MX2021012914A - Método para preparar genotecas de fagos. - Google Patents

Método para preparar genotecas de fagos.

Info

Publication number
MX2021012914A
MX2021012914A MX2021012914A MX2021012914A MX2021012914A MX 2021012914 A MX2021012914 A MX 2021012914A MX 2021012914 A MX2021012914 A MX 2021012914A MX 2021012914 A MX2021012914 A MX 2021012914A MX 2021012914 A MX2021012914 A MX 2021012914A
Authority
MX
Mexico
Prior art keywords
library
released
connexon
display
phage library
Prior art date
Application number
MX2021012914A
Other languages
English (en)
Inventor
Chen Zhou
Original Assignee
Ddbio Co Ltd Shang Hai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ddbio Co Ltd Shang Hai filed Critical Ddbio Co Ltd Shang Hai
Publication of MX2021012914A publication Critical patent/MX2021012914A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/243—Colony Stimulating Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Bioinformatics & Computational Biology (AREA)

Abstract

Se proporciona un método para producir una genoteca de fagos que muestra anticuerpos o fragmentos de anticuerpos, que comprende proporcionar un primer polinucleótido que contiene LC, un segundo polinucleótido que contiene conexona y un tercer polinucleótido que contiene HC, introduciendo respectivamente el primer, segundo y tercer polinucleótidos en la primera, segunda y tercera bacterias para obtener una genoteca bacteriana del componente de cadena ligera, una genoteca bacteriana del componente de conexona y una genoteca bacteriana del componente de cadena pesada, obteniendo un plásmido del componente de cadena ligera, un plásmido de componente de conexona y un plásmido del componente de cadena pesada de las genotecas, obteniendo la LC liberada, conexona liberada y HC liberada de los plásmidos, conectando los segmentos del vector de visualización liberados para formar un producto de conexión para la visualización, introduciendo una tercera bacteria para obtener una genoteca bacteriana de visualización y utilizando la genoteca bacteriana de visualización para preparar la genoteca de fagos para mostrar los anticuerpos o fragmentos de anticuerpos; también se proporciona una genoteca de fagos producida de acuerdo con el método.
MX2021012914A 2019-04-22 2020-04-20 Método para preparar genotecas de fagos. MX2021012914A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910327739 2019-04-22
PCT/CN2020/085706 WO2020216191A1 (zh) 2019-04-22 2020-04-20 制备噬菌体文库的方法

Publications (1)

Publication Number Publication Date
MX2021012914A true MX2021012914A (es) 2022-01-18

Family

ID=72941349

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021012914A MX2021012914A (es) 2019-04-22 2020-04-20 Método para preparar genotecas de fagos.

Country Status (8)

Country Link
US (1) US20220228138A1 (es)
EP (1) EP3960916A4 (es)
JP (1) JP2022532699A (es)
KR (1) KR20210153674A (es)
CN (1) CN112567080A (es)
BR (1) BR112021021261A2 (es)
MX (1) MX2021012914A (es)
WO (1) WO2020216191A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112023019896A2 (pt) 2021-03-31 2023-11-07 Ddbio Co Ltd Shang Hai Vetores e método para triagem de proteína funcional de ligação ao antígeno
WO2023122303A2 (en) * 2021-12-23 2023-06-29 Generation Bio Co. Scalable and high-purity cell-free synthesis of closed-ended dna vectors

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
ATE405679T1 (de) * 1993-10-19 2008-09-15 Scripps Research Inst Synthetische humane neutralisierende monoklonale antikörper gegen hiv
AU2002330162B2 (en) * 2001-10-01 2008-08-07 Takeda Pharmaceutical Company Limited Multi-chain eukaryotic display vectors and uses thereof
CN101210241A (zh) * 2006-12-27 2008-07-02 陕西超英生物科技有限公司 一种噬菌体基因工程抗体库基因组装的方法
CN101720368A (zh) * 2007-03-09 2010-06-02 中国抗体制药有限公司 储备多样性最大化的功能性人化抗体文库之构建及应用
CN102732974B (zh) * 2012-07-16 2014-01-15 百泰生物药业有限公司 噬菌体抗体库的构建方法及应用此库筛选到的抗cd6抗体
AU2017289987B2 (en) * 2016-06-26 2022-06-23 Gennova Biopharmaceuticals Limited, Antibody phage display library
US10415033B2 (en) * 2017-07-31 2019-09-17 Academia Sinica Phage-displayed single-chain variable fragment libraries and uses thereof

Also Published As

Publication number Publication date
US20220228138A1 (en) 2022-07-21
CN112567080A (zh) 2021-03-26
WO2020216191A1 (zh) 2020-10-29
EP3960916A4 (en) 2023-01-18
KR20210153674A (ko) 2021-12-17
BR112021021261A2 (pt) 2021-12-21
JP2022532699A (ja) 2022-07-19
EP3960916A1 (en) 2022-03-02

Similar Documents

Publication Publication Date Title
MX2021012914A (es) Método para preparar genotecas de fagos.
AR105654A1 (es) Anticuerpos pd-l1 (ligando 1 de muerte celular programada)
CR20200171A (es) Contorsbodies 2 + 1 biespecíficos
TN2017000552A1 (en) Antibody constructs for msln and cd3.
EP3826612A4 (en) NOVEL MONOCLONAL ANTIBODIES SPECIFIC TO CLDN 18.2 AND THEIR METHODS OF USE
MY186296A (en) Novel promoter and use thereof
MX2022014463A (es) Construcciones de union a antigeno para moleculas diana.
NZ728874A (en) Anti-pd-1 monoclonal antibody and obtaining method therefor
PH12019501089A1 (en) Anti-pd-1/anti-her2 natural antibody structural heterodimeric bispecific antibody and method of preparing the same
MX2009010781A (es) Productos de proteina estructurados y de color.
WO2010033229A3 (en) Methods and vectors for display of 2g12 -derived domain exchanged antibodies
GB0506059D0 (en) Methods and apparatus for switching between data streams
EP4049676A4 (en) METHOD OF PRODUCING MONOVALENT CCAP PRODUCT
MY182444A (en) Igf-1r antibody and its use as addressing vehicle for the treatment of cancer
CO2020002156A2 (es) Produccion a gran escala de productos líquidos y solidos de trichoderma
MX2019008241A (es) Un enlazador polipeptídico para preparar anticuerpos multispecíficos.
TW200617011A (en) Process for preparing quinoline compounds and products obtained therefrom
MX2022003804A (es) Metodo de fabricacion.
MY197735A (en) Pharmaceutical combinations comprising an anti-ly75 antibody
WO2014176327A3 (en) Selection of fab fragments using ribosomal display technology
EP3641415A4 (en) SYNCHRONIZATION METHOD FOR SYNCHRONIZATION SIGNAL BLOCK, AND ASSOCIATED PRODUCT
EP3752535A4 (en) MONOCLONAL ANTIBODY PRODUCTION PROCESS HU14.18K322A
EP3754026A4 (en) METHOD FOR MANUFACTURING A FERMENTATION PRODUCT
WO2018220434A3 (de) Erdloskulturbehälter-formende platten
MX2023000547A (es) Anticuerpos anti-betacelulina, fragmentos de los mismos, y moleculas de union multiespecificas.