CN112656905A

CN112656905A - Composition for treating deficiency of qi and blood and preparation method and application thereof

Info

Publication number: CN112656905A
Application number: CN202110139024.5A
Authority: CN
Inventors: 程宗琦; 张春歌; 陈琳; 朱建国; 毛叶勤; 缪丽燕; 闫兆威; 吴憩; 姚鑫; 刘林生
Original assignee: First Affiliated Hospital of Suzhou University
Current assignee: First Affiliated Hospital of Suzhou University
Priority date: 2021-02-01
Filing date: 2021-02-01
Publication date: 2021-04-16

Abstract

The invention provides a composition for treating deficiency of qi and blood, a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. A composition for treating deficiency of qi and blood comprises the following raw materials: radix astragali, Saviae Miltiorrhizae radix, radix Codonopsis, fructus Lycii, herba et Gemma Agrimoniae, caulis Spatholobi, radix rehmanniae, fructus Hordei Germinatus preparata, Atractylodis rhizoma preparata, radix Angelicae sinensis, endothelium corneum Gigeriae Galli preparata, fructus Ligustri Lucidi preparata, herba Epimedii, Coptidis rhizoma, colla Cornus Cervi, colla Plastri Testudinis and Notoginseng radix powder; the composition for treating deficiency of both qi and blood adopts various traditional Chinese medicinal materials with mild medicinal properties to carry out compatibility, not only can tonify yang qi and essence and blood, but also can treat anemia symptoms such as aplastic anemia and the like, and bone marrow suppression caused by thrombopenia, leukopenia and tumor radiotherapy and chemotherapy and treat diseases causing deficiency of both qi and blood. In addition, the invention also provides a preparation method of the composition for treating deficiency of both qi and blood, and the preparation method of the composition for treating deficiency of both qi and blood not only improves the medicinal properties of the composition for treating deficiency of both qi and blood, but also has simple process, easy operation and convenient use.

Description

Composition for treating deficiency of qi and blood and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and particularly relates to a composition for treating deficiency of qi and blood, and a preparation method and application thereof.

Background

In traditional Chinese medicine, the deficiency of qi and blood refers to qi deficiency and blood deficiency, and the deficiency of qi and blood can cause hypofunction of viscera, which causes sub-health state of the organism and even premature senility. Qi deficiency means the hypofunction of the zang-fu organs and poor resistance to disease, manifested as aversion to cold, cold limbs, spontaneous perspiration, dizziness, tinnitus, listlessness, fatigue, weakness, palpitation, shortness of breath and hypoevolutism. Blood deficiency can lead to lusterless sallow complexion, dry skin, withered hair, dry nails, dim eyesight, numb hands and feet, insomnia, dreaminess, amnesia, palpitation and absentmindedness. Deficiency of both qi and blood pertains to both qi and blood disorders. Qi and blood deficiency can cause malnutrition of the body, with the common symptoms of lassitude, shortness of breath, laziness in speaking, pale or sallow complexion, dizziness, pale lips and nails, palpitation, insomnia, pale tongue and weak pulse.

Qi deficiency and blood deficiency may occur alone or in combination. The deficiency of qi and blood is usually caused by insufficient rest time and over-fatigue of a human body, the condition is better recovered, and the deficiency of qi and blood caused by the deficiency of hematopoietic function or the reduction of blood cells caused by own diseases, chemotherapy, radiotherapy and the like can be improved by taking the common traditional Chinese medicine health care products for replenishing qi and blood on the market.

Most of the traditional Chinese medicines capable of replenishing qi and blood on the market are health-care products, only can improve qi and blood loss caused by insufficient rest time or excessive fatigue of a human body, and have no policy for restraining qi and blood loss caused by hemopoietic function loss or blood cell reduction caused by self diseases, radiotherapy, chemotherapy and the like.

Disclosure of Invention

The invention aims to provide a composition for treating deficiency of qi and blood, which adopts a plurality of traditional Chinese medicinal materials with mild medicinal properties for compatibility, can nourish yang, qi and essence and blood, can treat anemia symptoms such as aplastic anemia and the like, and can treat bone marrow suppression caused by thrombopenia, leukopenia and tumor radiotherapy and chemotherapy and diseases causing deficiency of qi and blood, and fundamentally treat the deficiency of qi and blood.

The invention also aims to provide application of the composition for treating deficiency of both qi and blood in preparing medicaments for treating anemia and thrombocytopenia and leukopenia and recovering hemogram.

Another object of the present invention is to provide a method for preparing a composition for treating deficiency of both qi and blood, which improves the medicinal properties of the composition for treating deficiency of both qi and blood by concentrating and concentrating the extract, and has a simple process, easy operation, and convenient use.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

On one hand, the embodiment of the application provides a composition for treating deficiency of qi and blood, which is mainly prepared from the following raw materials in parts by weight: 75-85 parts of astragalus membranaceus, 25-35 parts of salvia miltiorrhiza, 55-65 parts of codonopsis pilosula, 40-50 parts of wolfberry fruit, 85-95 parts of hairyvein agrimony, 85-95 parts of caulis spatholobi, 45-55 parts of radix rehmanniae recen, 25-35 parts of roasted malt, 35-45 parts of roasted bighead atractylodes rhizome, 55-65 parts of angelica sinensis, 15-25 parts of roasted chicken's gizzard-membrane, 40-50 parts of wine glossy privet fruit, 15-25 parts of epimedium herb, 10-20 parts of coptis chinensis, 25-35 parts of deerhorn glue, 25-35 parts of tortoise-shell glue and 5-15 parts of pseudo.

In another aspect, the present application provides the use of a composition for treating deficiency of both qi and blood in the manufacture of a medicament for treating anemia and platelet, leukopenia and restoring hemogram.

In another aspect, the present application provides a method for preparing a composition for treating deficiency of qi and blood, comprising the following steps: soaking the other raw materials except the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi in water, decocting to obtain a decoction, concentrating the decoction to obtain a concentrated solution, and adding the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi into the concentrated solution to obtain paste, thus obtaining the composition for treating deficiency of qi and blood.

The preparation method of the composition for treating deficiency of both qi and blood adopts the steps of decoction, concentration and paste collection, so that the volume of the composition for treating deficiency of both qi and blood can be reduced, the composition is convenient to take, and the medicinal properties of the composition are improved; and the preparation method is simple and convenient to operate, is easy to operate, and is convenient for operators to operate.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

the composition for treating deficiency of qi and blood provided by the invention adopts a plurality of medicinal materials for replenishing blood and tonifying qi, and the medicinal materials with cold and warm natures are mutually harmonized and restricted according to a certain proportion, so that the effect of harmonizing yin and yang is achieved; in addition, the medicinal materials are combined according to a reasonable monarch, minister, assistant and guide relationship, so the composition for treating deficiency of qi and blood provided by the invention has better curative effect on treating symptoms causing deficiency of qi and blood, such as anemia symptoms of aplastic anemia and the like, and blood cell reduction caused by radiotherapy and chemotherapy, and the composition for treating deficiency of qi and blood can also clear heat and dry dampness, nourish yin and tonify yang, strengthen stomach and promote digestion, arrest seminal emission and stop enuresis, and regulate the physical condition from multiple aspects.

