CN112624854A - Microbial organic compound fertilizer for low-temperature environment and preparation method thereof - Google Patents
Microbial organic compound fertilizer for low-temperature environment and preparation method thereof Download PDFInfo
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- CN112624854A CN112624854A CN202011641009.2A CN202011641009A CN112624854A CN 112624854 A CN112624854 A CN 112624854A CN 202011641009 A CN202011641009 A CN 202011641009A CN 112624854 A CN112624854 A CN 112624854A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B13/00—Fertilisers produced by pyrogenic processes from phosphatic materials
- C05B13/02—Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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Abstract
The invention provides a microbial organic compound fertilizer for a low-temperature environment, which comprises a compound fertilizer component, an organic matter component and a microbial component, wherein the compound fertilizer component comprises nitrogen, phosphorus, potassium and trace element components, and the microbial component comprises a bactericidal microorganism and a heating microorganism; according to the invention, by using the bactericidal microorganism and the heating microorganism in a matching manner, normal decomposition of organic matters and proper propagation of the bactericidal microorganism in a low-temperature environment can be simultaneously ensured, so that nutrients required by plant growth are increased, inhibition on harmful germs is ensured, and the growth speed and health condition of plants in the low-temperature environment are greatly improved under the double effects.
Description
Technical Field
The invention relates to the technical field of microbial organic compound fertilizers, in particular to a microbial organic compound fertilizer for a low-temperature environment and a preparation method thereof.
Background
Chemical fertilizers lay a material foundation for the production and development of modern agriculture and the guarantee of food safety of human beings, but the low utilization rate of chemical fertilizers and the high environmental risk caused by the low utilization rate of chemical fertilizers are always great problems which bother the world. At present, due to the increase of the use amount of agriculture and chemical fertilizers all over the world, the dependence on pesticides and chemical fertilizers is increasingly strengthened, and a series of problems of insufficient use of organic fertilizers, imbalance of soil nutrient proportion, soil hardening, reduction of soil quality, river and underground water pollution and the like are caused. In order to avoid the 'pollution destruction before treatment' of the developed and serious countries and develop 'sustainable development' new agriculture, the use amount of pesticides and chemical fertilizers is reduced and effective substitutes are searched.
Therefore, all countries in the world actively explore methods and ways to improve fertilizer utilization and suppress environmental pollution, and in the last 20 years, the above objects are mainly achieved by changing nutrient release characteristics, improving fertilizer functions and the like. Firstly, the fertilizer efficiency is improved and the pollution is reduced through modification in a fertilizer nutrient release mode; secondly, the organic and inorganic fertilizers are compounded, so that the fertilizer efficiency is improved, and the pollution is reduced; and thirdly, by developing a formula fertilizer and improving the function of the fertilizer, the fertilizer efficiency is improved, and the pollution is reduced. Especially, research and development on the compounding of organic and inorganic fertilizers have become a current focus.
The microbial fertilizer has the characteristics of high fertilizer efficiency, no toxicity, no environmental pollution, low cost, energy conservation and the like, and is the most effective substitute of the fertilizer. The reasonable development and utilization of microbial fertilizers are important ways for the sustainable development of agriculture in China. The core of the microbial fertilizer is microorganisms and organic matters, so that the microbial fertilizer has the characteristics of microorganisms. The microbial resources are rich, the variety and the functions are various, and the fertilizer can be developed into fertilizers with different functions and different purposes. And the microbial strains can be artificially bred, continuously purified and rejuvenated to improve the activity of the strains, and particularly, with the further development of biotechnology, the acquisition of the required strains by a genetic engineering method becomes possible. The microbial fertilizer contains some secondary metabolites of microorganisms, wherein some of the secondary metabolites are growth hormones of crops, so that the growth and development of the crops can be stimulated in the seedling stage of the crops, and the quality of the crops can be improved in the mature stage of the crops; and some of the plants have the function of controlling pests and diseases. In addition, from the view of environmental resources, the microbial fertilizer has the characteristics of resource reutilization, no toxicity, no harm, no pollution and low cost.
