgcccccgta 45480

taatataaaa aaattatata ataagataaa aataaaagag ggattttatt ccctctattt 45540

gttatttaaa atattccgtt ttgttttttt atttctacga ttcttttttc ttgtctttct 45600

aatttgttaa ctaaaatatc gtaagcttca gtgtattgtt ctcctctagc aatcattctg 45660

tctaataccc aaataatatc ttttacgtct ttttctaatt gttcctgttc taataagttt 45720

gtcatgttca tcatttcctt ttcttttaat ttgttaagtt cattatatca tacaaattaa 45780

aagattgtaa agcgttttga taaaataatt taaacaaatg tttgtattag ttctttaatc 45840

tcctcagtag ataatgaaga taggtcataa gcttctctaa gctcttctaa gctaccatat 45900

gagccattac tatatgaagc ggtgttattg ttcatctgta ttgttccgct tccctgtgtg 45960

cgtctgtgta gctcttctgt gatgtagtca tctatgatta cattattaat attattatgt 46020

tcctgcagga gtttgctctt tgcactctta acgacattat gaacctctct aatgctcatt 46080

actcatcatc accagtcata acatcaacta atatcattag ctcttgctta gcctttattt 46140

gcttctcgag ttgtttattc aactcctgca tagtcttttg agtgtctgct atttgctcat 46200

ctaataatcg aaccgcttcg tttaacgctt tgtttaccct tcttgtattc atgttttaca 46260

tctcctctaa aatataatct aaatcatttc cgtaatagct tctatattcc tcttcttcca 46320

tatccataac aatatttgcc cattgcttag cgtgactcat tgcataaacc tttttgccct 46380

catctgataa ctccaagaat agtttagctt ctagagagtc attatctatt gtccaatctt 46440

cctcgatagt actatcaaac tcgatagcaa ataatgtttc gtatagtttt ttagcattgt 46500

ttaattggtt aattttcatc ttcaacatct cctgattaat tgtattagag aagcggttaa 46560

gcttctctgt gtgtgtgtct gtgtgcttcc tatagtgcca ttctttgcgc tacaatctca 46620

accgctagat taacatattg aaccttgtcc gtttcttgta gttctaattc aacatcatcc 46680

atagcctcat agattgcttc cgcctggtct gcttcgtcca tgtcttcaaa tccttcaacc 46740

tcatcagaag caatgttttc tgctaggtca aattgaatgt tattatattc gtcttcgtct 46800

tcaaattcta catttgcata attctcgagg tctctcataa gctcatagtt caataagtca 46860

taatattgtt gtcccgtaac ctcttcaatg tattcagtga tgacggcttc aatgtcctgc 46920

tcgtatctat caaagaagtt tactatgtct gatgtgtaga ttaatttgcc aattgctccg 46980

ctttgtactc catgagttgc tacgtcctta actgtgcttg cttcttgtcc ttgtaaaaat 47040

gcttgtattg tatttgtcat aataatcaaa tccttttctt tttttatttt ttctatactc 47100

ttatattacc atctatatgc tagggtgtca ataccttttt taaattaatt tccaaccgat 47160

gtcgtttaca tattcttcta gttcttccat agtgtccgca aatttgattg actgtagagt 47220

gtcagaagtc accacaaccg cttcttggtc gtaaatttta ctgtctagga tgttattatc 47280

taagtggtcg atgtactccc aaattagggc ttgttttacg ctctcaagtg ttgctatgct 47340

aattacgatt tcattatctc tattgattaa tttcatttgt atcaattcct tttcttattt 47400

gtatttttta actatcttaa tattaacatg taaggggtta agtgtcaacc ccttatttag 47460

atttttttaa acagtttctg agttcttttt taattcgatt tgtaggtcta attctctaat 47520

ctgtgcacaa gttctttcaa atctgtcata gtatccgttt gctaattcct catctgattt 47580

tctttctaga ctctctaatt ggctttctaa ttgtttgatt gtttttgtca ttgtaatcat 47640

ttccttttct ttagtatagt ttaatattat catgaagcgg ttagtttgtc aacagcttat 47700

tttaattttt ttccatgctt acattttctt gaccatataa gtcacaccat tcttgatatt 47760

tggcttctgc ttcctgcttg ttgaatgttg catacataaa caattcttga ttaactaacg 47820

ttacaatttc gtatctcata attatcaatt cctctcattt ggtatatatt aatattaaca 47880

tggtaggggt tgaatgtcaa ccccttttat taagtttttt tatgcgtatt cttcgattgc 47940

ttcatgtact aggaaagtta aaaccttacc acctaagaat ttacattctt gatttgcttc 48000

tgatagagtg tagtcataca ccttttcaaa gctaaccgct tcgttgtatt cctcttgtgt 48060

cataacgccg tccataacgc tattatccat cataaggata tattccattg ccgtatcttt 48120

tactatttct aatctgttca taattatcaa ttccttttcg tttttttgtt tttctaacta 48180

tcttaatatt atcatgtatt tggaagtttg tcaacacctt tttaaaagtt ttttaaattc 48240

tttttttagg ttatttcctc cgcttcctta ctacacttta tatattacac catagtacaa 48300

aataagtcaa ccctttttgt aaactttttt aaataaatta tttgaaagaa agttcttgac 48360

tttaggggca aaatttgaga taatggaggt ggacggtatc tttaagataa ttaaattatt 48420

atataaaaaa agaaagtatt gacaactagt agcaaaattg atataatgga cttggagccc 48480

taaaaactct ataacttttt tatataatat tatttttata ctaaaaaaag tcgtcactct 48540

attatctcat tttttaatac atttgtcaag cataaatttt acacaaaaat accgcttcaa 48600

gaatttaatc cgaagcggtt tatataaatt ttttatttca tgatatttaa taatgttgtg 48660

tgtaattctt caatgtctga ttggtctaca atgtacaatg tgccattaac tttgatactg 48720

atagcctctt gtccgtaaac ttcaattaaa cgtcttacaa taccttttaa ttcaactgtc 48780

atgaattttg cactgtcttg cagataatac cactcaagat ttaagttgtt atcttccatt 48840

atacgttttt catcttctga gtaccattgc cctttaattt ctgtaatagt acaaccgccc 48900

gcttccattg tgatgctatt tacatattct ttaatacgtt tgtctgcaat ctcaacgccg 48960

ttgttatctt ccttaggtac gataatttcc actttatttt gtaatttgaa cattttcatc 49020

aattcctttt cagtttattt tgtcgttgtg cttgattacc ttacaattta ataataacat 49080

ggtagggctt ggaagtcaag ccctttattt aattaatttt cgtaaatgaa taagtttttg 49140

cttggtaagt atctaaagtt tggcgcaatt cctaagcaat atacaagtgt gtttaaactg 49200

tcacggtctg aattgctttc accgtaatat gaaacaatct catttatgct taagtcaatc 49260

tcaattgttt gtgtacccca gtgacgcata atcaatttgt tgccatcaat ttcaattgca 49320

tatttttcct caagctcacc tttgctatac ccgtcaaagt aacgaccgtt taaaattgct 49380

tgccctttgt tttctgcttt ttctaataag ttttcgatag ttttgataaa tttcattata 49440

atcaattcct tttctgtttg ttttttgtta tcttaataat atcatggttt caagtgactg 49500

tcaatagttt ttttaaactt tttttattta ggtcggaagc ggtcaaccgc ttcccttacc 49560

ttgtatctta atattaacac gttgtttcta agttgtcaat agttatctta aaaaaatatt 49620

tatattatct tgtagtagta aaaaccagtt tacaagttta ggcgtgactg ttgcaactaa 49680

accacctata agaagtaaaa taatgttatc cgttttttga gttgatttga ttgttttgtt 49740

tttccattta aattttagca tttgtattgc ctcccttgtt ggtataccta tatattacta 49800

tagaaaaaaa cataagtcaa tagatttgtt gaaattaatt taaaaaactt ttttgaataa 49860

aagtattgac attaagaccg aattattata taatggaagt ggagccctaa aatatgtata 49920

atttttttat ataataataa aagaaagtaa aaaaaagttt ctgaaaggtg ttgacaatta 49980

ccattattca tagtatacta tatatagaaa gtaaaacagt ggtaacccgc ctaacccttc 50040

cactataaat atgacaaggt ggggagaaag agcggtttta aaaccattta atataaattt 50100

tttatataat aattttttta tactaaaaaa ggtcttcact tcattatacc ataaaaaata 50160

caaccttgtc aacagtcaat tttacactaa tttatttatt aaataatttt tttatataaa 50220

ttaaaacaaa taaaaatccc taggaattta atcctaggga attatataac ttttttatac 50280

tattgaattg cacttctatt atatcttaat tccataaatt tattataagt ttgtttactg 50340

ttaaatataa taaattcatc tgattcattt ttaaatatat tgtgtacagt ttgataactc 50400

atatatccat ttttagctaa attccataaa gtttttatat cactgttatt tttttctatt 50460

ttaaatttca taatatcata tcctttttat ataatttttt tatttaattc agaagcggtt 50520

ttaattctta accgcttcta tttctttaac attagatagt gcgtaattta atgtatcttc 50580

aatattatag ctttggaata ctacaccacc gccaaaccat ttagcagtat attttttaaa 50640

tccgtatagc ttattgatgt tgtcatagtc tgctagttta atgtctaaat ccatgaaatg 50700

cactacataa cgtgggttgc cgtttgtgtc atttggaatt ctgaaagccg taatgtcttg 50760

cccttgtact gtgtgagttt cttcgtataa gtaatttttg tttgtcataa taatcaaatc 50820

ctattctata atttttttat ttaatttcgt tatttgctat gtggcttgtc ttaacttcta 50880

tactaatatt aacatgtatc ttgtaaggtg tcaacacttt tctttaactt ttttaaaaag 50940

ttttttaagg tgtgttgcta tcccttacat ctattatatt actacatctt aaggggtatt 51000

gcaaccccta tttttaaatt tcttcaataa attttatttc tttattctgt accccgtaaa 51060

ccgtattttc atatacgatt ataatcacgt catcaattac atttactaac atgctagccg 51120

gttgcgcttg ccctctcatg ctcattatag gcgttttacg gaacatttga acagtgtcat 51180

agtttccctt aatgcttacg attaaagccg tatatgtgcg tgtgtggttt aatgtgcttt 51240

catataatgg cggttctgta catctgtatc cgtccatttg ccacaactcc attatagtgt 51300

ctgcaatttc ttttacatcc atttaaatca tctccaatta aataattttt ttatataatt 51360

ctaatacctt gttgctgttc tcttgctgta cctttatatt actatcatat gattaataag 51420

tcaataagaa aatgtaaatt cttttaaaaa acttttttta gggctaaaaa ctactattat 51480

atagaagaaa aaagaattta aaaaaaagtt actcaaaagt attgacttct gaataactat 51540

actgtaatat attaagtgta gtaaggcaag ggacaaaccc caaccgcttc tacaaatatt 51600

atataaaaaa attatagaaa aggatttgat ttaaatgaaa ataaactata ttccaatgtg 51660

ggacaatgaa gacgtattac aatatgcaaa atcacaatta ttagtaaatg aattagaaac 51720

aaaagaaatt gtattcaaaa attatcaaat atcagatgat attgataacg gaacagataa 51780

aaaatattat gaaatatatg aaagtaaatt ttatgtagat gaggatacaa caaaagaaga 51840

atttaataat ttaattatag aaaatgaaaa actagtaaaa gactataaaa cacaaaacgg 51900

attaattaaa aatttaatta aatcacaaca tgaagtaaat gaatttgaat acaatgttat 51960

aaatattcta taaaaaaatt atacaataca ctgggaataa tatcctagtg tatttctttt 52020

tgcggtctgc tattattccg attgtctata tatagacacg tgaagcggtt aaaataaact 52080

aatagaaaac agagggagct acttagggta aaatcacacc gagctactta ggcaattcct 52140

agggagctac ttagtgaaat tagagagagc tacttagtca aaattacgtt gccaagcctt 52200

taacctaatt atctctccta ggtcatgctt acttatatgc tctcctttat aatagtaata 52260

attagaatag ttaccaaaaa attcaggaac acctaataca gctacaggct ctctataact 52320

attaccaata agaatatctt tcctattcat aagaccttcc atatccctac agtatacaac 52380

tgatacaaca taagaagagt ctatatcttc aggtaactca ttcttataat attttaaata 52440

tctatctcta tcattaagcc aaggtaagaa ctcatctctt aagtaatccc tagactcatc 52500

tgaatatata actatcatcc tatccctcct tatcttgtgt gccaagtatg ttcaaaatca 52560

aatatacctt gtttacgtat gtctactaat aagttaagta tatctatatt cccactaaaa 52620

agacggtagt tagataattc tatatctttt actaatccct cttctatcat tttgtatata 52680

agttcaatag tttctaattc acttaaatcc tgtgctacta ctttacctga ttctttatgg 52740

taaattatat aagttattaa ctcttgcatc ttaacttaac ctccctatcg tatcgtattt 52800

catcctaagc atcctaagtt atttttatcc tattatccta agcatcctat tgtatctcaa 52860

actttttagg gtctaaccta ttatccatgt agcttacgtt tgaatagaaa tacaccctgt 52920

gattctatat catccagtag tctactcata tctataacta caccacgtgt attcatgtaa 52980

tattctaaat taatatcctt aactatatct ttctctatca tcttgttaat aaggtgcacc 53040

aatgatgcat caaacaaatt cttagctact actttagctg tttctgtatg atacactgta 53100

tacgtgaaaa actgactcat atttatctcc tcctaccact ctacaatatt accttcatta 53160

ataagtaata caccttcttc attaaccgct tcaattaatc taactacatc tatgtctata 53220

cagaattttg tttcatacgt agtataatct gttgggtata tattacttat atccttatgc 53280

caagtcttat ataagaagtc aattaaatca cttctactta agtaatcctt aatagttttc 53340

atactattct tatgatatac tgtataataa gtttctacca ttaattacat ctcctttaca 53400

tattcttcca tactatctac acctattgat ttaccattac gatactcatg tatccatgtt 53460

tcagctataa atacacatac atcatcttca aactgaggat gtaagctcat ttcttcaacc 53520

tcatccttgg tatatctaaa aggactaata tgtacatcta atcctgggct tctattaaca 53580

tcataagtta gttctcttga agcggtctct ttaatccatt ttaattctgc tttgagtaaa 53640

tccctatcat agttttcact gagatatatt tctatcatta taatctctcc tttacaaagg 53700

caccaatatt acatgataca cctctatagt aactacattc taaaccattt ttatttatga 53760

gtatacaaag tttaccttca tcaataatgt ttgagttaat ccttacatca tctctttctc 53820

tgaatacatt cttctctata catacatccg cttctccttt tcccattaaa tagctaagac 53880

ctctgctact acaattatca atatgttcta atactttctt taaagccttg gcatcatatt 53940

tttcagttaa aaatattata atcattataa catcccctct acatacttac gtataataat 54000

aggttcccca ttaacaaatg ccttacagta taattcttct ataagaatac ataagtcatc 54060

tttaaagtga ggaagatttg ataaatcaat cgtattctcc tcatctatag ggtcatcggt 54120

ttcaatcact acatccttat catagtctgt attaatacca taagttaatt ccctacctac 54180

agtatcatta atatattcaa gtaaactcct taatgtttct ttattataat cctcttttac 54240

aaatatatga atcattatat ttcctcctta catcatcata ttcgttttcc taggtacaat 54300

aaacttacct acttcgttaa tatataaatc accgtttata taataccctg cgtgattacc 54360

tacaaatact tggaagcggt cagacccttt acttactcta tcacaagaat ccattaactc 54420

tgcaaaagat aagtcagtgt gttgtacaat aacatccact tcctccatat ctgatatgtc 54480

agtactatat gttaagtttc tattctccgc ttctaatttt tctaaaagac tctttaatgt 54540

atttttacta tacctattag cacagtatat agttgcttgc attatatctc ctccttttat 54600

acaaaatata ctattaaata aatacaagag cataatgagc atacgtaaat aagcagtgca 54660

caccaatcaa acgtaactga agtacattca tagtatatta agtatatgtt aatcaataat 54720

aaaatactaa aaaatatagc cattatatct ctcctttcaa ttctaaaccc tctcagcctt 54780

ccatagagaa gcggtcatat atttctagac caatttatca tgaactactt aaccgcttct 54840

taaatgagct ctaaatgcca agtatagcta atgcaccact aacgataata actaacatta 54900

atatactccc agtaatcata aagattttat cattagcatc atacttaata ctcttgaacg 54960

tctcatagaa aaataatcct acacaaccta ttgtaactat actaacaata gctaatagta 55020

taatttgacc tatcatttta ctacctcctt tataaacatt ctacaattat catttctaaa 55080

ccaatcccat ttataactac aataccaact accatagcat acatgatacc tttttcatct 55140

actttgtatg catcatgtgc aacataactg aatgcaaatg taccgaaggt catcatgaat 55200

ataattaatg gtataatcat aataactccc cctagaattt ttctctaact tcttgactaa 55260

gacctgtacc tgttagaaag ttaccaccat gtataacctt agtctcatct ctagtaatac 55320

atataataat acagcctgga taatccatag acatcgtacc atatacatgc agaacatcac 55380

cttcatactt catatcctct atatttacat ccgcttcttt attattaaac ccataactta 55440

actctcttga agcggaagtc tttataatat tcataatagt agataagtaa tcagtattat 55500

agttttcccc taagtatata tcaatcatat tattcatctc cttatatact aatcatacca 55560

tactacttag agtatgtcaa cacttaaata aaaataaccc ctactggaat agaggttatg 55620

aatgacacta atatgcaaac caaaatcacg accatagtct aggtctgtag ggaaccaccc 55680

ctacaatact aagtataaca taccgcttca tagttgtcaa cctttaattg taattgaagc 55740

ggatatcttc taatattctc aattaaacag acctaaatga taaggagcta cttacgattt 55800

taggtacatt aaaactttac cctagggaaa acctaatact ttttagtaat tttaaaaata 55860

agaatcccat acctataggg ttctatattt tagtaccact tttcctaggg tctaaagatt 55920

aaaattctag attagaatga ttctaatgta ataacctaga ttataccacc attatataag 55980

attattatat aataacaata gattaatacc gcttcagtat agattaaggt tacaatacta 56040

tagtttaagt attgtataat aaatgaatat tataactata gaatacatag ttagggaagt 56100

cggtagtaag taataggctg agtgggagag gtaaaagttt tcccaggctc taggtattgt 56160

ttagttactt tacttggttt taattgattt tattggtttt acttgattaa ctcctctcct 56220

atatatcttc cttattagtc aacatactcc tattagtcct aatgtctatt ataataatct 56280

gttatagcta aacataccat aaatgtaatt aaagggacaa ctcctataga cataataatt 56340

atttttgcaa acaagtccat atgtttcctc cttataaatt atatacttta aacatcttat 56400

ccatagtaat ctataaactc tacttcttca aaacctctta tctctgataa tcctgaatca 56460

tctgtagatt ctattgttac tgtttcattt ttaaagttaa taatttctac attaacaatt 56520

tgttgggtta tttcatccca taccttttta tatctattta tcatattaat tcctcccatt 56580

taaaattaaa tactagttgc ccttctctta atagagttaa agtttttata aaataatatt 56640

gatttcttca atttcaatat taataacctt atcattaaat tcttcgttac ttaaaatcag 56700

gataagcatt gaaacatcct tgtcagtacc tttaattacc tgatttgtca cactattact 56760

atactttgta gtaagtgtta cttctaagtc catttcttca atttggtcat ctgttaagtt 56820

aaaaataatt ttctttgctc ttaatgctgt tctgtatctt tgtaataatg tatttactgt 56880

ttgtttagct tctcttgcca ttttacattt ctcctttatt ttttaattat ttttaccatt 56940

atcctaaata gtctctaaat tcacttaata tcgtttcttt gttactaata tatagtatat 57000

tgtcatactt agtatcgtat atttcaatat tttcacttaa atacaaatca tctttgttac 57060

ttattaactc agaaaaacca ttaaacttaa gctctacatc atccatccca ccgcaagggt 57120

aataatcaga atagctaaac aataaaaatc tatacattaa aattcctccc atacgaatcc 57180

gaatatacca tcattttgta cttgtcttac ccattcctct aataccttat gaagaactga 57240

actaacactc tgtgtattag gaattttaac aacagttgtc atataaagac cttgtggtat 57300

atgcatttct atagaatctg agtataactt atcaatatct atagacacaa cttctgtgtt 57360

ataagtaaaa gtgagtctca taatgtaaga cattaaatct acattagata acctaacttt 57420

agtttcatca ggtaatatta gatatttacc ttgtttactt gtaaaataat ctactaattt 57480

tgtactattc ataattgttc ctcctggtca tattctatag gtaatagctc atacccttta 57540

ttactatatc catgtaatct ttcccaataa tagacatcta aacaatgttc catactacct 57600

tgtgcaatag gttctcttct tttgtataat acaaacacat cataagtcat tttcccactc 57660