Wherein the monarch drugs comprise radix astragali, colla Cornus Cervi and colla Plastri Testudinis, the radix astragali is mainly selected from root of radix astragali, has sweet taste and mild nature, and has effects of invigorating qi and promoting blood production, invigorating spleen and lung channel, and is mainly used for treating symptoms such as qi deficiency and debilitation; the deer-horn glue is prepared by adding crystal sugar, yellow wine, soybean oil and other auxiliary materials into deer-horn, has light smell and slightly sweet taste, can warm liver and tonify kidney, replenish essence and nourish blood, and is mainly used for treating symptoms of blood deficiency, dizziness, physical weakness, hypodynamia and the like; the tortoise-shell glue is prepared by adding yellow wine and other auxiliary materials into tortoise shells, has light smell and taste, can nourish yin and blood, and is mainly used for treating yin deficiency hectic fever, blood deficiency chlorosis and other symptoms. The three medicinal materials are compatible by adopting a proper proportion, can promote qi circulation and invigorate qi, nourish kidney essence, nourish liver blood, tonify congenital and acquired, and harmonize qi, blood, yin and yang, and the three materials act together to supplement each other to be monarch drugs.

The ministerial drugs comprise radix codonopsitis, fried rhizoma atractylodis macrocephalae and angelica, the radix codonopsitis is mainly selected from dried roots of the radix codonopsitis and is sweet in taste and mild in nature, and can tonify middle-jiao and Qi, enrich blood and promote fluid production and mainly treat symptoms such as tiredness and weakness and deficiency of both qi and blood; the Atractylodis rhizoma is perennial herb of Atractylodes of Compositae, has sweet taste and warm nature, can invigorate qi and invigorate spleen, eliminate dampness and promote diuresis, and is mainly used for treating symptoms such as weakness of spleen and stomach and anorexia; the root of the angelica is selected as a medicine, has slightly sweet taste and warm nature, can tonify qi and blood, invigorate blood circulation and moisten intestines, and is mainly used for treating symptoms such as blood deficiency, fever, excessive blood loss and the like. The compatibility of the three medicinal materials and the astragalus root has the effects of supplementing blood and tonifying qi, wherein the compatibility of the angelica and the astragalus root has the theory of angelica blood-enriching soup, has the effect of strengthening and nourishing essence and blood by being matched with tortoise shell glue and antler glue, and can activate blood while enriching blood without blood stagnation.

The adjuvant drugs comprise fructus Lycii, fructus Ligustri Lucidi processed with wine, Saviae Miltiorrhizae radix, caulis Spatholobi, endothelium corneum Gigeriae Galli preparata, fructus Hordei Germinatus preparata, herba Epimedii, herba et Gemma Agrimoniae, Coptidis rhizoma, radix rehmanniae and Notoginseng radix powder, and fructus Lycii is mature fruit of fructus Lycii, has sweet taste and mild property, can nourish liver and kidney, and benefit essence and improve eyesight, and is used for treating deficiency of essence and blood and deficiency of liver and kidney; the wine glossy privet fruit is prepared from ripe glossy privet fruits, is sweet and bitter in taste and mild in nature, can nourish yin, prolong life, and tonify liver and kidney, and is mainly used for treating symptoms such as light headedness and the like caused by qi and blood deficiency; the root and the stem of the salvia miltiorrhiza are mainly selected as the raw materials, the salvia miltiorrhiza is bitter in taste and slightly cold in nature, can tonify qi and activate blood, and is mainly used for treating symptoms such as chest stuffiness, pains, vexation, insomnia and the like; the caulis spatholobi is mainly used as a medicine for rattan stems, is bitter and sweet in taste and warm in nature, can promote blood circulation and enrich the blood, and is mainly used for treating blood deficiency and chlorosis and other symptoms; the fried endothelium corneum Gigeriae Galli is prepared by frying fresh endothelium corneum Gigeriae Galli, has sweet taste and mild property, can invigorate stomach, promote digestion, astringe essence and relieve enuresis, and is mainly used for treating indigestion, enuresis and spermatorrhea; the fried malt is prepared by frying malt, can promote qi circulation, promote digestion, invigorate spleen and stimulate appetite, and is mainly used for treating symptoms such as indigestion, spleen deficiency and anorexia; the epimedium is mainly selected from the leaves of the epimedium as a medicine, has pungent and sweet taste and warm nature, can tonify kidney yang, and is mainly used for treating symptoms such as kidney yang deficiency and the like; the hairyvein agrimony is mainly selected from stems and leaves thereof to be used as a medicine, has bitter and astringent taste and mild property, can stop bleeding and tonify deficiency, and is mainly used for treating symptoms such as metrorrhagia, metrostaxis and bleeding; the coptis is mainly selected from dried rhizome parts of the coptis as a medicine, is bitter in taste and cold in nature, can clear heat and dry dampness, and is mainly used for treating symptoms such as blood heat hematemesis and epistaxis; dried root tubers of the dried rehmannia root are mainly selected as the raw materials, are sweet in taste and slightly warm in nature, can enrich blood, nourish yin, replenish vital essence and fill marrow, and are mainly used for treating blood deficiency, chlorosis, liver and kidney yin deficiency and other symptoms; the notoginseng is mainly selected from dried root thereof as a medicine, has sweet and slightly bitter taste and warm nature, can remove blood stasis and stop bleeding, and activate blood and relieve pain, and is mainly used for treating symptoms such as traumatic hemorrhage, hematochezia and the like. The medlar and the wine glossy privet fruit can cooperate with the tortoise-shell glue, the antler glue and the angelica to tonify the essence and blood of the liver and kidney, the epimedium and the antler glue can warm the kidney and strengthen yang, and have the effect of reinforcing the yang and invigorating the yin by being matched with various medicinal materials for nourishing yin and blood in the adjuvant drugs, and the coptis chinensis is cold in nature, can harmonize the property of the warm drugs and has the efficacy of cooperating with hemostasis.

In addition, the preparation method of the composition for treating deficiency of both qi and blood provided by the invention has the advantages of simple operation steps, easiness in use, convenience for operators to operate and reduction in operation difficulty.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a graph showing the tendency of weight change of mice in each group in test example 1 of the present invention, wherein C is a normal group, M is a model control group, and D is an administration group;

FIG. 2 is a graph showing the conventional tendency of peripheral blood changes of mice in each group in test example 1 of the present invention, wherein C is a normal group, M is a model control group, and D is an administration group;

FIG. 3 is a graph showing the trend of change in thymus index and spleen index of each group of mice in test example 1 of the present invention, wherein C is a normal group, M is a model control group, and D is an administered group;

FIG. 4 is a pathological morphology pattern observed under a 400-fold microscope after HE staining of bone marrow tissue of a normal group of mice in experimental example 1 of the present invention;

FIG. 5 is a pathological morphology pattern observed under a 400-fold microscope after HE staining of bone marrow tissue of a mouse in a model control group in Experimental example 1 of the present invention;

FIG. 6 is a pathological morphology pattern observed under a 400-fold microscope after HE staining of bone marrow tissue of mice in the test example 1 of the present invention.

