CN109136195A - A kind of vibrio parahaemolyticus phage and its application - Google Patents

A kind of vibrio parahaemolyticus phage and its application Download PDF

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Publication number
CN109136195A
CN109136195A CN201810959650.7A CN201810959650A CN109136195A CN 109136195 A CN109136195 A CN 109136195A CN 201810959650 A CN201810959650 A CN 201810959650A CN 109136195 A CN109136195 A CN 109136195A
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bacteriophage
vibrio parahaemolytious
application
phage
sample
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刘小琴
胡丽媛
王贵平
钱雪桥
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GUANGDONG HAID ANIMAL HUSBANDRY VETERINARY RESEARCH INSTITUTE Co Ltd
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GUANGDONG HAID ANIMAL HUSBANDRY VETERINARY RESEARCH INSTITUTE Co Ltd
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Priority to CN201810959650.7A priority Critical patent/CN109136195A/en
Publication of CN109136195A publication Critical patent/CN109136195A/en
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00032—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent

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Abstract

The invention discloses a kind of bacteriophage, DNA sequence dna is sequence shown in SEQ ID No.1.Phage splitting spectrum in the present invention is wide, can crack a variety of vibrio parahaemolytious.Thermal stability is strong, can be with the higher temperature in tenable environment.After flesh of fish sample using the bacteriophage processing vibrio parahaemolytious pollution in the present invention; the number of bacteria in sample substantially reduces in a short time; illustrate that bacteriophage can kill the vibrio parahaemolytious in sample, it is protectant to play the role of food safety, is with a wide range of applications.

Description

A kind of vibrio parahaemolyticus phage and its application
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of vibrio parahaemolyticus phage and its application.
Background technique
In recent years, aquaculture industry of China is grown rapidly, and income is rich, but anti-bacterial drug is long-term a large amount of in aquaculture The problems such as using, bringing such as environmental pollution, drug resistance generation and food safety.And the R&D cycle of antibiotic is long, Research and develop it is at high cost, so urgently seek it is a kind of can effectively control Animal diseases environmentally friendly new technology, new product again, use Partially or completely to substitute anti-bacterial drug.Currently, the still microbial control that people's concern is most, including Antagonistic Fungi prevention and treatment The anti-therapy of method, Bdellovibrio and the anti-therapy of bacteriophage, there are no pollution to the environment for these methods, and will not destroy ecological environment, more next More it is valued by people.
Wherein, bacteriophage is the virus for infecting bacterium, is widespread in nature, it is considered to be abundance and more on the earth The highest biology of sample, in most of ecosystems being investigated, bacterium and bacteriophage are symbiosis, they Abundance ratio is 1:10, studies have shown that the bacteriophage quantity in environment is 1031.And have the characteristics that host specificity is strong, do not feel Mammalian cell is contaminated, specific a kind of or a kind of host strain can only be infected.After infecting host strain, only infects host strain and do not split Other bacterium are solved, micro-ecological environment will not be destroyed.Phage particle can be quickly generated several hundred a filial generations after infecting a bacterial cell Phage particle, each progeny particles again can bacterial infection cell, regenerate several hundred a filial generation phage particles.It so repeats only It needs 4 times, a phage particle can make tens bacterium infections and dead.
Bacteriophage is used to have document report (Rong Rong vibrio parahaemolyticus phage VPp1 as antibacterial agent in aquaculture Application and its prepare with scale and preservation [D] Chinese Marine University in oyster purification, 2014.), but according to its test As a result, the effect is unsatisfactory for bacteriophage VPp1 cracking vibrio parahaemolytious.In addition, since bacteriophage has host specificity, only A kind of or a kind of bacterium can be cracked, thus makes Phage therapy in practical applications and there is a problem of that host range is narrow, it cannot Effectively control multiple bacterial disease.Therefore the bacteriophage of screening high-efficiency broad spectrum is the key that research phage preparation.It has reported Isolated vibrio parahaemolyticus phage fragmentation pattern it is narrow, be merely able to crack one or several kinds of vibrio parahaemolytious.Therefore, There is provided that a kind of cracking vibrio parahaemolytious effect is preferable, and the bacteriophage with broad-spectrum sterilization effect is necessary.
Summary of the invention
The purpose of the present invention is to provide a kind of vibrio parahaemolyticus phage and its applications.
The technical solution used in the present invention is:
A kind of bacteriophage, DNA sequence dna are sequence shown in SEQ ID No.1.
Above-mentioned bacteriophage is preparing the application in anti-vibrio parahaemolytious preparation.
Application of the above-mentioned bacteriophage in preparation treatment vibrio parahaemolytious infection medicine.
Application of the above-mentioned bacteriophage in prevention and treatment vibrio parahaemolytious pollution.
Further, vibrio parahaemolytious pollution is the germ contamination during aquaculture, transport and preservation.
A kind of antibacterial agent, including above-mentioned bacteriophage.
A kind of food additives, including above-mentioned bacteriophage.
A kind of detergent or disinfectant, including above-mentioned bacteriophage.
The beneficial effects of the present invention are: the phage splitting spectrum in the present invention is wide, a variety of vibrio parahaemolytious can be cracked.Heat Stability is strong, can be with the higher temperature in tenable environment.Use the fish of the bacteriophage processing vibrio parahaemolytious pollution in the present invention After meat sample product, the number of bacteria in sample is substantially reduced in a short time, illustrates that bacteriophage can kill the secondary haemolysis in sample Vibrios, it is protectant to play the role of food safety, is with a wide range of applications.
Detailed description of the invention
Fig. 1~3 are fragmentation pattern experimental result；
Fig. 4 is bacteriophage thermal stability determination result；
Fig. 5 is bacteriophage to flesh of fish sample bactericidal effect measurement result.
Specific embodiment
Below in conjunction with embodiment, the present invention is further explained, it should be appreciated that following embodiment be merely to illustrate the present invention and It is not used in and limits the scope of the invention.
Embodiment 1
The determination of phage splitting spectrum
Different bacterium single colonies is activated to logarithmic growth phase, 200 μ L of bacterium solution is taken to drip to 2216E solid medium respectively In plate, then bacterium solution coating is uniformly extremely absorbed with sterile spreading rod, 10 μ L bacteriophage stostes is taken to drip to 2216E solid respectively Culture medium flat plate surface places 30min in super-clean bench and is placed on 8~12h of culture in 37 DEG C of incubators.Observing planar surface is It is no to have the appearance of bacteriophage plaque.Transparent plaque shows host to phage-sensitive, and no plaque shows host to bacteriophage not It is sensitive.Fragmentation pattern experimental result is as shown in Figures 1 to 3, and wherein Fig. 1 is lytic effect of the bacteriophage to VP-DYW29, Fig. 2 bacteriophage To the lytic effect of VP-ZH 3-4, Fig. 3 is lytic effect of the bacteriophage to ATCC17802, it is seen then that bacteriophage can be split simultaneously Solve the multi-strain bacterias such as vibrio parahaemolytious ATCC17802, VP-ZH 3-4, VP-DYW29.
Embodiment 2
Bacteriophage thermal stability determination
Take potency about 108The phagocytosis body fluid 1.0mL of pfu/mL is in sterile EP tube, respectively at 27,37,47,57,67 DEG C 1h is acted in thermostat water bath, to action time after take out, carry out series 10 times dilution after counted with double-layer agar technique Number, is averaged in triplicate, observes the variation of phage titer.
Experimental result is as shown in figure 4, have result to know that bacteriophage can be resistant to 27 DEG C to 57 DEG C of temperature environment, at 27 DEG C To 57 DEG C of effect 1h, less, in 67 DEG C of effect 1h, phage titer is reduced to 0, illustrates the phagocytosis being separated to for phage titer variation Body can be with the higher temperature in tenable environment.
Embodiment 3
Evaluation of the bacteriophage to the vibrio parahaemolytious bactericidal effect of flesh of fish sample
Phage titer: 107Pfu/mL, 100 μ L；Vibrio parahaemolytious counts: 107Cfu/mL, 100 μ L.
Test is divided into test group and control group, and specific detecting step is as follows:
1) flesh of fish for removing fish-skin is cut into the fritter (about 1g) that area is 2*2cm, meat piece is crossed into flame and is then placed on one It is spare in secondary mild-natured ware；
2) 100 μ L vibrio parahaemolytious bacterium solutions are uniformly coated on meat piece surface；
3) after 15min, 100 μ L bacteriophages are added dropwise in test group flesh of fish surface, and bacteriophage is not added dropwise in control group, and 100 μ L are added PBS；
4) sample distribution prepared is placed in 25 DEG C, in 1h, 2h, 3h, 6h, 12h count vibrio parahaemolytious.
Test result is as shown in figure 5, as can be seen from the results: using the flesh of fish sample of bacteriophage processing vibrio parahaemolytious pollution Afterwards, the number of bacteria in a short time in sample substantially reduces, and illustrates that bacteriophage can kill the vibrio parahaemolytious in sample, energy It is protectant enough to play the role of food safety.
SEQUENCE LISTING
<110>Guangdong Haida Husbandry and Veterinary Institute Co., Ltd.
<120>a kind of vibrio parahaemolyticus phage and its application
<130> 2018.8.16
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 91638
<212> DNA
<213>bacteriophage
<400> 1
gctggtaccc tgctatatca aacagcaaaa cacgtagctg tataggggat tgaggattaa 60
atttcttacc ttcctgcttt tcgaactcga taactgctgg gtgtgagtaa agctcttctt 120
cagcctcaaa gattgcttca tgcagtaatt tttgcgctgc acgtaatctc ttcttagaga 180
ccggaactcc tcggtcttcc atacgcttaa ggaatcgcat acctggaatc attaagttct 240
cgtaaagctc cttcagacga gggtttttca ttacgattgg gtagaacttg ttgtgtagac 300
gtatggtagc gtccgtatct ttcgcagcgt aagctgcgag gattgggaaa ggaatcaggt 360
catatgtgaa ctgctctttc ttaatcttat gcaccttaca gtactcagac ttgaactcgt 420
ctagcttttt atcatagtta cccatatcgg tgtacttgat tgctagaggt tttaagccgt 480
gtcctttacg ttcgtccaga acgtagtgct gaatcatgga atcgtgtagg cgtttctctt 540
tgaaacactt ctcaaagctt atacccatgt gatgagtaaa ccagtgccag tcgaacttgg 600
tattgtggaa tactatatga atgtcttcac aatccactac cttctggatg aggttaaata 660
cttcttcgtc gaagtagtct gcgtttacat aaaccccttg gccttcggag tgtgacattg 720
agattcctag catctcagca tttctggcgt aaaagcctcc tgtttccgag tcgagagcaa 780
ctacgttagg acgcatttct agtacggaac gcaagtacat cttgaactca gtcatggagc 840
caataactaa gttatcttcc tgctcatcca gctcctcttg ggtagtgtgg ttacagaaga 900
tattgtagat tactttctct ggctctacgt aggttccgtc gatgtgagaa tttagacgct 960
caacagtttg gtcgaacaca ggcttgccct ccggcttaaa gaaaagcgcc gcagggttca 1020
tagaacaaag gaagttctcg taatctgacc agtctttttt aggtttagcg tctacacgca 1080
tgcctgtcat atcgactaca gaggtttcct tagaaaaata ctttaacggc tcagaaccaa 1140
ctaggataac ccagtcatac tcgtacacgt caatatcaat atcaacgtgc ttcaccaaaa 1200
ctcgttttac tgattgagtg gtaagtggaa agacatccac ttccctaccg aacaacttag 1260
aaaagttggt tttagtaggt ttaggtacaa ttagtgctat ttttgacata tatttcctac 1320
aattattatg gataatactt tcttcattta tttaaatatt atatcaataa ttagaccttg 1380
ttggctaata atttttcgat ataattctgt aactgataca cttcatcttc gtctagtccg 1440
ccagggtctt gcccaggaag taagaagtcg cttgcgtcga acaccctaca cgaggtttta 1500
gcttttatca gtttctctag cttagcggtt gctagttgcc ctgcaccatc tccatctagt 1560
aataaaacta tcctagttat tcctgatgcg gttagtggag ccagcttctc ttccgctgtc 1620
ttccaggtta agttgtgagt accaaatata gccgctgctg tgtccagtcc tttatcatgt 1680
agattaacta catcaaaaag tccctctacc agaactactg tatcactatt tttaggtata 1740
gggtaaattg ggaggttagc tgaggctggg tatactaagt actttggggt tgcattggag 1800
tgggtgtatc tgccaatcaa tgccggtata gtgccactga cggagaatat agggaaaacg 1860
accctattta ggaattctgg gtcagtgtgg gtgaaggcct ggaatttttc aaaagtctct 1920
gcctttatac ccctaaactc tttagagaaa ggtacactag attccggtat atctaaacct 1980
ctagtttcta ggagtatgtt ttgtatttgg gactccacct catagagcaa gtcataagtc 2040
ctgcttctgt actgtccgaa gtggtcgaat aagtccccct taaagccaca ggacaaacaa 2100
tgaaaacgtc ctgtgtctcg gtctattctt agcgaggggt tattgtcctc atgttctggg 2160
ttgaaacagg aaacgacata gtcgttcccc ttttcagtat attctacacc tttctcgttt 2220
aagagtttta acacgtctgt ggtcacacgt aatcctctaa gtagtatgcg ctaaggctgg 2280
tttggcgcgg ttatttttca agcatatctt ccaacaactc tttggtcaag tctacgttct 2340
ttattcttaa gcactgccaa tccataggca tagcgaaagt aacattacta atagaacgta 2400
ccttagtggc ttccaatacg actatatccg cttcttcata cgcgtgaata accatagcgt 2460
aatctgcggg gtctaggata gcttgggaaa gtcttgcttc accatcatta ttgatttggt 2520
aaggagatat cattgctacc tcatgtttac gagcaaggtt cttcaaacct tttgccacta 2580
ctaactggtc tttccaatca tacatagacg actggtcttc cacgataact tggttaacat 2640
agtcaacaac acaggccgta acctgctccc catacttaga cactatagcg cccaagtgtg 2700
catccacgga ggcgagccta agttgggaat cgtctactat aatgaagggg gtgacttctg 2760
gtagatttct tagcgatgct tcgaacccta ctaggtcaga acctttctca cacggctctt 2820
ccatttcctc tagaaaccca gatagtacct catagctgtc ctcgaacatc gctgcgcgag 2880
taactagaag tttaaatata tcagtgccag aagtttcatt attacgtaaa tccatagcgt 2940
ctgcacctga cagattagcc atgaatcttt ggaacgtctc ccctgctcgc atctcaatgg 3000
taaagtacgg gcagattttc ccatccctgt actgatttgc tgcaaagttc attgacatta 3060
ctgacttacc agaacctcgt ttacctccga gcaatagtaa atcttctcta cgtaaaccac 3120
cgtattcaaa atcccatcta gggcttatgc tagtaagcac cgtttccgcc tctgaatcct 3180
cggtggattg gaaagcagat agtttattac ctgtgaatac cttatcacta gcgtctagcg 3240
agtccgaaac gtgagtttgg atttccgcga gcctttcagt aatttcttgg gcgtttaata 3300
gagtaatgtc tttaagtagt agattctgta tcgttgttag tgatagttgt tgtgcgtact 3360
cactctctag tgcttctata gctagggaca gttccacctc atcaggcagt tctgtgtttg 3420
ctaacgcatc taaagttgct agtacgctac tggagcggtt attagctaac cgtagctccg 3480
taaggtttgg aagcttattg tatttttcgt aaaactgcgc tattaaatgg cgaattttac 3540
gataagagtc tgagaaatac tctggtttcg ctgtgtctaa taagactact ccttcctgag 3600
agtcttctcg taataaaagc attaagacta tggcttgggt gtttatattc actaattcac 3660
ccccattagg gtaatagtgt ccaatacctc ttcggcttgt tgctctgtta actctagaaa 3720
ctcggtatac ccgtcacatc cagaaggaaa gcccgtatat ttggggtact tccccaggat 3780
ttcttgctct acttccattg aattttccat agagcagtaa gctagtaacc tataagtaca 3840
tcctaacttc ctagttacgg tgtctagcct agttctcccg agtttgctag atgtaatccc 3900
tatcttataa acgtctggaa gctctagcaa cttataaaaa taaataatat ctctatgatt 3960
gttggtacac ttcctgcacc cgtggcccat taaatgggaa tgagggtttt gtaagaaatc 4020
