CN112516023A

CN112516023A - Anti-aging skin care product containing saussurea involucrate cell water extract

Info

Publication number: CN112516023A
Application number: CN202011449842.7A
Authority: CN
Inventors: 刘汉石; 黄利; 金银兵
Original assignee: Dalian Practical Biotechnology Co ltd
Current assignee: Dalian Practical Biotechnology Co ltd
Priority date: 2020-12-09
Filing date: 2020-12-09
Publication date: 2021-03-19

Abstract

The invention discloses an anti-aging skin care product containing a saussurea involucrate cell water extract and a preparation method thereof, belonging to the technical field of cosmetics, and the anti-aging skin care product containing the saussurea involucrate cell water extract comprises the following components: saussurea involucrate syringin, saussurea involucrate 1, 5-O-dicaffeoylquinic acid, saussurea involucrate adenosine and saussurea involucrate chlorogenic acid, carbomer, butanediol, glycerol, xanthan gum, sodium polyglutamate, sodium hyaluronate, EDTA-2NA, dipotassium glycyrrhizinate, PEG-6 stearate, ceteth-20 steareth-20, caprylic/capric triglyceride, polydimethylsiloxane, cyclopentadecyldimethicone, tocopheryl acetate, sodium acrylate/sodium acryloyldimethyl taurate copolymer polysorbate-80 sorbitan oleate, isohexadecane hydro-oleate, arginine, methylparaben, propylparaben, phenoxyethanol ethylhexyl glycerol, essence. The anti-aging skin care product containing the saussurea involucrate cellular water extract can effectively ensure the activity of the saussurea involucrate cellular water extract, and can better repair skin barriers by matching with various skin maintenance components to achieve the aim of anti-aging.

Description

Anti-aging skin care product containing saussurea involucrate cell water extract

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to an anti-aging skin care product containing a cell water extract of saussurea involucrate.

Background

Skin aging is mainly divided into two forms of natural aging and photoaging, and natural aging is mainly caused by genetic factors and is obviously characterized by the appearance of wrinkles and the laxity of skin. Photoaging refers to the accumulation of ultraviolet damage during skin aging, and is the result of the combined action of natural aging and ultraviolet radiation. Skin aging is mainly characterized by the deterioration of the protective ability and the regulating ability of skin to the body, appearance changes such as skin color, form, color and luster and the like. With aging, the exposed part of skin is coarse and elastic, wrinkles are deepened and thickened, irregular pigmentation, vasodilation, epidermal keratosis, dermal elasticity fibrosis, degradation product accumulation and the like.

With the improvement of living standard of people, anti-aging becomes a persistent theme, and the market of anti-aging cosmetics is rapidly developed. Although various anti-aging cosmetics are full in the existing market, the requirements of different social people cannot be met.

The saussurea involucrate is a rare Chinese herbal medicine commonly used in folk in high and cold areas in the west of China, is a plant of the genus saussurea in the family of Compositae, and mainly grows on the mountain river slope with the altitude of 4800-. Saussurea involucrate has a long history of application, is particularly used as a medicine, mainly contains flavone, sesquiterpene lactone, coumarin, organic acid, polysaccharide and the like, and has biological activities of resisting oxidation, resisting rheumatism, easing pain, regulating cardiovascular system, resisting cancer and the like. Due to the fact that excessive mining and digging are conducted in recent years, the number of wild saussurea involucrate is greatly reduced, and the use of the wild saussurea involucrate is limited. The saussurea involucrate cultured by the plant cell culture technology has the characteristics of low use cost and high efficacy content. The saussurea involucrate cell is obtained by using a plant cell culture technology (the specific culture method is shown as ZL200510115902.0), the color and the shape of the saussurea involucrate cell are not completely different from those of the saussurea involucrate, the contents of flavone, polysaccharide, chlorogenic acid and syringin in the culture of the saussurea involucrate are all far higher than those of the wild saussurea involucrate, and in addition, the saussurea involucrate cell does not contain a small amount of toxic components of colchicine in the saussurea involucrate.

Disclosure of Invention

In order to solve the technical problems in the background art, the invention discloses an anti-aging skin care product containing a saussurea involucrate cell water extract, which has the effects of better preserving active ingredients, resisting oxidation, removing free radicals, brightening skin color, preserving moisture, lightening fine lines, improving skin quality and the like, can exert the anti-aging effect of the saussurea involucrate cell water extract and cannot cause anaphylactic reaction.