ctttagtttt tgtatatatt cttcaatata taataatttt ttagctctta ttataatagt 57720

aaaatcacca ttctttttaa cccaataatt tgtttctttc ttacggaatt ccattaattt 57780

taagaaatga acaatttccc ttttacttct gaagtaataa ggtaagatat catctttttg 57840

acctacaaaa cctttaattg ttctctctag tatatacatt ataatccacc ttctgtaatt 57900

tctattttta tttctaatcc tgctttcttt aaactatttt tttctttaac aaatatccaa 57960

tctatatcaa tattattttt aagagtaccg ttaggtctta aatatgtcat aggtatccat 58020

atattttggt tcgtattatt tattgtaaac ctcctagctt ttgcttgacc atagccccta 58080

tcaatttgtt gtaaaggtat atttttataa tattgagtat caaaattacg atttgtacta 58140

ttataactat ctttcttatc atgtataaat tgtgtcatac tacttagtct cctttatata 58200

ttaattcatc attcttgtac actttcttac ctacattaga gaaaccatca cctttatcaa 58260

acttatgata gacctcttta ttaagatata cacggtcttc tcctttatct gtctctaaat 58320

ctacataata tttcatttca ccaatgactg ggacagtaac gctacctgat tttatataag 58380

tagttggttc tctgtattca cctaccccta ctactttacc ctcatggtta tcaggagagc 58440

aacctcctag taatagagaa atagtaattg taagtaatac tattaacttt ttcatatttt 58500

catctccttg tatactatta tataatagat atctacctaa gtcaataggt agatactaat 58560

ttatttatca gctacgtact cataaaaccc aatatcttct ctaagtttat cttctcttaa 58620

ctgcttaaca tctttagatg atactgtaac tccttcgaca tattctacta ggtcaatgaa 58680

tgctttttct aagtattccc tcggacagtc ttcgttttcc caatagttaa ccctttcttt 58740

ttcccagtta ttattcatgc tatttctctc ctttcaactc tttaatttca agtgtaacta 58800

ctttatctac atactcttct atagctttat aagataactg atttaaatat tgttcactaa 58860

ctcctaatat ttcagtaacc ttttccaggt gtcttactac atcatctaac tcagtatcta 58920

cgtaacctag agattcaaac atttcatcta tttcattttt gtcaggctta atttctttat 58980

attcattata aaattcttca gtagttctaa agtacttttg tctatttctt ttaatatgat 59040

ggtagtctat taattgtctc tcaccattaa ttgtatcaaa ctttcttaca ctaaatgata 59100

cgacatgacc gcttatttct agtttaactc catttaattt atctataatc attttatttc 59160

tctcctttta cttatggctt taaccattcc catttttcaa accatagtat ttttataata 59220

ttgtacttat gacctacttt atagtcatcc gaattgaact catttacttt taaggtaata 59280

accttttttt tcttaggatt atctacaccg ataagaatca catctacata atctacatac 59340

gcaaaccctt catcaggagg gtaagtagaa tactcaaacc tactataata attatgtttt 59400

gatattttat aaatcatctc tttcactcct ttatttacta tagttatact atataataaa 59460

aaactccctt tgtcaatagg gagtttaaat atttattaat tatttttaat tctttctcta 59520

atttcactta gtgactggtc tctgtatagt ttaccgtctt caaatactcg ttctaattga 59580

tttaaattct gtttatttaa ccactggtga agatttaggt tatcttccca tgaaccatct 59640

ttgtaaacag ctactctacc tttatgtgaa cgtttaccac tgtctgtttt aggattctta 59700

tagataagtt tctcttcatt tttaatctta gcatatgttg ctttgattgc aaacccatga 59760

gtatcacgag tattaaattg ataagagaaa ctacctacac ctaatacaac gttaatactt 59820

gcaaaacctt ttgcttttaa tccctcacaa atttgctgag cacgctcata gttaatagaa 59880

tcaccataga ttaatcctac atgttcatct agtactttat atcctttttc agtttcagta 59940

cctccaaaag tatcccaaag tacttctaca gcacctttac gttcatactc agtatctgca 60000

tcagggtcac cacaaataat cttaacaggg tctccactat caggtctgat tactacttta 60060

ccgtcacgtt ccataataat atcttttaac ctaggtaaag ttttagtcat attaccccaa 60120

aagtcccaag tatcagatac aatagagaat actcctgttg gataaatttc tgttaacata 60180

cgtttgtatg tttcatattc atccattgtc tcgtaatcac catttgcaca cataaccgaa 60240

tgttcagtag cagggatgga agcaacaacc atttcttttt ctacatctgc attgtagtaa 60300

gattctaaaa agtctactac aggtacagtg tcactacctt taaaactaat tgcatgtcct 60360

gctgagctta attgagctgt ttctaatgaa ctcattccac gcatactaaa gtcatgtcct 60420

tggaaatcta ctgcttcaat attacctact gtttctaatg catacttatc taacatttta 60480

cgcatatcat atgctagtgt agcactagtc attgcttgcc atgtttgagt actaataata 60540

gtttctaaat agttagttag ccaaaagaaa tctgagtgag tattttcaat tgtcataaca 60600

ggtgtattag ggtgaactac tgtaccttcc tttaaagctc ttacatcaat aggtaagtaa 60660

cctaggtcat gtaactgctc taaatgttta gcgtcagggt tttcttgtcc taatgtgaat 60720

ttaactgtac gtttatattc attaataact tcctctttag gtctattaaa gaattcttta 60780

ttaaacatat caataaaata ttttttaaca agtgattgaa taccaaatac tacaatatta 60840

tcactatgtt catagtactt attacttcta ggtacaagtg tactgtatac aatttctgta 60900

ccttcagggt attgttctct gtgacttaat ttataaaagt cacacattaa tgttggattt 60960

agcatagttt attcttctcc ttttatagat atctttcgat actctttatt tgtaattctg 61020

ttccgttttc ttgtgctcta tgtttagcaa tagctttttc ccactggcta tcaggtaaca 61080

ttgagtctgt tacaataata tctttaaatc ctgaatcgag taatttaccg tttttaaata 61140

ctttctctgc atgggtcaat attaatgata cactatttac cttaagattg tcaagggctt 61200

ttttacaacc tacaaatgta ccaccataac ttgttaaatc atctagtata atacagttat 61260

catataaagt atttttatcc ttaacaattt taataccttt aattttacct gtttcaaaat 61320

cacgtttctt ttcaccatat acaattgaat aattttcaat attggaatcc cttaagatgt 61380

cttccacatc agataggtac ctgtcataag ctcctttatc cggtagtaca attgttacag 61440

gttcttcaga catttcaata tattggtcta ataatttaag tgtgacacct atagaaacag 61500

aatttttaat tagattttct gtgacagggc tatgagcttc tgctatcaac acagatttat 61560

aattaagatt gttaataaat tctgcaatat acttaagact aaacatattg ctatgacctt 61620

cttctactct atccattcta ctataaggta aatacgcaat ttccaggaag gaaacattat 61680

ttttaatacc taattgttct agatacttag taaacatata caaatgcatt aagtctgcat 61740

cagtttccca tttaaagaaa acatcaaacc atagttctcc ttctactaaa tattttaggt 61800

catcatattt aaaattaatt tctccattag ggaatttctc cggtacaatc gttttatatt 61860

ttgtatccat ttctgaaaat actttaatca tcgtggtaac tcctttttat agtttatttg 61920

aaatacattt tctgctgata tacttttagc aacatcaaaa aacccttgac cgatatttaa 61980

cattgtgtct acatctacta atgttaaatt ctccttatac atttttctta atatttcttt 62040

tacttctaag tatccacctt taaaaactaa ttttcctgtc tctctatgat gtatttccca 62100

aacttcctcc atcataatac cttctagtct ttctctagta ctttttaatt tataagctaa 62160

ctcacctata tcaggataca taacttttca tctccttaat ttcaattcta agccatttta 62220

aggtaccata tagagcactt aacctatttt aagtactctt tatagtaaaa ttcttattgg 62280

tctcctctgt acgttctaac agtcttatca tacttagatt ttttaatttc gaacataatc 62340

ttattataaa tcatctcgat actaaataag aaagcaccaa acattacaga tactaatcga 62400

ataacattct ctctaacatt taagtaatct aagtaaaagt aaagacctac agccatacct 62460

agtacaatta aaatacctga tagaccagta ctaaattttg taacataaaa gtaaataccg 62520

tacataccaa ttaatactgc tataatatta ataactaatt ccatttataa tccctccgtt 62580

gtaatctgac ttccaaaatt attcatataa taaacataag cttgcagttc atccaaatta 62640

atttctttct tcggataaga tgctgatgtc atttctgaaa taatatgaac tcttttctca 62700

gtaacactaa tgtggacgtt ataacccccg ctaatacaat ccgtaataaa atctctataa 62760

ttcataatta ctccccctta aaataaaata gctcctgcta cagtataatc atctctttct 62820

aaacaatact gttgtaactt aatgtagtca tttttattaa tatttgatac atcaatttgt 62880

aacacacctt tatcagtatc tgcgttaaat tccttattaa caattgctaa aatacttcca 62940

gtaaatctaa ccttacatgg cttattcttt ttaataactt ctagtacttc tttatagttc 63000

atttataatt tcctccttta taatataaat cctacaagta atagtatagt accgatacca 63060

aagattgtaa ataacatttt taaatctgtg ttatcataat atttacctaa aactaattta 63120

tatccgctag ctaaaaataa tattaaaccg acaattaaaa ttataatatc taccatatta 63180

ctaccccctt attaatctat agatttatta gctttatcta acgctaatag taattcagtc 63240

attacatctc tcttttcaaa atcagacact ggtaaatcaa gatatccttt aataatttta 63300

tgtgcctgca cataacctaa atcttcaata tcttctgatt tacttactac ctcgtttgct 63360

ttcttgtgta atctttcaat ttgtttgtta ttcatgttaa cctctccttt ttctttatat 63420

ctaaagtata ctataaaaaa tacctgttgt caacaggtat ttataaattt atttattttt 63480

tcgtaatacc aataatatca cactcaggca tacctaagta aagatttagt atttcagagt 63540

caggctcgtc aattaacact attgagtctt tgtaaccttc tctttttttc gatgtaactt 63600

taatagtcgg ataatcattt aatagcactc taaccatgac tgtgtttgat actataagag 63660

gtaaatcata ttctatagct aaataagcca ttgttgatgt ttttcctgtg catctatcta 63720

aaagttcaga aggtaaaact acaggtactc ccttatcaat agatgattta aacactgagt 63780

agtattcttc attaatataa ggctttagtt ttctaagagc tgtctcctta ttaatatcat 63840

caattgcttc aataaataaa tcatctgtct ttatctgctc ttctacaatt ttcaatactt 63900

tatctttaat ttgagtaatg atttctttag gctcttcatt ttcgtaagat ataattattg 63960

gagattgtaa tttataatta tttctgtatt gtattgaggt ttcaaactct attagatacc 64020

ctacaccctt atatctacct ttctcaagtg ttacttttat attgtgtttt tgtatattta 64080

tatgtttcat atttaataat gaatttataa tagcatctga tttcatttat tttacctcct 64140

taaagtatcg agttaattcg atatctgttt tcgtttggtc tcctacaatc tcttgtagtt 64200

tattaccaat ctctgtgtat aattccttag agcttagtgt ttcaggttta ttaatcgtaa 64260

tatgtgtatc taataagtac ttgcctgata gagtgaaaac tacagtatct aaaaacaata 64320

ctgattcatc catagtatct atatctaaaa tatatgatga actagcttga gtgaatctta 64380

ccttataccc ttctatttgt aataccttat cctgaaaaaa tcgtgctatt gactctgctt 64440

tcataattac ttttcctcct tattatcttt tggaataaaa atctgagata aatatctatc 64500

ctctaatact cgaatagctt cttcatactg agaaatcatt gcatcgaagt gtcttagttg 64560

taactgtgat tcatactgtt cctcaccctt agtttggtaa tctgcaaagt tcttttcttc 64620

tttaattaat tctgctagtg cctctcttaa agcttgtgat ggtgttgcaa tataattttc 64680

tccgttgata tttgattcat attttgccat ttatagttcc tcctttaatt tctactaaac 64740

atacttccta ctgcactgat taagataaat atcccgatac atacccaaat acttacttta 64800

acaccaaatg cagataacac taattgtaaa atccatgcag gtaaaacaat acctaatgca 64860

ataactagaa ttacaattcc taaaatcaaa ataaaatgct tcatcagtta acctcctttt 64920

ctatactata acatagtata ccttaattgt caacacgtac tttcaattgc ttcctgatac 64980

atactaagat attcatctaa cacattcttg tatactataa ttattttctt acgtctcttg 65040

tgtttaacac taaaacctaa tgccttcatg tactctataa atattttctg ctcaggtttg 65100

gataaagtaa actcttttgt tttcgggtaa taatagacat gaccgatagg tctatacttt 65160

ccatgtttat tatgtagctt ctctgttttt ataactaaat ttttaattgt ttgctttcta 65220

attctagttt tatataaatc tttacctttg aatttctcca ttatattcac ctgctctact 65280

tataatatca tcaaaactat aggcaatctt accctctaca tcataaccta cattaatgtg 65340

atactctgta tccattaact gtgaatgtat atgaccatga atattaaaca tgttttttct 65400

acctattagc gtagggtaat ggcttagata aaatctttta ccatcctttt taatcatatc 65460

tgcgtacttt actgtaatac cattacgttc atatagtttt ataagtttct tatggtcatg 65520

gttacctgca atcagtatca tctctttgta atttagtcta ttaagtatat cttctacttt 65580

agacggtttc atattgaaaa agaagtctcc aatattataa acaatatcgt tatttgtaat 65640

tgtattattc cacaactcaa ttaaaccttc atccatctct tctactgagt tccagtgact 65700

tcttgtaggt gcttcaaatt ctctaatatt atcatgacca aaatgtaaat cactaacaaa 65760

ataaatcata ttatgtcctc ctttttaaat agtttgtaat atcctgatag tacatttatc 65820

tttcttaatg gtttatcaaa agggaatgtt aatactctac cttttttata gtgtatttgg 65880

tcataaccat cataagctgt tgtatctcca tttttatcac atgcctgtaa tacataacct 65940

tttaaataaa catttggtat atcgtaagct ttaatcacat tatttttatc tataaattcc 66000

attgtatcac tcctaaataa atttactcaa actatataat ccttctctat ctagcttata 66060

tgaaagtaat ttcatatttg tcataggttc tgtatcgagt ggagataatc cttctacctt 66120

agaaacatct acagatgtta attctaattc atacatcttt ttaattgtat ctacaacttt 66180

actcttttta tcctctatga ttgtcttatt agattggtta tggattaact tatatacatc 66240

ttcctcatac cctaaacgtg ttacatcacc cagtttttct ttcgttctat tataaatacc 66300

aaatgattct ctttcttcta ttaattgttt tttctttcgt aaaaatatag aataaataaa 66360

aatacctaat aaagataaaa taaatagtaa accaaaaatt aatgcaaata ttgctaacat 66420

tattgtctca actcctctaa atattcttct actctttcaa tttcttgtct aatacctttt 66480

tcatagatac gtactgaaag agatgaatga cctagtattc caataactaa attattgttt 66540

gacatactaa actgttctaa attatcctca ttaatttggt tatcctttaa aactactaca 66600

tcattatcta ctaaagtacc ttcagttgtg taagataatc cttctagctt aaagtttata 66660

aacccaagaa aatatcctaa atcctctttg gtctcttctt tgtaaaatat aataatacct 66720

ttcataagtt aacctcctta tttcttataa tattagtata taataaaaga aacctaatgt 66780

caataggttt cttaaacttt ttatttattt gtattcataa atcttttaag gtctccagtt 66840

tcaaaagtac ctattttaat atctttaact tctttacgct cttgttcttc taagtaaaga 66900

atacctaatg agttagctgt tgtaactatt gttcttgtaa tctgtcctgt attatagttc 66960

tcttgtttaa ttttaccttt tttatatcta attagtgcta cagtatctaa ggctaacatt 67020

aaaaaattat ttattgttaa tgttagaatt ttcttttgtc tactattcat cttttgtttt 67080

tcctccctcg tcttcaaaac gttctatttg ttttttaagt tctttattct ctgcttcaag 67140

ttcttttact ctttgctcta actcttcttt agtcatagaa ttcctcctac tttataaacc 67200

ctttaagttc taaaatagca taatactcag ggttaattac gtgtaaacca ccccaaataa 67260

aactagcaac aatactaact aacataattg agtatactac aaaagctgtt ggatggttaa 67320

atggtacctg taattttttt actagtaccc aaactaataa agataataac acccctagta 67380

aaataaatcc taaacctact aacacctggt gccataccat agctgaaaaa ccttgcccta 67440

atgcactact tgcttgctct actgctttgt tcattttaat ttcctcctta ttaaatatct 67500

aatttctcat aacaatcttc acaaattaaa cctgaatctt ctttttcaag gtatgcttta 67560

ttatctccca tgtatctata tccatcttgc cacatatcaa tgtacccttt acacatgtca 67620

cactcagtgt cttcatgact tgtattgtta attaagtcac tactaccaca ttcactacat 67680

tcttcaggct cttcatcatg accccaatct tcaaaatgtt tgcaatcctc acactcgtaa 67740

gttaatacaa aatcttcctc aaattcaaaa tctctttgtt tcattttagt ttcctccttt 67800

agcttgttcg attaaatctt ttactgcaaa tacctcaggg ttcagtattt ttataatatc 67860

cataggtatt ccatatacta agtatataaa tgcaaatagt gatatgattg atgtagctat 67920

tacataaaac aaacctatac ctgataaatc ttcacaatct tcaaatagta aactataatc 67980

atcatctcta tctatatcat ttaccttttt atgttggtta ataaataact tccataaaaa 68040

tacagttaat gctataacta taatacctac tataaggtct ataataccat atataatttg 68100

atactttact aaagcttccc aagcatgttg gctaatatca cctaacttac ctccaagaga 68160

atcaattctt tctaatactt tatcttctaa cttcactata accactcctt ttttagtctt 68220

ctgagttcca tccgttaata aatcctaata caaagaatac tgctaagaaa aaaattatga 68280

ataacatagg tcatacctcc tctttatttg ttaacttaag tataacctat gtacactcat 68340

ttgtcaacag ttatttcttc tttttactta atttcttttg ttgctttttt aatgaatctt 68400

tttgctgtgg tgttaacatc cctatctctc ctcatcaaat tcttttgttg tttttataga 68460

gtattctgta ctaggatttc tttcttctaa tctttctttt agctcttcta atactgaaat 68520

atctctgtgt gctctaactt gcctaggtat tttcttttta cctactttat catagtaaca 68580

taaagcatac cactcattca tttatatagc caccttattt aatatttttg atttctaatt 68640

caaattctac taaagagttt ttagataaat ccgtgatact gaatacatta ttatctcttt 68700

taataaagtg attgctaggt gtaattcttt caggttcatt tttcttaata agtaactcta 68760

actgagagaa tagtctctca ttaactaagt tgttgatatc ctcttcaata tcttttctag 68820

tcaaatagga atcatttaat ttgtagatat tcttattaac attaccttta ccaataaaca 68880

tattatttac atgacaatta cttctgtcac catctttaaa acatacgtga ccgttcttaa 68940

ggtctcctcg tgattggcta atcatttcag gaaaggtatg tgcaataaca acacctcgtg 69000

aataacttgt tttcttaggg ttaattaaat atacaatctc atgagcagtc cacttaggat 69060

gatttttcct tgtctgtgtt cctttaatta gttttcttgt tactttattt ctaattaaac 69120

ctgaagggtg ggcttcatag taaccattaa aaggtactgt tctccatcct ttctcaggtt 69180

gtactgtatg gtctaaatgt tttcttcttt taatcattat ttttcctcct tttgtaactt 69240

cttatccaaa gttacactat aatcctcttt ataaaatcct ttaattctac ctttatgacc 69300

tctagcatgt ttaacgtatt tgtagacagt acctacaaat tgaattcttt ttccttgtaa 69360

ttcttttagt ttttcataaa aagaaattcc aacttcaata ttaacatggt caatagatat 69420

accattaatc tttacgtcag taactgttat ttttacacat ccatgttgat tacctttatg 69480

tcttctacgc tttgagtact taacatcatc taagtaccct ttaacattaa cttgttttct 69540

attaaaaggt tttaattcct ctctcatctt tccacctcct actatagttg taacttatga 69600

tagttaaaca tatctttagt taatttctta gcaaagtaat taatcatata ctgtgttgaa 69660

gttccttcaa gtttatcata atcaaagtca atacatgtga aaactacaaa gcttcctaag 69720

tttctattac cttctcgaac ttcaaataca agtgtgtcat ttttaaaagt tttaatttta 69780

aactctttat ttgtattaaa gcttttaggg aaattccacc attccttagt tgttgccata 69840