tccgtgcttt ctacaggtta caataacttt ggttttattg tttatgtagt caaccctgtc 4080
gtagaggtat tcatttccgt gtactttttt tgcctcgctt atgaagcttt ccgtagtctt 4140
tctgtggtag tttttcgatt ggactgcaca ttccgggcaa ccttgcccct ttaaatgatt 4200
gtttggggat tttagaaata gtccatggtc agcgcaacct atgcgcacaa gctctttatt 4260
gctagagcca taaactactt cttcgtagct gtagagttct ccgaataatt ccctagcctt 4320
agctataaac actaatgctt tttctctgct gcctaagttt cttctaaccc tgccgtctga 4380
ggagagccac tcttttggga tactagagag tgataactct tctgattttt ttatcatggt 4440
ggcctcataa aagaaaaggg gcgaggctca cgccccaccc cttcgactta gaaattagat 4500
tttattactc agctgacttt tgagatgcaa ttttagcagc gcgttttgca ccatcgtggt 4560
ctgcacagct gataccacgg cgagttagtg tagctttaat accacgctct gtacggtcaa 4620
ctgctgctgc aatttccgct acagtcatat tagcaacatc tagaccttca agaatgtctt 4680
ctttagcttt tgcgctagaa gtttcttgct taggcatttc gctaatgcga ccttcttttt 4740
gtaggcttag acacttaccg cgaacgctgt taagtggtcg acccattgct tcagcaacag 4800
cttccataga agcaccacct gaaactaagt caacaatctt agcttcttcg tctacagagt 4860
aagagcgtac cggagccgct ttttcagtag gcttaacgtg ctctgttagc tgtagagaaa 4920
gaatcttacc ttgaacctgc ttagtagaga acttaccatc tagaactgct gctgcgattt 4980
cagagtaagt tagattacct gcattagcgt ttaggaagtc tacaagtact gcttcttcgt 5040
cttctgtcca ttttggctta ccagcttctg ttgctagctg cacagaatat tccattttac 5100
gaagtttaga acctactgaa cgagctgaag tacctagctg ttccgcgatt tctttaagtt 5160
ggtcttgaga cacttcatct acgccagcta ctgctgcttc tagtgctttt acgttgtctt 5220
cagtccaagt gaattttgcc atgtgtgatt ttctccaaaa attttcgggt taagagccta 5280
aaagctcttt gatagttaga attgggatgc ctaaatcgtt agctttgttt gtttttgaag 5340
atgtagaacc atcttcgacg attagggcta tagtcttttt cgttactccg ctctttacgg 5400
agtatccatg ttgttctaag aacttagcag cttctgtgcg gttcttaaag tcgttaagtt 5460
taccagtaat tactacgtct cctttggctt ccacagaggt ggacgctgta gttgactgtt 5520
ctgttacggt aaacgtaata gggatttgac ggacatcctc accttggggt gaaaccatcc 5580
agtctaataa gtttttagtg gctttctctc ctaagcctgc ctccgcacag gaggcttcag 5640
tgacgtcttc ccaagagttt gccttaatac ctagcttgtt agcagctacg gtaccaatca 5700
aagggattcc tagtgcaccg agaaactgtc cgaattccac tactcgtttg ttcgcaatct 5760
cggcgtgtaa cttagtagct gtcttctctc ctaatttctc tgttaggtag tcttccgaca 5820
aggaatacag ttcagatagg gactcaacac ctaatttttc aatggtcttc ggtccgaagc 5880
ctttaatctt catcttctta caaaagcttt caacaatttt tgctgtctga gctgggcaag 5940
aagagttacg acaaaagagt tggtctttga ctctttcgag tagagagtcg cagcttgggc 6000
aattagtagg gatgttcata tctgtccttc tatctatttt gtatactcat tatatcaaaa 6060
tgatactgag agggcaaata aaagttttcc tctttatgcc cagacctctt taatcaattc 6120
gcttgtgtcc caaagcatga gatatattat atgctaagga ttcattcgcg tcaatggtac 6180
tttttaggag ctgactgaat agttgattct tccgtcaatt ccacaactgg agcgttatta 6240
atgatagcca gtagcgtatc taaggatgtg gtcactttgt ttttatatgt ggtcagtgcc 6300
ttagttagtt ctaacacatc attttcagac aacggcttct ctgctaaaga gaacgtggtg 6360
atttttgcta ccatttgcaa ggttgcatcc gcctccgcgc cgtcaccacc taacgcaatg 6420
atgagagtca gtcggtcggc ttctgtggtt tttattaact ctgataggtc gtagttcttc 6480
atttaagttc cttgtaggag tgctattcgg tcatcgagta tacttacata ctccatcatt 6540
actagtcttt gctgtactag tagttctagc tggtcggcta gttcttttgt tacttcatgt 6600
cctgcgacag agtccaagaa tgcctccagc cgatggagct ttataaataa ctcgttacgc 6660
tctacttgta attccgcagc tcttagtttg ttacttttca tattacctta cgtctataat 6720
cttagggata atgccgcctg agcggataac acaaacctcg cagccttcgt agactcctag 6780
gttttccaca taaccagcat tgtttaatgt tgctcgggtg atttctgcat cgtcgataaa 6840
gattggctca aagattgcta ctggagtcac tttaccagac ttaccagtct gccatgttag 6900
gtctactaac ttagtccaga taccttcttc gtcttctttt acggcgaatg cacctttagg 6960
atgtttgtgg gtaaatccag catcgaagaa atctctgttg ctagagaggc ggaacactcg 7020
cccgtcttgc gggcagtctt cccagtctga gtcaactacc gtacagaaac cttcaccaga 7080
tagccaggcc atatcctgct catagtgagg gaaaatacct gcgtggtctt ccgcttcctg 7140
aatcccataa gctacgaaga taagcccgcc ttctaaaaca cgctctcgaa actctttttc 7200
gtcttttagc tgcatagctc cagacgcgta gttacgcatg ttttttacag gattggtagt 7260
aactacctca ccagtaattt ggataggaga gctactagat agttcacctg gtacaagcaa 7320
cgccagcttc cacggctcaa aagtggttgg ggatgttatt ccgtcgccac gagtgttacc 7380
ttgaactagc ttattaccaa tgtaaaacaa agaaattgca catccgtcca atttcggagt 7440
ttctatgcat ttttctacag gtagtggtaa ttcctcacct cgaccaggat acaccttgtc 7500
tagtgagtac atacgataca tatgcttgaa ttcgccgccc acacccaatc catcctctcc 7560
aaagcgagag actagtgcat cgtactcctc attagagatt actgggttgc cttcgtagta 7620
gcacttttgt aggtaacgga tatactcttg cattacttgt cctcgtcttg ggtagtatgt 7680
ttatggtgat tttctattac ttcttgagat acctcagctt gagccatgat agaggcaaaa 7740
gctgagtata gtctagttac tgaatctagg ttataaggga agcttagtcc attccgtgta 7800
ggaagccact ccccttcaaa gtctaggtag tactccctaa tgcctaggta gtgcctaccc 7860
atgaattcag aaacagtgaa gcgtaactgg atgcctaatt cttcatcttg gtagatgact 7920
gcatccagct tatcttcatg cccttgtaaa tcgtcgtgaa tcacgttctt gtttgtctaa 7980
ctcataactc agcatggaaa gatgagtaaa cacgtccaca ggcattgtac ggaacttgtt 8040
agttgtgtaa cagaacagag ccacgtaacc atctttaggg tttgccacct gcttttgtac 8100
atgctcagga gctttctcta gctctgcttt caggtacggc gtatcgaagt tagtcgtagc 8160
cacgatgaaa gacttgttgg gagtaccagc tttcttatac ttatattgag catagaaagt 8220
atcgaatttt tgggataact ctaggaagcg ggtcattcgc atatactact ctccttatgc 8280
ttgtgactga agttcgcgga tttttgcagc taggtgtgat gctgctttac cggttagttt 8340
tgagataatg tcgtcatcta ggtcagtgac tccaccatct ttaaaggctg cgagtagctc 8400
gccttgggct tctgctttgc ccacacgctt agaaccacct tctgacgcat tagatgtttt 8460
cttagctttg ttaatttgct taacatacac acctagttta gttagctgtg cacgagctga 8520
tgctacagat gtaccaatct cagctgctgc gtaagtaagt gcttcgttag agaaagtacc 8580
ttcttccgct tctgggttag cttccgctag ttcacccatt tttgctttgt atagagattc 8640
tacttctgct ttaagctcat ctgtccaaga gataggtttg gtcataaata ttacttcctt 8700
tggttgtcgt ttgtcgaatt tatagatata ttatacgtaa ctttcagcca ctaagcaact 8760
gaaagttttt gttattggga gttacgctat gtgaaagtga ggagccaggt aggcctcact 8820
ttcctttgtt agcgttctta cgtgcagcca gcaacgccgc atttagagta tacacaatgt 8880
cctctctaga catattgtct tggagaacgt tagggttgat acctttttct tctaggtatt 8940
ttaagctgcc taaatcgtat gcgtaaaagt ttacgttacg atgtttattt gatgctagca 9000
ggtacactcg ataagcatag ataccgtcgg cattaggctt atgttccgcc ataacttcac 9060
ctagacattt gtaccctgga acccatactt tttgcccaat agcgaaacgc tctgacatgg 9120
cttcctctgg aaccatagca gggtttagag ggtctgctga ctctctaagc ttaaagtcta 9180
aaagacccat aagctctagg cgacgacgca ctaaggctgg ggagcggtag tatcgcttgg 9240
caatttcttc aatagcatca ccagtaagat acgcttcagc catgttaatt tcttcctgtg 9300
gagaaagagg cttagtacgg ttcttgcgca tcatttcttt gcgatgttct ttctctgcta 9360
aatagttatc tagcatttcg tctaggcgtt tggtattgta gttaatacct aggatttcac 9420
attgacgttt cttagtggca ccctgttcac gctcagcaag agccatttca aattggtctt 9480
ctgttacgtc tgctgctgat tttgaatttt tacgagccat tagtcgtctc ctagttctag 9540
ttctaccatg ttaactccgt accagtgtaa ggttacgggt atgtcaatgt accttagggc 9600
tttcaataac agaggtagcc gtgtggccaa catagttgaa ttatacccat cgaggagtac 9660
gttacgtcct cttatataat tgtatttaca aaaagagaaa tcagccttta cgtctctaat 9720
aatcgacttg gctacttttt cctttctctt gttgtgcttg gctcttaatc tatgaatctt 9780
cataagctct ccaattaatt tatacaaata ttataagata aattggctat ttcggcaatt 9840
gaagattttc ttttcttaac tccgttatta gacgtttacg tttaagtaag agggcggata 9900
agtcttcgtt cttttgtatc cggtatacga ttgggaattg aagtatgata tccgccccta 9960
aaagtgctat agataagggg aatacacgac acccttgggg ctgaaatctt ttatacttct 10020
ttttcttttt agggcgttcc ttgcccatag ttatactcgt gaattcctta gccatactgt 10080
accgttatgg aaaagcctgt acttgttatc acgtttctac agtccattag ccttccttct 10140
ataggaacca cgtaaacttt tgcggacacc cctaagtatc ttaaaatttg tcggacatat 10200
ttaatatccg actcactata catagtgtac ctgccaacta agaactttac cttttcttcg 10260
cctagagcta gcactatctc cctatacgcc cgcttagcag cttctacctg cctagacttt 10320
tctagattcc tattttcgtc tatgcgtctt cttatctttc tagatatcat gagaacccct 10380
taacgtttac ccagcttagg cttccgtagt catcgctagc ttcttcctct cgctgtagtt 10440
tatgattcaa atcactagct gcttccgcta gtttagcttt ctcttctgcg gtcaagaact 10500
ctttagggat tgctaactct tcatactcgt agtacttacg agcccagtca tgcaactgct 10560
cccaattagg ctccacgcct ttgtcttcta tatcaataac taaccctatg cggctagcct 10620
gatatttagc gttacgcatg ttcatttttc ttccgtaacc tgtgaaggcg ttctcgcctg 10680
tggcattata ccattgaata gcgtgaacac ggaggaactt agtaaaacgt tccaatgtgc 10740
ttgcgttgac cttcatcaga ccgtacagac ccccacgtac tacttcagct aagttagcca 10800
tcttggtatg aatatcttgg tcaatataca tgtctacgtg gtcgtacgag atagcatcaa 10860
cactaggaag ctcgtaatag tgtgcgattt gttgttttac ttcgaaagca ttgttatagc 10920
gtcttgggaa gtggtcttct ctagacatta agatagcgta aatcatcgct tcacgctccg 10980
cagctttcgc accttctcca cacatgtcct taacagtggt ctctagagtc tcccctggcc 11040
ctgcctcaaa caagtcatcc acaactactt cacacccttg gtccattagc atgaatcgta 11100
ggtttgactt aaatccagag aactctgcat ttttacgaag tgcgaatttt tcacgcatta 11160
agtcttccag acttgggtac gtgttccccg cacggaagta ccctgtagag aagttagaag 11220
tatctgggtc tgtgtaaatc cagtactctt tattaggttt gcgacaacgt cgcaaggcct 11280
ggaatcttag gttaggcggt gcaatctttg tgttagtaat catagctaca acatcaaagt 11340
aatggaagtc tactcccgat acgacggatg gagaacacac taaacaatca acgttgtact 11400
gagctaacgc agcgtttgtg ttctctagaa tatctcgtat atcttcgtct tttgtagact 11460
ctgaatgcac acccttgatt acgtggccag gatgtaacat ttctagtcca cgaactcttt 11520
cattgattgc cttcggccct aggtctgata ccactagaac tttcaagcct tcgtctaggt 11580
ttgcgtgaac tcctgataac catacggttt cttctttctt acactcgtaa gctttgctgc 11640
cttctagctt gttaaatttg taatcaactg ctgctacttc cttatccgag agtgcaaagt 11700
atgagaacat ggtttcttca ctaatgtcac catctgctag aaccacatac ttagctctgc 11760
gaagaatagt actaagagcg tcaatacact tagccctttc tttcataata accgctgatg 11820
cgttaagcaa agtatccatt acagcatcgc attcatcaat gaatacaaag tctatcgcac 11880
cttcctcagc catttcctta aatctccaca aagagtggat tgtagtagac atacgtccat 11940
tagggttctg gcggaaagaa tctcggtgag cagggtctaa gtacgcctta tcagttgggg 12000
cacccaactt ggcggcgttg ccatctacta gagatacggt gttagttact gctaacatac 12060
gcccatgaaa ctttggcttg atgcctaaaa tctcgtcacc taaaactaga cgttgaagtg 12120
ccgtagtctt accagttccg agggatgcct tccagaacgt aagtttgccc accgggggca 12180
tgtctgggat gtacaggtag ccgttatccg ttcctgcacc gtcaggatac agtttctcta 12240
ctggccaatc agttaagatg tcttttcgaa cctccggagg gatttcctca tatggtttga 12300
ttcgaatgtc tatcttatct ggaatggtgc gtatggcgcg agatttgatt gtttggaaac 12360
agaattccat ggccgctttt aagtagtcgt cgatgcttct ctcccctgag acaatcttag 12420
ggtggtcttg catagcaata gtaaatgctt cttttagcgg ctcgaatcct ataccatcct 12480
ccattgccaa gaagaatagt cgaggtgctt ttttggtata gacgttatca gggttttcct 12540
cgatgtcgtc tagcactcga cggactggag gtgtaattat gtctgtgcct tggaaccttt 12600
taatgctctc taggtcttct tcggttagtg ttgctgtttt cacacgctcc ttctcctcag 12660
tagttggtgg tattgatgcc gtaactcttg atttgtagaa tactgttcgt ggcatcacac 12720
caaaagggtc ttccatgcaa ccctcaaata tagggtctgc cgtgtacagt atgtgggcac 12780
gagtgtaaag agagtcatcg aataagtcgc atttgaattc ttcgttaaag acttcgttta 12840
cctcgtcagc tatagtgtct agctgctttg tatttacagg aaccgtgttc tccaagtaca 12900
agtgcattcg gatggtgtct gtcttaatac ctgcggaact agagccccta gcaatgtatc 12960
cacacttctt atcgaagtat acagggaacc gttctgacag tagttctagg acatactttg 13020
cctgttggtg gatatctaga gggtctatgt gctctggcct aggaaaacta tcaatatcga 13080
ataccatgaa tttgctcttt ggtactacac tgaaagtttc cttgttacgg cgaatgtttt 13140
ttcgtggtgc tccttccgct agagcatgct gtagcataca aacattaggg tcttgggagt 13200
actttagtac aagtgtgcgt aactcctcca tacttgcagg agttacttct tccacgacat 13260
caaaatgctt tgctaaataa atatctgttg tttttgtagg ggagtgccat accttggtca 13320
ggtacttttt acgattttga ccccatccgt aggatgaagc ttctggagcg gtttcgttct 13380
ctacctgtgc tataaaggta aacacgtgcg aaatcctcca tactgtagcg agatacgctc 13440
atggatattc tctttaataa ctatactcct atggtggtta attttttgcc actatattat 13500
tttttatata ctcgcgagat ttcgagtatc tcacagaatt tggacgtaat aatgctcggc 13560
tgagctagac aagagtcaag aagctcgggg tagcaaaatt tattctgtgt taacacttta 13620
cgattgtaaa tctcaggtct cgcctcaccg acgtcccacc cgacagaagt taactgactc 13680
acacaatccg aggattgagg ctacaaagtg atagtcttgc atttaagcag ggagttacgt 13740
acttggtgat aaagtccttt acaagccaca cgtcgacaga agttaactga ctcacacaat 13800
ccgaggattg aggctacaaa gtgatagtcg cgccttttaa gacatagtgc tgctaagact 13860
tactttctta aagtattctt aaatgctcag aaccgaacat ttaagaatac cctaattaaa 13920
gggtatatag gagttctctt attaaagaag taccagcgta cttgagggaa aggttgtcca 13980
gaccttttag agaatagata tatagtatac taaaggaaaa gaaaaacgtc aactctattt 14040
tttatttacg agtccacatt cctagttcaa ctagtttatt tcgtctaagt tcgatgtaat 14100
gcaacctttc ttctgctcgc atatcaggag taattaagac acctttgctg cgttgaatag 14160
ttacaggaaa gtacggcttc aatagtaact tttgcacttc cctcttttgc cactcactta 14220
actcttggta gaaaggagaa gagaagtgat ggttatggat actgataagt ttatgcagcg 14280
cccaagcatc tttcccaggc ctatggacag ccacagaaaa cccctggttg ctacatacca 14340
gagccattcc caaagctacg gcagtatctg ttagtttctt aatctggtgg tgtactttta 14400
catggtagtt agtccaaatc atgtgcgctc tctttggtta tttttcgaat atacgcatat 14460
tatacaaaat aaccggctcc ctggctacta aatttttacg gaatcctcct cgcgcgcacg 14520
cgtaaaatta gtaatgatat ctaataccct atcttcctcc ttcatggcaa gtaaaatttt 14580
taaaatcgct ctaataacca ccacaactgt aggttagtga gctttagaaa attttacttg 14640
catctatgcc ccatatttga tatgatatag tctaagttga aaagctctat tacgagaaaa 14700
aggaaactaa atgtcacaaa aactatatat actagatacc aacgtactac tttctgaccc 14760
tcatgctgtt cttaagtttg aggagcataa cgtagcaatc ccaatgactg tactagagga 14820
gctagaccga cagaagtcaa gtgacagaga tgtagcccgc gacgcccgag ctgtcatccg 14880
taccctagac tcaatcatcc gagatatgga tgctattgag gacggcgttg aaatgaactc 14940
aggtggacga ctattcgttt tacctaacat cggtgtccct gaagccatta aagttacttg 15000
tccggacgat gcaattatca atgcaacgat tcatcagcaa gaaccagccg aaggctcaga 15060
agctttcaag ggctcacctg ttctcgtaac taacgatgtt tgcatgcgct taaaggctag 15120
gggcgcaggc gtacaacaag ttcaagagta tcgtaatgat gtagtggttc aagaccctga 15180
cctattacct cgtggttact ttgaagtgcc agacggctgg ctaagcactg taggtaacga 15240
tgacgttatc tcaaaatctt gcggtcgcac cctaattaaa acctcagtaa tcgaagaatt 15300
actgggagat gaacctttcg gtatcaacga ttggttaatc aacgaggaag acggtatctt 15360
cgctcagcta gaggggcctg tagagggcga ttctcgctac gaagatggta tgtacctcga 15420
atttactttc gtaaacgctg gacaattcct gaaccgcaag gcttctggta ttgttcctaa 15480
agacttttac caagccattg ccctagacgc cctactagac cctgaactag acattgtgat 15540
tattaacggt gccgcaggtt ctggtaagac tttgctagct atggcagcag cagtagagat 15600
ggttaaagga aaacgtggct accgtcacta cgacgttatc gtttcccgaa gcatgcactc 15660
tcagttcgac gatattggtt tcttacctgg taatgagtct gagaagacca aaccgtggtt 15720
aagtggtgtt actgataact tagagcacct ggcacgagta accaagaaca tgaagctaca 15780
ccctaataac tccattgtta gctcaactga tactccaact gacgagcaag gtggtgatgc 15840
gttcatccaa gctaaagcgc tgaactttat gcgtggccgt tctatcgttc acaaagtctt 15900
cattatagac gaaattcaaa acctaactca gtcacaagtt aaaaccataa tctcccgtgc 15960
gggtgagtac tgtaaggttg tcctaatggg ctccctaaat cagattgact ctccaattgt 16020
aaccccaaga acttcggcac tagcctgtac taccgaaaaa ttccatggag aaccgttcgc 16080
caaggtgatt acactaggtg gagtacaccg ctctcgccta gctaaatttg ctgacgagca 16140
cctttaaaac taaccccgcc tagtgcgggg ttttattctt caaaaattca tttgcggata 16200
ttcccaacat tttgtataat actttccaga aattgagaga ggagcattaa ttgatttcaa 16260
cagtagtaaa gcacgacgga cgaacagaac cattctccgc aaccaaactt aaccgcctag 16320
cagaatacgc agcggtagac ccgactatga tgtcggatat ctcccttaac acgctaaaga 16380
acttatatga cggtgtgtca actcaagaaa tcctagagag tatgattcgt tactgtcttc 16440
taaaagaaga tttagcatat actcgtgctg ccgcacgcct agaagaagct attatcctaa 16500
aaaacgccga acgactaggt attgatggag tagatgactt caaaggttcc atggagcagc 16560
taattgaact tggtttatgg tgtgaagtaa cactacctaa atataaccct gagtgggaga 16620
attggctaat cgaactatcc aatatccctc tagagtactg gactattaag caatggtctg 16680
ataaatactg taccaaatac gaaggcttgt gtgtagagcc agttgcttta ggtgccatcg 16740
gcatcggttt agcacatcat ggagacacgc agctagctct ggatactgca cgagcaattg 16800
tacgaggaaa gattaaccta ccaacgcctg ccttaaacgg tggtcgtaca ggtgactttg 16860
actccgtttc ttgctgtgta atttccggtg gtgacaacac acactcacta ggtgtagcag 16920