Icon: 1-megakaryocytes; 2-lobular nuclear granulocytes; 3-the blood sinuses.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

On one hand, the embodiment of the application provides a composition for treating deficiency of qi and blood, which is mainly prepared from the following raw materials in parts by weight: 75-85 parts of astragalus membranaceus, 25-35 parts of salvia miltiorrhiza, 55-65 parts of codonopsis pilosula, 40-50 parts of wolfberry fruit, 85-95 parts of hairyvein agrimony, 85-95 parts of caulis spatholobi, 45-55 parts of radix rehmanniae recen, 25-35 parts of roasted malt, 35-45 parts of roasted bighead atractylodes rhizome, 55-65 parts of angelica sinensis, 15-25 parts of roasted chicken's gizzard-membrane, 40-50 parts of wine glossy privet fruit, 15-25 parts of epimedium herb, 10-20 parts of coptis chinensis, 25-35 parts of deerhorn glue, 25-35 parts of tortoise-shell glue and 5-15 parts of pseudo. Wherein the monarch drugs comprise radix astragali, colla Cornus Cervi and colla Plastri Testudinis, the radix astragali is mainly selected from root of radix astragali, has sweet taste and mild nature, and has effects of invigorating qi and promoting blood production, invigorating spleen and lung channel, and is mainly used for treating symptoms such as qi deficiency and debilitation; the deer-horn glue is prepared by adding crystal sugar, yellow wine, soybean oil and other auxiliary materials into deer-horn, has light smell and slightly sweet taste, can warm liver and tonify kidney, replenish essence and nourish blood, and is mainly used for treating symptoms of blood deficiency, dizziness, physical weakness, hypodynamia and the like; the tortoise-shell glue is prepared by adding yellow wine and other auxiliary materials into tortoise shells, has light smell and taste, can nourish yin and blood, and is mainly used for treating yin deficiency hectic fever, blood deficiency chlorosis and other symptoms. The three medicinal materials are compatible by adopting a proper proportion, can promote qi circulation and invigorate qi, nourish kidney essence, nourish liver blood, tonify congenital and acquired, and harmonize qi, blood, yin and yang, and the three materials act together to supplement each other to be monarch drugs.

The adjuvant drugs comprise fructus Lycii, fructus Ligustri Lucidi processed with wine, Saviae Miltiorrhizae radix, caulis Spatholobi, endothelium corneum Gigeriae Galli preparata, fructus Hordei Germinatus preparata, herba Epimedii, herba et Gemma Agrimoniae, Coptidis rhizoma, radix rehmanniae and Notoginseng radix powder, and fructus Lycii is mature fruit of fructus Lycii, has sweet taste and mild property, can nourish liver and kidney, and benefit essence and improve eyesight, and is used for treating deficiency of essence and blood and deficiency of liver and kidney; the wine glossy privet fruit is mature fruit of glossy privet, is sweet and bitter in taste and neutral in nature, can nourish yin, prolong life, and tonify liver and kidney, and is mainly used for treating symptoms such as dizziness and the like caused by deficiency of qi and blood; the root and the stem of the salvia miltiorrhiza are mainly selected as the raw materials, the salvia miltiorrhiza is bitter in taste and slightly cold in nature, can tonify qi and activate blood, and is mainly used for treating symptoms such as chest stuffiness, pains, vexation, insomnia and the like; the caulis spatholobi is mainly used as a medicine for rattan stems, is bitter and sweet in taste and warm in nature, can promote blood circulation and enrich the blood, and is mainly used for treating blood deficiency and chlorosis and other symptoms; the fried endothelium corneum Gigeriae Galli is prepared by frying fresh endothelium corneum Gigeriae Galli, has sweet taste and mild property, can invigorate stomach, promote digestion, astringe essence and relieve enuresis, and is mainly used for treating indigestion, enuresis and spermatorrhea; the fried malt is prepared by frying malt, can promote qi circulation, promote digestion, invigorate spleen and stimulate appetite, and is mainly used for treating symptoms such as indigestion, spleen deficiency and anorexia; the epimedium is mainly selected from the leaves of the epimedium as a medicine, has pungent and sweet taste and warm nature, can tonify kidney yang, and is mainly used for treating symptoms such as kidney yang deficiency and the like; the hairyvein agrimony is mainly selected from stems and leaves thereof to be used as a medicine, has bitter and astringent taste and mild property, can stop bleeding and tonify deficiency, and is mainly used for treating symptoms such as metrorrhagia, metrostaxis and bleeding; the coptis is mainly selected from dried rhizome parts of the coptis as a medicine, is bitter in taste and cold in nature, can clear heat and dry dampness, and is mainly used for treating symptoms such as blood heat hematemesis and epistaxis; dried root tubers of the dried rehmannia root are mainly selected as the raw materials, are sweet in taste and slightly warm in nature, can enrich blood, nourish yin, replenish vital essence and fill marrow, and are mainly used for treating blood deficiency, chlorosis, liver and kidney yin deficiency and other symptoms; the notoginseng is mainly selected from dried root thereof as a medicine, has sweet and slightly bitter taste and warm nature, can remove blood stasis and stop bleeding, and activate blood and relieve pain, and is mainly used for treating symptoms such as traumatic hemorrhage, hematochezia and the like. The medlar and the wine glossy privet fruit can cooperate with the tortoise-shell glue, the antler glue and the angelica to tonify the essence and blood of the liver and kidney, the epimedium and the antler glue can warm the kidney and strengthen yang, and have the effect of reinforcing the yang and invigorating the yin by being matched with various medicinal materials for nourishing yin and blood in the adjuvant drugs, and the coptis chinensis is cold in nature, can harmonize the property of the warm drugs and has the efficacy of cooperating with hemostasis.

In some embodiments of the present invention, the composition for treating deficiency of qi and blood is mainly prepared from the following raw materials in parts by weight: 80 parts of astragalus, 30 parts of salvia miltiorrhiza, 60 parts of codonopsis pilosula, 45 parts of wolfberry, 90 parts of hairyvein agrimony, 90 parts of suberect spatholobus stem, 50 parts of radix rehmanniae recen, 30 parts of fried malt, 40 parts of fried bighead atractylodes rhizome, 60 parts of Chinese angelica, 20 parts of fried chicken gizzard membrane, 45 parts of wine glossy privet fruit, 20 parts of epimedium herb, 15 parts of coptis chinensis, 30 parts of deerhorn glue, 30 parts of tortoise-shell glue and 10 parts of pseudo-ginseng powder.

The above embodiments are the optimal formula of the composition for treating deficiency of qi and blood, and not only have significant efficacy in treating the disease causing deficiency of qi and blood, but also can clear heat and dry dampness, nourish yin and tonify yang, strengthen stomach and promote digestion, and arrest seminal emission and arrest spermatorrhea.

In another aspect, the present application provides a method for preparing a composition for treating deficiency of qi and blood, comprising the following steps: soaking the other raw materials except the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi in water, decocting to obtain a decoction, concentrating the decoction to obtain a concentrated solution, and adding the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi into the concentrated solution to obtain paste, thus obtaining the composition for treating deficiency of qi and blood. The pseudo-ginseng powder, the antler glue and the tortoise-shell glue are added after decoction and concentration, so that the loss of medicine property caused by pot pasting of the antler glue and the tortoise-shell glue during decoction is avoided, and the loss of medicine property caused by loss and waste of the pseudo-ginseng powder during the decoction process is easily caused, so that the pseudo-ginseng powder is directly added after concentration; soaking in water for 0.5 hr before decocting.

