CN112566644A - 牙源干细胞及其用途 - Google Patents
牙源干细胞及其用途 Download PDFInfo
- Publication number
- CN112566644A CN112566644A CN201880093954.8A CN201880093954A CN112566644A CN 112566644 A CN112566644 A CN 112566644A CN 201880093954 A CN201880093954 A CN 201880093954A CN 112566644 A CN112566644 A CN 112566644A
- Authority
- CN
- China
- Prior art keywords
- stem cells
- dental
- dental pulp
- psoriasis
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 120
- 210000005258 dental pulp stem cell Anatomy 0.000 claims abstract description 81
- 201000004681 Psoriasis Diseases 0.000 claims abstract description 47
- 201000010099 disease Diseases 0.000 claims abstract description 25
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 25
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims abstract description 6
- 238000011282 treatment Methods 0.000 claims description 60
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 claims description 35
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 claims description 31
- 210000003954 umbilical cord Anatomy 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 13
- 230000002757 inflammatory effect Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 210000004489 deciduous teeth Anatomy 0.000 claims description 5
- 210000002379 periodontal ligament Anatomy 0.000 claims description 5
- 239000003937 drug carrier Substances 0.000 claims description 4
- 102000057308 human HGF Human genes 0.000 claims description 4
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- 230000001603 reducing effect Effects 0.000 claims description 4
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 22
- 101150022655 HGF gene Proteins 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 241000699670 Mus sp. Species 0.000 description 30
- 229960002751 imiquimod Drugs 0.000 description 27
- DOUYETYNHWVLEO-UHFFFAOYSA-N imiquimod Chemical compound C1=CC=CC2=C3N(CC(C)C)C=NC3=C(N)N=C21 DOUYETYNHWVLEO-UHFFFAOYSA-N 0.000 description 27
- 210000004027 cell Anatomy 0.000 description 24
- 238000002347 injection Methods 0.000 description 20
- 239000007924 injection Substances 0.000 description 20
- 206010040882 skin lesion Diseases 0.000 description 20
- 231100000444 skin lesion Toxicity 0.000 description 20
- 239000003814 drug Substances 0.000 description 16
- 230000001225 therapeutic effect Effects 0.000 description 14
- 102000013691 Interleukin-17 Human genes 0.000 description 11
- 108050003558 Interleukin-17 Proteins 0.000 description 11
- 229940079593 drug Drugs 0.000 description 11
- 230000008595 infiltration Effects 0.000 description 11
- 238000001764 infiltration Methods 0.000 description 11
- 210000002966 serum Anatomy 0.000 description 11
- 210000003462 vein Anatomy 0.000 description 11
- 210000004969 inflammatory cell Anatomy 0.000 description 10
- 238000013309 psoriasis mouse model Methods 0.000 description 10
- 210000000952 spleen Anatomy 0.000 description 10
- 108090001005 Interleukin-6 Proteins 0.000 description 9
- 230000006698 induction Effects 0.000 description 9
- 210000003074 dental pulp Anatomy 0.000 description 8
- 230000001575 pathological effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 208000024891 symptom Diseases 0.000 description 8
- 206010040867 Skin hypertrophy Diseases 0.000 description 7
- 210000002615 epidermis Anatomy 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 238000012258 culturing Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000002560 therapeutic procedure Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 5
- 210000003491 skin Anatomy 0.000 description 5
- 241000701161 unidentified adenovirus Species 0.000 description 5
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- 208000001126 Keratosis Diseases 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 4
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 4
- LWQQLNNNIPYSNX-UROSTWAQSA-N calcipotriol Chemical compound C1([C@H](O)/C=C/[C@@H](C)[C@@H]2[C@]3(CCCC(/[C@@H]3CC2)=C\C=C\2C([C@@H](O)C[C@H](O)C/2)=C)C)CC1 LWQQLNNNIPYSNX-UROSTWAQSA-N 0.000 description 4
- 229960002882 calcipotriol Drugs 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 238000009168 stem cell therapy Methods 0.000 description 4
- 238000009580 stem-cell therapy Methods 0.000 description 4
- 210000000434 stratum corneum Anatomy 0.000 description 4
- 238000010254 subcutaneous injection Methods 0.000 description 4
- 239000007929 subcutaneous injection Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 206010015150 Erythema Diseases 0.000 description 3
- 102000006354 HLA-DR Antigens Human genes 0.000 description 3
- 108010058597 HLA-DR Antigens Proteins 0.000 description 3
- 230000009815 adipogenic differentiation Effects 0.000 description 3