However, the microbial compound fertilizer used at present has a certain problem, because the suitable propagation temperature of most of the microorganisms used for the compound fertilizer at present is above 15 ℃, the growth and propagation speed of the microorganisms in the fertilizer is very slow when the temperature is low in cold areas or winter, if the fertilizer is required to be applied at the moment, the required effects of sterilization, growth stimulation and the like of the added microorganisms are difficult to achieve, and actually, some harmful germs capable of growing and propagating under the low-temperature condition exist, so the microbial fertilizer suitable for being used under the low-temperature condition is required.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a microbial organic compound fertilizer for a low-temperature environment and a preparation method thereof, which solve the problem that the microbial growth and reproduction speed in the fertilizer is very low in the cold region or at a low temperature in winter, so that the effects of sterilization, growth stimulation and the like are difficult to achieve in the prior art.
The invention provides a microbial organic compound fertilizer for a low-temperature environment, which comprises a compound fertilizer component, an organic matter component and a microbial component, wherein the compound fertilizer component comprises nitrogen, phosphorus, potassium and trace element components, the microbial component comprises a bactericidal microorganism and a heating microorganism, and the weight percentages of the raw materials are as follows:
15 to 33 percent of nitrogen
12 to 31 percent of phosphorus
16 to 38 percent of potassium
17-28% of organic matters;
the rest is microbial components.
Further, the nitrogen component is urea; the phosphorus element is one or a mixture of two of calcium magnesium phosphate fertilizer and monoammonium phosphate; the potassium element is potassium sulfate.
Further, the organic matter is one or a mixture of more of wood chips, bran and straws.
Further, the bactericidal microorganism comprises one of bacillus subtilis, bacillus brevis and paenibacillus mucilaginosus.
Further, the heating microorganism includes one of Acetobacter pasteurianus, Pseudomonas stutzeri, and Pantoea agglomerans.
Further, the microbial component also comprises rhodospirillum morganii.
Preferably, the compound fertilizer comprises the following components (the content of microorganisms in unit weight) calculated by weight percentage:
urea 22-28%
13 to 24 percent of calcium magnesium phosphate fertilizer
23 to 29 percent of potassium sulfate
19-27% of bran;
the balance of the mixture of bacillus subtilis, brevibacillus laterosporus, pseudomonas stutzeri and pantoea agglomerans.
The invention also provides a preparation method of the microbial organic compound fertilizer for the low-temperature environment, which comprises the following steps:
(1) preparing the components of the compound fertilizer: after weighing the compound fertilizer raw materials in proportion, respectively scattering the raw materials, and then uniformly mixing and stirring the raw materials;
(2) preparing a microbial component: respectively fermenting and propagating the bactericidal microorganisms and the heating microorganisms by using culture solutions, then adding water for dilution, simultaneously weighing proper organic matters and crushing the organic matters into powder, adding the diluted microbial fermentation liquor into the organic matter powder so that the organic matters fully adsorb the microbial fermentation liquor, and finally drying the adsorbed organic matter powder at low temperature and scattering the organic matter powder into powder to obtain a microbial component;
(3) mixing: mixing the compound fertilizer components prepared in the steps (1) and (2) with the microbial components, and then fully stirring to obtain a powdery microbial organic compound fertilizer;
(4) and (3) granulation: and (3) granulating the powdery microbial organic compound fertilizer by using a granulator, and drying the obtained granular product at a low temperature to obtain a finished product.
Further, the low-temperature drying in the steps (2) and (4) is carried out for 30-45min at the temperature of 70-80 ℃ by using a drying furnace.
Further, in the step (2), the diluted fermentation liquor of the heated microorganism is mixed and stirred with the organic matter, and then the sterilized microorganism is added for adsorption mixing.