In some embodiments of the present invention, the step of sterilizing before soaking further comprises a step of sterilizing, wherein the step of sterilizing is to expose the raw material to ultraviolet light of 255-265nm for 10-20 min. The sterilization can adopt ultraviolet sterilization, high-pressure steam sterilization and other modes, wherein the ultraviolet sterilization is preferred, the ultraviolet sterilization is not only simple and easy to operate, but also does not damage the property of the medicinal materials.

In some embodiments of the present invention, the tortoise shell glue and the antler glue are further processed before being added into the concentrated solution, and the processing is performed by processing the tortoise shell glue and the antler glue to be crisp by a sand scalding method. The processing method can adopt stir-frying and sand-scalding, wherein sand-scalding method is preferred, and the method can not only improve the taste of the medicinal materials, but also can not damage the property of the medicinal materials.

In some embodiments of the invention, the decoction is divided into two times, the first decoction is carried out for 40-50min, and a first decoction is obtained after filtration; adding water again for second decoction, wherein the time for the second decoction is 40-50min, filtering to obtain a second decoction, and mixing the first decoction and the second decoction. Beneficial components in the medicinal materials are completely extracted by twice decoction, and water is added in twice, so that the phenomenon that part of the beneficial components are lost along with water evaporation due to excessive water addition caused by adding water only in the first decoction is avoided.

In some embodiments of the present invention, the amount of water added in the first decoction and the amount of water added in the second decoction are respectively 12 times of the weight of the added medicinal materials, and the first decoction and the second decoction are both boiled with strong fire and then slowly decocted with slow fire. Water is added in the two times, and the water adding amount is the same, so that the beneficial components in the medicinal materials can be extracted to the maximum degree when the medicinal materials are decocted for two times. And the medicines are decocted by slow fire, so that the damage to the property of the medicines caused by excessive fire is avoided.

In some embodiments of the invention, the concentration is controlled such that the relative density of the concentrate is 1.02 to 1.1 at 60 ℃. The density of the concentrated solution is ensured to be in a reasonable interval, so that the content of beneficial components in the concentrated solution per gram of the concentrated solution is ensured.

In some embodiments of the invention, the relative density of the composition for controlling the deficiency of qi and blood at the time of harvesting the paste is 1.1 to 1.2 at 60 ℃. The relative density of the composition for treating deficiency of qi and blood is ensured in a reasonable interval during paste collection, so that the content of beneficial components in each gram of the composition for treating deficiency of qi and blood is ensured.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The present embodiment aims to provide a composition for treating deficiency of qi and blood.

The composition adopts the following raw materials: 75g of astragalus, 25g of salvia miltiorrhiza, 55g of codonopsis pilosula, 40g of wolfberry fruit, 85g of hairyvein agrimony, 85g of suberect spatholobus stem, 45g of radix rehmanniae recen, 25g of fried malt, 35g of fried bighead atractylodes rhizome, 55g of Chinese angelica, 15g of fried chicken gizzard membrane, 40g of wine-treated glossy privet fruit, 15g of epimedium herb, 10g of coptis chinensis, 25g of deerhorn glue, 25g of tortoise-shell glue and 5g of pseudo-ginseng.

The composition is prepared by the following steps: irradiating the medicinal materials for 10 minutes by using 255nm ultraviolet light, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 7260mL of water, soaking for 0.5h, starting first decoction, boiling by using strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 40min to obtain a first decoction, adding 7260mL of water for second decoction, adjusting the fire to be strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 40min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to a relative density of 1.02(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by using a sand boiling method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and obtaining paste to a relative density of 1.1(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Example 2

The composition adopts the following raw materials: 85g of astragalus, 35g of salvia miltiorrhiza, 65g of codonopsis pilosula, 50g of wolfberry, 95g of hairyvein agrimony, 95g of suberect spatholobus stem, 55g of radix rehmanniae recen, 35g of fried malt, 45g of fried bighead atractylodes rhizome, 65g of Chinese angelica, 25g of fried chicken gizzard membrane, 50g of wine-treated glossy privet fruit, 25g of epimedium herb, 20g of coptis chinensis, 35g of deerhorn glue, 35g of tortoise-shell glue and 15g of pseudo-ginseng powder.

The composition is prepared by the following steps: irradiating the medicinal materials for 20 minutes by using 265nm ultraviolet light, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 8940mL of water, soaking for 0.5h, starting first decoction, boiling by using strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 50min to obtain a first decoction, adding 8940mL of water for second decoction, adjusting the fire to be strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 50min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to a relative density of 1.1(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by using a sand-boiling method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and obtaining paste to a relative density of 1.2(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Example 3

The composition adopts the following raw materials: 80g of astragalus, 30g of salvia miltiorrhiza, 60g of codonopsis pilosula, 45g of wolfberry, 90g of hairyvein agrimony, 90g of suberect spatholobus stem, 50g of radix rehmanniae recen, 30g of fried malt, 40g of fried bighead atractylodes rhizome, 60g of Chinese angelica, 20g of fried chicken gizzard membrane, 45g of wine glossy privet fruit, 20g of epimedium herb, 15g of coptis chinensis, 30g of deerhorn glue, 30g of tortoise-shell glue and 10g of pseudo-ginseng powder.

The composition is prepared by the following steps: irradiating the medicinal materials for 15 minutes by using ultraviolet light with the wavelength of 260nm, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 8100mL of water, soaking for 0.5h, starting first decoction, boiling by using strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 45min to obtain a first decoction, adding 8100mL of water for second decoction, adjusting the fire to be strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 45min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to the relative density of 1.06(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by using a sand ironing method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and obtaining paste to the relative density of 1.15(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Example 4

The composition adopts the following raw materials: 75g of astragalus, 32g of salvia miltiorrhiza, 60g of codonopsis pilosula, 40g of wolfberry, 85g of hairyvein agrimony, 86g of suberect spatholobus stem, 46g of radix rehmanniae recen, 32g of fried malt, 35g of fried bighead atractylodes rhizome, 54g of Chinese angelica, 15g of fried chicken's gizzard-membrane, 40g of wine-treated glossy privet fruit, 15g of epimedium herb, 17g of coptis chinensis, 24g of deerhorn glue, 31g of tortoise-shell glue and 5g of pseudo.

The composition is prepared by the following steps: irradiating the medicinal materials for 13 minutes by using 258nm ultraviolet light, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 7584mL of water, soaking for 0.5h, then starting first decoction, boiling by using strong fire, modulating the strong fire to be slow fire after boiling until the materials are slightly boiled, decocting for 44min to obtain a first decoction, then adding 7584mL of water for second decoction, modulating the fire to be strong fire, modulating the strong fire to be slow fire after boiling until the materials are slightly boiled, decocting for 44min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to a relative density of 1.05(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by using a sand-boiling method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and obtaining paste until the relative density of 1.14(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Example 5

The composition adopts the following raw materials: 77g of astragalus, 33g of salvia miltiorrhiza, 63g of codonopsis pilosula, 42g of wolfberry fruit, 87g of hairyvein agrimony, 87g of suberect spatholobus stem, 47g of radix rehmanniae recen, 33g of fried malt, 37g of fried bighead atractylodes rhizome, 57g of Chinese angelica, 17g of fried chicken's gizzard-membrane, 42g of wine-treated glossy privet fruit, 17g of epimedium herb, 18g of coptis chinensis, 27g of deerhorn glue, 33g of tortoise-shell glue and 7g of.

