CN112626043B - Rumen bypass glucose oxidase and preparation method and use method thereof - Google Patents
Rumen bypass glucose oxidase and preparation method and use method thereof Download PDFInfo
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- CN112626043B CN112626043B CN202011612475.8A CN202011612475A CN112626043B CN 112626043 B CN112626043 B CN 112626043B CN 202011612475 A CN202011612475 A CN 202011612475A CN 112626043 B CN112626043 B CN 112626043B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03004—Glucose oxidase (1.1.3.4)
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Abstract
The invention provides rumen bypass glucose oxidase, which comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase. The beneficial effects of the invention are as follows: the feed additive has the advantages of green and safe components, no drug residue, and effective improvement of intestinal health of ruminant, and milk yield and milk production performance of dairy cows.
Description
Technical Field
The invention relates to the field of preparation of rumen bypass glucose oxidase, in particular to rumen bypass glucose oxidase, and a preparation method and a use method thereof.
Background
The glucose oxidase is an aerobic dehydrogenase, and specifically oxidizes beta-D-glucose to gluconic acid and H 2 0 2 While consuming oxygen. In the presence of catalase, hydrogen peroxide is decomposed to form water.
Glucose oxidase plays an important role in animal production. It can improve intestinal health of livestock and fowl and improve immunity; can inhibit the growth of intestinal pathogenic bacteria, inhibit coccidium invasion, reduce intestinal diseases, improve the condition of livestock manure, obviously reduce the ammonia concentration of the livestock house and improve the environment of the livestock house; can relieve the harm of mould and mycotoxin, maintain the health of livestock and poultry, and improve the production performance and comprehensive cultivation benefit of livestock and poultry.
In ruminant application, on one hand, the glucose oxidase can improve the production performance of cows, effectively relieve common inappetence phenomenon caused by perinatal period, calf grass replacement and diseases of cows, and also has the effects of preventing retained fetal membranes, reducing metabolic diseases, improving long-term dyspepsia of cows and the like. On the other hand, the glucose oxidase improves the quality of silage, because the glucose oxidase can consume oxygen in the silage process, the anaerobic lactobacillus proliferation is facilitated, the lactobacillus fermentation process is accelerated, the silage pH value is rapidly reduced, the harmful bacteria proliferation is inhibited, the abnormal fermentation is avoided, and finally the silage quality is ensured. The chemical nature of glucose oxidase is a protein that enters the rumen and is easily degraded by rumen microorganisms, most of which lose their intended functions.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides rumen bypass glucose oxidase, and a preparation method and a use method thereof.
The aim of the invention is achieved by the following technical scheme.
Rumen bypass glucose oxidase comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase.
Preferably, the weight percentages of the components are: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
A method for using rumen bypass glucose oxidase comprises mixing rumen bypass glucose oxidase 100-200mg per kg body weight per day with fine feed supplement, and feeding.
A preparation method of rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting palm fat powder, and then keeping the temperature to 55 ℃ for later use;
step two, glucose oxidase, lipase and silicon dioxide are taken and evenly mixed with the palm fat powder prepared in the step one, and then the mixture is sieved by a sieve of 80 meshes, and the mixture is transferred into a centrifugal granulator for stirring, and is taken out after a certain time and is sieved by a sieve of 60 meshes;
thirdly, taking core particles with the particle size less than or equal to 60 meshes in the second step, placing the core particles in a centrifugal granulator again, and slowly adding calcium stearate and the palm fat powder prepared in the first step to prepare the rumen bypass glucose oxidase.
Preferably, the glucose oxidase added in the second step is 35% -45% by weight.
Preferably, in any of the above embodiments, the glucose oxidase is added in the second step in an amount of 40% by weight.
Preferably, in any of the above embodiments, the lipase is added in the second step in an amount of 1% -3% by weight.
Preferably, in any of the above embodiments, the lipase is added in the second step in an amount of 2% by weight.
Preferably, in any of the above embodiments, the silica added in the second step is 7% to 9% by weight.
Preferably, in any of the above embodiments, the silica is present in an amount of 8% by weight.
Preferably, in any of the above embodiments, the weight percentage of the palm fat powder added in the second step is 34%.
Preferably, in any of the above embodiments, the weight percentage of the calcium stearate added in the third step is 5% -7%.
Preferably, in any of the above embodiments, the weight percentage of the calcium stearate is 6%.
Preferably, in any of the above embodiments, the weight percentage of the palm fat powder added in the third step is 10%.
The beneficial effects of the invention are as follows:
the rumen bypass glucose oxidase product prepared by the method is yellow or pale yellow granular substances, mainly comprises glucose oxidase and protective materials, and has the active ingredient content of more than 40 percent. The feed additive is prepared by adopting the protection of plant-derived fatty acid through a coating technology, so that the product has high safety and no side effect, can be directly added into feed for feeding, and can be used as a feed additive to be added into various ruminant feeds.
Detailed Description
The technical scheme of the invention is further described by specific examples.
