CN112625926A - Microbial preparation for treating household kitchen garbage and application thereof - Google Patents
Microbial preparation for treating household kitchen garbage and application thereof Download PDFInfo
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- CN112625926A CN112625926A CN202011602685.9A CN202011602685A CN112625926A CN 112625926 A CN112625926 A CN 112625926A CN 202011602685 A CN202011602685 A CN 202011602685A CN 112625926 A CN112625926 A CN 112625926A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention discloses a microbial preparation for treating household kitchen garbage and application thereof. The microbial agent is prepared from raw materials consisting of compound microorganisms, molasses, glutamic acid, urea and clear water. The raw materials comprise 1 to 5 portions of compound microorganism, 0.5 to 5 portions of molasses, 0.2 to 5 portions of glutamic acid, 1 to 5 portions of urea and 80 to 95 portions of clear water according to weight. When the microbial preparation is applied, the microbial preparation is uniformly sprayed on kitchen garbage, then the kitchen garbage is sealed and fermented for 30-40 days at the temperature of 20 ℃ to obtain the biological organic fertilizer. The microbial preparation can degrade organic matters in the kitchen waste, convert the kitchen waste into the biological organic fertilizer, realize resource utilization of the kitchen waste and reduce environmental pollution.
Description
Technical Field
The invention belongs to the technical field of household garbage treatment, and particularly relates to a microbial preparation for treating household kitchen garbage and application thereof.
Background
Along with the development of urbanization in China and the improvement of living standard of people, the amount of kitchen garbage in a living community is increasing day by day. If the wastes are not treated in time, the wastes occupy the space of a community, even decay, deteriorate and breed pathogenic bacteria, and the living environment and the body health of residents are seriously influenced. However, kitchen waste contains a large amount of organic substances, which are also a usable resource.
During the metabolism activity of the microorganism, a large amount of physiologically active substances (such as protease, amylase, cellulase and the like) can be generated, and the kitchen waste can be degraded by the substances and then converted into fertilizer, so that the recycling of the kitchen waste can be realized, and the problem of related environmental pollution can be solved.
Therefore, the development of the microbial preparation containing beneficial microorganisms and the application of the microbial preparation to the treatment of the kitchen waste of residents are significant to the sustainable development of cities.
Disclosure of Invention
The invention aims to provide a microbial preparation for treating kitchen waste of residents and application thereof, which can reduce environmental pollution of the kitchen waste of residents and realize resource utilization of the kitchen waste.
On one hand, the invention provides a microbial preparation for treating household kitchen waste, which is prepared from raw materials consisting of compound microorganisms, molasses, glutamic acid, urea and clear water.
Preferably, the components of the raw materials are uniformly mixed and then fermented at the temperature of 18-28 ℃, and when the pH of the fermented material is less than or equal to 4.5, the fermentation is finished to obtain the microbial preparation.
Preferably, the raw material consists of 1-5 parts of the compound microorganism, 0.5-5 parts of the molasses, 0.2-5 parts of the glutamic acid, 1-5 parts of the urea and 80-95 parts of the clean water by weight.
Preferably, the raw material consists of 1 part of the compound microorganism, 0.2 part of the molasses, 3.5 parts of the glutamic acid, 3 parts of the urea and 80 parts of the clean water by weight.
Preferably, the raw material consists of 2 parts of the compound microorganism, 2.3 parts of the molasses, 2.5 parts of the glutamic acid, 4 parts of the urea and 86 parts of the clean water by weight.
Preferably, the raw material consists of 4 parts of the compound microorganism, 3.7 parts of the molasses, 5 parts of the glutamic acid, 1 part of the urea and 91 parts of the clean water by weight.
Preferably, the raw material consists of 5 parts of the compound microorganism, 5 parts of the molasses, 0.2 part of the glutamic acid, 5 parts of the urea and 95 parts of the clean water by weight.
Preferably, the complex microorganisms include verticillium, verticillium chlamydosporium, lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis.
The invention also provides application of the microbial preparation, wherein the microbial preparation is uniformly sprayed on kitchen garbage of residents, then the kitchen garbage is sealed and fermented for 30-40 days at the temperature of 20 ℃ to obtain the biological organic fertilizer.