The composition is prepared by the following steps: irradiating the medicinal materials for 18 minutes by using 262nm ultraviolet light, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 7884mL of water, soaking for 0.5h, starting first decoction, boiling by using strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 41min to obtain a first decoction, adding 7884mL of water for second decoction, adjusting the fire to be strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 41min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to a relative density of 1.03(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by using a sand-boiling method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and obtaining paste to a relative density of 1.16(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Example 6

The composition adopts the following raw materials: 78g of astragalus, 34g of salvia miltiorrhiza, 64g of codonopsis pilosula, 43g of wolfberry fruit, 88g of hairyvein agrimony, 88g of suberect spatholobus stem, 48g of radix rehmanniae recen, 34g of fried malt, 38g of fried bighead atractylodes rhizome, 58g of Chinese angelica, 18g of fried chicken's gizzard-membrane, 43g of wine-treated glossy privet fruit, 18g of epimedium herb, 19g of coptis chinensis, 28g of deerhorn glue, 34g of tortoise-shell glue and 8g of.

The composition is prepared by the following steps: irradiating the medicinal materials with 259nm ultraviolet light for 17 minutes, adding the medicinal materials except the pseudo-ginseng powder, the antler glue and the tortoise-shell glue into a boiling pot, adding 8052mL of water, soaking for 0.5h, then starting first decoction, boiling with strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 46min to obtain a first decoction, adding 8052mL of water for second decoction, adjusting the fire to be strong fire, adjusting the strong fire to be slow fire to slightly boil after boiling, decocting for 46min to obtain a second decoction, combining the first decoction and the second decoction to obtain a decoction, concentrating the decoction to a relative density of 1.08(60 ℃) to obtain a concentrated solution, treating the tortoise-shell glue and the antler glue to be crisp by a sand-boiling method, adding the pseudo-ginseng powder, the tortoise-shell glue and the antler glue into the concentrated solution, and collecting paste to a relative density of 1.17(60 ℃), thus obtaining the composition for treating deficiency of qi and blood.

Test example 1

Preparation of experimental six-week-old ICR mice, SPF grade, male, purchased from shanghai sipel-bikeka experimental animals limited, experimental animals license number: SCXK (Shanghai) 2018 and 0006. The mice are raised in the environment with the temperature of 20 +/-2 ℃ and the humidity of 60 +/-2% RH for one week to adapt to the environment, and the mice are fed with water freely during the raising process.

Prepared were acetylphenylhydrazine (APH, 98% pure, available from Shanghai Aladdin Biochemical technology Co., Ltd.), cyclophosphamide (CTX, available from Jiangsu Henry pharmaceuticals Co., Ltd.), the composition for treating deficiency of both qi and blood prepared in example 1-4, physiological saline, paraformaldehyde and disodium ethylenediaminetetraacetate (EDTA-2Na, national group chemical reagent Co., Ltd., batch No.: T20120311).

Preparing a full-automatic blood analyzer (manufacturer: HORIBA Medical; instrument model: ABX Pentra XL 80); an ice maker (manufacturer: PANASONIC; instrument model: SIM-F140AY 65-PC); centrifuge (manufacturer: Beijing Boya Innovation science and technology development Co., Ltd.; model: Micofuge 22R Centrifuge).

The prepared ICR mice were randomly divided into three groups by body weight,10 normal groups, 10 model control groups, and 10 administration groups, wherein the mice of the administration group were gavaged in example 1; the dose was 0.4 mL-20 g^-1·d^-113d, the normal group and the model control group were administered with the same amount of drinking water.

Wherein the model control group and administration group mice are injected with APH-physiological saline solution subcutaneously at 7 th and 10 th d, respectively, and the dose of 7 th d is 20 mg-kg^-1The dose of 10d is 10 mg/kg ^-110 th, 11 th, 12 th and 13 th intraperitoneal injection of CTX-physiological saline solution, the dosage of each time is 20mg kg^-1(ii) a Wherein the 10 th day is firstly injected with APH-normal saline solution subcutaneously, and then is injected with CTX-normal saline solution intraperitoneally after 2 hours.

Mice in each group were weighed every other day, body weight recorded and a curve was drawn. Each group of mice was administered with the last day of the experimental period or 30min after an equivalent amount of drinking water was administered, and peripheral blood routine (mainly including peripheral blood leukocyte count (WBC/10)⁹·L^-1) Red blood cell count (RBC/10)¹²·L^-1) Hemoglobin (HGB/g.L)^-1) Hematocrit (HCT/%), reticulocyte count (RTC/10)¹²·L^-1) Detection of (3). Then, the mice are killed after cervical vertebra is removed, thymus and spleen are taken for weighing, TI (thymus index, TI) and SI (spleen index, SI) are calculated according to a formula, TI is thymus weight (mg)/mouse weight (g), SI is spleen weight (mg)/mouse weight (g), and pathology examination is carried out on thighbone of the mice (test data are counted by Descriptives in SPSS 16.0 software, and test results are expressed by x +/-s; and compared with a model group by a Dunnett method in variance analysis in the software); wherein the change in body weight of the mouse is shown in FIG. 1, the conventional change in peripheral blood of the mouse is shown in FIG. 2, and the change in thymus index and spleen index of the mouse is shown in FIG. 3, wherein ". x" indicates P < 0.05 and ". x" indicates P < 0.01 in comparison between the normal group and the model control group. In the model control group and the administration group, "#" indicates that P < 0.05 and "##" indicates that P < 0.01.

According to test example 1, the body weight of the normal group mice showed a slow growth tendency, the body weight of the model group mice showed a slow growth tendency before the model formation, the body weight of the model group mice showed a downward tendency after the model formation, the body weight of the administration group showed a fast growth on days 1 to 7, and the body weight showed a slow growth from the model formation. After molding, WBC, RBC, HGB, HCT and RTC are all reduced, and have significant difference (P is less than 0.05, P is less than 0.01), which indicates that molding is successful; after administration, all the other indexes except WBC were significantly increased (P < 0.05, P < 0.01), suggesting that the composition for treating deficiency of both qi and blood prepared in example 1 had significant effects on erythroid cells. After the modeling, the thymus index is obviously reduced, the spleen index is obviously increased, which indicates that the acetylphenylhydrazine and the cyclophosphamide can cause the damage of the thymus and the spleen, mainly show that the thymus is atrophied and the spleen is enlarged (P is less than 0.05 and P is less than 0.01), and the success of the modeling is prompted, and the enlargement of the spleen can be obviously improved after the administration (P is less than 0.05).