The technical scheme of the invention is as follows;

an anti-aging skin care product containing saussurea involucrate cell water extract comprises the following components by weight percent: 0.0001 to 1 percent of saussurea involucrate syringin, 0.001 to 1 percent of 1, 5-O-dicaffeoylquinic acid of the saussurea involucrate, 0.00005 to 1 percent of adenosin of the saussurea involucrate and 0.001 to 1 percent of chlorogenic acid of the saussurea involucrate.

Preferably, the anti-aging skin care product of the snow lotus herb cell water extract also comprises the following components; carbomer, butanediol, glycerol, xanthan gum, sodium hyaluronate, EDTA-2Na, PEG-6 stearate, ceteth-20 steareth-20, caprylic/capric triglyceride, polydimethylsiloxane, cyclopentadimethylsiloxane, tocopheryl acetate, sodium acrylate/sodium acryloyldimethyl taurate copolymer polysorbate-80 sorbitan oleate hydroisohexadecane, arginine, methylparaben, propylhydroxybenzoate, phenoxyethanol ethylhexyl glycerol and essence.

Preferably, the anti-aging skin care product of the saussurea involucrate cell water extract also comprises the following components in percentage by weight; 0.05-0.3% of carbomer, 2-6% of butanediol, 2-5% of glycerol, 0.05-0.2% of xanthan gum, 0.01-0.1% of sodium hyaluronate, 0.03-0.1% of EDTA-2NA, 0.03-0.1% of PEG-6 stearate, 1-3% of ceteth-20 and steareth-20 (the weight ratio is 6.5:2.5:1), 1-4% of caprylic/capric triglyceride, 2-5% of polydimethylsiloxane, 3-10% of cyclopenta-dimethylsiloxane, 0.05-0.5% of tocopheryl acetate, 0.04-0.24% of sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, 0.5-3% of sorbitan oleate, 0.5-0.14% of water and isohexadecane (the weight ratio is 40:10:2.5:22.5:25), 0.04-0.24% of arginine, 0.15-0.2% of methyl hydroxybenzoate, 0.1-0.14% of propyl hydroxybenzoate, and 0.4% of phenoxyethanol and ethylhexylglycerol (the weight ratio is 9:1, 0.01 to 0.1 percent of essence and the balance of water.

Preferably, the anti-aging skin care product of the snow lotus herb cell water extract also comprises the following components: sodium polyglutamate, dipotassium glycyrrhizinate, squalane, cetostearyl alcohol and jojoba oil.

Preferably, the anti-aging skin care product containing the saussurea involucrate cell water extract also comprises the following components in percentage by weight: 0.01 to 0.1 percent of sodium polyglutamate, 0.05 to 0.2 percent of dipotassium glycyrrhizinate, 1 to 3 percent of squalane, 0.5 to 2 percent of cetyl stearyl alcohol, 1 to 3 percent of jojoba oil and the balance of water.

Preferably, the preparation method of the extract of the saussurea involucrate syringin, the 1, 5-O-dicaffeoylquinic acid of the saussurea involucrate, the adenosine of the saussurea involucrate and the chlorogenic acid of the saussurea involucrate comprises the following steps;

a washing fresh herba Saussureae Involueratae cells with pure water

B, putting the cleaned saussurea involucrate cells into a homogenizer, adding pure water, wherein the weight ratio of the saussurea involucrate cells to the water is 1:1-1:10, homogenizing:

c, filtering the homogenate obtained in the step B by using a filter membrane with the aperture of 0.45um, subpackaging and storing at-5 ℃.

Preferably, in step A, the washing times are 3 times, and the amount of pure water used in each time is 5 times of the weight of the saussurea involucrate cells.

The preparation method of the anti-aging skin care product containing the saussurea involucrate cell water extract comprises the following steps:

(1) adding pure water into a water phase pot, starting rapid stirring, slowly adding carbomer, (butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, premixing), stirring sufficiently to make it fully dispersed and swollen, adding EDTA-2Na, dipotassium glycyrrhizinate and methyl hydroxybenzoate, and supplementing 1.5% pure water;

(2) heating the water phase pot to 80-90 ℃, adding the mixture of the raw materials PEG-6 stearate, ceteth-20 and steareth-20, and stirring for 10-30 min at the constant temperature; simultaneously carrying out oil phase pot operation:

