CN112616561A - Preparation method of mushroom liquid mushroom stick and method for culturing mushrooms by using mushroom stick - Google Patents
Preparation method of mushroom liquid mushroom stick and method for culturing mushrooms by using mushroom stick Download PDFInfo
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- CN112616561A CN112616561A CN201910902534.6A CN201910902534A CN112616561A CN 112616561 A CN112616561 A CN 112616561A CN 201910902534 A CN201910902534 A CN 201910902534A CN 112616561 A CN112616561 A CN 112616561A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention provides a preparation method of a mushroom liquid stick, which comprises the following steps: weighing, pre-wetting, mixing, bagging, pricking, sterilizing and taking out of a pot, wherein the method for culturing the lentinus edodes by using the mushroom stick comprises the following steps: the method comprises the steps of inoculation, spore culture, outer bag removal, pile turning, twice oxygenation, color conversion culture and fruiting, the survival rate of strains is high after the mushroom sticks are inoculated, the growth speed of mushroom hyphae cultured by the method is high, the yield is high, the fruiting quality and the yield of the mushrooms are good, mushroom buds are strong during fruiting, the problem of outbreak fruiting is solved, and the fruiting duration is strong and the quality is excellent.
Description
Technical Field
The invention relates to the technical field of mushroom culture, in particular to a preparation method of a mushroom liquid mushroom stick and a method for culturing mushrooms by using the mushroom liquid mushroom stick.
Background
Lentinus edodes, also known as Lentinus edodes and Lentinus edodes, is the fruiting body of Lentinus edodes of Pleurotaceae, and has high nutritive value. The mushroom is the second most edible mushroom in the world and is one of the special products in China, and is called mountain delicacies among people.
The protein content of the mushroom is up to 18.6 percent, the fat content is as low as 4.8 percent, the carbohydrate content is 71 percent, the crude fiber content is 9.6 percent, and the mushroom.
Lentinus Edodes has various biological activities and pharmacological effects. The mushroom is sweet in taste and neutral in nature, mainly treats anorexia and hypodynamia, and is a high-protein low-fat nutritional health food. The ergosterol content in the mushrooms is very high, and the shiitake powder is effective in preventing and treating rickets; lentinan (beta-1, 3 glucan) can enhance the cellular immunity, thereby inhibiting the growth of cancer cells; the shiitake mushroom contains more than 40 enzymes of six enzymes, and can correct enzyme deficiency of human body; the fat in the mushroom contains fatty acid, which is beneficial to reducing blood fat of human body.
The traditional mushroom production generally adopts solid strains for inoculation culture, the strains mainly comprise sawdust strains, branch strains and other conventional solid strains, the production period of the method is long, the method generally needs 55-60 days, the process is complex, and the production method has low efficiency; the production process of the strain needs a large space, needs a large amount of manpower and material resources, and has high cost; the culture period of the hyphae is long, the difference of the hyphae age in the culture medium is large, when the front end hyphae are in a budding state, the hyphae on the surface of the substrate are nearly aged, the fruiting of the cultivated species is irregular, the scale production of the mushrooms cannot be achieved, and the production requirements of people cannot be met. Instead, the liquid culture technology is adopted for production, the liquid culture has the characteristics of short production period, fast hypha growth, good hypha dispersibility, multiple germination points, capability of obtaining a large amount of mycelium and metabolites in a short time, suitability for industrial production and the like, can utilize the mycelium and fermentation liquor to produce health care products and extract effective components, and is a brand-new way for developing the production of the shiitake mushrooms.
However, in the process of producing mushrooms by liquid fermentation, the most important is whether the formula of the used liquid culture medium is good or not and whether the culture conditions are proper or not, which directly determines the growth of mushroom mycelia and the formation of metabolites, the survival rate of the mushroom liquid strains after being inoculated with mushroom sticks is low, the phenomenon of dead seeds often occurs, the survival rate is unstable, the mushroom sticks are soft and elastic after the mycelia are overgrown, the mushroom buds are weak and the quantity is large in the early stage of fruiting, and the later stage of fruiting is weak.
Disclosure of Invention
The invention aims to solve the problems that the survival rate of mushroom liquid strains inoculated with mushroom sticks is low, the mushroom sticks are frequently killed, the survival rate is unstable, the mushroom sticks are soft and inelastic after hyphae grow over, and mushroom buds are thin and weak in quantity and are large in quantity in the early fruiting period and weak in later fruiting period after the mushroom sticks are mature in the prior art.