atctcaattg cgtttgaaaa ttgtcttcta attgtttctt tactcatatt aatcatctcc 69900

tgtacttgtt atacttatag tataatacaa aagtttagaa gtgtcaacac ttatttttaa 69960

gaaaatataa gaagatttat tatcatccct aatgcacata ttaacgaagc acctgagtat 70020

agtattgatt ccttatcatc tttaactttt ctagctaaga gtaagaaaag tagcatacct 70080

gcttgtagta aaaagaacca tccataaaaa cctgcccaaa tgtttattaa tattgcagta 70140

ccagttaaca tatagctcat atcatcatta attaaatcaa taataacgac agctataaag 70200

gcaacagaag ctattgtcat taagtccata ggcattaatc ccaattccag tcttccatta 70260

caaaatcaat gtaagcttca ttttcttcat ctataatttg gtctttaacc gactctattt 70320

ctgaaataac aaactgtaaa gaatctgtta gtctaccaag agcaatacgt tctgttgttt 70380

taataggtaa acgtcctgaa tgagggtgaa ctaaattatc aataacttct accgcttttg 70440

tatcaccact tacaatttta atagttcctt catcaaaaaa tactttactt gatttacctg 70500

tacttgcact tagtaaactt gcaatactat taatcatata cactttcgta tcttctaaat 70560

tttttagttt ttcatttaac attttatttc ctccttataa cattaagtcc tcgtttaatt 70620

gacccatagc ttctataaaa tcagggttcc tatctagcat atctacagga ttatcaacat 70680

cccactctac acctgcatgt atatctaaat atgaaattgg tatatctata acaaaatcta 70740

catcgtctac tgcatctgag tagctgtaaa aactatcagg gtcattttct gcaataacta 70800

tattgtaatc attaaactct tcttctatgg cttttctact aagtcttgtt gctgtcattt 70860

taaccaaacc atgttcatcg tttaaatgat tttgtattgt ttttattgtt tcttttagaa 70920

ctcgtttatt tgtttcttca taatggataa ctaatatatt catctatatt acctcctaat 70980

acaagtaaac tactgtcata gcattatgaa tagacttcat aatccaaaat aagaatatta 71040

ataaaactcc tgcaccaata actcctggta accatctgtc tttccaaaat tcaattccaa 71100

acatcactac aaaataagat acaaatcccc atataaacca taaaattcct aataacataa 71160

taatcattcc tcttttcttt gttttagtta taatcttagt atatattaaa aggcttcatc 71220

tgtcaagcct ttaatgtaaa gttttttatt aaaatattct aaagctttct gagctgttga 71280

tacctcttta gtaaattctc taagttcttt catatcaagt tcttctgagc cgatacgcat 71340

tacaagctct acaccttctc gaccattatc aaatataata ggtgaaaatt ttgttttatg 71400

cttcttatct acttcagtaa caataatatt acctactagt cttacaacta tagtataatc 71460

tctatactta tctttgtaaa gtacatttgt ttctttagtc atttcttttt ctccttaaat 71520

caatgtataa tgatttttct cttattcttt tcaacatttt ttccttctct tcaataggag 71580

tagaagaagg tagtttattt tctaccttct tcttttgttt aggtttaccc actctatctg 71640

attcactgtt tataaagtca ttaaaatcca tagtaaaact ccttacaata aaatattatt 71700

tactgctaac catattaaaa gtacaataat tccaaacaca attgtactga ctactgccat 71760

tatcatggag caagcttcta catctaaggt agttttactt agtaataaca tcattagttt 71820

atctaataaa agggtcatac ctttaaatac taaataaagt attgccatag ctactgcaaa 71880

agctataata atgtatatta caagtaatac actatcaaat gaacttatca ttggtttttc 71940

acctctttaa ttactattgt attgcttgta ttcttctaaa tcttcttgtt ttctaatttc 72000

actttcttta tctagcttct ttagtttttt gtactcagaa attgttaaag gtctattagt 72060

tattaattga cttttccaat ggtcaatatc ctcaggtcta tcaaccttag tataaaaaac 72120

ccaacttaaa ctagtttctt taatttcttt accctcatca tttgtccgat aaaactcagt 72180

aacacctaat aaatttaaat accagtagct atagtattta taatcttttt ctccaggtaa 72240

tctcaaatag taagaattat tccattcagt atattcccat ccaggaaata cagatttaaa 72300

tattagtata ggtattaaag ccgatagtat accgtataca acgatgataa taagtaatat 72360

acttattatt tttactaggg tactatccgt taaatctttg ataatatctc caagcattaa 72420

tgttaatgct atagctatga tattatttat tataactaca ctaaagtata ttaagtctcc 72480

taatgttcta cctgcaggac tagaccttaa ctcagaccaa cttatcttat ccattttagt 72540

attagcttta ttctctaagt agctctgttt tacttcttct gaaactttca taataattcc 72600

tccttttcag tataatatta ctatacacta tataaaatga tttgtcaata aaaaaagcta 72660

acaaattaat gttagcctgg ttgatttact ttacgctctt taatagaaaa agatacacgc 72720

tttccatctt ttgtttctac ttggttttct ttaacaaatc tttctgctaa ctcttgagtt 72780

gaaaagtaaa atgattctcg gtcagaactt ccttctgttg gtgtcataat tacagtcttt 72840

acaataactt catatacact aaactttggt aatgttttat ccataataaa ttccccttta 72900

ttcattttct ttatccttaa tggcttgggc tactgcatca ttgattactc gtgccatgat 72960

tctgtgtgca gttctaataa ccaagaatcc tattcctaag aaagtagcta atacaaatgg 73020

gtcaatagtg gcatgagtta taataataac taaactagct acaccataaa agaataaaaa 73080

tcctactgta taaattagcg ctactactaa taaattaaat aagtacactc cgataaattt 73140

actcaccata tctctcctct tctagcaaat tagtaacata ttcattattt tgttctaatt 73200

cttgaatgta atcaaaagct aattctaaca ttttactttc actaatgtga ccttgcttat 73260

aatactctaa ttcattcttg aatttatttt gtgctttaat actaggtata accataatat 73320

ctctcctcct ttagtaatac tatactacac ttatttgttc ttgtcaatag tttctttatc 73380

ttttttaaaa agttctttag ataacttagt atgctgttct actgccttat tatatctcat 73440

atatttaaga tgaaggatat taatctcact aaaagttatt tgtcttaagt tatgttttga 73500

tacgatatct acttcatcat taggatactt aaatatctta gcttggttct gctcagcatt 73560

acttaattcc caatacattt taatagttcc cctgtaggat actattttat tcattttact 73620

tactttccat gtacctaagg ctaaaacaaa cataataata actgcaggcg ctaaaggtga 73680

ataaacagca ttacctgtta caaaatattc aagtacacgt aacattacaa gaataaagaa 73740

acttataatt agccctacca taactgaatc taatacctta gtagatacat agtttcttgt 73800

tcctttagaa cgtatactat ccttataatc atctacttct tttaacctta attgtatgtc 73860

cctattagca tcttcaatct ctttaactaa cttttccctt tctgtcattg tcctatctcc 73920

cttggtctat ttgagctatc gacttgagta agaatttata atttctttta aagttttttg 73980

cttctatttt accgaactta cttctaatgc gttttttagc ttggtaagct gtacctcctt 74040

ctagtatcgg atttaataag tctacactca attctaatat tctttcctcc gaaagctcat 74100

cgtctgaaac taatcttaca aacgtatcaa atctttctaa atctgccatt tccataatta 74160

atctctcctt tctatatatt aatataccat aaaacaaaaa gagagtcaag aattaacttg 74220

actcaatcac tgttccataa gtaataaatg atagcaataa agatgacgac aattaatgtt 74280

gtcatttaag tcacctcctt attacttttt ggttggtact gcttcaaacc tatacttatg 74340

aattggatta tcttccccat cttcagtata taccctaata agcttgaaat ttaccccttc 74400

tttaaatcta cttgttttgt agtacctcat atctatgctg ttatctcctg ttctaccggg 74460

taggtaagta ggtaatgttt ccataaagta agctttatta ttttccactt ttttaagtgt 74520

atagtcaccg acaaattcta cgttcttttc ttctttaatc tttcccgtgt ttgataactc 74580

agtagagact tcattctttg ctttgtcgtg atacatgaaa gaagatatat aataagatga 74640

aatagaaaaa gccaaaacaa taactactag tatatagaat atacttgctt ttcttgacat 74700

aattaacacc tctataaaaa atgtctcctc ttacagttat aatataagag gagactaact 74760

attaatctat aattttaaca catttatcta gaagtgagca ataaatcatt ttgtcactgc 74820

ctactaattc tcgtaattta ctatagtata taagcaaatc atcggaatcc gttaattcag 74880

tatagcggtc gagaatttca ttaatacgac tttcaaaaaa cttactatcg acaatttcta 74940

aatgggatat ttcttctcct gttactaacc tcaggtcttc taattcaaca tcatatatct 75000

tcttgtcatc tttaatatct tcaagtaact catgctgttg atattcgaga tttgtattat 75060

taatacgtac taaaggtttt aaaacatgag aagtttcata aagatttcta ggcatttgac 75120

ctcttaaatg actaaaatct tctgattctg ataataaccc tgtactgata ccaatatatt 75180

gacctggttg tacttctgta gaacgatatc cttttggtat tgtacaaata taaagcttat 75240

tagattcata tactggctta gatgcaggaa aattatcgac aacatctttt aatccctgag 75300

catccaataa attttctttt aactcattta aagtatataa cttttcttct tctacttcag 75360

gttcttcaga aggagtcact tcttcagtga actcctcttc agatacccat ttctctttat 75420

tacgcatgtc atttaattgc tcttgaaatg attttttatt tctcatatta taacctcttt 75480

acttactcaa cgacaatatt atcctcttcc aatgctttgt ccttaagttc agattttctt 75540

tccacatggt ataaatccaa gatgtaatat aaaccattct tatgaagatt atatttacta 75600

taaatgtcct gtaaattcat atactggtaa tccttaataa tctctttaac taagtttttg 75660

tctttcaaga tatgtgcaat tctttcttct acaggtgatt tcttgttacg cttacctact 75720

ttatagtagt ttaagactgt gtaaattaat cccatactta ctttagcttc ttttgcaatg 75780

taatctaaag gtttaccttc attataactt gttacaatga attcttcttt aggtgtgtta 75840

aactctcttg atgtttctaa tggtgtatac ttgttttcct ctttagttaa taattctttc 75900

tcttcttgat ttaatgaaat aaacataatt gttagctcct ttatttttag tatacatata 75960

ctatatcata tactttcaga actgtcaacc ctaatcttta actttattta atttattttc 76020

atcaaagagc attgtttctc ctgtaagtgc atagaaataa ttaccattat tatctgtaag 76080

tatttcttta atcatagcaa catctttttc ttggtcttct cttgtatatt ctacaatagt 76140

atctttagga tacttaacct tagttgtttc tgagtattct tcatctaggt aaacatcaaa 76200

tgttataaga gagttaacag atacattata gagaacccaa tgtcttttat agtagaatga 76260

ttctttatcc aagtctctag atacttcagt aaatttatgt ttccctggac tcattctaac 76320

aaggatatcc ccttggttgt cttgtttaac tctcagtact tttccgtttt ttaagtatgt 76380

catgatatct actatatcat caattggctt agcatcttgt attggattta aactaatctc 76440

atctgccatt actctaacct cactatctaa attgtatagc ctactctatc aagagcttct 76500

tttaattttc tactttcttc atctaaatct agatttttgt agaattcatt ttgtagttct 76560

tctaatcgtt gtaatacctc ttcatgtttt tcttcaggta taaactctaa taatacttca 76620

ctcatagccg tatcttgagc tgtttgtttt aattgtaatt ctctaagtcc aataaatgac 76680

atacctttta attggtttcc ggtaatctta tctttagttt ccattgcacg tatcattaaa 76740

gcagggtcta aagtttccac aaattctaat ccttttgcac ctttagaaat aatcatatct 76800

agtacttgaa tatcatcata gattgtttgt gtgtgatgtt ttgattgttc aaatggagaa 76860

agttgctcct cttctaatat aggagtttct ttttccttaa tatcttttac gcttgttttt 76920

ttacgtttat caattaattc tcctaaatcc caaccatttt caatagcttc ttttctttta 76980

cttttgtatc ttgtaatagc tgatttagac aattctaaat catattcttt acatagttct 77040

ataatatcat cataactcat atcttcatct aaagcattat ctacctttga gcgtagtaat 77100

ttattatggt atagcttaga taatatactt tctttttctt tatcaggaac aacttttata 77160

tcactgtttt tatttcgtaa attctttttt ctagccatta tatagctctc cttaactgtc 77220

atttatttat ggtaactgat gttctgaaac cagtttaacg aacataaaca aaccctacaa 77280

catcaactgt ttcaaggtta cactatataa tatatcacaa tttctgttat tatttcataa 77340

tatataaatg taatataatt ttattaacta ctttgtataa aattttaata tattttttaa 77400

ctatttacaa agcctttacc taaaagatat tttaactttt cataaatata atttcacaga 77460

aactaggaca agtgtaaaaa acttaactag tttctgtatt aaaaacatta tgaaaacttt 77520

ctatataacg tatatttata cttaattatc acatatttat aacattctat tagaataaaa 77580

tagttaatgt gtcgtcacct tttaagttaa ttactagctc agttaattta tgagtctcat 77640

cttcaatttt aacatgacta gatttaatat ctttagtatt aactttaacg tatgactgac 77700

cacaagcaat agtaataata tccgtatctt ctaaaaagaa tatacctgtt tcatgtgtat 77760

tatataaatt gaagtagtag tctttactta ataacttaac acaaaataca cttttactta 77820

ttaaactatt atttctaaag aatgctaata atttatcagt atctccttta gataacttat 77880

ctttcttaga tagcatacca aatagtgttt gaccatcata taatttctct gtagtaagaa 77940

gatatacaat atgtgtataa ccgtttaatg gtgactcttc ttctagtttg tatagacgta 78000

acttaatatc tttagctata gtaatgtctt gatatgttac ttcaggctca ataaaagtac 78060

ttacaatatc cactttctta gtagaagtta agttagtacc tctagcaagt aaatctgtca 78120

ttaatccagt ttcttctagc tcttttttaa gttgttcagt tctaacatat ccaagacctg 78180

aggaacctaa ttgattgttc atacctacta cagcctgttt cattgattcc actacgttta 78240

ctttattaac aaactgcaga ccttcatctt cattacctat atcttcaata taagcttgta 78300

ctgtaacacc tgaagggaag taatcactac cataagatac acctagtaat tctactttac 78360

cacttgttaa ggtcttatat aaggttttaa tagttgtagg tttatctaca tgttctgaaa 78420

atccttttaa cattatttat ctttcctttc tgctttaact ctatgtcttg tcttaaatat 78480

aaatatcaaa tgtttacctt taaatgttct ttgctcccta atactacatt ttccaataag 78540

ttttttacct ctatgcacat aataaactct atctgtgtgt aaaatagatt ctgttttctt 78600

tcctttttgt ttaagagata gataagtttc tttaacatca agtgcctcac taataagatt 78660

taatgcatat tttttttgta acttaacatc tgtacttgtt acattatcaa atcttttaat 78720

aaaaagtatt ctatattcaa taacagatgt tgtatcctta ggttctgagg gctttttagc 78780

aaagaaacta ttattagctc ctgttaaagt atttgttggg gcaaagttaa caggttgatg 78840

ctgtatctct ttacgtctat ttggtttgtc tttgctcatt gattactctt accccatttt 78900

tcatagtatt aaagaaatct tcttctataa cttcccatct attattataa ccataaataa 78960

cacctcttgt aaagagttta taagtattat ctacccctac ataatgaatt atttggttaa 79020

taatagcagg ttttttcatt cttttacttt gagttgtttg agagaagttg ccttccccac 79080

cactatacct accgctagca ttataactat accctgttct ataagtatga gggtctctaa 79140

tcaacctaag tattgtcatt aaatttgtct tccatagtat attcattgta tgataagggc 79200

ttctatgtgg tgatggtgta gcatctttat atttacctac aacaggttta cctttaaaat 79260

taatgtaact cattatacct acatgattat gtttatatct tttaagtatc tgctctactt 79320

taggtacatg tttatcatgg cacattacat atacatactt acatactaga ctataatatt 79380

ttaattggtt atttaatctc tgtgtagaat ctctttccgt ttttatttct atacccatga 79440

cagtaccatt acggtctagt actaagcaat ctgttctaca cttaccttga caaattactt 79500

tctcattaaa tatacgtata tcatttatat cactagttat ctcatcgtct ttaaacatgt 79560

gttttttagt tcttataagg tctttaatat ctttttcata aaacttaact acatcattag 79620

acatttaacc tatctccttt tactctcttg caatactcaa caacactttc taaaggagta 79680

tatgttctat tccatttaac acttgttaga cctatgaaaa cacctgtgta ttgaccatgg 79740

actaccttag ttggttcttt ctgtgagtaa atagttctat ccttatctct ttcaaaaaaa 79800

ccttttccag ttttacctgt aactgtatct gttcctaaag gaataacatt agctacaaat 79860

atagggtctt ctacaacctt cttaggatat ttacgttgag gcatcatact cttaatcaaa 79920

ttactaagag gtgtacctcc aggtattgtc cgttggtaag gttctactct atatttcttc 79980

tgaggatttt ctttttcctc tctatcatgt atgtcataca caataacata ctcagacatt 80040

tcttcttcta tatgttcaat aaagtaataa ggggtatcat tgaaatagta tacaccctca 80100

ctaattctca ttgctaatga gctattatca aaagcactaa aattaaaatc catttttaag 80160

tcacctctat attaatatac catatatagt aaaaagagtc aagtaaagac ttgactctat 80220

aagaaatcct tttccatttt tctaatagta ggtacaatat cttctacgtc tgttaaatat 80280

tgtttcactg acatactagg agtttcttta ttgattagac ttactgaata tggttgacct 80340

ttaggatggt ctaccatacc atttgtcatt tctttcaaga atccatcatt gataacatta 80400

aataaatact tatcattacc tttatcaaat tgtattgtat aaatatttcc ttcatcatct 80460

tgagattcat gaatgttttg tattgtatca tttttaatta attgctcata aatatcattg 80520

aacttcatta ttatcctcct gtgtttgata ctgtctagcc tttctagctt ggaatattgt 80580

aagtacaaac gaccctacaa gaccgattat aacataaact agcatataaa taaagcttgt 80640

aataataaca ccgaatcctg attgcgcaga atacaaggaa tctctaaaaa taatactcag 80700

tataataaca ataacaccaa ctaagtaggg tactatcaca tgatagtact caaaattact 80760

tagtggaggt tgtccttgac ttaccatttt cttctctgta gtccataaat caaataatct 80820

aaagactata ttaaatgttg caaaagttat aaacgctaca cctataataa aaattgtatc 80880

catttattaa ccatccttaa tctaaaaata ctgttttaat atcaatatct ttattttctt 80940

tatttacaaa taataattgc tgtgaaggtt tagtttttga taaatgtagc tctttagcat 81000

aattattata tcccatcgga gatgaagcta caatatgcat tctattaaaa tcttcttgtt 81060

ttactgagaa atggtgtacg tgacctgtaa ttagtaaatc aatatggcta tctaagataa 81120

atttattaat atgattgcct ttacctttta atccatcacc gtggttaacg ataatagatt 81180

tacctccgaa attatcccta atagtataaa tatcttcacg attatcaata atatctatac 81240

cgtttaataa tccctgctct tggaatagca ataaagagtc tagtactaca taagcaatat 81300

tatcattata aatcttctga tttttgttac cttgcatacg gtcatgatta ccaccaataa 81360

taccaaatct taattctcct gtaactacat tagatagtac atttaagata tcaataagta 81420

atcgagtacc tttagagatt tgttctgcta aagtaaactc tgtttcaaat gcctggttaa 81480

cgtctctcat attaatatgt tctactaagt ctccaacaaa gtaaacagtt acattagaaa 81540

tctctctatc ctcaatctct ttaactgtcg tatcaataaa gtgatttagt cttgacttca 81600

atacttcaaa atcatattta ccattagtca tatcggatac aatacttcca atatggaaat 81660

ctgatagtaa tacaattaac tctgtatcat taatgttaac tttacttcca tgatgctcta 81720

ctaatgtttc cccttgtaat tctttaacca tatgttcttt aagttcatca aacataattt 81780

gagggtatgc ggtatgtctg ttgtgcttac gttgttctct caagtgagat agttcttttt 81840

ctttagctac tacataagta gagacttctt catgatagtt attaccttcg aatacttgat 81900

tatctgcaac atctttaagg tctcctaaag ttaccttacc tttaattgct ttattcttaa 81960

gctcatttag ataggcatct tgtctaatat cttcaacaat ttctttaagg tcgtctttac 82020