Preferably, the mass ratio of the microbial preparation to the kitchen waste is 1:500-1: 50.
The microbial preparation for treating the resident kitchen waste can degrade organic matters in the kitchen waste, convert the kitchen waste into the bio-organic fertilizer, realize resource utilization of the kitchen waste and reduce environmental pollution.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention mixes the compound microorganism, molasses, glutamic acid, urea and clean water according to a certain proportion, and then mixes and ferments to obtain the microbial preparation for treating the kitchen waste of residents. The microbial preparation is applied to resident kitchen waste, can degrade organic matters in the kitchen waste, converts the kitchen waste into a biological organic fertilizer, realizes resource utilization of the kitchen waste, and reduces environmental pollution.
In one embodiment of the invention, the microbial preparation for treating the household kitchen waste is prepared by adopting raw materials consisting of compound microorganisms, molasses, glutamic acid, urea and clear water, and the specific preparation process comprises the following steps:
step 1, selecting a bacterial liquid of a yellow-blue bacterium (Talaromyces flavus), a bacterial liquid of a chlamydosporium (Verticilliumchlamydospora), a bacterial liquid of Lactobacillus acidophilus (Lactobacillus acidophilus), a bacterial liquid of Saccharomyces cerevisiae (Saccharomyces cerevisiae) and a bacterial liquid of Bacillus subtilis, and then compounding the five bacterial liquids according to a proportioning relationship by weight to obtain a liquid composite microorganism, wherein the liquid composite microorganism is preferably 5-15 parts of the yellow-blue bacterium liquid, 1-10 parts of the chlamydosporium bacterial liquid, 15-25 parts of the Lactobacillus acidophilus bacterial liquid, 10-20 parts of the Saccharomyces cerevisiae bacterial liquid and 5-15 parts of the Bacillus subtilis liquid. On one hand, lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media respectively to obtain bacterial liquids, and then the bacterial liquids are concentrated to prepare single-strain high-concentration bacterial liquids. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the bacteria content of Lactobacillus acidophilus is not less than 4.0 × 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 3.0 multiplied by 1010cfu/mL. On the other hand, the verticillium dahliae and verticillium chlamydosporium are fermented in corresponding liquid culture media to obtain bacterial liquids, and then the bacterial liquids are concentrated to prepare single-bacterial-strain high-concentration bacterial liquids. The bacterial contents of the two single bacterial strains are respectively as follows: the content of yellow blue fungus is not less than 2.0 × 1010cfu/mL, the bacterial content of Verticillium chlamydosporium is more than or equal to 2.5 multiplied by 1010cfu/mL。
And 2, weighing various raw material components according to a preset weight ratio, adding the raw material components together, and stirring and mixing uniformly to obtain a mixed material. The weight ratio of each component in the raw materials is respectively optimized as follows: 1-5 parts of compound microorganism, 0.5-5 parts of molasses, 0.2-5 parts of glutamic acid, 1-5 parts of urea and 80-95 parts of clear water.
And 3, performing fermentation at the temperature of 18-28 ℃ after the initial pH of the mixed material is natural, and finishing the fermentation when the pH of the fermented material is less than or equal to 4.5 to obtain the microbial preparation for treating the kitchen waste of the residents.
In another embodiment of the invention, when the microbial preparation is used for treating the kitchen waste of residents, the microbial preparation and the kitchen waste are uniformly sprayed on the kitchen waste of residents according to the application amount of the microbial preparation to the kitchen waste of 1:500-1:50 by mass, then the kitchen waste is sealed, preferably the kitchen waste is put into a plastic bag and fermented for 30-40 days at the temperature of 20 ℃ to obtain the bio-organic fertilizer.
To help better understand the technical solution of the present invention, the following examples are provided to illustrate the preparation process of the microbial preparation of the present invention and the method of using the same.