The technical principle of the invention is as follows: the microorganism components comprise a bactericidal microorganism and a heating microorganism, wherein the heating microorganism is a type with a higher propagation and decomposition rate in a cold-resistant microorganism, so that the relatively higher growth speed can be kept in a low-temperature environment of 0-10 ℃, organic matters are further decomposed in a large amount, heat is generated, the overall temperature of soil near the applied fertilizer is increased, the bactericidal microorganism mixed in the fertilizer can also accelerate the growth and the reproduction speed, the effects of accelerating the decomposition of the organic matters and promoting the growth of plants can be achieved, the growth of the bactericidal microorganism can be assisted, and the invasion of harmful germs can be inhibited.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, by using the bactericidal microorganism and the heating microorganism in a matching manner, normal decomposition of organic matters and proper propagation of the bactericidal microorganism in a low-temperature environment can be simultaneously ensured, so that nutrients required by plant growth are increased, inhibition on harmful germs is ensured, and the growth speed and health condition of plants in the low-temperature environment are greatly improved under the double effects;
2. according to the invention, the temperature of the fertilizer is increased by using the process of decomposing organic matters by using low-temperature-resistant microorganisms, so that another microorganism with a sterilization effect can reproduce to play a sterilization function, other inorganic chemical substances are not required to be introduced in the whole process, the final product cannot cause pollution, and the environment-friendly fertilizer has good environment-friendly performance;
3. according to the invention, rhodospirillum morganii is also introduced into the microbial components, and the rhodospirillum morganii is taken as a mixotrophic bacterium, and can grow in a micro-aerobic manner when being buried at the bottom of the ground and in a dark environment, so that organic matters are decomposed to generate heat in the early stage after fertilization, and the effects of heating and heat preservation are achieved; after a period of fertilization, part of rhodospirillum morganii is propagated to the surface of soil to be in contact with illumination, and photosynthesis can be carried out to generate oxygen and organic matters, so that oxygen and energy are further provided for other nearby bacteria, the growth speed of the bactericidal microorganisms and the heating microorganisms is increased, the growth speed of plants and microorganisms in a low-temperature environment is further increased, and a better basis is provided for preventing and controlling germs.
Detailed Description
The technical solution of the present invention is further illustrated by the following examples.
The inorganic fertilizer and the microbial strain used in the following examples of the present invention were purchased from the market, wherein urea was crystal urea produced by china agricultural resources group limited, calcium magnesium phosphate was produced by china billows sea chemical limited, potassium sulfate was produced by anhui friend tai bioengineering limited, and monoammonium phosphate was produced by north Hu Xingdong honest chemical limited.
Wherein the Bacillus subtilis is purchased from Shanghai biological Collection, CMCC (B)63501 standard strain; the brevibacillus laterosporus is purchased from CICC21185 standard strain of China agricultural microorganism strain collection center; the paenibacillus jelly is purchased from China agricultural microorganism strain collection center, ACCC10168 standard strain; rhodospirillum morganii was purchased from ACCC00323 standard strain of Shanghai biological Collection; acetobacter pasteurianus is a strain CGMCC No.17802 purchased from Shanghai Industrial microbiology institute, and Pseudomonas stutzeri is a strain ATCC17832 purchased from China general microbiological culture Collection center; the Pantoea agglomerans is CGMCC No.16174 strain purchased from Shanghai collection center.
Example 1:
the microbial organic compound fertilizer in the embodiment is prepared according to the following steps:
(1) preparing the components of the compound fertilizer: weighing 15% of urea, 30% of monoammonium phosphate and 38% of potassium sulfate according to weight percentage respectively, scattering the four, and then mixing and stirring uniformly.
(2) Preparing a microbial component: picking 1-2 ring thalli from bacillus subtilis preserved on a slant or frozen in glycerol, inoculating the thalli into a seed culture medium, and carrying out shake flask culture lOh at 28 ℃ and 250r/min to obtain a first-stage seed solution; inoculating the primary seed solution in a liquid amplification LB culture medium in an inoculation amount of 2-5%, and shake-culturing lOh at 28 ℃ and 180r/min to obtain a secondary seed solution; inoculating the second-stage seeds in a fermentation culture medium at an inoculation amount of 2% -5%. Fermenting for 24 hours in a fermentation tank at the temperature of 28 ℃ and the rotating speed of a stirring paddle of 180r/min until the end point to obtain bacillus subtilis fermentation liquor; then repeating the steps on the acetobacter pasteurianus to obtain a fermentation liquor of the acetobacter pasteurianus;
inoculating 5% (volume percentage below) Rhodospirillum mordii strain in triangular flask containing culture medium under aseptic condition, covering with aseptic rubber stopper, and culturing at 28 + -2 deg.C under natural or incandescent light for 2-3 days to obtain first-class strain; then inoculating 10% of the first-stage strain with a new container and a new culture medium, and culturing for 2-3 days to obtain a second-stage strain; inoculating 20% of the second-level strain with a new container and culture medium, and culturing for 2-3 days to obtain a third-level strain to obtain a Rhodospirillum mordii fermentation broth;
weighing 17% of bran by weight percent, crushing the bran into powder, diluting the Acetobacter pasteurianus fermentation liquid by using clear water by 10 times, adding the diluted product into organic matter powder to enable the organic matter to fully adsorb the microbial fermentation liquid, drying the adsorbed organic matter powder at 70 ℃ for 30min, scattering the organic matter powder into powder, finally adding mixed liquid of the bacillus subtilis fermentation liquid and the Rhodospirillum morganii fermentation liquid diluted by 10 times, stirring and mixing the mixed liquid after the powder fully absorbs the fermentation liquid, drying the mixed liquid at 70 ℃ for 30min, and scattering the mixed liquid into powder to obtain a mixed component of the microbes and the organic matter;
(3) mixing: mixing the two components prepared in the steps (1) and (2), and then fully stirring to obtain a powdery microbial organic compound fertilizer, wherein the actual total content of microbes is 0.3-0.7 hundred million/g;
(4) and (3) granulation: granulating the powdery microbial organic compound fertilizer by using a granulator, and drying the obtained granular product at 70 ℃ for 30 min; and finally, screening the dried granular microbial organic compound fertilizer to ensure that the granularity of the powdery microbial organic compound fertilizer is not more than 1.5mm and the granularity of the granular microbial organic compound fertilizer is 1.5-4.5 mm.