caattctatc taaaccaaac tctttagtaa ttttattaaa cttagatgtt acaattttac 82080

cttcatcttt aaataatact ccgataataa ctgcttgtac ttcttctatt gttttatagt 82140

tactcataac tgcttccttt ctctcttata aacttagtta tttgatttcc taaaatatta 82200

atgtgactat ttacgaatgt atgaaaaata ttagaatcat aatcatgttc tacattaaac 82260

atgctagcta ttgccttagc atccatacct ttaacgtaaa aagtcagtat atcattatta 82320

gaacgtgtct gtgaaccgat aataacaata ccatgctctt tatttgaaag taaactataa 82380

gctagaggta atttataatc aggcaagact tcatataata aatatacatt accattatta 82440

tagcactttt ctcttaccat gtcaacttct tttttataaa aactatttaa atcgaataca 82500

tcttcaaaat cttgaaagtt ataataccta gttaaatctt ttgcatccgt ttctacccat 82560

ttagggaaag ggtctttact taactcaact atctgctgta ataatgaata gtatttttta 82620

ttagaatcaa cagtgataaa agtatccgga agtaacccta tatcagaaac ataataaata 82680

tcattattct taatattgtt atcaatattt gaattagagt taataacctt attaaatgta 82740

atatcatcat agataaatcc tgctaaaaca gttggttcat cctgtattga tagtaatcct 82800

tcttcaaacg tatctacttt ctcaatatca aaaaaactat cgtactcata taaagtatct 82860

gactgaatat ggttagaatt tattataaca cccatacctt actcacctac tctttcgtat 82920

aagtaacctt taacgtctct ttcatcatct ttagaagcat tttcttgtcc gtattctttg 82980

actctgtaac catagatgtg caggtaatct tgaataacat tcatcatacc ttgaacattt 83040

aaattatgaa cagaaactga atcaaatttt actacaatgt actgttcttt gttttccatt 83100

aattttttta cttttgtgta atcatatgga tttctaaata atattgttgg aatatccatt 83160

actttccctc cttttcttaa ttcttatata gtgtatcata gatattataa attgtcaata 83220

aaaaaagacc aactttttag ttggtcttaa ttattatgct tcttctgaag gttcttcagc 83280

aggagtttta gttgtaaatt caggaacatc tactttttct gattcacctg aatcattagc 83340

aaatgctact ttaaaagctc ccttagcata agtttttccg gcttctaagc ctgtaataga 83400

tactgtagta gctccatcgt tattagctac ttgagagcct acaacttcac cattactata 83460

aacttttaat gtcttttcca ttctttattg tcctcctatg cagtaatatc tgcgctattt 83520

tcaccaggtt caactgttac atctgtaggt gcttcaggaa taggttcctt aactgttacc 83580

gcaacaatat cagtatgact accatcctca gtagttacag taatattagc tgttcctgca 83640

ctaactgctt caattaatcc ttctgatgtt acactagcaa cacctgattt gtctgattca 83700

aatgacacat ttttgttaga tgcttcacta ggcgtgatag ttgttgatag ttggtgagta 83760

tctccaactg taaggtctaa actatcaacg tcaagggtaa ctgaaataac tttaattgct 83820

tttgtcttaa actgagggac atcgaccttc tctgattctc ctgaatcatt tgagaatgat 83880

actttaaaag tacctttagg gtaatctgtg ttagcatcta aattctcaat cgtgattgtt 83940

gattttcctt ctgttttctc tacgctgttt agtaactcat tacctttgta taaatttaac 84000

ttagccaaat ttaccatcct ttatctatat tattctgctg atacgtctgc tccgtcttcc 84060

ttaggagtaa cttgaacgtt cttaggtgct gatggttctg aagtgtctcc ttgaccttct 84120

cctgaatcta cgcctttaac gtccttacga gcagggctag cagggaaatc cgcaattaca 84180

tcttcatgtg catgctctaa actatcttta acttcatctg catcattatc tgcagggtct 84240

acgatagata atacatcttc tttgttattt gcaacaaata cgttataaac accaaatttg 84300

aaacggtctt caacgaaagc taagaaagct tgaataagtt gtttaccttt atctaatcct 84360

ttaatatctt cagtacggat aaatgagtga ttataagggt ctactactgt atttactaca 84420

aaatggatat tgtctccatc agtgtaagca ataggtgatt tcttcattac atttaatgta 84480

cctttaccat ctgtttgtac tgggtataag aataactctt ctccctctga aactactttg 84540

tacgcatatt gtccttcgtg tgtacgagta taaccgtcac ggttaacttt aacttgtaaa 84600

tacttatctg ctactgtaac agattcttgt tgtaatacat cattaacatt ctttttagcc 84660

ataagcttaa tctccaatct ttattttagt aatctatttt aaagcattct ataagacata 84720

gaaaaaagcc ttccctatgc tttattattg aattctcgat aataatataa cacaaggaag 84780

gcactgtaac tagttttccc aaatatgatt ttcagtatta actctatgat tatttaattc 84840

tttctttgcg tagtactcat gatatgcatt tattttaaat ctaacataat ctcttaattc 84900

tgtcaattca cttgctactt cttttctttt catatcaaac ttctccgcta cttgagaaac 84960

tataaaggca tcatcttctc tttcaggatt aataagtaat tctttaagta gctcactttg 85020

aacttctgta aattctatat catcaaatac ataactcata atttgattat cctcaaagtc 85080

ctcatttaaa tcttctgtta aggactctac cgtataatct tttttaccga taatttcagt 85140

acgtttatac ttttcattct ttttaacata actattttga actctcaaag ttaatttagc 85200

tttaatatac cctggaaaat ctactttact tctaatatta tattctttaa ctagtttaat 85260

aaattgctca tcaatatatt ctcttaattc ttgtctttca aagtcattag ataatatacc 85320

tgcatatcta tgatacaacg accatctaag gtttttatac ctgttaagta aactatccaa 85380

gtctctttca aaatcaacag gaataccatc tatatctatt acatatctat taccattgtt 85440

agctttcttc ataaggatta gccccctcga agattattct tactttatgt gacttatcct 85500

taacatatat atcagagaaa tggatagttt taggcacttt aacctcttca ccttcatgtc 85560

taattaagga gtatcctcct gcccatctat ctgttacatg ttctatatac atcactgtag 85620

aatctgtgta agggaatcca ccttcttcaa tgataattga ttcacctttt tcttttactt 85680

cttcaaattt ctttgctata tctttataag gtaaaggaag aacatctgag gaatttgcat 85740

tccccaaaga ttcttttacc tgattcatgt ttttatctat taaattatcc atttattatt 85800

cagaatcctt ttcttgagat tctcctttta gttcttcctc ttgttccttt tgtttattat 85860

ctgtagccca ttctttatag taattttcaa gagcttgata ctcttctagt tgagttacaa 85920

ttactttatt attaagcaat ggtgagtaac cgttagggaa atgctctcca tatactctac 85980

tgaataattc taaatataac tctctattat cttttaatac aggaacccat tcagcatctc 86040

taagtttaat ctcttcacca ttcaatgtaa catagtttct ccatgcacct ttagtaatta 86100

aacctctttc aactgcttct ttatatacag tatgttctaa gtcaatacca tttaataata 86160

tagggtcatc ttctttacct accatataat cagatagtaa gtctgcttct gctttttgtc 86220

ccggtctcga taatttagat ttcttagttt caatacgcat gatatgacca tgatattcat 86280

ctttaccagt taattcactt ttttgtttta attgtgatgc tttatgaacc ttaatacgta 86340

aactagcact atgttcaaat gctctaccac ctgtagactt aatagggtca ccatacatac 86400

ctgcattcat atcgtcacgt gcttgattaa taacaattaa ccctgtattt gtatcattta 86460

atttaggtgt tactgcatta attacttttt gagtagctga tgctttaaga cccatttgtt 86520

tctcatcaat accgccgtca atctctttct gagttcgtgt agctcctagt gagtcccaaa 86580

taaacacaat aggtactcca ggtatctttt cattaaatgt gtcaatccag tactctaatt 86640

ctttacctac agcctctaca gataattcta ctgtattttt aagtctacct tctcctgatt 86700

gaatagagaa taattttgaa acatctactc caagttgttc catacgatta ttgtctgctg 86760

ttccttcaat atcaatccaa atggtaataa cacctaattg tgttgcaatc ctagacaaat 86820

gaactgcaaa tgttgattta ccactaccag ttaaaccata aacctctgtt aatctaccta 86880

acggtatacc tccacctaaa atatagtcat actgaggtac cattgtaggt ataacattct 86940

taatatctgc tctatttgta tctgataata atgttaatcc taattcttta cctaaatcaa 87000

ttgtatttaa atctgttaaa tctacttctt tacctttttt tgctcttgcc atttaatatc 87060

ctcctagtaa ttatatctca aataaaagag tgctctaagg cactctatat cttataggtt 87120

atctaagtct agaccaccta atacatcgtc aatactttgt gtttgttttt gttttggagg 87180

ctcctgagta gtttgttgtg gttcaggtgc ttgttgtgtg ttttgaggtt gtgtctcaaa 87240

aggtacttgt gaatcatcaa cactgtttac atcaaagtca ttaaaaggat ttgaatccgg 87300

ttgtgattgt tgttgagcta agttatccca atcaactgaa cctgtattat taggttgttg 87360

tcctgaaggt gttccttgac caaattgagt attactaatt ggctgttggt taccttgtaa 87420

ctgctgtcct tgaccattat tttgaccttg ttgagtatac tgacctactt gaccttgttg 87480

tggttggtta ggttgacctc caatattact tggcatctga ctatcaatat cttgttgagt 87540

aggagcttgt ttaggttctg aaggttcttc acctaataca ttagactcac ggttaaattt 87600

aaagttatca tgacttaact ctgtgttatt aacgttattg attaagaagt taacaaagtt 87660

agggttttgt tcttctgttg gttttgctaa ttgttctaag tctgataatt gttgttccca 87720

tccttgaggt aatgcaccta aaggagcatt aggataaaca cttactttcc atgacatttc 87780

acctttttta gctttagcaa tattaactaa gaatgcttca tttgctgaaa taaaactatg 87840

aggtgcatta ggtgacggtt ttaacatagt atccttaagg tttgctacca attctttata 87900

tcctgtgttt gataactcca taggttgaat cattacattt ccttgctcat ctgttttagg 87960

ttgaatttgt ccgttattat taaagtattc aataacatga atgtaataac gtactccagg 88020

tttattaggg aatctactaa attgaacgcc atttgttatc cagtcctgaa tgtaagggtc 88080

aaggacagat gaacctggtt ctgcaggtaa tgttagtcct gtgaatcctt gagaaccatc 88140

tttcttagag tagttaatac ctgttgttct aaattcttta aagaaaacat ttgttccttg 88200

tgcaggaggt aatactcgta caaaagcatt ttctttagta actttatttc catttgcatc 88260

tttaatatta cctaatcgta atacagggtt tttaggttta tagctctcta cattgttgtt 88320

aaaagaactg ttattacttt ctaatttgct tgcctcattg ttaataaatt gattgaaatc 88380

cataattatc ttttctcctt ttaattaatt tacttattta ttataacata gttttttata 88440

tttgtcaatt atattcttta ctttcttttt taatgtgaaa atgggttagt aattccacca 88500

tttttatttg tttgtcgtaa ctctgcacct aattgaatca tcatatctct tcttgtttca 88560

aaagctttta caacatattg aagttgttta actttataat tccaagcctc aacaacttta 88620

agttgttgtt gataggaatc atgaatatac acactagact caatcatatc tttagtaggc 88680

tttttacctt gttgttcata ctcttgtcta attgtgagat taagcttact accaattgtt 88740

tctaaatttc tttgctccat ttcagaatat gctctgacct tctctaatat agaagcccaa 88800

aaagcgtact tagctgattg ttgcataaaa tcttcttgta ttgtatattc tgtaatcttt 88860

aattcatctg taatatcaaa ctcttgtgag ttcccatttc tatctttaat agtaaaattt 88920

gtaaaatcta gggaatcaat gtgtatctcc atttatcagt tcctcctttt aaatattaac 88980

tgaaatacta cttaacggta aagctaaagg atattcttct tcaagacttg ctaattcttc 89040

tgttacttct ccagtagatg actctgttgg taaaacggta gagccatgtt tattttcttc 89100

tctattatag aatgtaacaa caatattcat ttcacctaat tcatcctggt acatacttgt 89160

tataatccat tcatccatag aacgattaaa tctaatctct cctgtattca ctcttttttc 89220

ctccttatac ttattaccta tatagtataa cataagtgaa acatattgtc aatactttta 89280

ttaaatttct tgatagatat gtttttcact tttaacaaca ttagtagctt tttcaaattg 89340

ttcatcagtt aatttacctg actctttata atcttctaaa gtctgtaaat tcatcatgta 89400

cttaatataa ccttggtaag aattaaatgt atcaatttct tcttcatcgt attcaaccat 89460

atcattataa tttaaaccaa tttccatatc agcttctatt gggtattgta cttctttccc 89520

gtcaatttct gcttttaaga aatcaaacgg taaattctcc ataatatgaa ttgtcacttt 89580

agccataatc ttagcctctt caggagggca atcaattaag atactatcgt gtactgtagc 89640

aataactttt gatttcaagt tacgagactg aataaaatca tctaagtaag ttaaagacat 89700

atttgttagg aaactacctg aaccctggat aatagtatta aatgattgtc ttagaccttc 89760

attttttatc tttttatctg ttgattgtgc tgaacgtata aatcttctat gaccgtgcat 89820

tgtatcaaca taaccatatt gttgcacaaa ttcatgtaca ttgtcaatag aagtttttac 89880

actaggtttt gtttggaagt acttttcaaa tatttcttct gcttcactta cttccatatt 89940

atttttacct gcaaatgaga atggtgattc tccaaaggct agcccaaagt taactgcttt 90000

agttgcttgt ctctcttctt tagttacttc ttctactggt ttattataaa caatacttgc 90060

cgtagcctta tgaatatcct caccatttaa gaatgattgt agcatatcag ggtctttagt 90120

aaataatcca ataatacgca tctctagggc actatagtcg gctcctagta gtactccatt 90180

ttcaaatcta gaaacaaatg aacgtttaat tggatgttta taatcaaact tgtttacatc 90240

tgatgtatga gcaggtaagt tttgtaagtt aggattacta ctacttaatc ttgatgtctc 90300

tgtacctgtc tcagaaaaag aaccatgtaa tgttcgtttt tgtttattaa tcatattagg 90360

taatttctta gtaaatgagt tacgttttgt ctgcatacta gcatgatatt taagtaattc 90420

aagaagttct ttaacatcat cttttaattc taagttatct aatgcataac caatagcttt 90480

cttatctgtt ttataatcct gccaagtaag gtctgcttct ttaacattag cgttaaatgg 90540

tttttcttta acatactctt tatcataagg taactgaatt cctaaaatat cataaattac 90600

tctaccttta tggtctcctg agctaggaga aaacatccat ttaccatctt taaacttatc 90660

tctatactga tgaatctctt tgtctctatc ttttggcttt ttctcatgct cttctaacgc 90720

catttggtaa aggttatatt gatactcttc aaattcttta acagcccaat gttctctcat 90780

agtagcgtgg ttcttagcca tctcagattc gtatgactca tcattttctt tcatgtaatc 90840

taaatcacag tataaaccat ttgattcaat tctagctaat gatttagtaa gtcttgggta 90900

attaacttct aataaatgca ttgacttagg tctatcttgc tctttaagtt tcttaacaac 90960

atcacaatga attcttctac atacatcagt gtctcctgat gcgtacgggt gcattaactc 91020

aatagggaac caatcatagt taaagttacc tccatcaata tcattaatta catcttttac 91080

atctctatac ttgttaatta aatcaattgc agtattcagt gtgtaagatt tagcgtaatc 91140

agataatccc atatattcag gtgattgctc tgcaatacta ataaattcat cgttgttcat 91200

aactaaattc atgtttacaa tttcaggtga aagacctaac tgaatgtatt ttttctctaa 91260

ttcactaact ctaaatttct tctcagtatc atctaattct actattgttt catttaattt 91320

agtctctaac cactctttgt attcaggagc tttaacatca tactctttct tagcaatttt 91380

tttattttct ttctgtattt ctttaatttt atcagagaaa aatcttaata acttagtaac 91440

aaaccataat ttaaaatctt ctagtggttt atcataacct ccgacatctg ttacctcgta 91500

agctaagtca gataatctca aagattcttt aacttcttgt gtgacagcta aataccaacc 91560

tactttagta tcttgaatgt tctcaaaatc tttaaagttc tcagtactca ttaaaaattt 91620

aatatcatat ttaccattat gtgccacctt aatatcttct ttactagcta accaattctt 91680

aagtaattct agtacttcat caatatcttc ttgaccattt tcccaattaa agtccgattt 91740

gtataaaggt atagtcacac cttgaccatt tctccatgat agtgataata ctaaaggttt 91800

acttccttcc ttatcaggct ttaatgagtt agtctctaag tcccatgcgg tgatatctac 91860

accatcataa ttatcattct ttacttcctt attaaatatt tcccttactc tttcaatgtt 91920

atctactaat tcgtaagata cttctttagg tttaaatgct tcttcacctt gttctacaaa 91980

ttttcctact gtttgtaaat ctgatacaac atgacgttca ctatttttat ttacattcgt 92040

atattcaata ctatacgtag gtagtaccca tacatcatga gaagatattt cattttcaat 92100

tgttacttta cttggtactc ctcttacttt acctatagct gtaacattta ataagtattt 92160

aacacctagt ttaccaacag gaattatcat atcataggag ttatcaataa taatgttatt 92220

cattctttca taatatggtt ttacttctgc ttgtttaaca tcttggtatt taataatttt 92280

accataatca tttctaatag gtgtaggtac tgcgttatag agaaaatcaa tatcatagtc 92340

tctgtttgtt ttatctctct ttaagttaga acacttctca agtaatttct taagtgtttt 92400

tccattaggt gtatttaata cattagattt cacactccca tctttactta cagaaaaatg 92460

ttcttctcta atgtggtcaa ataagattaa tactttcatt taattcctct ccttttttaa 92520

cttaataata ctataccata tattttaaat cttgtcaata aaaaaagaag agaattattt 92580

ctcttcttca ttaagtctat caataacctc ttctaattct ttaagtggtt taaattttat 92640

agcttttcta ttaggtaaat ggaaatattc tttattcagc ccatcccaag cttttttacc 92700

ttttctttca ataacttcta tttgcataaa tttatgattc tttactttag tatacccttc 92760

ttctagtaag tcagaaatag catctgtctc tgcacttagt atctcttcta catcttgtat 92820

ataataacct gtgttctctg atatcttcct tgctatatct cttctatttg ctgtactcat 92880

ataatcacct tatatcttaa tctttcgttg ttgttttagt ttcgtaatta cattgtctac 92940

atcttcaaaa tcacgactac ttgttaagat atagatatac attttccata ctgataggga 93000

cactctaatt ttatctgcaa aatcaacttt atattcaggt tttaaatcat aagcttcacc 93060

atcaaagaaa taaaattttt cttgtttttc atctacttct aatatacgag gaaatgataa 93120

atgaacttca tctacatttg ttttaatatc atgtaaactt ctaataatat cataaggatt 93180

tacataagga aataccacag aaatatcaat aataactttt gttcccataa atgataaagc 93240

tatattatct acttcttttg cagtaaagcc gttacgcata cgataaacaa cttctgaatc 93300

agggtgacta gataagtctt ttaaaacata ataaggtaat actgaatcag tatagtatgt 93360

aactttacca ccatgtttac atactttctc tatgagccta tcattattgt atttctcatt 93420

aaataacata taatgatttt catctctcat aatatatcgt ctagatttag atattagttc 93480

atccatattc ttttcaattt cttcttcatt accgtgtgtg actttaatag cctcattaat 93540

tttatcttta tcttttgtgt gtatattaaa atatctatca ctattatata tcgttaatgt 93600

tttattcttt gcgatatgtc atcttccttt cattaaaaaa atcctactac cttgtactat 93660

tatagtatag cacagatagt aggaaattcc aaattaaagt gactctaatt cttgtaattg 93720

cattggtgta acttggtctt catatttttt aactaactga ccatctttat acccaattaa 93780

tactggtgta ctcatgatat caaataactt aacagccttt tctctatcta catcttctgc 93840

atctaaatta agtgttaaaa taggtttctt aatatcccct gaatcctgga acatagggat 93900

tacacttttt aagatttcac acttaccaca attatcttgt gtaatcatga caataacatc 93960

tttcttttgt ctaattgctg tatttaaatc tactaaatta ttcatttttt ccatcttctg 94020

agtcatcctt ttctaatgtt ttaataattt cgggaaatga gttaactgct gtagcttttt 94080

gtgcttcata accttctgat aaatcaatga gttttaactg tcctgagcca taagataaag 94140

tatacatagt catatcatgc ataaactcta actgaaaaga cttagacgta tcatcagctt 94200