The pathological morphology of bone marrow tissue of each group of mice was observed under a microscope, and the results are shown in fig. 4-6, wherein fig. 4 is a pathological morphology chart of bone marrow tissue of normal group of mice, in which hematopoietic cells are abundant, densely arranged, and distributed between the

blood sinuses

3 and 3, the proportion of three systems is normal, and the number of bone marrow nucleated cells and megakaryocytes 1 is large. Wherein FIG. 5 is a pathological morphology of bone marrow tissue of mice in the model control group, compared with FIG. 4, most bone marrow tissue shows necrosis, edema and sinus dilation, the number of bone marrow cells is significantly reduced, and the granuloma is mainly the mid-late juvenile granulocytes and the lobulated nuclear granulocytes 2. Wherein FIG. 6 is a pathological morphology of bone marrow tissue of mice in the administration group, and compared with FIG. 5, the bone marrow tissue was repaired to a certain extent, the expansion of blood sinuses was not significant, the number of nucleated cells and megakaryocytes 1 in bone marrow was significantly increased, and the three-line ratio was approximately normal. Therefore, it can be seen that the composition for treating deficiency of both qi and blood is effective in improving the hematopoietic function of mouse bone marrow.

Test example 2

The method comprises the following steps of (1) drawing up a patient who is diagnosed as malignant tumor through pathology or histocytology according to inclusion criteria ((1) drawing up paclitaxel and platinum chemotherapy according to a medical oncology manual), (2) the patient is 18-80 years old and is expected to have a survival period of more than 3 months), (3) the patient is generally in a good condition, the Ka's functional status score is more than or equal to 60 points, (4) the patient has no obvious chemotherapy taboo and is subjected to hemogram before chemotherapy and basic normal liver and kidney functions and exclusion criteria ((1) important functional damages of liver, kidney, blood system and the like occur, (2) the patient has a history of previous chemotherapy, (3) other treatment means such as combined targeted treatment and immunotherapy during chemotherapy, (4) the patient has bone marrow metastasis or invasion of tumor, (5) the pregnant or lactating woman, (6) the patient with intelligence and mental disorders and language expression disorders and the like, (7) the patient who has good medicine discontinuance and has poor medicine use from the patient, and 60 patients who have poor medicine are selected from the first oncology hospital affiliated to Suzhou university during The volunteers of (1) were used as study cases. The 60 persons were averagely divided into experimental groups of 24 males and 6 females with a mean age of 61.37 ± 9.27 years, containing disease species: 12 cases of esophageal cancer, 9 cases of lung cancer, 5 cases of ovarian cancer, 2 cases of laryngeal cancer, 1 case of gastric cancer and 1 case of thymus gland cancer. Of these, 19 males and 11 females in the control group, with a mean age of 63.57 ± 9.08 years, contained disease species: lung cancer 20 cases, ovarian cancer 9 cases, and esophageal cancer 1 case.

The administration scheme is as follows: the first chemotherapy was performed on these 60 volunteers according to the following protocol: the paclitaxel combined platinum (cisplatin/carboplatin/nedaplatin) is treated by standard dose chemotherapy according to body weight and body surface area, and the observation period is from the beginning of the first chemotherapy to the time before the next chemotherapy. The composition for treating deficiency of qi and blood prepared in example 3 was orally administered to the experimental group 2 times a day with 20mL of warm water each time from the first day of chemotherapy. The control group is administered with warm boiled water 2 times daily. The clinical efficacy and safety of the composition for treating deficiency of both qi and blood prepared in example 3 on bone marrow suppression caused by chemotherapy were evaluated by comparing the relevant indexes before chemotherapy of two groups of patients and after chemotherapy of this period until before chemotherapy of the next period.

Incidence and extent of myelosuppression: referring to the graduation standard of acute and subacute toxic and side effects of chemotherapeutic drugs published by the world health organization (as shown in table 1), the blood routine of the experimental group and the control group of patients before the start of the chemotherapy is recorded, and the time of the first occurrence of bone marrow suppression, the ratio of bone marrow suppression and the severity of bone marrow suppression in the group of the two groups of patients during the chemotherapy are observed. And meanwhile, an intervention window is set, namely when a certain degree of bone marrow suppression occurs, a certain amount of leucocyte increasing, platelet increasing and hemoglobin increasing are given according to the conventional blood indexes and mental states of the patient according to needs for symptomatic treatment. When symptoms such as fever and infection appear, anti-infection treatment is given according to needs, and the total amount of the treatment medicine used by each group in the chemotherapy cycle is counted.

TABLE 1 bone marrow depression graduation standard table

Remedial drug use conditions: setting an intervention window, namely, when a certain degree of bone marrow suppression appears, according to the conventional blood index and mental state of a patient, a certain amount of leucocyte increasing, platelet increasing and hemoglobin increasing are given according to requirements for symptomatic treatment. When symptoms such as fever and infection appear, anti-infection treatment is given according to needs, and the total amount of the treatment medicine used by each group in the chemotherapy cycle is counted.

And (3) scoring the physical condition: the scores of the patients in the experimental group and the patients in the control group before the chemotherapy of the time are started are recorded by referring to a Kaschin function status scoring table (shown in table 2), and the scores of the two groups of patients in the worst status during the chemotherapy of the time are compared. The higher the score, the better the health condition, and the more tolerable the side effects of the treatment on the body, and thus the likelihood of receiving a complete treatment. The lower the score, the worse the health condition, and below 60, chemotherapy cannot be administered.

TABLE 2 Ka's functional status scoring criteria Table

Chinese medicine symptom scoring: according to the relevant contents in the technical guidance of clinical research on new traditional Chinese medicine of syndrome type (2018), the traditional Chinese medicine syndrome score is actually simulated in clinic (as shown in Table 3). The score was made according to the presence or absence and severity of symptoms, and the score was 1 for asymptomatic, 2 for mild, 3 for moderate and 4 for severe. The scores of the patients in the experimental group and the patients in the control group before the chemotherapy of the time are started are recorded, and the scores of the patients in the two groups at the worst state in the chemotherapy period of the time are compared.

TABLE 3 Chinese medicine syndrome scoring table

The safety index is as follows: the clinical safety of the traditional Chinese medicine composition is judged by comparing the occurrence conditions of other adverse events such as liver and kidney function damage of two groups of patients after chemotherapy through indexes such as glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, urea nitrogen and creatinine.

Routine comparison of peripheral blood before chemotherapy: the leukocyte, neutrophil, platelet and hemoglobin values of the two groups of patients were not statistically different (P > 0.05) and were comparable before chemotherapy, and the results are shown in Table 4.

TABLE 4 comparison of peripheral blood of two groups of patients before chemotherapy (x. + -. S)

Routine comparison of peripheral blood after chemotherapy: after chemotherapy, the values of leucocytes, neutrophils, platelets and hemoglobin of the patients in the control group are all lower than those before chemotherapy, and have statistical difference (P is less than 0.05); the values of the white blood cells and the neutral granulocytes in the experimental group are lower than those before chemotherapy, the statistical difference exists (P is less than 0.05), and the values of the blood platelets and the hemoglobin are not statistically different compared with those before the chemotherapy. The results are shown in Table 5 (comparison in this group before and after chemotherapy, ". DELTA." indicates P < 0.05, ". DELTA." indicates P < 0.01; comparison in both groups after chemotherapy, ". DELTA." indicates P < 0.05).