The technical scheme provided by the invention is as follows:
a preparation method of a mushroom liquid stick comprises the following steps:
step 1: weighing
Weighing the following components in percentage by weight: the composite material comprises wood dust, wheat bran, gypsum and calcium superphosphate, wherein the mass parts of the components are as follows: 78 parts of wood chips, 20 parts of wheat bran, 1 part of gypsum and 1 part of calcium superphosphate.
Step 2: pre-wetting
Pre-wetting all the selected sawdust in advance.
And step 3: mixing material
The wheat bran and the gypsum are stirred uniformly, the wood chips are put into a stirring hopper, water is added while stirring, and the mixture is stirred uniformly for 15 to 25 minutes, so that the water content of the final culture material is 58 percent, and the pH value is 6 +/-0.5.
And 4, step 4: bagging-off
The production fungus bags are taken and filled into the culture materials.
And 5: pricking hole
And (4) punching holes on the fungus bags by a hole punching person according to the fungus inoculation punching position, adhering paper adhesive tapes with proper sizes, and flatly and tightly adhering to obtain the fungus sticks.
Step 6: sterilization
And (4) placing the fungus sticks obtained in the step (5) into a sterilization pot for high-pressure sterilization.
And 7: taking out of the pot
Taking out the fungus sticks, cooling to 60 ℃, loading into a pot car, and conveying to an inoculation room.
Preferably, the pre-wetting time in step 2 is more than 12 hours, so as to break off the large wood chips, and the central moisture of the large wood chips is uniform.
Preferably, the specific operations in step 4 are as follows: sleeving the fungus bags on a machine, keeping the bag openings clean without sticking materials, filling the culture materials with the height of 40cm +/-1, and keeping the weight of a single fungus stick not lower than 2.05-2.20 Kg.
Preferably, the sterilization parameters set by the sterilization pot (sterilization pot model) in the step 5 are as follows: vacuumizing for 2 times, wherein the first time is-0.055 MPa, the second time is-0.05 MPa, and the steam supplementing pressure is-0.02 MPa;
primary heat preservation: the temperature is 105 ℃, the time is 30 minutes, the pressure is 0.050MPa, the temperature rise and steam exhaust period is 1, 10/40 is realized, and the steam exhaust switching temperature is 102 ℃;
secondary heat preservation: the temperature is 110 ℃, the time is 30 minutes, the pressure is 0.085MPa, and the heating and steam discharging period is 2: 6.0/40;
and (3) sterilization stroke: the temperature is 120 ℃, the time is 420 minutes, the pressure is 0.100MPa, and the steam exhaust period is 2.0/30;
closing and placing stroke: time 80 minutes, exhaust period 0/0.
Preferably, in the step 7, the pot discharging vehicle is cleaned before discharging, a pot door in the direction of a production workshop must be closed when discharging, the fungus sticks with the temperature reduced to 60 ℃ are loaded into the pot discharging vehicle, and the loaded fungus sticks and the sterilized tarpaulin are conveyed to an inoculation room.
Preferably, the wood chips are produced by selecting fresh mixed wood chips without mildew and with the water content of less than 40 percent according to requirements; selecting fresh, large and mildew-free wheat bran; the gypsum and the calcium superphosphate are selected from specified edible fungus production materials,
the method for culturing the shiitake mushrooms by using the shiitake mushroom liquid fungus sticks is characterized by comprising the following steps of:
step 1: inoculation of
And (3) reserving at least 20cm of operation ventilation channels between each row of the fungus sticks in the inoculation room, and inoculating the cultured liquid strains on the fungus sticks by using an inoculating machine under an aseptic condition when the fungus sticks are cooled to 25 ℃.
Step 2: spore culture
And (3) transporting the inoculated bacteria sticks to a culture room for culture, wherein the bacteria culture environment is as follows:
(1) temperature: 24 ℃;
(2) air humidity: 30% +/-10%;
(3) the illumination is less than 50Lx before 90% of fungus stick hyphae are fully grown in the bag;
(3) the carbon dioxide concentration is less than 3000ppm to ensure the air to be fresh.
And step 3: removing outer bag and turning over pile
And when 80% of the fungus sticks reach the growth diameter of the fungus ring of 6-8cm, removing the outer bag and turning, wherein the standard fungus sticks are regularly arranged in a triangular stack in the turning process, and an air channel with the length of not less than 10cm x 20cm is reserved between the stacks.