Example 2:
the microbial organic compound fertilizer in the embodiment is prepared according to the following steps:
(1) preparing the components of the compound fertilizer: 28 percent of urea, 31 percent of calcium magnesium phosphate fertilizer and 16 percent of potassium sulfate are respectively weighed according to the weight percentage, and the three are respectively scattered and then mixed and stirred evenly.
(2) Preparing a microbial component: selecting 1-2 ring thallus from Brevibacillus laterosporus preserved on a slant or frozen in glycerol, inoculating the thallus into a seed culture medium, and carrying out shake flask culture lOh at the temperature of 28 ℃ and the temperature of 150-; inoculating the primary seed solution in a liquid amplification LB culture medium in an inoculation amount of 2-5%, and shake-culturing lOh at 28 ℃ and 180r/min to obtain a secondary seed solution; inoculating the second-stage seeds in a fermentation culture medium at an inoculation amount of 2% -5%. Fermenting for 24 hours in a fermentation tank at the temperature of 28 ℃ and the rotating speed of a stirring paddle of 180r/min until the end point to obtain brevibacillus laterosporus fermentation liquor; then, the steps are repeated for the pseudomonas stutzeri to obtain fermentation liquor of the pseudomonas stutzeri;
inoculating 5% (volume percentage below) Rhodospirillum mordii strain in triangular flask containing culture medium under aseptic condition, covering with aseptic rubber stopper, and culturing at 28 + -2 deg.C under natural or incandescent light for 2-3 days to obtain first-class strain; then inoculating 10% of the first-stage strain with a new container and a new culture medium, and culturing for 2-3 days to obtain a second-stage strain; inoculating 20% of the second-level strain with a new container and culture medium, and culturing for 2-3 days to obtain a third-level strain to obtain a Rhodospirillum mordii fermentation broth;
weighing 25 wt% of sawdust, bran and straw, crushing the sawdust, bran and straw into powder, diluting pseudomonas stutzeri fermentation liquor by using clear water by 10 times, adding the diluted pseudomonas stutzeri fermentation liquor into organic matter powder to enable organic matter to fully adsorb the microbial fermentation liquor, drying the adsorbed organic matter powder at 70 ℃ for 30min, scattering the organic matter powder into powder, finally adding mixed liquid of bacillus laterosporus fermentation liquor and rhodospirillum morchelli fermentation liquor diluted by 10 times, stirring and mixing the mixed liquid after the powder fully absorbs the fermentation liquor, drying the mixed liquid at 70 ℃ for 30min, and scattering the mixed liquid into powder to obtain mixed components of the microbes and the organic matter;
(3) mixing: mixing the two components prepared in the steps (1) and (2), and then fully stirring to obtain a powdery microbial organic compound fertilizer, wherein the actual total content of microbes is 0.3-0.7 hundred million/g;
(4) and (3) granulation: granulating the powdery microbial organic compound fertilizer by using a granulator, and drying the obtained granular product at 75 ℃ for 38 min: and screening the dried granular microbial organic compound fertilizer to ensure that the granularity of the powdery microbial organic compound fertilizer is not more than 1.5mm and the granularity of the granular microbial organic compound fertilizer is 1.5-4.5 mm.
Example 3:
the microbial organic compound fertilizer in the embodiment is prepared according to the following steps:
(1) preparing the components of the compound fertilizer: 33 percent of urea, 23 percent of calcium magnesium phosphate fertilizer and 16 percent of potassium sulfate are weighed according to the weight percentage respectively, and the three are respectively scattered and then mixed and stirred evenly.
