CN112592393A - 水稻早衰控制基因es1-3及其应用 - Google Patents
水稻早衰控制基因es1-3及其应用 Download PDFInfo
- Publication number
- CN112592393A CN112592393A CN202011626237.2A CN202011626237A CN112592393A CN 112592393 A CN112592393 A CN 112592393A CN 202011626237 A CN202011626237 A CN 202011626237A CN 112592393 A CN112592393 A CN 112592393A
- Authority
- CN
- China
- Prior art keywords
- ser
- leu
- glu
- asp
- pro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 85
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 80
- 235000009566 rice Nutrition 0.000 title claims abstract description 79
- 230000002028 premature Effects 0.000 title claims abstract description 37
- 240000007594 Oryza sativa Species 0.000 title description 7
- 241000209094 Oryza Species 0.000 claims abstract description 79
- 230000012010 growth Effects 0.000 claims abstract description 24
- 230000001105 regulatory effect Effects 0.000 claims abstract description 9
- 230000009758 senescence Effects 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 230000002829 reductive effect Effects 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 238000004383 yellowing Methods 0.000 claims description 7
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 3
- 238000012271 agricultural production Methods 0.000 claims description 2
- 238000012214 genetic breeding Methods 0.000 claims description 2
- 230000002068 genetic effect Effects 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 41
- 238000000034 method Methods 0.000 abstract description 18
- 238000010367 cloning Methods 0.000 abstract description 10
- 230000009261 transgenic effect Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000001276 controlling effect Effects 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 4
- 230000033228 biological regulation Effects 0.000 abstract description 3
- 238000010353 genetic engineering Methods 0.000 abstract description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 26
- 235000019804 chlorophyll Nutrition 0.000 description 23
- 229930002875 chlorophyll Natural products 0.000 description 22
- 108020004414 DNA Proteins 0.000 description 13
- 230000014634 leaf senescence Effects 0.000 description 12
- 230000000243 photosynthetic effect Effects 0.000 description 11
- 108010038633 aspartylglutamate Proteins 0.000 description 10
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 8
- 239000005977 Ethylene Substances 0.000 description 8
- 108010050848 glycylleucine Proteins 0.000 description 8
- 150000001413 amino acids Chemical group 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- XVZCXCTYGHPNEM-UHFFFAOYSA-N Leu-Leu-Pro Natural products CC(C)CC(N)C(=O)NC(CC(C)C)C(=O)N1CCCC1C(O)=O XVZCXCTYGHPNEM-UHFFFAOYSA-N 0.000 description 6
- 108010079364 N-glycylalanine Proteins 0.000 description 6
- YUJLIIRMIAGMCQ-CIUDSAMLSA-N Ser-Leu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O YUJLIIRMIAGMCQ-CIUDSAMLSA-N 0.000 description 6
- ILZAUMFXKSIUEF-SRVKXCTJSA-N Ser-Ser-Phe Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 ILZAUMFXKSIUEF-SRVKXCTJSA-N 0.000 description 6
- SNXUIBACCONSOH-BWBBJGPYSA-N Ser-Thr-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CO)C(O)=O SNXUIBACCONSOH-BWBBJGPYSA-N 0.000 description 6
- 108010092854 aspartyllysine Proteins 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 6
- VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 108010012581 phenylalanylglutamate Proteins 0.000 description 6
- 108010026333 seryl-proline Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- DMLSCRJBWUEALP-LAEOZQHASA-N Asn-Glu-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O DMLSCRJBWUEALP-LAEOZQHASA-N 0.000 description 4
- SNYCNNPOFYBCEK-ZLUOBGJFSA-N Asn-Ser-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O SNYCNNPOFYBCEK-ZLUOBGJFSA-N 0.000 description 4
- JBDLMLZNDRLDIX-HJGDQZAQSA-N Asn-Thr-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O JBDLMLZNDRLDIX-HJGDQZAQSA-N 0.000 description 4
- VFUXXFVCYZPOQG-WDSKDSINSA-N Asp-Glu-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O VFUXXFVCYZPOQG-WDSKDSINSA-N 0.000 description 4
- XFKUFUJECJUQTQ-CIUDSAMLSA-N Gln-Gln-Glu Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O XFKUFUJECJUQTQ-CIUDSAMLSA-N 0.000 description 4
- AJDMYLOISOCHHC-YVNDNENWSA-N Gln-Gln-Ile Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O AJDMYLOISOCHHC-YVNDNENWSA-N 0.000 description 4
- TVRMJKNELJKNRS-GUBZILKMSA-N His-Glu-Asn Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N TVRMJKNELJKNRS-GUBZILKMSA-N 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- KAFOIVJDVSZUMD-UHFFFAOYSA-N Leu-Gln-Gln Natural products CC(C)CC(N)C(=O)NC(CCC(N)=O)C(=O)NC(CCC(N)=O)C(O)=O KAFOIVJDVSZUMD-UHFFFAOYSA-N 0.000 description 4
- SBANPBVRHYIMRR-UHFFFAOYSA-N Leu-Ser-Pro Natural products CC(C)CC(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O SBANPBVRHYIMRR-UHFFFAOYSA-N 0.000 description 4
- PNPYKQFJGRFYJE-GUBZILKMSA-N Lys-Ala-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O PNPYKQFJGRFYJE-GUBZILKMSA-N 0.000 description 4
- LOGFVTREOLYCPF-RHYQMDGZSA-N Lys-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCCCN LOGFVTREOLYCPF-RHYQMDGZSA-N 0.000 description 4
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 4
- ZCXQTRXYZOSGJR-FXQIFTODSA-N Pro-Asp-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O ZCXQTRXYZOSGJR-FXQIFTODSA-N 0.000 description 4
- WLJYLAQSUSIQNH-GUBZILKMSA-N Pro-Met-Ser Chemical compound CSCC[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@@H]1CCCN1 WLJYLAQSUSIQNH-GUBZILKMSA-N 0.000 description 4
- ZIFYDQAFEMIZII-GUBZILKMSA-N Ser-Leu-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O ZIFYDQAFEMIZII-GUBZILKMSA-N 0.000 description 4
- CUXJENOFJXOSOZ-BIIVOSGPSA-N Ser-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CO)N)C(=O)O CUXJENOFJXOSOZ-BIIVOSGPSA-N 0.000 description 4
- LGIMRDKGABDMBN-DCAQKATOSA-N Ser-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CO)N LGIMRDKGABDMBN-DCAQKATOSA-N 0.000 description 4
- YGCDFAJJCRVQKU-RCWTZXSCSA-N Thr-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)[C@@H](C)O YGCDFAJJCRVQKU-RCWTZXSCSA-N 0.000 description 4
- 108010087924 alanylproline Proteins 0.000 description 4
- 108010040443 aspartyl-aspartic acid Proteins 0.000 description 4
- 229930002868 chlorophyll a Natural products 0.000 description 4
- 229930002869 chlorophyll b Natural products 0.000 description 4
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 230000003111 delayed effect Effects 0.000 description 4
- 108010080575 glutamyl-aspartyl-alanine Proteins 0.000 description 4
- 108010049041 glutamylalanine Proteins 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 108010073472 leucyl-prolyl-proline Proteins 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 108010031719 prolyl-serine Proteins 0.000 description 4
- 108010053725 prolylvaline Proteins 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 108010061238 threonyl-glycine Proteins 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 108010073969 valyllysine Proteins 0.000 description 4
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 3
- 108010060511 4-Aminobutyrate Transaminase Proteins 0.000 description 3
- 102100035923 4-aminobutyrate aminotransferase, mitochondrial Human genes 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 3
- 229930192334 Auxin Natural products 0.000 description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 3
- 240000008467 Oryza sativa Japonica Group Species 0.000 description 3
- 101100148881 Oryza sativa subsp. japonica Os01g0208600 gene Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 239000002363 auxin Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 150000001747 carotenoids Chemical class 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000005204 segregation Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- JNTMAZFVYNDPLB-PEDHHIEDSA-N (2S,3S)-2-[[[(2S)-1-[(2S,3S)-2-amino-3-methyl-1-oxopentyl]-2-pyrrolidinyl]-oxomethyl]amino]-3-methylpentanoic acid Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(O)=O JNTMAZFVYNDPLB-PEDHHIEDSA-N 0.000 description 2
- BRPMXFSTKXXNHF-IUCAKERBSA-N (2s)-1-[2-[[(2s)-pyrrolidine-2-carbonyl]amino]acetyl]pyrrolidine-2-carboxylic acid Chemical compound OC(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@H]1NCCC1 BRPMXFSTKXXNHF-IUCAKERBSA-N 0.000 description 2
- QWTLUPDHBKBULE-UHFFFAOYSA-N 2-[[2-[[2-[[2-[[2-[[2-[[2-[[2-[[2-[(2-aminoacetyl)amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetyl]amino]acetic acid Chemical compound NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(O)=O QWTLUPDHBKBULE-UHFFFAOYSA-N 0.000 description 2
- TTXMOJWKNRJWQJ-FXQIFTODSA-N Ala-Arg-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)C)CCCN=C(N)N TTXMOJWKNRJWQJ-FXQIFTODSA-N 0.000 description 2
- YAXNATKKPOWVCP-ZLUOBGJFSA-N Ala-Asn-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(O)=O YAXNATKKPOWVCP-ZLUOBGJFSA-N 0.000 description 2
- CVGNCMIULZNYES-WHFBIAKZSA-N Ala-Asn-Gly Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O CVGNCMIULZNYES-WHFBIAKZSA-N 0.000 description 2
- NHCPCLJZRSIDHS-ZLUOBGJFSA-N Ala-Asp-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(O)=O NHCPCLJZRSIDHS-ZLUOBGJFSA-N 0.000 description 2
- DECCMEWNXSNSDO-ZLUOBGJFSA-N Ala-Cys-Ala Chemical compound C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(O)=O DECCMEWNXSNSDO-ZLUOBGJFSA-N 0.000 description 2
- CXQODNIBUNQWAS-CIUDSAMLSA-N Ala-Gln-Arg Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N CXQODNIBUNQWAS-CIUDSAMLSA-N 0.000 description 2
- YIGLXQRFQVWFEY-NRPADANISA-N Ala-Gln-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O YIGLXQRFQVWFEY-NRPADANISA-N 0.000 description 2
- KXEVYGKATAMXJJ-ACZMJKKPSA-N Ala-Glu-Asp Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O KXEVYGKATAMXJJ-ACZMJKKPSA-N 0.000 description 2
- UHMQKOBNPRAZGB-CIUDSAMLSA-N Ala-Glu-Met Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCSC)C(=O)O)N UHMQKOBNPRAZGB-CIUDSAMLSA-N 0.000 description 2
- VBRDBGCROKWTPV-XHNCKOQMSA-N Ala-Glu-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N1CCC[C@@H]1C(=O)O)N VBRDBGCROKWTPV-XHNCKOQMSA-N 0.000 description 2
- VWEWCZSUWOEEFM-WDSKDSINSA-N Ala-Gly-Ala-Gly Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(O)=O VWEWCZSUWOEEFM-WDSKDSINSA-N 0.000 description 2
- FDAZDMAFZYTHGS-XVYDVKMFSA-N Ala-His-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(O)=O FDAZDMAFZYTHGS-XVYDVKMFSA-N 0.000 description 2
- DPNZTBKGAUAZQU-DLOVCJGASA-N Ala-Leu-His Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N DPNZTBKGAUAZQU-DLOVCJGASA-N 0.000 description 2
- OYJCVIGKMXUVKB-GARJFASQSA-N Ala-Leu-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N OYJCVIGKMXUVKB-GARJFASQSA-N 0.000 description 2
- MEFILNJXAVSUTO-JXUBOQSCSA-N Ala-Leu-Thr Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MEFILNJXAVSUTO-JXUBOQSCSA-N 0.000 description 2
- MFMDKJIPHSWSBM-GUBZILKMSA-N Ala-Lys-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O MFMDKJIPHSWSBM-GUBZILKMSA-N 0.000 description 2
- VCSABYLVNWQYQE-SRVKXCTJSA-N Ala-Lys-Lys Chemical compound NCCCC[C@H](NC(=O)[C@@H](N)C)C(=O)N[C@@H](CCCCN)C(O)=O VCSABYLVNWQYQE-SRVKXCTJSA-N 0.000 description 2
- VCSABYLVNWQYQE-UHFFFAOYSA-N Ala-Lys-Lys Natural products NCCCCC(NC(=O)C(N)C)C(=O)NC(CCCCN)C(O)=O VCSABYLVNWQYQE-UHFFFAOYSA-N 0.000 description 2
- CNQAFFMNJIQYGX-DRZSPHRISA-N Ala-Phe-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 CNQAFFMNJIQYGX-DRZSPHRISA-N 0.000 description 2
- IORKCNUBHNIMKY-CIUDSAMLSA-N Ala-Pro-Glu Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O IORKCNUBHNIMKY-CIUDSAMLSA-N 0.000 description 2
- WQLDNOCHHRISMS-NAKRPEOUSA-N Ala-Pro-Ile Chemical compound [H]N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(O)=O WQLDNOCHHRISMS-NAKRPEOUSA-N 0.000 description 2
- OLVCTPPSXNRGKV-GUBZILKMSA-N Ala-Pro-Pro Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 OLVCTPPSXNRGKV-GUBZILKMSA-N 0.000 description 2
- ARHJJAAWNWOACN-FXQIFTODSA-N Ala-Ser-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O ARHJJAAWNWOACN-FXQIFTODSA-N 0.000 description 2
- XQNRANMFRPCFFW-GCJQMDKQSA-N Ala-Thr-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](C)N)O XQNRANMFRPCFFW-GCJQMDKQSA-N 0.000 description 2
- FSXDWQGEWZQBPJ-HERUPUMHSA-N Ala-Trp-Asp Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CC(=O)O)C(=O)O)N FSXDWQGEWZQBPJ-HERUPUMHSA-N 0.000 description 2
- VHAQSYHSDKERBS-XPUUQOCRSA-N Ala-Val-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O VHAQSYHSDKERBS-XPUUQOCRSA-N 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- KGSJCPBERYUXCN-BPNCWPANSA-N Arg-Ala-Tyr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O KGSJCPBERYUXCN-BPNCWPANSA-N 0.000 description 2
- NABSCJGZKWSNHX-RCWTZXSCSA-N Arg-Arg-Thr Chemical compound NC(N)=NCCC[C@@H](C(=O)N[C@@H]([C@H](O)C)C(O)=O)NC(=O)[C@@H](N)CCCN=C(N)N NABSCJGZKWSNHX-RCWTZXSCSA-N 0.000 description 2
- SQKPKIJVWHAWNF-DCAQKATOSA-N Arg-Asp-Lys Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(O)=O SQKPKIJVWHAWNF-DCAQKATOSA-N 0.000 description 2
- MFAMTAVAFBPXDC-LPEHRKFASA-N Arg-Asp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)C(=O)O MFAMTAVAFBPXDC-LPEHRKFASA-N 0.000 description 2
- GIVWETPOBCRTND-DCAQKATOSA-N Arg-Gln-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O GIVWETPOBCRTND-DCAQKATOSA-N 0.000 description 2