And 4, step 4: oxygen increasing device
First oxygenation: when 80% of fungus stick fungus circle grows two thirds, oxygenation is carried out by using a small gas aerator, oxygenation is carried out by taking the inoculation hole as the center of the small gas aerator, the fungus stick inoculation openings which complete oxygenation are outwards placed, are placed in order in a triangular stack and are placed according to the original gap.
And (3) oxygenation for the second time: when the bacteria nodule is over 30%, the bacteria stick is added with second oxygen, the oxygen is added by an oxygen adding machine, the total hole number is between 35 and 55, the bacteria stick bacteria receiving port after oxygen adding is upward, the bacteria stick bacteria receiving port is orderly arranged in a triangular stack, and the bacteria stick bacteria receiving port is arranged according to a gap not smaller than the original gap.
And 5: color-changing culture
(1) Temperature: 22-24 ℃;
(2) air humidity: 60% +/-10%;
(3) illumination: 100-200 lux to avoid direct light;
(4) concentration of carbon dioxide: less than 3000 ppm.
Step 6: fruiting body
The color of the fungus stick is more than 85% of that of the fungus bag, the fungus stick is dark brown and has white spots, and the weight standard is as follows: the weight of the fungus stick is more than 1.55Kg after the fungus is removed from the bag in the fungus culturing chamber. Primordium distribution standard: the primordium of the fungus stick is uniformly distributed. Preferably, before the inoculation in step 1, the color stripe cloth cleaned by potassium permanganate is prepared in advance in an inoculation room, a dust collector is used for carrying out secondary dust removal treatment on a field in the inoculation room, 3/1000 potassium permanganate solution is used for disinfection, the color stripe cloth is paved on the ground, and 3/1000 potassium permanganate solution is used for secondary disinfection on the color stripe cloth.
The invention has the following beneficial effects:
(1) the cultivation medium filled in the mushroom stick is reasonably mixed with sawdust, wheat bran, gypsum and calcium superphosphate, proper humidity and pH value are adjusted, the survival rate of the mushroom is high after the mushroom stick is inoculated, and the growth speed of the mushroom hyphae cultivated and cultivated is high and the yield is high.
(2) The obtained mushroom sticks are subjected to a strict high-pressure sterilization process, and in the inoculation process, an inoculation room is strictly disinfected, so that the mixed mushroom pollution is prevented, the defect of poor resistance of the shiitake mushrooms is overcome, and the shiitake mushroom fruiting quality is good and the yield is high.
(3) Adopting proper conditions for mycelium culture, and carrying out oxygen enrichment twice in a fruiting period, wherein the oxygen enrichment for the first time is as follows: and (3) when 80% of fungus sticks and fungus circles grow two thirds, carrying out secondary oxygenation: when the bacterium tumor is more than 30%, the bacterium rod increases oxygen for the second time, and the special oxygen increasing time can enhance the growth activity and the metabolic capability of the mycelium and promote the decomposition, transformation, absorption and utilization of the mycelium on nutrient substances.
(4) The fungus stick cultured by the invention has strong fungus buds when growing the mushrooms, and solves the problem of outbreak of the mushrooms.
(5) The moisture is changed quickly after water injection, the fruiting continuous force is strong, and the quality is excellent.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
A preparation method of a mushroom liquid stick comprises the following steps:
step 1: weighing
The following components were weighed: wood dust, wheat bran, gypsum and calcium superphosphate, wherein the components are weighed according to the mass respectively: 78kg of wood chips, 20kg of wheat bran, 1kg of gypsum and 1kg of calcium superphosphate, wherein the wood chips are produced according to requirements, fresh and mildew-free miscellaneous wood chips with water content not more than 40%; selecting fresh, large and mildew-free wheat bran; the gypsum and the calcium superphosphate are selected from specified edible fungus production materials.
Step 2: pre-wetting
Pre-wetting all the selected sawdust in advance for more than 12 hours to break off large sawdust blocks, wherein the central moisture of the sawdust blocks is uniform.
And step 3: mixing material
Uniformly stirring wheat bran and gypsum, adding sawdust into a stirring hopper, adding water while stirring, uniformly stirring for 15-25 minutes to make the water content of the final culture material be 58% and the pH value be 6 +/-0.5, and detecting by using a commercially available professional moisture tester and a pH value tester.
And 4, step 4: bagging-off
The production fungus bags are taken and filled into the culture materials.