tggcatcttt agagttacta aataacttca tagggtcatt gatatcactc acacatgaaa 94260

actcatcatg ttttttaaat aatatttctg ttttacttaa taaatccatt gcttcttttc 94320

tatccataat atcttttctc ctttttatag gaattcctta gcagtactat actctttact 94380

atctaagaat gagaaatctt catctttaat atctttaatg tttgtagatt tttcgtagtc 94440

tgctttaccg ctaaagaaat caacatttct taatctgtct acattagttt gattttctac 94500

aatagggtta taaggttcag ggttaaaata gtcttctctt ccaaggtttt gaagagctct 94560

attaaagtta tactgaacgt agttaattac atcttcactt aactctagcg ggtcatatag 94620

actatgtgta tacgctactt cattagtata aagaatattt aataattcat aagtttctct 94680

atctgctaat tttttttctt cttctgttaa aagattgtaa tcatattgag ctgttaatcc 94740

tactgctgaa ccgtgataag cttcatcttg ggtaatttta taaataatag ctcctgattg 94800

tgtcatctgc cctcttcctg caagaagtaa agggtaataa aatcctgagt aaaataatgc 94860

actttctaaa aaggcactag ctactttagc catatatcta tcaaatacag taggattagg 94920

ttttaataat tggtcataat aatatccaat aaactgagct tttactttta aaaagtcatt 94980

ttcttcaacc caagtatcta gtaaataact tgtttcttta ttacttaata gtgttgtaaa 95040

gatatgacta taagatttag catgtatctc ttccatacca cccataaacg caaatacagc 95100

ttggtatttg ggtcttggtt catgataaga tactaagttc ataccttccc ctccttgctt 95160

cgtatcgagc cctgtgagcc ctgcaaggac tttcttataa gtgttctttt cactctcagt 95220

taaactgttc caagaactta aatctcttga tacatcaaac tcttcaggag tccaaaattg 95280

ggagattcct tgctcccaat aaatatcaga catcccgtca tcttcaatat tccaatttgt 95340

tgcctttaat actgcatttt tattatgttg ttttactttt tgtgtaatat ccatatctat 95400

ttcctttcta aaccgaacat gtttcacatt caataacaga aagtttacgt gaacgtgtat 95460

aatataatga ttttaatcct tgttcccaag catagtaata taatgatact aacttattag 95520

tcggtatttc tgattctaca taaagtatag tagatactgc ttggtctgta tgtttttgca 95580

ctactgcact tgtattaata atacgtttat tatcaatctt ataagctgtt tctccttcat 95640

agaaaaactg agttatagga cttaaataag gcattggata gtatgtttcc atgtctccat 95700

attgtctgtt ctctatagca ctaggtacag gcataattga gcttgtagca ttctgtacat 95760

atgatataga ttgagtaggt gcttctgctt ttctataccc attatacagt ccatatactt 95820

taactgcttt agctaattca tcccaatcat cttgtgttgg aatatagacc ttgcttagga 95880

ctttcttagc tttatctgtt ttaggttggt tagactctct aatatatctt acaaagtatt 95940

cacctgtagc ataatcagac ttttcaaacc ctttaaatgg ttttcctgtt tctttagcca 96000

ttaacataga tgtcttaata gactggaagt taatagcact atataaactg tttactaggt 96060

ctagagcttc cctagaacca taactaatca tattcttagc taaaagtcca tgtaaattca 96120

ttgaacctaa accgatagct ctaatgtcat catttgcttt ttgaacacta ggtagatatg 96180

gtaaatccat aatatcagta acttttgtta gtgctcttgt accaatatct acggactcat 96240

ttaataaacc tttttcaacc acattaacta agttaagtga acctaatgta caaacaacat 96300

ctctattaat aacattctga ttactatagg aatatggata aatttctgat acctcttgta 96360

attgtgaaat ttctgtacat aagttactca ttttaacttt acctagattt ttaagagggt 96420

gattgtcatt agcattatca atataaaata cataagggta acctgattct aattgtgttt 96480

tagcaatatc tgtcatgact tctctagcat catgttttac tttaccaatt ttatcattat 96540

ttaataattc ttcgtaccac tcatcaatgt ttaattcatc taaatattta ccatatactt 96600

tgttaatatc gtaagtgtca aacgtgtaga aaggtttacc ttctttaact aattccataa 96660

acttattagg tattgtaaca cctatagata gtttatcaag tctaacagat tcactagcat 96720

ttattttctt agaacttaat aaatccaaaa tatcattatg gaatatatta aggtagactg 96780

caccactacc ttgtcgaacc ccgccttggt catatatact tactttatgt tcaatagctt 96840

tagcaaaagc aataacacct ttacttgaat taggtctatt acgtactgga gcacctttcg 96900

gtctaattct agttaaatca attgcaactc cgccaccatt agaactagca ttagctacac 96960

tatcctcaac aaaatttaag gactcaattg aatcatctac tacaaataga taacatgaac 97020

ttagttcacc tctattagct tgccctgagt tattatacgt aggtgtagat ggttgaaatg 97080

tttgctccat aagttgtact aataactctc tagcctttac ataatcattt tgaaataaat 97140

ataaagctac tcgaacgtta tgttgctcat agtcttcaac aaaaataggt tttttattct 97200

cgtcatactg ttttgatgcg taagactcat aaaacttttg ccctgccata aaactaggaa 97260

atttaaaatt aaaactgtaa gctagcttag tcatttcatc aataatagta tcaggaactt 97320

tatcaaatat tttatcatat gttccttctt ttgtcaatac tctaattctt tctacttcat 97380

tattaaactt tcttgtatta tcttcaatat attttaaata ctcatctaaa gcttcttggt 97440

ctttatagag tttatttttt ccattgtcat ctaattgagt tatttcatta tttaactcaa 97500

tccattttcc atatgttgcc atatatctct catcctttac gctactttat atttattact 97560

aaagtgttct aatattggtt gtatctcgtt aaagtgtcct gattgctcta ctttagcaat 97620

taaaggaatc ttaaacatct ctgaaatctt tttacttgct ccacagaaat tagagcccca 97680

ttgtttattt cctgtaccaa tgacttcctt agggctccca ttaatttcta ggaatctttc 97740

tatttgttta ggtattgcac ccttcatata agtcggtgtt attagtataa aaggtttatt 97800

tattttctct tttccactct taaccctaaa cgtttcaaat gagttattaa tattaatttt 97860

gtttactagt ctttctgtct gccctgtacc tgaaaaatat acaattaatg gtctttcttt 97920

acttgtcata cctatcttac ccttccttat atatcattaa gaaggtcatt aatcaattca 97980

tcttcatgct tacttacctc agataaatta gactcaacct tcttgtatgg ttttatatct 98040

tttttgtcgt agcaagatat aatataagaa ggtgtaaatg aggtcaaccc attcatggta 98100

gttataagga catcataaaa aaatttatct tttctaatat tatcaataat aaaatctgtt 98160

ctcataacag gatattcatt atttcttaag tcctcatata ctttctcatc ataaggtaaa 98220

gcaacgtaac tctgagctct gtttctagta aatattaagc aaggtgtctt attcaccttg 98280

ctactatcat taattacttg ttcccaccat gtatgtggtt ctctattgtt taaaagaacg 98340

ttatctatag tccattcttc tctatgttta cattctacta ctaaaggaaa attagcttcc 98400

tgaggtacta ctatgtctcc gacagcatta ttatctttac cccaagaggc accacctgat 98460

tgaggagacc tattaaattg gtatccccac caagcagtta attctttagc tattttacgt 98520

tcgaatgtat ctcctttttt cttactgttc gtcatttttc ttcaactgaa ttactttttc 98580

attgctgtca gattcagttt ctttttgtgt ctcttcttgt tgtttttgac gtaattcttc 98640

ttgcttctct tttaaataag cttctcgttt ctcatttaac ttatctttag cttttccaac 98700

ttgttcttga gttactccta actcatcttc cataacaatc atcattaagt caattgcgtt 98760

aaatgcgtca ttaaaatctc ctgacatctc ttgaactgta acagcattaa tgactttagc 98820

aatacgagtt gcttcttcta aagaaacact gcgtgaacca attctatcta gttgtttagc 98880

tgttttttca tcaacttgtt taccatcaac agttccttgg actacagctt caatccattt 98940

ctccatgtct gcttgactaa taccttttgt gtgtgtgcta atattttttt ccattattct 99000

tgttctcctt tttcttcttc atttttaggt tcttcttgtg atttatcaat tgattcttgg 99060

atgtaatcta gtacatcttc attaataagt tctgctttat ataaagcgtc aattaaaata 99120

tttaaattta actcattgtt gtattccaag tctgtcattt gctcattaac acctaacata 99180

aggttagtaa tagctttagc tacatctgtt aatgttgctg tttcttcttt acccatttct 99240

tctaattgat gttgtcgttg ctcattacca actcttaaag ctgtaatgta ttcatctaca 99300

aattctaaaa tatctttttt attatttgac attatttttt ctcctttatt aattacttaa 99360

ttattagtat aacataaaac ttctctaagg tcaagcctaa agtttaagtt tttgttgtat 99420

tttgtaactc tgtatacttt caggtgttac tagtactcgg ttctgtttta atagctcaaa 99480

agctttcctt gttcccatat catttgcatc ttcatcacca tgcggtacaa aataaacttt 99540

gttaaagtgc gtttttagct tataagctaa atctatatta ttatctaacg catcagtatc 99600

taacataata tatattgatg tatctatgct aacataatca attatttttt ttatttgatt 99660

ttctgttact tgtttaccta gtgttgctac tccatattca tgaaaggtta aagcatcaaa 99720

gacaccctcg gttataacta agaatttttt ctttcttgct atatttaaat taaatatgac 99780

atcttttctt cctacttcat cttgtttagc aggggcattg atagatttaa tataagggtt 99840

cttctctata cttcgtgtat tccagtactg gtagtttcca tcattatcat acgtaaaaaa 99900

tataatgcta ttccttaatg agacttttga ttctccatca actttataga agcacccatt 99960

aataacataa cctatattat tattaattat ttgttctaaa gttatacctc tgccttttaa 100020

gtattttaaa aaaggtataa tctctttatt atttaagtta tcttttaata acttatatcc 100080

ctcaggtaat ctaggaggtt taagattagt gtttccttta tcttggtgta cgcctctaag 100140

cattaatata agtttctctg attctgttaa atccttatta ttagttgtaa gtaaaggggc 100200

tctctctata tctatattct tagactctaa taaatcaaaa gcttgtttac ctgtaatgtt 100260

ataataagtc ttcataaatg taataggatt acctgattca tcacattttt tacagtgata 100320

ctgaccatta ctagagtcta aagcttgctt aacatagaac ttataacttt tttctccgca 100380

aaacggacaa cagtatctta gttcacctat agtattttct tttggttctc ctaattcttg 100440

ggttaaaaag tcttcaaacc tcataatatc cttccttact atttactaat ttttttcatc 100500

aatagtaaca tattataaat ctgccctagg tcactataag ttaaatctac cgaaaaatca 100560

ctctctttaa gtgcttcatt taattgtgac tcttccgatt tcatgttaaa aattctcaat 100620

tcttcttctg ttttaccttc taggtatcta gtaacaacaa tagttttaag actttggaat 100680

ataactccaa tgtctctaaa cccttgttct acaatattag catcatattc ttctgttata 100740

accggtaaac catattgttt agctttctca ataatcttta aattatcttt acgtactgca 100800

ggattatacc aatatcctat tttacatcta tggttattat ctctaataat atcttggtta 100860

ttatcctcta agccataaga agaaggtaag gtagaacgta gcctttttaa ttctaaatac 100920

tctccatttc tacttaactt atattcataa gcattactta aaggatacca atcaactaag 100980

ttagcagtag gtacattatc tatcgttttg tgctccttat tacgatgaaa cacattaagt 101040

gttgcattat ctctatctaa aatctttaat ttcattattt ttcctccagt tttgatactc 101100

cattttcttt tactatttta attgtttgct caaacagtgg tttaagttct gtattatggg 101160

taattacaaa tatagttcct actgtattaa gtctatcctt taataactta attacatttt 101220

cacaccctat agtgtctaac ccatcaaaac attcatcata aagtgcaata tttgtagata 101280

tctcatcctt actcataatt aaatcctgga ttgcaaaact aattgctaag tcaatacgtt 101340

tttgttctcc tgctgagttt gatttatatg agcctccacc tttactattt ttaacaataa 101400

catcaaactt atcttttagt tctcctttag cattcttcac ttgagtttga aactctattt 101460

caatatctga acctgataaa gtttgaagat attcattggc tttctcattt aagaagggtg 101520

taataaagtc taacactact gaacgtatac ctttattact aaaagcgtct acagcttgtt 101580

gatacttacc tttcttagtt tctaattgta ctacattctc ttcatgtttg tcaatagatt 101640

cttgtaattc ttttttatct tcttcatgtt tctttttact tggttcttct ctttctaatc 101700

cttgaacaga ttgatgttga tatttcactt ttaaataagt tttattattc tctaatgaac 101760

taatatctga tttaatcttg gaagcctctt gaatgatatt attaatccta gtgttgtgct 101820

ctcgttcttg ttggtcttct tgagacattt tgtctttaat ttcttgtgat ttattattta 101880

ctttatcaat agcttcttta tacgtatctt taatttctag tacactagta atctcttgtt 101940

tcttattctc aatctgatta ttaatatttt cctgctcttt taatttatga gaattatcta 102000

taggagaacc acaaaccgga caatggtcat ttgtatctaa ttgatttaag ctattactta 102060

atgtagctat ttcagatttt agtacctgct ctttagtatt atactctgca tatacttgat 102120

ttagttcagt taatttatta gaaagtaaat ctaggctttg agaagcttta ttatactcct 102180

ctgagtgctt aaattcatct tcaggtattg ttggtatctg tgaagttaat tgctctattt 102240

gagcttctat ttgcttctcc tcttgctccc ttaatttatc tttttcctta ttactatttt 102300

ctatttgagc caattgttct ttatattgat tatactgttc aatttccttt tgaaagtatt 102360

catcttttgt agataactga taccctagtt tatctatttc gtgtcttaga ttattctgtt 102420

gttcttctac ttctttaact ttctcttttg ctacatcttg tgcttgttta tatacgtctg 102480

tcttggtaat agattcaaga atttcttttt tccctttatc tgttgcttga gagaacatag 102540

gaatatctcc ttgaccatac atgatagcat taacatacgt attaaactca ataccaaata 102600

aatcctgtat ctgtttatca gtaacatcat ttgttgagcc tgtaatttct ttatcattaa 102660

caaataattt tactttgttc ttattctctt tatctttacg ataacgttca attaaatagc 102720

tatcttctcc aatatcaaac ttaagtttaa cagatgtatt tttcttctca atattattta 102780

ctacatcatc tgcttttaat cctttttcag ttttaccata taaagcataa gtaatggcag 102840

atattaaagt tgacttaccg gaaccatttg aatggaatga ctcattggat ttattttcac 102900

cttcaataag tattaaacct ctattatcta actctagtgt agcctcttta attgctaaaa 102960

aattattaac ttctacttgt ttaaatacga ccattactta tctcctttcg taaccctaaa 103020

gaaacaatga tgagaaccat aatcaatcca ataatattca tcatgttcta cagttctatt 103080

atagtaatgt ttttcaaaat tactatcgtc aaaaatcttt ttcattttag ataaaccatc 103140

ttctaaacct tcaacattac aaatgttctc tattactctg tctgaatctt gaaattgtaa 103200

aataggctta cttttatcta agtctttact tactacatta ctgatgtcta atgtattaat 103260

tttaggtttc atatctttaa acactttcca taaatcttct tttatccgtt cccaatcttc 103320

tgctaaattt tcttcatctg acttgtttag atattcttta tattttctca ctataaaacc 103380

tccttcaaac attcaagtat ttcttgttct gattcaggtg agtattgctt agcataagtg 103440

ctagcaattg ttagagggtc atcagataca tcactatcta tacgtttctc tacagtatat 103500

tcttttttaa tttgaacttc aacgttttta tccttcatac tatcatccat ctctaaaact 103560

ttagcatggt ttgaggtacc aataactcta ataaaattat cttcctctat taattgttca 103620

aagttttcag gtatatcttc tccctggata gtaataaatc tacgtgtatg aattggaata 103680

aattctgtgg tcatcttttc tgtatctatt aggtgaacac cgttagcttc ttgttcatca 103740

gaaaatgatt gttgcattaa tgaaccccca taaaaatggt ttgggttttg gaaatattgt 103800

ctacgatgat aatgacctag taaaataaaa tcatatttat caggtaataa gtcctgatat 103860

ccaaaggctc cttctaatct atgggaacct ttaccagtta atgacccttc tacacctaga 103920

tgacctacta gtatattaac catatcttta tcatagttac tgttaatgta ggtttttatt 103980

tcttctgttt catctccata agctgtaaat acaatattaa cattatcttt taaaattgta 104040

tttaatgact ttattacact tacattagat aaatactcaa atgtatctat acttgaatcg 104100

gtatataatg aattagttgt agcatcatga ttacctcgaa gtaataatac agggacatct 104160

ttatttttag caaatgtact aaatactttg ttatatactc tagtatctac tgaattacgt 104220

ttgtggaata aatctcctcc gaaaataaca ttagcctttt ctttcctagc aatatcaaaa 104280

actttctgta atgcttctat ttgctcttta aatctatcat ttacaaattc atcatcaggt 104340

tttgcatagt ttgtaaataa atgaaaatga ctatctgtaa aaaatacaaa cttcattaag 104400

ctcttcctcc ttggtataat tcataagtat actttcctgt tctatcaatt acttctcctg 104460

cattaataaa tcctagttca tttaagatat cagctacttt aatattatct ttatttatag 104520

ttaaacatat atgaggttct tcttttatta taagagaaac catctgttta aaatatccat 104580

taccatgttc cccaaccata acaaaccttt tcataaatac taattcatta tacttttcta 104640

ttgcacatga acataataaa ttttcactat catcaaacat accatataga tttaaatcat 104700

aataatcatt ataaacatca tctcttgttg agtagtaact aggtattagc ttgctctcaa 104760

tcaaaaggtg atatttgttt ggattattac taggtaatat ttgtttaatt ttcatattaa 104820

tctctccttt tattctttat aaaataatta taccacctaa catataaaat gtcaagtggt 104880

atatagtaaa aatttaaact cccggtaatc ctccgaatgt gttatttata gcttctattt 104940

tattatcttt attttggaat ttacttgtat cttcttttcc attgtcagat aatagctgta 105000

tatgttcttg tttttcttcc ggtgtttcat ctcttacaat catcttagtt ggttctacct 105060

taagattaac aaatcgttcc cctgtattag aactgttacg aattttatct aagtataatc 105120

ttaggaaacc actcttgaat tcttcatctt tttggtttac tgctagagaa acctcaacag 105180

cattgacaat cttacgagaa ccttctacat gctcacttgt aataacatct gaaccataag 105240

ctccacggtt ggtctgagct aaagtccaac atacaaaccc atattgctgt gataatctac 105300

gaatatcttc aaacagtttt cctcctgcat ctgattctga gtgatattta gcatatggat 105360

ttctcatcaa gtgagggtag tcaataataa caacatcaat atgcttatct ttcttgattg 105420

ttgtattaac aataatttgc tctagttggt ttggtgtaac ttctcctggc atatgtttag 105480

aaatataaaa gtcacctagt aactttctat tcttctgata atggttttgt attgcatcat 105540

aaactttact gtttaaagac atgtcttggt ttacaatttg gcttttttct gctcctgcca 105600

tttgttgctc tgcacgtaat accattctat ccattttttc ctctaatgca atatataaaa 105660

cacttaatcc actcttcaca taatttttag ctaagttaga agccattaat gatttacctc 105720

taccagttgg agctataatt aatccaacct ctcctcttgc tataccacct tcaatatgat 105780

tatcaataga gttaaatcct gtagagaatt tatttgtagc taaattactt aataattctt 105840

gtttcttttc agtgtcttcg aagaaatcaa taaattctcc gttacctcct gaaatatcac 105900

ttacttctat ttgttttaat ttatctacaa gttcatgtaa attgtctgag tcttcttgtt 105960

tattttctgc aataaactta actaatactt ccttagacat ttctgtttta atatatttct 106020

ctatttcata attaacagat tcatcttttt cattaacatc tactttatat aaatcttcta 106080

agtaagtcaa tgtcttggtt actttttctt catcttcctt attatttccc ataagctgtt 106140

ctactttaat tgctaatgct tcattagaca tcttatcgga aatatgtgct gttcttttta 106200

tagctgtaaa taagtaaccc atatcttcag attctgctga aaataggttc ttaggtaagt 106260

tatctagtac ttctcttgca aaatgtatat ccttcattga tttatgaagg ataagctcct 106320

