CN112570154A - Centrifugal liquid changing device - Google Patents
Centrifugal liquid changing device Download PDFInfo
- Publication number
- CN112570154A CN112570154A CN202011403871.XA CN202011403871A CN112570154A CN 112570154 A CN112570154 A CN 112570154A CN 202011403871 A CN202011403871 A CN 202011403871A CN 112570154 A CN112570154 A CN 112570154A
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- Prior art keywords
- centrifugal
- pipeline
- centrifugal cylinder
- way joint
- cylinder
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- 239000007788 liquid Substances 0.000 title claims abstract description 30
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 10
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims description 18
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- 238000004064 recycling Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 230000003068 static effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000309459 oncolytic virus Species 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
- B04B7/08—Rotary bowls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/02—Electric motor drives
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/16—Sealings between relatively-moving surfaces
Landscapes
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Centrifugal Separators (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of bioreactors, in particular to a centrifugal liquid changing device which comprises a centrifugal mechanism, a supernatant liquid recycling mechanism and a reaction tank, wherein the centrifugal mechanism consists of a shell, a centrifugal cylinder and a base, the lower end of the shell is provided with the base, the centrifugal cylinder is arranged inside the shell and the base, the upper end of the centrifugal cylinder is provided with a multi-way joint, the multi-way joint is connected with a first pipeline, a second pipeline and a third pipeline, peristaltic pumps are respectively connected among the first pipeline, the second pipeline and the third pipeline, one ends of the first pipeline and the second pipeline, which are far away from the multi-way joint, are connected with the supernatant liquid recycling mechanism, one end of the third pipeline, which is far away from the multi-way joint, is connected with the reaction tank, the lower end of the centrifugal cylinder is connected with a connecting mechanism, the lower end of the connecting mechanism is connected with a driving motor, the middle of the inner part of the centrifugal cylinder is provided with the inner pipe of the centrifugal cylinder, so that the integral effect is good.
Description
Technical Field
The invention relates to the technical field of bioreactors, in particular to a centrifugal liquid changing device.
Background
The biological medicines such as oncolytic virus and UCART are prepared manually or in batches by using a bioreactor, and the key for the success of the culture process is the adoption of a proper cell culture process. In the medium replacement step, the medium needs to be replaced and supplemented according to the process requirements to ensure the pH value, the metabolite concentration, the nutrient concentration and the like, and to ensure the rapid proliferation of cells or the product output.
The conventional centrifugal liquid changing and membrane filtering liquid changing system has the defects of off-line liquid discharging and supplementing operations, complex operation, high pollution risk and low speed, so that a centrifugal liquid changing device is needed to improve the problems.
Disclosure of Invention
The present invention is directed to a centrifugal liquid-changing device to solve the above problems.
In order to achieve the purpose, the invention provides the following technical scheme:
a centrifugal liquid changing device comprises a centrifugal mechanism, a supernatant recovery mechanism and a reaction tank, wherein the centrifugal mechanism comprises a shell, a centrifugal cylinder and a base, the lower end of the shell is provided with the base, centrifugal cylinders are arranged in the shell and the base, the upper end of the centrifugal cylinder is provided with a multi-way joint, a first pipeline, a second pipeline and a third pipeline are connected onto the multi-way joint, peristaltic pumps are connected among the first pipeline, the second pipeline and the third pipeline, one ends of the first pipeline and the second pipeline, which are far away from the multi-way joint, are connected with the supernatant recovery mechanism, one end of the third pipeline, which is far away from the multi-way joint, is connected with the reaction tank, the lower end of the centrifugal cylinder is connected with a connecting mechanism, the lower end of the connecting mechanism is connected with a driving motor, a sealing ring is arranged at the joint of the centrifugal cylinder and the multi-way joint, and a bottom flow passage is arranged at the lower part of the interior of the centrifugal cylinder, which is close to the inner pipe of the centrifugal cylinder.
Preferably, the driving motor is a high-precision servo motor.
Preferably, the connecting mechanism is composed of a coupler and a bearing, and the centrifugal cylinder is connected with the driving motor through the coupler.
Preferably, the sealing ring is a high-speed rotating sealing ring, and the inner pipe of the centrifugal cylinder is connected with the multi-way joint through the sealing ring.
