CN112567080A - 制备噬菌体文库的方法 - Google Patents

制备噬菌体文库的方法 Download PDF

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CN112567080A
CN112567080A CN202080004535.XA CN202080004535A CN112567080A CN 112567080 A CN112567080 A CN 112567080A CN 202080004535 A CN202080004535 A CN 202080004535A CN 112567080 A CN112567080 A CN 112567080A
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vector
heavy chain
light chain
library
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周辰
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Taki Zhan Shanghai Biotechnology Co ltd
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Taki Zhan Shanghai Biotechnology Co ltd
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
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    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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Abstract

提供了一种产生展示抗体或抗体片段的噬菌体文库的方法，包括提供包含LC的第一多核苷酸、包含连接子的第二多核苷酸和包含HC的第三多核苷酸，将第一、第二和第三多核苷酸分别导入第一、第二和第三细菌中获得轻链组件细菌文库、连接子组件细菌和重链组件细菌文库，从文库获得轻链组件质粒、连接子组件质粒和重链组件质粒，从中获得经释放的LC、经释放的连接子和经释放的HC,与经释放的展示载体片段连接形成展示用连接产物，导入第四细菌中获得展示细菌文库，使用所述展示细菌文库制备展示所述抗体或抗体片段的噬菌体文库。还提供了根据该方法产生的噬菌体文库。

Description

PCT国内申请，说明书已公开。

Claims (87)

  1. PCT国内申请，权利要求书已公开。
CN202080004535.XA 2019-04-22 2020-04-20 制备噬菌体文库的方法 Pending CN112567080A (zh)

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Application Number Priority Date Filing Date Title
CN2019103277396 2019-04-22
CN201910327739 2019-04-22
PCT/CN2020/085706 WO2020216191A1 (zh) 2019-04-22 2020-04-20 制备噬菌体文库的方法

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CN112567080A true CN112567080A (zh) 2021-03-26

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US (1) US20220228138A1 (zh)
EP (1) EP3960916A4 (zh)
JP (1) JP2022532699A (zh)
KR (1) KR20210153674A (zh)
CN (1) CN112567080A (zh)
BR (1) BR112021021261A2 (zh)
MX (1) MX2021012914A (zh)
WO (1) WO2020216191A1 (zh)

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MX2023011638A (es) 2021-03-31 2023-10-13 Ddbio Co Ltd Shang Hai Vectores y metodo para el cribado de proteinas de union a antigeno funcionales.
CA3241327A1 (en) * 2021-12-23 2023-06-29 Generation Bio Co. Scalable and high-purity cell-free synthesis of closed-ended dna vectors

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101210241A (zh) * 2006-12-27 2008-07-02 陕西超英生物科技有限公司 一种噬菌体基因工程抗体库基因组装的方法
CN101720368A (zh) * 2007-03-09 2010-06-02 中国抗体制药有限公司 储备多样性最大化的功能性人化抗体文库之构建及应用
CN102732974A (zh) * 2012-07-16 2012-10-17 百泰生物药业有限公司 噬菌体抗体库的构建方法及应用此库筛选到的抗cd6抗体

Family Cites Families (5)

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ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US6261558B1 (en) * 1993-10-19 2001-07-17 The Scripps Research Institute Synthetic human neutralizing monoclonal antibodies to human immunodeficiency virus
ATE434040T1 (de) * 2001-10-01 2009-07-15 Dyax Corp Mehrkettige eukaryontische display-vektoren und deren verwendungen
EP3475700A4 (en) * 2016-06-26 2020-03-04 Gennova Biopharmaceuticals Limited PHAGE ANTIBODY PRESENTATION BANK
US10415033B2 (en) * 2017-07-31 2019-09-17 Academia Sinica Phage-displayed single-chain variable fragment libraries and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101210241A (zh) * 2006-12-27 2008-07-02 陕西超英生物科技有限公司 一种噬菌体基因工程抗体库基因组装的方法
CN101720368A (zh) * 2007-03-09 2010-06-02 中国抗体制药有限公司 储备多样性最大化的功能性人化抗体文库之构建及应用
CN102732974A (zh) * 2012-07-16 2012-10-17 百泰生物药业有限公司 噬菌体抗体库的构建方法及应用此库筛选到的抗cd6抗体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XIAODONG CEN 等: "Construction of a large phage display antibody library by in vitro package and in vivo recombination", 《APPLIED MICROBIOLOGY AND BIOTECHNOLOGY》 *

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MX2021012914A (es) 2022-01-18
EP3960916A4 (en) 2023-01-18
EP3960916A1 (en) 2022-03-02
KR20210153674A (ko) 2021-12-17
JP2022532699A (ja) 2022-07-19
WO2020216191A1 (zh) 2020-10-29
US20220228138A1 (en) 2022-07-21
BR112021021261A2 (pt) 2021-12-21

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