(3) sequentially adding caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, propyl hydroxybenzoate, squalane, cetyl stearyl alcohol and jojoba oil into an oil pan, heating to 80-90 ℃, and keeping the temperature for 10-30 min for later use;

(4) preheating a vacuum emulsifying pot to 35-45 ℃, vacuumizing, pumping the water phase into the vacuum emulsifying pot, starting rapid stirring, homogenizing at 1500-2500 rpm for 3-30 min, pumping an oil phase, homogenizing at 2500-3500 rpm for 5-30 min, keeping the temperature and stirring for 10-20 min, cooling, and transferring to the next operation;

(5) cooling the vacuum emulsifying pot to 65-75 ℃, adding (sodium acrylate/acryloyl dimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water, isohexadecane and cyclopentadimethylsiloxane, pre-mixing), homogenizing at 2500-3500 rpm for 5-30 min, keeping the temperature, stirring for 10-20 min, and continuously cooling;

(6) cooling the vacuum emulsification pot to 50 ℃, adding the raw materials (arginine and water which are dissolved in advance) into the vacuum emulsification pot, continuously stirring and cooling, cooling to 45 ℃, adding the extracting solutions of phenoxyethanol, ethylhexylglycerin, essence, saussurea involucrate syringin, saussurea involucrate 1, 5-O-dicaffeoylquinic acid, saussurea involucrate adenosine and saussurea involucrate chlorogenic acid into the vacuum emulsification pot one by one, homogenizing at 1500-2500 rpm for 1-10 min, and continuously stirring and cooling; cooling to 38 deg.C, and discharging.

Compared with the prior art, the invention has the following beneficial effects:

1. the anti-aging skin care product containing the snow lotus herb cell water extract can effectively ensure the activity of the snow lotus herb cell water extract, and can better repair skin barriers by matching with various skin maintenance components to achieve the aim of anti-aging.

2. The invention adopts the combination of homogenization and ultrafiltration to extract the saussurea involucrate cell water extract, the combination of the homogenization and the ultrafiltration ensures the extraction sufficiency, and the extract with proper grain diameter can be obtained without evaporation and drying.

3. The saussurea involucrate cell water extract has the functions of absorbing ultraviolet rays, resisting radiation, resisting inflammation, easing pain, resisting aging and the like.

Detailed Description

The present invention is described in detail below with reference to examples, but the embodiments of the present invention are not limited thereto, and it is obvious that the examples in the following description are only some examples of the present invention, and it is obvious for those skilled in the art to obtain other similar examples without inventive exercise and falling into the scope of the present invention.

Example 1

A method for preparing antiaging skin care product containing herba Saussureae Involueratae cell water extract comprises the following steps:

(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding PEG-6 stearate, cetyl polyether-20 and stearyl polyether-20, stirring for 10 min; simultaneously carrying out oil phase pot operation:

(3) sequentially adding caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, propyl hydroxybenzoate, squalane, cetostearyl alcohol and jojoba oil into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;

(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;

(5) cooling to 75 deg.C in vacuum emulsifying pot, adding (sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water, isohexadecane and cyclopentadecyldimethyl siloxane, mixing in advance), homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;

(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding raw materials (arginine and water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexylglycerin, essence, Tianshan herba Saussureae Involueratae syringin, Tianshan herba Saussureae Involueratae 1, 5-O-dicaffeoylquinic acid, Tianshan herba Saussureae Involueratae adenosine and Tianshan herba Saussureae Involueratae chlorogenic acid extract into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, continuously stirring for 1min and cooling; cooling to 38 deg.C, and discharging.

The preparation method of the saussurea involucrate syringin, the 1, 5-O-dicaffeoylquinic acid of the saussurea involucrate, the adenosin of the saussurea involucrate and the chlorogenic acid of the saussurea involucrate comprises the following steps;

a washing fresh herba Saussureae Involueratae cells with pure water

The content of effective substances in the cell water extract of saussurea involucrate is respectively 0.001% of syringin, 0.0232% of 1, 5-O-dicaffeoylquinic acid, 0.0007% of adenosine of saussurea involucrate and 0.015% of chlorogenic acid of saussurea involucrate detected by high performance liquid chromatography.

The components of the anti-aging skin care product containing the water extract of the saussurea involucrate cell are shown in table 1.