ttatcttctt actcatagtt attaacctct tcctctacta aatgtataag tttattataa 106380

tccaacatac cctcttttgt caatggtatt ttatcaatta aattatattt ctctacaata 106440

tattttactt ctcttaagtt tacttcatat cctagataat caccaaacat tcttagtata 106500

taataagttg aagtatgttg tctcatatat aggtactcat taccattctg ctctaagttt 106560

tgtatatcca taccacttag tgaatcttta ttcactatac ctcctagact taatggtaat 106620

aaatcttctc tcttgattcc tctgtttaac tgtttattat aaactatatg ctctttctgc 106680

atagtaaaca ttgataatct tgcagatggt gatatgcttg ttggggagta atcattaatt 106740

atgcattgtt ttacatattt attaataata cttttctctt ctgtaggtaa gttcttaatt 106800

tcttcttcaa tcatagaatt atattgtagt aaaataatat gtttcatatc tccaaatagt 106860

ccataagaag cattctcaag gtctaaggct tgctcaaaca tagtatcaat atcatgtaaa 106920

gtagaattta atcctgtagt atataattga tgtaaatgta atacagcagg gttcttaaca 106980

taatctgatg aattaataat actatctgta tcccctaagt gtctatttgt actattaaca 107040

ccttttttta ttcctttaat ataattatta tagtttttaa aatacttatc tgaactaaag 107100

aagttaggag aaggtattgg attaggttgc ataccattct cataataaaa tgttacattc 107160

ttaaatacgt tttgcatata tttaaataca ttaatatttt tatcttttat ttttaaataa 107220

aaattataga aagtattaaa cattttacta ccgaagaaat tatcactaac actatttcta 107280

ggtaaatttt gaatcatttt atttctatag tattcagaca tatgttgatg gtaatcatat 107340

tcatctttag ttgttgcctt actcattgct ttaagatggc ttgtatgttt agcatccata 107400

tgagaaatag catattggtc atataactta catagaatgt atgctttata aaatccttcc 107460

gggtcataag acatattaaa tatatcttta gaaggataag gaagaccttc tgaatgaaag 107520

ttcatatctg ctataattct tctcttttca tctttaatag cattatactg tgccatttct 107580

aacttagtac gtctacgctg ttctttcctc tcgtatctat aagaaggaaa gtgcttttct 107640

cttaatgctt tagcatactg taaatcagaa gtaatgatat tatctgtaga attattaaat 107700

tctttcaatt cttctgtgtc atattcccta actaaagtta ttattactcc accatttttt 107760

cctcttttag aaacagtaac aactttattt tcttcttcta actttttaag gttattggat 107820

aaagcagaga tggaaacgcc tagagaatct gctaactctt tcttagtaga aatatgaaat 107880

ttattttctg tagcattttc ttctagatat tggtataact ttcgttgtgt tcttgttgcc 107940

atctcttacc ctcctatttt cctaagtctt ttatttcaaa atcttcctct tcataaattt 108000

tcctacgctc attagcatga gtatataaaa atctatttgt catatcatta aaatcaaata 108060

tttgtgttgt attatcgtct ttcttcttac gtaacgcacg accaatacgt tgtaatgttt 108120

gtcttaacga tttacctcct gcacctaata ttagcgcatt aataccggat atatctacac 108180

cctcatcaat aagtgatgta gctatcatta ctttaagctt accacttctc atatcattta 108240

atttttctct acgagtttca gagtctattt ctccatgtaa gaagtagtgc tctacatcta 108300

aatcatttaa catttctgat attgtatctc catgttcgat gaagtttaca ataatcaatg 108360

tccctttatc ttgattatac cacttttctg ttagttttgc aataagttta tttctaaaat 108420

cattatttgt tataccttta tcataagctt ccctgtaatc atcaattcta tctatgtcat 108480

taggattagc tacaggtata atatttattg ttggtctagc agaatgacct tcatcaatta 108540

aaaacttatt agtagttcgt gcaataacat taccaaatag agcctgcaat ctcatccata 108600

gtaattcatc ttttttatct atagaacccg ttaatgcaat cctatacaaa gctttttcac 108660

atgtcattag attattatac cacgaatctg atttagagtg atgtgcttca tcaactatca 108720

taacagtcac tgaatctaaa aagtctctca tatcttgata tttatcatat ttcttttcat 108780

tcttttctct aacaactttt tggaaatgtg cattatgttt ttttaagttt aacaagacct 108840

ctgcatcagt tttactattt tggtaaatta attctaaaac atttaataca ttttgttcta 108900

cttttgtttt aggtgttgta ttatcaagta atacttttaa taatttcttt tggtttgtac 108960

caccttcaaa tttaggtaat atctcttgtg caattttttt actaatattt tgtttaggtg 109020

taaccttaac cccttgtgta gggtctttaa ggtttgcatt caatgtaggt atcattacaa 109080

ctgtaacctg cttaacatca aacttacctg caccaacttt acctatagga atatttaaac 109140

gttctcttaa tctatctgca gattgatgga atatctctgt agagcctgtg aaaaatgcta 109200

ctctttctcc tttttctaac tgaggtaata gttggtctat aataccacta gcaacttccg 109260

ttttacctcc gttagtggct aagtgagcaa taccattgta aaatgttaag ctattatata 109320

ctgcttcata ttggtaatct cttaaagtaa tttgaccaat attattatca agtaatgtta 109380

tttcatcatc aatatcttcc tcagctaaga aactttcatc acgctcatca attgtttcaa 109440

actggaagtt atatcttgat tgtaactccc ctaataataa ctcaatctta aataaaagtc 109500

cagtgggaaa tttatcttct tcataaacat aaaagtctac atacccatcc caactacctc 109560

ttttgtaagc agggctatac tggaatccct cctgccttgc tcctatagtc gtatgaactc 109620

tctttaaaaa tatatctttt aagtatttat catcctcctc aaattctaca taggtatata 109680

agttcttaat tcttaatctc attttaatct ccttatatcc ttacacgagt tatagtatat 109740

cacataatcc ttctcaagtc aagaaaaaaa gactaggata agtttcctag tctatggcat 109800

attaatacct ataattcttg taacatattg agttttacta ttttgcactg cgctatctac 109860

aagagtccat gttgaacctt taaaggatac acctactcta taaaattctg taattggtgg 109920

ttttgtatcg gttctatctc ctgatacaaa tgtacctgag caaacgtagg taccactacc 109980

gtctagataa aatttatgac aaattgtacc tgtatcatca ttacctttag ttttctctga 110040

tgtcttatgt ttagtataaa cttctacata agaataatta ctaatatcat ctgctaaatt 110100

cttagtagta cctgtggata agaatgattg tgctcccgtc cataattctt tatgtgacgg 110160

tgatgtttta ggaaaagtcc aactactacc tactactgag cctgttacat ttctaccatc 110220

agtacctact gcattataaa caccatgtaa actagatgta ttaggtgtat ctgacatgaa 110280

taatcctcta cagctattag acataggaga acctgtcaca ccaccttgaa cataaaaggt 110340

aaaggttgtt tgccctgttc tatctatatg gtctataaca gcctgttgaa aagtcttatt 110400

atcactagtg tacacaagta atgggaaaga tgcactacca tcatccctga tactacttaa 110460

taaatatttt tgcatatctt gaggttctaa ttcttttttc cactctgtgt atacacctgt 110520

aggtttttta atactagtgt attctttacc tgtttcagaa tcagttaact tagctaatct 110580

attatcatta gcatctatat tcaaatctaa aaatcccgaa cttacacctt ttggcattcc 110640

tcctgtcatt cccataacaa agtaaatacc tggtgaagat atatccagga tattagttcc 110700

tgaaataaca gcatttttat tactaggtaa tccagttata aactgccatt ctgaactaga 110760

gtttcccgaa acaaacctat ataatgtctt aatttgattg ttattagtag acaatgtata 110820

tatatgctgt actactctag tagatgaatc taaaggtagt acatgaagta aaacatctat 110880

tgattccata tcaggtttat ctaataatgt agctaatgtt cttgcattta tataataata 110940

acctggttct tttatatccc taattacagt tatattccca taagatttag ataaaccctt 111000

atcatctgtt aatttaactt tttgtgattt attaaaatta taaactaaat catccgtaat 111060

gtcaaatata tttctttctt ttgttaattc ttctccaatg ttattgacct gaatagtcag 111120

gtctctaata acattgtttt ccgttattgt agtaaaattt aatgccattt attattaccc 111180

cttttgacct aagagttgtt gaagcatttt catcatagct tcttgttgct gtctcataag 111240

ttctagctct tcttttaaac tatcatattg ttctttaggt acagtatttt gttgagattc 111300

ctcatcacta ttattaattt gaggaggtac atagttttta ttctctataa cctcttccgg 111360

ttttttatag tataggtatc tatcaagagc aaagttatca aagaagtaag caggtaaaat 111420

ttctctatta accttaacct gaccaaagtc ttcatcataa gctcctgccg ttgtaaatcc 111480

tgtaatatat ttaccatctc taaggtgtaa tactatttcg tctctaccaa tatcaaattg 111540

tctagccatt ttatctaacc tcctaaagtg tgttaattcc tgctattcta tatatagtta 111600

ttattccttg tgcattaaaa tctgttacag atgttgtatt agtagaccca tttaaagtaa 111660

cctttttaac catagttggt gttgctgaag tatttgtagg gaaagttaaa tatccttcaa 111720

agaagtctac tcctgaacct gtagagttgt tagataaatt aaaatctctt atatataaat 111780

acggagtcgt aggtattctt aaatctaaaa acattgtttt ataatgtccc gctgattgag 111840

tcagatatac aacttctaat aagtcatagt tagtccatga atcatttaaa ggaatagtat 111900

cttttgaacc gtaatccaaa gtgccttccc aaagtattgt agatgttctt tgctgtttta 111960

aaggcttcca accttggttg taaccaccat aatataatgt atatggtata ccttctgtat 112020

ctgtaaataa taacttaaca aacttattag ctctataagg gtcaccttta tcataatcca 112080

cttgtatagt tcctctaata gagcgtcctt taggtgtccc ttcaatagaa ccttgacaat 112140

agaaagtatg atagccttga ggtacagtca tagctaaatc ttttaagttt ttctcattaa 112200

cttctgactt ccaccattgg tacttgaagt aatcattata ctcaatgtct acaggggaat 112260

gaccctttaa ttcatcttta ctaataactt cctgccaatt agaccaaatc tcatcatatt 112320

tacgaacatc gtttacatgt gtaataaacg gagttacttt agatgatttc tctaatttag 112380

gtaaagcata atatactact gcaccatttc ctttactaga agaacaattc attcgtaaaa 112440

gtagtgacac ataattaact ttaacacctg acgcaccaat cgtattttca ggtattgtaa 112500

atgtaaatga atactttttc cactcagtag ttatttcact tgctgttatt tctcttcgac 112560

cacctgtata aggattatct gttctatcta cagtatcata tcctgccagt tcaaaataaa 112620

ctaagttatc tttaagtaaa gcagggtcat taattttagc atatactgat acagttatag 112680

tatcccctac agaaagctca ttacccattt taaatctatt agtcattgct gtagggtatg 112740

tgctattacc atcatcaatc tgtatagtgt tttctcctct aaatctaact cctgaacgac 112800

cactattagc tgtatatccc caaaatggag gtgtagccca attatccgtt ttaatgttag 112860

aagagcttgt ttcaggataa acaaataaac tatctctaag taagttaggt ttttgctctt 112920

gtactggtag ttttcttatc attttaaaag ttgaagtaga gttaactgga ttaaattcta 112980

ctaaagaaga aacaccattc ttactttctt tataattaac ccatccagtg tttaagttct 113040

ctttaggagg attaactagt gttgcatatt gtgttgtaga ttcagtaagt tctttgacgt 113100

taatagtatt accctcatta tataaactac cttcatctaa tttaaaacta atccaatcat 113160

atactgttcc attagcataa gtcttaatat acattttatt actgctataa ggtgaataat 113220

atattttgta ataatttaca tttcttttat ctaatcttac aaatcctgct gttgcttctc 113280

ctgcaggagc atttcttgaa ttagttacat aatagttacc gctatcaggt atagatgata 113340

aaatattatt aaaatcaggc tgtgatatct gttttacatc actaaggtta acctctgttt 113400

ttaaaaagtt accaatatca tttaccttcg ggtacatatc ttttaacttt tgtgtttcag 113460

taagaggcgt gtaattaaat gccatctatt tttctcctct ctctttcttt aattcattaa 113520

gaagtctatc aacttcatta attttttcct tatatgttct ttctaattcc ttaattgatt 113580

tttcttcaac tgtaggtata aatagcttag cttgagaata agggtctcta acttctctag 113640

gcatataaat ctctccttat taaataaaga ggggtcaatc aacccctcta gtttattcat 113700

ccctagtagt aaccataagt ctacgaacac gaggacgtaa aaagctattt tcagttgata 113760

aatctaatct aacttgtagt ttagtatttg ttcctgatga ttttactttc tcgtcaatga 113820

catagcgtgt aaactcatta ttggctctag tagttgtagg ggatttagta aatgttttcc 113880

aagtttttcc atcgtctgca gaatacttag gaacaacttt agtaccttta ggtaagaaag 113940

cttcataact aaatcttatt gtattgtaag gagcctctgt catatcaata gctctaccaa 114000

catatgaacc tgttaattct gttaagaatg tagtgaatgt taaatcacta gaactcatta 114060

atggtgagat atatctattt gattcaaaag ttgctcttag tttaacttgt cttgctagac 114120

ctaaaacatc taagtcttga tagttaccga taggctccca tttcaactgg tcgaatgttg 114180

tagaagatgc catatcatct agaattaatt tcatttccca tgtacatcct gttctctcag 114240

gagttaagta cgtagacata agaacgattc tatctgcaga tacatcttta attggttcga 114300

attcaattgt tgctgtttca ttaaatttag aagtatagat accaaattta aggtcagagt 114360

tttgatgagg agtccatgtt gatgcgtttg atgaactgaa taacacacct tgaagatatg 114420

ggttacctga gataacctca ttaggtttat caatcttagg cttagttcta gtacctaccc 114480

acattgtata atcactgttc tcagtaataa taacaatagc gtattcttta ccgccttcag 114540

ccatcatagg gtcatcaaag tatactctag tttcagcact agcattatta gatactttaa 114600

tgtcatctgc attcataact gtctctgcat agattgtttt attaggataa ccttggtcac 114660

ccatacctct aatttggata acaacgttag attgtttatc acctttagaa gcaaagtata 114720

accctagtga tgatatagtt ctgttctcat catactggaa tgattgtgct aatgggtcta 114780

ctaagtttac tgttacacga gttctaataa taatatcttg aacgattttt ttacgtcctt 114840

gagctgtgta agttgtagca cttgtagagt tagcattctt gagtgtaact tctcggttac 114900

cacaacgaat acctgcagga atagtaaatt tacctttagc tgttcctttt gcatctgtca 114960

taatagttcc atcttcagaa ccttttctgt aaccagttgc aggggtaata gggcatctta 115020

caccatcaaa taataaatat aagttattat cattagggtt tagaccttta acctcgaagg 115080

atacatctct aattctaatg aattcaatca tttcttctag agtacgctgt cctcctgatt 115140

ctaataaagt accagtacgt ccatgctctc tatcgtaagc gtaagtttca cctttccact 115200

tttgtcctgc atctaattgt aaattagaat acaagtaatg ttcagtctca ccatagtaac 115260

tttcgttgtg tctccaaaat cgtttcatag ttactttctt agttttttgt tcagttattg 115320

taacattttc agtatcaatc cagttatcct cactaggtgt taacttaagt acaccttgtt 115380

tattagggat attataaggg ttaacattta atgtttctga tgcttgacct tgatagattg 115440

tacgttcctc agtaaatggt gctgaaatta atctacccca aatatgagct gttgtatctc 115500

cagtaataat cttaggttgg ttaactgctt ctgtatacgc tagagtagct tctgcgtctt 115560

caaaactaaa tacaattccg aagtcaggat gtgtaatatc tgctttgtca agactaatga 115620

aaccttcact aaatactgaa cgtagcgtta aagggttctg tccttccata gcaccatcat 115680

ctagagcatt tactgcttgg ttatactcta agttatctac tcttgtttta actttctgta 115740

agtcttccat agacaatcta gtgattgcaa acgaaataca tactgcttca tctgaatcag 115800

gtaatactgt aaccgtacct aattgtaaat tctcagggtc tgtatttaac ggtggtgtca 115860

ctaatctcat aatattaggt tcaccaggta gtattgcaat atcaccatac ttattaataa 115920

acactgaatc tttacgagcc aggtagtaag tatagtctac taatactact gtttggtcaa 115980

taggtttagc tccatttgaa ggtgtaaagt taatgtacca tttcttactt aacccttcac 116040

cctgtgttgt tacttcataa tctttaccgg cttccatacg tttattatat ttataagaaa 116100

catagtatga ggtaccgcct gaaggttctt gaccttgagg tgaccagtca atagtttgac 116160

catctgttaa tctgaagtct tctccttgtt tatactcttt tgtagtaact ccaggacttg 116220

tttcagtcca aacttttaca atttcaaatg ctgtgttatt tgaaagaaag tcttgaccat 116280

caccttgagc tcctcttgta actcgttctt tttcaataag tacttgacct gtaacacgtc 116340

taatttcttt tacagggcta ttagctaaac taatagaatt attagactta ttaaatatag 116400

tactttcgtt ttctgctgtt cctaggtcat aagatttagg tacactaatt cttgttgata 116460

ctggtttatc tactttaaaa ccttttacgt atgctttacc tgcatctaca actacagaaa 116520

cgtggtcatc atcctcagcg ttaccttctg agaataactc aaaaccattt actttatatg 116580

aacctgactc atcgtaagta cgttcagcta atactttatt gattttatcc atttcagcat 116640

tagttgattg gatatataaa tctccatcca tgaaagtata aatagttgct gaagtagggt 116700

cattaactgt taatgacatc ttttcttcta atctatctgc acctttggag aagtaacttg 116760

gtaccccact agtttggtct aataggctag catcttcatc aggtgtaaca atacgttctg 116820

ttaacttaat accgatagtt tctttaccta caccagttat tttaactgaa tcatcattat 116880

cgtaatatct tattttaccg ttgatatata cataaccagg gtttactgtc aatacattat 116940

cttcagacaa tgtgaatcca agtcctgatt gcttatctcc gtctttaaaa atagcgtctc 117000

ctagattttt taaataatat tggtctatag actgcatttc atttaattct gcctgttgta 117060

gaggtctatc aggattaaat aatacttttg ttctatcttt agacgggtca aatctatcta 117120

aataaggtga acctttaaaa ttaattgcca tttagaatta tctccctttc tttaaacttc 117180

tacaataaat ctttctttag cagttgtttg ctcacttcta ttttggaact gtttattgtc 117240

aaagaataat agtgttccaa ttgattctac ttcactcggt actaagttaa atttactaat 117300

accacttttt gctactaagt ccataacaaa tcctacttgt ctatatgttc caagaggtag 117360

ttcgtcacca acaatactac tttctaaata aacccattta gctccttcag ccttagcatt 117420

ttcaggtgtt acttctaccc acgatttatt accataagaa attaaattct tattatcatc 117480

ttcaggtgat ttagaaggtc taactaaagt aactttagta gcttttttgt atccaataac 117540

ctcctgtaat actgttgcat tttcatcagg ttgcggtggg tttgtttcat tagaccacgg 117600

tgtgctcttt ccaattgtta agtaaactga atcagcttta ctaactagat attttgctaa 117660

ctcaacatga gaattatacg ttgcaattgc catatttttt aattcctttc atctgttgtt 117720

ttctttatta atataacatg gtatacccac taaatattac tttataagta taccatatta 117780

ttaactttct gtcaacttat ttattaaact tttattaact ctagactgta aattgtaact 117840

acaccactat catatggttg agcataaact ctataatcat tattaagtct ttgttttcct 117900

tctgcataat tatcatctct agaagtataa gtaatatcta ctataccatc tttaatattt 117960

tcaggataaa caaatacttc attaaatgac ccattagaat tacctatagc taatctttct 118020

agtccttctt taaaatctcc atgtattctt aggttgtatt gttttctttg ttctataggt 118080

tctttcaaat tatatttagc aaatccattc tgtgtaatcg tttgtgtttg ataagagtta 118140

ctcaatatat tattattagg taattgctca gagattgagt caattacttt aattctatta 118200

tcaacattaa atgattctga tgttagtaat tcttctttta gctcacttat caatctacta 118260

gaacctaaag ttaatggttc agtgtaatta gtcaaagtat aagctaacat atctaaatca 118320