TABLE 5 comparison of peripheral blood after chemotherapy (x. + -. S) for two groups of patients

Comparison of bone marrow suppression after chemotherapy: after chemotherapy, the control group had 18 cases of myelosuppression, of which 5 cases I, 3 cases II, 1 case III, and 9 cases IV, with an incidence of 60% and an average incidence of myelosuppression of 7.3 days. The experimental group had 11 cases of myelosuppression, including class i 2, class ii 5, class iii 1, and class iv 3, with an incidence of myelosuppression of 37% and an average time of day 10, and the results are shown in table 6.

TABLE 6 comparative analysis of bone marrow suppression in experimental and control groups after chemotherapy

And (3) comparing the remedial administration conditions of the control group and the experimental group: after the completion of chemotherapy and before the next chemotherapy, 13 patients in the control group who used the leucocyte-stimulating drug and 1 patient who used the platelet-stimulating drug were treated. The experimental group had 5 patients who used the leucogen and 1 patient who used the thrombopoietic drug. The number of cases used for remedial treatment in the experimental group was significantly less than that in the control group, and the results are shown in table 7.

TABLE 7 remedial drug use in control and experimental groups

Group of	Total number of cases	Number of cases using leucocyte-stimulating drugs	Number of cases of platelet-producing drugs
				Control group	30	13	1
Experimental group	30	5	1

Comparison of physical strength status of control group and experimental group: before chemotherapy, the Ka's functional status scores of the patients in the control group are evenly divided into 92.2 scores, the Ka's functional status scores of the patients in the experimental group are evenly divided into 91.1 scores, the specific score distribution is shown in Table 8, and the two groups of patients have no significant difference and are comparable. After chemotherapy, the Ka's functional status scores of the patients in the control group were averaged to 75.2 points, the Ka's functional status scores of the patients in the experimental group were averaged to 83.1 points, the scores of the experimental group were significantly higher than those of the control group, and the specific score distribution was shown in Table 9.

TABLE 8 comparison of KPS scores before chemotherapy in two groups of patients

Group of	Total number of cases	90-100	80-89	70-79	60-69	50-59	40-49	Mean value of
									Control group	30	20	10	0	0	0	0	92.2±4.0
Experimental group	30	21	9	0	0	0	0	91.1±6.0

TABLE 9 comparison of KPS scores after chemotherapy in two groups of patients

Group of	Total number of cases	90-100	80-89	70-79	60-69	50-59	40-49	Mean value of
									Control group	30	8	9	2	1	7	3	75.2±17.7ΔΔ
Experimental group	30	19	2	2	4	2	1	83.1±14.7Δ

And (3) comparing the traditional Chinese medicine symptom scores of the control group and the experimental group: before chemotherapy, the scores of the experimental group and the control group in the aspects of 'fatigue and weakness', 'poor appetite and anorexia', 'dizziness and palpitation', 'nausea and vomiting' and 'spontaneous perspiration and night sweat' are not obviously different and are comparable, and the scores are shown in a table 10. After chemotherapy, the scores of all the experimental group and the control group are higher than those before chemotherapy, and the score of all the experimental group is slightly lower than that of the control group, wherein the score of 'spontaneous sweating and night sweat' of the experimental group is obviously lower than that of the control group, and the significant difference (P < 0.05) is shown in table 11.

TABLE 10 comparison of the scores of the syndrome of traditional Chinese medicine before chemotherapy in two groups of patients

Group of

Total number of cases

Mental fatigue and lack of strength

Anorexia and anorexia

Dizziness and palpitation

Nausea and vomiting

Spontaneous perspiration and night sweat

Control group

30

1.13±0.35

1.10±0.31

1.0±0.0

1.03±0.18

1.07±0.25

Experimental group

30

1.10±0.31

1.07±0.25

1.03±0.18

1.0±0.0

1.10±0.31

TABLE 11 comparison of traditional Chinese medicine syndrome scores after chemotherapy for two groups of patients

Comparison of liver and kidney functions of the control group and the experimental group: before chemotherapy, the patients in the experimental group were normal to glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, urea and creatinine, and had no significant difference from the control group, and had comparability, as shown in table 12. After chemotherapy, the values of the indexes of the experimental group and the control group are slightly changed compared with those before chemotherapy, but the patients between the two groups have no significant difference, as shown in table 13.

TABLE 12 comparison of hepatic and renal function of two groups of patients before chemotherapy

Group of	Total number of cases	Glutamic-pyruvic transaminase	Glutamic-oxalacetic transaminase	Urea	Creatinine
						Control group	30	28.9±26.92	26.08±15.28	5.48±1.85	62.19±12.65
Experimental group	30	25.78±16.44	22.76±13.06	5.31±1.22	66.2±13.91

TABLE 13 comparison of liver and kidney function of two groups of patients after chemotherapy

Group of	Total number of cases	Glutamic-pyruvic transaminase	Glutamic-oxalacetic transaminase	Urea	Creatinine
						Control group	30	32.62±22.28	29.59±1.53	5.37±10.3	62.08±17.69
Experimental group	30	42.32±51.98	29.49±22.2	5.62±1.6	66.21±15.06

According to the results of the experimental example 2, the composition for treating deficiency of both qi and blood prepared in example 3 can effectively alleviate the decrease of hemogram caused by chemotherapy of tumor patients, reduce the incidence rate of myelosuppression during chemotherapy, delay the occurrence time of myelosuppression, and has strong effects on alleviating leucopenia and neutropenia caused by chemotherapy. Meanwhile, the composition for treating deficiency of qi and blood prepared in the embodiment 3 can relieve the aggravation of the traditional Chinese medicine symptoms caused by chemotherapy, and particularly has a good effect on spontaneous perspiration and night sweat. In addition, the traditional Chinese medicine composition has no obvious toxicity of liver and kidney function damage.

In conclusion, the composition for treating deficiency of qi and blood provided by the embodiment of the invention adopts a plurality of medicinal materials for replenishing blood and tonifying qi, and the medicinal materials with cold and warm natures are mutually harmonized and restricted according to a certain proportion, so that the effect of harmonizing yin and yang is achieved; in addition, the medicinal materials are combined according to a reasonable monarch, minister, assistant and guide relationship, so the composition for treating deficiency of qi and blood provided by the invention has a better curative effect on treating symptoms causing deficiency of qi and blood, such as aplastic anemia and other anemia symptoms, and the composition for treating deficiency of qi and blood can also clear heat and dry dampness, nourish yin and tonify yang, invigorate stomach and promote digestion, arrest seminal emission and arrest spermatorrhea, and regulate the physical condition from multiple aspects.