accatatcgc gtatgaaatg acagcgaaaa aagcaggtat cggtatcgag ctagacactc 16980
gtagtaaagg tgcccctgtc aagaacggtc gagtagagca cttaggcaag cactctctgt 17040
acaaacgaat tgattcggct gttaaggcat tgacgcaaca aacccgtggt ggttcagcaa 17100
cagtatctta caagtgtatt gacccagaga ttcagaaact tcttcaatgg aagacacaga 17160
aagtggacat tgaggaacgt attgataaac tggactatag cttctgtatt aatgatgcgt 17220
tcatggaagc tgtaatagct aagcgagagt ggaagctgta cgacctacta gaagcgccta 17280
agatgcacga gctattcttc tcgaaagaga aagtaactct agcggaatgg gaagcagaag 17340
gctctaaata taagagtgca ggctcagtag atgccttatc tctacttaag cagttcctaa 17400
cagcacgcca agagactgga cgtgtatact gttttaatgc ttcacgagct aactcgcata 17460
caccttttga agaccctatt cgtctatcaa acctatgtgt tgctccagag acccctatcc 17520
taacgcgaaa cggttacgta cctatagcgg agctagaggg ctctaaggta gaggtgtgga 17580
acggagaaga gttcactact acaactgtaa ggaagacagg aacaagccaa aaactagtac 17640
gtgtagtaac agattcggga caagagcttg agtgtacaga gtaccacaag ttctacgtac 17700
aggtagggca tggtaagggt aaggtagtag agaagcgtgc ccacgaacta caggcgggtg 17760
acaagcttat taagttcgac ctaccggtta ttgagggtag cgaggattta cctctagcat 17820
accaaaacgg gttcttctct ggcgatggtt gccaagtagg aaaagataga cgcgtttacc 17880
tatacgggga gaagcgagac ctcctaggta agtttagctt tactggactt caagaacaac 17940
cggaacaaaa tcgagtctac gggaacgtag caggcctacg agacaagttt ttcgtaccag 18000
acaatactta ctctattgaa tctcgtcttc aatggttagc tggctacatg gatgctgatg 18060
gttgcatcta taggaacggt acaaacgaac agctagtggc cgtatcggta gagaaagagt 18120
tcctaaaaga cgtacagtta atgttacaaa ctttaggtgt ggactcaaaa gtagttaaac 18180
acatgaaaag tggcaagcaa ttgctaccgc taaacgatgg ctcaggtaac gtagggtact 18240
tcaactgtaa ggagtcgtac cgcctaatcg ttacatctaa tgggttattt aagttatctg 18300
agttagggtt ctctacagag cgcctgagtt ggaaacctag aaaaccacaa cgagacgcta 18360
agcgtttcat taaggtagta gaagtattag acgaaggtcg ggtggacgac actttctgct 18420
tcacggaaga gaagcgtggt atgggtatgt tcgccggtat tctgacaggg caatgtcaag 18480
aaatcttcct acctactaaa ccatatgtag atatgctaga cttatacgta ggtgacgaga 18540
atggtgattc tattggtgag acagcattct gtagcttatc agcaatcaac tatgctaacg 18600
ttgaagaaca tgaaatcgaa catatcgtgg aagtagcgtt acgcaccgtg gatagcatga 18660
ttgaaaaagc acctggcctt actgagccta tgaagaagca gatgctaaag cgtcgttctg 18720
taggtatcgg tatcactggc ctagcacagt tcctgtataa gaatggcctg gactacgatg 18780
gctctccaga atctctggaa gctgtgtctc gtctagcaga acagcacatg ttcttcctgt 18840
ataaagcatc tatcaagatg gctaaagagg caggagagtc tgtaacagga attactgatt 18900
ggctgcctat cgacacacga gtcaataaga agtacgagcc tatcatgtca tgggagtcga 18960
ttcgcggtct tcctcgcaga cactcagttc tagtagcaca catgcctaca gaatctagtg 19020
ctgtgtttag tggtgcgtca aatggcctat accctcctcg tgagcgtgta atctacaaga 19080
aagcacgaac aggtaacgta cagttcattg ttgaccaaga gaacttcttg acagcttggg 19140
aagtgcctaa tactcgccac atgcagtact acagccgagt gcaggactgg acagaccaag 19200
gtatctcagc ggatacttac gtagttcctg agaactaccc taatggtaag gttcctatgt 19260
ctgagctaat caaacaaatc atttacttct ggaagtcagg aactaaatcc ctgtactacc 19320
aaaacactaa agacaaaaca tcaaaaacag cgcaggatgc tatagcggca gctaatgccg 19380
aggtagacga gggctgtgaa ggaggttgta aactataatg tgttttccca catatactcc 19440
gtatattcca gaacataaag aagtatacct ggcagctcgc gctgccgggc ttactaagtt 19500
agatgcagga aagttgctaa agagcacttt cggtctcacc tctttcctag acgctaaacg 19560
tttagtacat attattgaag gagaactaag ttgagtaagt tcaaccctaa caacacaggc 19620
tataagaagc aaaaatacaa actcttccta gacgaacaaa tgggcttacc ggatactgta 19680
aacgtaacct accctgaact agaggagctg taccaagacc aggtagccca aatctggaac 19740
gagtttgaag ttagcctaac tcaagaccgc atggacatgc aaaaggcacc acaaggccta 19800
gtacgtccta tgcaacgcac gattagttgg caaatgctag ccgacgcagt agcggctcgt 19860
tccatcggag cactattact taagcacgta acgaaccccg agctagaaaa cctaattaac 19920
ctctgggcgt tctttgagac tatccataac cgaacatacg cacatatcgt aaagcaaacc 19980
ttccaagacc caacggaaat gatgcaggaa acttacgaga atgcgcaggt tatcatgcgt 20040
tctcaaaaca tcgtaaaagc attcgatgct ttagaagacc tgccgaaaga ggctagtgag 20100
cgagaagtga aacgtgcgct attacgcgca ttagtagcat tgtttgcctt agaagctatt 20160
tctttcatgg gttcttttgc tgttactttc gccatcacgg aagctaaagt cttccaaggt 20220
attgggcagc tagtaactct tatctgtcgt gatgagcaac tacatacacg attcgattac 20280
gagattctga acattcttct ttacaaagag ggctgggcgg cagaagcagc tcttatgaca 20340
gaagagctga aagcaatcct ggatgaagta gttcagcaag aactagattg ggcatcttat 20400
atctttgaag aggataacat ggtagggctt aatgagaagc tactacagtc ttacatccta 20460
tacatggctc gtcctgtgta cgaagcacta ggcatcccgt ttgactttga agttattaaa 20520
gaaaacccac taccttacat gaccaagtac attgactcta gtaagatgca ggcagcagct 20580
caagaaatcc aaattacgtc ctaccaaatc ggggcaattt cggatgacag tgacgacctt 20640
gacttcggag acatggactt cgacgctgat ttcgaataat tacaatagcc aattcaattt 20700
gaattggctt ataatatatt tttaattgat ggagaaaccc tatggacaaa gtacttgacc 20760
tacgagaagt tgacttagct agcctaacta aacaacaaat ttgttctcgc attcatctaa 20820
tccaagatga gcagcgacgc ctagagaaag agcatcgaaa tcttgtccgt atggaggccg 20880
cactaactca ggaactatat gaccgcaatg attaaactgg ctgtagccac aggagaacgg 20940
ggcgagtttg ggttaaaggg agggctgcct tggggtaagc ccctgtccgc agacatgaag 21000
gaatttcgta ggtttacgga agactgtaca ctacttatgg gtagcgcaac attcgaatca 21060
ctacccacta aactacctgg cagaaaacac gtagtattgt ctagtaggga agttcaggcg 21120
aaggacggtt caaaaccaga cttaatcctg aacgaagcaa ttaccgagct gttactacat 21180
tctattgtgt ttctatttga taggtgtgta tgtgttatcg gaggtgcagg agtagtggaa 21240
acatgcgctc cttttgccca agaaatacta catacctaca taaaaccttt aggcatccat 21300
gcaatgagcg ctgatgtcta tgtagacaca tcaaagttgt attaccaccc aactaggaag 21360
ttagaagtga tatccgcagg ctgtgaaggg gattaccact actgcgtttc catccttagg 21420
agagaaaatt gaaaggcaca caaaaactat tagaacttgg taacaaaatc ctgacggaag 21480
gcgagtggat ttacaatgag agaaccggca agaactgcct aactatcccc atccatatag 21540
ctacctacga tgtaggtgct ggcgattttc cgatagatac cacccgtaaa caagcatacc 21600
gtggccctat catggagctg ctagggtatg tacggggttt aacaaacgca caagattttg 21660
cggatatagg tgccccaacc tggttctcta atgccaacaa aacaaaggct tggctaaata 21720
acccccatag gaagggagag aacgattgtg gacaaatcta tggagcagta gctcaggctg 21780
actttgtaaa ggtatacaag aacctgaagg ctggtatcga cgaccgaggc gaaatcatta 21840
ccttctggcg tccagaaact tttgcaaaag cttgtctacg accttgtatg cacacccata 21900
gtttccagct aataaatgga actctgcata tgactagtat ccagcgaagt gcagatgtac 21960
caatggcggg gtcgtggaac gctattcagt gctacgtact cctagcacta atggcacaga 22020
taactggaca taagccagga aaagttacgc atataattaa taatgcacac atttatgaag 22080
accagttaga gctgtttgaa gaacaaatgt gccgcattag tatgttctgt aagcctaaac 22140
taaccatcaa tcctgagatt aagactttag aagacctaca aacttgggta actaaggaag 22200
acttcactct agaaggttac acctctcacg agagcataac atacccgttc tcagaataat 22260
tttaattgct aaaaagccct caattccgta taatatactt atattctgga attgagggct 22320
tttctatggc tgaactaaaa actatatcta tacatgcttt gatactagag tactctggtg 22380
ttgcggaaaa ggagcctagt tatagactag gacagcattt cataaataga tgtgtaaggg 22440
atgacaaaga ccccttactg gcagggttgt gggaggaaac cgacccagag atggccagca 22500
tacagatttt gaaagtgtgc gatgcttggc aatgggattt gcgaaatctt cctgtagaaa 22560
gagattggag cgtataatgt attatgtatt tactgacgga ggggctcgtg gtaacggcaa 22620
gggtgtctcc gcttgggcaa tggtggttta cgacaagaac gaggttcgtg taggctctaa 22680
atctgagggc tttggctcaa gctttggata taccaataac ttcaccgagc taacggcagt 22740
attgagagcg ttagagtggg ctaagaaagc caaagttaaa gtgtctatcc gcacagattc 22800
taattatgta aaacagggaa tcaacgaatg gatgcccaag tggataaaga atcactggag 22860
aacctcggcc aacaaaccag ttaaaaacgt agagctatgg cgacaggtgt accacctatg 22920
taaagagtta ggggatatgg tagaagttat tcatgtccca ggacacgcaa caggcaatag 22980
ttttaacgcg gttggcaaca gggcagcgga cgctctagcc aatctagcta tggatgaggc 23040
tgaatttcag gaatatctag ataaacatta aaaattacag ttgctaatta gatacatttt 23100
gcgtataata tacttctaat ttgaaaggag atactatgcg caattttgta gctaagcacg 23160
cacgctcctt taataaggca gcagtgcatc gtgatagaac taagtacacc cgcaatacta 23220
actggatgga cgaatggctc gacgatatcg agcctcccag agttctaata taagtactat 23280
tatttatatt agcattctaa caacacagga gaccatatgc tagcacctaa tctacatcaa 23340
cacctaggat ggatggctga cctagagaca ctggctaccg ctaactcagc agtagttccg 23400
gaaatttcac tagtagcttt cgaaatcgac actggtaaaa ttgtacatga aatgacccta 23460
cacctttccg tagatgaaca gttgcaggca ggtcgaacgg tatctagcgg tactctaggt 23520
ttctggttaa ctcagacaca agaagcccgc gataagttac ttctagctat ggacgattac 23580
caaaaccaaa caatcggtaa acttccgtgg ggagtaaacg aagctctgac ccatatcagg 23640
gatacggtac gtatgattac ctatcaatgg gaaattcaga ataaacacaa aatagaccgc 23700
gatattgatg agtccggccc acgccctcta atctatggca atggtgctcg cttcgattgc 23760
ggtaaggtag cggatttgta tgagagagcg aatcgagaaa atgattaccc ttggggttac 23820
agtggcgatc gtgatattcg tactttgcat tctctagccc cgtcctttaa aggacaggaa 23880
gagtttgaag gtatcccgca ctttggttta gatgactgca agcatcaaat caaatacaca 23940
cacaaaattt acaatgcaat tttggaggcc tcagccctac aaagttgatt tcttcagttg 24000
ctgaatagct cgaataaacg tataatagtt tcataaatta aaaaacgaca aagaggaaag 24060
attatgtcta acactaataa agtagtaaaa gcacctgtag ttggcgaaac tgtaaaggta 24120
aacctataca ccgcgcgaaa tgcgaaagcg ccaactgatt acacgggtcg cctacagcga 24180
agccgctcct cttccatcga gcgcatctca gcgatttact ctgatacaga gaaagacaaa 24240
aaaggcaata cactctacag cgttctccta acttctggag accaagtgct aatcgctcgt 24300
ggcgataagg gttggtacgc tgttcgttaa accccagtta agggcgaata attaaaaact 24360
ttatttgccc aacctcttaa attttcgtat aataatatct taatcagaaa acaaggaaac 24420
accatgcaaa agatgtcgcg ccgagggaag atgttctcta accatgacta cgcagcgaaa 24480
gtaatgacta ctcgcttcaa gggtctacaa aaaatcctag cgttgctttc acgaggaact 24540
tcggtttcta agtcagaagc agcaatctat cacgcagcta accaaaaggg gctattctaa 24600
tatggcatca ggtcgtaatt caaaacttat tcgtacgcta gccttactaa actctagcga 24660
atcaggtcgt atcctagaaa ctaaaatggt ctatggtggc ctacgcacta ctcagcgtga 24720
ctacgtacaa aacaaaggtg aagaaaacga gtttactgtt agcactcgta cccttcaaca 24780
cgtagagggc acgtataaga gcgaagttaa gcgtgcaaaa gcactatacc gagaaaaagg 24840
tgtagacggt cttattgaag cgattgacga ggcactttcc cgccacattg attaaatctt 24900
caatagcttt caaggtaaaa tcttgatata ataattacag aaattgagag aaacaacctc 24960
tcaatttcta attgaggtgt agctcagtag gtcagagctg gggaatcata atccctgagt 25020
cggcggttcg agtccgccta cctcaaccaa ataatttaaa attacgtttg cccaatctct 25080
ttgagtatta tataatattc aaacaaattg gaaaacaaag ctaaggagct tattatgcca 25140
tgcagaagct acgaaccaat cgacgtagag cgagcggata aagagtccgc ccaacaagaa 25200
gtacgtcgtc taaaaaatag tctaggtaaa gcccatactc atatggataa cctgacacgt 25260
atgctttgct atgtagttac cagagctatc gaggaagatg tagacacaga catcctaaat 25320
aacgaagtgc gtgactggta cacagcacat cgccaagctg accttgctaa catgcgtaag 25380
ctaatggaaa acgttgattt agacagtctt ccgaaagaag aattcgatac agtcgaccga 25440
attttgagca agtaccaaaa taaataaaaa atcattagct caatagtcga aaagtttgat 25500
ataataatca aacaaattga gaaaagaata aacgcgggtg taggtaggtg tggtcacctt 25560
tccagtctca taagctggag tacagggttc gattccctct cccgcatcca ttttggttct 25620
gcatgcccca agaatatatg caggtaaaac ctaggatacg gtgcctagga acccaattac 25680
attcaggtga actgcgaaac tttcgactac catcgttcgc gcagaagttt accgaatatc 25740
gcttatgggc gaggcgatta ataaatatac ccatgcataa tttatactga gcactccata 25800
cgtcgctagc tcaatttggt cagtagcacc tggcttttaa ccagtaggtt ccgagttcaa 25860
gtctcgggcg gcgtaccaat ttaaattgag gtgttatgaa aacacagaaa aataatcggg 25920
acattagagt caaggagctt cttgactggg ttcacaaaaa taaactgttt cattctatct 25980
caagaaaacc tagcttgata gtcaacgggt acgcaaagtt tgttactaga gctaaatgca 26040
ggaagaaata cggtaaagta gacaagtccc tacaatatta caaaaagcag ttagagaaac 26100
agaaggtatt ttccttaatg acactaaata tcctctgtaa gttacactcc atacccaatc 26160
acataggtta cgtgtatata gtgtcaaacc cagcgtaccc tggatggtat aaagtaggtg 26220
cggcaaagga tgcggaaaca agactactta actaccagac aggaaccccg tttcgggact 26280
ttaaactaga atggtatcta ccctgcatag actatgtagc tagagaaaaa gactttcata 26340
gtaacagctc tttctcacat gagtgggtac agatgccctt agatgaatta aaacaaatgc 26400
tcggttcgac tatcggttag gtcatcaggt tttcaacctg agagggcggg ttcgactccc 26460
gcaccgagta ccatattcta aagaggtcta cttagatttc tttagttaac attctgaact 26520
cgtgccagcg ctagaaaaca cgcccgaaga cagggagatg acaaggtggc ggatgtcttg 26580
gagatagcaa ctccgatggt gtaaaagagg gctttgagat accttgtgaa acccacgaga 26640
gtaacgcgga gcttagggtg ttaagctaaa gaaatttaaa aaatcaattg cctgagacac 26700
aggaaattga tataataatc aaacaaattg agatttcttc gagaaagcga acaaagacga 26760
tagcagctaa atgtgggtat aacactgctg aaatcctcag ggcaccctca acgcgcaaac 26820
ctgtatgcga aagagtcgga gttcacgttg tctgtaaccg gtttggacga cagctttcat 26880
caaagagatt tcggggtctt agctcagttg ggagagcagg gtctttgcaa ggctcaggtc 26940
gagggttcga gtccctcaga ttccaccaaa ctatctgcta gtacataaac caatagaact 27000
acggttccgc cttaatgtaa ggtttaagat agagtagtac tggagaggca aagtagatgc 27060
gacagattct actaaaatct tctgccatgc caggagtacc aaattaccgt cgtaataaac 27120
gtctatggag gacggcggga ttgtagaccc cgtgtcacga aagtggctcc ctaggttcga 27180
ttcctagttg cggcaccaaa tttggggaca taactcagtt ggttagagtg gcatcctgtt 27240
aagttgctgg tcggtggttc gagtccacct gtctccgcca aattacgaaa actttcaata 27300
gcctaacgag tttattattg ctataataac tttagaaatt gagagaaaag gaaaacttat 27360
gtttaatcag tttatggtaa ttagttctat gggttggatt atccctaaag gtggtaaaat 27420
gaaagcagct ttgtatgctt acgcttatgg agccagtcta aaaacggtta gacaacaatt 27480
cggtatcagc aaacgtcgct taaagaaagc gataaatgct ggaaactggt aaagaacaat 27540
gcggtgttgg tctagtcagg tctaggatgt ctccctgtca cggagaaggt cacgggttcg 27600
aatcccgtac atcgcgccaa attatagtgg tcttgctata gcgcatagag gttcgactcc 27660
tctatagaaa atactcgcgg gaatagtatt tgctcagggg ttagattccc caaaccacgc 27720
caaacaatgg gagttaccta gaagctggta atcgtcagaa gcgcaggcta aatcaacagc 27780
ctataatacc tgcgctgctc ccgcccaaac aatgtgccta agtacacggg ttgtgccgcg 27840
cgccttccaa gcgttgcaga gtggggttcg attcccccta ggcattccaa ttttggagtc 27900
tattatgatt gtgtttatca atgagagcca agaaatctca ctatattctg acctaggtca 27960
gtgtttcgat aacgaatttt attttcgttt tagtaagatt cttaacacag aaaccggaga 28020
actatacgtc tatgacgacg ccggtgctct tgattaccaa accgtaatag cagtcttcga 28080
cgatgagtac gaagacgaag atgaataaca atggtatgca ctgtcgcggg aaagtgctcg 28140
actgataatc gagaaaggct ggttcgactc cagttaccag taccaattta atgcacgcat 28200
agccgagggg tactacgaat gccctatccg taagaggagc tgaaaatggt ggttcgagtc 28260
catctgcgtg tgccaaataa tgcgttgcta gtagattcag aaataagcct tcgaggatac 28320
tagggtaaca acgcaccata taccggtgct tagctcagta gtgttagagc gctccgtttg 28380
ggacggaggg gtcgtgggtt cgagtcctac agtgccgacc aattttgaga cgttagttca 28440
gcaacaccca agatagctgt taactcaata atgcgggcag aaccgagctg tcggttgtaa 28500
agtcagaaca gtcgttgagc ttgtcgcagg aggacagcct taatcgaggt acgtaaaacc 28560
tcccaaatta gggggtgtga ttggatttct agaccagtcg ggttttatcc agcccctcca 28620
atttcttggg tgcgcctgtg gttcaggcta ctgtttacag tatcgaaaga gctttgaaga 28680
ggttcaattc ctcgccttag accaaacaat agcgggttag tttaattggt aaaaccttgg 28740
attttgattc caaagtcgca ggggttcgaa tcccccaccc gccgccaaag gagagacacc 28800
agagccttgc aacagttctg gtatgggatg gctagcaaga gcctacccac ggagtgccta 28860
gtatatgtac tctcggtcga agcccactgg taatgggaat atataaagct gccgacacct 28920
tagggttagg agcttcggaa gttcacttct ggattctagc aagtccgcta taaaccgtgc 28980
cgaaaggcag cgcacgggga atcacaaaag tgtacttgta cattttcggc tgtatctggt 29040
cgttagaata atctatcgaa aggtaggaaa gtcattaccc gcccagggtg gctccggtct 29100
tcggacggtt cacggataaa agtacgagac tcctagatat aatttacctc tagggcttcc 29160
cgaaagaaaa ggggagaaaa ggtcagaaaa ctagagcgag gttcatcggt aaggtatgcc 29220
ggtggataca tagggggata accaagaccc taatgacgac cgcaatcgtc agcctcgatg 29280
caactctgga agaagaacga aagtctaacg tataacacat tagtgcgagc agtcatgtaa 29340
gtgcggtgtg taccaaaaca aggttgggtt gaagaatcta aatcttgcac ggagtttagt 29400
agcgggaacg tgaacgacat gactgagtgg aatatccggt tatccaaaag gtaacgggct 29460
cctgtgggga cactcagttg ggaatgctta aagaagtaat tatgaatatt tggttttcat 29520
acaactaact tacaaaagca aaagagcccc acggtacatg ttgaaaggcg gttaattcct 29580
agccccctca aggaactagg agcatagaag gtcgcaagcc tgatatgttt tatcggaaaa 29640
agaatttagc gtcttagcgg atgcgaacgg gtcgcaagct cggaaggaat ggaacgatag 29700