- 230000002293 adipogenic effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 230000002500 effect on skin Effects 0.000 description 3
- 206010020718 hyperplasia Diseases 0.000 description 3
- 230000003780 keratinization Effects 0.000 description 3
- 210000002510 keratinocyte Anatomy 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000011164 ossification Effects 0.000 description 3
- 230000009818 osteogenic differentiation Effects 0.000 description 3
- 230000001185 psoriatic effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 102100022464 5'-nucleotidase Human genes 0.000 description 2
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 241000702421 Dependoparvovirus Species 0.000 description 2
- 102100037241 Endoglin Human genes 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 description 2
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 2
- 101000881679 Homo sapiens Endoglin Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101001046686 Homo sapiens Integrin alpha-M Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 2
- 102100022338 Integrin alpha-M Human genes 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000011759 adipose tissue development Effects 0.000 description 2
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 238000001815 biotherapy Methods 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 108010007093 dispase Proteins 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010253 intravenous injection Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000003340 mental effect Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 239000002674 ointment Substances 0.000 description 2
- 230000002188 osteogenic effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 206010037844 rash Diseases 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 230000037380 skin damage Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 229960001727 tretinoin Drugs 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WHTVZRBIWZFKQO-AWEZNQCLSA-N (S)-chloroquine Chemical compound ClC1=CC=C2C(N[C@@H](C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-AWEZNQCLSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 description 1
- 229930105110 Cyclosporin A Natural products 0.000 description 1
- 108010036949 Cyclosporine Proteins 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 206010015866 Extravasation Diseases 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 1
- 208000005775 Parakeratosis Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 206010061372 Streptococcal infection Diseases 0.000 description 1
- 206010046306 Upper respiratory tract infection Diseases 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000001467 acupuncture Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 206010001093 acute tonsillitis Diseases 0.000 description 1
- 230000001800 adrenalinergic effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960003677 chloroquine Drugs 0.000 description 1
- WHTVZRBIWZFKQO-UHFFFAOYSA-N chloroquine Natural products ClC1=CC=C2C(NC(C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-UHFFFAOYSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229960001265 ciclosporin Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 229930182912 cyclosporin Natural products 0.000 description 1
- 230000002996 emotional effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002327 eosinophilic effect Effects 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 230000036251 extravasation Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 239000003410 keratolytic agent Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 238000011866 long-term treatment Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003821 menstrual periods Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229940126701 oral medication Drugs 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- 238000001126 phototherapy Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 208000020029 respiratory tract infectious disease Diseases 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002660 stem cell treatment Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940037128 systemic glucocorticoids Drugs 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 210000004357 third molar Anatomy 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
- 239000003860 topical agent Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 210000001644 umbilical artery Anatomy 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 150000003704 vitamin D3 derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/06—Antipsoriatics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