Preferably, the bottom of the centrifugal cylinder is of a conical collecting surface structure.
Preferably, the bottom runners are provided with four groups and distributed in an annular shape at equal intervals, and a communicating structure is formed between the bottom runners and the inner tube of the centrifugal cylinder.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the integrated centrifugal cylinder rotates through the arranged sealing ring, the cellular fluid in the integrated centrifugal cylinder is layered under the action of centrifugal force, and the special high-speed rotating sealing ring is arranged between the inner pipe of the centrifugal cylinder and the multi-way connector, so that the sealing performance and the rotating performance in the centrifugal process are ensured.
2. According to the invention, through the arranged centrifugal cylinder, the bottom of the centrifugal cylinder is of a conical collection surface structure, and the special conical collection surface design ensures that cells are centrifugally thickly gathered to the conical bottom, so that excessive cells are prevented from being carried away when supernatant is discharged.
3. According to the centrifugal liquid discharging device, four groups of bottom runners are arranged and distributed at equal intervals in an annular mode, the bottom runners are in a communicating structure with the inner tube of the centrifugal cylinder, the bottom runners are communicated with the bottom of the inner tube of the centrifugal cylinder and the circumferential direction of the centrifugal cylinder and matched with the bottom structure of the centrifugal cylinder, so that the bottom residue is reduced to the minimum when liquid is discharged and harvested in a centrifugal mode, the simultaneous work of centrifugal rotation and liquid discharge is realized, and the accidental discharge of cells when static liquid is discharged is avoided.
4. According to the invention, through the arranged multi-way joint, the sample stock solution and the centrifuged waste liquid flow from the same central pipeline, and the multi-way joint on the top layer realizes the simultaneous operation of cell suspension, culture medium and discharged liquid, thereby greatly improving the space and the operation efficiency.
Drawings
FIG. 1 is an overall structural view of the present invention;
FIG. 2 is a split structure view of the centrifugal mechanism of the present invention;
FIG. 3 is a cross-sectional structural view of a centrifuge bowl according to the present invention;
FIG. 4 is a diagram of a centrifugal mechanism according to the present invention.
In the figure: 1. a centrifugal mechanism; 2. a supernatant recovery mechanism; 3. a reaction tank; 4. a housing; 5. a centrifugal cylinder; 6. a connecting mechanism; 7. a drive motor; 8. a base; 9. a multi-way joint; 10. a seal ring; 11. an inner tube of the centrifugal cylinder; 12. a bottom runner; 13. a peristaltic pump; 14. a first conduit; 15. a second conduit; 16. a third conduit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
a centrifugal liquid changing device comprises a centrifugal mechanism 1, a supernatant recovery mechanism 2 and a reaction tank 3, wherein the centrifugal mechanism 1 comprises a shell 4, a centrifugal cylinder 5 and a base 8, the lower end of the shell 4 is provided with the base 8, the centrifugal cylinder 5 is arranged inside the shell 4 and the base 8, the bottom of the centrifugal cylinder 5 is of a conical collecting surface structure, the upper end of the centrifugal cylinder 5 is provided with a multi-way joint 9, the multi-way joint 9 is connected with a first pipeline 14, a second pipeline 15 and a third pipeline 16, peristaltic pumps 13 are respectively connected at the middles of the first pipeline 14, the second pipeline 15 and the third pipeline 16, one ends of the first pipeline 14 and the second pipeline 15, which are far away from the multi-way joint 9, are connected with the supernatant recovery mechanism 2, one end of the third pipeline 16, which is far away from the multi-way joint 9, is connected with the reaction tank 3, the lower end of the centrifugal cylinder 5 is connected with a connecting mechanism 6, the lower, the connecting mechanism 6 is composed of a coupler and a bearing, the centrifugal cylinder 5 is connected with the driving motor 7 through the coupler, a sealing ring 10 is arranged at the joint of the centrifugal cylinder 5 and the multi-way joint 9, a centrifugal cylinder inner pipe 11 is arranged in the middle of the inside of the centrifugal cylinder 5, the sealing ring 10 is a high-speed rotating sealing ring, the centrifugal cylinder inner pipe 11 is connected with the multi-way joint 9 through the sealing ring 10, bottom runners 12 are arranged at the lower part of the inside of the centrifugal cylinder 5, close to the centrifugal cylinder inner pipe 11, four groups of the bottom runners 12 are arranged and distributed in an annular equidistant mode, and a communicating structure is formed between the bottom runners 12 and the centrifugal.