agtaaacatg tatgacactc ccacagcatg gagtctaaat attgcaccgc tgttcggata 29760
ccagtgagaa ctggtggata agccctaaga catacgggta gtgaaagacc ggacatttaa 29820
ggctgtaatc tcaggccgaa tcgtttgagg acactttgag ctatgctttt tgatacggtt 29880
gccaagtatg gcctgaataa ggcaagttac gggtcgtcgg actcctagag tatagttcat 29940
caaagtgaaa cttataacaa cggagaaact aaatgcgtaa aatgccagtt aaagacgtct 30000
cacgagtaga gtctgaaatt gacaacgaaa tcgagttcat caaagaccga ttggggatgc 30060
aactacgagt gacgcaagga actcgtcagg atgcctacaa agctgcagaa gtcgctatca 30120
gtgcagcagt ggagaagatg gacaaactcc taatagccaa gttcgcttta cgcgacttga 30180
aggcggcctt taatatcgaa aacggtatta atgaacgtac cctcagaatt gcaatgctta 30240
ctgagcagaa gcagtttatg gaacaaaaca tgtctcgtgt gggtgacgtc tcaacgacta 30300
cctcctacca agacagcaaa gtaaggtacg tcccaggcgt atctaatgaa tctcaagaca 30360
aagtgcgagc cgatgttcgt aaccttactc gacagattca gcgtgtgaag gattcatgcg 30420
caggcatcaa cggaaacggt aaagtagagt taccagaaat ggttagcaac attctcgtag 30480
agtttggctt tattgatgcc taagtaagtt ttaaaagatt ggttagcgtt ggggttctac 30540
cgcttactat taaagaatga gtgtcgttca cgcagatgcg cttttatatt tattgtgtaa 30600
ttgttactct gttatggact tggccattct ctgtgagttg tgaatctgtt caattgtctt 30660
ggtaggcacg tcagcactaa ccggtctttt aattaagtgt atttttgacc tagccgctac 30720
agctccgtac taggtcaaaa atacatttta atgctcgggt ggtggaattg gcatacgcgc 30780
tggacttaga acccagattt tgagagttcg agtctctccc cgagtaccaa acttaagggc 30840
gaacaatcgc ccttttattt tctgctcgcg tggtcaaatt ggcataggca gtagacttaa 30900
aatctacggt atgtgggttc gaatccctcc gcgagtacca aattctagga gacctgttaa 30960
gagcctcact ggtctttaaa gtatcgaaag aggtctgcgc tacacgtggc atacgttaga 31020
ccgtaagggt tataaatatg tcggagcatg gaagcagcat gccactaaca aaggaagagt 31080
aagccgaaag attctgggta ccggcaacgg tcttgaaaac cgtcagtcac ttaacgggcg 31140
tgtgggttcg aatcccactt cttccgccaa acaagagcgt tcaggaacca tgccctgagg 31200
acgcgtattc gacctgatgc cggtcagctg cggtgagact ccgaagtgtg gaataattta 31260
ttaaaatctt caatagctaa acagcccaaa tttctgtata atatatttat agaatgaaga 31320
aagggcaaga acgatggatt ttaacgatga aaaactagag cgtgttggta agattgttag 31380
tgaaatgcga tacaacggag ctgttgttca ctttgatggc gaaaaccctg acaccgcagc 31440
ggtagatgtt ctaggacaca acactcgtcg ttttgtaaac gtcgagtacg tagatgaagc 31500
tatcggtgct ctaaccatgc gagctatgct gggtgatact tctaaagcgg atgctctgct 31560
ttacgaaatc ctaatttgtg ctggtatcct atctgaccct gacaaaggaa aagagtaatg 31620
actaagctag cagagttaga gaagaaagta gcttatttaa agaatgacca cgctagagaa 31680
agactaggca gcctaatcag taaaggtatg tttaatgaag ccttagcata tgtaatagga 31740
agcctagact acatagattg gtatgaagaa gagcagcgtc ttgagaaaat caagctacta 31800
ctactttaaa ataatttaaa gccttcaaag cattgctggc gatgcactgg atttgtaacc 31860
cagtgaattc ggttcgattc cggatgaggg caccaaacaa tgtgggaggg acgctgggcg 31920
tcggtcggat tccaaacccg tacctgctag gttcgattcc tagctcctat gccaattttg 31980
attcttttac tgtattgaga aagtgctatg gctcaccgga gccctctaac gacagatgaa 32040
ctcaccgctg catttgcatg tgaccaaacc aagctaccat gtagtagtta tgaccgcaaa 32100
taatcctaat taagaggtcg ggtatacaga actagagtcc cttattttat ggttgtaacc 32160
acaacccagt ccaccgatac tgattaggaa ggcggtaggg taatccccga atacagatgt 32220
attcgttagc gttttagtaa gtaggttgct ctagcgatag taggcaacat gtacactaga 32280
gcgttaacta atacaataca catcagtagg aaataattat ggatatacct gggtactcta 32340
agtataagtt taaccacagt acccttacag taactaattt acactcaggt aaggagctgg 32400
tagttggtaa ggatggaaga gtaaagataa aatcagactg tggtaagtgg cataaaaaga 32460
gtattagcca gttaaaaggc ctagctaacc cgattaattt agaggactgg gacctcgttc 32520
caggcacaga aggactatac attaataagt ctggtcaagt gctctccctt tctaccgcat 32580
ttcagggcgg aaagttgtta gagccttaca ttaataaaga gggttattta acagtagctg 32640
ctactatatt agggaagaga gcacctatcc cagttcactg tctgttagcg ttaacttacc 32700
tagatcccga ttatctggat aaagggctac tatgtaggca cctagatgat aataaactaa 32760
ataacaattt aaacaatctg gcagtaggta cttactcgga aaacctgtta gacgcctata 32820
agaacggcaa gagataaata aattaaaatc cctatagctt aataggtcaa agcgctcggc 32880
tcataaccgg aggaaggagc ggttcgaatc cgcctaggga taccaaatca cgcagcgata 32940
gctccagcgg tagagcactc ggttgaagcc cttggtgtcg atggttcgaa tccatctcgc 33000
tgcaccatat tatgccccac tagttagtgg tctatactcc ggagatggcg gttcgattcc 33060
gtacgtgggg ctgcaattta ggagaacgta atgtctaagt ttgtacaatg taactgcggg 33120
gagacgcact tctgtcactt aaagaactat gtaacctcaa caggtatcgt ggtagatgtg 33180
attgtctgtg atggctgtgg tagatactac gtagaaccag agaacgctat taacatgaac 33240
aacggcaagt ggaagtggaa gaaagatgaa taaagaccca gcatttcaag aatggctcga 33300
ccgtcaacct aagtgtgacg agtgtggtaa gccacagact acactacaca agcattatga 33360
gcattgttct aagtacgagc acatagttga cgaccattac gattctgcat gtatgcgtgg 33420
tacgtgtaag cactgcttcc ctaattacgt tgccaaggtt actatggaag ttaccgagca 33480
agaaaagtct ctaataaaag cacttagaga cccaggtagt tcaatggctg ctatatacca 33540
ccacataaag aaagtagcgg ctttctttaa gaaagtacat tagtttcaga acaacaggag 33600
taccgccaaa ccgtagggct tctgctgaaa gaccgtttaa ctgaaagcgc ttgcacggtc 33660
ggaggggtta aagtcctcag caagccaaca aattattctt tgagcactct gtacagctag 33720
tccctctctt ggttgagata ctagctctgt tggagtagtg acctgaggaa accaaaggct 33780
aggtttacac tgaacccaag tctgcgagga tagagtgctc aaaaaataaa acggaagata 33840
atccataact ggtgtgtggg actccctgct aaggagattg cttggaaacg ggtgcagttc 33900
gattctgctg tcttcctcca gattaggagc cagtaatgta ttatgtatta gttaataagc 33960
agggtcacta ccttgctaga cttcaaagaa gcgttaacac tttaacgacc actagacacc 34020
tacaagaggc gatgtggcta cttcataaag actatcgccc agcaggtttt gaggtcaaga 34080
cacttaaaat tacacttaaa tagaagatga accctgatgg tgaaggggta cgcctggaaa 34140
gcgttacgat tgtggtgaca cggtttcggt tcgattccga cttcttctgc caatttgatt 34200
tacaagaggt gaaatatgtt taaaggaatt cgtagatggt tccataagac tttcggtaca 34260
tgcacttggg gaaccccaat taaaaccttt gactttgtgc ctgtggaaca cgccaatatt 34320
cgatattgca cccatgaact gtgtaataaa acttgcacca aaacggcaga cctagatact 34380
attgccaaat acaaagaagc ccaaagaata gagcttgaaa agtctagggt aagtaaggca 34440
gcgtcagaaa aacagccaga gacagttgca gtagacaaag cggcacgtag agcaaagcgg 34500
gcactgaaac gaagtaaatc ctaggaggga ttatgattta ctgcaaagac actcaaggct 34560
acttacgcag ggtagcctat ggaaccttca gccaaaactg gagtcttata caaaacccta 34620
ctagagttaa agaaatgcta agacagactc taaacatcca cacgaacgga gcagtcttgg 34680
tacttgtcaa ataaccccca agggcgcgaa cgaaagttct cgccctattt ttatatccga 34740
ggtgattatg tcacaattta agaaacgtaa taaaaccttc ctatcagatt cccacgagga 34800
atttggtagt gtatcctggt atgttaagga aaggggtgat tggggctctg acgttcaaag 34860
cgagattcgc atcacagact gttacaaggt agtaacccta gacttctgcg cagaaagccc 34920
ttcagaggct aggaagcgcc tagaaaagtt ggatacttta atttctgagc tgcaagaggc 34980
tcgaaaagcc ctacaagaat gcgttgattt acgctggaac cgtaaataca tttaatcttt 35040
attagccttt tgtccgattt ttccgtataa tacttgtata aactgaaaag aagggataaa 35100
agatgagtaa ccacgtaatc ctaagcaaag aactgtacac taaggtaatg gagcagctaa 35160
accagttggc taactaccgt gacgattctg aagaagtatt gcaaggcatt ctgacagaag 35220
tagaagcggc aaagcaagcc agcgaaggcg tagtccctgt acataaagaa gacttatcta 35280
ctgtaatttc cctagcaggt tacgaagtag agtatgccga cgaagaagac cagaaaacat 35340
acgctaagtt agtagatttg actatagggg agtaggctat gcaagggtgt aaaaactgcc 35400
acgaactttt agacgttcta ttagaggagt gtgaccaatg tggtagcaca gacctggaac 35460
ctgtagttga gtgtgttcac tgctcttgct gggtttctga agaccacgta gacagggcgg 35520
gattatgtga ctctcaaggc aatgattgtg cctctgaccc tgacttagta gatgagtacg 35580
acggcctagc cgtcgatact atcttagctc tatctaaaca attattgcga taggagtgtg 35640
tatgccccta acagaaggaa aaacgcgtca agtacctaag ccacctaggc caccaggctt 35700
acggagtaga gggccaacta gggaacaagc agtatctatg gcctttactg taggtacagt 35760
agtaggtgta atcatgggca tagtatttat gttcgttgtc actagtatag cagctatgca 35820
gtaaattatt aaaatcttta atagcttaat agcctaaatt tccgtataat atctgtataa 35880
attaagaaaa ggaatcagac atgtcaaaaa cagtaattga tactgaaacc ctagagaaga 35940
ttctgactct tggtaaggta gcctctctat ttatgttggc ttccaaagat atggtagcac 36000
atcacggagc agagtccttg ctctcccaag gagagcaggc tatcaaggac agtatgggtc 36060
ttcgtatctt cactaaagaa gaagtaaccg cattagaaaa gcatttcgac ctcggggcat 36120
tcttcgagga ataacaaata atgggcgata cgctgaagac tcgaagcgcg ggattgcaaa 36180
ctccgtctta gtgggtggga gtcccacatt gccctccaaa taaggtgaga aaatgccaaa 36240
aaatagagca gaaaagaacg gtctaggggt atgggtgtgt aagcccgtaa aagaggaaga 36300
agactggttc tccaagcacg gtagttttat aaagggtgct atcttaggtg cctcagtaat 36360
gtatatacta accctatttt agaaaccact ctttggatag tacaatgaaa gagattattt 36420
taaaagccgg ctataccctt aaaatattgt cttgggagaa cgatgccgac ttctataata 36480
ccaaaactta tcacgtagag gatagggatt gggctattat tttaaagcat atctgtgaga 36540
acttactatg ctctcgatac tctagctcta gtgactacgg agtaggtaat actaacctat 36600
ctagtagcga cagtgctaac agagtagcag agtattttaa aacctatggt attccagaac 36660
acgttccaga agacgaactt cttgaatggc tacacgacct atcctacgat ttagtaggta 36720
gctcctacga aagtgattgt acacgagtat gtgagtcgtg taaagtcttg tacactccag 36780
tagatattga ggtagaaact ctatgagtaa gaatcgtggt gaagataaag gtatcggtgt 36840
atgggtacac gtacctaaac gagaagaaaa tgagggctgg ttctctaagc acgataactt 36900
cttcgcaggc ttcctagttg gagcatccct tatatttatt atggctctcc taatctttta 36960
ggagccacat ttaagtactc ttgaacgcta aggacgtgag acacccttag aatacggaaa 37020
tccggtatcc actacagggt ctcaacggcg gtggattgaa ggctcctata atagcggctg 37080
gagcgatgcc atgggaagta gctatcccta agagctgact gtgtagagtg tttaaatgtg 37140
gcgttggctg atgtgtaaag ggctccgggt tgtgattccg gaaaatgcgg gttagaaccc 37200
cgtacgccac cccaaattat cgcagtaccg gcttctgaac gctactcgac agaacatcgt 37260
cgactagata aggcaaaacg gcgtgaagtg actccacgta aatcgatttc taggcgtccg 37320
agtctatggt ggtaaattcc tgtagtcgac aatgggttcc accacaacca actaactaac 37380
taaccaaaga actaatcaag ttaatttata taggattaat atgttttcaa taaaactatt 37440
tctgccttct gagaaagata agtctatgtc tgagtcagca ggagtatccg cagtttacta 37500
cgctactaag ccggagtaca ctcaggatga ccaatttgta catcatgttt ttgagtgcga 37560
agatgtcaac gggggtaaat actactggcc actaacccaa ggtactaggg tttatgtaga 37620
gaactcagca ggtaaaacaa tagcccattt ctacggtggt ggccctatcg actagggcac 37680
atattaaaac cctccgaagt actagagggt tttcacatgt aaccgactaa ctatattatt 37740
ttataaggat tgatatgttt aaactattga ttaaattccc tctagtacga aaaatctacc 37800
gagctttcgt acctcttcaa acatctgaca gcctagctga gcagtttact ggcaagttca 37860
cgcgcgtggt tcaggagcaa aatgagattg ctaaagaata cacccgtaaa gaagtagagg 37920
cgaatgcccg ccgagtagca gcagagaaag aagccgctcg tgcagctaaa gccgcaaaga 37980
acttccaaga tatgctggag aaagaataaa ttctcagtaa gcccctttgt aatcaataag 38040
atgtcaatac ggcttctaat tatagagacc agacgacttc ttctagttac aaaaaatccc 38100
gtctacctca aaaggctaaa ggtagaccta aagaattact tagccataca aaatctacta 38160
gagcaaggag attagaatgc ctctatttaa gggtacggta ctaagcgaaa agttcggcgc 38220
taaaatcgaa ctactttccc cagttaagaa cggctgtgca gtcgttcgag tactggaagt 38280
tggtgagatg ctagaaggcg tcaacaagaa tcaagttgta aaagactttg cagttacttt 38340
tggcgttctg cgcttcactc ctgtaaagta acacctacgc ggggtgtccc cacatctccc 38400
ttctaaggag atagcgtata aaagtgggaa ggagctgagg ggttcgattc ctccaccctg 38460
ttccaatact aactaaccct gacttgttca gggtttttta ttatttgtca aaaagctata 38520
attacgatat aataccttgt aacaatagat aatgacttag aggaaataga taatgtatgt 38580
aatttcaggc gatgccggag ctggcaaaga taccgtaggt gcgatgttag cggaagaatt 38640
aaaggtaggt acttatgcct tggccaaccc tatcaaagcg ttactaaaag ctctgttcaa 38700
tattccagaa caccaaatgg aagaccgagc gtacaaagaa atggagatga cgtaccatgt 38760
agacgttact agcctagaag aagcaggaat gttatataac gaactagggc tagataagta 38820
tcaagagttc catgatgctt gggaagagtg gattgcgtta tttggtctag aagagagcgg 38880
agagtctatg actaccttcc gctccctacg tactttaatg cagctgctag gcacagagtg 38940
gggtcggtct aaatcggaca cgatttggtt agacctagcc cctgagagcc aagttatcac 39000
ggatgtacgc tttgacaacg aagcccagta ctttaaaaat aaaaactacc gagtaatcga 39060
agttgtactc cctgggttag agaaaatacc ctcatctggc catgcgtccg aagcgggaat 39120
ttgccctagc cttattgata ccgttctaat caacgacggc aatcttctag acttacgaac 39180
caaagctatt actatagctg ctaacggaga tggcccgtta ggtgtattat aacccaaagc 39240
ccgccaagtg cgggcttttt tgcgtgcaac gaaagccaca ctcatcattc gaggtatgtc 39300
aagtaataat tttaatctat agttgacacc atccccatat ataggtatac tgtcaaggta 39360
gggttacaaa tttaaagacc agtaactaac tggtatatgg gaattttgcc aatgaattgg 39420
aaaacagtag ttatcaatgc tgctatccca atactattaa ctttatcagt aaactgggtt 39480
ctagagcagg gcagtgccga caaagccttg gaactcaagt atagcgagtt ggcttctaaa 39540
gtgcaggtat tagaggacgc agctaaaggt acaaagatag atataaaaac ctatagctta 39600
gagataaatt ctcttagaga aaggatgcta aaggtggagg gtgacatcaa tcgcttaaag 39660
ctagatgtta atgcagcccc caacattcat agaatacgac aagatataga atctataaaa 39720
aaggatatag acgttttaac tgtcggaaga ggcaacagta ccgcgctact cagctccata 39780
aggctccagc taagtggaat tgagggtaag ttgtcaatta tagaacaaga ggtatttaaa 39840
tgattaaagc tattatacta gcgttagtat ttctatgtgc tcctgcctta ggtgagcaat 39900
gtagtgaact aacaaaggta gagcctaatc atctacctga atatcaagct taccaggaaa 39960
gtcttcaaga acaactcaac gcggggattc cagaagataa atttaattat tactgcatca 40020
ataatgtaga atatcagatt agtacagtgt ctgtactacc agggtaccag aaggagcagg 40080
aaatggtagt agagatattt gaacaactag aaagggcatc tgaccttact gatagaatac 40140
tagacgtagt acctattaac aagctagcca agagcgagga gtgattgctg gaaacttata 40200
gttgcctaac tgtctcaaaa ttgatataat atttatatta atcaacgaaa gaccagaaac 40260
ctaggagact aaatggaacc acaaaaacta accgtaacag gtgatattag caaaaaccgt 40320
gtaggtgctc agctaccttc tgaagcacct cacgtattca tttcttaccg accaaaccca 40380
ggaggagttt atcaggtaca cctagacgag ggaatttccc tagcatctaa gtaccgagac 40440
ctaaagcatc tactgcgaaa tgcctccgag tcggatacct ttgaaatcta ccttaacggg 40500
ccaggcggcc atctaagaac gtgtatcgaa ctggttcatg agatagctaa taccaaaggt 40560
agggttgttg gacacctggc aggagaagcc cagtcagccc acgctaatat ctttatggcg 40620
tgccacgaac atgtagtata tccgtatagt ttgctaatgg ttcatacttt ctctggcgga 40680
ttttacgcta agggcgagga cgtgactcgt gctgcaaaag cgtataacga gcttactcga 40740
acttgttata ctgacctata cacaggtttt ctgtctgatg aagaactgga agatgtactt 40800
cgcaataacc acgacagata ctacattggc caagagattc ttgaccgctt agcgggcacg 40860
tacgctcacc gcgaaaagtt agcacaagag gcggacgaac aggctcagga agctatgcaa 40920
gaggcattaa aaactctaga aaacgtgcaa tccagcccca atgacgtagc accacctgaa 40980
cagcctgagt cccctgacga agaacaggaa aatggcgaat aaatactcaa aagcaagtag 41040
agaacgacta gatacttgtc accctctcct aattaaactt atggagagaa tccttcctag 41100
atgggaccac accattgtat gtggccaccg ttccaaggaa gaacagaacc gactgtatgc 41160
tgaaggaaaa tcacagctac agtatcctag gtcaaaacac aatgcctatc ctagcattgc 41220
cgttgacata caaccttacc cattcaaaga cgaaagagac ctatattact ttattggata 41280
cgtgaaaggc gtagctgatg agatgggaat caagttaaga atgggagccg actggaacga 41340
caacaaaaca gttacagaca actggattga tgctttccac atcgagctag tactttaatc 41400
gaaccctgca ttaacgtgca gggttttctc gtatgaggag cctatgaaga tatacctatt 41460
ttcatccaac gggtgttacc cctgtgaaaa agcacaggac gtggtatcaa aaatactaaa 41520
agaaaatccg ttaatagata ttataaaggt tactagacca gacccgctat ttgcaaggta 41580
tggtgtggtt atggttccca ccttacttat cgaccccggc gataagccag gtgcggcctt 41640
ggtgggcgtg agcaacatta caaaagtcga agtcgaggga ttactacgag atttcggatt 41700
tcttcaatag ccaaaacatg taaatttcgc tataatattt atataaattg aatgagagga 41760
aatattaatg gaaatgttga aaccagatat gagcaaagca gaacttcagc acgtactagc 41820
tatcctgcta ggcaacggta tttctgctga agaactgaag ccccttactg tgccaatcct 41880
taacaagatg cttaccgctc tagagactaa tgctaaagca tttaacgcac tagaagataa 41940
gtatcgagag ctgcttcaag ccagtactta caagcctatt gctcctaaaa cacatcgtaa 42000
ccgtggggcg gagaaccgcg ccatggctaa agaacttcgc aagctaaaga gtaacaaata 42060
aatgcaactg tattacttat tcttaatagt gctagcacta tacgaaaaca tcgacacaat 42120
caattactta gacgcgaggt acggaagtga aattctctga taaactaatc gaactggtag 42180
acgaatacga cctagagggt cctcacctgc cttacggctt cgctgtagag catattaacg 42240
agaaaggcca gatggacttc gcgcatttta tccgtaaata taaagtatac ggctctgtaa 42300
aagatttctg ctcagcagta gaaactgtat ttggtacgtt acagtttacc tatgacgtac 42360
ctacagatac agtaaccaag gaagagttcc tagagattta cgagtttggt aagtttcctc 42420
gtgtaatgga ctggattcct tatgtagctt ctaagaaagg caagctaatc ttgcgtgaag 42480
atttatttgc ctgggtactt agtacttctg caccttccaa agctaactac agcatgggag 42540