(2) Preparing a microbial component: picking 1-2 rings of bacteria from paenibacillus jelly preserved on a slant or frozen in glycerol, inoculating the bacteria into a seed culture medium, and performing shake-flask culture lOh at 28 ℃ and 250r/min to obtain a first-stage seed solution; inoculating the primary seed solution in a liquid amplification LB culture medium in an inoculation amount of 2-5%, and shake-culturing lOh at 28 ℃ and 180r/min to obtain a secondary seed solution; inoculating the second-stage seeds in a fermentation culture medium at an inoculation amount of 2% -5%. Fermenting for 24 hours in a fermentation tank at the temperature of 28 ℃ and the rotating speed of a stirring paddle of 180r/min until the end point to obtain the jelly-like bacillus amyloliquefaciens fermentation liquid; then repeating the steps on the Pantoea agglomerans to obtain fermentation liquor of the Pantoea agglomerans;
inoculating 5% (volume percentage below) Rhodospirillum mordii strain in triangular flask containing culture medium under aseptic condition, covering with aseptic rubber stopper, and culturing at 28 + -2 deg.C under natural or incandescent light for 2-3 days to obtain first-class strain; then inoculating 10% of the first-stage strain with a new container and a new culture medium, and culturing for 2-3 days to obtain a second-stage strain; inoculating 20% of the second-level strain with a new container and culture medium, and culturing for 2-3 days to obtain a third-level strain to obtain a Rhodospirillum mordii fermentation broth;
weighing 28% of sawdust and bran by weight percent, crushing the sawdust and the bran into powder, diluting the pantoea agglomerans fermentation broth by clear water by 10 times, adding the diluted pantoea agglomerans fermentation broth into organic matter powder to enable organic matter to fully adsorb the microbial fermentation broth, drying the adsorbed organic matter powder at 70 ℃ for 30min, breaking the organic matter powder into powder, finally adding mixed liquid of the jellylike bacillus mucilaginosus fermentation broth and the rhodospirillum morganii fermentation broth diluted by 10 times, stirring and mixing the powder after the powder fully absorbs the fermentation broth, drying the mixed liquid at 70 ℃ for 30min, breaking the mixed liquid into powder to obtain mixed components of the microorganisms and the organic matter;
(3) mixing: mixing the two components prepared in the steps (1) and (2), and then fully stirring to obtain a powdery microbial organic compound fertilizer, wherein the actual total content of microbes is 0.3-0.7 hundred million/g;
(4) and (3) granulation: granulating the powdery microbial organic compound fertilizer by using a granulator, and drying the obtained granular product at 80 ℃ for 45 min; and screening the dried granular microbial organic compound fertilizer to ensure that the granularity of the powdery microbial organic compound fertilizer is not more than 1.5mm and the granularity of the granular microbial organic compound fertilizer is 1.5-4.5 mm.
Two sets of comparative examples, example 4 and example 5, were also used in the present invention, wherein example 4 is a microbial organic fertilizer from north Heibei Devoro Multi Fertilizer, Inc., and example 5 is a common NPK compound fertilizer from Strobil.
Under the same conditions as in examples 1 to 5, field tests were carried out, wherein the test site was a certain field in the north of china, the temperature during the tests was 1-8 ℃, the acre yield of pakchoi was used as the test target of the fertilizer effect, and the obtained test results are shown in table 1:
| example 1 | Example 2 | Example 3 | Examples4 | Example 5 | |
| Yield (kg/mu) | 1825 | 1813 | 1846 | 1478 | 1282 |
Table 1: yield per mu of pakchoi under different fertilizers
As can be seen from the table 1, the microbial fertilizer purchased in the market has a certain improvement on the per mu yield compared with the common compound fertilizer, but because the temperature is low, the microorganisms are difficult to grow and reproduce fully, the added microorganisms cannot play the effects of full sterilization, disease resistance and the like, and the yield increasing effect is not good enough; the three embodiments of the invention have obvious yield per mu improvement compared with the two embodiments of the control group, which fully shows that the function of enhancing the growth and reproduction speed of the sterilization microorganism in a low-temperature environment by heating the microorganism has obvious improvement effect on the yield of crops.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (10)
1. A microbial organic compound fertilizer for low-temperature environment is characterized in that: the composite fertilizer comprises a composite fertilizer component, an organic matter component and a microorganism component, wherein the composite fertilizer component comprises nitrogen, phosphorus, potassium and trace element components, the microorganism component comprises a bactericidal microorganism and a heating microorganism, and the weight percentages of the raw materials are as follows:
15 to 33 percent of nitrogen
12 to 31 percent of phosphorus
16 to 38 percent of potassium
17-28% of organic matters;
the rest is microbial components.