The working process of the invention is as follows: firstly, the driving motor 7 is a high-precision servo motor, the most appropriate centrifugal speed can be matched according to the cell types, the optimal recovery efficiency and the optimal survival rate are realized, the connecting mechanism 6 consists of a coupler and a bearing, the centrifugal cylinder 5 is connected with the driving motor 7 through the coupler so as to prevent the load and the vibration of the mechanism from directly impacting the motor during high-speed centrifugation, the integrated centrifugal cylinder 5 rotates through the arranged sealing ring 10, the inner cell liquid is layered under the action of centrifugal force, a special high-speed rotating sealing ring is arranged between the centrifugal cylinder inner tube 11 and the multi-way joint 9 so as to ensure the sealing performance and the rotating performance during centrifugation, the bottom of the centrifugal cylinder 5 is of a conical collecting surface structure through the arranged centrifugal cylinder 5, the special conical collecting surface design is adopted to ensure that the cells are centrifugally thickly accumulated to the conical bottom, and the cells are prevented from being carried away when supernatant is discharged, compared with the traditional centrifugal bottle, liquid and cell areas are effectively separated, the amount of residual cells is greatly reduced, meanwhile, the shearing force to the cells during centrifugation is reduced, four groups of bottom runners 12 are arranged on the bottom runners 12 and are distributed at equal intervals in an annular mode, a communicating structure is formed between the bottom runners 12 and a centrifuge tube inner tube 11, the bottom runners 12 are communicated with the bottom of the centrifuge tube inner tube 11 and the circumferential direction of a centrifuge tube 5 and are matched with the bottom structure of the centrifuge tube 5, so that the bottom residue is reduced to the minimum during centrifugation liquid drainage and product harvesting, the simultaneous work of centrifugation rotation and liquid drainage is realized, the accidental discharge of the cells during static liquid drainage is avoided, sample stock solution and centrifuged waste liquid flow from the same central pipeline through the arranged multi-way joint 9, the multi-way joint 9 on the top layer realizes the simultaneous operation of cell suspension, culture medium and discharged liquid, and the space and operation efficiency are greatly improved, the whole structure is simple, the effect is good, and the stability and the practicability are higher.
Examples
Basic work flow: cell suspension in the reaction tank 3 continuously perfuses into the centrifugal cylinder 5 through the third pipeline 16, the centrifugal mechanism 1 reaches a stable working speed according to a set acceleration curve, cells effectively adhere to the wall on the conical surface of the cavity, the normal working speed of the centrifugal mechanism 1 is kept, supernatant waste liquid is discharged into a supernatant waste liquid collecting bag of the supernatant liquid recycling mechanism 2 through the second pipeline 15, the centrifugal mechanism 1 recovers a static state according to a set deceleration curve, a culture medium is supplemented into the centrifugal cylinder 5 through the first pipeline 14, the centrifugal cylinder 5 rotates clockwise and anticlockwise in a reciprocating mode, the cell re-suspension state is effectively recovered, the re-suspended cell suspension is continuously perfused back into the reaction tank 3 through the third pipeline 16, and the cells continue to be cultured and expanded.