- LMPKCSXZJSXBBL-NHCYSSNCSA-N Arg-Gln-Val Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O LMPKCSXZJSXBBL-NHCYSSNCSA-N 0.000 description 2
- DJAIOAKQIOGULM-DCAQKATOSA-N Arg-Glu-Met Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(O)=O DJAIOAKQIOGULM-DCAQKATOSA-N 0.000 description 2
- HPSVTWMFWCHKFN-GARJFASQSA-N Arg-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)C(=O)O HPSVTWMFWCHKFN-GARJFASQSA-N 0.000 description 2
- HQIZDMIGUJOSNI-IUCAKERBSA-N Arg-Gly-Arg Chemical compound N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O HQIZDMIGUJOSNI-IUCAKERBSA-N 0.000 description 2
- WVNFNPGXYADPPO-BQBZGAKWSA-N Arg-Gly-Ser Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O WVNFNPGXYADPPO-BQBZGAKWSA-N 0.000 description 2
- COXMUHNBYCVVRG-DCAQKATOSA-N Arg-Leu-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O COXMUHNBYCVVRG-DCAQKATOSA-N 0.000 description 2
- PZBSKYJGKNNYNK-ULQDDVLXSA-N Arg-Leu-Tyr Chemical compound CC(C)C[C@H](NC(=O)[C@@H](N)CCCN=C(N)N)C(=O)N[C@@H](Cc1ccc(O)cc1)C(O)=O PZBSKYJGKNNYNK-ULQDDVLXSA-N 0.000 description 2
- NGTYEHIRESTSRX-UWVGGRQHSA-N Arg-Lys-Gly Chemical compound NCCCC[C@@H](C(=O)NCC(O)=O)NC(=O)[C@@H](N)CCCN=C(N)N NGTYEHIRESTSRX-UWVGGRQHSA-N 0.000 description 2
- PAPSMOYMQDWIOR-AVGNSLFASA-N Arg-Lys-Val Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O PAPSMOYMQDWIOR-AVGNSLFASA-N 0.000 description 2
- FRBAHXABMQXSJQ-FXQIFTODSA-N Arg-Ser-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O FRBAHXABMQXSJQ-FXQIFTODSA-N 0.000 description 2
- JKRPBTQDPJSQIT-RCWTZXSCSA-N Arg-Thr-Met Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)O JKRPBTQDPJSQIT-RCWTZXSCSA-N 0.000 description 2
- ZJBUILVYSXQNSW-YTWAJWBKSA-N Arg-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)O ZJBUILVYSXQNSW-YTWAJWBKSA-N 0.000 description 2
- LLQIAIUAKGNOSE-NHCYSSNCSA-N Arg-Val-Gln Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CCCN=C(N)N LLQIAIUAKGNOSE-NHCYSSNCSA-N 0.000 description 2
- LEFKSBYHUGUWLP-ACZMJKKPSA-N Asn-Ala-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O LEFKSBYHUGUWLP-ACZMJKKPSA-N 0.000 description 2
- NUHQMYUWLUSRJX-BIIVOSGPSA-N Asn-Ala-Pro Chemical compound C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(=O)N)N NUHQMYUWLUSRJX-BIIVOSGPSA-N 0.000 description 2
- XWGJDUSDTRPQRK-ZLUOBGJFSA-N Asn-Ala-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(N)=O XWGJDUSDTRPQRK-ZLUOBGJFSA-N 0.000 description 2
- ACRYGQFHAQHDSF-ZLUOBGJFSA-N Asn-Asn-Asn Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O ACRYGQFHAQHDSF-ZLUOBGJFSA-N 0.000 description 2
- NLCDVZJDEXIDDL-BIIVOSGPSA-N Asn-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CC(=O)N)N)C(=O)O NLCDVZJDEXIDDL-BIIVOSGPSA-N 0.000 description 2
- UPALZCBCKAMGIY-PEFMBERDSA-N Asn-Gln-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O UPALZCBCKAMGIY-PEFMBERDSA-N 0.000 description 2
- GFFRWIJAFFMQGM-NUMRIWBASA-N Asn-Glu-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O GFFRWIJAFFMQGM-NUMRIWBASA-N 0.000 description 2
- OPEPUCYIGFEGSW-WDSKDSINSA-N Asn-Gly-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O OPEPUCYIGFEGSW-WDSKDSINSA-N 0.000 description 2
- RAQMSGVCGSJKCL-FOHZUACHSA-N Asn-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CC(N)=O RAQMSGVCGSJKCL-FOHZUACHSA-N 0.000 description 2
- IKLAUGBIDCDFOY-SRVKXCTJSA-N Asn-His-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(O)=O IKLAUGBIDCDFOY-SRVKXCTJSA-N 0.000 description 2
- XVBDDUPJVQXDSI-PEFMBERDSA-N Asn-Ile-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CC(=O)N)N XVBDDUPJVQXDSI-PEFMBERDSA-N 0.000 description 2
- SEKBHZJLARBNPB-GHCJXIJMSA-N Asn-Ile-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O SEKBHZJLARBNPB-GHCJXIJMSA-N 0.000 description 2
- BXUHCIXDSWRSBS-CIUDSAMLSA-N Asn-Leu-Asp Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O BXUHCIXDSWRSBS-CIUDSAMLSA-N 0.000 description 2
- DMRUJUFCPVHHKP-UHFFFAOYSA-N Asn-Met-Asn-Gln Chemical compound NC(=O)CC(N)C(=O)NC(CCSC)C(=O)NC(CC(N)=O)C(=O)NC(CCC(N)=O)C(O)=O DMRUJUFCPVHHKP-UHFFFAOYSA-N 0.000 description 2
- LSJQOMAZIKQMTJ-SRVKXCTJSA-N Asn-Phe-Asp Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(O)=O)C(O)=O LSJQOMAZIKQMTJ-SRVKXCTJSA-N 0.000 description 2
- YRTOMUMWSTUQAX-FXQIFTODSA-N Asn-Pro-Asp Chemical compound NC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O YRTOMUMWSTUQAX-FXQIFTODSA-N 0.000 description 2
- GFGUPLIETCNQGF-DCAQKATOSA-N Asn-Pro-His Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CC(=O)N)N)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O GFGUPLIETCNQGF-DCAQKATOSA-N 0.000 description 2
- VHQSGALUSWIYOD-QXEWZRGKSA-N Asn-Pro-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(O)=O VHQSGALUSWIYOD-QXEWZRGKSA-N 0.000 description 2
- JWQWPRCDYWNVNM-ACZMJKKPSA-N Asn-Ser-Gln Chemical compound C(CC(=O)N)[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)N)N JWQWPRCDYWNVNM-ACZMJKKPSA-N 0.000 description 2
- GZXOUBTUAUAVHD-ACZMJKKPSA-N Asn-Ser-Glu Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O GZXOUBTUAUAVHD-ACZMJKKPSA-N 0.000 description 2
- ZUFPUBYQYWCMDB-NUMRIWBASA-N Asn-Thr-Glu Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O ZUFPUBYQYWCMDB-NUMRIWBASA-N 0.000 description 2
- DPSUVAPLRQDWAO-YDHLFZDLSA-N Asn-Tyr-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)[C@H](CC(=O)N)N DPSUVAPLRQDWAO-YDHLFZDLSA-N 0.000 description 2
- PQKSVQSMTHPRIB-ZKWXMUAHSA-N Asn-Val-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O PQKSVQSMTHPRIB-ZKWXMUAHSA-N 0.000 description 2
- KDFQZBWWPYQBEN-ZLUOBGJFSA-N Asp-Ala-Asn Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)O)N KDFQZBWWPYQBEN-ZLUOBGJFSA-N 0.000 description 2
- PBVLJOIPOGUQQP-CIUDSAMLSA-N Asp-Ala-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(O)=O PBVLJOIPOGUQQP-CIUDSAMLSA-N 0.000 description 2
- XPGVTUBABLRGHY-BIIVOSGPSA-N Asp-Ala-Pro Chemical compound C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(=O)O)N XPGVTUBABLRGHY-BIIVOSGPSA-N 0.000 description 2
- BLQBMRNMBAYREH-UWJYBYFXSA-N Asp-Ala-Tyr Chemical compound N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O BLQBMRNMBAYREH-UWJYBYFXSA-N 0.000 description 2
- GWTLRDMPMJCNMH-WHFBIAKZSA-N Asp-Asn-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O GWTLRDMPMJCNMH-WHFBIAKZSA-N 0.000 description 2
- BUVNWKQBMZLCDW-UGYAYLCHSA-N Asp-Asn-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O BUVNWKQBMZLCDW-UGYAYLCHSA-N 0.000 description 2
- VHWNKSJHQFZJTH-FXQIFTODSA-N Asp-Asp-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)O)N VHWNKSJHQFZJTH-FXQIFTODSA-N 0.000 description 2
- BFOYULZBKYOKAN-OLHMAJIHSA-N Asp-Asp-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O BFOYULZBKYOKAN-OLHMAJIHSA-N 0.000 description 2
- PXLNPFOJZQMXAT-BYULHYEWSA-N Asp-Asp-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O PXLNPFOJZQMXAT-BYULHYEWSA-N 0.000 description 2
- NURJSGZGBVJFAD-ZLUOBGJFSA-N Asp-Cys-Ser Chemical compound C([C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)O)N)C(=O)O NURJSGZGBVJFAD-ZLUOBGJFSA-N 0.000 description 2
- LJRPYAZQQWHEEV-FXQIFTODSA-N Asp-Gln-Gln Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O LJRPYAZQQWHEEV-FXQIFTODSA-N 0.000 description 2
- HSWYMWGDMPLTTH-FXQIFTODSA-N Asp-Glu-Gln Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O HSWYMWGDMPLTTH-FXQIFTODSA-N 0.000 description 2
- WSGVTKZFVJSJOG-RCOVLWMOSA-N Asp-Gly-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O WSGVTKZFVJSJOG-RCOVLWMOSA-N 0.000 description 2
- KQBVNNAPIURMPD-PEFMBERDSA-N Asp-Ile-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(O)=O KQBVNNAPIURMPD-PEFMBERDSA-N 0.000 description 2
- PAYPSKIBMDHZPI-CIUDSAMLSA-N Asp-Leu-Asp Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O PAYPSKIBMDHZPI-CIUDSAMLSA-N 0.000 description 2
- AYFVRYXNDHBECD-YUMQZZPRSA-N Asp-Leu-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O AYFVRYXNDHBECD-YUMQZZPRSA-N 0.000 description 2
- CJUKAWUWBZCTDQ-SRVKXCTJSA-N Asp-Leu-Lys Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O CJUKAWUWBZCTDQ-SRVKXCTJSA-N 0.000 description 2
- UMHUHHJMEXNSIV-CIUDSAMLSA-N Asp-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(O)=O UMHUHHJMEXNSIV-CIUDSAMLSA-N 0.000 description 2
- DONWIPDSZZJHHK-HJGDQZAQSA-N Asp-Lys-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)O)N)O DONWIPDSZZJHHK-HJGDQZAQSA-N 0.000 description 2
- XFQOQUWGVCVYON-DCAQKATOSA-N Asp-Met-His Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 XFQOQUWGVCVYON-DCAQKATOSA-N 0.000 description 2
- HXVILZUZXFLVEN-DCAQKATOSA-N Asp-Met-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(O)=O HXVILZUZXFLVEN-DCAQKATOSA-N 0.000 description 2
- LKVKODXGSAFOFY-VEVYYDQMSA-N Asp-Met-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LKVKODXGSAFOFY-VEVYYDQMSA-N 0.000 description 2
- PWAIZUBWHRHYKS-MELADBBJSA-N Asp-Phe-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=CC=C2)NC(=O)[C@H](CC(=O)O)N)C(=O)O PWAIZUBWHRHYKS-MELADBBJSA-N 0.000 description 2
- NBKLEMWHDLAUEM-CIUDSAMLSA-N Asp-Ser-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)N NBKLEMWHDLAUEM-CIUDSAMLSA-N 0.000 description 2
- VNXQRBXEQXLERQ-CIUDSAMLSA-N Asp-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)N VNXQRBXEQXLERQ-CIUDSAMLSA-N 0.000 description 2
- HRVQDZOWMLFAOD-BIIVOSGPSA-N Asp-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)N)C(=O)O HRVQDZOWMLFAOD-BIIVOSGPSA-N 0.000 description 2
- OZBXOELNJBSJOA-UBHSHLNASA-N Asp-Ser-Trp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)N OZBXOELNJBSJOA-UBHSHLNASA-N 0.000 description 2
- XYPJXLLXNSAWHZ-SRVKXCTJSA-N Asp-Ser-Tyr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O XYPJXLLXNSAWHZ-SRVKXCTJSA-N 0.000 description 2
- UTLCRGFJFSZWAW-OLHMAJIHSA-N Asp-Thr-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O UTLCRGFJFSZWAW-OLHMAJIHSA-N 0.000 description 2
- NAAAPCLFJPURAM-HJGDQZAQSA-N Asp-Thr-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O NAAAPCLFJPURAM-HJGDQZAQSA-N 0.000 description 2
- RSMZEHCMIOKNMW-GSSVUCPTSA-N Asp-Thr-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O RSMZEHCMIOKNMW-GSSVUCPTSA-N 0.000 description 2
- ITGFVUYOLWBPQW-KKHAAJSZSA-N Asp-Thr-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O ITGFVUYOLWBPQW-KKHAAJSZSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000526900 Camellia oleifera Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- MBRWOKXNHTUJMB-CIUDSAMLSA-N Cys-Pro-Glu Chemical compound [H]N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O MBRWOKXNHTUJMB-CIUDSAMLSA-N 0.000 description 2
- NITLUESFANGEIW-BQBZGAKWSA-N Cys-Pro-Gly Chemical compound [H]N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O NITLUESFANGEIW-BQBZGAKWSA-N 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 2
- KWUSGAIFNHQCBY-DCAQKATOSA-N Gln-Arg-Arg Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O KWUSGAIFNHQCBY-DCAQKATOSA-N 0.000 description 2
- LZRMPXRYLLTAJX-GUBZILKMSA-N Gln-Arg-Glu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O LZRMPXRYLLTAJX-GUBZILKMSA-N 0.000 description 2
- AAOBFSKXAVIORT-GUBZILKMSA-N Gln-Asn-Leu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O AAOBFSKXAVIORT-GUBZILKMSA-N 0.000 description 2
- RMOCFPBLHAOTDU-ACZMJKKPSA-N Gln-Asn-Ser Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O RMOCFPBLHAOTDU-ACZMJKKPSA-N 0.000 description 2
- GMGKDVVBSVVKCT-NUMRIWBASA-N Gln-Asn-Thr Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O GMGKDVVBSVVKCT-NUMRIWBASA-N 0.000 description 2
- CGVWDTRDPLOMHZ-FXQIFTODSA-N Gln-Glu-Asp Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O CGVWDTRDPLOMHZ-FXQIFTODSA-N 0.000 description 2
- SNLOOPZHAQDMJG-CIUDSAMLSA-N Gln-Glu-Glu Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O SNLOOPZHAQDMJG-CIUDSAMLSA-N 0.000 description 2
- HHQCBFGKQDMWSP-GUBZILKMSA-N Gln-Leu-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCC(=O)N)N HHQCBFGKQDMWSP-GUBZILKMSA-N 0.000 description 2
- IULKWYSYZSURJK-AVGNSLFASA-N Gln-Leu-Lys Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O IULKWYSYZSURJK-AVGNSLFASA-N 0.000 description 2
- ZBKUIQNCRIYVGH-SDDRHHMPSA-N Gln-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)N)N ZBKUIQNCRIYVGH-SDDRHHMPSA-N 0.000 description 2
- IHSGESFHTMFHRB-GUBZILKMSA-N Gln-Lys-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(N)=O IHSGESFHTMFHRB-GUBZILKMSA-N 0.000 description 2
- LPIKVBWNNVFHCQ-GUBZILKMSA-N Gln-Ser-Leu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LPIKVBWNNVFHCQ-GUBZILKMSA-N 0.000 description 2
- JILRMFFFCHUUTJ-ACZMJKKPSA-N Gln-Ser-Ser Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O JILRMFFFCHUUTJ-ACZMJKKPSA-N 0.000 description 2
- OUBUHIODTNUUTC-WDCWCFNPSA-N Gln-Thr-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)N)N)O OUBUHIODTNUUTC-WDCWCFNPSA-N 0.000 description 2
- ATRHMOJQJWPVBQ-DRZSPHRISA-N Glu-Ala-Phe Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O ATRHMOJQJWPVBQ-DRZSPHRISA-N 0.000 description 2
- CGYDXNKRIMJMLV-GUBZILKMSA-N Glu-Arg-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O CGYDXNKRIMJMLV-GUBZILKMSA-N 0.000 description 2
- FLLRAEJOLZPSMN-CIUDSAMLSA-N Glu-Asn-Arg Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N FLLRAEJOLZPSMN-CIUDSAMLSA-N 0.000 description 2
- YKLNMGJYMNPBCP-ACZMJKKPSA-N Glu-Asn-Asp Chemical compound C(CC(=O)O)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(=O)O)C(=O)O)N YKLNMGJYMNPBCP-ACZMJKKPSA-N 0.000 description 2