1. Rumen bypass glucose oxidase formula
Rumen bypass glucose oxidase comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase.
Preferably, the weight percentages of the components are: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
The glucose oxidase and the lipase meet the feed-grade additive standard, the palm fat powder meets the feed-grade standard, and the silicon dioxide and the calcium stearate meet the food-grade additive standard.
2. Preparation method of rumen bypass glucose oxidase
A preparation method of rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting palm fat powder, and then keeping the temperature to 55 ℃ for later use;
step two, taking 40% of glucose oxidase, 2% of lipase and 8% of silicon dioxide, uniformly mixing with 34% of palm fat powder prepared in the step one, sieving with a 80-mesh sieve, transferring into a centrifugal granulator, stirring for a certain time, taking out, and sieving with a 60-mesh sieve;
thirdly, core particles with the particle size less than or equal to 60 meshes in the second step are taken and placed in a centrifugal granulator again, 6% of calcium stearate and 10% of palm fat powder prepared in the first step are slowly added, and rumen bypass glucose oxidase is finally prepared.
As is clear from Table 1, the above method uses glucose oxidase with a primary enzyme activity of 12000U/g, and the prepared rumen bypass glucose oxidase has a glucose oxidase ratio of 40%, the enzyme activity is 4800U/g, the actual activity is 4251.5U/g, the enzyme activity retention rate is 88.6%, only 11.4% is lost in the coating process, and the enzyme activity retention rate is high.
TABLE 1 rumen bypass glucose oxidase retention assay
3. Test of Using Effect of rumen-bypass glucose oxidase additive
1 test method
1.1 design of experiments
The test is carried out by three treatment groups, namely a control group, a test 1 group and a test 2 group, wherein the control group and the control group are fed with basic daily ration (the ratio of the refined daily ration to the coarse daily ration is 50:50), and the test 1 group and the test 2 group are uniformly mixed with the basic daily ration according to the amounts of 100 mg/(kg x d) and 200 mg/(kg x d), respectively. The rumen bypass glucose oxidase additive consists of the following components in proportion: 40% of raw material enzyme, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
The feeding test was performed for 10 days in the pre-feeding period and 60 days in the positive test period.
1.2 test animals
60 Holstein cows with similar weight, number of fetuses and lactation amount are selected and randomly divided into 3 groups of 20 replicates each, and 1 replicate each.
1.3 test diet and feeding management
The test diet is prepared according to the nutrition requirement and the feeding standard of dairy cows, and consists of mixed concentrate and corn silage, and the composition and the nutrition ingredients are shown in Table 2. The ratio of the fine to the coarse of the diet is 50:50, the test cows are fed in a single groove, fed in a daily way of 07:00 and 19:00, and eat and drink water freely.
Table 2 test of basal diet composition and nutrient level
Each kilogram of premix comprises: 100mg cobalt, 8500mg copper, 50,000mg iron, 30,000mg manganese, 300mg zinc, 300mg iodine, 300mg selenium, 7500,000IU vitamin A, 1200,000IU vitamin D and 40,000IU vitamin E.
1.4 sample collection and measurement
1.4.1 feed intake determination and feed sample acquisition and analysis
The feed intake and the residual forage amount are recorded in detail daily during the test period, concentrate and corn silage samples are collected in proportion daily, and the concentrate and corn silage samples are stored for standby after primary water is measured.
1.4.2 determination of milk production Performance index
During the test, the milk yield of each cow is recorded every day, 50mL of milk samples are collected according to the ratio of 4:3:3 of the morning, the evening and the evening, 0.6mg/mL of potassium dichromate is added, and the mixture is cooled at 4 ℃ after being uniformly mixed for standby measurement. And (3) sending the collected milk sample to a detection center, and detecting the milk protein rate, lactose rate, milk dry matter rate and somatic cell number by using a multifunctional milk component analyzer.
2 test results
2.1 Effect of rumen bypass glucose oxidase on Holstein cow production Performance
As can be seen from table 3, the average daily milk yield in the test 1 group and the test 2 group was significantly higher than that in the control group, and the average daily feed intake had no significant effect but had a tendency to increase. The average daily milk yield of the test 1 group and the test 2 group was increased by 12.1% and 20.7%, respectively.
TABLE 3 influence of rumen bypass glucose oxidase on Holstein cow production performance
Note that: the same letters abc in the same row show that the difference is not significant (P > 0.05), and the letters differ significantly (P < 0.05).
2.2 Effect of rumen bypass glucose oxidase on milk production performance of Holstein cows
As can be seen from table 4, there was no significant difference between the milk protein rate and the non-fat solids of each experimental group. The lactose rate and total milk solids were significantly higher in the test 1 and test 2 groups than in the control group, the milk fat rate was significantly higher than in the control group, and the somatic cell count was significantly lower than in the control group.
TABLE 4 influence of rumen bypass glucose oxidase on milk production performance of Holstein cows
Note that: the same letters abc in the same row show that the difference is not significant (P > 0.05), and the letters differ significantly (P < 0.05).
3 conclusion of the test
The rumen bypass glucose oxidase additive can improve the milk yield of Holstein cows, improve the milk quality and improve the milk production performance.
Example 1
The rumen bypass glucose oxidase comprises 400g of glucose oxidase which accords with the standard of a feed-grade additive, 20g of lipase, 440g of palm fat powder which accords with the standard of the feed-grade additive, 80g of silicon dioxide which accords with the standard of the food-grade additive and 60g of calcium stearate.
The preparation method of rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting 440g of palm fat powder, and then keeping the temperature to 55 ℃ for later use;
step two, taking 400g of glucose oxidase, 20g of lipase and 80g of silicon dioxide, uniformly mixing with 340g of the heated and melted palm fat powder obtained in the step one, sieving with a 80-mesh sieve, transferring into a centrifugal granulator, stirring for a certain time, taking out, and sieving with a 60-mesh sieve;
thirdly, placing the core particles with the particle size not larger than 60 meshes in the second step in a centrifugal granulator again, slowly adding 60g of calcium stearate and 100g of the heated and melted palm fat powder obtained in the first step, and finally preparing the rumen bypass glucose oxidase.
Example 2
The rumen bypass glucose oxidase comprises 420g of glucose oxidase, 15g of lipase, 450g of palm fat powder, 90g of silicon dioxide and 70g of calcium stearate.
The raw material standard and the preparation method in this example are the same as in example 1.
Example 3
The rumen bypass glucose oxidase comprises 450g of glucose oxidase, 20g of lipase, 480g of palm fat powder, 90g of silicon dioxide and 70g of calcium stearate.
The raw material standard and the preparation method in this example are the same as in example 1.
Example 4
The rumen bypass glucose oxidase comprises 350g of glucose oxidase, 10g of lipase, 400g of palm fat powder, 70g of silicon dioxide and 60g of calcium stearate.
The raw material standard and the preparation method in this example are the same as in example 1.
The four embodiments of the present invention have been described in detail, but the description is only a preferred embodiment of the present invention and should not be construed as limiting the scope of the invention. All equivalent changes and modifications within the scope of the present invention are intended to be covered by the present invention.
Claims (2)
1. A rumen bypass glucose oxidase, characterized in that: the weight percentages of the components are as follows: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
2. A preparation method of rumen bypass glucose oxidase is characterized in that: the method comprises the following steps: firstly, heating and melting palm fat powder, and then keeping the temperature to 55 ℃ for later use; step two, glucose oxidase, lipase and silicon dioxide are taken and evenly mixed with the palm fat powder prepared in the step one, and then the mixture is sieved by a sieve of 80 meshes, and the mixture is transferred into a centrifugal granulator for stirring, taken out and sieved by a sieve of 60 meshes; thirdly, taking core particles with the particle size less than or equal to 60 meshes in the second step, placing the core particles in a centrifugal granulator again, and slowly adding calcium stearate and the palm fat powder prepared in the first step to prepare rumen bypass glucose oxidase; the weight percentage of the glucose oxidase added in the second step is 40%; the weight percentage of the lipase added in the second step is 2%; the weight percentage of the silicon dioxide is 8%; the weight percentage of the palm fat powder added in the second step is 34%; the weight percentage of the calcium stearate is 6%; the weight percentage of the palm fat powder added in the third step is 10 percent.
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Citations (4)
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| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN109619288A (en) * | 2018-12-11 | 2019-04-16 | 北京东方天合生物技术有限责任公司 | A kind of novel rumen glucose and the preparation method and application thereof |
| CN110637930A (en) * | 2019-06-11 | 2020-01-03 | 武瑞 | Rumen-protected amylase and preparation method thereof |
| CN111543550A (en) * | 2020-05-12 | 2020-08-18 | 肖鹏宏 | Whole-daily-grain compound feed for dairy cow feeding without grass and preparation method thereof |
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| US7618670B2 (en) * | 2004-06-14 | 2009-11-17 | Premium Vegetable Oils Sdn. Bhd. | Trans free non-hydrogenated hard structural fat and non-hydrogenated hard palm oil fraction component |
| WO2018218020A1 (en) * | 2017-05-24 | 2018-11-29 | Rupca Llc | Reduced pressure maillard synthesis of carbohydrate energy supplement for ruminant livestock |
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|---|---|---|---|---|
| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN109619288A (en) * | 2018-12-11 | 2019-04-16 | 北京东方天合生物技术有限责任公司 | A kind of novel rumen glucose and the preparation method and application thereof |
| CN110637930A (en) * | 2019-06-11 | 2020-01-03 | 武瑞 | Rumen-protected amylase and preparation method thereof |
| CN111543550A (en) * | 2020-05-12 | 2020-08-18 | 肖鹏宏 | Whole-daily-grain compound feed for dairy cow feeding without grass and preparation method thereof |
Non-Patent Citations (1)
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| 外源酶提高反刍动物饲料利用率的研究进展;刘芳;《反刍动物营养》;20061231;第27卷(第23期);全文 * |
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