gtataaacac caacgttaaa taaagtagcc ttaataattt cacctgtagc tccatcaaat 118380

actccacctt cagtgtcttt gaccattctc atagaaccta aatcaatatc tgatagttta 118440

tctatgtctt gccatacagc attaaaataa tcattaggca tattagataa agaaccttct 118500

tgattattaa gttcacctaa tttaacattc caaatagaac gtaatgatac ttttgacatt 118560

ggatttatgt tagagtaatc tactgtctgt aatccatata aatctattaa attattagtt 118620

tctgttgttt ctcctgtact aatattacct atagttttta attctaccca actatcacca 118680

tagtaagaat agtataaagt tacattcttt agattaatat ctgaattgat atttaaagtc 118740

tgtatattag ttttgatatt attaaattga attccttttt ctattacatt aggtttatct 118800

tcaggggcag gtgagtaagc taatttagag tcaccttcag ataattttag ttcagtaatt 118860

ttaactgtat taaagaacac tggaggggtt gatgctttcc catcagatgt atatcttcca 118920

gtataaatta ataacctgtg gtcggtaata gtaggtgctg taaaagtctg agttatagat 118980

actttgttac ctacggtgcg ctccatagat ttaacaccat taacaacaat tcctttggta 119040

ttactgtata aatatatacc atgattatca gaataagatt taagagtcag gtcattattt 119100

gttatttcca tatcgaaaga taaagtgtat actttacctg gttttagtac tttagacaaa 119160

taattagttg aatatatctc tttagcccat cctgatacgg taaactcttc cgtagatgtt 119220

atttctctag ctatattatt tacaccttta taagactgta atagattcct gttttctatt 119280

gtactataca catctaactc agtagattct ataggattat tatctgtatc tttagctact 119340

atgtttactg cgctaggtct tgtattctca taactaccaa ctaattttaa gtatcctata 119400

ggttggaaac ctgttttagt tataatgtct ccattcaata atgatgctat tttaaacgcc 119460

tctacataag tctctctaga tatatctaag taattttcta ctaatgcttt ataaggtttt 119520

attgtgtaaa ttcctcgttc atagtaataa aacattaaat ctagatagtt aactttaata 119580

tctactgagt ctcttttacc tgcatttatc tctaatctag taaacatatt taattcacta 119640

tttaaataat cttttatata acctatattg ctaccaatat ttttttcata gaaagataag 119700

ttactaattg atactgtaag ccatctatta atagaaaagt cataaacttg taatttaatt 119760

tctatacttt cgtcaggagg tataacagca ctgagataat aatcaatact taattctttt 119820

atataatcag atacaattct tctagcctca tcagattgta aatctacttg aggtctatac 119880

ttactcatat aatctctaaa attaaagtta ttatagagat aagatacacc ggaagtagtt 119940

tgcatattaa gttctactgt attattattc ttggtactag tatacatata ataatcagta 120000

ggtacttcct gacctctacc ttctgtacta gcacttattt ggtttacaga agatgtcata 120060

cctggattat aaacatatga tgttacatat ccatagttaa catattgtct acctacacta 120120

gaggaacctg ttaaaacatc taaactatta attaaactat gatttgtttt aaaaatgtct 120180

gatgaactat tatcagtatc tttactttga ttcatattaa tatgaccata aaatgtatca 120240

tcataacctg taaacctatc aaactcttga tatgtttcaa ttttaggtag tccatctaac 120300

cacttaataa ttgctccacc tctgatagta gaagctccat cataagtaac atataatgtt 120360

acacctgcag gtttaaattc gttaattaca tctataatct ctatagggaa atagtcacct 120420

atagaaacat ttataacagc aaatctataa tagtatccca ttaaatggtc ttcaccattt 120480

aagtatgatt tgttagtata gaaaatattg gtaaagggtt catacacgct tacatcaata 120540

tcatccttac ctaaataatc tcttatagca tctattatag cattgttagt tcctcttttc 120600

aagagtaagt attttataat tcttgctcta taaacatcat ctttttcatc ggtctttcta 120660

taaaccccaa accaatctcc aaatttgtct aaatattcac tcgttgctgt ctttagagaa 120720

gattgtaact tgctttctat agcatctttc tctacttgat tcatctcttc attgagtgca 120780

tctatcagag caaagttagg gtcttgatta tcttttttat ttctatctct tcttaataat 120840

ggatgaagat tctttaaaaa attagccacg acttaagttt cacctctctt tactttaatt 120900

ctacttttat ttctcctgct ctaataattc cttgtggtgg tactataatg ttctcatcta 120960

agttctcaaa cgatacatca tatattaata catcatcaat attcattata gcttgaataa 121020

ggtctgtaat tatcaggtca tcagaagttt ttaaattatt taagtagctt ctaataacac 121080

cttcaatatg tttttgcaat gtatcaccaa ttctagattt attagatata gttactgtag 121140

cagaaacatt aacttcttct ttttctacac ctgtaacatc taacattata ccactcggtc 121200

tatagtcttg taaagcatca attatatctt cttttaaagt atctgataaa ttaccatttc 121260

tatcatgtgc aaatactgta atatgtcctg tttcttcata aacataaaca ccttctacat 121320

caggtatctg caatgctcca tatcttaccg atttattagt tgctctacct ctagattcta 121380

caaaagagtg aaatctacgt ttaaagtctt cttggctttc ttctttagtt cctgtattaa 121440

aagaatactc attattaaca cttctaatca aactagaacc tgatgctata gtattaattg 121500

ttccttcagg aacattacct gcaactcctg tttctttaca ataaacctca acaacaatct 121560

cagtagagtc aggctctgca taataatcaa ctaatgtttc aaattgctga gggtattctt 121620

gtcgtgttga agtaaaagtt gttcctgcag gtatatacat tctcatatct aaaggttgat 121680

aaaattgaat gataacatca ccataagctc ttttagattg tctcttttgg aaatcaaaag 121740

cttcaataat accttcttgt ataccccaat caatattttc ttttgtcaag atataaaatt 121800

gctctatctc taatgataca gcttctaata atgacctaga ggctgaacca ggggtaaaat 121860

ctgttatctt acttgtacct gccattgtct tatctattaa ttttgataat atgttagtta 121920

attttctagt tttcataatt taatcctttc ctattcaaat aaagcaaaaa ttccttcctc 121980

atcttgtcct aaaacaaaat ttattgattc ttcaacagat tttatttcca ctgaaaattg 122040

acctgaataa acattacctt gtattttcca atcaattaga ttagcagatt ttactctatt 122100

atctgatgtt aatgttctca atacttccat ttctattaat gtagcttgtt caggtatatt 122160

aagaccaaat aaattatgca aatctgagcc atagttaggg tgtaacatta aagaacctct 122220

aggagttaat aaacgtgctt gtaactgctg tttcatatta tctatgcctt ttacagtatc 122280

taaatcccca ttaccatttg tactaaatgc cagtatgtta tcactagtac cgtgttcatt 122340

aaaatacttt tcatctgcag taatatttaa atctcttcct aaagctaact caactaatac 122400

atctttatct ctagaattaa tttcctttgc tgatacatct gttaagtctg attcaatagg 122460

tataatcaat gtatcacctg tagaagctag cctttcaggg tctttcattt tttcttcatc 122520

agtttctact aggtaaggat actttaagtt attatgttct actaggtcta tccaataact 122580

aacatcacca tagtatctct gtgctattgc ttgcatagtt tcttcatgtt gaacaacatg 122640

cttcttaaat ctcattatac gttagcctcc tttttaatta gaggtaattg taactctata 122700

gcactaaaac ataaatctag gtctcgtaaa tctaagacaa cactattgta atcatcatag 122760

ttacctaaat agtctgctac ataatttaaa ttttctcgtg ttattctaac atcttcttca 122820

gttagatatt ttaaagaact tggattttct acaaagcaat taacaatagc atacgcttca 122880

agtacaatag attgtagaat catatacatt ctaggggctt gtacttttag taatgtatcg 122940

ttagctttat gaataattgt attgtcatta aattctcttt ctaatacagg tatatcatct 123000

gtctttatat ttgtcaatac tttttttgct aattcactta attgtaattg cggtgtataa 123060

aatttagata caaatgatac ctcttcatca agtaaattca tatgagaaga agttaaatct 123120

gtatctaggg cgtttaaaaa cctaagtaac ccttgaggtt gtggtataaa attattcatt 123180

agctagatac acctccatca ccataaccga ttattaaagc catattgtca atattacctt 123240

ttaatccatt agtagattgt ctaggattat atatagaact actagggaaa ctttgcttta 123300

acctattacc ctcgtttgta ttatctcctg tagatttagg tattgtattt tctcttctag 123360

atatttcttg gttatttcta tctctcgttt tacgagcatt tttatctagt tcttttatgc 123420

cttcatctac tctttgagaa gcgttaggtt taacattacc aaactcttct gttgttacag 123480

cacttctatc tgcttctgtt aatgaaccga tgactactaa tgttatttca tatctaaaaa 123540

gtaacggttc atccttagac ctagttacct ttaacccttg aggggctaaa tgaactttat 123600

agtagctatc atctgtaaag ttaaaaaatt gtaaataaga gccacttacg ttaccactac 123660

caccttgcat agcatattca ctaactctac tctgtaactc ttccatcttc tgcttacctg 123720

tttttaatcc atctgcttct ctaacaggtc taaaaccagt tgtacctgtg aagttaataa 123780

cttctatgtc tttaccataa tcttctatta caatatctga ttttgtttta attgctgtcg 123840

tacgttgtgg tgaatctatt gtgtaattct caggattaac tttaaaccta tacatatcat 123900

aaccaccgcc ttctttaggg aagcgtaaag ctattctatg aagattactt attccatctg 123960

attgtggcat tttatcctct ccttttcagt atgtatataa atctcatact actaatatat 124020

cacgaactag tttaaattac aactaaaaaa agacctaaat taataggtct ttaatattaa 124080

atctttttat attcatctag gtaatttgta aagaatccat ctacacccat ttgtattaaa 124140

ctttgcattt cttcttttgt atttaccgtc catgcatgta ctttaagccc tttgctatgc 124200

gctaaatcta ctaactcttt agttatcgtt gtatatttag gagcaatagc ataagaacca 124260

taacttaaac aatcatccat ttcactttca gagaatgtac ttgttaaata agctaaaggt 124320

atattagaaa attggttatg aatattaatt aatgattccc tagcaaatga ttgaataatt 124380

acttggaatc tttctgaacc aattcctatt aatccttttg attttaactg agttaataat 124440

tctttatcca tattagcatc aaaaggacgt ttagtttcta tatagtattt aacttttgtt 124500

ccaaaatgat ttaatacatc atcaagagat ggtataggct caccattaag agatgtaaag 124560

ttagtttgta tataagataa agtcatatcc cctaccttac ctgttcctgt tgttgttcta 124620

tctatagtag aatcatgcat acacaataac ttattatctt ttgttaattg taaatccaat 124680

tctatataat ctgcaccttt atctaaagcc atttgatacc cttttattgt atgttcatct 124740

gtatatccag ttgctcccct atgacctata agcataaagt ctttctccat ggtttcagtt 124800

tctaattcta tagcttcaat actaaaccat gtataagggt ctgctctgaa ttgtctgtca 124860

ccttctgaat cttgaaaagc ctcaaactca aagtagtctc cttctttaac aggaataact 124920

ccactagtac cattctgagg tgcggtagat atagctacat cccttgtata aggtaaccct 124980

aaactataag taccattttt caatattctc aacatacgtt gtcctgtagc gttagaatcc 125040

cataggacat tacctgcaac tcttactttt gttattccct taggtactac taatctagta 125100

gggttattag aatcccaaaa ctcagcatta ttataagtaa ctttactcca aggtattggc 125160

atatattttc tcattggtac agtaacattt tcctctaact gtatcatagc acctatatgc 125220

tctcctctat ctgaaattat tttatctgca ttgttattat tattctctag ttctgttaac 125280

ctatttttga tatttggtat ctcagttttc tctaagttat taactttagt ttttagatta 125340

ttaacatctg ttttcatact agggaaattc tgatttactt cattaataac agtagaagca 125400

tcttcataaa agtctttaaa agtctcatct ctaaattttt ggaattgagt tataataccc 125460

tcagttctac cactaacctc tgatatctga gttgtaagtc tattaacatc tgaagttgct 125520

tttttagacg cttctataga ctcttttgtg gattctatta gttcttctaa attatcctta 125580

cctacaccct caagaagata gtttatatca tctagttctt tttgtatttc atttaaattc 125640

ttcatagcct ctgctatact ctcatctaaa ttttctagca taggcttatc gtctatataa 125700

attccttcat cagtaaattc aaatttatgc ttatcatttt taatataaaa tttattatct 125760

tcactaatac catattcaat ccaaacttga gcctcatcca acattaaatc gtcatcctgt 125820

ttaataaccc tataagaacc atcacttgac atttcttgtg tagttctttt ctgtgtttct 125880

gtatttaaaa ctgaagctct gatatctcct ctttcagata taaataaagt agttatgtga 125940

ttatccggcg tgccatcatc ataaaaaact ccttgatgtt tgaataacat attaggagcc 126000

ttttgtattc taggttcaat caatgtttta ttaccataat aggaagtaaa taaatcttga 126060

tactcagttc cagtataaaa atctgttgcc tgcggtttct cttcttcacc tgatgtcata 126120

gagaagaatg atttaccatt ataagttcta atacttgtcc cttctccatc atcatattta 126180

taatttaaag atggtaaaat ttcatatagt gaactactgt atttgtaaac actttctgta 126240

tcaaacttac ctccatctaa ggggttagta ttaatcattt tattttgttc attatcacca 126300

taaacactca agattatagg actatttata tcatcattta ggaaccctat taatactaca 126360

gaaccttcag taataagtgg ttttgtaccg aatacgcttc cctcaggtgt tctacctatg 126420

aaagatttag gataaggtac agctaagcta ccatcatcac ctatacggct tcctaaagtc 126480

aagttattaa ctttaacttc tactgattga tacttataat taattttact aactgtagct 126540

aaaactaacc cttttacatt taagtcatct gaatctactc tttttagact tctccccatg 126600

gaagattgga atcttactac cattaaatat acacctcttc atcgaatctc ataactcttc 126660

cgttaaattc attccagtaa tcatcaagtt tgtattttcc tataggtgtc tcatcttttg 126720

gaaatttacc tatcatagat ataaactctc catcacctac ataaatacct atattagtat 126780

cacttctacc aaagaataat atatctccaa ctataaattt actaacatct actttatact 126840

tatgaccttt accaaaaaca gtaaaaaatc taccttctgt aaagaaatag ttaatattca 126900

taggatatgc taattcttca tcttcaccta aatacaacca atataataaa ttataagtat 126960

ctatataaac atctttatct gttccaaaag gattatcttt aggggctcct ttgaacatat 127020

actttatatt tgataaggat ttaagattat catttaagtc taatgctagt atagtactta 127080

atggtacatc ttcatcaaat acatattctt cttccatata ctccccatac aacacatctt 127140

gtatctcatc aaagtaatct attaataact cctgatttct ttctactaag tgaactgtta 127200

aagtggataa agaatctgta ggtatagacc ctttatcaac taaataactt actttcctaa 127260

aacttaattc tgcttctata tcgtaaccat tattagaaaa tgattcatat tgtacatgtt 127320

gagggttaag actttgtatt actccaacat aaggtcgtat atacacaact ttttcataca 127380

tatcatctgt agttattttt ttgatttctt catctttttc tttaactctt tccgtagtta 127440

atacatcaaa attaaacaca ttatctataa atttatcaat aacatcttta gcttctttat 127500

ctgttgccat agactatcct cctttatttc ttatcgtaaa tgaattgagc ttctttacct 127560

atttgagagc tcatgtctat tgttctagta ccataaccat taggcgtagc ccagttatgt 127620

tcagaaatga atattttctt cccaccatct aaaacttttt ccacaaaggc aacgtgacca 127680

taagcattag aaccaccttt gtctcctcgt tgccaaataa cacaggcacc ttgttttggt 127740

gtcttaccta ctccatatcc tgcactttta gcaccaccaa tccagtcggc ggcatccccc 127800

cataaaggca caggaatgcc taattgacct cttctattat aagcatacca agtacattga 127860

tacttgtaat gtcggttacc gggctgaacg aaacttgggt catgtttagg aagtttacca 127920

ttatattttt taagtgaatc tagagaacct ccatcttgtg ctccaccact atcagagcca 127980

ccgtctttat ctccactact tttcttctct gctacacctt tttctttgag ttctttagaa 128040

gtatcttcac ccataagacc tcccatgaag tctgatgatt gattccataa acctgcaaac 128100

ctatgaggac tacccttacc atcttctagg atagcatctt ttaaacctct agttactcct 128160

acagttgtat aataaccttg tttataatca aatttatgtt ctacagactc aatatagaat 128220

tcccaagtat ctccccgttg tttatcctca ataaataacc ttttacctag gtcatactta 128280

gggtctccta aaacaataat atctcctgcg tagaagttag ggttcccatg ataccaatta 128340

aatagcattc tagagaacat aactaaagga ctatcacttg catctgaacc tgtgtctgta 128400

gctacgttac taacaccttc aatagcttta agatatttat caaaagcttc atcattcggt 128460

ggttctcctt tgtatttaat atattcatct aacaaagttg tagcgtgtgt tttgttaccg 128520

aaacgatatt tagttgtcaa ttctttaagt gcatctgtct tagctttttt ctttttctta 128580

gagttattaa aatcatcttg tgttttaaac ttctctttta gtatactctt taatttatcc 128640

tttgtcaact tcggtctgtt atctgatgtt aattcatcat ctactttatt acctgttatc 128700

ttctcgtatt ctttttctgt tacttttcct tctttgacaa atttctctat aattttttta 128760

gcttgagctt tttttaagtt tttatatttt gaagataatt tacttgtata cttatctata 128820

cctttagata tattatctct tccatagtta cttaaatctt tcataatttt agagtaagtt 128880

cctctttcag taccattgtc atcacccgaa gaatctgagt cttcagtagc tgaacctgat 128940

ttagtactaa gatagatatt ttctacttca aatttagtat acccatatct atcagtcaat 129000

tcagggtgaa attgtggttt agaaaataca tcaccattaa gttcttttaa catacctgca 129060

ggtgtagctg taaatataga atatgtctct acgtcacttt tacctacatc ttcttcaata 129120

aagtcttcag taggtacttt aatcatatct aaagctctcc actcagtagg attaaaaggg 129180

gtttttctta atacaagttg tgctttacca ggtgtttttt cagagttttt gaagaacaat 129240

tcattaaagg gtctagctgt taccatatcc atcaactgtt ttaatgagcc atcaaaatta 129300

gtaaaagcag atacttctgt caaattttca aattcatccc aactacttaa atcatcataa 129360

tcaagatagt tatcaattgt attatatgtt ttttcagtat agttatactt catatatggt 129420

atgaatctac gtataattcc tgtcattact tcatgggctg agctaccagt aaattttact 129480

tcattatctc cgtcaccatc aataagccaa cctacttcag gtaatacagc ttgaacttcc 129540

tgaataactc ctaatccaaa tttcataaaa ggttttacaa aagattgacc tgttatccta 129600

aattgagttt ggtcattacc ataactacct acttttgata cttgtgatac catacctact 129660

tggattaaac gttcttgacg accttcttta tcattagggt catcattagg tgtaataaat 129720

attctgatta tatcgttagc cataactaac ttatcccagt aagtatctcc tgccatattg 129780

atttggaata cagaactatc atcttccata gagtttttag tttggaaacc taaaagttta 129840

gctccgaact catcaccatt gtagtcacgt gtatcttcaa atcttaatgt aaatgtatta 129900

tcatctgtaa ctatctctat tcttacctta ggtcttctta atcttttcat atacacatac 129960

ttccttttat ttttatatgt ataagagtag tatactatga tactactctt gtgtcaatac 130020

aaaattacta gtaacttctc ttatgttcat ttgcaaaaat atccatgttg ctgtctagaa 130080

cttctctaat tcttcctccg ataatgtctc cagtttcttc aggattattt ccaccttgaa 130140

cgttaacatt aactgttatt ctattagttg tagaagcatt agaagcatca ttagagcctt 130200

tatagttaga accagtagtt aatacagggt tactcatagc ttcttggtaa gtagaggata 130260

gagctcctcc acctcctccg ccaccggagc ctccgtattt cttcttgaat tctttagcat 130320