Table 1: the component composition of the restorative anti-aging skin care product of example 1;

example 2

(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding raw materials (arginine and water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexylglycerin, essence, herba Saussureae Involueratae syringin, herba Saussureae Involueratae 1, 5-O-dicaffeoylquinic acid, herba Saussureae Involueratae adenosine and herba Saussureae Involueratae chlorogenic acid extractive solution into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, and continuously stirring and cooling for 1 min; cooling to 38 deg.C, and discharging.

a washing fresh herba Saussureae Involueratae cells with pure water

The components of the anti-aging skin care product containing the water extract of the saussurea involucrate cell are shown in table 2.

Table 2: the composition of the components of the restorative anti-aging skin care product of example 2;

example 3

a washing fresh herba Saussureae Involueratae cells with pure water

The components of the anti-aging skin care product containing the water extract of the saussurea involucrate cell are shown in table 3.

Table 3: the component composition of the restorative anti-aging skin care product of example 3;

example 4

(1) adding pure water into a water phase pot, starting rapid stirring, slowly adding carbomer, (butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, premixing) and fully stirring to fully disperse and swell, adding EDTA-2Na, dipotassium glycyrrhizinate and methyl hydroxybenzoate, and supplementing 1.5% of pure water;

a washing fresh herba Saussureae Involueratae cells with pure water

The components of the anti-aging skin care product containing the water extract of the saussurea involucrate cell are shown in table 4.

Table 4: the component composition of the restorative anti-aging skin care product of example 4;

comparative example 1

A preparation method of an anti-aging skin care product comprises the following steps:

(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding raw materials (arginine and water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring, cooling to 45 deg.C, adding phenoxyethanol, ethylhexylglycerin, and essence into the vacuum emulsifying pot one by one, homogenizing at 1800rpm for 1min, and continuously stirring and cooling; cooling to 38 deg.C, and discharging.

Table 5: the components of the repair anti-aging skin care product of comparative example 1;

test examples

Evaluation of Effect of anti-aging skin-Care products

The test method comprises the following steps: in the same area, 200 female volunteers with aged skin were screened at the same time, aged 35-50 years, and the average age was 43 years.

And (3) inclusion standard: the skin around the eyes has mild or moderate wrinkles, dry skin, reduced elasticity, wrinkles, pigmentation, dull skin color and other skin aging phenomena; and randomly dividing the skin care products into 5 groups, wherein 40 persons in each group and 5 groups of volunteers respectively correspond to the skin care products in the embodiments 1-4 and the comparative example 1, each group of volunteers uses the corresponding test product as a unique eye care product, after cleaning the face in the morning and evening, the corresponding test product is smeared around the eyes and is gently pressed until the eye is absorbed, the test time is 60 days, the volunteers participating in the test feed back the change condition and the use feeling of the eye skin every day, the condition is recorded, the data is summarized, and the effect evaluation is carried out on the test subjects.

Skin aging evaluation: evaluated by observing the subject's test area (crow's feet, glabellar lines, forehead wrinkles).

And (4) judging the standard:

has no wrinkles

Mild: 2-3 wrinkles, length less than 1cm

Medium: 3-6 wrinkles, length less than 2cm

And (3) severe degree: has several wrinkles of length more than 3cm and simultaneously has shallow wrinkles

Table 6: evaluating the skin aging of each group of testees after the test is finished;

group of	Without wrinkles (example)	Slight (example)	Moderate (example)	Severe (example)
					Example 1	29	10	1	0
Example 2	32	7	1	0
					Example 3	35	5	0	0
Example 4	28	10	2	0
					Comparative example	16	14	6	4

Therefore, the anti-aging skin care product containing the saussurea involucrate cell water extract can relieve wrinkles around eyes, repair skin barriers and achieve the anti-aging purpose.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti-aging skin care product containing a saussurea involucrate cell water extract is characterized by comprising the following components in percentage by weight: 0.0001 to 1 percent of saussurea involucrate syringin, 0.001 to 1 percent of 1, 5-O-dicaffeoylquinic acid of the saussurea involucrate, 0.00005 to 1 percent of adenosin of the saussurea involucrate and 0.001 to 1 percent of chlorogenic acid of the saussurea involucrate.