干细胞和基因修饰的干细胞的用途。特别是牙源干细胞和基因修饰的牙源干细胞在制备用于治疗银屑病及相关疾病的产品中的用途。包含牙源干细胞和/或基因修饰的牙源干细胞的组合物或试剂盒。HGF基因修饰的牙髓干细胞治疗银屑病的作用优于牙髓干细胞,HGF和牙源干细胞可以协同用于银屑病相关疾病的治疗。
Description
PCT国内申请,说明书已公开。
Claims (10)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2018107817539 | 2018-07-17 | ||
CN201810781753 | 2018-07-17 | ||
PCT/CN2018/116834 WO2020015264A1 (zh) | 2018-07-17 | 2018-11-22 | 牙源干细胞及其用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112566644A true CN112566644A (zh) | 2021-03-26 |
Family
ID=69165015
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201880093954.8A Pending CN112566644A (zh) | 2018-07-17 | 2018-11-22 | 牙源干细胞及其用途 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN112566644A (zh) |
WO (1) | WO2020015264A1 (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101626772A (zh) * | 2007-03-22 | 2010-01-13 | 奥西里斯治疗公司 | 间充质干细胞及其用途 |
CN103191154A (zh) * | 2012-01-06 | 2013-07-10 | 上海交通大学医学院 | 充质干细胞及其提取方法在制备银屑病的药物中的应用 |
CN103585177A (zh) * | 2012-08-13 | 2014-02-19 | 首都医科大学附属北京口腔医院 | 间充质干细胞和基因修饰的间充质干细胞的用途 |
CN106062184A (zh) * | 2014-01-27 | 2016-10-26 | 首都医科大学附属北京口腔医院 | 牙源性干细胞和基因修饰的牙源性干细胞的用途 |
CN106492194A (zh) * | 2016-11-30 | 2017-03-15 | 广州赛莱拉干细胞科技股份有限公司 | 一种干细胞外泌体制剂及其制备方法和应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1657101A (zh) * | 2004-02-19 | 2005-08-24 | 中国人民解放军军事医学科学院放射医学研究所 | 肝细胞生长因子基因修饰骨髓间质干细胞在心肌缺血治疗中的应用 |
JP6781973B2 (ja) * | 2016-01-29 | 2020-11-11 | 学校法人東京女子医科大学 | 腎臓病進行抑制細胞シート組成物、その製造方法、及び、それを用いた腎臓病進行抑制方法 |
-
2018
- 2018-11-22 WO PCT/CN2018/116834 patent/WO2020015264A1/zh active Application Filing
- 2018-11-22 CN CN201880093954.8A patent/CN112566644A/zh active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101626772A (zh) * | 2007-03-22 | 2010-01-13 | 奥西里斯治疗公司 | 间充质干细胞及其用途 |
CN103191154A (zh) * | 2012-01-06 | 2013-07-10 | 上海交通大学医学院 | 充质干细胞及其提取方法在制备银屑病的药物中的应用 |
CN103585177A (zh) * | 2012-08-13 | 2014-02-19 | 首都医科大学附属北京口腔医院 | 间充质干细胞和基因修饰的间充质干细胞的用途 |
CN106062184A (zh) * | 2014-01-27 | 2016-10-26 | 首都医科大学附属北京口腔医院 | 牙源性干细胞和基因修饰的牙源性干细胞的用途 |
US20160348076A1 (en) * | 2014-01-27 | 2016-12-01 | Beijing Stomatology Hospital, Capital Medical University | The Usage of Odontogenic Stem Cells and Genetically Modified Odontogenic Stem Cells |
CN106492194A (zh) * | 2016-11-30 | 2017-03-15 | 广州赛莱拉干细胞科技股份有限公司 | 一种干细胞外泌体制剂及其制备方法和应用 |
Non-Patent Citations (1)
Title |
---|
范瑛等: "基于干细胞探讨开通玄府、补肾培元法治疗银屑病", 《北京中医药》 * |
Also Published As
Publication number | Publication date |
---|---|
WO2020015264A1 (zh) | 2020-01-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Allakhverdi et al. | CD34+ hemopoietic progenitor cells are potent effectors of allergic inflammation | |
US8808682B2 (en) | Method for inducing migration of adipose-derived adult stem cells | |
Zhang et al. | SDF-1 mediates mesenchymal stem cell recruitment and migration via the SDF-1/CXCR4 axis in bone defect | |
JP6306025B2 (ja) | 腫瘍の処置における使用のための子癇前症胎盤間葉系幹細胞馴化培地 | |
KR102360077B1 (ko) | 탯줄 유래 중간엽 줄기세포로부터 분리된 엑소좀을 포함하는 피부 개선용 화장료 조성물 | |
CN111518757A (zh) | 一种免疫抑制或抗炎功能增强型pd-l1阳性间充质干细胞、诱导试剂盒及应用 | |
CN109674819B (zh) | 胎盘间充质干细胞制剂及其治疗硬化病的用途 | |
CA3106188A1 (en) | Mitochondrial augmentation therapy with stem cells enriched with functional mitochondria | |
CN112587720A (zh) | 一种cgf和脐带间充质干细胞外泌体混合物、制备及应用 | |
CN116904394A (zh) | 一种抗炎性间充质干细胞来源外泌体的制备方法及其应用 | |
CN111944748A (zh) | 一种用于治疗心肌梗死的高表达il-10的人脂肪间充质干细胞外泌体及其用途 | |
KR101843952B1 (ko) | 지방조직-유래 기질혈관분획의 분리 방법 | |
Myneni et al. | Mesenchymal stromal cells from infants with simple polydactyly modulate immune responses more efficiently than adult mesenchymal stromal cells | |
KR20150088374A (ko) | 인체 지방-유래 줄기세포를 이용한 주요 세포성장인자를 포함하는 줄기세포 배양액 및 아토피 개선 화장료 조성물 | |
CN106011074B (zh) | 一种高表达cxcr5的间充质干细胞及其制备和用途 | |
CN112566644A (zh) | 牙源干细胞及其用途 | |
WO2022056991A1 (zh) | 脐带来源的间充质干细胞及其制备方法和应用 | |
CN113827618A (zh) | 干细胞条件培养基在制备用于治疗炎症性皮肤的药物中的用途 | |
US20220211766A1 (en) | Selection of fibroblast donors for optimization of allogeneic fibroblast-mediated regeneration | |
CN109749981B (zh) | 人源脂肪干细胞来源的肝细胞样细胞及其制备方法和应用 | |
US20220160777A1 (en) | Method for treating atopic dermatitis using monoclonal stem cells | |
KR101423659B1 (ko) | 초기 분화된 양수 줄기세포를 포함하는 요실금 치료제 | |
JP2024520415A (ja) | 線維芽細胞及び線維芽細胞由来産物を用いる脱毛症治療のための方法及び組成物 | |
US20200038451A1 (en) | Stem cells as an individualized maternal therapy for prevention of prematurity | |
Heidari et al. | Promotion of proliferation of murine hematopoietic stem cells by growth factors in murine amniotic fluid |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Meng Hongfang Inventor before: Meng Hongfang Inventor before: Wang Hua Inventor before: Wu Zuze Inventor before: Tang Zhongxiong |
|
CB03 | Change of inventor or designer information | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210326 |
|
RJ01 | Rejection of invention patent application after publication |