Personnel configuration: every five people are a set of, and alone cover the bag, alone connect the bag, alone prick the bag, alone pierce through sticky tape, alone inspect micropore dress basket, cover the bag: the method comprises the following steps of (1) adopting a single-bag bagging mode, taking a production fungus bag, sleeving the fungus bag on a machine, wherein the bagging speed is matched with bag bundling personnel, the bag opening is kept clean and is not stained, the height of a packaged culture material is 40cm +/-1, and the weight of a single fungus stick is not lower than 2.05-2.20 Kg; connect the bag personnel in time to connect the bag, the compaction charge level, the clearance sack, give and prick the bag personnel, prick the bag personnel and use special tying machine to hug closely the charge level and prick the mouth, prick the hole personnel and prick the hole according to connecing the fungus position of punching, glue the paper sticky tape that the size is fit for, paste according to flatly, give inspection micropore personnel, inspection micropore personnel carefully inspect the micropore, and will have the millipore fungus stick to choose out, glue the micropore with the sticky tape, accomplish not have the micropore, carry out the micropore inspection once more with the fungus bag that will handle, the frame is put to in good order to qualified fungus bag.
And 5: pricking hole
And (4) punching holes on the fungus bags by a hole punching person according to the fungus inoculation punching position, adhering paper adhesive tapes with proper sizes, and flatly and tightly adhering to obtain the fungus sticks.
Step 6: sterilization
Placing the fungus sticks obtained in the step (5) into a sterilization pot for high-pressure sterilization, and checking whether the sterilization pot is clean and whether the adhesive tape of the sterilization pot door is sealed before the fungus sticks are placed into the pot; boiler pressure table, sterilization pot thermometer, boiler water pump, whether the motor operation condition is normal, the staffing personnel use per 5 baskets as a set of, it puts to reach the alignment of four corners in the sterilization pot, record the panne quantity faithfully, and guarantee that the panne quantity is unanimous with each group fungus stick production total number, notice the loading, the transportation, the panne in-process should be taken slightly to put, avoid the fungus stick because of collision each other, the friction causes the pollution loss, close the front and back pot door according to closing the pot door flow by the sterilization personnel, the sterilization parameter that the sterilization pot set up is: vacuumizing for 2 times, wherein the first time is-0.055 MPa, the second time is-0.05 MPa, and the steam supplementing pressure is-0.02 MPa;
primary heat preservation: the temperature is 105 ℃, the time is 30 minutes, the pressure is 0.050MPa, the temperature rise and steam exhaust period is 1, 10/40 is realized, and the steam exhaust switching temperature is 102 ℃;
secondary heat preservation: the temperature is 110 ℃, the time is 30 minutes, the pressure is 0.085MPa, and the heating and steam discharging period is 2: 6.0/40;
and (3) sterilization stroke: the temperature is 120 ℃, the time is 420 minutes, the pressure is 0.100MPa, and the steam exhaust period is 2.0/30;
closing and placing stroke: time 80 minutes, exhaust period 0/0.
And 7: taking out of the pot
Taking out the fungus sticks, cooling to 60 ℃, loading into a pot car, and conveying to an inoculation room.
Preferably, the pre-wetting time in step 2 is more than 12 hours, so as to break off the large wood chips, and the central moisture of the large wood chips is uniform. And (3) cleaning the pot discharging vehicle before discharging, closing a pot door in the direction of a production workshop when discharging, loading the fungus sticks with the temperature reduced to 60 ℃ into the pot discharging vehicle, and conveying the loaded fungus sticks and the sterilized tarpaulin to an inoculation room.
The method for culturing the shiitake mushrooms by using the shiitake mushroom liquid fungus sticks is characterized by comprising the following steps of:
step 1: inoculation of
Before the inoculation, prepare in advance between the inoculation and use the clear color stripe cloth of potassium permanganate sanitization to carry out secondary dust removal treatment to the place between the inoculation with the dust catcher, use 3/1000 potassium permanganate solution to disinfect, pave color stripe cloth on ground again, use 3/1000 potassium permanganate solution to carry out secondary disinfection to color stripe cloth.
And (3) reserving at least 20cm of operation ventilation channels between each row of the fungus sticks in the inoculation room, and inoculating the cultured liquid strains on the fungus sticks by using an inoculating machine under an aseptic condition when the fungus sticks are cooled to 25 ℃.