The adjuvant drugs comprise fructus Lycii, fructus Ligustri Lucidi processed with wine, Saviae Miltiorrhizae radix, caulis Spatholobi, endothelium corneum Gigeriae Galli preparata, fructus Hordei Germinatus preparata, herba Epimedii, herba et Gemma Agrimoniae, Coptidis rhizoma, radix rehmanniae and Notoginseng radix powder, and fructus Lycii is mature fruit of fructus Lycii, has sweet taste and mild property, can nourish liver and kidney, and benefit essence and improve eyesight, and is used for treating deficiency of essence and blood and deficiency of liver and kidney; the wine glossy privet fruit is mainly prepared from ripe glossy privet fruits, is sweet and bitter in taste and mild in nature, can nourish yin, prolong life, tonify liver and kidney, and is mainly used for treating symptoms such as light headedness and the like caused by qi and blood deficiency; the root and the stem of the salvia miltiorrhiza are mainly selected as the raw materials, the salvia miltiorrhiza is bitter in taste and slightly cold in nature, can tonify qi and activate blood, and is mainly used for treating symptoms such as chest stuffiness, pains, vexation, insomnia and the like; the caulis spatholobi is mainly used as a medicine for rattan stems, is bitter and sweet in taste and warm in nature, can promote blood circulation and enrich the blood, and is mainly used for treating blood deficiency and chlorosis and other symptoms; the fried endothelium corneum Gigeriae Galli is prepared by frying fresh endothelium corneum Gigeriae Galli, has sweet taste and mild property, can invigorate stomach, promote digestion, astringe essence and relieve enuresis, and is mainly used for treating indigestion, enuresis and spermatorrhea; the fried malt is prepared by frying malt, can promote qi circulation, promote digestion, invigorate spleen and stimulate appetite, and is mainly used for treating symptoms such as indigestion, spleen deficiency and anorexia; the epimedium is mainly selected from the leaves of the epimedium as a medicine, has pungent and sweet taste and warm nature, can tonify kidney yang, and is mainly used for treating symptoms such as kidney yang deficiency and the like; the hairyvein agrimony is mainly selected from stems and leaves thereof to be used as a medicine, has bitter and astringent taste and mild property, can stop bleeding and tonify deficiency, and is mainly used for treating symptoms such as metrorrhagia, metrostaxis and bleeding; the coptis is mainly selected from dried rhizome parts of the coptis as a medicine, is bitter in taste and cold in nature, can clear heat and dry dampness, and is mainly used for treating symptoms such as blood heat hematemesis and epistaxis; dried root tubers of the dried rehmannia root are mainly selected as the raw materials, are sweet in taste and slightly warm in nature, can enrich blood, nourish yin, replenish vital essence and fill marrow, and are mainly used for treating blood deficiency, chlorosis, liver and kidney yin deficiency and other symptoms; the notoginseng is mainly selected from dried root thereof as a medicine, has sweet and slightly bitter taste and warm nature, can remove blood stasis and stop bleeding, and activate blood and relieve pain, and is mainly used for treating symptoms such as traumatic hemorrhage, hematochezia and the like. The medlar and the wine glossy privet fruit can cooperate with the tortoise-shell glue, the antler glue and the angelica to tonify the essence and blood of the liver and kidney, the epimedium and the antler glue can warm the kidney and strengthen yang, and have the effect of reinforcing the yang and invigorating the yin by being matched with various medicinal materials for nourishing yin and blood in the adjuvant drugs, and the coptis chinensis is cold in nature, can harmonize the property of the warm drugs and has the efficacy of cooperating with hemostasis. In addition, the composition for treating deficiency of qi and blood can remarkably improve erythroid cells, strengthen the hematopoietic function of a human body and the blood transportation function; and has remarkable curative effect on splenomegaly.

The invention also provides a preparation method of the composition for treating deficiency of both qi and blood, which adopts the steps of decoction, concentration and paste collection, and can reduce the volume of the composition for treating deficiency of both qi and blood, so that the composition is convenient to take and the drug property of the composition is improved; and the preparation method is simple and convenient to operate, is easy to operate, and is convenient for operators to operate.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. The composition for treating deficiency of qi and blood is characterized by being mainly prepared from the following raw materials in parts by weight: 75-85 parts of astragalus membranaceus, 25-35 parts of salvia miltiorrhiza, 55-65 parts of codonopsis pilosula, 40-50 parts of wolfberry fruit, 85-95 parts of hairyvein agrimony, 85-95 parts of caulis spatholobi, 45-55 parts of radix rehmanniae recen, 25-35 parts of roasted malt, 35-45 parts of roasted bighead atractylodes rhizome, 55-65 parts of angelica sinensis, 15-25 parts of roasted chicken's gizzard-membrane, 40-50 parts of wine glossy privet fruit, 15-25 parts of epimedium herb, 10-20 parts of coptis chinensis, 25-35 parts of deerhorn glue, 25-35 parts of tortoise-shell glue and 5-15 parts of pseudo.

2. The composition for treating deficiency of both qi and blood according to claim 1, which is prepared from the following raw materials in parts by weight: 80 parts of astragalus, 30 parts of salvia miltiorrhiza, 60 parts of codonopsis pilosula, 45 parts of wolfberry, 90 parts of hairyvein agrimony, 90 parts of suberect spatholobus stem, 50 parts of radix rehmanniae recen, 30 parts of fried malt, 40 parts of fried bighead atractylodes rhizome, 60 parts of Chinese angelica, 20 parts of fried chicken gizzard membrane, 45 parts of wine glossy privet fruit, 20 parts of epimedium herb, 15 parts of coptis chinensis, 30 parts of deerhorn glue, 30 parts of tortoise-shell glue and 10 parts of pseudo-ginseng powder.

3. Use of a composition according to claim 1 or 2 for the treatment of deficiency of both qi and blood in the manufacture of a medicament for the treatment of anemia and platelet, leukopenia and hemogram recovery.

4. A method for preparing a composition for treating deficiency of both qi and blood according to claim 1 or 2, comprising the steps of: soaking the other raw materials except the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi in water, decocting to obtain a decoction, concentrating the decoction to obtain a concentrated solution, and adding the pseudo-ginseng powder, the colla cornus cervi and the colla cornus cervi into the concentrated solution to obtain paste, thus obtaining the composition for treating deficiency of qi and blood.

5. The method for preparing the composition for treating deficiency of both qi and blood as claimed in claim 4, further comprising a sterilization step before said soaking, wherein said sterilization step comprises exposing said raw materials to 255-265nm ultraviolet light for 10-20 min.

6. The method for preparing a composition for treating deficiency of both qi and blood according to claim 4, wherein the tortoise shell glue and antler glue are further processed by sand-scalding to make them crisp before being added to the concentrated solution.

7. The preparation method of the composition for treating deficiency of both qi and blood according to claim 4, wherein the decoction is divided into two times, the first decoction is carried out for 40-50min, and the first decoction is obtained after filtration; adding water again for second decoction, wherein the time for the second decoction is 40-50min, filtering to obtain a second decoction, and mixing the first decoction and the second decoction.

8. The method for preparing a composition for treating deficiency of both qi and blood according to claim 6, wherein the amount of water added in the first decoction and the amount of water added in the second decoction are respectively 12 times of the weight of the added medicinal materials, and the first decoction and the second decoction are both boiled with strong fire and then slowly decocted with slow fire.

9. The method for preparing a composition for treating deficiency of both qi and blood according to claim 4, wherein the relative density of the concentrate at the time of concentration is controlled to be 1.02 to 1.1 at 60 ℃.

10. The method for preparing a composition for treating deficiency of both qi and blood according to claim 4, wherein the relative density of the composition for treating deficiency of both qi and blood at the time of harvesting the paste is 1.1 to 1.2 at 60 ℃.