2. The anti-aging skin care product of claim 1, further comprising the following components in percentage by weight: 0.05-0.3% of carbomer, 2-6% of butanediol, 2-5% of glycerol, 0.05-0.2% of xanthan gum, 0.01-0.1% of sodium hyaluronate, 0.03-0.1% of EDTA-2Na, 1-3% of a mixture of PEG-6 stearate, ceteth-20 and steareth-20, 1-4% of caprylic/capric triglyceride, 2-5% of polydimethylsiloxane, 3-10% of cyclopenta dimethyl siloxane, 0.05-0.5% of tocopheryl acetate, 0.5-3% of a mixture of sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water and isohexadecane, 0.04-0.24% of arginine, 0.15-0.2% of methyl hydroxybenzoate, 0.1-0.14% of propyl hydroxybenzoate, 0.4-0.5% of a mixture of phenoxyethanol and ethylhexylglycerol, 0.01 to 0.1 percent of essence and the balance of water;

wherein the weight ratio of PEG-6 stearate, cetyl polyether-20 and stearyl polyether-20 is 6.5:2.5: 1; the weight ratio of the sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water and isohexadecane is 40:10:2.5:22.5: 25; the weight ratio of the phenoxyethanol to the ethylhexyl glycerin is 9: 1.

3. The anti-aging skin care product according to claim 1 or 2, further comprising the following components in percentage by weight: 0.01 to 0.1 percent of sodium polyglutamate, 0.05 to 0.2 percent of dipotassium glycyrrhizinate, 1 to 3 percent of squalane, 0.5 to 2 percent of cetyl stearyl alcohol and 1 to 3 percent of jojoba oil.

4. The anti-aging skin care product as claimed in claim 3, wherein the preparation method of the saussurea involucrate syringin, the saussurea involucrate 1, 5-O-dicaffeoylquinic acid, the saussurea involucrate adenosine and the saussurea involucrate chlorogenic acid comprises the following steps;

a, washing fresh saussurea involucrate cells by using pure water;

and C, filtering the homogenate obtained in the step B by using a filter membrane with the aperture of 0.45um to obtain the product.

5. The anti-aging skin care product as claimed in claim 4, wherein in the step A, the washing times are 3 times, and the usage amount of pure water is 5 times of the weight of the saussurea involucrate cells.

6. The anti-aging skin care product according to claim 5, wherein the skin care product is a cream, an eye cream, an emulsion, a essence, a skin lotion, a mask or a face lotion.

7. The method for preparing an anti-aging skin care product according to any one of claims 1 to 6, which comprises the steps of;

(1) adding pure water into a water phase pot, stirring, adding carbomer, butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, swelling, and adding EDTA-2Na, dipotassium glycyrrhizinate and methylparaben;

(2) heating the water phase pot to 80-90 ℃, adding a mixture of PEG-6 stearate, ceteth-20 and steareth-20, and stirring for 10-30 min:

(3) sequentially adding caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, propyl hydroxybenzoate, squalane, cetyl stearyl alcohol and jojoba oil into an oil pan, heating to 80-90 ℃, and keeping the temperature for 10-30 min;

(4) preheating a vacuum emulsifying pot to 35-45 ℃, pumping the water phase into the vacuum emulsifying pot, starting stirring, starting homogenizing at 1500-2500 rpm for 3-30 min, pumping the oil phase, homogenizing at 2500-3500 rpm for 5-30 min, keeping the temperature, stirring for 10-20 min, and cooling;

(5) cooling the vacuum emulsification pot to 65-75 ℃, adding a mixture of sodium acrylate/acryloyl dimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water, isohexadecane and cyclopentadimethylsiloxane, homogenizing at 2500-3500 rpm for 5-30 min, and stirring at the constant temperature for 10-20 min;

(6) cooling the mixture to 48-52 ℃ in a vacuum emulsifying pot, adding an arginine aqueous solution, stirring and cooling the mixture to 42-45 ℃, adding a mixture of phenoxyethanol and ethylhexylglycerin, essence, saussurea involucrate syringin, saussurea involucrate 1, 5-O-dicaffeoylquinic acid, saussurea involucrate adenosine and saussurea involucrate chlorogenic acid extract, homogenizing the mixture at 1500-2500 rpm for 1-10 min, stirring and cooling the mixture to below 38 ℃, and discharging the product.

8. The method of claim 7, wherein the butylene glycol, glycerin, xanthan gum, sodium hyaluronate and sodium polyglutamate are premixed in step (1), and then added to the aqueous phase pot.

9. The method of claim 8, wherein the sodium acrylate/sodium acryloyldimethyl taurate copolymer, polysorbate-80, sorbitan oleate, water, isohexadecane and cyclopentadimethylsiloxane are pre-mixed in step (5), and then added to a vacuum emulsification vessel.