Step 2: spore culture
And (3) transporting the inoculated bacteria sticks to a culture room for culture, wherein the bacteria culture environment is as follows:
(1) temperature: 24 ℃;
(2) air humidity: 30% +/-10%;
(3) the illumination is less than 50Lx before 90% of fungus stick hyphae are fully grown in the bag;
(3) the carbon dioxide concentration is less than 3000ppm to ensure the air to be fresh.
And step 3: removing outer bag and turning over pile
And when 80% of the fungus sticks reach the growth diameter of the fungus ring of 6-8cm, removing the outer bag and turning, wherein the standard fungus sticks are regularly arranged in a triangular stack in the turning process, and an air channel with the length of not less than 10cm x 20cm is reserved between the stacks.
And 4, step 4: oxygen increasing device
First oxygenation: when 80% of fungus stick fungus circle grows two thirds, oxygenation is carried out by using a small gas aerator, oxygenation is carried out by taking the inoculation hole as the center of the small gas aerator, the fungus stick inoculation openings which complete oxygenation are outwards placed, are placed in order in a triangular stack and are placed according to the original gap.
And (3) oxygenation for the second time: when the bacteria nodule is over 30%, the bacteria stick is added with second oxygen, the oxygen is added by an oxygen adding machine, the total hole number is between 35 and 55, the bacteria stick bacteria receiving port after oxygen adding is upward, the bacteria stick bacteria receiving port is orderly arranged in a triangular stack, and the bacteria stick bacteria receiving port is arranged according to a gap not smaller than the original gap.
And 5: color-changing culture
(1) Temperature: 22-24 ℃;
(2) air humidity: 60% +/-10%;
(3) illumination: 100-200 lux to avoid direct light;
(4) concentration of carbon dioxide: less than 3000 ppm.
Step 6: fruiting body
The color of the fungus stick is more than 85% of that of the fungus bag, the fungus stick is dark brown and has white spots, and the weight standard is as follows: the weight of the fungus stick is more than 1.55Kg after the fungus is removed from the bag in the fungus culturing chamber. Primordium distribution standard: the primordium of the fungus stick is uniformly distributed.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. The invention is therefore not to be limited to the specific details described herein, without departing from the general concept as defined by the appended claims and their equivalents.
Claims (8)
1. The preparation method of the liquid mushroom sticks is characterized by comprising the following steps of:
step 1: weighing
Weighing the following components in percentage by weight: the composite material comprises wood dust, wheat bran, gypsum and calcium superphosphate, wherein the mass parts of the components are as follows: 78 parts of wood chips, 20 parts of wheat bran, 1 part of gypsum and 1 part of calcium superphosphate;
step 2: pre-wetting
Pre-wetting all the selected sawdust in advance;
and step 3: mixing material
Uniformly stirring wheat bran and gypsum, adding sawdust into a stirring hopper, adding water while stirring, and uniformly stirring for 15-25 minutes to ensure that the water content of the final culture material is 58% and the pH value is 6 +/-0.5;
and 4, step 4: bagging-off
Taking a production fungus bag, and filling the production fungus bag into a culture material;
and 5: pricking hole
Punching holes on the fungus bags by a punching person according to the fungus inoculation punching position, adhering paper adhesive tapes with proper sizes, and tightly adhering according to the flat state to obtain fungus sticks;
step 6: sterilization
Placing the fungus sticks obtained in the step 5 into a sterilization pot for high-pressure sterilization;
and 7: taking out of the pot
Taking out the fungus sticks, cooling to 60 ℃, loading into a pot car, and conveying to an inoculation room.
2. The method for preparing liquid mushroom sticks of shiitake mushrooms according to claim 1, wherein the pre-wetting time in step 2 is more than 12 hours, based on breaking off large pieces of wood chips and uniform moisture in the center thereof.
3. The preparation method of the liquid mushroom stick of mushroom according to claim 1, characterized in that the specific operations in step 4 are as follows: sleeving the fungus bags on a machine, keeping the bag openings clean without sticking materials, filling the culture materials with the height of 40cm +/-1, and keeping the weight of a single fungus stick not lower than 2.05-2.20 Kg.