2. The microbial organic compound fertilizer for low-temperature environment according to claim 1, wherein: the nitrogen is composed of urea; the phosphorus element is one or a mixture of two of calcium magnesium phosphate fertilizer and monoammonium phosphate; the potassium element is potassium sulfate.
3. The microbial organic compound fertilizer for low-temperature environment according to claim 2, wherein: the organic matter is one or a mixture of more of sawdust, bran and straw.
4. A microbial organic compound fertilizer for use in a low temperature environment according to claim 3, wherein: the bactericidal microorganism comprises one of bacillus subtilis, bacillus brevis and paenibacillus mucilaginosus.
5. The microbial organic compound fertilizer for low-temperature environment according to claim 4, wherein: the heating microorganism comprises one of Acetobacter pasteurianus, Pseudomonas stutzeri and Pantoea agglomerans.
6. The microbial organic compound fertilizer for low-temperature environment according to claim 5, wherein: the microbial component further comprises rhodospirillum morganii.
7. The microbial organic compound fertilizer for low-temperature environment according to claim 6, wherein: the compound fertilizer comprises the following components (the content of microorganisms in unit weight) calculated by weight percentage:
urea 22-28%
13 to 24 percent of calcium magnesium phosphate fertilizer
23 to 29 percent of potassium sulfate
19-27% of bran;
the balance of the mixture of bacillus subtilis, brevibacillus laterosporus, pseudomonas stutzeri and pantoea agglomerans.
8. A method for preparing a microbial organic compound fertilizer for a low temperature environment according to claim 1, comprising the steps of:
(1) preparing the components of the compound fertilizer: after weighing the compound fertilizer raw materials in proportion, respectively scattering the raw materials, and then uniformly mixing and stirring the raw materials;
(2) preparing a microbial component: respectively fermenting and propagating the bactericidal microorganisms and the heating microorganisms by using culture solutions, then adding water for dilution, simultaneously weighing proper organic matters and crushing the organic matters into powder, adding the diluted microbial fermentation liquor into the organic matter powder so that the organic matters fully adsorb the microbial fermentation liquor, and finally drying the adsorbed organic matter powder at low temperature and scattering the organic matter powder into powder to obtain a microbial component;
(3) mixing: mixing the compound fertilizer components prepared in the steps (1) and (2) with the microbial components, and then fully stirring to obtain a powdery microbial organic compound fertilizer;
(4) and (3) granulation: and (3) granulating the powdery microbial organic compound fertilizer by using a granulator, and drying the obtained granular product at a low temperature to obtain a finished product.
9. The method for preparing a microbial organic compound fertilizer for a low temperature environment according to claim 8, wherein: the low-temperature drying in the steps (2) and (4) is carried out for 30-45min at the temperature of 70-80 ℃.
10. The method for preparing a microbial organic compound fertilizer for a low temperature environment according to claim 8, wherein: and (2) mixing and stirring the diluted fermentation liquor of the heated microorganisms and the organic matters, and then adding the sterilized microorganisms for adsorption mixing.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372425A (en) * | 2008-10-10 | 2009-02-25 | 黑龙江志远生物工程有限公司 | Microbial fertilizer and preparation thereof |
| CN102001870A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Inorganic-organic microbial compound fertilizer and preparation method thereof |
| CN102433262A (en) * | 2011-12-13 | 2012-05-02 | 新疆农业科学院微生物应用研究所 | Complex microbial agent for low-temperature methane fermentation and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372425A (en) * | 2008-10-10 | 2009-02-25 | 黑龙江志远生物工程有限公司 | Microbial fertilizer and preparation thereof |
| CN102001870A (en) * | 2010-11-11 | 2011-04-06 | 唐清池 | Inorganic-organic microbial compound fertilizer and preparation method thereof |
| CN102433262A (en) * | 2011-12-13 | 2012-05-02 | 新疆农业科学院微生物应用研究所 | Complex microbial agent for low-temperature methane fermentation and preparation method thereof |
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