tgttaacacg agtacccatc atagcctcgt ttgctcccat acgttcaaat ccggtagcaa 130380

atgcttttgt attctgttct aagcttccgc ctttactcca tccggcattt ttaagattat 130440

tactttcata atcagactgc atctctttcc ataggtaatc taattgaaca tccatgtcac 130500

tagatttttt acctttagat ttagcaaagt tttctaatcc tgtttttcta gcccctaacc 130560

actgagcaat accaaatgca ccactagagg cattcttagc attagggtca agattagatt 130620

cttgttgtaa gttccccata acagctccta cctgattgtc agatagtcct tttcctttaa 130680

ggaacttata cattttctct gcgccttctc cacctacatc agatgcagag cctccgctat 130740

cagaaccact atcagaagtt cctccatcta tattaatacc tttagcactc tcaataattt 130800

tctgagccct atcaagtaaa tcactataga ttttaagatt cttagtttca gattggttat 130860

tcttttctct aagttgttct gctgtcattt tttctttctt cttagtatct cctttaacac 130920

ctttaacgcc ttttttctta tctttgctat cttcttcaga tgcttcagcg tcattatcaa 130980

agagacctgt tataccgctc catgctttag atataggttt tcctaataaa gaatcttgga 131040

atttattctt accgtcttta tctccattag tccatcctag gttccatcct tctttagtgt 131100

tagccttctt agccatgtct ccgacttttt caccaacagc tcctccacca aaagaaccta 131160

tagctccgcc tattaaagct ccaggtattg cacctacacc accaaataaa gctccaatag 131220

aagctcctgc tgatgcacct gccattcctc cgcccataga gcctatacct ccgccgatag 131280

ctttactatc accggaagct aaagcaggta tcaatgataa ggcagatgta gcaacaccta 131340

aaactccggc tccttttcca agtccacctg caagttttcc tagttttcct agcccgctac 131400

cgcctgcttt tggtgattgt gaaaggatac catttttacc accatcacca aacttatcag 131460

caaatttacc atagagacct ttagcttttc caccaaaacc tttagcttta tctcctagat 131520

tttgtcttct ttcttttcca aatatactat cattataaat atctttaaga ccgctaccag 131580

tacctttagc tatatcttta gatttatcca tggcttttct accattgtct ctgttaaata 131640

cttttctagt acctgaccag gcttctttag atgtttgttt aaatccttta cctttagatt 131700

ctccaccaaa catacctttc catcctgctc caccgactag gaatcctcct aatcctttaa 131760

cactatcaag tattttacct ccagtagaac gagtaccgcc acctttagga gagcctccgc 131820

ctttaggtga acctcctcca gtaggcattc ctcctgccat agggttacct ccggagctac 131880

cacccttatt tctgccaaat ttatttctaa gtcctttagc tcctttacct attaaatgac 131940

ctgccccaaa ttgacttgca gatgcaatta gtgatgctgt aaatgctcca atagctccta 132000

ttgctaaata tataggagca ggtagccctg ctaaagcact atgagcatct cttaatggtt 132060

gagccatatc ataagtatct tctgccttat catcagtctt agatttattt tggtcatttt 132120

ttcctgattt agattcttta taatcagtgg cgttatcttc tccttctttt ttaccttctt 132180

tttccatttt cttagctttt ttagctaact cttctttaga taattttccg gattgagcat 132240

ctttaaatat ctcatcagat tgctccatag ttaagttagc acctatttct ttcatacttc 132300

tattgaatag gtatttttgt tctttttcac taccacccat ttgagtagcc atatcagcca 132360

tgtctgtcaa gttttcaggg ttagatatac cttcatccat acgtttttgt aaatcatatc 132420

caccttcaag accttggtac tgtgttcccc atcccattgc tatacgagca taagaactat 132480

tcataccatt tttaagtcct tggtctatac tattaatagc attggcacct tgttcacctt 132540

gtaatccttt acttcctgac tctgcaaaag tagattgcat agcagtaaga ttactcattt 132600

ggtctttagt taaagttctt ccttctcctg attgttcagc tatagaacct aatgctttaa 132660

gttgttcatc ttgacgacca accatacctg attgtttaat cccgcctagg aatgcatctt 132720

gcatctcctt catgttatcg gaatttactc caccagtatg cattatctga cccatagact 132780

cttgataagc ttcttggtct ttaataccta aagaacgtcc tccaatagca agttgtttag 132840

ttcctgccat tgtatcctca tcacttttat gtcctacaga tgcttcatat gaagtagcca 132900

ttttcaacat gtcagtacta ttataaccta atttgttatc aattgacaag tctccatatg 132960

catttcttac agaatctata tccatattat ctgagttttg acctagtgat gtaaccattg 133020

gtctattagt ttcacttaat gaagcacctt tcatgtataa acccgtagcc atagctgtag 133080

ttcccatcat agcatgagaa ccgatagatg gtaaacgctc ttgaaccatt ctaccaaatg 133140

ttcctcgttg ttggtctaca tctctagatt ggaaatcttt ctgaactgaa cctttatagt 133200

attgaacagt tttatcaagc tctgctccta atttctcata gttagatata agttcttctc 133260

tagctttcat ttcctgctta atagaagcta attcttgttc atactgagct ttatttattt 133320

gaccactagc tctacgttta gtcaggtctt gttgttggtt tctataatta ctatattgtt 133380

ctcttgcttg acctaagttg cgttgatttt gttttctttg actttcataa gaacctatct 133440

tacctagttc acttctaact cgtgcccctt ggtcatatgt taaacgtcct gttccttctg 133500

aacgctccat ataccctcta gtcatattac gattagctct taaacgttta gtatagtctt 133560

tagtagcact agaatcttta cttatcttat tatattcttc tgttacttga tttaaggttt 133620

tcatttcctt aattctattt tgtatactag cgtctgagcc tattttttgt gaagacattt 133680

tctcaagttt ttcttgagtt tcatctacaa ctttacccat cttagcatat gctttttcaa 133740

cttcagggtc aggtgcaata cctttagata tattttcttt ttgaaggtca tctaaggctt 133800

tctttaattc atttatattc tttgtagcag attgagtgga tttaatagat tcatccatat 133860

ctttagaaga tatgacagat ttaccttctt gtttatccat tgagttctgt acatttttaa 133920

cttctttagc aattactttt aatttcttta agaaattgtc taaaggggca tcaatatttt 133980

tagattctag ggaatccata taaagttcta ttgccttcag actattctct aaatccgaac 134040

tatcaccgga taagaccaat ctataatcgt cattcattgc catcattatt caccatccat 134100

tttaaaaaaa taaggatggt agtatcacct accacccttt acatatacca gtcatcatct 134160

aggtcttcta gtgctttatt aacgctctct tgtgttatag tagcatcatc ttcactagtt 134220

ttaccatttc ctacttcatc atctaattct tcttgttttc ttctaatgta ttccatcata 134280

gcaagatgtt gtgtagtgag tccttctgtt tcatcattta actctgcatc atttctctta 134340

gctctttctt ccttatctct atcagataat ttagcttcca tctgttgtgc taagtcatcg 134400

gcatctaaga aatcaggtac cgggtcaaag tcttcatgag attcattcca ccaactacta 134460

tcgtcatctt caaattgact atcgaatttt ccaccttttt gagcttgttt aagttgttta 134520

ttatgttcat aaacatctct attcatatta ccaataataa actctatttg ataatcgtct 134580

aaatccttaa atctttgctc agtaggcaaa acattaaact ctttcataat agcccataga 134640

tttctagata aaggttgttt aacaattgat tccataccac ccaatcgttc tatatcttta 134700

tctaatgttt ctatactatt agtaacgaaa ggagtttaag aatcctaacc aatcttcata 134760

cataacagtt aaaggatata agttatagat atcatcaggg tcttggaatt ccttaggaac 134820

ttcaatacct acttcttgta atgtagctaa catttggtat gctctaatta cttggtctgt 134880

ttgatacata tccataccac ctaagtaagc agaacgtaac gcaaatatgt taccttgttc 134940

tcgtgcatta ggtaatttaa ttttaacttt aaatcttaaa tctaattctt caaagttata 135000

ttctttttcc caagtatcat taacaccttt aataactttg ttaattactt gttctttttc 135060

ttgcttatct tgatatttta atttatctat ttcttccggt gtcattttac taacatcttt 135120

tttatcttct ttagttgttt cttgtacatc ttgttccttt ttaatttctt ctgccataat 135180

atgatatacc ttcctttttt aaatattaat aaagcctacg cttatccgat tcaatattaa 135240

tataacacgg actagtgtag gcttactact ttaaacatag tatatcatat tttgttttta 135300

aatcaaaatt actttacata ttcacctgta cgcttagtga tttcttgtaa actttgttca 135360

atcttttcac tgtttctaag taggtagata tagtattctt gtaatgaatc ataatactca 135420

taatcaatag ttaatatttc ttgaccttct agatattttc tcgtaatttt ccctgcttgt 135480

tcagttaatg ttttaaattt atctagaatc tgattgattg caattgcagt tgatttactt 135540

gttacatcta cctctttagg aaatttgtgt aattctactt tcatttctgt gtaaattgct 135600

cttaatttat cttgtgacat tttatatctt ctcctttaat ttgttatatc tgtattatag 135660

tttattattt ttattctgtc aatacttttt ttacatatat tttaatgagt agtaaatacc 135720

attaatgatt aaaaattcta atagttgaat atatgatatt gttaataaaa gattacttgt 135780

agctagagtt ttttctgtta cctttttttt actatttaat ttactaaaca caataattaa 135840

tatgtttgat attataaaat aactatttac aaatattaat cccattataa ttcctcctta 135900

tttaattgtc aagtttttat tataatattt taaatctaat gttttatgtg tatctgtaat 135960

accattatag tatgtagtta ttattgtacc atcttctaag ttaataacta agtactgata 136020

actacttact ccttctatag ttacaatagt agggctttta aaaattattc ttctttgttc 136080

tttaccatga aaatctgtaa tattatactc aataatatta aatggtgcat ttctatcctt 136140

aaaaataaat cttactagat tactaaaatt aattttattt ttaagtttag ttttacaatg 136200

acttgatagt tctaaatact tatgcgtatt aattgtatct tgtaatagat taacacactt 136260

cttcttttct tgtaaagaca tttgagtata atgtttcttt acttgtctgt ccttacctgt 136320

tgttcttctt tttgaccaaa atgtactcat gtaattcatc tccttttttg ttatactaat 136380

aatatacctt agttaattat agttgtcaag acctttatgc ttattttttc tagaatattt 136440

tgttttatct tttttaacct tagttacagg gttgatattc catgttgctc tcgtattact 136500

catttttcct attgtttttc tcttatttgt caagataatc tttcctctct ttttgtaaat 136560

cttttttaag ttctgttcta gactttcttt tcattatatc tttaaactta cctttagttg 136620

ttggaaaatc ataataatga aattgattat aaccttttat tttagcttgt ctattattca 136680

tactcattac tcctaacctg tttattataa tatcatataa attttataat gtcaataaaa 136740

aaagaccaac tttttagttg gtctagtata ttaagtacgt gctttgtcac ttgctgttaa 136800

gtaactaaac tcgatttctt ctgttacaat ttcatttgtc tgccaagttt cattgtagtt 136860

atttgcagag caaccatgat atgagataat aacttgtttc gttaagttat ctacaactaa 136920

aatatcaatg atatctttct taagaatttc ttcaccaagt gaagcatatc ctaaatctgc 136980

aaagttttct tttttcatac gtaaacgttc tactgtaatt gtaccttcat aacgtaagta 137040

tacgtgttct tgaggcatga tagaaccgat ttcgtataca ccagttgtac cgtattcacg 137100

ttgacctgat gctgattgtg ctcttcctac cggtttacct ttaatcataa gtaggacggt 137160

attaccagta tggacggttt gtttagcttc tgatgccatc tgtattcact ctcctaaagt 137220

attcaaatta tattaatagg agtacctaat ccaggtactc catcacctag attaggcttg 137280

taatgtctgt tgcttgtaaa ccaagctaac agagattttc ttgaagcttc tgattgggta 137340

aactgtcatt gaaattcttg cttcgttacc ttcaacaata acttgtacgt cttcagcagg 137400

gaagtcttga atttcattat cacgtttctt acgacccaag taagattgga taaagtcttt 137460

aataattgaa gcacttgtat tgatagtacg agtaccaata aattggtctt caagttgaac 137520

tttaagctca cttactaaga agtcattagc ttccccaaca gccatttcag ccttaactgg 137580

gtctgattta tcattgaatg tagttacgtc atcaacgatt ctgaagaatg tattagtacg 137640

gttacgaaca aactctatac taataatacc attttcattt aattcatcta agtctattga 137700

ttcatagatt tggtctaatg aacttacacg taatggtttg aatgtgattg attcaccgat 137760

ttctaaacca cttgcaagac cacctaaagc tacagctacc atataagcag gtacatggtt 137820

tttacgtcca tcatccataa caaaagtacc tgagttagct actaatgata cacgtggatt 137880

agataatgat gcttgtctac cgaacaattg ttctttagat tcattgaatc ctccaccaac 137940

aatagctctc attggttctc ctgcgtcaga acgttcttta acgaaagaag ctacctctgc 138000

atgaactgat tgtttagatg ataatggaac aatgtagtat ccaccttcat gtgcaaattt 138060

atctaattta tctgcccatg tagcaggtgg ttcaccgtta gtaccgcctt ttaacttagt 138120

taattcgaac ggctcaatag ttttaatagg tgaagtagca gttactgtag ctgattcttc 138180

tcctgcttca acctctacat tacttggtac ttctccttct gcattaagtt gctcgaaaga 138240

tacaataccg ttatatgctg tttgtttttc taagtcaccg aaaactgctt taacatatac 138300

agctttgtct ttaatatctg cattttcaat tttatctaat ttactagatt ctaagttctt 138360

atctccgaaa ggtgataatt tagcttcgaa atcaggtaat tgattaatgt ctgtaataat 138420

agcattagta tagtcataag ctccgccagt taaatcatat gacttaactt cttggtctcc 138480

aacttttaat actaaacgac ttgatttttg agtttcttca tcatgttcta cagaaaaagt 138540

tgcgttagct tcttctcctt tatacttgat tgtgaagata ttaccgatat tatcataaac 138600

ctcattgaaa cggtcatctt ggaatattac tcttaaacgt aatgaatcac ttaatgtgtt 138660

cttttccaat cctacttgaa tgttgttagc aacattaccg aagattttag atgtaatttt 138720

tagtccgcca acttctgctg ttgcaggttt agcatcttct atacgcatag ctaaaatacg 138780

tcctgctgta tagttagggt tagaacccca tgctaattca attgcatcaa gtaattctcc 138840

tgaacggaat aaacgtttag cttgtgcata gttacgtaat tcataaactg tatttggttc 138900

tccgccttca gcctgaccga ttaagcaaaa tactttttca cttgaacctg ctgagccacc 138960

gatacctgaa gtatctactt caatagatgc atgaggacgg gtaataggtc ttcttgggaa 139020

tggttctact gccatattta attctcctta tctgaatgtt attatatatc taatttccta 139080

cctaaatact tctctaatga tggtaagaat tcttgctcac taaacaaata atgactacct 139140

tccatataag ctttaaatcc tgctacttgt gaatccctca tgttaaaaag ggtttttgca 139200

gtatcaagga atgtatctat atgaacataa ccattaaatt taggagtgtt attttcaggt 139260

gtcttttttc tagccatcct acttccaatc ctttctttct ttaaaagtta gtttattaat 139320

atcttgtgta atagtataat ccaagtctag agaacttgtg tacttaataa ttgtcggtct 139380

accaaaaatt attgagtcac cgtcttctat tattggtgca atatcaccaa aggacaaatt 139440

ctgtaattgg aatgtttgtt gctcttctat actatctctc atagatatta aaatcatttt 139500

taatacagca tctaaacatc gtgcaacatc tacattaaat gatagaccta caactgttac 139560

ttgttcttca actgtgaatc cttttactaa acctttagag tcgtttttct tttcagtaaa 139620

gataatatta gcattataat tctcataatc ttcatttgtt tggtacttaa atgatacctt 139680

attaccttca acttgaagat tatcgtattt agcaaactct atatcttcta cttttattaa 139740

ttctcctata ggtttagaaa cagtaaaaac taacttatca tcttctctta ttgctgtaga 139800

agattcgact aaggtatctc ctgttgcctc aaagtaagat gactgaatac tccctaaaga 139860

gttttttgtc tcttgtcctt gacctaattg tatcaagtaa tgagcttcat agttattttt 139920

aaattttggg aagttaaatc ctaccgttac ttcatgttgt gcattcttac cgcagaaagc 139980

ttctttaaag ctctctctag tttgataatc aaagtctttt aaaacttcat ctataatata 140040

acaattctct agcacagtgt taagtctagg ctttatttct gataataaat atgaatcaac 140100

tgatgttatt gccatttaat actaaccctc ctcttaattt aaatttttca tcttccagtt 140160

cattagttgc ttaacagttt ttagagttgt tttagagaaa ttattctcat taactttatc 140220

tctattaagt atccaggaac tagcaggtga tttactagaa acagttctaa atataaagta 140280

agaagattgc tttcctcttt taactttagt catgttatga gtcataggtt ccggtttcat 140340

tgacgggtga cttacattct ttctacgtcc ttctagataa tcagttatct tagaaactga 140400

acctgtgcct ttatctactt ttaaagtcct catatcttga taagtactgt tgttcattct 140460

actagtttta atgcggattg gtacagttaa ataccatcct ccgtctttac tagttttctt 140520

tttagatgag tgagcaaatg cttttttaag gtctactact ccttcttttt caagcttttt 140580

ctctgttact tgtagataag taggcatacg tttaatacta gtattctcta cttgctgtgc 140640

tgttgcttgt agcttgtcaa gaacttctga acgcatgcca tcaatggttc tcttacctac 140700

ctgcttatag tttttatttt taaataaatt aggtctcttt ccgtgaactg gcattattta 140760

aatgcacctc cgaagaatcc acctaaatta ctaggattag atgcggaagc tttagggtca 140820

tctacttgaa tttctaggtc ttcttctata ccatcattaa ctttataagg gtcttgtagt 140880

acaataacat cttccctttt aagaagtaat ttttgaggta agttttcaaa ttttgattta 140940

ggttgattaa atttagtata ttgataacga ctttccttta gtatatctga tactacatat 141000

cttaaagtca 141010

Claims (6)

1. An acid-resistant and high-temperature-resistant staphylococcus aureus phage, which is characterized in that: the staphylococcus aureus bacteriophage is staphylococcus aureus bacteriophageStaphylococcus aureus phage) Sa88P1, the staphylococcus aureus phage @, aStaphylococcus aureus phage) The preservation number of Sa88P1 is CCTCC M2020767.

2. A composition comprising the acid-resistant and high temperature-resistant staphylococcus aureus phage of claim 1, wherein: the composition at least contains the staphylococcus aureus phageStaphylococcus aureus phage ）Sa88P1。

3. The acid-resistant and high temperature-resistant staphylococcus aureus phage composition according to claim 2, wherein: the composition also contains a chemical disinfectant.

4. A composition of acid-resistant and high temperature-resistant staphylococcus aureus phage according to claim 3, wherein: the chemical disinfectant is one of didecyl dimethyl ammonium chloride with a final concentration of 0.05% or neoJielin with a final concentration of 0.01%.

5. A kit comprising staphylococcus aureus phage, characterized in that: the kit contains the staphylococcus aureus phage according to claim 1 or the composition containing the staphylococcus aureus phage according to claims 2-4.

6. Use of an acid-resistant and high temperature-resistant staphylococcus aureus phage according to claim 1, wherein: the staphylococcus aureus phageStaphylococcus aureus phage) The Sa88P1 is used for preparing biological bactericides and disinfectants, and the biological bactericides and disinfectants are used for killing or inhibiting staphylococcus aureus.