atattaacaa tgtcgagtaa agaccccgta aatatcgaca caatttgccg tattctagaa 42600
gagcttcatc ctagccacta cgactcggtt attttgtcct gtaggtcagg tgctccacac 42660
tctctactac ctgagattga ttttagtgat agagagactt cagtttacgt agagtccttc 42720
ttgacaataa agaaaacaat taattgccaa tcaggcagga tttccgtata atattcatat 42780
taaataagga aacggaaaat gaattataca tcactagacc tacaaatggg gccacagcag 42840
ggaggtaagc tagatgcgct tattagaacc tctaccaacc tactacaggc taagcagccc 42900
ccaaaggttc ctttaatatc tgtgtctgaa agacacctac taaacgccta cacacaaaca 42960
ctaagaatag aaggtaacgt aagttcttgg gcggaatatg ttgctcaagc tcctgaacgt 43020
ttaggctcca aatttccaga acgagttagg ttctgggcta gcaagaaaag taatatgcca 43080
gcgtgtcctt tccttctagg acaggaggta gttttaccac tttactttga ctctatgaat 43140
accatgactg tgttcagcgt gtcaactgta agctacggag agatacacca aggcgtaagt 43200
tttgataatc ttatacccct attcgatagg ctattctaca ctagagtcct taataaggta 43260
gaggcttctg cagtagaaag aaataacatt agaagagcag tggtcacact gatggactca 43320
accagtgatt gggacaaagt tcgtaaagac gtaatccgta ctataaccct agaagcggag 43380
cggggagtgg catcggcgga aacggagaac atctgggaca ctcctgatat agtagtaaac 43440
ggcaacactc acactttcaa aaatgtaaca gttaaagata aacaggacaa taaaatgagc 43500
aagacaaaac gaatcgcaga gaacatggta gaaagcaaca aagcagcggc aaaagatatt 43560
gcatttttga acgcaggtcg tacagccaat atggttatca aagctgcaat gactccccta 43620
ctaaaagcgg tacttaaacc aactttcatg cagcgagcta tgatgaagct aacaggcact 43680
actgacccag tcgagcgctt catggaatct ccagtgtctg acctactagc tgcggaaggc 43740
tttaaactag ccttagagct taaaggcgtt aaagacgaaa aactacaaga aacagcagat 43800
aaagctatcg tttacgcagg catgaaggtc acagagaaca ttcctctaga agacctattg 43860
gatgacgcta tcgaaaaagt agcggaagcg gtctctggca gcaatatgct agataaattg 43920
gcagagaaaa ctcgtaggta ctaagtatgg gagcattact atatcttatc gtgtgtgcct 43980
tggtaggcta tgcagctgcg aactaccgta ggagccccat aggctggttc ctattctcag 44040
cggtctttag tcctatagta gggatgttac tcttactatt agtgggtagg aaatgaaccc 44100
tttggagcta acacaagcac aacaaaaact tcacgcagta gatagttggg ctgattggat 44160
ggctaattgg attaccatcc aattagaccc tgtaagcaac aaaccactac actactgctt 44220
tcacccagat aactactgtt ttctggtaac atcagaccct gaagaggcat gtaatggatt 44280
ttcaaaatat ccgtacgacg ggtagtgtat acgaagctat ccaaggagct attgagcagg 44340
ctcgctacac aaaggataaa gaagctctat cagtattcca gtacctagtc ggagaagtaa 44400
gccgacaacg tacagtgaat acttcggaca gcgcagttgt ccctcttgta cgaaaagtac 44460
agaaacgact tgtagagtcg gttgagcaag gagccactcc tgaaattcaa ttgacttatc 44520
taaatagctt tatcgacgcg tggctaccta agcaagctac cgcaaaagag ctagaggcca 44580
tggtgaataa agccattgag catctaggtg acaaagcgaa catcggttca gtaatgaagc 44640
tgctaaaaca aactaacgta ccatttgatg ctcgattcgc atctatccta attaaagaaa 44700
aactagcata aggaatacca cattgattaa acgtacacta gcaaaaatcg ccgtagtcgc 44760
tctaatggct acatcactaa caggttgttt tcgtggagag cgagtagaga ttcctactgc 44820
gttcgtgggt aaggttcaga ctgcggcagg cttcgagaag ggaaaagcgc gtaatccctc 44880
taagttccga cttagttact gtggcccgac cgaggcgtgt gagcgtctag taatcctaga 44940
tgtgtctgat aatcaataca ccgaagactt tcttacgtac atgccggttg accagcttaa 45000
tatgggctac cgtattacaa tgacactggc tatcgaccca gaggagcagg atggtctatt 45060
ccaaaacgtc ccattccgag aagtttctgg ctcccttggc gttatcgagc aggcacgagt 45120
gtataagaca tacgcacagc ctatcctaca aacaaaaatc cctgctgtag tctcgcaact 45180
cagcatcggt aaagtatctt ctaacctaga tgcggttaac gccgtacttc taaaggaagt 45240
aggtacaatt atcaagtcag agacgccatt ccgactgaag catatcggta tggttaatcc 45300
taagtaccct gacatcatta cacgagctaa agagctagca aaagagcgtg aagctgccct 45360
aggccaagtt aaggctaaga aagagctaga gctagcggag atggctgctg agaaggagct 45420
agaagcggag cgccgagcag tacagcttat gaaagcaaaa actgactcta tgattgcaga 45480
tgcagcatct gaccgttacg tagagcttaa acgcctatca gtgctggagc gactagcaga 45540
ttctaacaat aaagcgttta ttcctgtgga aatgttcgac caactaatga ttaaaggtga 45600
ataatcttgg agttactagt taacatccta ctagtaacga agcccgtatt agggcttcta 45660
ctactgctag tactagttct ttgtggttgt aaatacttta aggtagagtt accttacgca 45720
aagattctta agggttcggg tgtagtggct ttactggcat caattgccat ggtagccttc 45780
tccccgataa ctcaacctac tacgcaagtt tacgataata ctagagactt gcaggctatg 45840
gaagccaaga tagaccgaat ggaagcaccg tccttagata gtttcacctc taaggaagcc 45900
acactaactc tagaaagtat tcagaacgaa cactctaacg cttacgacga ggccttagag 45960
gaaatagaga agttcgaaat taactaaccc aatccctgac agcaatgtca gggatttttt 46020
cgtttgctaa actctcgaaa tttcgatata atagttatat aaattagaga ggagactatc 46080
ttatgcgcca taacgaagaa tggattgagc ttgtgttcgt tgacggagaa gtagaagtac 46140
acgagctagc agtcgatgct tgggagcaat ttgagcaaaa ccgtagtgac gtagcagaaa 46200
tttggtggat taagaaagaa ggcgatgaac ttgaaatcac agcagaacaa cttgagcttt 46260
ggtcaacgta tgaagaaatc aatgatgagt acagtggtga tgatatgcgc cttgactacc 46320
ttccagacgt tggcttcgga atctaggttt tccaaagtac ccaaagctaa tactacagat 46380
tgccacacgg taacaactag acaaggacga gataataatg ggtatacttg ggtaggggat 46440
ttgtacaatg atagatacac ccttatcgta gattaccgaa accctgtcga tttaaaatac 46500
aataaaactt atttactaca caacaaacaa ggttctccta tctttacgct agcagctaag 46560
cggctcaggc ataagatagc gataacctac tgccgatggg acgcaagcat atgaaaacat 46620
ttgaacatat agaccttaat gaccgagagc gtgtattctt cgtaggcgac atccacggag 46680
attctaagct gttcgaacat gcggtcaatt ttctaggctt atctgagaag gattacctag 46740
tatgtacagg ggacttaatc gaccgtggag aagctagcgt agacgttcta actagagtgt 46800
tgatgtccac aaatatgacc tctgtgctgg gcaaccatga ccacttcatg gttaaagcga 46860
tgaatgaccg aaaaatgtca aacttcgctt tatggtacca aaatggcgga tactgggcag 46920
agcagcatga cagagccttc gttcaaggat tagccaacgc agtcgaagaa caattccctc 46980
tagcactaat cgtatggtat aaggatacta aagtactagt tacgcacgct gaagtcccta 47040
ccggagactt aaaggtttta gaaagaaaac tgactccaat cactggctct gtaaaaggcg 47100
atgaagacct agcccgactg gttgctcagc agaagctgcg tgaagaaatc atgtggggac 47160
gtaatagaat ctacgaaggc gagcagtttg tgtctggagt ggacttgacg attcatggtc 47220
acacagtaac acagtgcgtc agagaagacg aagaaccttt acctttacaa ctaggaaatc 47280
aacactggat tgacacaggg gcttgcttca ccgggggtag gcttactata gctgagcttg 47340
tagacggaga gttgcagtac actcaatttt ggcttgattc agacaacgaa ctttgtatcg 47400
tataataagg agtcaatttg aaaattaacc tgactattga gacggataac atatccggat 47460
tagtaatctt agatatggac tctgcggaat ggggttctag accaatccgc agagaagact 47520
gggaggctgt agaggctatc aaagctgacg gtgaatactt caagattcct gaaggcacag 47580
aggagttctc tgcaatcttc acagacgaaa ataaaatttc ggaatacgaa gctctaggtc 47640
tggaacgtat gatgaagaag tacatttcag gtgatactaa agcaacagta actatgacgg 47700
ggagaaagta aatgttccgt aacctatacc gtaaatttct aaagtggctt gattaccatt 47760
ttgggggctt ttgtccagct aagactgaag ccgaaaacga acaagaagat aagatgttct 47820
cccgtaaggc agaaatctta aaggagctga aaaagaacga tggcaaataa gcatctacta 47880
actggaaacc tcccactaga ggtacgaaaa ctagtaatta aagacccgtc cctaatagag 47940
ggacagcacc gagaactact aaaaagccta ctagggttcc gaaccaattc agagtacgca 48000
ggctggcttg caacactacc taaaccaaag aaagaggaaa aagcggatga ttgatattgc 48060
ggtggcacgg tgtaaacatt gtggctcagt cattactagt cgcgctagcc atgactttaa 48120
ctcttgtcag tgtggacagc tgtccgtaga tgggggggtc tttgtagaag aaaacaacac 48180
acttagattc actcgtgtta tcggctcttt tcagcgtgaa gacttatcag taattaaaga 48240
tttccccgta gaataccagg acttgtacga tgactggaat aagggaatca acgagtacaa 48300
agatttacgc aaatacgctt agggtagcac gtaacactag gacttataca aacaatagcc 48360
ggtgaggcgc aggaatttta acttatgcga gaaaaagtca acatatatga ttttacgaca 48420
gatgtgttag agcaagagct gtcaaacacc tctgttgtgt acttaataaa ccaccaacct 48480
aagatgggtt gctgcgcccg tgcccgagaa agggagaaag gactctatat cctagaattt 48540
agtcaagtgg aaagcacgaa aaccgctata attcacgaag tggctcacgt actactgtgg 48600
gcactggaaa acaagtacaa ccatggtcat ggagccgact acatgagaat gtacgaatac 48660
ttggagaaca aatatctatg ataactatta aagcaggtgg ctcggagata gacgtaatta 48720
aagacctgac tacagaagta gagcagagcc ttttaccttt cggtgtaacc cgaattactg 48780
ctagaaatcc tgccttttcc acctttatgg tggagtatgg caagctattg gttgataaag 48840
ttgcttctgg cgagtttgac gccctagcgc cagaagtagc ggcgtatcac tatgtaaagt 48900
actgcctagc tgcgggaata accgttgagg tgactcgtgg ataggtggat taagtggaga 48960
gataagagct acttgatagc tcaaatgttc gatgcttggc gacctatccc taggttgatg 49020
tgcgggatgt acggatacct tatttaccaa atcactatgt ggtttatagg gcttccagac 49080
cctacaggag ctcaagcagc gtttgtatct actatggtag gagcagccgc agcatttttt 49140
ggtttctatg tcaattctaa ggctagaaag cctgacgata aagagtaaag gtctttcgag 49200
gtgaatttag aaagttttca ttcgcctcgt cgtcgatttc cttatataat ataaatataa 49260
aatcgagaga caaaaatttt tagtttacta ggagaggata tgcctatcac aaagactcat 49320
ggtttcatta tcagccgact tcaaggcttc cacaaaggtc ataggagctt aatcctcaat 49380
gccctaaagt atagcagcac gctaacagtg tttataggct ctagtaacaa agctggcagc 49440
ctaaggaacc cgttcacgta taaagaacgt gtgaaaatgg cgaaaatggg tctgccagca 49500
ggcattccgg taacttttga gctgctacct gattttgact acaacgacga cctatgggaa 49560
gagtacctac acaacaccat tggtatgctc actaaagaag gccatacccc caccatcttt 49620
acctctcaaa aaggcgaaga tgacctccta agaaaagaat gggctagaga catcgaagtt 49680
atctcgttcc ctgtacacaa acaaattagc gctacggatg ttcgcaaagc tttactagcc 49740
tcagacatgg attacttgaa cgagaatgtt cctaacccgt ctattctagt tcctcttatg 49800
aaagagtgct ctaagcctat gtacgaaaag catatggctg tactgcgtaa ccagtatgtt 49860
tggaaagact ctccgtatcc ggttatctct agctgtacag acgcagttat cctagacagg 49920
gagcgccatg tactcctagt gagacgaggt aagcacccag gcaaaggtct gtgggcacta 49980
cctggagggc atttagagcc gaacctaact gaagctcaga atgtatacaa agaagtccta 50040
gaggagacgg gactagaaat taacgataca cactacgtta aggacgaccg attcgacgct 50100
gtagaccgct ctgaggcagg tcgtattgtc actacagctt tcgcgttcca agttaacagt 50160
ctcttaccag aagttactgg tggagacgat gcagcagaag ctcgatggtt taccatcgca 50220
gagctgaggc atctaaaaat gtacgatgac cattatggca tcataatctc tatgattaga 50280
gaatttgaag tggactaata aggctttacc ttatcccaca aaactaacca gaggtttcac 50340
ctcgcagcag ggttttaccc taaaggaaac atattatgtt tgtaaactca aatatctgtt 50400
tggcaactga ctcttacaaa gtctctcact ggctacaatt cccacaagga atgaaccgtt 50460
ctcactacta cattgaatct cgtggcggag tctatgatga agtcatggtg gcgggggtaa 50520
aatacctgac atcaattctt aaacgtggtg taactaagaa gcaagtcttg aaagcgcaac 50580
gctactggaa ccttcatttt ggacaagacc tgttcaatat tgatggctgg atgcgtatcg 50640
tagacgaact tgacggaaag cttcctgttc gaattagagc agctaaagaa ggcgatgtta 50700
ttccagtgaa aaacgtactt ttcactattg agtccacggt tgacggattt ggctggctcc 50760
ctggatacct agagactatg ttactacgtg gtctatggta cccaacgact acagctacta 50820
ttagttttaa agctaagcgc tttatcaaag aataccttga catgtctact gacctagtag 50880
gggatgccta cgatatggtt ctgaagactc gcctgcacga ctttggagca cgtggcgtat 50940
cttctgcgga gtctgccggt atcggtggga tgggtcatct gtacaacttc gtgggtactg 51000
ataccattga agcgactcta ctagtagagt tcttgtacgg tgtagagatg tcaggtatct 51060
cgattccagc tcgtgaacac tcaactacta cctgttactt acgtgatggg gaagatgagg 51120
cgttcatgaa ctctatcgag aacttcgggg tcggtacctt cgctatcgta attgactctt 51180
actctacgga cgacgctttg cgtcgtatcc tagaccctaa cggagcactt cacaaagctc 51240
tacgtgagaa aggcggcata tgtgtacttc gtcctgactc tggagaacca attgacatgg 51300
ttatgaagtg catgaatgcc gtgtggaaca ctgtaggcgg agaagtcaac tctaaaggat 51360
acaaagtact agaccctcag taccgagtaa tccaaggaga tggcgtggat ggccaagcta 51420
tcgaacgcat ccttaactgg atggtaggcg ttaagaagtt ctctgcggag aacctggcct 51480
tcggtatggg tggtggcctt ctacagcact gtgaccgaga tacacagaaa ttcgctatga 51540
agtgctctgc tatggaagta gacggagtat ggcgtgatgt gtttaaggca cctgaaactg 51600
accctagcaa aaagtctaaa gccggtcgtc tagatttagt ctaccgtggc ggtaagtacg 51660
aaacagtggt actaggagat acagacgtac gtcaccctag ctccgcttta cgtacagtgt 51720
ttgaagatgg tcaggtgttt gaagaaaact tccgtacctt ccaagacatc cgcatggcat 51780
cagattctta cgcgtaacat aacaggcggc gaaagccgcc taacccctgg agatagctaa 51840
tgttcttaaa taagagtatt ataacgatac tagctgcaac gagcctagtc gcatgtagtc 51900
cggaaaagaa aattgtatac gtggacaagg atacagggaa ggagctatct acgggccaag 51960
ctcctatagc caagaaggtg gacgtatacc tgaacctacc agtagagaac acggatacct 52020
ggtcagggaa gtcttatcac ctacatcgcc ggtgtataga tgggtaccag tactacttcg 52080
agtacaaagg ccacggttcc gcagccttcc cagttctgta taacgaaata aacggggcac 52140
acataagaac ttgtgaccct aaaaacttca aagttaaaaa aggtaaatag gaggcttcgg 52200
cctccttttt tcgtttcttc agttgtcaaa tccctcaaat cggcgtataa tatatttata 52260
aactgaaatg aagaaaagtc tattactaga cgccaacgga ggtttcacat atgactggta 52320
atacgctaaa agtatctcca atgctggaaa gcaacgaacg aatcacctac tttgtatacg 52380
tagtagcgga aggctatgtc agtgagtttc gcacaccaca gctaagctat cagctagctg 52440
gcaccaccaa cctagaacaa gctatctatg acactttccg atacgcccat tttcatgggc 52500
tatcggaggc ttgtgtgacc acagcttttc tggatgcggt aggtgaccta ttagatgtag 52560
agtacctaca atcctcttta ctgtcgtacc aggacacatt tggagacctg tacgagttta 52620
agaccacagg agtacgtaat gaagaaagaa aaactagctg agctagttgc cagaggagca 52680
cataaaggac agaaatacgg cggctacgac tacggttacc atctagaaat ggttgtaggt 52740
atggctcgct ttctttaccc agagtccccc aaactaggcc taatccttca aggctgctgg 52800
cttcacgatg tggttgagga tacagacctt agcctagatg acctgagaga catgggcttc 52860
caccatgaag taattaaggc ggtcagttta gtcaccaaag atgaaaaata cagcaaagat 52920
aagcacttcc tgtacctaac ctcgatagcg caagacagct tagcctttaa ggtgaaagtt 52980
gctgacacta tggccaacct acagcaaaac gttagggaag gtaacttcag cggtgttagt 53040
aagtacacac tacaacttaa agacttacac ggagccaaga gccagaggag cacaaatgca 53100
taacccaaga cccccaagac caaacgtacc aagaacccaa aaacccatta tcgtagagct 53160
gcgtagagta ctgaaagagc ctcttagaac caagaaggta actcaatcac tgtttcctat 53220
ctccagtaga aaacataaga aagaagaggc cttcttcctt atcggacgac tagtaacagt 53280
aactagaaag tggttcggtc tatctagtaa aacgaaaact gagttcgttt acgtagtatg 53340
cctcggtagt tcaatggttg ggtacaccgg aaccaaagaa gcggcaaaat ccgtagtacg 53400
aaccttaaat aaagagatgt cttaatgaaa gaactaaatc tagtacaact acatgactta 53460
gtctcccaag gcctagttcg tactaaagaa tacccagccc ttgggctaac tatacataag 53520
tattctcgca aagtgtttta cgacgctctg tggggtacag ggccaatgct agaggaatct 53580
cgcggactag tattagactc tgaaggtcgt gttatcatgc gccctttccg aaaagtctat 53640
aactacctag aaagagatac ctgtaagtat gtagacccaa acatgaaagt acgtgtcgtt 53700
gaaaaagtca atggcttcat ggcccaagca gttaaattcg caggtcaaac cctaataggt 53760
actacaggca cattagactc tgattactcc aaactagcta ggaaggttat cgagtccgct 53820
actcctatag aggagcttta cggtataact gataacaagt tccgtgggtt tgaaaactat 53880
actaccttgt ttgagatatg tgacccatct gacccacata ttataccaga agaagcagga 53940
gcttacctaa taggtattcg ttgcaatcaa acaggtgagc tactggacga aatgaaacta 54000
gatttgtacg cacatatctg ggggttcaaa cgccctaagc acttcgtaac taccttcgaa 54060
gacgcggtgg ctatgcgaaa aacctgcaaa catgaaggat acatggttca gtccttgaac 54120
ggagaagtac tgtgtaagat gaagtctcac tactacttgg ctaagaaggc ctttatgcgt 54180
atgggtaaac aacgatgcgc cttgatgtat aagaacccta ctaagtttaa agagcagata 54240
gacgaggagt tctatggggt agtagactat ataaccaaag tgtacaccga agaagagtgg 54300
aacggacgta ctccactagg tagggaagat gttttctgct ccttctttgc tgaaacagca 54360
gataataact tttaggagta tttatgacta ctagaaaagc gccagaagaa gttaagcaat 54420
acaggtctag agatggtata cttttcgata ccatgactaa tgcagggtat cgtgaccagc 54480
tactagacct acgcgaagca tgttccaatg caatcaaaaa actcactgag tatgtacgta 54540
aatgcgaaca ccctgagtat tctgaaacgg gaactcaatt ggctttgcgt gagaccgacg 54600
tgcaagatga gtggggcact tatatgtctg accaagtaga gcaagtcaca tataaatgca 54660
agtgctgtgg ggctataggt caccgatggg tggatagcga ccaaatcgta ttctaccgcc 54720
cagttaaccc aatgtgggat gcgtaaatga gtaaagtagc ttgcctagtt ggttctagga 54780
atattacaaa tcaggacaga gagcgcatac gtactgtagg gatactactc tatgggctgc 54840
ggtacatagg ccgttctggt aacgctgaag gtgtagacca agagtgggat aaatacctat 54900
tcgttcaaca catactaccg tgggatgggt ttaacggagc ccataacggt gactgtgact 54960
accaatatct agccctaaac tttgctcctg aggagcttag agctagggca gaggctatag 55020
ccatagagca tcatccttac ggagatacct taagggaggg cccactaaag ctccatacac 55080
ggaatgtttt tcaaccccta gggttggggc tgtctccgga aacctatgca gaactaacaa 55140
tatattgcgc cccagaatcc ccttatgggg tagttaaggg tggcacggcg acagccgtga 55200
gcatctctag gacttacggt attcctacct ataaccttag agacgacgaa cagtaccaag 55260
atttgctcaa taaattaaga gcagagctgg aagaaatgta aataattgct cgcttcggcg 55320
ggcatttttc gttataatat atgtataaaa tgaattgaga gaagattaga tgggtacgaa 55380
gcacgactta ttttatacat attcagatat gttagcctca gaaaaaagaa atctgcgtgc 55440
gtatgtacac atctctaata aagagagggc acaagattta gctaggcgta gacgcttcga 55500
cactagcact agctctagag ctcgcttggt gattgcgaca aagctattaa ttgctagata 55560
tgttatgcgt atgaaagaat tcgaccagac tatagactac tttaaataac caactggagc 55620
caatatgaaa aactacttat tcttttacaa actaggtgga gctggtgttg gttgtgatgc 55680
aacaggtatc tgctactttg aagtcgaaga ccctgaaaaa gttatggaga gcagcgaagt 55740
caacgagacc ttatgggaaa tggcttgtga caacgctgag tctttcggta tttaccctac 55800
tcctgactac gaggtcgagg aggacgagga agacaattac tctgaagata tctacggtca 55860
cgctgttccc ttcgacccag agaaacatgg tggtaaattg tctgggggtg gccactggaa 55920
aactcacatc gacgagccag gtactcctgt ctttgaagac ggctactggg ttctagacac 55980
tagcaaagcc gcctgtgaga aaatggtaaa agacctagag cttaaatctg tagacgagtg 56040
tcagtaccgt cttgaaaaga aaatggagcg tgtgctagct gaggctgacc gtatcgaacg 56100
tgagcgtgta tatcttgaga aagagattga cgctcttaaa cttaaagcac gtaagcttgt 56160
agctgctggt gctgagtatt ttgaagaaga ctaggagtgt ttagtgaaca tatttgtact 56220
tgacctagac cctaaagtag cggcaatgta ccactgcaac aaacatgtta tctcacagat 56280
gaaagagtca gtacagatgt tatgctctgc cttgctgatg aacgataacc cagtacctct 56340
aaataaatca ggtaagcctt acgagctggc ttacccaaac catccgtgta ctagatgggt 56400
agcggacggt attggcaact accaatggct gtgggacttg tccatggctc tggtcgaaga 56460
ggctgaaatg cggtttggtg gagagtatca tctagggact atactgcgtg atggttcttt 56520
gccgcgtgaa cctgttaagt ggttacgccc aaagcactct ccagtgcctc accgcactcc 56580
ttttgcaaat gctaccgcag actggctaaa gtcttccgat atgtgggata acccctctac 56640
gtttaaccag ctaaacgcga tagacatcta ccgactatat tacatgatgg acaagtgcca 56700
catgaccgcg gtggacatga aagaatccaa tatacattgg aaatcagcac acaccctagg 56760
acaggagttc actgacctag atatatggac taacagagga gcaccggcct ttatgagcga 56820
caggttttat gcacaacaat gcgaatattt cggtatcaag ccagcccagt taatccacat 56880
gggtagatac ccagaccatc ctgactctat caaactaaaa gaaaagatgg agcgaaagat 56940
gggcaagaaa gcttcaggta ccgtaggtac taagtctaag agagtcacca aggcagatgt 57000
actagtggac atccgtgagc agattggctc acaggcttgt gacggcttct tgaagcttac 57060
actaaccgac atgcagaagg tgaagctagg tttagtagac ttagaaatgc cagagaatat 57120
ccctatgccg gtagggagac taaaggcacc ttttgtcgat gcgctagcat cgttgatacc 57180
ttgtgacgct gacttctcaa aaatgacagt agcagagcta agagaaatct taacacactt 57240
tggagcagaa taatgagtaa agaagtatac gtagtactaa gcaacgagca gccctggata 57300
cctctaatgc ctctagaggg taaggtgatt caagctacca tcacggatta ctacctaagt 57360
gttacttgtc caggtggaga actctacaac atcgaggtgg acccggattg gaagttccaa 57420
ggtggtgatg cccagtttag tatcgtggat aaagatgtat tcgcagagca ggattactac 57480
actctggcta atatcattaa agagtgctcc acccctataa tggtcaatgg gcgaaaagta 57540
acaggggctc gagtaattga cggggaaatc catctggagg ttaaatgacg tacccaatac 57600
tattcctaga cttcgacgga gtcataaact cagacgaggg agagatagag tggttgaaag 57660
gtgatttctt tggtttcttc cagccacacc tagtagatag agtgaacaag ataacccaag 57720
ccgtacctaa ctgtaagtta gtaataagct caacatggcg gttagatact gatatagacg 57780
agcttgcaga cgtatgtctg cgtctcggct tgaccgcaga agttataggc aaaacgtgct 57840
cttttggtaa gtactctgtt agaggcaacg aaatctatgc ataccttaaa gacaatgcgg 57900
aagagttagg acataactat agggcttacg tcatcctaga tgatgactgt gatatgctct 57960
acccacaacg ccataatttt gttcgtgtaa acggaatgaa agggattcaa gagtccgacg 58020
tcgataaggc tatagccata tttctagaga atctttgatt gcaaagattc tcttttttcg 58080
ttataatact tgtataaaat gaattgaggg aatctttatg gaaaagaact actatgtaca 58140
ctacgttagt aatagcctac gtcgttttgg tgtccacgat gaggacgaaa ctagtccaga 58200
atactggaga gatgccgagg tagtcggccc atttacagaa gacgcaatgc gaacctttga 58260
ccgggaagcg tgtcaagacg tggccagtgc tagtggagag tctgagtggg aagtatgccc 58320
tcactgtgat ggtgaaggta ctacggaaga cttcgaaagc ggggagcctg taagttgtgg 58380
agactgtgga ggccacggag gttgggagga agactggaac gacttcgtaa acggtaatac 58440
agagtctatc tatgaagtgt acaacccaga actacattac atggcacgtt ccaagtttcc 58500
ggagcatcta gcttggaaga aaaaggataa gatgcagaag ctggcaaaca ccatggaagt 58560
tctagaacgc gagaaagcca acgcactgaa agaggttcgt aggcagacga agatggccgc 58620
gtccgctgat atgaagtgga aggcgttggc agaagaactg gctaaaatgg agcgtgagta 58680
gtgacatacg aagagtacat agataagata gaggaaattg tgtcccctga gttctctact 58740
atctcccaaa ttgaccatga cggtggctgg gttactgtag atggtatacc taattactgg 58800
agcacttacg tagttagcga actagaggcc aaggggctaa ccttccacgg caccgcacct 58860
gtaggggaga atactaccta ctactttaag cttaatctgg agttctactt aaaatgaaaa 58920
cactatacct aattcgtggt gtatccggct cgggcaaaac tactatggcc aagaccctag 58980
aggctgccct tcctgactct gtagcttatg ctgctgacga cttcttcaat gatgctcaag 59040
gcaattacaa ttgggagcgc aaacagctag gaagggcaca taattggtgt gtttctcaag 59100
tggctcgtgc tttggcagat actcatgtag agaacgtcat tgttcataac actttctcta 59160
ccgataaaga gatgcgccca tatattgaga tggcggaaga cttcggagca cgaattgtat 59220
ctttagcggt ggaaaaccgt catggtaact gctcagtgca taacgtgcct gaagcaacac 59280
tagaacgtca gcgacgttgt ttacgggaca gtctgaaact ataggagact gtatggagta 59340
taggagtaac gggacgtact ttaagaagta cgtcctagag cagatgttag atgaaatagc 59400
cttcagcttt ccggacgtgt tccacgccat actagtaagt gacccaatac actttaagta 59460
cactgctagg gtatgttgtc atatgagtga cgaccccata gaagtcactg aaaatatttg 59520
ggattacgac aaacttctac tcggtgtaga ggaaaaattg ataaatttat attgccaaga 59580
ttcgtgaatt tcgatataat atttatataa attgagatgg agaaagttat gttagttaaa 59640
atcaccaaag atgatgtagt ggctggcctt caggccacac tagaaggaac atgtacctac 59700
tctagctact gtagtacggc agtagaggat acctgctata acttcctagc atcttacgtg 59760
gctgaccact taggtggtcg aacgcctatg tataaatatt ccgacgaatt agagaagcta 59820
tccaaggctc tagcccacta cctagatacc gacgatgtgt gggacaaagc ctatgattta 59880
tgggcgggtt ctggcttttc gctatgcaaa gcttgcggtt ggtggggaga gtaccttaac 59940
gacgaacctg tatgtgacga ttgcgtggag gacaacctat gagagtaaaa tttattcaat 60000
ctataacagt aacctccaat aactggggtt ctttactagc ttatttgatt aaggaggaat 60060
gggacaaatc cttcttctcc ctagacccag aatttgagag tgagtcggta gaaaagctat 60120
gtagagacat ctctattcag attctacaga cccctgctaa ctaccatgca gattatcaga 60180
ctaaatttat agaaggtttt gggacattct atcaggtaga cgacccttgg agccctgacc 60240
aactaaccac ctgggtatgg tggagcgaag acaaagagca catcctagta gcagtagaag 60300
aaccagacga agacgaggta gaggcagaat ggtagacttg tcagagtata agaaagcagt 60360
aatcaacttg gtaaaagcta gacgtaaggt catgaaccta cgtcgcaccc aagaagaagg 60420
tactgtaggt aataagctat ctcgcgaagc ctataaagca ctctcacaac tagaagatgc 60480
ttatcaaggg gtatctgctg cacgtggtga ggttgtcagg ctggagcgca ggattaaaaa 60540
ccaactattt agagagttga ttgacacaga aggagcattg tagtgcgact tacatttaac 60600
gcgaagttag accctaacgt aacctactgg gttacgtcag acctacactt cggacacaaa 60660
aatatcctta acttctgtcc agaaacccga ccacaggctt ctgtagaaga aatggatgag 60720
cacctaatta atcactggaa ctctctggtg aaagagaacg acgtgatttt ccacttaggg 60780
gacttctcgt tctacggagc tgagaagacc aaaaacatta tcgaccgcct aaacgggaag 60840
attgtatggg ttcgtgggaa ccacgacaag agcttagact ctgtcctaca acggtctgga 60900
gaccacatgc atgaatacgt agagtgtcgc ctagacggca ctaaactatg ccttatgcac 60960
tttcctttgg ctgaatggaa ccagaaacat cgcggggcta tcatgcttca cgggcaccaa 61020
cacggtagcg gacctgggta cccaggacgt attgctgatg tagggtatga tgcttgggga 61080
agtattttaa ccttacgtag cgttgtagcg tacattaaag cggataatcc atttacccaa 61140
gaagaacccc gagaccgtgg ttagggagag cgtatgatta catcatcttt gggtctaaag 61200
atttctttag gcctattact cgtagcgtca gccaccacgg ggtggtttgc gttacaacac 61260
tctagagcgg aaactgctct agcagagaaa acggccaaaa tagaagccct acagttagac 61320
ctttcagcgg ctaatcaagc actagttcag gctaagagta gggagaatcg cctgtatgac 61380
ttacaggtcg attacgaaaa ccgactatcc gatttacgga agtccttagt gcagaagaac 61440
gaagaaatca accagtacaa aggtaggcag gatgtagtat atgccaagcc aggactggta 61500
gagcgtctag agcagaaggc tctagataaa ttctttacgg aggtggagaa tgcgaattaa 61560
agcgttacta atagggctac tgttgctcgg tggctgtgcc tccgcaccac aagagcctag 61620
ggttgaggtc agtaatgatg ttgtattatt tcacccagta atcccggaca accccagtaa 61680
cccaggactt aaaattaagg ccattacctc cgaacgcatc gctagtggag aactgccgga 61740
taaagcgtat ataggattct tatacgatga ctggcttgag tttgctaagt ggatgaacga 61800
gtataaatcg gttaatgaag aacttagaaa agcgatacaa cagtatcggg agcaggacac 61860
tagatatgac caagatagta attagtaatg aagaccttca agactttcct acggcacagg 61920
cagtggtagg tcttgttgcg gctaaaagga aagaaggagc tattaccttc ttgaaaaacg 61980
tacagggcaa gcttaaagat aactggctta aagcatgccc caaagacgaa attgttatct 62040
atgggccgaa gccccctgaa gcagccctag agtatttgca tatgatggag tacttagtaa 62100
gggtagaaaa ctccccttca ctgggctcta taggcccgga gttcaaaatt actatcaagt 62160
ggagtaagta gtatggtaga gttcatcact gcaactattt ttaagtacga cgggaaggta 62220
tactcagccc cgaaagctgc tgtagactcc tataagaagc gtaagacttt acgcaaccta 62280
ctttcacgtt cacgtctgtt agacgacaca gaggctagta cagtactagc tactatgggt 62340
agagttttag tatttgattg tgatggacta atcgtagtgc gtaccagtaa aaagactaaa 62400
gacgacctaa aaatgctaga aaaggttcac gacagagtaa ctatcatgtt ccgcagtaaa 62460
ccctgtaatc tacgggttgc gaaagattta cttaataaag gactggactt gcaggaattt 62520
cctgactatt ttggcacgtt atacatgcac cccgacttta ccgagcttta gctcggtttt 62580
gtcgtttcta cagttgccaa aaagcccaaa attcgatata ataattatat aaattgagat 62640
gagggaagat tatgcaagtt tcaaccgata tattcatggt agtagaagta caatccgagt 62700
atgtatacgg tagggaagga gtcaaggaag tatctagaac tactgttaaa gtcttcgacc 62760
aactatacgt agggaatgct gcactagcta acgctgccaa ggcagaagca gagagactaa 62820
acaaagccca cggctgtgat gttgtaaaat acgtagtaga aacaaaacca cgataaggaa 62880
gactatatgc atatttactg tgacggttct gctacagcta ctaccaaaaa gggaatagct 62940
ttaactgcag gttggtcagt agtaacgaac tctaaagtgt tttacgggca ctgtaaagcc 63000
cataccactg cgagagagaa tactactaca gccactatcc ctgaacttac agccttactc 63060
tatgcactaa agatagctca agagggtgac gtaatacata cagaccaaga gcgtttgcta 63120
gatgagttac tagtaaaggg agctttgggc gggggtaagc ttttaaagct agagcagcct 63180
aagtggaacc ctggacgctc tagggttcag cacctaaagt tttacatcaa tgagttgatg 63240
aagaagaaac aaggggtaac aatacaattt actcctaggg agcagagcaa atactctaaa 63300
ggtaacttgc catgttattt ggcagatacc tttgctaggt tgggaagaga ccaacatcta 63360
cctggcgaag tactaacaaa aagattaaag gggagaaagc cagtatatgt ttgatgcaaa 63420
tctaagagca cttccaaacg gtcaccgatt ctttgctaag gtcgtttcag ggttcaaagg 63480
gtacgacgtt accggagagc tgcatcagga caacattgtc gagtgtgtga tgctagacaa 63540
cagcgaagat agctgtgaga tactgctagt aaaaccagga ctctccctaa gagaagcagc 63600
gatggaaatg gagtacctac tagtatatga aggtgactat gacgtcactg gttttataga 63660
tgaggactcc gtaattatag ctaaccaaat tatcaggaac aagctcaaat gaaaagacaa 63720
gtaaaaacaa aaaacaaccg cagggtttat ttcacccgag ttcttaagag acgtaagtga 63780
tgaagcctcg taaacagtat aagaagcgca tgtctaagct gcgtaagcag actaagagcc 63840
taggtctaga gaactatata gctttactaa ttggcgtaca gcaagccaat tatgatagaa 63900
gactctctag gaagaaaggg gctaaggtgg agttaggaaa aatagaaaaa tccacacttt 63960
tccatactca acgtatttac tacagattgc cggacggacg tttactaaag agctatggct 64020
accgcatagt atggtaaaac attaatagcc aattctctaa taatttcgta taatatttat 64080
ataaagtgaa ttggagtgac tatgaacgat aaactacaac agcttagtga taggataagg 64140
tacttcttag cctccccagg ttgggttgag ttagacagta gaaccatcca agacctaaag 64200
gagaagtttg gactagtgtc tatgtgcagg tataagttcc aactacatct aaagcatcgt 64260
gaaggggaaa tcgcacaata cctatttata atgaatagac ctaaaccgta cctcgcgtta 64320
ttgcaccaac tactgtgcac aaaaaacgta caaattagac ggttggcgac aagttactac 64380
agctcctatg agtgtatctt caaaaactcc aaaaagagct ttactgttca cagtacccca 64440
actcgtttta gggacatatc tagaggacgt gaccgacaag tagaccctct actttcttgg 64500
atgactcctg acgagttacg tattttcact aagttcgtgt ctcgtgcctg cagagaacaa 64560
gaggagcgta agaaaagaat acagaagtac ttgactgagc gtgcaggccg caatagccgc 64620
caacagatga tagacttatt gccaggagac ctattatgaa aggacaaatc agagcattca 64680
cctgtatctc taaagacaga tacaagccaa agaacctaat agtggttcta gaagtgaagg 64740
acgaagtagt acgttttgca tacctaggca gttcttctcg tcaccgacgc agcatgacta 64800
agagcagctt cttacaacaa actgagaggt atttctagtg gttagaggtc gcactaagct 64860
aaacatactg caacagaacc ctagcagtat gtttcttggt agcctcctag ggctacaagc 64920
ctatgtagct tatcataaag gacaagtgga caagcttaag tggtgtttcg gaacactgcc 64980
agaagcacaa atacaggtga cagcagcaag acataaacgt tacctacaca gtgcgtacgt 65040
gtcaggggta gagagcactc cctgcaccaa cgacgaccca gtagaggtat cgagaaaatg 65100
aaaatcttat ttatattaat atttacgctc gcgttgtttg gatgcgatac gtcacctaga 65160
aaatccggtg gtgtgagtac tttcgcaggt aaatgtcctc caggtcagat gaaagaaatt 65220
gactctaacg aatgcgaaga catcctagat gcactagacc tagacgaaga ttgggactat 65280
gataagcgaa agaaggcggt gaaaccgcct agccctaaga agaaacaggt caaaccaaaa 65340
acaaagacag ccactaaggc agtacctaaa cctaagcgag tagttaagcc taagaagcct 65400
acctacaaac ctagagacac ataccgagct aagcctaggt ttgggtcagc taaaaggagt 65460
aagcgcaaat gattaagaag ttattgatag tagtagctat actgttaggt atcttaacaa 65520
ttagtgctgt cgtggctggg ttattttacg gtactatagc gtacatcata ggcagcatgg 65580
taggagtgat ttaatggaaa acaaaattta cgtcttctct gcttcagagc tagaaaaaga 65640
gagaactgac aagctagtcc ctggtatgtt atactgggta tctaaagtat ggtcagaaaa 65700
cgtagtgatg tcccctactg gaggtactga ccgatgtacg ttcgctagct tagttcctct 65760
agacgaccat gggcaacccc tacctagtgt ggctattaca ctcactatcg actggtgggc 65820
tactgaccta cgactcagta agtacaaacc tgttggtgag atgcacaaac aaaccatctt 65880
cttgccagaa ctactaggct gaggtaacta aatgattaaa acagcaccta atacacttct 65940
gcaccgctac ctgagggaat acatgcctcc agcagttagg cagtatgcta acaggatggt 66000
agcagcggaa gtcctaataa aagtgactaa tctacagggg cactacttaa ctgctgctgc 66060
cctaggtcta gtggttaagg ctgagagata catcagacct tcggatgatg aatgtatcta 66120
cgcagtacga atatcagaat ttcacaaaca agggattcgc ataccaaact ctacagtacc 66180
tatagtattc ggggcggatt gtgtaacaga acttagcaga gaacctggca ggtagccagt 66240
aaggaacaaa attgagcaca ctatcactag cacaggaacc ttaccgtggt ttctacttct 66300
accacttctt actacaggta ggggtggcac tagacatcga tgtctttgag gaagacgtag 66360
taaaatccct agaacatcac cctaaataca ctgactggtt acagaaggtg gccagtcaag 66420
acggcactga agagtatgta gtaactatag ctgactactg caacgctgca caaggcatcg 66480
ttactcctga aatggcagtg ttcttagaag ccctgcttca agcaagtgac caacaagacc 66540
atacagtgtt actaatcagt atgcactctg gttccagtga ttacctaaaa gttagcgatg 66600
cgaaagacat gctagcgttc taccacaaac aaccacaata aggaaaaata atggccttag 66660
ttgatataat tggtgagcta aatgagcaat cccgtattaa gcgttgagct tgcgcgtgaa 66720
gcgcttaaac atggcggtgt ggtttattgt aacgatacaa aagaaaacaa ccgtacagtt 66780
atgttaaaag aagaaacaac gatatggaca cctgaagatg gtgacgaacc tgcgtttaac 66840
tttacgctgt atcagaaacc tttcaagcta actgttagag cacgcgatat cggtcgattt 66900
acgtaccgtg aaaatgttca ctatagtggg cctaggtaat actgcacggc tttaacgata 66960
caaaagaaaa caaccacaat aaggaaaaaa taatgggcga attagtttgg attgcactaa 67020
ccactttaca accttaccca gtgttcgttc ccctagagga acacatccca ctgagagcta 67080
tcaaactctc tgaagacaag atgcaagtaa gcctagagca ggtgactaga atctatcctg 67140
ctgttgcgga catcttcact aatgaaaact ttgttatctt ctctggagcg gagttcactg 67200
aactaccagt agcacacgat ggagaaatag tagagagctt agagcggtta gctgaagctt 67260
cccgaaaagg ggcagaagct atccaatccc tgtcagcggc tattgcggaa cttcgttagc 67320
ctaagagcac aagcccctta attggggctt ttgtcgtttg cttcgaatcc aaattttcgc 67380
tataatattt atataaatta aatgagaggt tagttatgca ctatagagaa gcgtgtcaag 67440
tcctagcaga aggtgagcct gccccagaag atatgtgtaa tatagcttgc ggttgctgtg 67500
caggtgagtg gtcatctatt cagtcctcta tttaccgact aggggatgac aagcctactg 67560
tatacaagaa ctacgtaggc caagagaact gtgagtatgt tctaagcaca gcaggtggat 67620
atcaagagat gtaccaatgc ccaaactgta gcaaagacgg agatgtagtg tttctctata 67680
acatagccga atcaggcaac ctaggccctc acgataggta tagactagcc cagttgatag 67740
tggaagagca cgacctacaa aataggctca aaagtgttac agaagaaatt aggcagtaca 67800
cataggagag ccccatggac ttgaaagaat atgataaata ctttgcccac tttgttaagg 67860
taactggagg ttccgcaaaa gcagaatcaa ctagaatgca cttccagaga gcctacgact 67920
tacacaaagg tgactggcaa ctcacgattt cgatagcggt agctatcgat tccgcttata 67980
aagcaggact acgattttaa cggagattac taatgttttt agccaataaa gactaccaca 68040
caatcctacg actagagcga ctatggaagc agtttcatgg aaaatcattt aaatctatgc 68100
accccgatga aaagtgtatt ggttgcttcc taggagtgga aggccactca gcttgggaag 68160