4. The method for preparing the liquid mushroom sticks of the lentinus edodes as claimed in claim 1, wherein the sterilization pot in the step 5 is set with the sterilization parameters as follows: vacuumizing for 2 times, wherein the first time is-0.055 MPa, the second time is-0.05 MPa, and the steam supplementing pressure is-0.02 MPa;
primary heat preservation: the temperature is 105 ℃, the time is 30 minutes, the pressure is 0.050MPa, the temperature rise and steam exhaust period is 1, 10/40 is realized, and the steam exhaust switching temperature is 102 ℃;
secondary heat preservation: the temperature is 110 ℃, the time is 30 minutes, the pressure is 0.085MPa, and the heating and steam discharging period is 2: 6.0/40;
and (3) sterilization stroke: the temperature is 120 ℃, the time is 420 minutes, the pressure is 0.100MPa, and the steam exhaust period is 2.0/30;
closing and placing stroke: time 80 minutes, exhaust period 0/0.
5. The method for preparing mushroom liquid sticks according to claim 1, wherein in the step 7, a pot discharging vehicle is cleaned before discharging, a pot door in the direction of a production workshop must be closed when discharging, the mushroom sticks with the temperature reduced to 60 ℃ are loaded into the pot discharging vehicle, and the loaded mushroom sticks and the sterilized tarpaulin are conveyed to an inoculation room.
6. The method for preparing liquid mushroom sticks of shiitake mushrooms according to claim 1, wherein the wood chips are miscellaneous wood chips which are fresh, do not have mildew and have a water content of not more than 40% and are produced according to requirements; selecting fresh, large and mildew-free wheat bran; the gypsum and the calcium superphosphate are selected from specified edible fungus production materials.
7. A method for cultivating shiitake mushroom by using the liquid stick of shiitake mushroom as claimed in any one of claims 1 to 6, comprising the steps of:
step 1: inoculation of
At least 20cm of operation ventilation channel is reserved between each row of the fungus sticks in the inoculation room, when the fungus sticks are cooled to 25 ℃, the cultured liquid strains are inoculated on the fungus sticks by using an inoculation machine under the aseptic condition;
step 2: spore culture
And (3) transporting the inoculated bacteria sticks to a culture room for culture, wherein the bacteria culture environment is as follows:
(1) temperature: 24 ℃;
(2) air humidity: 30% +/-10%;
(3) the illumination is less than 50Lx before 90% of fungus stick hyphae are fully grown in the bag;
(3) the carbon dioxide concentration is less than 3000ppm to ensure the air to be fresh;
and step 3: removing outer bag and turning over pile
When 80% of the fungus sticks reach the growth diameter of the fungus ring of 6-8cm, removing the outer bag and turning, wherein the standard fungus sticks are regularly arranged in a triangular pile during turning, and are arranged in rows, and an air channel with the length of not less than 10cm x 20cm is reserved between the piles;
and 4, step 4: oxygen increasing device
First oxygenation: when 80% of fungus stick fungus rings grow two thirds, a small gas aerator is used for aeration, an inoculation hole is used as the center of the small gas aerator for aeration, fungus stick inoculation openings which finish aeration are outwards arranged, are orderly arranged in a triangular stack and are arranged according to the original gap;
and (3) oxygenation for the second time: adding secondary oxygen when the bacteria tumors exceed 30%, and adding oxygen by using an oxygen adding machine, wherein the total number of holes is 35-55, the bacteria receiving openings of the bacteria rods after oxygen adding are upward, and the bacteria rods are orderly arranged in a triangular stack and are arranged according to the interval not smaller than the original interval;
and 5: color-changing culture
(1) Temperature: 22-24 ℃;
(2) air humidity: 60% +/-10%;
(3) illumination: 100-200 lux to avoid direct light;
(4) concentration of carbon dioxide: less than 3000 ppm;
step 6: fruiting body
The color of the fungus stick is more than 85% of that of the fungus bag, the fungus stick is dark brown and has white spots, and the weight standard is as follows: the weight of the fungus stick is more than 1.55Kg after the fungus is removed from the bag in the fungus culturing room, and the primordium distribution standard is as follows: the primordium of the fungus stick is uniformly distributed.
8. The method for cultivating mushrooms through the liquid mushroom sticks of mushrooms as claimed in claim 7, wherein, before the inoculation in step 1, the color stripe cloth cleaned with potassium permanganate is prepared in advance in the inoculation room, a dust collector is used for carrying out secondary dust removal treatment on the field in the inoculation room, 3/1000 potassium permanganate solution is used for disinfection, then the color stripe cloth is laid on the ground, and 3/1000 potassium permanganate solution is used for secondary disinfection on the color stripe cloth.
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