- CKRUHITYRFNUKW-WDSKDSINSA-N Glu-Asn-Gly Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O CKRUHITYRFNUKW-WDSKDSINSA-N 0.000 description 2
- ZOXBSICWUDAOHX-GUBZILKMSA-N Glu-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CCC(O)=O ZOXBSICWUDAOHX-GUBZILKMSA-N 0.000 description 2
- JPHYJQHPILOKHC-ACZMJKKPSA-N Glu-Asp-Asp Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O JPHYJQHPILOKHC-ACZMJKKPSA-N 0.000 description 2
- JRCUFCXYZLPSDZ-ACZMJKKPSA-N Glu-Asp-Ser Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O JRCUFCXYZLPSDZ-ACZMJKKPSA-N 0.000 description 2
- SBCYJMOOHUDWDA-NUMRIWBASA-N Glu-Asp-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O SBCYJMOOHUDWDA-NUMRIWBASA-N 0.000 description 2
- GYCPQVFKCPPRQB-GUBZILKMSA-N Glu-Gln-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCC(=O)O)N GYCPQVFKCPPRQB-GUBZILKMSA-N 0.000 description 2
- NUSWUSKZRCGFEX-FXQIFTODSA-N Glu-Glu-Cys Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CS)C(O)=O NUSWUSKZRCGFEX-FXQIFTODSA-N 0.000 description 2
- SJPMNHCEWPTRBR-BQBZGAKWSA-N Glu-Glu-Gly Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O SJPMNHCEWPTRBR-BQBZGAKWSA-N 0.000 description 2
- HPJLZFTUUJKWAJ-JHEQGTHGSA-N Glu-Gly-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(O)=O HPJLZFTUUJKWAJ-JHEQGTHGSA-N 0.000 description 2
- GXMXPCXXKVWOSM-KQXIARHKSA-N Glu-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)O)N GXMXPCXXKVWOSM-KQXIARHKSA-N 0.000 description 2
- ZHNHJYYFCGUZNQ-KBIXCLLPSA-N Glu-Ile-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H](N)CCC(O)=O ZHNHJYYFCGUZNQ-KBIXCLLPSA-N 0.000 description 2
- VGBSZQSKQRMLHD-MNXVOIDGSA-N Glu-Leu-Ile Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VGBSZQSKQRMLHD-MNXVOIDGSA-N 0.000 description 2
- MWMJCGBSIORNCD-AVGNSLFASA-N Glu-Leu-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O MWMJCGBSIORNCD-AVGNSLFASA-N 0.000 description 2
- UGSVSNXPJJDJKL-SDDRHHMPSA-N Glu-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)O)N UGSVSNXPJJDJKL-SDDRHHMPSA-N 0.000 description 2
- HRBYTAIBKPNZKQ-AVGNSLFASA-N Glu-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(O)=O HRBYTAIBKPNZKQ-AVGNSLFASA-N 0.000 description 2
- CBWKURKPYSLMJV-SOUVJXGZSA-N Glu-Phe-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=CC=C2)NC(=O)[C@H](CCC(=O)O)N)C(=O)O CBWKURKPYSLMJV-SOUVJXGZSA-N 0.000 description 2
- BIYNPVYAZOUVFQ-CIUDSAMLSA-N Glu-Pro-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O BIYNPVYAZOUVFQ-CIUDSAMLSA-N 0.000 description 2
- BPLNJYHNAJVLRT-ACZMJKKPSA-N Glu-Ser-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O BPLNJYHNAJVLRT-ACZMJKKPSA-N 0.000 description 2
- GMVCSRBOSIUTFC-FXQIFTODSA-N Glu-Ser-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(O)=O GMVCSRBOSIUTFC-FXQIFTODSA-N 0.000 description 2
- VNCNWQPIQYAMAK-ACZMJKKPSA-N Glu-Ser-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O VNCNWQPIQYAMAK-ACZMJKKPSA-N 0.000 description 2
- JWNZHMSRZXXGTM-XKBZYTNZSA-N Glu-Ser-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JWNZHMSRZXXGTM-XKBZYTNZSA-N 0.000 description 2
- JVYNYWXHZWVJEF-NUMRIWBASA-N Glu-Thr-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O JVYNYWXHZWVJEF-NUMRIWBASA-N 0.000 description 2
- MLILEEIVMRUYBX-NHCYSSNCSA-N Glu-Val-Arg Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O MLILEEIVMRUYBX-NHCYSSNCSA-N 0.000 description 2
- RMWAOBGCZZSJHE-UMNHJUIQSA-N Glu-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)O)N RMWAOBGCZZSJHE-UMNHJUIQSA-N 0.000 description 2
- QXUPRMQJDWJDFR-NRPADANISA-N Glu-Val-Ser Chemical compound CC(C)[C@H](NC(=O)[C@@H](N)CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O QXUPRMQJDWJDFR-NRPADANISA-N 0.000 description 2
- PYTZFYUXZZHOAD-WHFBIAKZSA-N Gly-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CN PYTZFYUXZZHOAD-WHFBIAKZSA-N 0.000 description 2
- JBRBACJPBZNFMF-YUMQZZPRSA-N Gly-Ala-Lys Chemical compound NCC(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCCCN JBRBACJPBZNFMF-YUMQZZPRSA-N 0.000 description 2
- QXPRJQPCFXMCIY-NKWVEPMBSA-N Gly-Ala-Pro Chemical compound C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)CN QXPRJQPCFXMCIY-NKWVEPMBSA-N 0.000 description 2
- JVACNFOPSUPDTK-QWRGUYRKSA-N Gly-Asn-Phe Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 JVACNFOPSUPDTK-QWRGUYRKSA-N 0.000 description 2
- XCLCVBYNGXEVDU-WHFBIAKZSA-N Gly-Asn-Ser Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O XCLCVBYNGXEVDU-WHFBIAKZSA-N 0.000 description 2
- FUTAPPOITCCWTH-WHFBIAKZSA-N Gly-Asp-Asp Chemical compound [H]NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O FUTAPPOITCCWTH-WHFBIAKZSA-N 0.000 description 2
- GVVKYKCOFMMTKZ-WHFBIAKZSA-N Gly-Cys-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)CN GVVKYKCOFMMTKZ-WHFBIAKZSA-N 0.000 description 2
- XLFHCWHXKSFVIB-BQBZGAKWSA-N Gly-Gln-Gln Chemical compound NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O XLFHCWHXKSFVIB-BQBZGAKWSA-N 0.000 description 2
- GDOZQTNZPCUARW-YFKPBYRVSA-N Gly-Gly-Glu Chemical compound NCC(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O GDOZQTNZPCUARW-YFKPBYRVSA-N 0.000 description 2
- XMPXVJIDADUOQB-RCOVLWMOSA-N Gly-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C([O-])=O)NC(=O)CNC(=O)C[NH3+] XMPXVJIDADUOQB-RCOVLWMOSA-N 0.000 description 2
- OLPPXYMMIARYAL-QMMMGPOBSA-N Gly-Gly-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)CNC(=O)CN OLPPXYMMIARYAL-QMMMGPOBSA-N 0.000 description 2
- FQKKPCWTZZEDIC-XPUUQOCRSA-N Gly-His-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 FQKKPCWTZZEDIC-XPUUQOCRSA-N 0.000 description 2
- VAXIVIPMCTYSHI-YUMQZZPRSA-N Gly-His-Asp Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)CN VAXIVIPMCTYSHI-YUMQZZPRSA-N 0.000 description 2
- ORXZVPZCPMKHNR-IUCAKERBSA-N Gly-His-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CNC=N1 ORXZVPZCPMKHNR-IUCAKERBSA-N 0.000 description 2
- IUZGUFAJDBHQQV-YUMQZZPRSA-N Gly-Leu-Asn Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O IUZGUFAJDBHQQV-YUMQZZPRSA-N 0.000 description 2
- CCBIBMKQNXHNIN-ZETCQYMHSA-N Gly-Leu-Gly Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O CCBIBMKQNXHNIN-ZETCQYMHSA-N 0.000 description 2
- UUYBFNKHOCJCHT-VHSXEESVSA-N Gly-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)CN UUYBFNKHOCJCHT-VHSXEESVSA-N 0.000 description 2
- FHQRLHFYVZAQHU-IUCAKERBSA-N Gly-Lys-Gln Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(O)=O FHQRLHFYVZAQHU-IUCAKERBSA-N 0.000 description 2
- GAAHQHNCMIAYEX-UWVGGRQHSA-N Gly-Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN GAAHQHNCMIAYEX-UWVGGRQHSA-N 0.000 description 2
- SOEGEPHNZOISMT-BYPYZUCNSA-N Gly-Ser-Gly Chemical compound NCC(=O)N[C@@H](CO)C(=O)NCC(O)=O SOEGEPHNZOISMT-BYPYZUCNSA-N 0.000 description 2
- JSLVAHYTAJJEQH-QWRGUYRKSA-N Gly-Ser-Phe Chemical compound NCC(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 JSLVAHYTAJJEQH-QWRGUYRKSA-N 0.000 description 2
- NVTPVQLIZCOJFK-FOHZUACHSA-N Gly-Thr-Asp Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O NVTPVQLIZCOJFK-FOHZUACHSA-N 0.000 description 2
- GBYYQVBXFVDJPJ-WLTAIBSBSA-N Gly-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)CN)O GBYYQVBXFVDJPJ-WLTAIBSBSA-N 0.000 description 2
- SBVMXEZQJVUARN-XPUUQOCRSA-N Gly-Val-Ser Chemical compound NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O SBVMXEZQJVUARN-XPUUQOCRSA-N 0.000 description 2
- RVKIPWVMZANZLI-UHFFFAOYSA-N H-Lys-Trp-OH Natural products C1=CC=C2C(CC(NC(=O)C(N)CCCCN)C(O)=O)=CNC2=C1 RVKIPWVMZANZLI-UHFFFAOYSA-N 0.000 description 2
- VYMGAXSNYUFVCK-GUBZILKMSA-N His-Gln-Asn Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)O)N VYMGAXSNYUFVCK-GUBZILKMSA-N 0.000 description 2
- BQFGKVYHKCNEMF-DCAQKATOSA-N His-Glu-Gln Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 BQFGKVYHKCNEMF-DCAQKATOSA-N 0.000 description 2
- KAFZDWMZKGQDEE-SRVKXCTJSA-N His-His-Asp Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CC2=CN=CN2)C(=O)N[C@@H](CC(=O)O)C(=O)O)N KAFZDWMZKGQDEE-SRVKXCTJSA-N 0.000 description 2
- YAALVYQFVJNXIV-KKUMJFAQSA-N His-Leu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CN=CN1 YAALVYQFVJNXIV-KKUMJFAQSA-N 0.000 description 2
- SAPLASXFNUYUFE-CQDKDKBSSA-N His-Phe-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@H](CC2=CN=CN2)N SAPLASXFNUYUFE-CQDKDKBSSA-N 0.000 description 2
- STGQSBKUYSPPIG-CIUDSAMLSA-N His-Ser-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CN=CN1 STGQSBKUYSPPIG-CIUDSAMLSA-N 0.000 description 2
- VIJMRAIWYWRXSR-CIUDSAMLSA-N His-Ser-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CN=CN1 VIJMRAIWYWRXSR-CIUDSAMLSA-N 0.000 description 2
- ILUVWFTXAUYOBW-CUJWVEQBSA-N His-Ser-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC1=CN=CN1)N)O ILUVWFTXAUYOBW-CUJWVEQBSA-N 0.000 description 2
- WSXNWASHQNSMRX-GVXVVHGQSA-N His-Val-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC1=CN=CN1)N WSXNWASHQNSMRX-GVXVVHGQSA-N 0.000 description 2
- TZCGZYWNIDZZMR-NAKRPEOUSA-N Ile-Arg-Ala Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](C)C(=O)O)N TZCGZYWNIDZZMR-NAKRPEOUSA-N 0.000 description 2
- TZCGZYWNIDZZMR-UHFFFAOYSA-N Ile-Arg-Ala Natural products CCC(C)C(N)C(=O)NC(C(=O)NC(C)C(O)=O)CCCN=C(N)N TZCGZYWNIDZZMR-UHFFFAOYSA-N 0.000 description 2
- ASCFJMSGKUIRDU-ZPFDUUQYSA-N Ile-Arg-Gln Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(O)=O ASCFJMSGKUIRDU-ZPFDUUQYSA-N 0.000 description 2
- JQLFYZMEXFNRFS-DJFWLOJKSA-N Ile-Asp-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N JQLFYZMEXFNRFS-DJFWLOJKSA-N 0.000 description 2
- IGJWJGIHUFQANP-LAEOZQHASA-N Ile-Gly-Gln Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)N[C@@H](CCC(=O)N)C(=O)O)N IGJWJGIHUFQANP-LAEOZQHASA-N 0.000 description 2
- CCYGNFBYUNHFSC-MGHWNKPDSA-N Ile-His-Phe Chemical compound [H]N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O CCYGNFBYUNHFSC-MGHWNKPDSA-N 0.000 description 2
- AXNGDPAKKCEKGY-QPHKQPEJSA-N Ile-Ile-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N AXNGDPAKKCEKGY-QPHKQPEJSA-N 0.000 description 2
- BJECXJHLUJXPJQ-PYJNHQTQSA-N Ile-Pro-His Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N BJECXJHLUJXPJQ-PYJNHQTQSA-N 0.000 description 2
- JZNVOBUNTWNZPW-GHCJXIJMSA-N Ile-Ser-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)O)N JZNVOBUNTWNZPW-GHCJXIJMSA-N 0.000 description 2
- PXKACEXYLPBMAD-JBDRJPRFSA-N Ile-Ser-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O)N PXKACEXYLPBMAD-JBDRJPRFSA-N 0.000 description 2
- WLRJHVNFGAOYPS-HJPIBITLSA-N Ile-Ser-Tyr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O)N WLRJHVNFGAOYPS-HJPIBITLSA-N 0.000 description 2
- JJQQGCMKLOEGAV-OSUNSFLBSA-N Ile-Thr-Met Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)O)N JJQQGCMKLOEGAV-OSUNSFLBSA-N 0.000 description 2
- JZBVBOKASHNXAD-NAKRPEOUSA-N Ile-Val-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(=O)O)N JZBVBOKASHNXAD-NAKRPEOUSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- HGCNKOLVKRAVHD-UHFFFAOYSA-N L-Met-L-Phe Natural products CSCCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-UHFFFAOYSA-N 0.000 description 2
- SENJXOPIZNYLHU-UHFFFAOYSA-N L-leucyl-L-arginine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CCCN=C(N)N SENJXOPIZNYLHU-UHFFFAOYSA-N 0.000 description 2
- 241000880493 Leptailurus serval Species 0.000 description 2
- WSGXUIQTEZDVHJ-GARJFASQSA-N Leu-Ala-Pro Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@@H]1C(O)=O WSGXUIQTEZDVHJ-GARJFASQSA-N 0.000 description 2
- VCSBGUACOYUIGD-CIUDSAMLSA-N Leu-Asn-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O VCSBGUACOYUIGD-CIUDSAMLSA-N 0.000 description 2
- MYGQXVYRZMKRDB-SRVKXCTJSA-N Leu-Asp-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN MYGQXVYRZMKRDB-SRVKXCTJSA-N 0.000 description 2
- CLVUXCBGKUECIT-HJGDQZAQSA-N Leu-Asp-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O CLVUXCBGKUECIT-HJGDQZAQSA-N 0.000 description 2
- VPKIQULSKFVCSM-SRVKXCTJSA-N Leu-Gln-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O VPKIQULSKFVCSM-SRVKXCTJSA-N 0.000 description 2
- HFBCHNRFRYLZNV-GUBZILKMSA-N Leu-Glu-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O HFBCHNRFRYLZNV-GUBZILKMSA-N 0.000 description 2
- HQUXQAMSWFIRET-AVGNSLFASA-N Leu-Glu-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN HQUXQAMSWFIRET-AVGNSLFASA-N 0.000 description 2
- WQWSMEOYXJTFRU-GUBZILKMSA-N Leu-Glu-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O WQWSMEOYXJTFRU-GUBZILKMSA-N 0.000 description 2
- LLBQJYDYOLIQAI-JYJNAYRXSA-N Leu-Glu-Tyr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O LLBQJYDYOLIQAI-JYJNAYRXSA-N 0.000 description 2
- LAPSXOAUPNOINL-YUMQZZPRSA-N Leu-Gly-Asp Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(O)=O LAPSXOAUPNOINL-YUMQZZPRSA-N 0.000 description 2
- HYMLKESRWLZDBR-WEDXCCLWSA-N Leu-Gly-Thr Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(O)=O HYMLKESRWLZDBR-WEDXCCLWSA-N 0.000 description 2
- KVOFSTUWVSQMDK-KKUMJFAQSA-N Leu-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(C)C)CC1=CN=CN1 KVOFSTUWVSQMDK-KKUMJFAQSA-N 0.000 description 2
- PDQDCFBVYXEFSD-SRVKXCTJSA-N Leu-Leu-Asp Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O PDQDCFBVYXEFSD-SRVKXCTJSA-N 0.000 description 2
- INCJJHQRZGQLFC-KBPBESRZSA-N Leu-Phe-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(O)=O INCJJHQRZGQLFC-KBPBESRZSA-N 0.000 description 2
- WMIOEVKKYIMVKI-DCAQKATOSA-N Leu-Pro-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O WMIOEVKKYIMVKI-DCAQKATOSA-N 0.000 description 2