The sealed operation of production, centrifugation, trinity is collected to the result has been realized, pollution risk when avoiding the different unit connection of traditional mode, and work load in the connection that significantly reduces simultaneously reaches the demand to the space, realizes the in-process of continuous perfusion, "while production, the demand of collecting" continuous production simultaneously compares traditional centrifuge bottle design, effectively separates liquid, with the cell region, reduces substantially and remains the cell volume.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a centrifugation liquid changing device, includes that centrifugation mechanism (1), supernatant retrieve mechanism (2) and retort (3), its characterized in that: the centrifugal mechanism (1) is composed of a shell (4), a centrifugal cylinder (5) and a base (8), the base (8) is arranged at the lower end of the shell (4), the centrifugal cylinder (5) is arranged inside the shell (4) and the base (8), a multi-way joint (9) is arranged at the upper end of the centrifugal cylinder (5), a first pipeline (14), a second pipeline (15) and a third pipeline (16) are connected onto the multi-way joint (9), peristaltic pumps (13) are connected to the middle positions of the first pipeline (14), the second pipeline (15) and the third pipeline (16), one ends of the first pipeline (14) and the second pipeline (15) far away from the multi-way joint (9) are connected with a supernatant recovery mechanism (2), one end of the third pipeline (16) far away from the multi-way joint (9) is connected with a reaction tank (3), and a connecting mechanism (6) is connected to the lower end of the centrifugal cylinder (5), the centrifugal pump is characterized in that the lower end of the connecting mechanism (6) is connected with a driving motor (7), a sealing ring (10) is arranged at the joint of the centrifugal cylinder (5) and the multi-way connector (9), a centrifugal cylinder inner tube (11) is arranged in the middle of the inside of the centrifugal cylinder (5), and a bottom flow channel (12) is arranged at the position, close to the centrifugal cylinder inner tube (11), of the lower part of the inside of the centrifugal cylinder (5).
2. A centrifugal fluid exchange device according to claim 1, wherein: the driving motor (7) is a high-precision servo motor.
3. A centrifugal fluid exchange device according to claim 1, wherein: the connecting mechanism (6) is composed of a coupler and a bearing, and the centrifugal cylinder (5) is connected with the driving motor (7) through the coupler.
4. A centrifugal fluid exchange device according to claim 1, wherein: the sealing ring (10) adopts a high-speed rotating sealing ring, and the centrifugal cylinder inner tube (11) is connected with the multi-way joint (9) through the sealing ring (10).
5. A centrifugal fluid exchange device according to claim 1, wherein: the bottom of the centrifugal cylinder (5) is of a conical collecting surface structure.
6. A centrifugal fluid exchange device according to claim 1, wherein: the bottom runners (12) are arranged in four groups and distributed in an annular shape at equal intervals, and a communicating structure is formed between the bottom runners (12) and the inner tube (11) of the centrifugal cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011403871.XA CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011403871.XA CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112570154A true CN112570154A (en) | 2021-03-30 |
| CN112570154B CN112570154B (en) | 2024-03-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011403871.XA Active CN112570154B (en) | 2021-02-24 | 2021-02-24 | Centrifugal liquid changing device |
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| Country | Link |
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| CN (1) | CN112570154B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN103418503A (en) * | 2012-05-15 | 2013-12-04 | 美天施生物科技有限责任公司 | Centrifugation chamber with deflectors |
| CN105828948A (en) * | 2013-12-20 | 2016-08-03 | 3M创新有限公司 | Systems and methods for sample concentration and detection using a separation liquid |
| US10040077B1 (en) * | 2015-05-19 | 2018-08-07 | Pneumatic Scale Corporation | Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core |
| JP2020174557A (en) * | 2019-04-17 | 2020-10-29 | 株式会社日立製作所 | Cell culturing/refining apparatus and cell culturing/refining method |
| CN111992339A (en) * | 2020-07-28 | 2020-11-27 | 刘肖琳 | Centrifugal separation device, and method for separating and culturing mixed liquid |
| CN213996296U (en) * | 2020-12-03 | 2021-08-20 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal liquid changing device |
-
2021
- 2021-02-24 CN CN202011403871.XA patent/CN112570154B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN103418503A (en) * | 2012-05-15 | 2013-12-04 | 美天施生物科技有限责任公司 | Centrifugation chamber with deflectors |
| CN105828948A (en) * | 2013-12-20 | 2016-08-03 | 3M创新有限公司 | Systems and methods for sample concentration and detection using a separation liquid |
| US10040077B1 (en) * | 2015-05-19 | 2018-08-07 | Pneumatic Scale Corporation | Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core |
| JP2020174557A (en) * | 2019-04-17 | 2020-10-29 | 株式会社日立製作所 | Cell culturing/refining apparatus and cell culturing/refining method |
| CN111992339A (en) * | 2020-07-28 | 2020-11-27 | 刘肖琳 | Centrifugal separation device, and method for separating and culturing mixed liquid |
| CN213996296U (en) * | 2020-12-03 | 2021-08-20 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal liquid changing device |
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|---|---|
| CN112570154B (en) | 2024-03-19 |
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