atgcggaatg tgccaaagta tcctttgagc agaaggacaa cccattcact aagcgcaaag 68220
atatcgaggc tattatacaa gacctagtag accgtgaaaa agaagcgggc gataagaaca 68280
ggcgtgatga gatgatgcaa aagtcaaaga actacaacga cgagcaagca gcagatattt 68340
aagaggtaac aatggaacaa tatagacacg cgctttacca taaagtaaaa gaaacactaa 68400
aacaagtcaa gactggagag gtacctttaa cggagctaca cttaatgtta ctagggttct 68460
ctaagagagg aaaacagtca ctagccaagt cacaaagtac ttggcaattc tcctttggta 68520
atgcaccggg aggtatagag tacatttgca ggtattgggc attacacgaa gaacgtgata 68580
actacttcct gaatccagag tctgtaggtc taggacgtgg caagtctgac gtaggcactg 68640
aactctcctt ctctagtgga gatactacac acattaagga gtttgtgaga aaactacctc 68700
aatggccaga gttaggctac aaacctaact ggggcaaatg gaagaagaac tctgagcttt 68760
ctaatacttg gaatactcct gtcctcgctg tgttgtacga gataatatat gaaaaaccag 68820
tgcatatcat gcaattggta gaagaactta agactcgcga gtttgagcct acttgccaat 68880
tcaatttgtg caatggcaat ccaggactta agcaccctat actaactaaa gcttctagag 68940
ccgaacagat tgaagatttt aggcagggca agccggccaa gcttcgcgag actaaggagc 69000
ttatgaatga gaaaccaaaa atcggaatga ccatcaaact caccccagag tagaaaaagt 69060
cggttggaaa aacttcaaaa tatgttataa tcatatggaa cttttagaga ctaggaggtt 69120
ttaatttacc tcctttctaa aaaccctagc tcgcttcgcg aatgcctagg attcttgttt 69180
ccattcggga ggcggcagca aaggagagat ggaaacaaca aaaatcctag tagtattcgc 69240
aaaaaagcga gcaataaaat actactaaat atcttttaat aacttcttaa tcttttggtg 69300
gggactctaa taccctaaat tccactcgaa ccttaaaaag tcatctttta ctaaaaagag 69360
actcctcaaa aacagactac cttaattccc acaagagact tttacaaaac aaaagccttg 69420
cccgccccgt aatcccttcc tagtatcttt ccctataatc cattcctagc ttctaaaact 69480
taataacctc tacatagaat ccgcactcac ataactttcc ctagaatccg cactcacaaa 69540
cccattcaaa aatctaccct agctcacata actttcccta gccaacctat ccctagcctt 69600
attgtaatta caggcactta aattacaggc acttaaatta caggaacccg acggctttgc 69660
cgtcgaaccc cctgagcccc aggttgaatt ctatatgtac ctaatcctgg ggagctgcca 69720
gctcctgtac tccacaggtg gcgcggcttt tgaggcccca agggcgcaga atctcaccga 69780
aacccgattg tcaagcgatt atttcaagtt tttgccgatt acctccaaaa acgccccagg 69840
gcccatgtag ctcgacggcg tagccgtcgt aacccacgaa gcttccaata gaatccgcac 69900
tcacatagaa tccgcactca catagaatcc gcactcacat agaatccgca ctcacacgta 69960
acttttgcac tcatagattt cgcactccct ggggcgcaga atcaatgtac cagagaagtt 70020
gaatgagaat tattcaaaga gcgaaatgat aatcatttgc aaatgcgaat aattctcatt 70080
tgcccctgcg agtacgaatg cgaagcattc tcattcgcat tccctaggtt gaatgagaag 70140
cattatcatc tagtttactg cacagatgga caatataaaa tatggcatga attttgttat 70200
ccacatggca cgatttttgc tttatcgctg taaggctctc tgacgactta tccacacagt 70260
tatccacagc ttataccact gtataaataa acagtacctt gcacgtagtg caatattctg 70320
ggtgcccttg agaggcactg taacgctctg taagccgagt ttcaccgccc taggtactaa 70380
agcaagccta ccatccacta gcagccttgc acacgcacgt aacacaaatg ataatcattc 70440
tcattgccgg acgggcgcgg tcaaatgaga agcattctca attaggctct ggatacaaaa 70500
aatcccacac aaggcgggat tggtatgatt attgctagct aacacttaca aggtgggtga 70560
ctttgtcaaa ctcatccttg gtcatcttta ggtcttcacg caacaattct aggaagcctt 70620
cggaaaagtc acccttgggg atgtgctcaa caaaaatatt atctagtacg ttgtgaattt 70680
cttcctcgca gatttcccaa gtgtagtctg aaatttccac aagctcaaaa tctagtgcaa 70740
cgatatggat taattttggc atgattattg cctcactgtt ttaagttcaa ccagaccaac 70800
aattctaggg ttactggtca ctatgcgata atatacttcg tcccgtagct ctcggctagg 70860
gaacgcctta gtaaatcggc ggatacctcc gccgagtaca tcaactttta ggtagtagat 70920
tatttggtaa gctaacatta gtctactctc catctatgta atataccccc aaggcgacca 70980
agctacataa aatcactagt acgccttgaa acgtgtgggc aagttccgtg ccaaaataaa 71040
ttctgactac cccaaacaat gcccctagaa caaatacggc tctcaaaact tagccctttg 71100
cggtgcttta ccgtctcgca gggtgaacca aattgtgatg cacggtacta ggttgataca 71160
ccaacggcga ttgaatgggg agtaatgacc gcctaaccaa atagcgccct tattgaatag 71220
gataccgcaa cgtggttccc ctactacgtg gttattgact gcactacgct ttttcaagtg 71280
cttgcaatct acgtacgact ggcgaccttc acggatacat ttgtgaacca ttgagtccag 71340
gtctgttact tctactttaa ctaacatacc ataccaacga tgagttgggt cgtctactat 71400
agcgttatat gtatacatgg tcttttcctc gctcttagcg gttagttgtg ggcgacctta 71460
gtcgccctag ttggttagaa tggttgaacg ccgttaatga tagcttgttc tagctcgctt 71520
agtgtgtcgc cttgtacagt caggacgtga cctgaatcca cacgctccaa aacaacagtt 71580
agaccatcta ttgcattgtc agtaattgag attacagcat aatcactggc gttagtctca 71640
atgatattgg ctagggctac taggttagat gctttcatgg taaatctcca aaatctcgga 71700
aaatccgtcc ggtcggtcat acctcgctgg tatgggtata taataggaca atccgttgcc 71760
ctagtcaagt gacctagctc acatttttgt caggatacac gtcttctact tctagaacca 71820
caggcactac cttggcatcc tcgcttatat agtaattatc ttggaacctt aaacagtctg 71880
ttttgtgtgc cgatgcacta ttgatgggca agtaaactac atcctcatcc gtctctgaat 71940
aggtgtatag agctatctta acatttttta gctctatatc ctcacctcgg ataacaaagg 72000
cttgaccttg tagcaaatcg cttgccaaga actctttttt gtcttttttg tcttttttgt 72060
gaacaatcat atcctacccc tacactacgt agtcgatttg gtctatacca atttcacgcc 72120
acaccttaga gtcactataa atagaccagt cgtctagtag gtttagcatc tcgctttctt 72180
gctctctgtc gtagatacct atagtttgca tctcatcgtt gtagaacact agcccgtatt 72240
taggatgtac cgtaatcaac aaggactcat ccgagtcgat ataccatacc gtgtctgatg 72300
ggtcatccgt tgcggatagc attgacaatt ccgccagtat atagtcaaga gtgactagac 72360
tagtcttatc tttttggtta aacattttta gacactccct cgactaccac tttcactagg 72420
caaacgttgc ccgaactact gatatagtta tcagaacgaa tatctccaaa ttcttgtaag 72480
ttgtgtgttt ctttaggtac taaaggcata tatactatgt cgtcgtcatc gtcggcatag 72540
tgacacactg caattttaac attttctaat gttaattctt catctttttg tataaacgtg 72600
tcaccaaatt ttaactctct agcttccata aaaccagttt tacgaacatc attttcactt 72660
aatacaaaca tttttaacat ctccaatggg ggtaaagacc tactgcaata gcagcaataa 72720
acataacaca agcaattagt tcaaacatgt tatacctacc ttgtcaaata gtttctgcat 72780
actactagct gtagtatact cgcccgtctc tttattgaga caccaccaaa taacatcaaa 72840
gtgaatacgg tacatgttgt gtcctccttg ccgccttccc ttatatagta gcaattctag 72900
cccatttgtc taataacatt ttgttataac caccagcaaa acgggtataa gctctgtaag 72960
cgtttctgag aggttttaga aagaagcctt actttaatat gtctaggttg tggataaccg 73020
cttagaatgg attctacagc ctagaaacgg cattccgtaa tttataagtg cctttttagg 73080
gtggataact ttacttagcc tgttgataag ttgtggatat acagtacata accagtatta 73140
ttagaacttg ctaatgtgca taaagttgtg aacaaaaggc ttggcataca ttttgccttg 73200
caaaaggcgt gccaacgatt tttcgaccca aatgcaaaag gttctcatct gcgaatgaga 73260
atccttatca tttatatata atatggattc tcatctgcga cgatttttga gtttgaaatt 73320
tgtccttgac ttttcaaaga agctcgttct cactctattc actaaagtga ataaatatgc 73380
agaaaaatac ccaaaagtgc ataaactgtt tgggaatacc attgcaaggg tagaggctaa 73440
aatcgcttag aaacgcacac agagcgtttt agaggcatgt atatttatac agtgtttttc 73500
aagttggcat gatttttggg ttggagctat atcacacttt tcaccaaaat atttgatatt 73560
tattcctagc tatgatagac gcgcgcccgt ttccttatat ttatagacaa aaatcgctga 73620
tagccaaaac ctatcaatct agggattttt aaagatttaa acgattagac tcattgactc 73680
aatttgctat tatatacaca taccgcagca acaaggcggg acagaaaagc cgaaaagcct 73740
ttcgaaaaag tttcaaaaaa gtagttgaca caataaacgg cttttgagat actaacaacg 73800
ttcccaaggg aatacgctct ttaacaattt ggatatgttt tctatcaggt agcgaatagt 73860
tcactgacta ggtatttaac caagtgccta gttcggggaa ttgtcccatt actcgggtgc 73920
tcataacatc cggcaaatct gaaaagagga aacaattatg actaagcaaa cagtagcagg 73980
ttttgaatgg aacaaagaaa ctgaatctca agcaacaact ctataccgtg aacttgtggc 74040
ggaagttggt ttagaaacag ctaatacaaa tgttgagcta ggcaagattg cgaaagcggt 74100
cggggctaaa tccgctcaat cggtacgttc taaactgtcc acgcttaaag acgaagaagg 74160
cgaaccagtt taccaaaaag ctgcgaaagc tcgttcagta cgtggcaaag tgaaaaccca 74220
aaaaattcac tatatccgtg cattgcaaaa cctagctacc gctaacggcg tggaagtgac 74280
aggacgcaag tttgagtcac tagagcaagc tgtgggttcc gacttggagc taatcgtaga 74340
gctggcggaa gccctaagcg gtaagcaagt gattgttaac cctgacgaca ctcaagctga 74400
cgaagaagcg aaagttccac gccaagaaac ggcgaaagcc taatgaactg gttagtgcta 74460
ggcgtgctga taggtgcgtt tgtgctacct aacctattct aacagattgc ctccaaacgg 74520
gggcaattta cactgtaaat tgaccatcaa tttacagtgt aaatttacac cactaaccgc 74580
tataagcgag gatatttcta atgactattt ctaacaaaat cactgataat gcaactgcaa 74640
tcctaaagct agctaagggc gaaaactttt ggggcaaacg cctagactcc aagcgccgag 74700
aggtagcaat tgcaatccgt gacgatttgg agctgggcga ggatgtacat tctagggaga 74760
taatcgactt tctggacgac aaccgtccgg ctccaaaatc ccaaactaaa tcgcatacca 74820
aacaggcgaa agaggacatc gaaccaactt tcgcaggttc gatgtcaggg cgtgaaactg 74880
acgacctaga cgacggtatt tatatcatta cggcaggaca gaacaatacc catccccatc 74940
cagtgctgaa aaccttgcta tctatggcaa aaaactatgg tgctgaattt ggtatcatgc 75000
ctattaaata tacaacaact ttgcaaggtt tggagcgcaa aaagcctagc ttccatgcgg 75060
atgtaaaagc gcacctatta acctatgttg acacttggtt aggcggtgaa ggtaatgtat 75120
tactggcaaa cagtgcgcaa attttgccta ctgctaaaca gcctatcaac gcagccgagc 75180
gattaaatac tggcgagtca atcacggtag ttgctagccc tagacgacaa acaaaaacac 75240
tatctagaca gaaaaacggc tcgcaccgtt gggtatatac aacggcagct tgtacgctca 75300
aacactacac cgacagccgt gcaggtgcgg aggcagaatc cgagcattgt tacggcggtc 75360
tactaatcga agtgcgtgac ggctacgtta ctcaccgtag attggtatca gatgaaaacg 75420
gcgtaattat tgataacggc tcggtgttct tccctgacgg ctcaatcaaa gcctataatg 75480
atgtattcga aaatcaaccg gtaatcgtgc taggtgattt acactgcgaa aagatgtgcg 75540
acgactcatt cagccgtgca actgactggg ctaagggtgt taaccctaag atgatagttg 75600
tacatgatgc gctagacttt atgagccgta accatcataa ccgtgaagat tggacgttct 75660
tataccagat gcaagataga gccgtaattg aagatttaac ggatgtaatc aactacctaa 75720
accgtttggc agaaattgcg cccgtcttta ttgttgagtc aaaccacgac ctagccctag 75780
attcttggtt gcgtgataat cgctttgatg tacgaaaaga cccgaaaaac gcccgcacat 75840
accatgccct aatgcttgct tacattgaag cgatggataa cggaacattt aacgaccttg 75900
cgaaaatgga cttagccttt gcagccttag cagaccgttt gccagaactt tctagtaaca 75960
ttgagtttgg taagattgac gagcaacgcc tcgcattcgg ttatgatgta tcaatacatg 76020
gtcatgtagg tacaggcggt gcccgtggtt cggctgttag ttttaagaaa atgcgcatta 76080
agactgtaac agggcataca cattcaccgt ttgaagataa taacacggtt gtagtaggtg 76140
taacgggttc gatggaaatg ggctataaca aaggcggtac aacttgggac agagctaacg 76200
ccgttattta tcctaactct acacatcagc tagtgcctat gtacaaagtg ggcgagtacc 76260
aatactaaac cccaaaccgt gacgggcgca aaaacgtgac gcccgtcaca cctaactaat 76320
ccgcaaacat gcgaggaaat accaatgaat aaactaaacc tattagcctt atcaatgcaa 76380
accgttcaca tttttgacct agacggaacc actatagaca gctttgaacg tgtagagcct 76440
tgtttacgtg agggcggtga tttagacctt gacaagtacc gcaaagaagc gtgtgtacac 76500
aataaggtta tgcgtgacaa gctaatgcca ctagccgatt acatgaaagc cctaatatct 76560
aagggcgaaa ttgttgttat ctgtacggct cgacacatgg ctaatagtga ttactactat 76620
ttacgcaaaa acggtttgcg tgttcctctt gtccttagcc gtgaccaact acataaacac 76680
ttcacagccg agcaagtgca gcgtatctat aactcaggtg atgcagccta caagggtgcc 76740
tactttgaca tgttgcttga gcgtttcgga accaaccacg aatttatcat gtacgacgac 76800
catcagggcg tgcttaaagc agctaaagcg agaggcttta ctgctattga tgccactaac 76860
ttaaacctta tgcttgaaat tgcatatcag caaggcttta acgatatgga ggaactggcg 76920
tataatgaaa gtgaatctct catcgaatcg ctaactggtc aactgttagg tgctagtgca 76980
taagataacg gcagggcagg caatcgcatg atgtacagcg attgcctcca aaaacagact 77040
agacctgtac agggtaggcg gtaatgagac ttgttctcat ttagggcacg tggcgacccc 77100
ccttcacgta ctacttatgt gaattttggt ttggtatgtt atcggtgcta atatacttat 77160
gtgatttttg aagtaaggcg tactcggtgc taatataccc agtgctcata gaaacagact 77220
actcggcagg tatcggcaaa aacggcagtt caaaaatttt attatgtgaa atttcgtact 77280
cggcaaggtt cggcaacccc ataacaaaat catctttgca tcggcgaatt tatattatat 77340
aataatcaca agatttgcaa aaattttgat gaggagtact cgatgtcaga tgaagataaa 77400
ctacttccgg agataatggc tccggagggg ttgttagttg ctgaggccta tcttgagaca 77460
ggcactgtag cggcagccgc agaaaaacta ggcatgggtg tggaggttgt agcggcccaa 77520
atgaagaccc ccgaggttag gcactacata aactctgtgt tcatggaaac gggatttagg 77580
aaccgagaca gactattcgg gctcctagat gaaataatta atagaaaaat cgcagaggca 77640
gaagaatccg gcatagtatc ggaagatgac ctactatctg tgctagagaa agtacataaa 77700
atgaagatgg ctgagcttaa catggagatt aagcttatag aggcccaaaa caaacagaaa 77760
gcccctgcaa atcaaactaa cattcagaat aacttttcag gctccgaggg gatgaacggt 77820
cttctaggtt ctctgattaa cgggaaatca tgatgagtaa gtttcagata ctcctagcta 77880
ttgtatttag agagttacta aactctaggg cttgctggtc tatgatattc ctaattgtag 77940
gagtaatttt atgctttgta gtagggatat agtaaccgac agagtagtag acttccccgt 78000
agaagataga ttcatcaaac taccgattga aaactatctt gctatggaag aaatcgacgc 78060
catcgcaccc cagctcgcga taatcaacgc tatcaacgac ccgcaatacc gtttcgttac 78120
gggggtctta agtagacgta caggtaagac gtacatcggt aacgttattg ccttccttaa 78180
gctcctagag ccagggtctc aggtacttat catagcacct aactactccc tagcccagat 78240
ttcttggaac gagcagttgc agcgaataaa ggctcatggc ctagagatgg aacgttgtaa 78300
tgcaaaagac aaagaactag tgctagaaaa cggtagcctg ctaaagcttg gttccgttgg 78360
taacgcggac tcgtgtgttg gtcgctctta cgacttagta ctgttcgacg aagcggcaat 78420
cgatgaccgt ggtggtgatg cgtttaacgt taccttgatg cctaccctag ataaacctaa 78480
ctctaaggcg ttgttcatat ctacccctcg tggtacgaac tggatgcatg acttctataa 78540
caacgggttt agtcaagggg ctgagtttgc ttcatggtgt tcggtattcg gtacgtataa 78600
agataaccct agggtttctc cagaggttat tgcgcaagcg cgtgcagcga tgtctaagcc 78660
tcagttcgag caagagtttg agtgttcatt cagcactcgt gaaggacaaa tctacgaagc 78720
gtttgaccca gacaagcacg ttaaagacct tagtggtatt aactttaata atgagtatga 78780
gtatgaaggg cttatgggta ttgacccagg gtacagagac cctacggcag ttctttctat 78840
taaatatagc tttgacgagg atgtattcta cgtagtggat gagtacatgg aggccttgaa 78900
gcctacatcc taccacgctg agaagataca ggaccaaata gataggtgga tgatagacat 78960
aatcaccgtc gactctgcgg cagcgcagtt ccgacaagac ttagtgtccg aatttgatat 79020
ccctagtaac ccagctaata agtcggtgct agatggtata tcctatctac agatgctatt 79080
ggagcaagat aaactcataa ttagtgataa gtgccacata gtagtggaca tgctaatcaa 79140
ctaccgttgg gatatgaatg agaaccttgt acgtgaacgt ccagtccacg atgagcattc 79200
tcaccctgcg gacgcgttac gctatgcact atacagttac tcaaggtgat agtataaaat 79260
cccctagaca tatttgttat gtgatgtata atactaacat gaattgaatt tggagcagca 79320
aacatggcaa aaaatacagg actgaaacgt gacgccgtta agtacatacg agacggaata 79380
aaatcacaat acaaaaagaa ggactgctgc gagatttgtg gaactacaga agatattgag 79440
cttcatcact atcatacagt ggctttcgta ctagaaaggt ataccaagga gaaggggttg 79500
gacctctcca atgtagataa gatactcgct atgcgtgagg aattttacaa agataaatgg 79560
tatgaactag tcgaagacac tgtatctctc tgtaacactc atcacgtgtt gctgcacaaa 79620
atctacggtc agaagcctct actacacact gcgtcgaagc agaggaagtg ggtagagaag 79680
atgcacaata aggctaacgg aattgaagac ctaactgagg aacctacggt cagttacgaa 79740
actgctctag gtacttttgc ggtcaagtcc aagcctttaa gcaagttttt ggtataggta 79800
tgggtttctt tgattggata aaggaaaaaa taaaccctgc acagccacag atacagcgaa 79860
gagaaccttc aagtagtcga agcaataggc gtcctactac ggtacgtaac tctttccgag 79920
atatggagat agttaaccgt ggtgttaaca tgctaattga ctcctgtgca gaagtagact 79980
tcgatgtcca aaagtctcgt ggctacacca cgtatggctc aggcatcaaa gggaagacct 80040
tagacagact gctgaaccag cgccctaacc tattcatgga ctcaagtacc ttctggcgac 80100
tagtttacat ggatttctta ctagagggct gggcattcat ttactacaat gaagaagaac 80160
aggctatcta ccaccttccg gcagcgaata tggaagtatt tgctgataaa aaatattata 80220
ttaaccattt cttatatgat ggacaaacta agtacaagcc taatgagatt atcttcataa 80280
aagataactc cttcaacgga gaaacctccc agatatcagg ctaccctaga gtcctatcag 80340
cactaagtag cattatccga aaagataagt tgcagcactt caaagaaaag ttcttcgaca 80400
acggaacagt tatgggtctt attatagaaa ccgagcaggt tctgaataag cgtctaaagg 80460
atagagagcg cgaagaaatt cgcatcgacc ataacgttag aaatggtaaa tcaaacgttc 80520
ttatcctaga tgggggtaag aaagctaagt cccttagcgc aacgaattta aaagagttag 80580
gtatcgagga agacctagat aggtttgaca aagagatttg taaggcatta ggtatcccac 80640
caatcttact agagggcggg aacaacgcta atattagacc taatattgac ctattctact 80700
acctgactat tctaccaata gctgagaagt ttgaaaaagc tttagagttc ttcttcggtt 80760
tcgatatcaa actagacacc tccgatattg cagcactgac acctgaccga gagaaggagt 80820
ccaatgagct ttcttctaag gttaataatg gtatcatcac aggtaacgaa gctcgtgaga 80880
aactacgact agagcctatc gctgacccac taatggataa gattagaatc cctcaaaacg 80940
tagcgggttc tggtactggt gtttctggtg aagagggcgg agcccctaag aaagaggata 81000