- QMKFDEUJGYNFMC-AVGNSLFASA-N Leu-Pro-Arg Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O QMKFDEUJGYNFMC-AVGNSLFASA-N 0.000 description 2
- RRVCZCNFXIFGRA-DCAQKATOSA-N Leu-Pro-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O RRVCZCNFXIFGRA-DCAQKATOSA-N 0.000 description 2
- UCBPDSYUVAAHCD-UWVGGRQHSA-N Leu-Pro-Gly Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O UCBPDSYUVAAHCD-UWVGGRQHSA-N 0.000 description 2
- YUTNOGOMBNYPFH-XUXIUFHCSA-N Leu-Pro-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(O)=O YUTNOGOMBNYPFH-XUXIUFHCSA-N 0.000 description 2
- KZZCOWMDDXDKSS-CIUDSAMLSA-N Leu-Ser-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O KZZCOWMDDXDKSS-CIUDSAMLSA-N 0.000 description 2
- SBANPBVRHYIMRR-GARJFASQSA-N Leu-Ser-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N SBANPBVRHYIMRR-GARJFASQSA-N 0.000 description 2
- BRTVHXHCUSXYRI-CIUDSAMLSA-N Leu-Ser-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O BRTVHXHCUSXYRI-CIUDSAMLSA-N 0.000 description 2
- ICYRCNICGBJLGM-HJGDQZAQSA-N Leu-Thr-Asp Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CC(O)=O ICYRCNICGBJLGM-HJGDQZAQSA-N 0.000 description 2
- LSLUTXRANSUGFY-XIRDDKMYSA-N Leu-Trp-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(O)=O)C(O)=O LSLUTXRANSUGFY-XIRDDKMYSA-N 0.000 description 2
- SUYRAPCRSCCPAK-VFAJRCTISA-N Leu-Trp-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H]([C@@H](C)O)C(O)=O SUYRAPCRSCCPAK-VFAJRCTISA-N 0.000 description 2
- VUBIPAHVHMZHCM-KKUMJFAQSA-N Leu-Tyr-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(O)=O)CC1=CC=C(O)C=C1 VUBIPAHVHMZHCM-KKUMJFAQSA-N 0.000 description 2
- FBNPMTNBFFAMMH-UHFFFAOYSA-N Leu-Val-Arg Natural products CC(C)CC(N)C(=O)NC(C(C)C)C(=O)NC(C(O)=O)CCCN=C(N)N FBNPMTNBFFAMMH-UHFFFAOYSA-N 0.000 description 2
- IRNSXVOWSXSULE-DCAQKATOSA-N Lys-Ala-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN IRNSXVOWSXSULE-DCAQKATOSA-N 0.000 description 2
- YKIRNDPUWONXQN-GUBZILKMSA-N Lys-Asn-Gln Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N YKIRNDPUWONXQN-GUBZILKMSA-N 0.000 description 2
- DEFGUIIUYAUEDU-ZPFDUUQYSA-N Lys-Asn-Ile Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O DEFGUIIUYAUEDU-ZPFDUUQYSA-N 0.000 description 2
- SFQPJNQDUUYCLA-BJDJZHNGSA-N Lys-Cys-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CCCCN)N SFQPJNQDUUYCLA-BJDJZHNGSA-N 0.000 description 2
- RZHLIPMZXOEJTL-AVGNSLFASA-N Lys-Gln-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCCCN)N RZHLIPMZXOEJTL-AVGNSLFASA-N 0.000 description 2
- LPAJOCKCPRZEAG-MNXVOIDGSA-N Lys-Glu-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCCCN LPAJOCKCPRZEAG-MNXVOIDGSA-N 0.000 description 2
- QOJDBRUCOXQSSK-AJNGGQMLSA-N Lys-Ile-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(O)=O QOJDBRUCOXQSSK-AJNGGQMLSA-N 0.000 description 2
- NJNRBRKHOWSGMN-SRVKXCTJSA-N Lys-Leu-Asn Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O NJNRBRKHOWSGMN-SRVKXCTJSA-N 0.000 description 2
- SKRGVGLIRUGANF-AVGNSLFASA-N Lys-Leu-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O SKRGVGLIRUGANF-AVGNSLFASA-N 0.000 description 2
- WRODMZBHNNPRLN-SRVKXCTJSA-N Lys-Leu-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O WRODMZBHNNPRLN-SRVKXCTJSA-N 0.000 description 2
- YDDDRTIPNTWGIG-SRVKXCTJSA-N Lys-Lys-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O YDDDRTIPNTWGIG-SRVKXCTJSA-N 0.000 description 2
- UQJOKDAYFULYIX-AVGNSLFASA-N Lys-Pro-Pro Chemical compound NCCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 UQJOKDAYFULYIX-AVGNSLFASA-N 0.000 description 2
- YSPZCHGIWAQVKQ-AVGNSLFASA-N Lys-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCCCN YSPZCHGIWAQVKQ-AVGNSLFASA-N 0.000 description 2
- LKDXINHHSWFFJC-SRVKXCTJSA-N Lys-Ser-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)N LKDXINHHSWFFJC-SRVKXCTJSA-N 0.000 description 2
- ZUGVARDEGWMMLK-SRVKXCTJSA-N Lys-Ser-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCCN ZUGVARDEGWMMLK-SRVKXCTJSA-N 0.000 description 2
- CUHGAUZONORRIC-HJGDQZAQSA-N Lys-Thr-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCCCN)N)O CUHGAUZONORRIC-HJGDQZAQSA-N 0.000 description 2
- DRRXXZBXDMLGFC-IHRRRGAJSA-N Lys-Val-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CCCCN DRRXXZBXDMLGFC-IHRRRGAJSA-N 0.000 description 2
- DLAFCQWUMFMZSN-GUBZILKMSA-N Met-Arg-Ala Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](C)C(O)=O)CCCN=C(N)N DLAFCQWUMFMZSN-GUBZILKMSA-N 0.000 description 2
- AWGBEIYZPAXXSX-RWMBFGLXSA-N Met-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCSC)N AWGBEIYZPAXXSX-RWMBFGLXSA-N 0.000 description 2
- HOZNVKDCKZPRER-XUXIUFHCSA-N Met-Lys-Ile Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HOZNVKDCKZPRER-XUXIUFHCSA-N 0.000 description 2
- YLDSJJOGQNEQJK-AVGNSLFASA-N Met-Pro-Leu Chemical compound CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(O)=O YLDSJJOGQNEQJK-AVGNSLFASA-N 0.000 description 2
- XIGAHPDZLAYQOS-SRVKXCTJSA-N Met-Pro-Pro Chemical compound CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 XIGAHPDZLAYQOS-SRVKXCTJSA-N 0.000 description 2
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 2
- AUEJLPRZGVVDNU-UHFFFAOYSA-N N-L-tyrosyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CC1=CC=C(O)C=C1 AUEJLPRZGVVDNU-UHFFFAOYSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- MECSIDWUTYRHRJ-KKUMJFAQSA-N Phe-Asn-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O MECSIDWUTYRHRJ-KKUMJFAQSA-N 0.000 description 2
- WMGVYPPIMZPWPN-SRVKXCTJSA-N Phe-Asp-Asn Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N WMGVYPPIMZPWPN-SRVKXCTJSA-N 0.000 description 2
- AKJAKCBHLJGRBU-JYJNAYRXSA-N Phe-Glu-His Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N AKJAKCBHLJGRBU-JYJNAYRXSA-N 0.000 description 2
- KXUZHWXENMYOHC-QEJZJMRPSA-N Phe-Leu-Ala Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O KXUZHWXENMYOHC-QEJZJMRPSA-N 0.000 description 2
- PEFJUUYFEGBXFA-BZSNNMDCSA-N Phe-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1=CC=CC=C1 PEFJUUYFEGBXFA-BZSNNMDCSA-N 0.000 description 2
- LYCOGHUNJCETDK-JYJNAYRXSA-N Phe-Met-Met Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)N LYCOGHUNJCETDK-JYJNAYRXSA-N 0.000 description 2
- XDMMOISUAHXXFD-SRVKXCTJSA-N Phe-Ser-Asp Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O XDMMOISUAHXXFD-SRVKXCTJSA-N 0.000 description 2
- IPFXYNKCXYGSSV-KKUMJFAQSA-N Phe-Ser-Lys Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O)N IPFXYNKCXYGSSV-KKUMJFAQSA-N 0.000 description 2
- MCIXMYKSPQUMJG-SRVKXCTJSA-N Phe-Ser-Ser Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O MCIXMYKSPQUMJG-SRVKXCTJSA-N 0.000 description 2
- KIZQGKLMXKGDIV-BQBZGAKWSA-N Pro-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1 KIZQGKLMXKGDIV-BQBZGAKWSA-N 0.000 description 2
- GDXZRWYXJSGWIV-GMOBBJLQSA-N Pro-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H]1CCCN1 GDXZRWYXJSGWIV-GMOBBJLQSA-N 0.000 description 2
- KPDRZQUWJKTMBP-DCAQKATOSA-N Pro-Asp-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H]1CCCN1 KPDRZQUWJKTMBP-DCAQKATOSA-N 0.000 description 2
- HJSCRFZVGXAGNG-SRVKXCTJSA-N Pro-Gln-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H]1CCCN1 HJSCRFZVGXAGNG-SRVKXCTJSA-N 0.000 description 2
- UAYHMOIGIQZLFR-NHCYSSNCSA-N Pro-Gln-Val Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O UAYHMOIGIQZLFR-NHCYSSNCSA-N 0.000 description 2
- NXEYSLRNNPWCRN-SRVKXCTJSA-N Pro-Glu-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O NXEYSLRNNPWCRN-SRVKXCTJSA-N 0.000 description 2
- VPEVBAUSTBWQHN-NHCYSSNCSA-N Pro-Glu-Val Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O VPEVBAUSTBWQHN-NHCYSSNCSA-N 0.000 description 2
- WSRWHZRUOCACLJ-UWVGGRQHSA-N Pro-Gly-His Chemical compound C([C@@H](C(=O)O)NC(=O)CNC(=O)[C@H]1NCCC1)C1=CN=CN1 WSRWHZRUOCACLJ-UWVGGRQHSA-N 0.000 description 2
- DXTOOBDIIAJZBJ-BQBZGAKWSA-N Pro-Gly-Ser Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CO)C(O)=O DXTOOBDIIAJZBJ-BQBZGAKWSA-N 0.000 description 2
- IBGCFJDLCYTKPW-NAKRPEOUSA-N Pro-Ile-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]1CCCN1 IBGCFJDLCYTKPW-NAKRPEOUSA-N 0.000 description 2
- KLSOMAFWRISSNI-OSUNSFLBSA-N Pro-Ile-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]1CCCN1 KLSOMAFWRISSNI-OSUNSFLBSA-N 0.000 description 2
- FYPGHGXAOZTOBO-IHRRRGAJSA-N Pro-Leu-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@@H]2CCCN2 FYPGHGXAOZTOBO-IHRRRGAJSA-N 0.000 description 2
- MCWHYUWXVNRXFV-RWMBFGLXSA-N Pro-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2 MCWHYUWXVNRXFV-RWMBFGLXSA-N 0.000 description 2
- KLOQCCRTPHPIFN-DCAQKATOSA-N Pro-Met-Gln Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@@H]1CCCN1 KLOQCCRTPHPIFN-DCAQKATOSA-N 0.000 description 2
- ANESFYPBAJPYNJ-SDDRHHMPSA-N Pro-Met-Pro Chemical compound CSCC[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2 ANESFYPBAJPYNJ-SDDRHHMPSA-N 0.000 description 2
- FHZJRBVMLGOHBX-GUBZILKMSA-N Pro-Pro-Asp Chemical compound OC(=O)C[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1)C(O)=O FHZJRBVMLGOHBX-GUBZILKMSA-N 0.000 description 2
- SBVPYBFMIGDIDX-SRVKXCTJSA-N Pro-Pro-Pro Chemical compound OC(=O)[C@@H]1CCCN1C(=O)[C@H]1N(C(=O)[C@H]2NCCC2)CCC1 SBVPYBFMIGDIDX-SRVKXCTJSA-N 0.000 description 2
- KBUAPZAZPWNYSW-SRVKXCTJSA-N Pro-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H]1NCCC1 KBUAPZAZPWNYSW-SRVKXCTJSA-N 0.000 description 2
- LNICFEXCAHIJOR-DCAQKATOSA-N Pro-Ser-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LNICFEXCAHIJOR-DCAQKATOSA-N 0.000 description 2
- UGDMQJSXSSZUKL-IHRRRGAJSA-N Pro-Ser-Tyr Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)O UGDMQJSXSSZUKL-IHRRRGAJSA-N 0.000 description 2
- ZUGXSSFMTXKHJS-ZLUOBGJFSA-N Ser-Ala-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(O)=O ZUGXSSFMTXKHJS-ZLUOBGJFSA-N 0.000 description 2
- LVVBAKCGXXUHFO-ZLUOBGJFSA-N Ser-Ala-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(O)=O LVVBAKCGXXUHFO-ZLUOBGJFSA-N 0.000 description 2
- WTWGOQRNRFHFQD-JBDRJPRFSA-N Ser-Ala-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O WTWGOQRNRFHFQD-JBDRJPRFSA-N 0.000 description 2
- JPIDMRXXNMIVKY-VZFHVOOUSA-N Ser-Ala-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JPIDMRXXNMIVKY-VZFHVOOUSA-N 0.000 description 2
- NLQUOHDCLSFABG-GUBZILKMSA-N Ser-Arg-Arg Chemical compound NC(N)=NCCC[C@H](NC(=O)[C@H](CO)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O NLQUOHDCLSFABG-GUBZILKMSA-N 0.000 description 2
- WDXYVIIVDIDOSX-DCAQKATOSA-N Ser-Arg-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CO)CCCN=C(N)N WDXYVIIVDIDOSX-DCAQKATOSA-N 0.000 description 2
- HBOABDXGTMMDSE-GUBZILKMSA-N Ser-Arg-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(O)=O HBOABDXGTMMDSE-GUBZILKMSA-N 0.000 description 2
- DKKGAAJTDKHWOD-BIIVOSGPSA-N Ser-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CO)N)C(=O)O DKKGAAJTDKHWOD-BIIVOSGPSA-N 0.000 description 2
- SFZKGGOGCNQPJY-CIUDSAMLSA-N Ser-Asp-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)N SFZKGGOGCNQPJY-CIUDSAMLSA-N 0.000 description 2
- QPFJSHSJFIYDJZ-GHCJXIJMSA-N Ser-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CO QPFJSHSJFIYDJZ-GHCJXIJMSA-N 0.000 description 2
- BGOWRLSWJCVYAQ-CIUDSAMLSA-N Ser-Asp-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O BGOWRLSWJCVYAQ-CIUDSAMLSA-N 0.000 description 2
- DGHFNYXVIXNNMC-GUBZILKMSA-N Ser-Gln-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CO)N DGHFNYXVIXNNMC-GUBZILKMSA-N 0.000 description 2
- GWMXFEMMBHOKDX-AVGNSLFASA-N Ser-Gln-Phe Chemical compound OC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 GWMXFEMMBHOKDX-AVGNSLFASA-N 0.000 description 2
- LALNXSXEYFUUDD-GUBZILKMSA-N Ser-Glu-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O LALNXSXEYFUUDD-GUBZILKMSA-N 0.000 description 2
- QKQDTEYDEIJPNK-GUBZILKMSA-N Ser-Glu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CO QKQDTEYDEIJPNK-GUBZILKMSA-N 0.000 description 2
- UFKPDBLKLOBMRH-XHNCKOQMSA-N Ser-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)N)C(=O)O UFKPDBLKLOBMRH-XHNCKOQMSA-N 0.000 description 2
- VQBCMLMPEWPUTB-ACZMJKKPSA-N Ser-Glu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O VQBCMLMPEWPUTB-ACZMJKKPSA-N 0.000 description 2
- GZBKRJVCRMZAST-XKBZYTNZSA-N Ser-Glu-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O GZBKRJVCRMZAST-XKBZYTNZSA-N 0.000 description 2
- WEQAYODCJHZSJZ-KKUMJFAQSA-N Ser-His-Tyr Chemical compound C([C@H](NC(=O)[C@H](CO)N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CN=CN1 WEQAYODCJHZSJZ-KKUMJFAQSA-N 0.000 description 2
- VMLONWHIORGALA-SRVKXCTJSA-N Ser-Leu-Leu Chemical compound CC(C)C[C@@H](C([O-])=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]([NH3+])CO VMLONWHIORGALA-SRVKXCTJSA-N 0.000 description 2
- GZSZPKSBVAOGIE-CIUDSAMLSA-N Ser-Lys-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O GZSZPKSBVAOGIE-CIUDSAMLSA-N 0.000 description 2
- XUDRHBPSPAPDJP-SRVKXCTJSA-N Ser-Lys-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CO XUDRHBPSPAPDJP-SRVKXCTJSA-N 0.000 description 2
- PTWIYDNFWPXQSD-GARJFASQSA-N Ser-Lys-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)N)C(=O)O PTWIYDNFWPXQSD-GARJFASQSA-N 0.000 description 2
- LRZLZIUXQBIWTB-KATARQTJSA-N Ser-Lys-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LRZLZIUXQBIWTB-KATARQTJSA-N 0.000 description 2
- WOJYIMBIKTWKJO-KKUMJFAQSA-N Ser-Phe-His Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)NC(=O)[C@H](CO)N WOJYIMBIKTWKJO-KKUMJFAQSA-N 0.000 description 2
