CN112570154B - Centrifugal liquid changing device - Google Patents
Centrifugal liquid changing device Download PDFInfo
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- CN112570154B CN112570154B CN202011403871.XA CN202011403871A CN112570154B CN 112570154 B CN112570154 B CN 112570154B CN 202011403871 A CN202011403871 A CN 202011403871A CN 112570154 B CN112570154 B CN 112570154B
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- centrifugal
- pipeline
- tube
- way joint
- shell
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- 239000007788 liquid Substances 0.000 title claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 36
- 238000007789 sealing Methods 0.000 claims abstract description 19
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- 238000011084 recovery Methods 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000309459 oncolytic virus Species 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/02—Electric motor drives
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/16—Sealings between relatively-moving surfaces
Abstract
The invention relates to the technical field of bioreactors, in particular to a centrifugal liquid exchange device which comprises a centrifugal mechanism, a supernatant recovery mechanism and a reaction tank, wherein the centrifugal mechanism consists of a shell, a centrifugal barrel and a base, the lower end of the shell is provided with the base, the centrifugal barrel is arranged in the shell and the base, the upper end of the centrifugal barrel is provided with a multi-way joint, a first pipeline, a second pipeline and a third pipeline are connected to the multi-way joint, peristaltic pumps are connected to the middle parts of the first pipeline, the second pipeline and the third pipeline, one ends of the first pipeline and the second pipeline, which are far away from the multi-way joint, are connected with the supernatant recovery mechanism, one ends of the third pipeline, which are far away from the multi-way joint, are connected with the reaction tank, the lower end of the centrifugal barrel is connected with a connecting mechanism, the lower end of the connecting mechanism is connected with a driving motor, the joint of the centrifugal barrel and the multi-way joint is provided with a sealing ring, and the middle part inside the centrifugal barrel is provided with a centrifugal barrel inner pipe.
Description
Technical Field
The invention relates to the technical field of bioreactors, in particular to a centrifugal liquid exchange device.
Background
The manual or batch preparation of biopharmaceuticals, such as oncolytic viruses, UCART, using a bioreactor, and the adoption of a suitable cell culture process is key to the success of the culture process. In the medium replacement step, the medium needs to be replaced and supplemented according to the process requirements so as to ensure the pH value, the metabolite concentration, the nutrient substance concentration and the like, and ensure the rapid proliferation of cells or the output of products.
The existing common centrifugal liquid exchange and membrane filtration liquid exchange system has the defects of complex operation, high pollution risk and low speed due to the need of offline liquid discharge and liquid supplementing operation, so that a centrifugal liquid exchange device is needed to improve the problems.
Disclosure of Invention
The invention aims to provide a centrifugal liquid exchange device so as to solve the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a centrifugal liquid exchange device, includes centrifuge constructs, supernatant recovery mechanism and retort, centrifuge constructs by shell, centrifuge tube and base constitution, just the shell lower extreme is equipped with the base, the inside centrifuge tube that is equipped with of shell and base, centrifuge tube upper end is equipped with the multipath and connects, be connected with first pipeline, second pipeline and third pipeline on the multipath, the department all is connected with the peristaltic pump in the middle of first pipeline, second pipeline and the third pipeline, the one end that multipath was kept away from to first pipeline and second pipeline is connected with supernatant recovery mechanism, the one end that multipath was kept away from to the third pipeline is connected with the retort, centrifuge tube lower extreme is connected with coupling mechanism, the coupling mechanism lower extreme is connected with driving motor, centrifuge tube and multipath are connected department and are equipped with the sealing washer, centrifuge tube inside middle part is equipped with the centrifuge tube inner tube, centrifuge tube inside lower part is close to centrifuge tube inner tube department and is equipped with the bottom runner, the sealing washer adopts high-speed rotation sealing washer, connect through the sealing washer between centrifuge tube inner tube and the multipath, the bottom of centrifuge tube is the toper collecting surface structure, and be four sets of bottom equal distribution and bottom equal interval between the centrifuge tube structure.
Preferably, the driving motor is a high-precision servo motor.
Preferably, the connecting mechanism consists of a coupler and a bearing, and the centrifugal cylinder is connected with the driving motor through the coupler.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the centrifugal tube, the integrated centrifugal tube rotates through the arranged sealing ring, the cell fluid inside the centrifugal tube is layered under the action of centrifugal force, and the special high-speed rotating sealing ring is arranged between the inner tube of the centrifugal tube and the multipath connector, so that the sealing performance and the rotating performance in the centrifugal process are ensured.
2. According to the invention, through the centrifugal barrel, the bottom of the centrifugal barrel is of a conical collecting surface structure, and the special conical collecting surface design ensures that cells are centrifugally concentrated to the conical bottom in thickness, so that excessive cells are prevented from being taken away when supernatant is discharged.
3. According to the centrifugal liquid discharging and harvesting device, four groups of bottom flow channels are arranged and distributed in an annular equidistant mode, the bottom flow channels are communicated with the bottom of the inner tube of the centrifugal tube and the circumferential direction of the centrifugal tube, the bottom flow channels are matched with the bottom structure of the centrifugal tube, so that bottom residues are minimized when centrifugal liquid discharging and harvesting products are guaranteed, simultaneous operation of centrifugal rotation and liquid discharging is achieved, and accidental discharge of cells is avoided when static liquid is discharged.
4. According to the invention, through the multipath joint, the sample stock solution and the centrifuged waste liquid flow from the same central pipeline, and the multipath joint at the top layer realizes simultaneous operation of cell suspension, culture medium and discharged liquid, thereby greatly improving space and operation efficiency.
Drawings
FIG. 1 is a diagram of the overall structure of the present invention;
FIG. 2 is a split structure view of the centrifugal mechanism of the present invention;
FIG. 3 is a cross-sectional view of the centrifuge bowl according to the present invention;
FIG. 4 is a schematic diagram of a centrifugal mechanism according to the present invention.
In the figure: 1. a centrifugal mechanism; 2. a supernatant recovery mechanism; 3. a reaction tank; 4. a housing; 5. a centrifugal barrel; 6. a connecting mechanism; 7. a driving motor; 8. a base; 9. a multi-way joint; 10. a seal ring; 11. a centrifugal cylinder inner tube; 12. a bottom flow channel; 13. a peristaltic pump; 14. a first pipe; 15. a second pipe; 16. and a third pipeline.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making creative efforts based on the embodiments of the present invention are included in the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
a centrifugal liquid exchange device comprises a centrifugal mechanism 1, a supernatant recovery mechanism 2 and a reaction tank 3, wherein the centrifugal mechanism 1 consists of a shell 4, a centrifugal barrel 5 and a base 8, the lower end of the shell 4 is provided with the base 8, the centrifugal barrel 5 is arranged inside the shell 4 and the base 8, the bottom of the centrifugal barrel 5 is of a conical collecting surface structure, the upper end of the centrifugal barrel 5 is provided with a multi-way joint 9, a first pipeline 14, a second pipeline 15 and a third pipeline 16 are connected on the multi-way joint 9, peristaltic pumps 13 are respectively connected at the middle parts of the first pipeline 14, the second pipeline 15 and the third pipeline 16, one ends of the first pipeline 14 and the second pipeline 15, which are far away from the multi-way joint 9, are connected with the supernatant recovery mechanism 2, one ends of the third pipeline 16, which are far away from the multi-way joint 9, are connected with the reaction tank 3, the lower extreme of a centrifugal tube 5 is connected with a connecting mechanism 6, the lower extreme of the connecting mechanism 6 is connected with a driving motor 7, the driving motor 7 is a high-precision servo motor, the connecting mechanism 6 is composed of a coupler and a bearing, the centrifugal tube 5 is connected with the driving motor 7 through the coupler, a sealing ring 10 is arranged at the joint of the centrifugal tube 5 and a multi-way joint 9, a centrifugal tube inner tube 11 is arranged at the middle of the inside of the centrifugal tube 5, the sealing ring 10 is a high-speed rotary sealing ring, the centrifugal tube inner tube 11 is connected with the multi-way joint 9 through the sealing ring 10, a bottom runner 12 is arranged at the lower part of the inside of the centrifugal tube 5, close to the centrifugal tube inner tube 11, four groups of bottom runners 12 are distributed in an annular equidistant mode, and a communication structure is formed between the bottom runner 12 and the centrifugal tube inner tube 11.
The working flow of the invention is as follows: firstly, the driving motor 7 is a high-precision servo motor, the most suitable centrifugal rate can be matched according to the cell types, the optimal recovery efficiency and the optimal activity rate are realized, the connecting mechanism 6 consists of a coupler and a bearing, the centrifugal barrel 5 and the driving motor 7 are connected through the coupler, so that the direct impact of the load and vibration of the mechanism during high-speed centrifugation is avoided, the integrated centrifugal barrel 5 rotates through the arranged sealing ring 10, the layering of the internal cell liquid is realized under the action of centrifugal force, a special high-speed rotating sealing ring is arranged between the inner pipe 11 of the centrifugal barrel and the multipath connector 9, the sealing performance and the rotating performance in the centrifugation process are ensured, the bottom of the centrifugal barrel 5 is of a conical collecting surface structure through the arranged centrifugal barrel 5, the special conical collecting surface design ensures that the centrifugal thickness of the cells is gathered to the conical bottom, and the excessive cells are prevented from being taken away when the supernatant is discharged, compared with the traditional centrifugal bottle design, effectively separates liquid and cell areas, greatly reduces residual cell quantity, simultaneously reduces shearing force on cells during centrifugation, and through the arranged bottom flow channels 12, the bottom flow channels 12 are provided with four groups which are distributed in annular equidistant mode, and the bottom flow channels 12 and the inner tube 11 of the centrifugal tube are in a communication structure, the bottom flow channels 12 are communicated with the bottom of the inner tube 11 of the centrifugal tube and the circumferential direction of the centrifugal tube 5, and cooperate with the bottom structure of the centrifugal tube 5 to ensure that the residual quantity of the bottom is reduced to the minimum when centrifugal liquid is discharged and harvested products, realize simultaneous operation of centrifugal rotation and liquid discharge, avoid accidental discharge of cells when static liquid is discharged, realize simultaneous operation of cell suspension, culture medium and discharged liquid through the arranged multipath joint 9 of the top layer, flow the sample stock solution and the waste liquid from the same central pipeline, the whole structure is simple, the effect is good, and the stability and the practicability are higher.
Examples
Basic workflow: cell suspension in the reaction tank 3 continuously flows into the centrifugal cylinder 5 through the third pipeline 16, the centrifugal mechanism 1 achieves a stable working rotation speed according to a set acceleration curve, cells effectively cling to the conical surface of the cavity, the normal working rotation speed of the centrifugal mechanism 1 is kept, supernatant waste liquid is discharged into a supernatant waste liquid collecting bag of the supernatant recovery mechanism 2 through the second pipeline 15, the centrifugal mechanism 1 recovers a static state according to a set deceleration curve, a culture medium is supplemented into the centrifugal cylinder 5 through the first pipeline 14, the centrifugal cylinder 5 rotates clockwise and anticlockwise in a reciprocating mode, the cell re-suspension state is effectively recovered, the re-suspended cell suspension continuously flows back into the reaction tank 3 through the third pipeline 16, and the cells continue to culture and expand.
The production, centrifugation, product collection trinity's closed operation have been realized, the pollution risk when avoiding the different unit connection of traditional mode, simultaneously the work load in the significantly reduced connection process reaches the demand to the space, realize in the continuous perfusion process, "production is collected while" continuous production's demand, compare traditional centrifuge bottle design, effectively separate liquid, with the cell area, reduce the residual cell volume by a wide margin.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The utility model provides a centrifugal liquid device that trades, includes centrifugal mechanism (1), supernatant recovery mechanism (2) and retort (3), its characterized in that: the centrifugal mechanism (1) consists of a shell (4), a centrifugal tube (5) and a base (8), the lower end of the shell (4) is provided with the base (8), the centrifugal tube (5) is arranged inside the shell (4) and the base (8), the upper end of the centrifugal tube (5) is provided with a multi-way joint (9), a first pipeline (14), a second pipeline (15) and a third pipeline (16) are connected to the multi-way joint (9), peristaltic pumps (13) are respectively connected to the middle parts of the first pipeline (14), the second pipeline (15) and the third pipeline (16), one end of the first pipeline (14) and the second pipeline (15) away from the multi-way joint (9) is connected with a supernatant recovery mechanism (2), one end of the third pipeline (16) away from the multi-way joint (9) is connected with a reaction tank (3), the lower end of the centrifugal tube (5) is connected with a connecting mechanism (6), the lower end of the connecting mechanism (6) is connected with a driving motor (7), the middle parts of the first pipeline (14), the second pipeline (15) and the third pipeline (16) are respectively connected with peristaltic pumps (13), one end of the centrifugal tube (5) is provided with a centrifugal inner tube (10), one end of the centrifugal tube (5) is provided with a centrifugal sealing ring (11), one end of the centrifugal tube (11) is close to the inner tube (11), the centrifugal barrel is characterized in that the sealing ring (10) is a high-speed rotary sealing ring, the centrifugal barrel inner pipe (11) is connected with the multipath joint (9) through the sealing ring (10), the bottom of the centrifugal barrel (5) is of a conical collecting surface structure, four groups of bottom runners (12) are arranged and distributed in an annular equidistant mode, and a communicating structure is arranged between the bottom runners (12) and the centrifugal barrel inner pipe (11).
2. A centrifugal liquid exchange device according to claim 1, wherein: the driving motor (7) is a high-precision servo motor.
3. A centrifugal liquid exchange device according to claim 1, wherein: the connecting mechanism (6) consists of a coupler and a bearing, and the centrifugal cylinder (5) is connected with the driving motor (7) through the coupler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011403871.XA CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011403871.XA CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112570154A CN112570154A (en) | 2021-03-30 |
| CN112570154B true CN112570154B (en) | 2024-03-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011403871.XA Active CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112570154B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN103418503A (en) * | 2012-05-15 | 2013-12-04 | 美天施生物科技有限责任公司 | Centrifugation chamber with deflectors |
| CN105828948A (en) * | 2013-12-20 | 2016-08-03 | 3M创新有限公司 | Systems and methods for sample concentration and detection using a separation liquid |
| US10040077B1 (en) * | 2015-05-19 | 2018-08-07 | Pneumatic Scale Corporation | Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core |
| JP2020174557A (en) * | 2019-04-17 | 2020-10-29 | 株式会社日立製作所 | Cell culturing/refining apparatus and cell culturing/refining method |
| CN111992339A (en) * | 2020-07-28 | 2020-11-27 | 刘肖琳 | Centrifugal separation device, and method for separating and culturing mixed liquid |
| CN213996296U (en) * | 2020-12-03 | 2021-08-20 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal liquid changing device |
-
2021
- 2021-02-24 CN CN202011403871.XA patent/CN112570154B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN103418503A (en) * | 2012-05-15 | 2013-12-04 | 美天施生物科技有限责任公司 | Centrifugation chamber with deflectors |
| CN105828948A (en) * | 2013-12-20 | 2016-08-03 | 3M创新有限公司 | Systems and methods for sample concentration and detection using a separation liquid |
| US10040077B1 (en) * | 2015-05-19 | 2018-08-07 | Pneumatic Scale Corporation | Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core |
| JP2020174557A (en) * | 2019-04-17 | 2020-10-29 | 株式会社日立製作所 | Cell culturing/refining apparatus and cell culturing/refining method |
| CN111992339A (en) * | 2020-07-28 | 2020-11-27 | 刘肖琳 | Centrifugal separation device, and method for separating and culturing mixed liquid |
| CN213996296U (en) * | 2020-12-03 | 2021-08-20 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal liquid changing device |
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| Publication number | Publication date |
|---|---|
| CN112570154A (en) | 2021-03-30 |
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