atagctaatg aacaaatcaa tccctttcga taaaatccaa gaagtatttg gggactcact 81060
cccaacaaaa gctcactttt acgcatatgt agcacatcac ggtgttacac agcttatggt 81120
agagaaagcc tataatacgt gctcagtaaa agcttggcac ctactagtgg cagacttcga 81180
agaatttatc gagaacaaag aagaacctgt agttaaggag cctgtcaaga ccgaagcggt 81240
cgaggaggat tccgatgagt aacgttctag acaaacgatg tgaaaagtgg ctaaattcca 81300
cttttgacgt ggttaagaaa cagaagaacg aagacgaaac ccgctctatt gttatctctg 81360
ggtacgcgaa tactaccaat gaagacagag caggagatat aatcccagcc gatgcatggc 81420
ttaagcccaa ggcaatggaa aactacttaa agaaccctat agtacttttc cagcacgacc 81480
atgacgagcc aatcggtaag atgctagatt ataaggtaga cgagaagggt ctatacgtag 81540
agattgaaat ctacgatgta gaccaacgag ttttccgtct agtagataag ggtgctctta 81600
aagcattctc cgtaggtttc cgaatctccg actacagcta tgatgttgac gaagacatct 81660
ttacaattac agaactagag ttatttgaaa tctcagtggt ttcaatccct tgtaaccaag 81720
actcattatt tgaggctcag aaatctctag actccacatc attcaaagct ctaaaagaag 81780
aaatcagagc agagaaaggc tctaccccta gcgtagaacc ggtttttaac agtgaacttg 81840
aaaagctagc gtatgcgcta ggatatttta aacaggatta ataatgtcta ccacaccaaa 81900
tattgacatc aacaaactta aagctgagct taagaaagac ctaggcctag acgacgtcgc 81960
ggacctagta gcagagcaga agcgccaaaa gcaactagac gaagataagc gtcgtcagga 82020
agctgaaacg aagcgcatgc aggaactagt tgacaccgct acaggcgaga tgagtgagaa 82080
attcgaaaaa gctctacaac ttatcgacgc aatggaagaa gcagctaaga aatcggctga 82140
agacttcgcg gctaagctag aagaaaacca agaagtagtt acagaactac aatcagaaat 82200
taaatctcta ctagcacgtc gtgacggtaa agatatcgta gctacagcag ttggtaaaac 82260
aattcgcaac gtagaagacc gtgaccagct aatggaagat gctgcgttcg ttaagtctat 82320
catgggtgtt gactctgtag aagacactaa gttcggtgca gaagttcata aagcagtaaa 82380
cggtgcttct tctatccaag tttcttctga agactacgaa acagtattct ctaaccgtat 82440
ccttcgtgac attcaaaagc gcctagtagt tggtaacctg ttcacagaac taccaatgac 82500
ttctaagttg ctaacaatgc aaatcgaagc gggccgcgac gcagcaggtg caacatgggt 82560
tgacgctgca cagttcggta ctgataactc taccggtggc gagcacacca ctgcacttac 82620
tgacattacg ttcagtacgt acaaactagc atgtaaagct tacatgactg acgaaactac 82680
agaagatgcg attactcctc ttctaggtat catccgtcgt cgtctaatcg aagcacacgc 82740
agaagctatc gagaaagcat tccttacagg tactgttgca ggtgctccaa tcggtctagt 82800
taagctagct aacgatgacg gcaacgcagt aacaactgta aacggtgcag atgcagcgtc 82860
tctagttcct gctatcgacc tattgaagct tcgtcgtaaa ctaggacgta agggtactcg 82920
tctaaacgac ttgactctaa tcgtttcctt agatgtttac tacgacctta tcgaagaccc 82980
agagtggcaa gatatggaca aagtaggtaa tgctgctgct aagctacaag gacaagttgg 83040
tcgtatctac ggtctaaacg ttgttgtatc tgagtacttc cctgctaaag ctgcttccgt 83100
agcatgtgcg gttctagttt acactccaga cttcatcgta ccacgtcagc gtgacgtaac 83160
ggttgagtat gagcgtattc agcgccaaca aaaagatgct tactacgtaa cacagcgtct 83220
gaacctacaa cgttacttct ctggtgacaa cgtagcagtt cttaactacg ctgctgctta 83280
agcgtaacac tgaaagggag tagggctgag gctctactcc cttttttctt ataaggagag 83340
aatatggcaa ctctattgtt tagcttggaa gagtataaag aatacgctgc tatagcatca 83400
ccaactcagg atgcaaaaat agagcaacta atgcctgctg tagcctctgc catacagact 83460
tacttaggtt acgagttcga agaagcccca gtagctaaca gtaatagtac ttttcctttt 83520
gtcacggatg tactagtagg tgctacggaa actcaagtta cttctccaca ccaacaggag 83580
ttcatgttgc aagatgtgga agtggcagtt acagaggtca agttacgaag aacaggggct 83640
cccgaaacag agaacaaagt tctcggagcg gacgactggt atgccgaccc taggttaggt 83700
aagcttactg tgtttcctac actagataag gcatatatta tgaccgttac ctacgatgta 83760
gacctaatcc aggcgtctgc tgatattaag ttagccggct ttatgcttct agactactgg 83820
attgaccgta agaatttcaa taaaacagtg gttaaccaag gtcagtctac taccaaggta 83880
cgcctaaaca atatgccaaa gcatattgag aacatactta acatgtatag gaaggcttaa 83940
tatgtcagga ctttcggacg cagctatggc ccttactagg gccatatacg aagatgaggg 84000
cggaaagacc cgaggaaagg tagcttatga ggtagtcgtc gcagataccc gtagtgacac 84060
aactctcata acactgaggt tcgagtctaa ggccccaata aatgaggatg agttacgagg 84120
caaggctagg aaggctttca ctaagcttac tgaagaactc gcggctgacg ccgcagcgga 84180
cgtaggtgct aagttactca ctcaatacgg attcgaggta gagggggctg gccagaacat 84240
cttaagaaaa agaacaactg gtagtgttaa tatagtctat gatgagacag gcggggaaga 84300
cggaatttcg gcaggtattc aaaccgctag aggtaagcta atctcccaga agaacctaac 84360
gttcctactg aatctagtaa tgaaggagta tatgttgaag gaaatgactt caccttctgc 84420
aggcaaagga agaaatactc cccttagaaa taggacaggt aggtttgtca actccgcagt 84480
agtggataag gtagccttaa ttaacacacc atcgaaaaga caaaaacaaa aattgtcttt 84540
atattttaat tatatgattt atccatatca agttttcgac ccacgcagga cctcccgcga 84600
ggcactggca tcgaaggcta gagaccctgt taggataatt ggagaagcgt tggccaaggc 84660
tgctagggac ttagtcttag agaaggccta caacatagag gttagacaaa tatgagcaat 84720
ggaacacaca ggacagcaat cgccagtaag tttgtagaaa taatcaaaga taactttaca 84780
ggaactggcc cttattacac caacgtgtat ggtagaaatg cctctaccaa aatcctacac 84840
tttgatgaaa taaccgactt cccctttata agtgtagtta aatctaccga acgcattgag 84900
aacctccctg gaggctttaa ctggcaccac tataatatgt tcattagagt atatattagt 84960
aacatagaag actatgacga ggagctagag aaagtaatat ctgatttaat ttctctcatt 85020
gacaatacgg aagattttga atatactata gaaaatcctg atagttcggt tactactcat 85080
cgagtaactg aggtggtagc cgaagagatt gggacggacg agggattact agccccagac 85140
gcaatgggtg aaatcagact gcgtgttaga tacgagacgc agaattctag attctgctaa 85200
caacatttta ggagttatat aaatggcagt taaattactc cgtaataccc gtctgtgggt 85260
aagtacggtt ctatcaggtc acgacacaac caatacttgg gaaatccaag tgcaggacga 85320
cctatccttt aaccagaacc ctacgagttc tgatattgaa ttagaagaag caggtgcaac 85380
acctacacgt ggttccgccc gcttcaatga cgcactagag ccagcggatt ggacattctc 85440
cacttacatt cgttcgtatt tagtagaccc agatggcact ccagatagtg gtgacgagta 85500
tcagtttact cctgatgcac ttctatggca ctccctagct tctggctctc cattcgacgt 85560
tactagtgca gcaggtgttc attctaaccc tgttaacatg cttctagact ttaaagattc 85620
tcaacaccac gaactattga agttccatgt ttacatgaat gtagatggtg catggtacgt 85680
tattgagaac tgtcaagtag gtgaagcaac aatctcaaac gatattgatg gtattggttc 85740
tactacatgg actggacaag gtactttact atcagagcta ccttcgcaac catttgaccc 85800
agcaaacgtt ctttctgtag actgtaactt acaagcttct tacatccgta acaagctgac 85860
agtcctacga gttactgata acagaaattc taacacagcg tatgacatcg ctatcactgg 85920
tggttctatt acatttacca acaacatcac gttcctaact cctagtaccc ttagctgttt 85980
agacgttcct atcggttcgt acaccgggtc tctatctatt acaggtgaga tgacagcgta 86040
cctagacgac aagactaacg ggtctaagaa gttactagcg gacatgttgc aacaaaaatc 86100
agtaacatct agttttgaga ttgcggtgat tatgggtggt gtggctagct ccggtacggc 86160
tccagcagct gttatcgttc taccgacagc gcacctagac gtacctagta tcgaatccgc 86220
tgacgttatc agtaccagcc tatcctttaa gggtattcct actgacttct cagcagggga 86280
cgaggccttc ttaggcttct ctgaccgatt cactagtgca gaaattgacc gtctaatcaa 86340
cactggagac ggtgaagctt aataggagtt aaagtggcca gcagacaact tttaagaaac 86400
gccctagtgg ttatagtcta cgaaggccgc tcttacactt accaagcagc ttcctcaata 86460
agcctagaga cctcctttac ggaggtctct ggtcgtagga agactttatt taagaaagta 86520
gccaagaaat actctataat ctccgagaag gcatcaacta ctgtccaagt agacttagtt 86580
gctacagacg ggagaactga ggagctacta ttcaagctta taggcctttc agaaggtcct 86640
ttacaccgac ttcctgattc tatcccagta agtcctgaat acttcgatgt gtacctaaca 86700
aatactgaag taactattaa gttatcccca tgtttcttag agaccataga cgtgtttatg 86760
agtaaggatt ctcctttagg tataggcacc gtgtttaatg ccgcgagctt cgagtacgtc 86820
gatgagatgc cacaactgga aaccagccca caaggactac ctctaccatt aacgcctata 86880
tacctaggct ttggtgagca gatatttaac aatgtggtaa acgcatcctt cagcatacag 86940
caagtagccg actggcgaca agataaatct ttatttaccc ctggaatata ctctagaggt 87000
aatgcaatac tagaagactt aatagtatca gctactataa caacaaactt agatactaat 87060
ggcttcttta ctgaacccac tataggggat ttctacgtta agcagaacac tatgtctttg 87120
agcatagact ctgcatctat tatacctaga aatactatag gaactgttta tcaaaataac 87180
ttggacgtat ccccagtaga agaatctgga gacgtcatta ttcaatatgg agaaaaacga 87240
tgagccttaa tttagctaac atcatcctag acacaaaaac tatgactttc gaccacccag 87300
aattcgaagg tctaacaatt actctaactt acaactctaa gtctcgccta agtgagctac 87360
gtaagagttg tttagagaca aaatacgacc catctgttgg cgcaccaatc caaaaactca 87420
acatcgagaa gtggaatgag gtattctgta aagaggttat ttcaacgtgg agcggcttca 87480
agtataagta cctagcacag atgctactaa tcaatgagtc agccgtggag ctagaagacg 87540
agctagagtt cactgttgag aacgctacca ctcttcttac gcaaagcaac acattcgaca 87600
gctgggttaa tagctgttta gctgacctta acaactttcg aggctaaagc tagagaagag 87660
gtgtttaagg ggctagatac gctccttaaa acccaacaag gtctaaccaa agaaaaatac 87720
tacatgatat gtgagcagtt ggggcaggaa cccgacccta acaaggtacc taaggacctg 87780
tcagatatgc cacatgtcgt tcaaattgct aatatcatct acaaatccct atccgatgtg 87840
tttatacctc tcggaatgac aggctctata ttcggaggta aggactactc cgccctccca 87900
gtattcttcg atatatatga agtaaggagt cctagggata aagaattagt gacagatatc 87960
atattacata tggaaaagaa agctatagaa caggcggtag cagaacgaaa acgcaacgct 88020
agtaagaaat aattcttttc tccatcttag cccccacggg ggcaattttt taaaggaaac 88080
ataaatgtca acttcattta agaatatgac cccggaagct aagcgagcat atattcaaaa 88140
ggatattacg gatgctttag acgaaagtct tgagagcacc cgtaaagcag ccctaaacca 88200
gatgactctg atttctcaaa tcatgcaaga tgtagagtta gaatcgggta gagtgctgca 88260
tccatacaca gacctgcctg tcgcagggca gacgtccttt gttatccaag gactctcaat 88320
cactaagact cagacagtag ctgtggtaga cttagctgat gaaactattg aagacatgaa 88380
tcctatggta ggtcctcgtg attgcctaac aactttgcta ggccaagtac tagctaaaga 88440
catggagaag aacttcgtag acgctgctag ggccgacgca accattccag tagctcctgg 88500
tggggccgct acctgggcag ggttaaaagc aattatcgca gatatggggc ctaacgtgtt 88560
caatactgtc gggccaatta tctttgccgt tagcttagct gactacttag accttatcag 88620
tacagatgcc cttagcgacg ccaaggactt cttagataag aaaattcgca tcgtaacctc 88680
tgagcattta gcagcgggtg aggtactagc ctaccacact cacggagtag ctggagggtt 88740
cacaccacta actatcggat tcgaaaggga aggtggaaaa caaaacactg cactaatcgg 88800
cggttatagt tacgggtacg cttgggatac taaatacatc cgattcgtaa cggcttaatt 88860
atattagcgg ccttagtgcc gctattttta taggtaaaat atggcagata acaaatcagt 88920
acaggaatta atgattcagg tacgtagacg tggtataaag cccgccacta aagaggtaga 88980
ggctttaagt gaagcattga atgatgctgc tatattcacg gatgacctta ataaaagcct 89040
ctccaacata aaagttcctg cgtccatgac ttccctaaca ggctctttcg agaagttcaa 89100
cgatgtatta gagtacatag aatcgaacac ctttaaagcc tctgaaaccc tagacgctgt 89160
actagaccag ttaggtgaga acgcagacgc tgcacgtgca gatatagtag gactatcaga 89220
agaggttcaa gaccttaaca ggaactccgt agcaacgggc aatgcagtac aaaaactgga 89280
taataatgtt agaggcgttg gtgagtcttt tgagcaaaca gaagggcaag ctagacgtgt 89340
aaataaccga ttaaaggaca ttaatagaca aggtaccaac tcagttagga ctttctctga 89400
tatggccagg tcttccaact ctattgtaaa cgcttacgcg ctagttgctg ctaacgtctt 89460
cgcggttaca gaagcattta gactacttaa cgaagcagct agtgtagaac gtctagaaga 89520
ggtctctgca gttatgtctg gacagttagg tatttccatc atcggagtgg cggaagcatt 89580
gcaagaagct actgacggtg ctgtaagcta ccaagcggcc cttagacagg cagcatctgc 89640
tacagcattt ggcttcgata cccaaaccat ctcagagttt gccacagtag ctagacgtgc 89700
atccatcgtt ctaggtgtgg aaatgactga tgcacttaac cgtgtggtac gtggtgtatc 89760
aaaagcggaa gtggaactat tagacgaact aggtattact gttcgactaa acgaagcgtt 89820
cgctcagtac gccgcagagc ataacattgc agctaacagc ctaaactcct tccagagaca 89880
acaagcgctg gctaatgagg tgatacgtaa atccgagcag aacttaggtg cagtagactc 89940
taggttagaa tctacagctt gggagaagtt cggagctaac gtatctggag ctactaacag 90000
gttattacgc ttaatcgcta ctaatgatat tgttattggt acgttaaacg agattaatgg 90060
tgtgtttgag gctattgata ggtggggccg tgaaggagct gattctttac cagtaattga 90120
tacgcttaaa gaggcaagag tgtctcctgg ctttattgat gacatgctag cactgaacgg 90180
tctactagaa gaacaggaga agctagacaa gaagctacgt aaagctgctc gtgcagccaa 90240
caatgctgcc ccaggacagg tagggcccct agaagatgag gtgcgcgctc ttaaagcaga 90300
atatttactg gcaaccaggg cagtaaatga gtttactaag tctatgggta ctagtaaaga 90360
cactgttaag gctatcgctc ctgagattca gaacttaact accttaagta gaagcctttc 90420
tggtggcatc acaggaatca ttaatgacct tactccagag aagggaacat acgctgggct 90480
agctaatgaa gcaaaggagg ctcagaggtc tgtaaaccta ctaactgagg caggtatctc 90540
ccaaagagag gccttagaga gactaaaaat aacttctgag caattctaca agctagatgc 90600
aattcaggct tacgccaaga ttcaagagaa tcaaaccaaa gagatgcaaa agcaagaaat 90660
gcttgcaagg gagttggacg gacttacaag cttcgatgag gcagtattag tcgcagagca 90720
acagttagaa gctcaaagaa aaatcaatga tgctgccaca gcagtgttag gcatcaaaga 90780
aaaaaccact caaatggttt acgatgagtg ggttctagag cagaaactaa acgcagctcg 90840
ggccaagtct attcagagca gttatgaaaa gctaagaatc cagtccgaat tcgagaatag 90900
aaaccaaagt acattcagag aacctctgga ctctacaaca actctacaaa gagaacttag 90960
cctagagatg tcaatcctag agactatgca gcgccaagag tttatctcca aggctgaagt 91020
cgaggctcag agggagagaa ctaaggagct ggagagacag ttagttatct ctcaagagga 91080
aaactctaga cgtctacgtc aagataacca acagggagtt gacagcacgg tagctacttt 91140
aagtcctgaa atagcggaca tgcagagtca ggtacttgca gcagcggacg cgtgggaaat 91200
ctacggtcaa tcagctaaat cggctgggga cttagcagct gcctctgcaa ctactacagc 91260
atccgcattg aacagtatat ctagtgtaat tcagatggct gggcaggcag cggttggcag 91320
tatagatgct gaaattgcag ccctacagtc taaggaaaac ctaagtgaag cagaagagaa 91380
gaagctaaaa gaactgaaca agaagaagat aaaggagcag gagaaatacg ctaaggccac 91440
tatcctaatc cagacagcgg caggtattgc gtatgcttta ggtagtgctc cacctccact 91500
aagctttata aacgcaggtc ttgtggctgt tgcaggtgct atggcatacc aacaagcttc 91560
taactcagca gcttctcaac tagcagctct tggtaactct actaatgaag gtgctgaccc 91620
aacttctctg aaagtggg 91638

Claims (8)

1. a kind of bacteriophage, it is characterised in that: the DNA sequence dna of bacteriophage is sequence shown in SEQ ID No.1.
2. bacteriophage described in claim 1 is preparing the application in anti-vibrio parahaemolytious preparation.
3. application of the bacteriophage described in claim 1 in preparation treatment vibrio parahaemolytious infection medicine.
4. application of the bacteriophage described in claim 1 in prevention and treatment vibrio parahaemolytious pollution.
5. application according to claim 4, it is characterised in that: vibrio parahaemolytious pollution for aquaculture, transport and Germ contamination during preservation.
6. a kind of antibacterial agent, it is characterised in that: including bacteriophage described in claim 1.
7. a kind of food additives, it is characterised in that: including bacteriophage described in claim 1.
8. a kind of detergent or disinfectant, it is characterised in that: including bacteriophage described in claim 1.
CN201810959650.7A 2018-08-16 2018-08-16 A kind of vibrio parahaemolyticus phage and its application Pending CN109136195A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108699532A (en) * 2015-12-21 2018-10-23 尹特荣生物科技株式会社 Novel vibrio parahaemolyticus phage Vib-PAP-2 and its for inhibit vibrio parahaemolytious be proliferated purposes
CN111172119A (en) * 2020-03-10 2020-05-19 青岛诺安百特生物技术有限公司 Novel vibrio parahaemolyticus phage with wide cracking spectrum, specific primer and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323209A1 (en) * 2012-06-04 2013-12-05 Ctc Bio, Inc. Novel bacteriophage and its use for preventing proliferation of pathogenic bacteria
CN108103031A (en) * 2018-01-10 2018-06-01 浙江省淡水水产研究所 A kind of wide range phage preparation used for aquiculture and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323209A1 (en) * 2012-06-04 2013-12-05 Ctc Bio, Inc. Novel bacteriophage and its use for preventing proliferation of pathogenic bacteria
CN108103031A (en) * 2018-01-10 2018-06-01 浙江省淡水水产研究所 A kind of wide range phage preparation used for aquiculture and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
徐华东: "副溶血弧菌Vp13烈性噬菌体的分离鉴定及生物学特性研究", 《中国优秀硕士论文全文数据库》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108699532A (en) * 2015-12-21 2018-10-23 尹特荣生物科技株式会社 Novel vibrio parahaemolyticus phage Vib-PAP-2 and its for inhibit vibrio parahaemolytious be proliferated purposes
CN111172119A (en) * 2020-03-10 2020-05-19 青岛诺安百特生物技术有限公司 Novel vibrio parahaemolyticus phage with wide cracking spectrum, specific primer and application thereof

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