- MQUZANJDFOQOBX-SRVKXCTJSA-N Ser-Phe-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(O)=O MQUZANJDFOQOBX-SRVKXCTJSA-N 0.000 description 2
- BSXKBOUZDAZXHE-CIUDSAMLSA-N Ser-Pro-Glu Chemical compound [H]N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O BSXKBOUZDAZXHE-CIUDSAMLSA-N 0.000 description 2
- PPCZVWHJWJFTFN-ZLUOBGJFSA-N Ser-Ser-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O PPCZVWHJWJFTFN-ZLUOBGJFSA-N 0.000 description 2
- JCLAFVNDBJMLBC-JBDRJPRFSA-N Ser-Ser-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O JCLAFVNDBJMLBC-JBDRJPRFSA-N 0.000 description 2
- BMKNXTJLHFIAAH-CIUDSAMLSA-N Ser-Ser-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O BMKNXTJLHFIAAH-CIUDSAMLSA-N 0.000 description 2
- OLKICIBQRVSQMA-SRVKXCTJSA-N Ser-Ser-Tyr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O OLKICIBQRVSQMA-SRVKXCTJSA-N 0.000 description 2
- VGQVAVQWKJLIRM-FXQIFTODSA-N Ser-Ser-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O VGQVAVQWKJLIRM-FXQIFTODSA-N 0.000 description 2
- SZRNDHWMVSFPSP-XKBZYTNZSA-N Ser-Thr-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CO)N)O SZRNDHWMVSFPSP-XKBZYTNZSA-N 0.000 description 2
- VLMIUSLQONKLDV-HEIBUPTGSA-N Ser-Thr-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O VLMIUSLQONKLDV-HEIBUPTGSA-N 0.000 description 2
- BDMWLJLPPUCLNV-XGEHTFHBSA-N Ser-Thr-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O BDMWLJLPPUCLNV-XGEHTFHBSA-N 0.000 description 2
- VVKVHAOOUGNDPJ-SRVKXCTJSA-N Ser-Tyr-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(O)=O VVKVHAOOUGNDPJ-SRVKXCTJSA-N 0.000 description 2
- PMTWIUBUQRGCSB-FXQIFTODSA-N Ser-Val-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(O)=O PMTWIUBUQRGCSB-FXQIFTODSA-N 0.000 description 2
- MFQMZDPAZRZAPV-NAKRPEOUSA-N Ser-Val-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CO)N MFQMZDPAZRZAPV-NAKRPEOUSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- VFEHSAJCWWHDBH-RHYQMDGZSA-N Thr-Arg-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(O)=O VFEHSAJCWWHDBH-RHYQMDGZSA-N 0.000 description 2
- OJRNZRROAIAHDL-LKXGYXEUSA-N Thr-Asn-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O OJRNZRROAIAHDL-LKXGYXEUSA-N 0.000 description 2
- JEDIEMIJYSRUBB-FOHZUACHSA-N Thr-Asp-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O JEDIEMIJYSRUBB-FOHZUACHSA-N 0.000 description 2
- APIQKJYZDWVOCE-VEVYYDQMSA-N Thr-Asp-Met Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCSC)C(O)=O APIQKJYZDWVOCE-VEVYYDQMSA-N 0.000 description 2
- UHBPFYOQQPFKQR-JHEQGTHGSA-N Thr-Gln-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O UHBPFYOQQPFKQR-JHEQGTHGSA-N 0.000 description 2
- DIPIPFHFLPTCLK-LOKLDPHHSA-N Thr-Gln-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@@H]1C(=O)O)N)O DIPIPFHFLPTCLK-LOKLDPHHSA-N 0.000 description 2
- CQNFRKAKGDSJFR-NUMRIWBASA-N Thr-Glu-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N)O CQNFRKAKGDSJFR-NUMRIWBASA-N 0.000 description 2
- GKWNLDNXMMLRMC-GLLZPBPUSA-N Thr-Glu-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O GKWNLDNXMMLRMC-GLLZPBPUSA-N 0.000 description 2
- SHOMROOOQBDGRL-JHEQGTHGSA-N Thr-Glu-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O SHOMROOOQBDGRL-JHEQGTHGSA-N 0.000 description 2
- ONNSECRQFSTMCC-XKBZYTNZSA-N Thr-Glu-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O ONNSECRQFSTMCC-XKBZYTNZSA-N 0.000 description 2
- XOTBWOCSLMBGMF-SUSMZKCASA-N Thr-Glu-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XOTBWOCSLMBGMF-SUSMZKCASA-N 0.000 description 2
- KCRQEJSKXAIULJ-FJXKBIBVSA-N Thr-Gly-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O KCRQEJSKXAIULJ-FJXKBIBVSA-N 0.000 description 2
- WYKJENSCCRJLRC-ZDLURKLDSA-N Thr-Gly-Cys Chemical compound C[C@H]([C@@H](C(=O)NCC(=O)N[C@@H](CS)C(=O)O)N)O WYKJENSCCRJLRC-ZDLURKLDSA-N 0.000 description 2
- VTVVYQOXJCZVEB-WDCWCFNPSA-N Thr-Leu-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O VTVVYQOXJCZVEB-WDCWCFNPSA-N 0.000 description 2
- FIFDDJFLNVAVMS-RHYQMDGZSA-N Thr-Leu-Met Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(O)=O FIFDDJFLNVAVMS-RHYQMDGZSA-N 0.000 description 2
- PZSDPRBZINDEJV-HTUGSXCWSA-N Thr-Phe-Gln Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(O)=O PZSDPRBZINDEJV-HTUGSXCWSA-N 0.000 description 2
- NZRUWPIYECBYRK-HTUGSXCWSA-N Thr-Phe-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(O)=O)C(O)=O NZRUWPIYECBYRK-HTUGSXCWSA-N 0.000 description 2
- BIBYEFRASCNLAA-CDMKHQONSA-N Thr-Phe-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CC1=CC=CC=C1 BIBYEFRASCNLAA-CDMKHQONSA-N 0.000 description 2
- GFRIEEKFXOVPIR-RHYQMDGZSA-N Thr-Pro-Lys Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(O)=O GFRIEEKFXOVPIR-RHYQMDGZSA-N 0.000 description 2
- KERCOYANYUPLHJ-XGEHTFHBSA-N Thr-Pro-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O KERCOYANYUPLHJ-XGEHTFHBSA-N 0.000 description 2
- GVMXJJAJLIEASL-ZJDVBMNYSA-N Thr-Pro-Thr Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O GVMXJJAJLIEASL-ZJDVBMNYSA-N 0.000 description 2
- FWTFAZKJORVTIR-VZFHVOOUSA-N Thr-Ser-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O FWTFAZKJORVTIR-VZFHVOOUSA-N 0.000 description 2
- PRTHQBSMXILLPC-XGEHTFHBSA-N Thr-Ser-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O PRTHQBSMXILLPC-XGEHTFHBSA-N 0.000 description 2
- NQQMWWVVGIXUOX-SVSWQMSJSA-N Thr-Ser-Ile Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O NQQMWWVVGIXUOX-SVSWQMSJSA-N 0.000 description 2
- NDZYTIMDOZMECO-SHGPDSBTSA-N Thr-Thr-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O NDZYTIMDOZMECO-SHGPDSBTSA-N 0.000 description 2
- ZESGVALRVJIVLZ-VFCFLDTKSA-N Thr-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@@H]1C(=O)O)N)O ZESGVALRVJIVLZ-VFCFLDTKSA-N 0.000 description 2
- KVEWWQRTAVMOFT-KJEVXHAQSA-N Thr-Tyr-Val Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(O)=O KVEWWQRTAVMOFT-KJEVXHAQSA-N 0.000 description 2
- MNYNCKZAEIAONY-XGEHTFHBSA-N Thr-Val-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O MNYNCKZAEIAONY-XGEHTFHBSA-N 0.000 description 2
- FKAPNDWDLDWZNF-QEJZJMRPSA-N Trp-Asp-Glu Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N FKAPNDWDLDWZNF-QEJZJMRPSA-N 0.000 description 2
- ORQGVWIUHICVKE-KCTSRDHCSA-N Trp-His-Ala Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(O)=O ORQGVWIUHICVKE-KCTSRDHCSA-N 0.000 description 2
- BEIGSKUPTIFYRZ-SRVKXCTJSA-N Tyr-Asp-Asp Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)O)N)O BEIGSKUPTIFYRZ-SRVKXCTJSA-N 0.000 description 2
- HIINQLBHPIQYHN-JTQLQIEISA-N Tyr-Gly-Gly Chemical compound OC(=O)CNC(=O)CNC(=O)[C@@H](N)CC1=CC=C(O)C=C1 HIINQLBHPIQYHN-JTQLQIEISA-N 0.000 description 2
- MVYRJYISVJWKSX-KBPBESRZSA-N Tyr-His-Gly Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC2=CN=CN2)C(=O)NCC(=O)O)N)O MVYRJYISVJWKSX-KBPBESRZSA-N 0.000 description 2
- UMSZZGTXGKHTFJ-SRVKXCTJSA-N Tyr-Ser-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 UMSZZGTXGKHTFJ-SRVKXCTJSA-N 0.000 description 2
- UUBKSZNKJUJQEJ-JRQIVUDYSA-N Tyr-Thr-Asp Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)N)O UUBKSZNKJUJQEJ-JRQIVUDYSA-N 0.000 description 2
- LTFLDDDGWOVIHY-NAKRPEOUSA-N Val-Ala-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](C)NC(=O)[C@H](C(C)C)N LTFLDDDGWOVIHY-NAKRPEOUSA-N 0.000 description 2
- AZSHAZJLOZQYAY-FXQIFTODSA-N Val-Ala-Ser Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O AZSHAZJLOZQYAY-FXQIFTODSA-N 0.000 description 2
- IVXJODPZRWHCCR-JYJNAYRXSA-N Val-Arg-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N IVXJODPZRWHCCR-JYJNAYRXSA-N 0.000 description 2
- VMRFIKXKOFNMHW-GUBZILKMSA-N Val-Arg-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(=O)O)N VMRFIKXKOFNMHW-GUBZILKMSA-N 0.000 description 2
- PVPAOIGJYHVWBT-KKHAAJSZSA-N Val-Asn-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](C(C)C)N)O PVPAOIGJYHVWBT-KKHAAJSZSA-N 0.000 description 2
- SCBITHMBEJNRHC-LSJOCFKGSA-N Val-Asp-Val Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)O)N SCBITHMBEJNRHC-LSJOCFKGSA-N 0.000 description 2
- VFOHXOLPLACADK-GVXVVHGQSA-N Val-Gln-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](C(C)C)N VFOHXOLPLACADK-GVXVVHGQSA-N 0.000 description 2
- FOADDSDHGRFUOC-DZKIICNBSA-N Val-Glu-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N FOADDSDHGRFUOC-DZKIICNBSA-N 0.000 description 2
- OQWNEUXPKHIEJO-NRPADANISA-N Val-Glu-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)O)N OQWNEUXPKHIEJO-NRPADANISA-N 0.000 description 2
- PMDOQZFYGWZSTK-LSJOCFKGSA-N Val-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)C(C)C PMDOQZFYGWZSTK-LSJOCFKGSA-N 0.000 description 2
- SYOMXKPPFZRELL-ONGXEEELSA-N Val-Gly-Lys Chemical compound CC(C)[C@@H](C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)O)N SYOMXKPPFZRELL-ONGXEEELSA-N 0.000 description 2
- ZTKGDWOUYRRAOQ-ULQDDVLXSA-N Val-His-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CC2=CC=CC=C2)C(=O)O)N ZTKGDWOUYRRAOQ-ULQDDVLXSA-N 0.000 description 2
- APQIVBCUIUDSMB-OSUNSFLBSA-N Val-Ile-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)O)NC(=O)[C@H](C(C)C)N APQIVBCUIUDSMB-OSUNSFLBSA-N 0.000 description 2
- BTWMICVCQLKKNR-DCAQKATOSA-N Val-Leu-Ser Chemical compound CC(C)[C@H]([NH3+])C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C([O-])=O BTWMICVCQLKKNR-DCAQKATOSA-N 0.000 description 2
- OJOMXGVLFKYDKP-QXEWZRGKSA-N Val-Met-Asp Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(=O)O)C(=O)O)N OJOMXGVLFKYDKP-QXEWZRGKSA-N 0.000 description 2
- CKTMJBPRVQWPHU-JSGCOSHPSA-N Val-Phe-Gly Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(=O)O)N CKTMJBPRVQWPHU-JSGCOSHPSA-N 0.000 description 2
- LGXUZJIQCGXKGZ-QXEWZRGKSA-N Val-Pro-Asn Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)N)C(=O)O)N LGXUZJIQCGXKGZ-QXEWZRGKSA-N 0.000 description 2
- DEGUERSKQBRZMZ-FXQIFTODSA-N Val-Ser-Ala Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O DEGUERSKQBRZMZ-FXQIFTODSA-N 0.000 description 2
- PZTZYZUTCPZWJH-FXQIFTODSA-N Val-Ser-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O)N PZTZYZUTCPZWJH-FXQIFTODSA-N 0.000 description 2
- UVHFONIHVHLDDQ-IFFSRLJSSA-N Val-Thr-Glu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N)O UVHFONIHVHLDDQ-IFFSRLJSSA-N 0.000 description 2
- CFIBZQOLUDURST-IHRRRGAJSA-N Val-Tyr-Cys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CS)C(=O)O)N CFIBZQOLUDURST-IHRRRGAJSA-N 0.000 description 2
- JSOXWWFKRJKTMT-WOPDTQHZSA-N Val-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N JSOXWWFKRJKTMT-WOPDTQHZSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 101710185494 Zinc finger protein Proteins 0.000 description 2
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 2
- 108010008685 alanyl-glutamyl-aspartic acid Proteins 0.000 description 2
- 108010041407 alanylaspartic acid Proteins 0.000 description 2
- 108010005233 alanylglutamic acid Proteins 0.000 description 2
- 108010044940 alanylglutamine Proteins 0.000 description 2
- 108010052670 arginyl-glutamyl-glutamic acid Proteins 0.000 description 2
- 108010069926 arginyl-glycyl-serine Proteins 0.000 description 2
- 108010062796 arginyllysine Proteins 0.000 description 2
- 108010066988 asparaginyl-alanyl-glycyl-alanine Proteins 0.000 description 2
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 2
- 108010093581 aspartyl-proline Proteins 0.000 description 2
- 108010047857 aspartylglycine Proteins 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000002962 chemical mutagen Substances 0.000 description 2
- 125000001309 chloro group Chemical group Cl* 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 108010025198 decaglycine Proteins 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 108010054812 diprotin A Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 108010078144 glutaminyl-glycine Proteins 0.000 description 2
- 108010008237 glutamyl-valyl-glycine Proteins 0.000 description 2
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 2
- 108010078326 glycyl-glycyl-valine Proteins 0.000 description 2
- 108010074027 glycyl-seryl-phenylalanine Proteins 0.000 description 2
- 108010010147 glycylglutamine Proteins 0.000 description 2
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
- 108010085325 histidylproline Proteins 0.000 description 2
- 108010018006 histidylserine Proteins 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 235000003642 hunger Nutrition 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 108010027338 isoleucylcysteine Proteins 0.000 description 2
- 108010000761 leucylarginine Proteins 0.000 description 2
- 108010057821 leucylproline Proteins 0.000 description 2
- 108010044348 lysyl-glutamyl-aspartic acid Proteins 0.000 description 2
- 108010009298 lysylglutamic acid Proteins 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 108010056582 methionylglutamic acid Proteins 0.000 description 2
- 108010085203 methionylmethionine Proteins 0.000 description 2
- 230000009456 molecular mechanism Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 235000012149 noodles Nutrition 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 108010051242 phenylalanylserine Proteins 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 108010014614 prolyl-glycyl-proline Proteins 0.000 description 2
- 108010007513 prolyl-glycyl-prolyl-leucine Proteins 0.000 description 2
- 108010077112 prolyl-proline Proteins 0.000 description 2