Example one
The raw materials of the microbial preparation of the embodiment comprise 1 part of compound microorganism, 0.2 part of molasses, 3.5 parts of glutamic acid, 3 parts of urea and 80 parts of clear water by weight. The compound microorganism is formed by mixing a yellow-blue bacterium liquid, a verticillium chlamydosporium bacterium liquid, a lactobacillus acidophilus bacterium liquid, a saccharomyces cerevisiae bacterium liquid and a bacillus subtilis bacterium liquid, wherein the five bacterium liquids are 5 parts of the yellow-blue bacterium liquid, 4 parts of the verticillium chlamydosporium bacterium liquid, 25 parts of the lactobacillus acidophilus bacterium liquid, 10 parts of the saccharomyces cerevisiae bacterium liquid and 11 parts of the bacillus subtilis bacterium liquid respectively. The bacteria content of the five bacteria liquids is respectively as follows: the content of yellow blue fungus is not less than 2.0 × 1010cfu/mL, the bacterial content of Verticillium chlamydosporium is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of lactobacillus acidophilus is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 3.0 multiplied by 1010cfu/mL。
The microbial preparation is prepared by the following steps.
Step 1, uniformly mixing a yellow-blued bacterium liquid, a verticillium chlamydosporium bacterium liquid, a lactobacillus acidophilus bacterium liquid, a saccharomyces cerevisiae bacterium liquid and a bacillus subtilis bacterium liquid to obtain the composite microorganism.
And 2, adding the compound microorganism, molasses, glutamic acid, urea and clear water together, and stirring and mixing uniformly to obtain a mixed material.
And 3, performing fermentation at the temperature of 18-28 ℃ after the initial pH of the mixed material is natural, and finishing the fermentation when the pH of the fermented material is less than or equal to 4.5 to obtain the microbial preparation 1 for treating the kitchen waste of the residents.
The application method of the microbial preparation 1 comprises the following steps: when the resident kitchen waste is treated, the microbial preparation and the kitchen waste are uniformly sprayed on the kitchen waste according to the application amount of 1:500 of the mass ratio of the microbial preparation to the kitchen waste, then the kitchen waste is put into a plastic bag and fermented for 40 days at the temperature of 20 ℃ to obtain the bio-organic fertilizer.
Example two
The raw materials of the microbial preparation of the embodiment comprise 2 parts of compound microorganism, 2.3 parts of molasses, 2.5 parts of glutamic acid, 4 parts of urea and 86 parts of clear water by weight. The compound microorganism is formed by mixing 9 parts of a yellow-blue bacterium solution, 1 part of a chlamydosporium bacterium solution, 21 parts of an acidophilic lactobacillus bacterium solution, 14 parts of a saccharomyces cerevisiae bacterium solution and 7 parts of a bacillus subtilis solution. The bacteria content of the five bacteria liquids is respectively as follows: the content of yellow blue fungus is not less than 2.0 × 1010cfu/mL, the bacterial content of Verticillium chlamydosporium is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of lactobacillus acidophilus is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 3.0 multiplied by 1010cfu/mL。
The microbial preparation 2 of this example was prepared in the same manner as in example 1.
The application method of the microbial preparation 2 comprises the following steps: when the resident kitchen waste is treated, the microbial preparation and the kitchen waste are uniformly sprayed on the kitchen waste according to the application amount of 1:200 mass ratio, then the kitchen waste is put into a plastic bag and fermented for 37 days at the temperature of 20 ℃ to obtain the bio-organic fertilizer.
EXAMPLE III
The raw material of the microbial preparation of this example was a complex microorganism by weight4 parts of molasses 3.7 parts, glutamic acid 5 parts, urea 1 part and clean water 91 parts. The compound microorganism is formed by mixing a yellow-blue bacterium liquid, a verticillium chlamydosporia bacterium liquid, a lactobacillus acidophilus bacterium liquid, a saccharomyces cerevisiae bacterium liquid and a bacillus subtilis bacterium liquid, wherein the five bacterium liquids are 12 parts of the yellow-blue bacterium liquid, 7 parts of the verticillium chlamydosporia bacterium liquid, 17 parts of the lactobacillus acidophilus bacterium liquid, 16 parts of the saccharomyces cerevisiae bacterium liquid and 5 parts of the bacillus subtilis bacterium liquid respectively. The bacteria content of the five bacteria liquids is respectively as follows: the content of yellow blue fungus is not less than 2.0 × 1010cfu/mL, the bacterial content of Verticillium chlamydosporium is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of lactobacillus acidophilus is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 3.0 multiplied by 1010cfu/mL。
The microbial preparation 3 of this example was prepared in the same manner as in example 1.
The application method of the microbial preparation 3 comprises the following steps: when the resident kitchen waste is treated, the microbial preparation and the kitchen waste are uniformly sprayed on the kitchen waste according to the application amount of 1:100 of the mass ratio of the microbial preparation to the kitchen waste, then the kitchen waste is put into a plastic bag and fermented for 33 days at the temperature of 20 ℃ to obtain the bio-organic fertilizer.
Example four
The raw materials of the microbial preparation of the embodiment are 5 parts of compound microorganism, 5 parts of molasses, 0.2 part of glutamic acid, 5 parts of urea and 95 parts of clear water by weight. The compound microorganism is formed by mixing a yellow-blue bacterium liquid, a verticillium chlamydosporium bacterium liquid, a lactobacillus acidophilus bacterium liquid, a saccharomyces cerevisiae bacterium liquid and a bacillus subtilis bacterium liquid, wherein the five bacterium liquids are 15 parts of the yellow-blue bacterium liquid, 10 parts of the verticillium chlamydosporium bacterium liquid, 15 parts of the lactobacillus acidophilus bacterium liquid, 20 parts of the saccharomyces cerevisiae bacterium liquid and 15 parts of the bacillus subtilis bacterium liquid respectively. The bacteria content of the five bacteria liquids is respectively as follows: the content of yellow blue fungus is not less than 2.0 × 1010cfu/mL, the bacterial content of Verticillium chlamydosporium is more than or equal to 2.5 multiplied by 1010cfu/mL, the bacterium content of lactobacillus acidophilus is more than or equal to 4.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 2.5 multiplied by 1010cfu/mL, containing bacteria of Bacillus subtilisThe amount is more than or equal to 3.0 multiplied by 1010cfu/mL。
The microbial preparation 4 of this example was prepared in the same manner as in example 1.
The application method of the microbial preparation 4 comprises the following steps: when the resident kitchen waste is treated, the microbial preparation and the kitchen waste are uniformly sprayed on the kitchen waste according to the application amount of 1:50 of the mass ratio of the microbial preparation to the kitchen waste, then the kitchen waste is put into a plastic bag and fermented for 30 days at the temperature of 20 ℃ to obtain the bio-organic fertilizer.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications made by the present invention in the equivalent structure or directly or indirectly applied to other related technical fields are within the scope of the present invention.
Claims (10)
1. A microbial preparation for treating kitchen garbage of residents is prepared from raw materials consisting of compound microorganisms, molasses, glutamic acid, urea and clear water.
2. The microbial formulation of claim 1, wherein: uniformly mixing the components of the raw materials, fermenting at the temperature of 18-28 ℃, and ending fermentation when the pH of the fermented material is less than or equal to 4.5 to obtain the microbial preparation.
3. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 1-5 parts of the compound microorganism, 0.5-5 parts of the molasses, 0.2-5 parts of the glutamic acid, 1-5 parts of the urea and 80-95 parts of the clean water.
4. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise 1 part of the compound microorganism, 0.2 part of molasses, 3.5 parts of glutamic acid, 3 parts of urea and 80 parts of clear water by weight.
5. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise 2 parts of the compound microorganism, 2.3 parts of molasses, 2.5 parts of glutamic acid, 4 parts of urea and 86 parts of clear water by weight.
6. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise 4 parts of the compound microorganism, 3.7 parts of molasses, 5 parts of glutamic acid, 1 part of urea and 91 parts of clean water by weight.
7. The microbial preparation of claim 1 or 2, wherein: the raw materials comprise, by weight, 5 parts of the compound microorganism, 5 parts of the molasses, 0.2 part of the glutamic acid, 5 parts of the urea and 95 parts of the clean water.
8. The microbial preparation of claim 1 or 2, wherein: the compound microorganism comprises verticillium dahliae, verticillium chlamydosporium, lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis.
9. Use of a microbial preparation according to any one of claims 1 to 8, wherein: uniformly spraying the microbial preparation on kitchen garbage of residents, sealing the kitchen garbage, and fermenting at the temperature of 20 ℃ for 30-40 days to obtain the biological organic fertilizer.
10. Use according to claim 9, characterized in that: the mass ratio of the microbial preparation to the kitchen garbage is 1:500-1: 50.
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Cited By (1)
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