- 108010093296 prolyl-prolyl-alanine Proteins 0.000 description 2
- 108010079317 prolyl-tyrosine Proteins 0.000 description 2
- 108010004914 prolylarginine Proteins 0.000 description 2
- 108010015796 prolylisoleucine Proteins 0.000 description 2
- 108010090894 prolylleucine Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 108010048818 seryl-histidine Proteins 0.000 description 2
- 108010048397 seryl-lysyl-leucine Proteins 0.000 description 2
- 230000037351 starvation Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 108010071097 threonyl-lysyl-proline Proteins 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000005068 transpiration Effects 0.000 description 2
- 108010017949 tyrosyl-glycyl-glycine Proteins 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- PFOYSEIHFVKHNF-FXQIFTODSA-N Asn-Ala-Arg Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O PFOYSEIHFVKHNF-FXQIFTODSA-N 0.000 description 1
- HZPSDHRYYIORKR-WHFBIAKZSA-N Asn-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CC(N)=O HZPSDHRYYIORKR-WHFBIAKZSA-N 0.000 description 1
- 101710195281 Chlorophyll a-b binding protein Proteins 0.000 description 1
- 101710143415 Chlorophyll a-b binding protein 1, chloroplastic Proteins 0.000 description 1
- 101710181042 Chlorophyll a-b binding protein 1A, chloroplastic Proteins 0.000 description 1
- 101710091905 Chlorophyll a-b binding protein 2, chloroplastic Proteins 0.000 description 1
- 101710095244 Chlorophyll a-b binding protein 3, chloroplastic Proteins 0.000 description 1
- 101710127489 Chlorophyll a-b binding protein of LHCII type 1 Proteins 0.000 description 1
- 101710184917 Chlorophyll a-b binding protein of LHCII type I, chloroplastic Proteins 0.000 description 1
- 101710102593 Chlorophyll a-b binding protein, chloroplastic Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 102000005840 alpha-Galactosidase Human genes 0.000 description 1
- 108010030291 alpha-Galactosidase Proteins 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 235000019805 chlorophyllin Nutrition 0.000 description 1
- 229940099898 chlorophyllin Drugs 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 230000004034 genetic regulation Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 101150012864 ipt gene Proteins 0.000 description 1
- 230000011890 leaf development Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000029226 lipidation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- RARSHUDCJQSEFJ-UHFFFAOYSA-N p-Hydroxypropiophenone Chemical compound CCC(=O)C1=CC=C(O)C=C1 RARSHUDCJQSEFJ-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 235000019624 protein content Nutrition 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000015378 stomatal closure Effects 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8269—Photosynthesis
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明属于植物基因工程领域,具体涉及一种利用图位克隆技术克隆水稻ES1‑3基因,以及利用转基因互补试验鉴定基因的功能;同时还涉及该基因对水稻早衰调控的机理,用以改良农作物的光和效率,提高水稻产量。本发明公开了一种水稻早衰控制基因ES1‑3,本发明还同时公开了上述水稻早衰控制基因ES1‑3在调控水稻生育期和叶色中的应用。
Description
技术领域
本发明属于植物基因工程领域,具体涉及一种利用图位克隆技术克隆水稻ES1-3基因,以及利用转基因互补试验鉴定基因的功能;同时还涉及该基因对水稻早衰调控的机理,用以改良农作物的光和效率,提高水稻产量。
背景技术
水稻作为模式生物,同时又是世界上最主要的粮食作物,是植物功能基因组学研究的重要材料。水稻叶片衰老可导致光合能力的降低进而影响光合产物的形成和积累,因此,叶片早衰是水稻高产的主要限制因素之一[1]。当植物处于逆境时,为使种质得以延续,也会产生一种程序性细胞死亡的叶片衰老[2]。叶片早衰通常会导致水稻灌浆不足、结实率下降、千粒重变小等。在一些杂交稻组合中,功能叶过早衰老直接缩短了叶片的光合作用能力,导致光合产物积累的减少,进而影响水稻的产量。相反,延缓水稻叶片的衰老,则提高水稻产量[3]。过早的衰老对植物正常的生长发育会产生很大的负面影响,在水稻的生长过程中,早衰会严重限制水稻生育后期的生殖生长,是制约水稻高产潜力的发挥的重要因素之一,因此,通过对水稻叶片衰老相关基因的克隆和功能研究,揭示其遗传调控机制,对于培育和创制抗衰老水稻品种具有重要的意义。
一般来讲,叶片衰老总伴随着叶片颜色的变化,叶色的改变主要是由于叶片内的叶绿体含量发生了改变。叶片的细胞死亡是有序的,首先是细胞质收缩,此时核糖体在数量上急剧减少,然后核膜和液泡膜相继破裂,大量的水解酶进入细胞质内,导致死亡。植物衰老受很多信号的调节,这些信号有的是植物体内的激素等物质,有的是外部环境的胁迫或刺激,大部分信号对于PCD有同样的促进或抑制作用[4]。如CTK在延缓植物衰老的过程中的作用最大,主要是由于CTK参与了基因的表达调控,能在转录水平上调节RuBisco的表达,从而调节具有抑制衰老功能的光捕获蛋白LHCP 11的表达[5];赤霉素在植物的生长发育过程中能极大地促进茎、叶的生长,抑制叶片黄化。有学者对葡萄栽培叶片喷施外源的激素GA3,结果发现GA3处理过的叶片中,它们的叶绿素和蛋白质含量有所下降,叶片黄化延缓了,这证实GA对植物衰老有抑制作用;有研究发现,6-BA能够延迟叶片衰老,胡哲森(1998)设置了不同浓度梯度的6-BA用于处理油茶的叶片,发现油茶叶片的衰老明显推迟;多数情况下,IAA不是抑制衰老的重要因子,但有实验证明,生长素会引起细胞内一些物质的变化,如有些酶的活性会发生变化,叶绿素降解推迟,蛋白质水解、RNA降解等也和生长素有关系[6];相比之下,脱落酸和乙烯对植物叶片的衰老有促进作用,乙烯可通过加快SAGs(Senescence-associated genes)的表达,抑制光合基因的表达,从而促进衰老的形成。Grbic等学者用乙烯处理幼叶,结果发现SAGs的表达量没有明显变化,而用乙烯处理老叶,发现SAGs表达明显上升,并有衰老现象的出现[7]。ABA是仅次于乙烯的促衰剂,它主要是能诱导气孔关闭[8]。
随着现代分子手段的迅速发展,许多与衰老有关的基因已经被克隆出来。叶片衰老相关基因(Senescence-associated genes,SAGs)一般可划分为三类:第一类是下调基因,这类基因的mRNA丰度在衰老的叶片是明显降低的;第二类是衰老特异性很强的基因,这类基因仅在衰老期间被激活,其它时期一般不表达;第三类基因与第二类相似,不同的是这类基因在叶片发育早期就有一个较低的转录水平,衰老时,转录水平跳跃式升高[9]。如Os12编码的是GABA转氨基酶,而Osh69编码的是碱性α-半乳糖苷酶,这两个酶均是水稻叶片衰老时期的特异的表达产物,这两个基因水稻其它组织里不表达。GABA转氨基酶与脯氨酸代谢有密切关系,它是线粒体GABA支路的一个关键酶,此酶亦可防止ROS的堆积,所以在植物防御机制中也很重要[10];OsDos编码CCCH-型锌指蛋白,在叶片衰老和幼穗分化时期表达下降,将OsDos基因敲除后,植株在营养生长时期表现正常,没出现黄化现象,到了抽穗期后,叶片黄化加快,到灌浆时期,大部分叶片均已衰老,就剑叶还有一点绿色;ygl1也是一个表达下调的基因,其突变使得叶绿体合成的最后一步脂化反应受损,突变体幼苗时期叶片便开始黄化,不能正常生长发育[11];spl28突变体出现斑点叶,而且在开花后,植株的叶片开始迅速衰老,到开花后期枯萎,最后在成熟期死亡。
虽然已有的水稻早衰突变体和基因对叶片衰老分子机理的揭示起到了重要的作用,但是其分子机理有待进一步研究,
上文中涉及的参考文献如下:
[1]曹显祖,朱庆森.提高杂交稻结实率的中间试验.江苏农业科学,1981,18:5-6;
[2]Chae,H.,Lee,W.,Ethylene-and enzyme-mediated superoxide productionand cell death in carrot cells grown under carbon starvation.Plant CellReports 2002,20:256-261(Chae,H.,Lee,W.,在碳饥饿条件下生长的胡萝卜细胞中,乙烯和酶介导的超氧化物的产生和细胞死亡。植物细胞报告2002,20:56-261);
[3]王旭军,徐庆国,杨知建.水稻叶片衰老生理的研究进展.中国农学通报,2005,21:187-191;
[4]Thomas,H.,Ougham,H.J.,Wagstaff,C.,Stead,A.D.,Defining senescenceand death.Journal of experimental botany 2003,54:1127-1232(Thomas,H.,Ougham,H.J.,Wagstaff,C.,Stead,A.D.,定义衰老和死亡。实验植物学杂志2003,54:1127-1232);
[5]Robson,P.R.H.,Donnison,I.S.,Wang,K.,Frame,B.,Pegg,S.E.,Thomas,A.,Thomas,H.,Leaf senescence is delayed in maize expressing the AgrobacteriumIPT gene under the control of a novel maize senescence enhancedpromoter.Plant Biotechnology Journal 2004,2:101-112(Robson,P.R.H.,Donnison,I.S.,Wang,K.,Frame,B.,Pegg,S.E.,Thomas,A.,Thomas,H.,在一种新型的玉米衰老增强启动子的控制下,表达农杆菌IPT基因的玉米叶片衰老延迟。植物生物技术学报2004,2:101-112);
[6]包方,胡玉欣,李家洋.通过cDNA阵列技术鉴定拟南芥生长素应答基因.科学通报,2001,23:1988-1992;
[7]Grbi,V.,Bleecker,A.B.,Ethylene regulates the timing of leafsenescence in Arabidopsis.The Plant Journal 1995,8:595-602(Grbi,V.,Bleecker,A.B.,乙烯调节拟南芥叶片衰老的时间。植物学报1995,8:595-602);
[8]Noodle,L.D.,Guiam¨|t,J.J.,John,I.,Senescencemechanisms.Physiologia Plantarum1997,101:746-753(Noodle,L.D.,Guiam¨|t,J.J.,John,I.,衰老机制。植物生理学1997,101:746-753);
[9]严雯奕,叶胜海,董彦君,金庆生,张小明.植物叶片衰老相关研究进展.作物杂志,2010,4:4-9;
[10]Lee,R.H.,Wang,C.H.,Huang,L.T.,Chen,S.C.G.,Leaf senescence in riceplants:cloning and characterization of senescence up regulated genes.Journalof experimental botany 2001,52:1117-1123(Lee,R.H.,Wang,C.H.,Huang,L.T.,Chen,S.C.G.,水稻叶片衰老:衰老调控基因的克隆与表征;实验植物学杂志2001,52:1117-1123);
[11]Wu,Z.,Zhang,X.,He,B.,Diao,L.,Sheng,S.,Wang,J.,Guo,X.,Su,N.,Wang,L.,Jiang,L.,A chlorophyll-deficient rice mutant with impaired chlorophyllideesterification in chlorophyll biosynthesis.Plant Physiology,2007,107:100321-100322(Wu,Z.,Zhang,X.,He,B.,Diao,L.,Sheng,S.,Wang,J.,Guo,X.,Su,N.,Wang,L.,Jiang,L.,缺乏叶绿素的水稻突变体,叶绿素合成过程中叶绿素酯化作用受损。植物生理学,2007,107:100321-100322)。
发明内容
本发明要解决的技术问题是提供一种水稻叶片早衰控制基因ES1-3及其在水稻育种中的应用。
为了解决上述技术问题,本发明提供一种水稻叶片早衰控制基因ES1-3,该基因的核苷酸序列如SEQ ID NO:2所示。
本发明还同时提供了上述水稻叶片早衰控制基因ES1-3编码的蛋白质,该蛋白质的氨基酸序列如SEQ ID NO:4所示。
本发明还同时提供了上述水稻叶片早衰控制基因ES1-3在促进水稻早衰中的应用。
作为本发明的应用的改进,水稻早衰的表现为:水稻生育期缩短,叶色发黄,穗子变小,结实率降低。
本发明还同时提供了上述水稻早衰控制基因ES1-3在调控水稻叶色中的应用。
作为本发明的应用的改进:苗期叶片发黄明显,随着生长叶片颜色与野生型差异更加明显,可以作为遗传标记用于农业生产中的品种鉴定以及水稻遗传育种。
进一步地,上述氨基酸序列还添加、取代、插入或缺失一个或多个氨基酸或其他物种的同源序列而生成的氨基酸序列或衍生物。
进一步地,上述核苷酸序列还添加、取代、插入或缺失一个或多个核苷酸而生成的突变体、等位基因或衍生物。
本发明还提供了一种培育正常生育期水稻品种的方法,包括用上述植物表达载体转化植物细胞,再将转化的植物细胞培育成植株。转化可采用农杆菌介导法。
本发明的水稻早衰突变体可以与水稻晚熟品种杂交,改良晚熟品种的生育期。
本发明中的突变体材料水稻(Oryza sativa)早衰突变体es1-3(earlysenescence-number n)的原始野生型材料为日本晴,该突变体是利用化学诱变剂EMS(ethyl methane sulphonate)对粳稻品种日本晴处理后得到。该突变体苗期叶片在苗期就开始发黄,突变体在两个分蘖的时候,表型非常明显,新抽叶叶缘变黄,内卷,部分新叶呈螺旋状,老叶子上有点状锈斑。在体式镜下观察发现,野生型叶缘处有锯齿状,而在es1-3中却很平滑。
本发明采用图位克隆的方法克隆分离了水稻早衰基因ES1-3。ES1-3基因是由LOC_Os01g11040基因发生了单碱基缺失而来的,即序列SEQ ID NO:1的第4671位核苷酸G变成A,导致碱基序列发生改变,终止密码子提前出现,编码的蛋白质发生了改变,影响了维管束的发育,导致叶片表面硅质化颗粒的减少,因此出现叶色的改变;同时,该突变体缺少正常生长所需的能量,从而出现早衰现象。
通过转基因技术,进行功能互补的转基因研究,结果表明本发明获得了使突变体ES1-3的表型得以恢复为野生型的转基因水稻,证明了本发明正确克隆了ES1-3基因。
综上所述,本发明分离并克隆鉴定了控制水稻叶片早衰性状基因ES1-3,并通过互补实验进行基因功能验证。图位克隆的结果表明,该基因编码一个与水稻生育期相关的基因。本发明为培育叶色突变的早衰新品种,提供了一种培育高价值水稻突变体的方法。即,本发明通过图位克隆技术分离并克隆到早衰基因ES1-3,该基因编码一种与水稻生育期相关的基因,细胞学和生物化学分析表明,该基因影响水稻生育期、叶色、叶绿素含量、光合作用、分蘖数等,转基因功能互补实验鉴定了该基因的功能。
附图说明
下面结合附图对本发明的具体实施方式作进一步详细说明。
图1是野生型和es1-3突变体的表型图;
A:苗期表型;B:成熟期表型。
图2是ES1-3基因的定位图;
A:ES1-3基因的精细定位,利用es1-3/NIP的F2群体的183株突变单株,将ES1-3基因定位在第1号染色体的定位于C4和C7标记之间;
B:ES1-3界定在19.2Kb区域;
C:ES1-3基因的结构示意图,通过对该区域的NIP和es1-3亲本基因组DNA序列测序比对分析,结果发现在基因ES1-3(LOC_Os01g11040)的与第三内含子的剪切位点上的第4671位核苷酸G变成A,导致基因提前终止。
图3是野生型和es1-3突变体的穗部性状对比图;
A:倒四节节间长度;B:穗部籽粒数;C:籽粒性状
图4是野生型和es1-3突变体的叶绿素含量对比图;
图5是野生型和es1-3突变体的光合参数对比图。
具体实施方式
下面结合具体实施例对本发明进行进一步描述。这些描述并不是对本发明内容作进一步的限定,以下实施例中的若未特别说明,所用的技术手段为本领域技术人员所熟知的常规手段。
下述实施例中所用的材料、试剂等,如无特殊说明,均可从商业途径得到。
实施例1、突变体材料的获得
通过利用化学诱变剂EMS(ethyl methane sulphonate)对粳稻品种日本晴处理后得到,EMS处理方式为先用清水将待处理的日本晴干种子浸种8-10小时,之后沥干,再在28℃条件下,用浓度为1.5%的EMS溶液浸泡待处理种子10小时,用自来水冲洗10-12小时,最后放入到37℃烘箱内催芽。将处理后的日本晴进行自交,从而获得M2代。
突变体是在M2代发现的,后经多代自交,发现该突变体的表型能稳定遗传,并获得纯合的突变体株系。
从而筛选到一份早衰突变体es1-3。该突变体的性状经过多代自交已稳定遗传,在大田条件下观察和比较;突变体整个生长周期植株形态都表现出发黄的表型,且突变体叶缘平滑。所有水稻材料种植于浙江省金华市浙江师范大学生化学院试验田,常规管理。
实施例2、植株的表型分析
es1-3该突变体苗期叶片就开始发黄,随着生长叶片颜色与野生型差异更加明显,至抽穗时表现严重的早衰,以叶尖和叶缘尤甚(即,叶尖和叶缘颜色为枯黄色,叶尖卷曲)。另外抽穗时测量其节间长发现,es1-3的倒一节间长显著变短,穗子整体比野生型小很多,铁锈色的小斑点也可见于成熟后的籽粒,成熟的籽粒还伴有一定的裂颖,籽粒也略微变小,色泽变暗。
实施例3、叶绿素含量及光合速率的测定
利用便携式MIN LIA SPAD-502型叶绿素计测量野生型日本晴和突变体的叶片组织的叶绿素含量相对值,发现15个突变体的单株的测定平均叶绿素含量相对平均值达39.84,而野生型的测定的叶绿素含量相对平均值为42.65,差异显著。叶片的衰老一般会导致叶绿素含量的下降,叶色也会变得枯黄。为检测早衰突变体的叶绿素含量和野生型之间是否有差别,进一步采用丙酮法分别测定了突变体与野生型日本晴分蘖期功能叶片的叶绿素a、叶绿素b和总叶绿素含量,结果发现,如图4所示,突变体的叶绿素总量比日本晴显著降低,同时叶绿素a和叶绿素b的含量都减低了(P=0.05水平t测验差异达到极显著)。
叶绿素的降低可能会影响光合率的进行,为此,对同一时期的野生型和突变体的光合速率也做了检测,利用光合作用测定仪分别对日本晴和突变体的净光合速率、气孔导度、胞间CO2浓度及蒸腾速率等4项指标进行检测。如图5所示,结果发现早衰突变体的净光合效率、气孔导度、蒸腾速率都低于其野生型品种日本晴,而胞间CO2浓度则要高于对照野生型品种日本晴,说明突变体叶绿素含量的降低影响了其光合速率。
叶绿素含量测定方法:
田间选取分蘖盛期长势较一致的突变体植株和野生型植株日本晴的剑叶及倒2叶的新鲜叶片,剪成2mm左右的小片段,分别称取各个待测样品约0.20g,放入25ml的提取液(提取液由95%的丙酮与无水乙醇以2:1的体积比配制而成)中,在26℃黑暗条件下浸泡约24小时。用DU640分光光度计测量上清液分别在663.0nm(叶绿素a的最高吸收峰)、645.0nm(叶绿素b的最高吸收峰)和470.0nm(类胡萝卜素的最高吸收峰)这三个不同波长条件的光密度值,每个样品设置4个重复,取其平均值。然后根据Amon等(1949)的方法计算出各检测叶片中的叶绿素总量(chlorophyll)叶绿素a(Chl a)、叶绿素b(Chl b)和类胡萝卜素(Car)的含量,计算公式如表1所示。
表1叶绿素含量换算公式
其中:V为提取液体积(25ml),W为叶片质量,OD663、OD645及OD470为在分光光度仪上读取的光密度值,单位:mg/g。
实施例4、群体构建和遗传分析
将突变体es1-3和4个正常水稻品种(ZH11、CJ06、NJ6、TN1)进行正交和反交,所有F1植株均不表现早衰。F1植株均表现出正常野生型表型,说明es1-3受隐性核基因控制。将F1进行自交,得F2;统计F2分离群体分离比(表2),结果表明,正常表型的植株和突变体表型的植株的分离比经过卡方检验接近3:1分离,这表明es1-3的早衰表型是由一对单隐性核基因控制。
表2ES1-3和正常植株的F2群体遗传分析
实施例5、ES1-3基因的精细定位
利用本实验室保存的均匀分布于水稻12条染色体的相距10-30cM的164对SSR引物对突变体与NJ6进行多态性筛选。然后用320个es1-3/NJ6的F2中早衰单株进行连锁分析,初步确认目标基因所在的染色体位置。基因组DNA采用CTAB法提取。具体步骤如下:
①、称取0.1g的水稻叶片用液氮研磨成粉状,然后加入600μl的CTAB溶液(2%(m/V)CTAB,100mmol/L Tris-Cl,20mmol/L EDTA,1.4mol/L NaCl;pH8.0)配制的DNA提取缓冲液,65℃水浴40分钟。再加600μl的氯仿:异戊醇(24:1的体积比),并混匀。10,000rpm离心5分钟,将上清液转移到新的离心管中。
②、在上述步骤①离心后所得的上清液中加2/3~1体积倍的预冷(至4℃)的异丙醇,轻轻混匀至DNA沉淀。13,000rpm离心8分钟,倒出上清液。
③、再用70%(体积浓度)的已醇200μl洗涤上述步骤②所得的DNA沉淀物。
④、将上述洗涤后的DNA晾干并溶于100μl TE缓冲液或纯水中。
⑤、紫外分光光度法检测上述步骤④所得的DNA样品的浓度,0.7%的琼脂糖凝胶电泳检测DNA的完整性。完整合适的DNA用于PCR扩增,不完整的DNA则重新提取,直至获得完整的DNA。
PCR反应体系采用10μL体系:DNA模板1μL,10×PCR缓冲液1μL,正反向引物(10μmol/L)各0.5Μl,dNTPs 1μL,rTaq酶0.2μL,加ddH2O补足10μL。PCR扩增程序如下:94℃下预变性4min;94℃下变性30s,55℃~60℃下退火30s(温度因引物不同而异),72℃下延伸30s,40个循环;最后72℃下延伸10min。PCR产物用4%琼脂糖凝胶电泳,电泳结束后在凝胶成像仪拍照并读胶。利用上述筛选的164对SSR引物进行ES1-3基因连锁分析发现在第1号染色体的SSR标记M1处表现出连锁现象。在连锁标记上下游设计新的Indel标记,用这320个单株将目的基因区间锁定在分子标记M1与M2之间。在此区间再次设计新的分子标记,用320个F2单株最终将该基因定位在C4和C7之间大约19.2kb的区间内;引物序列见表3。
表3精细定位所用分子标记
根据水稻基因组数据库(http://rice.plantbiology.msu.edu/)数据信息,发现共有4个开放阅读框(ORF)。其中包括了1个表达蛋白,1个反转座子蛋白,0个功能蛋白。利用PCR的方法,将突变体和野生型中这6个基因所在的基因组序列扩增出来,测序分析,发现只有一个基因(LOC_Os01g11040)出现突变,该基因的编码区2146位核苷酸G变成A,导致编码的氨基酸发生改变,发生提前终止。
水稻早衰控制基因ES1-3的核苷酸序列为SEQ ID NO:2,水稻早衰控制基因ES1-3编码蛋白质的氨基酸序列为SEQ ID NO:4。
es1-3突变体对应的野生型日本晴(Oryza sativa)的核苷酸序列为SEQ ID NO:1;其码蛋白质的氨基酸序列如SEQ ID NO:3所述。
实施例6、植物转化
扩增早衰突变体es1-3中ES1-3基因的5’-UTR至3’-UTR的基因组DNA片段(SEQ IDNO:2),然后连入pEASY-Blunt Cloning Vector(TransGen Biotech公司)中,之后连入到pCAMBIA1300载体中;得pCAMBIA1300-COLD2。
质粒通过电击的方法转入农杆菌(pvgobacterium tumefaciens)株系EHA105中转化水稻。利用突变体成熟胚诱导的愈伤组织,经过诱导培养基培养2周后,挑选生长旺盛的愈伤用作转化的受体。用含有双元质粒载体(pCAMBIA1300-COLD2)的EHA105菌株侵染水稻愈伤,在黑暗、25℃条件下共培养3天后,在含有50mg/L Hygromycin的筛选培养基上光照培养14天左右(光照强度为13200LX,温度为32℃)。将预分化的愈伤转至分化培养基上在光照条件下(光照强度为13200LX,温度为32℃)培养一个月左右得到抗性转基因植株。对互补苗植株进行生育周期和叶片颜色鉴定,转入了早衰基因ES1-3的水稻其叶片颜色恢复正常,发育周期及抽穗数趋近野生型双科早品种。
最后,还需要注意的是,以上列举的仅是本发明的若干个具体实施例。显然,本发明不限于以上实施例,还可以有许多变形。本领域的普通技术人员能从本发明公开的内容直接导出或联想到的所有变形,均应认为是本发明的保护范围。
序列表
<110> 浙江师范大学
<120> 水稻早衰控制基因ES1-3及其应用
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 4005
<212> DNA
<213> 水稻(Oryza sativa)
<400> 1
atgccgctgg tgaggttcga ggtgcggaat gaggtggggc ttggggaccc cgacctgtac 60
ggcggcggcg gcggtggagg aggaggagga ggaggaggag gagttggggc tgctgcgaag 120
aagggcgggg aggcggagcc caaggcgctg ctcgagggcg tcgccgtcgc cggtctcgtc 180
gggatcctgc gccagctcgg agatctcgcg gaatttgcag cagatgtttt tcacgactta 240
catgagcaag ttataactac atctgctagg gggcgcaagg tgctgactcg agtacagaac 300
atcgaggcag cacttccatc tcttgaaaaa gctgtcaaga atcagaagag ccatatacat 360
ttcacttatg taccaggctc tgattggcat gcacaactta aagatgagca aaatcacctg 420
ctttctagtg atctacctcg atttatgatg gattcctatg aagaatgtcg agacccacca 480
cgactttacc ttcttgataa atttgataat gctggagctg gggcttgttc gaggagacat 540
tctgatccat catacttcaa gaaagcatgg gatatgatga gagcagacaa gacaggaaat 600
ttccaaagag aaaagaaatc tcagaaaatc aagagaaaag gatcacgctt gagagaaccg 660
tatcacggac aaactacacc caggcagagg aatggtgaat tgcagcgagc actcaccgct 720
gttcagctta ccagcaggca ctttgcgact cctagtactg atggccggag cctttcagag 780
aatagatcta catctgatgt aagatctaac cctgacaata taagcagatc ttcttcgttt 840
agttcgaaag cacgactgag tttcacagag caagttctag atacaaagcc aactgtagtt 900
cctcatgaaa atggccatga caagctgtca aataataatc tacacaagct tagcaatacc 960
cccttgcaca cacggcttaa cggtaccagt gcagatgacc tgggtgatga tttgaagcaa 1020
agttccctgc tagatgatat gactgctagg tcaccttctg ttaaatggga tgagaaggct 1080
gaaattacca tgtctacaac ttccgtctac tgtgatgatg ttgtcatgga caaggctgaa 1140
catgtacaat ctaaatgtat tagccctgag cagcaagaaa tcgaccatag ggagatggag 1200
actttggagc agcaagaggc attacatcaa aaggcaaaac agttattagt gtcatcaggc 1260
ttgaaccacc atgatgaagt ccccagtgaa acagacaact atgtggatgc acttaataca 1320
cttgaatctg agacagagac tgaacccgag cttcaaacta aaagtcgagt gaaaccagta 1380
ccttctctca atgttgatgt gcctcaggtg gagctgatag ataacattgt cacagagtct 1440
cctgattctt ctgttgctga attccctgat gcatatcaaa attcaagtat gcctcctgct 1500
cctgagagcg cagctgattt tcccagtttg tcaagtgcag atgctcctga catttcagag 1560
ccagtattat caggctatac agctaatcct catcctgaag tgtcagctat tgctactaat 1620
acccctgtga gcaatacaga ggatgctcca ggtcctttag agatttcaga gtcagcatca 1680
cgagcctata taattacact tcctaatcaa agtttacctg attccaaaga aattccagac 1740
agtaaggcag aagatgcacc catagattct cctgagaaat tggaaccagg accttcaagc 1800
tatacaccta caattcccat taaagagtcc tctattgtca gtcaaaatac taatgcagaa 1860
aatgtttccg gagactgtag tgaaggcact gcttgtgcta tatcctattc ccagcatatc 1920
atttctgata agccaactaa tgaggtatct gctactaata gctcacctga tgatacctct 1980
agcgatgaag atacagttga aagtggtggc attgttgaag tgtctaattc acagcccatg 2040
ccactgaatg actcattgga gaacggatgt gcaactcaag gcctcccagc aaatgcccct 2100
actaattcta ctggagtatc ttctgttaag ctctggacta atgctgggct ctttggactt 2160
gagccatcta aacctccagt attcggtgcc catgatggtc caaaggagga tactacacct 2220
ggacacacac aacctcagct ttgccattca actgggtgcc ccgaagttca tttttcaaag 2280
cccactgaat cagcacaagt atatgttcca aatggcaatt cgccaattac cagcagtttt 2340
gtggggaaac ttgttggtat ctgtcctggt tctacaagcc acagctcaga gactaatcaa 2400
tcaacagtaa gaacacctga tactgttatt ggtcaaacag aggggtccac aggttgttcc 2460
acatcttttg agcacagtga tcacaaaaat attattggta agcaaacttc aataagtgag 2520
ctcctagaat ctgaagacag tgctgaaaat ggtgctgaaa tgttctctaa aactgacatg 2580
actggaagga ataacatgaa tcaggtgtct gcatcaagct tttcaagcat tgcacaaaga 2640
tttcttgcta atacacttca gcgaagaact cccaaataca ctgatcttcc tatgtcatct 2700
gttatagtta acactgatgc aaacgggact gatgaatcta cccaaatatc ttctctagcc 2760
cccaatgaaa caacattcga ggcatctcaa tttgagaaga aaacagaaaa tgacacaaat 2820
ggactgccca aatcgtcact cttctctagt agccattact ctgagaaatc atctccgccg 2880
cttgagtaca tgaaaatatc tttccaccct atgagtgcat ttgaaatgtc aaaattggac 2940
ctagatttct ctgatgaaaa tcttcatgag aatgccgatg atatgatgtt accaacgttt 3000
cagttacttc cagggtcttc cgttccacag cttggtagtg gttctgaatc ggaagatgat 3060
acttttggca gatcttatag ttattcttcg tatgatgatc taagtccacg gttatattca 3120
aactctgagt tgtgggatca agaagacgca aatggattgg aggatcatga tatgcataac 3180
aatccaaatc agataggatc cttcggagca ccaatctcta gctttgtgga atttgagcag 3240
atggacttat ctggtgcgaa gtccactgta tcacttacag atcttgggga tgataatgga 3300
cttggcacgt tagattctca tcctgctgga gaacttccta acttcgatac tttgatggct 3360
catcaaaatg aggccttcat tccgcacaat ccagtaagtt tatcaccaga tgaaggtcag 3420
ttgcctccac ctcctcctct tcccccaatg caatggagga caatgagaca agtagcttct 3480
gtagaagaag gaagaggttc tgcagctaaa gaagatatgc ttgagagtac ctcagatcta 3540
ccaccagtac acactcctgt tcaggaagaa catcttctgc ccatcgcacc accagatcaa 3600
caaaatcttc tgcccatcgc accaccagat caacaagggc atgcgaagga gaatgacaga 3660
aaagttgatg gggtaaaaga gataagcaat cctctcgaca ttgagatcag agcaagcttg 3720
cttcagcaaa tcagggataa gtcaggtcag cagaagctga atggacatga aaagtcaaaa 3780
gcagtaggca atgatactaa aaacttggat gaaagggagg agttgcttca acaaatcagg 3840
agcaagacat tcaatttaag acgaacaaat gcatctaaga caaacacctc atcaccaacc 3900
actgccaact ccagcgttgt agcaatcttg gaaaaggcaa atgcaatccg ccaggctgtg 3960
gccagtgatg agggaggtga tgatgatagt tggagtgata tatga 4005
<210> 2
<211> 4005
<212> DNA
<213> 水稻(Oryza sativa)
<400> 2
atgccgctgg tgaggttcga ggtgcggaat gaggtggggc ttggggaccc cgacctgtac 60
ggcggcggcg gcggtggagg aggaggagga ggaggaggag gagttggggc tgctgcgaag 120
aagggcgggg aggcggagcc caaggcgctg ctcgagggcg tcgccgtcgc cggtctcgtc 180
gggatcctgc gccagctcgg agatctcgcg gaatttgcag cagatgtttt tcacgactta 240
catgagcaag ttataactac atctgctagg gggcgcaagg tgctgactcg agtacagaac 300
atcgaggcag cacttccatc tcttgaaaaa gctgtcaaga atcagaagag ccatatacat 360
ttcacttatg taccaggctc tgattggcat gcacaactta aagatgagca aaatcacctg 420
ctttctagtg atctacctcg atttatgatg gattcctatg aagaatgtcg agacccacca 480
cgactttacc ttcttgataa atttgataat gctggagctg gggcttgttc gaggagacat 540
tctgatccat catacttcaa gaaagcatgg gatatgatga gagcagacaa gacaggaaat 600
ttccaaagag aaaagaaatc tcagaaaatc aagagaaaag gatcacgctt gagagaaccg 660
tatcacggac aaactacacc caggcagagg aatggtgaat tgcagcgagc actcaccgct 720
gttcagctta ccagcaggca ctttgcgact cctagtactg atggccggag cctttcagag 780
aatagatcta catctgatgt aagatctaac cctgacaata taagcagatc ttcttcgttt 840
agttcgaaag cacgactgag tttcacagag caagttctag atacaaagcc aactgtagtt 900
cctcatgaaa atggccatga caagctgtca aataataatc tacacaagct tagcaatacc 960
cccttgcaca cacggcttaa cggtaccagt gcagatgacc tgggtgatga tttgaagcaa 1020
agttccctgc tagatgatat gactgctagg tcaccttctg ttaaatggga tgagaaggct 1080
gaaattacca tgtctacaac ttccgtctac tgtgatgatg ttgtcatgga caaggctgaa 1140
catgtacaat ctaaatgtat tagccctgag cagcaagaaa tcgaccatag ggagatggag 1200
actttggagc agcaagaggc attacatcaa aaggcaaaac agttattagt gtcatcaggc 1260
ttgaaccacc atgatgaagt ccccagtgaa acagacaact atgtggatgc acttaataca 1320
cttgaatctg agacagagac tgaacccgag cttcaaacta aaagtcgagt gaaaccagta 1380
ccttctctca atgttgatgt gcctcaggtg gagctgatag ataacattgt cacagagtct 1440
cctgattctt ctgttgctga attccctgat gcatatcaaa attcaagtat gcctcctgct 1500
cctgagagcg cagctgattt tcccagtttg tcaagtgcag atgctcctga catttcagag 1560
ccagtattat caggctatac agctaatcct catcctgaag tgtcagctat tgctactaat 1620
acccctgtga gcaatacaga ggatgctcca ggtcctttag agatttcaga gtcagcatca 1680
cgagcctata taattacact tcctaatcaa agtttacctg attccaaaga aattccagac 1740
agtaaggcag aagatgcacc catagattct cctgagaaat tggaaccagg accttcaagc 1800
tatacaccta caattcccat taaagagtcc tctattgtca gtcaaaatac taatgcagaa 1860
aatgtttccg gagactgtag tgaaggcact gcttgtgcta tatcctattc ccagcatatc 1920
atttctgata agccaactaa tgaggtatct gctactaata gctcacctga tgatacctct 1980
agcgatgaag atacagttga aagtggtggc attgttgaag tgtctaattc acagcccatg 2040
ccactgaatg actcattgga gaacggatgt gcaactcaag gcctcccagc aaatgcccct 2100
actaattcta ctggagtatc ttctgttaag ctctggacta atgctaggct ctttggactt 2160
gagccatcta aacctccagt attcggtgcc catgatggtc caaaggagga tactacacct 2220
ggacacacac aacctcagct ttgccattca actgggtgcc ccgaagttca tttttcaaag 2280
cccactgaat cagcacaagt atatgttcca aatggcaatt cgccaattac cagcagtttt 2340
gtggggaaac ttgttggtat ctgtcctggt tctacaagcc acagctcaga gactaatcaa 2400
tcaacagtaa gaacacctga tactgttatt ggtcaaacag aggggtccac aggttgttcc 2460
acatcttttg agcacagtga tcacaaaaat attattggta agcaaacttc aataagtgag 2520
ctcctagaat ctgaagacag tgctgaaaat ggtgctgaaa tgttctctaa aactgacatg 2580
actggaagga ataacatgaa tcaggtgtct gcatcaagct tttcaagcat tgcacaaaga 2640
tttcttgcta atacacttca gcgaagaact cccaaataca ctgatcttcc tatgtcatct 2700
gttatagtta acactgatgc aaacgggact gatgaatcta cccaaatatc ttctctagcc 2760
cccaatgaaa caacattcga ggcatctcaa tttgagaaga aaacagaaaa tgacacaaat 2820
ggactgccca aatcgtcact cttctctagt agccattact ctgagaaatc atctccgccg 2880
cttgagtaca tgaaaatatc tttccaccct atgagtgcat ttgaaatgtc aaaattggac 2940
ctagatttct ctgatgaaaa tcttcatgag aatgccgatg atatgatgtt accaacgttt 3000
cagttacttc cagggtcttc cgttccacag cttggtagtg gttctgaatc ggaagatgat 3060
acttttggca gatcttatag ttattcttcg tatgatgatc taagtccacg gttatattca 3120
aactctgagt tgtgggatca agaagacgca aatggattgg aggatcatga tatgcataac 3180
aatccaaatc agataggatc cttcggagca ccaatctcta gctttgtgga atttgagcag 3240
atggacttat ctggtgcgaa gtccactgta tcacttacag atcttgggga tgataatgga 3300
cttggcacgt tagattctca tcctgctgga gaacttccta acttcgatac tttgatggct 3360
catcaaaatg aggccttcat tccgcacaat ccagtaagtt tatcaccaga tgaaggtcag 3420
ttgcctccac ctcctcctct tcccccaatg caatggagga caatgagaca agtagcttct 3480
gtagaagaag gaagaggttc tgcagctaaa gaagatatgc ttgagagtac ctcagatcta 3540
ccaccagtac acactcctgt tcaggaagaa catcttctgc ccatcgcacc accagatcaa 3600
caaaatcttc tgcccatcgc accaccagat caacaagggc atgcgaagga gaatgacaga 3660
aaagttgatg gggtaaaaga gataagcaat cctctcgaca ttgagatcag agcaagcttg 3720
cttcagcaaa tcagggataa gtcaggtcag cagaagctga atggacatga aaagtcaaaa 3780
gcagtaggca atgatactaa aaacttggat gaaagggagg agttgcttca acaaatcagg 3840
agcaagacat tcaatttaag acgaacaaat gcatctaaga caaacacctc atcaccaacc 3900
actgccaact ccagcgttgt agcaatcttg gaaaaggcaa atgcaatccg ccaggctgtg 3960
gccagtgatg agggaggtga tgatgatagt tggagtgata tatga 4005
<210> 3
<211> 1334
<212> PRT
<213> 水稻(Oryza sativa)
<400> 3
Met Pro Leu Val Arg Phe Glu Val Arg Asn Glu Val Gly Leu Gly Asp
1 5 10 15
Pro Asp Leu Tyr Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly
20 25 30
Gly Gly Val Gly Ala Ala Ala Lys Lys Gly Gly Glu Ala Glu Pro Lys
35 40 45
Ala Leu Leu Glu Gly Val Ala Val Ala Gly Leu Val Gly Ile Leu Arg
50 55 60
Gln Leu Gly Asp Leu Ala Glu Phe Ala Ala Asp Val Phe His Asp Leu
65 70 75 80
His Glu Gln Val Ile Thr Thr Ser Ala Arg Gly Arg Lys Val Leu Thr
85 90 95
Arg Val Gln Asn Ile Glu Ala Ala Leu Pro Ser Leu Glu Lys Ala Val
100 105 110
Lys Asn Gln Lys Ser His Ile His Phe Thr Tyr Val Pro Gly Ser Asp
115 120 125
Trp His Ala Gln Leu Lys Asp Glu Gln Asn His Leu Leu Ser Ser Asp
130 135 140
Leu Pro Arg Phe Met Met Asp Ser Tyr Glu Glu Cys Arg Asp Pro Pro
145 150 155 160
Arg Leu Tyr Leu Leu Asp Lys Phe Asp Asn Ala Gly Ala Gly Ala Cys
165 170 175
Ser Arg Arg His Ser Asp Pro Ser Tyr Phe Lys Lys Ala Trp Asp Met
180 185 190
Met Arg Ala Asp Lys Thr Gly Asn Phe Gln Arg Glu Lys Lys Ser Gln
195 200 205
Lys Ile Lys Arg Lys Gly Ser Arg Leu Arg Glu Pro Tyr His Gly Gln
210 215 220
Thr Thr Pro Arg Gln Arg Asn Gly Glu Leu Gln Arg Ala Leu Thr Ala
225 230 235 240
Val Gln Leu Thr Ser Arg His Phe Ala Thr Pro Ser Thr Asp Gly Arg
245 250 255
Ser Leu Ser Glu Asn Arg Ser Thr Ser Asp Val Arg Ser Asn Pro Asp
260 265 270
Asn Ile Ser Arg Ser Ser Ser Phe Ser Ser Lys Ala Arg Leu Ser Phe
275 280 285
Thr Glu Gln Val Leu Asp Thr Lys Pro Thr Val Val Pro His Glu Asn
290 295 300
Gly His Asp Lys Leu Ser Asn Asn Asn Leu His Lys Leu Ser Asn Thr
305 310 315 320
Pro Leu His Thr Arg Leu Asn Gly Thr Ser Ala Asp Asp Leu Gly Asp
325 330 335
Asp Leu Lys Gln Ser Ser Leu Leu Asp Asp Met Thr Ala Arg Ser Pro
340 345 350
Ser Val Lys Trp Asp Glu Lys Ala Glu Ile Thr Met Ser Thr Thr Ser
355 360 365
Val Tyr Cys Asp Asp Val Val Met Asp Lys Ala Glu His Val Gln Ser
370 375 380
Lys Cys Ile Ser Pro Glu Gln Gln Glu Ile Asp His Arg Glu Met Glu
385 390 395 400
Thr Leu Glu Gln Gln Glu Ala Leu His Gln Lys Ala Lys Gln Leu Leu
405 410 415
Val Ser Ser Gly Leu Asn His His Asp Glu Val Pro Ser Glu Thr Asp
420 425 430
Asn Tyr Val Asp Ala Leu Asn Thr Leu Glu Ser Glu Thr Glu Thr Glu
435 440 445
Pro Glu Leu Gln Thr Lys Ser Arg Val Lys Pro Val Pro Ser Leu Asn
450 455 460
Val Asp Val Pro Gln Val Glu Leu Ile Asp Asn Ile Val Thr Glu Ser
465 470 475 480
Pro Asp Ser Ser Val Ala Glu Phe Pro Asp Ala Tyr Gln Asn Ser Ser
485 490 495
Met Pro Pro Ala Pro Glu Ser Ala Ala Asp Phe Pro Ser Leu Ser Ser
500 505 510
Ala Asp Ala Pro Asp Ile Ser Glu Pro Val Leu Ser Gly Tyr Thr Ala
515 520 525
Asn Pro His Pro Glu Val Ser Ala Ile Ala Thr Asn Thr Pro Val Ser
530 535 540
Asn Thr Glu Asp Ala Pro Gly Pro Leu Glu Ile Ser Glu Ser Ala Ser
545 550 555 560
Arg Ala Tyr Ile Ile Thr Leu Pro Asn Gln Ser Leu Pro Asp Ser Lys
565 570 575
Glu Ile Pro Asp Ser Lys Ala Glu Asp Ala Pro Ile Asp Ser Pro Glu
580 585 590
Lys Leu Glu Pro Gly Pro Ser Ser Tyr Thr Pro Thr Ile Pro Ile Lys
595 600 605
Glu Ser Ser Ile Val Ser Gln Asn Thr Asn Ala Glu Asn Val Ser Gly
610 615 620
Asp Cys Ser Glu Gly Thr Ala Cys Ala Ile Ser Tyr Ser Gln His Ile
625 630 635 640
Ile Ser Asp Lys Pro Thr Asn Glu Val Ser Ala Thr Asn Ser Ser Pro
645 650 655
Asp Asp Thr Ser Ser Asp Glu Asp Thr Val Glu Ser Gly Gly Ile Val
660 665 670
Glu Val Ser Asn Ser Gln Pro Met Pro Leu Asn Asp Ser Leu Glu Asn
675 680 685
Gly Cys Ala Thr Gln Gly Leu Pro Ala Asn Ala Pro Thr Asn Ser Thr
690 695 700
Gly Val Ser Ser Val Lys Leu Trp Thr Asn Ala Gly Leu Phe Gly Leu
705 710 715 720
Glu Pro Ser Lys Pro Pro Val Phe Gly Ala His Asp Gly Pro Lys Glu
725 730 735
Asp Thr Thr Pro Gly His Thr Gln Pro Gln Leu Cys His Ser Thr Gly
740 745 750
Cys Pro Glu Val His Phe Ser Lys Pro Thr Glu Ser Ala Gln Val Tyr
755 760 765
Val Pro Asn Gly Asn Ser Pro Ile Thr Ser Ser Phe Val Gly Lys Leu
770 775 780
Val Gly Ile Cys Pro Gly Ser Thr Ser His Ser Ser Glu Thr Asn Gln
785 790 795 800
Ser Thr Val Arg Thr Pro Asp Thr Val Ile Gly Gln Thr Glu Gly Ser
805 810 815
Thr Gly Cys Ser Thr Ser Phe Glu His Ser Asp His Lys Asn Ile Ile
820 825 830
Gly Lys Gln Thr Ser Ile Ser Glu Leu Leu Glu Ser Glu Asp Ser Ala
835 840 845
Glu Asn Gly Ala Glu Met Phe Ser Lys Thr Asp Met Thr Gly Arg Asn
850 855 860
Asn Met Asn Gln Val Ser Ala Ser Ser Phe Ser Ser Ile Ala Gln Arg
865 870 875 880
Phe Leu Ala Asn Thr Leu Gln Arg Arg Thr Pro Lys Tyr Thr Asp Leu
885 890 895
Pro Met Ser Ser Val Ile Val Asn Thr Asp Ala Asn Gly Thr Asp Glu
900 905 910
Ser Thr Gln Ile Ser Ser Leu Ala Pro Asn Glu Thr Thr Phe Glu Ala
915 920 925
Ser Gln Phe Glu Lys Lys Thr Glu Asn Asp Thr Asn Gly Leu Pro Lys
930 935 940
Ser Ser Leu Phe Ser Ser Ser His Tyr Ser Glu Lys Ser Ser Pro Pro
945 950 955 960
Leu Glu Tyr Met Lys Ile Ser Phe His Pro Met Ser Ala Phe Glu Met
965 970 975
Ser Lys Leu Asp Leu Asp Phe Ser Asp Glu Asn Leu His Glu Asn Ala
980 985 990
Asp Asp Met Met Leu Pro Thr Phe Gln Leu Leu Pro Gly Ser Ser Val
995 1000 1005
Pro Gln Leu Gly Ser Gly Ser Glu Ser Glu Asp Asp Thr Phe Gly Arg
1010 1015 1020
Ser Tyr Ser Tyr Ser Ser Tyr Asp Asp Leu Ser Pro Arg Leu Tyr Ser
1025 1030 1035 1040
Asn Ser Glu Leu Trp Asp Gln Glu Asp Ala Asn Gly Leu Glu Asp His
1045 1050 1055
Asp Met His Asn Asn Pro Asn Gln Ile Gly Ser Phe Gly Ala Pro Ile
1060 1065 1070
Ser Ser Phe Val Glu Phe Glu Gln Met Asp Leu Ser Gly Ala Lys Ser
1075 1080 1085
Thr Val Ser Leu Thr Asp Leu Gly Asp Asp Asn Gly Leu Gly Thr Leu
1090 1095 1100
Asp Ser His Pro Ala Gly Glu Leu Pro Asn Phe Asp Thr Leu Met Ala
1105 1110 1115 1120
His Gln Asn Glu Ala Phe Ile Pro His Asn Pro Val Ser Leu Ser Pro
1125 1130 1135
Asp Glu Gly Gln Leu Pro Pro Pro Pro Pro Leu Pro Pro Met Gln Trp
1140 1145 1150
Arg Thr Met Arg Gln Val Ala Ser Val Glu Glu Gly Arg Gly Ser Ala
1155 1160 1165
Ala Lys Glu Asp Met Leu Glu Ser Thr Ser Asp Leu Pro Pro Val His
1170 1175 1180
Thr Pro Val Gln Glu Glu His Leu Leu Pro Ile Ala Pro Pro Asp Gln
1185 1190 1195 1200
Gln Asn Leu Leu Pro Ile Ala Pro Pro Asp Gln Gln Gly His Ala Lys
1205 1210 1215
Glu Asn Asp Arg Lys Val Asp Gly Val Lys Glu Ile Ser Asn Pro Leu
1220 1225 1230
Asp Ile Glu Ile Arg Ala Ser Leu Leu Gln Gln Ile Arg Asp Lys Ser
1235 1240 1245
Gly Gln Gln Lys Leu Asn Gly His Glu Lys Ser Lys Ala Val Gly Asn
1250 1255 1260
Asp Thr Lys Asn Leu Asp Glu Arg Glu Glu Leu Leu Gln Gln Ile Arg
1265 1270 1275 1280
Ser Lys Thr Phe Asn Leu Arg Arg Thr Asn Ala Ser Lys Thr Asn Thr
1285 1290 1295
Ser Ser Pro Thr Thr Ala Asn Ser Ser Val Val Ala Ile Leu Glu Lys
1300 1305 1310
Ala Asn Ala Ile Arg Gln Ala Val Ala Ser Asp Glu Gly Gly Asp Asp
1315 1320 1325
Asp Ser Trp Ser Asp Ile
1330
<210> 4
<211> 1334
<212> PRT
<213> 水稻(Oryza sativa)
<400> 4
Met Pro Leu Val Arg Phe Glu Val Arg Asn Glu Val Gly Leu Gly Asp
1 5 10 15
Pro Asp Leu Tyr Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly
20 25 30
Gly Gly Val Gly Ala Ala Ala Lys Lys Gly Gly Glu Ala Glu Pro Lys
35 40 45
Ala Leu Leu Glu Gly Val Ala Val Ala Gly Leu Val Gly Ile Leu Arg
50 55 60
Gln Leu Gly Asp Leu Ala Glu Phe Ala Ala Asp Val Phe His Asp Leu
65 70 75 80
His Glu Gln Val Ile Thr Thr Ser Ala Arg Gly Arg Lys Val Leu Thr
85 90 95
Arg Val Gln Asn Ile Glu Ala Ala Leu Pro Ser Leu Glu Lys Ala Val
100 105 110
Lys Asn Gln Lys Ser His Ile His Phe Thr Tyr Val Pro Gly Ser Asp
115 120 125
Trp His Ala Gln Leu Lys Asp Glu Gln Asn His Leu Leu Ser Ser Asp
130 135 140
Leu Pro Arg Phe Met Met Asp Ser Tyr Glu Glu Cys Arg Asp Pro Pro
145 150 155 160
Arg Leu Tyr Leu Leu Asp Lys Phe Asp Asn Ala Gly Ala Gly Ala Cys
165 170 175
Ser Arg Arg His Ser Asp Pro Ser Tyr Phe Lys Lys Ala Trp Asp Met
180 185 190
Met Arg Ala Asp Lys Thr Gly Asn Phe Gln Arg Glu Lys Lys Ser Gln
195 200 205
Lys Ile Lys Arg Lys Gly Ser Arg Leu Arg Glu Pro Tyr His Gly Gln
210 215 220
Thr Thr Pro Arg Gln Arg Asn Gly Glu Leu Gln Arg Ala Leu Thr Ala
225 230 235 240
Val Gln Leu Thr Ser Arg His Phe Ala Thr Pro Ser Thr Asp Gly Arg
245 250 255
Ser Leu Ser Glu Asn Arg Ser Thr Ser Asp Val Arg Ser Asn Pro Asp
260 265 270
Asn Ile Ser Arg Ser Ser Ser Phe Ser Ser Lys Ala Arg Leu Ser Phe
275 280 285
Thr Glu Gln Val Leu Asp Thr Lys Pro Thr Val Val Pro His Glu Asn
290 295 300
Gly His Asp Lys Leu Ser Asn Asn Asn Leu His Lys Leu Ser Asn Thr
305 310 315 320
Pro Leu His Thr Arg Leu Asn Gly Thr Ser Ala Asp Asp Leu Gly Asp
325 330 335
Asp Leu Lys Gln Ser Ser Leu Leu Asp Asp Met Thr Ala Arg Ser Pro
340 345 350
Ser Val Lys Trp Asp Glu Lys Ala Glu Ile Thr Met Ser Thr Thr Ser
355 360 365
Val Tyr Cys Asp Asp Val Val Met Asp Lys Ala Glu His Val Gln Ser
370 375 380
Lys Cys Ile Ser Pro Glu Gln Gln Glu Ile Asp His Arg Glu Met Glu
385 390 395 400
Thr Leu Glu Gln Gln Glu Ala Leu His Gln Lys Ala Lys Gln Leu Leu
405 410 415
Val Ser Ser Gly Leu Asn His His Asp Glu Val Pro Ser Glu Thr Asp
420 425 430
Asn Tyr Val Asp Ala Leu Asn Thr Leu Glu Ser Glu Thr Glu Thr Glu
435 440 445
Pro Glu Leu Gln Thr Lys Ser Arg Val Lys Pro Val Pro Ser Leu Asn
450 455 460
Val Asp Val Pro Gln Val Glu Leu Ile Asp Asn Ile Val Thr Glu Ser
465 470 475 480
Pro Asp Ser Ser Val Ala Glu Phe Pro Asp Ala Tyr Gln Asn Ser Ser
485 490 495
Met Pro Pro Ala Pro Glu Ser Ala Ala Asp Phe Pro Ser Leu Ser Ser
500 505 510
Ala Asp Ala Pro Asp Ile Ser Glu Pro Val Leu Ser Gly Tyr Thr Ala
515 520 525
Asn Pro His Pro Glu Val Ser Ala Ile Ala Thr Asn Thr Pro Val Ser
530 535 540
Asn Thr Glu Asp Ala Pro Gly Pro Leu Glu Ile Ser Glu Ser Ala Ser
545 550 555 560
Arg Ala Tyr Ile Ile Thr Leu Pro Asn Gln Ser Leu Pro Asp Ser Lys
565 570 575
Glu Ile Pro Asp Ser Lys Ala Glu Asp Ala Pro Ile Asp Ser Pro Glu
580 585 590
Lys Leu Glu Pro Gly Pro Ser Ser Tyr Thr Pro Thr Ile Pro Ile Lys
595 600 605
Glu Ser Ser Ile Val Ser Gln Asn Thr Asn Ala Glu Asn Val Ser Gly
610 615 620
Asp Cys Ser Glu Gly Thr Ala Cys Ala Ile Ser Tyr Ser Gln His Ile
625 630 635 640
Ile Ser Asp Lys Pro Thr Asn Glu Val Ser Ala Thr Asn Ser Ser Pro
645 650 655
Asp Asp Thr Ser Ser Asp Glu Asp Thr Val Glu Ser Gly Gly Ile Val
660 665 670
Glu Val Ser Asn Ser Gln Pro Met Pro Leu Asn Asp Ser Leu Glu Asn
675 680 685
Gly Cys Ala Thr Gln Gly Leu Pro Ala Asn Ala Pro Thr Asn Ser Thr
690 695 700
Gly Val Ser Ser Val Lys Leu Trp Thr Asn Ala Arg Leu Phe Gly Leu
705 710 715 720
Glu Pro Ser Lys Pro Pro Val Phe Gly Ala His Asp Gly Pro Lys Glu
725 730 735
Asp Thr Thr Pro Gly His Thr Gln Pro Gln Leu Cys His Ser Thr Gly
740 745 750
Cys Pro Glu Val His Phe Ser Lys Pro Thr Glu Ser Ala Gln Val Tyr
755 760 765
Val Pro Asn Gly Asn Ser Pro Ile Thr Ser Ser Phe Val Gly Lys Leu
770 775 780
Val Gly Ile Cys Pro Gly Ser Thr Ser His Ser Ser Glu Thr Asn Gln
785 790 795 800
Ser Thr Val Arg Thr Pro Asp Thr Val Ile Gly Gln Thr Glu Gly Ser
805 810 815
Thr Gly Cys Ser Thr Ser Phe Glu His Ser Asp His Lys Asn Ile Ile
820 825 830
Gly Lys Gln Thr Ser Ile Ser Glu Leu Leu Glu Ser Glu Asp Ser Ala
835 840 845
Glu Asn Gly Ala Glu Met Phe Ser Lys Thr Asp Met Thr Gly Arg Asn
850 855 860
Asn Met Asn Gln Val Ser Ala Ser Ser Phe Ser Ser Ile Ala Gln Arg
865 870 875 880
Phe Leu Ala Asn Thr Leu Gln Arg Arg Thr Pro Lys Tyr Thr Asp Leu
885 890 895
Pro Met Ser Ser Val Ile Val Asn Thr Asp Ala Asn Gly Thr Asp Glu
900 905 910
Ser Thr Gln Ile Ser Ser Leu Ala Pro Asn Glu Thr Thr Phe Glu Ala
915 920 925
Ser Gln Phe Glu Lys Lys Thr Glu Asn Asp Thr Asn Gly Leu Pro Lys
930 935 940
Ser Ser Leu Phe Ser Ser Ser His Tyr Ser Glu Lys Ser Ser Pro Pro
945 950 955 960
Leu Glu Tyr Met Lys Ile Ser Phe His Pro Met Ser Ala Phe Glu Met
965 970 975
Ser Lys Leu Asp Leu Asp Phe Ser Asp Glu Asn Leu His Glu Asn Ala
980 985 990
Asp Asp Met Met Leu Pro Thr Phe Gln Leu Leu Pro Gly Ser Ser Val
995 1000 1005
Pro Gln Leu Gly Ser Gly Ser Glu Ser Glu Asp Asp Thr Phe Gly Arg
1010 1015 1020
Ser Tyr Ser Tyr Ser Ser Tyr Asp Asp Leu Ser Pro Arg Leu Tyr Ser
1025 1030 1035 1040
Asn Ser Glu Leu Trp Asp Gln Glu Asp Ala Asn Gly Leu Glu Asp His
1045 1050 1055
Asp Met His Asn Asn Pro Asn Gln Ile Gly Ser Phe Gly Ala Pro Ile
1060 1065 1070
Ser Ser Phe Val Glu Phe Glu Gln Met Asp Leu Ser Gly Ala Lys Ser
1075 1080 1085
Thr Val Ser Leu Thr Asp Leu Gly Asp Asp Asn Gly Leu Gly Thr Leu
1090 1095 1100
Asp Ser His Pro Ala Gly Glu Leu Pro Asn Phe Asp Thr Leu Met Ala
1105 1110 1115 1120
His Gln Asn Glu Ala Phe Ile Pro His Asn Pro Val Ser Leu Ser Pro
1125 1130 1135
Asp Glu Gly Gln Leu Pro Pro Pro Pro Pro Leu Pro Pro Met Gln Trp
1140 1145 1150
Arg Thr Met Arg Gln Val Ala Ser Val Glu Glu Gly Arg Gly Ser Ala
1155 1160 1165
Ala Lys Glu Asp Met Leu Glu Ser Thr Ser Asp Leu Pro Pro Val His
1170 1175 1180
Thr Pro Val Gln Glu Glu His Leu Leu Pro Ile Ala Pro Pro Asp Gln
1185 1190 1195 1200
Gln Asn Leu Leu Pro Ile Ala Pro Pro Asp Gln Gln Gly His Ala Lys
1205 1210 1215
Glu Asn Asp Arg Lys Val Asp Gly Val Lys Glu Ile Ser Asn Pro Leu
1220 1225 1230
Asp Ile Glu Ile Arg Ala Ser Leu Leu Gln Gln Ile Arg Asp Lys Ser
1235 1240 1245
Gly Gln Gln Lys Leu Asn Gly His Glu Lys Ser Lys Ala Val Gly Asn
1250 1255 1260
Asp Thr Lys Asn Leu Asp Glu Arg Glu Glu Leu Leu Gln Gln Ile Arg
1265 1270 1275 1280
Ser Lys Thr Phe Asn Leu Arg Arg Thr Asn Ala Ser Lys Thr Asn Thr
1285 1290 1295
Ser Ser Pro Thr Thr Ala Asn Ser Ser Val Val Ala Ile Leu Glu Lys
1300 1305 1310
Ala Asn Ala Ile Arg Gln Ala Val Ala Ser Asp Glu Gly Gly Asp Asp
1315 1320 1325
Asp Ser Trp Ser Asp Ile
1330
Claims (6)
1.水稻早衰控制基因ES1-3,其特征是:所述基因的核苷酸序列如SEQ ID NO:2所示。
2.如权利要求1所述的水稻早衰控制基因ES1-3编码的蛋白质,其特征是:所述蛋白质的氨基酸序列如SEQ ID NO:4所示。
3.如权利要求1所述水稻早衰控制基因ES1-3在促进水稻早衰中的应用。
4.根据权利要求3所述的应用,其特征在于,水稻早衰的表现为:水稻生育周期缩短,叶色发黄,穗子变小,结实率降低。
5.如权利要求1所述水稻早衰控制基因ES1-3在调控水稻生育期和叶色中的应用。
6.根据权利要求5所述的应用,其特征在于:苗期叶片发黄明显,随着生长叶片颜色与野生型差异明显,作为遗传标记用于农业生产中的品种鉴定以及水稻遗传育种。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011626237.2A CN112592393B (zh) | 2020-12-31 | 2020-12-31 | 水稻早衰控制基因es1-3及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011626237.2A CN112592393B (zh) | 2020-12-31 | 2020-12-31 | 水稻早衰控制基因es1-3及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN112592393A true CN112592393A (zh) | 2021-04-02 |
CN112592393B CN112592393B (zh) | 2024-02-20 |
Family
ID=75206658
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011626237.2A Active CN112592393B (zh) | 2020-12-31 | 2020-12-31 | 水稻早衰控制基因es1-3及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112592393B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112941083A (zh) * | 2021-02-25 | 2021-06-11 | 杭州师范大学 | 一种水稻类病变衰老调控基因及应用 |
CN113699163A (zh) * | 2021-05-31 | 2021-11-26 | 浙江师范大学 | 水稻早衰矮化基因esd1及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101686641A (zh) * | 2007-02-05 | 2010-03-31 | 新加坡国立大学 | 推定的细胞激动素受体和其应用方法 |
CN106967736A (zh) * | 2017-04-10 | 2017-07-21 | 中国水稻研究所 | 水稻OsMts1基因及其编码蛋白与应用 |
CN108504772A (zh) * | 2018-06-05 | 2018-09-07 | 浙江农林大学 | 水稻早衰基因的分子标记及应用 |
CN109234286A (zh) * | 2018-10-22 | 2019-01-18 | 杭州师范大学 | 一种水稻叶片衰老调控基因els6及其编码的蛋白质和应用 |
-
2020
- 2020-12-31 CN CN202011626237.2A patent/CN112592393B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101686641A (zh) * | 2007-02-05 | 2010-03-31 | 新加坡国立大学 | 推定的细胞激动素受体和其应用方法 |
CN106967736A (zh) * | 2017-04-10 | 2017-07-21 | 中国水稻研究所 | 水稻OsMts1基因及其编码蛋白与应用 |
CN108504772A (zh) * | 2018-06-05 | 2018-09-07 | 浙江农林大学 | 水稻早衰基因的分子标记及应用 |
CN109234286A (zh) * | 2018-10-22 | 2019-01-18 | 杭州师范大学 | 一种水稻叶片衰老调控基因els6及其编码的蛋白质和应用 |
Non-Patent Citations (8)
Title |
---|
RAO Y等: "EARLY SENESCENCE1 Encodes a SCAR-LIKE PROTEIN2 That Affects Water Loss in Rice", 《PLANT PHYSIOLOGY》 * |
RAO Y等: "EARLY SENESCENCE1 Encodes a SCAR-LIKE PROTEIN2 That Affects Water Loss in Rice", 《PLANT PHYSIOLOGY》, vol. 169, no. 2, 4 August 2015 (2015-08-04), pages 1227 - 1228 * |
徐娜等: "水稻叶片早衰成因及分子机理研究进展", 《植物学报》 * |
徐娜等: "水稻叶片早衰成因及分子机理研究进展", 《植物学报》, vol. 52, no. 01, 14 February 2017 (2017-02-14), pages 102 - 112 * |
杨窑龙DENG: "水稻早衰叶突变体es-t 的遗传分析与精细定位", 《中国科学》 * |
杨窑龙DENG: "水稻早衰叶突变体es-t 的遗传分析与精细定位", 《中国科学》, vol. 56, no. 19, 15 July 2011 (2011-07-15), pages 1539 - 1545 * |
饶玉春: "早衰基因ES1影响水稻失水", 《中国农业科学院博士后研究工作报告》 * |
饶玉春: "早衰基因ES1影响水稻失水", 《中国农业科学院博士后研究工作报告》, 15 March 2017 (2017-03-15), pages 15 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112941083A (zh) * | 2021-02-25 | 2021-06-11 | 杭州师范大学 | 一种水稻类病变衰老调控基因及应用 |
CN112941083B (zh) * | 2021-02-25 | 2022-12-06 | 杭州师范大学 | 一种水稻类病变衰老调控基因及应用 |
CN113699163A (zh) * | 2021-05-31 | 2021-11-26 | 浙江师范大学 | 水稻早衰矮化基因esd1及其应用 |
CN113699163B (zh) * | 2021-05-31 | 2023-10-03 | 浙江师范大学 | 水稻早衰矮化基因esd1及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN112592393B (zh) | 2024-02-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9062323B2 (en) | Identification and use of KRP mutants in wheat | |
CN111778265B (zh) | 玉米赤霉素氧化酶的突变基因、突变体、表达载体和应用 | |
WO2005079168A2 (en) | A novel stay-green gene and method for preparing stay-green transgenic plants | |
US20040088763A1 (en) | Gene hd3a inducing flowering of plant and utilization thereof | |
US20150299723A1 (en) | Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (llrk) polypeptides and homologs thereof | |
CN112592393B (zh) | 水稻早衰控制基因es1-3及其应用 | |
CN112301039A (zh) | 一种玉米叶斑马条纹叶色基因zb9以及与其连锁的InDel分子标记和应用 | |
CN108570474B (zh) | 水稻花发育基因eh1及其应用 | |
CN113004383B (zh) | 玉米基因ZmEREB102在提高玉米产量中的应用 | |
CN108003227B (zh) | 一种水稻早花时相关蛋白及其编码基因 | |
CN112175973B (zh) | 水稻类病斑控制基因spl36及其应用 | |
CN109251932A (zh) | 水稻早熟鲜绿控制基因pe-1及其应用 | |
CN111511916A (zh) | 花期调控基因cmp1和相关的载体及其应用 | |
Ozawa et al. | Enhancement of regeneration of rice (Oryza sativa L.) calli by integration of the gene involved in regeneration ability of the callus | |
CN114836441B (zh) | 玉米种子耐贮性相关的ZmRAP2.7基因及其功能标记与应用 | |
US20110277183A1 (en) | Alteration of plant architecture characteristics in plants | |
CN106967734B (zh) | 水稻矮化小穗基因dsp1及其应用 | |
CN112626085A (zh) | 水稻窄叶基因nal13及其应用 | |
CN109456984B (zh) | 水稻颖壳发育基因ah1及其应用 | |
CN112195162A (zh) | 水稻叶片衰老控制基因es2及其应用 | |
CN109988754A (zh) | 一种水稻蜡质合成相关的蛋白质及其编码基因wsl5与应用 | |
CN113308448B (zh) | 一种水稻叶色调控基因wss1及其编码蛋白质和应用 | |
CN113817754B (zh) | 水稻短粒基因shg1及其应用 | |
NL2030997B1 (en) | Zea mays receptor-like kinase 7 (zmrlk7) gene related to kernel and plant type development of maize and use thereof | |
CN111826393B (zh) | 一种同时调控水稻粒型粒重和抗性的基因及其编码蛋白的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |