CN112472797A - Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine - Google Patents

Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine Download PDF

Info

Publication number
CN112472797A
CN112472797A CN202011465980.4A CN202011465980A CN112472797A CN 112472797 A CN112472797 A CN 112472797A CN 202011465980 A CN202011465980 A CN 202011465980A CN 112472797 A CN112472797 A CN 112472797A
Authority
CN
China
Prior art keywords
skin
bonobo
frog
photoaging
cathelicidin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202011465980.4A
Other languages
Chinese (zh)
Other versions
CN112472797B (en
Inventor
木丽仙
武静
杨海龙
边慧
米鍇
沈艳
冯贵珠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kunming Medical University
Original Assignee
Kunming Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kunming Medical University filed Critical Kunming Medical University
Priority to CN202011465980.4A priority Critical patent/CN112472797B/en
Publication of CN112472797A publication Critical patent/CN112472797A/en
Application granted granted Critical
Publication of CN112472797B publication Critical patent/CN112472797B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/18—Antioxidants, e.g. antiradicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Abstract

The invention discloses an application of a bonobo frog skin repair peptide cathelicidin-NV in preparing skin anti-photoaging and oxidation medicaments, in particular to an application of a bonobo frog (Nanora ventriculriputa) natural multifunctional antioxidant peptide cathelicidin-NV in removing free radicals in vivo and protecting skin against photoaging and oxidation, belonging to the field of biomedicine. The bonobo frog skin repair peptide cathelicidin-NV is a circular polypeptide encoded by a bonobo frog defense peptide gene of a published special species of the amphibian China. Experiments prove that the natural multifunctional antioxidant peptide cathelicidin-NV of the rana obovata has strong effects of eliminating in-vivo free radicals and resisting photoaging and antioxidant activity of skin, can be applied to preparation of in-vivo free radical eliminating medicines, skin antioxidant protection medicines and skin photoaging resisting treatment medicines, and also can be used for preparation of cosmetics.

Description

Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine
Technical Field
The invention relates to an application of a bonobo frog skin repair peptide cathelicidin-NV in preparing skin anti-photoaging and oxidation medicaments, in particular to an application of a natural multifunctional antioxidant peptide, namely a bonobo frog (rana ventriculturi) skin repair peptide cathelicidin-NV in preparing skin anti-photoaging treatment medicaments, skin anti-oxidation protection medicaments, in-vivo free radical scavenging medicaments and cosmetics, belonging to the field of biomedicine.
Background
Free radicals are a group or atom of atoms with one or several unpaired electrons, which are generated in the metabolic process of organisms and can exist independently, have high oxidation activity, are necessary for life support, and are also potential killers of biological macromolecules, cells and biological tissues. The generation of free radicals can be caused by many dysfunctions and diseases of the body such as phagocytosis, detoxification, inflammation, tumor, aging, radiation damage, etc. The timely elimination of excess free radicals in the body is an important component of the immune regulation of the body. The antioxidant polypeptide has rapid and strong free radical scavenging capacity, can protect the skin to the maximum extent, and can be damaged by free radicals induced by sunlight, ultraviolet rays and the like as little as possible.
Skin photoaging (Skin photoaging) refers to the characteristic changes in Skin structure and function resulting from repeated exposure of the Skin to ultraviolet light, characterized by premature aging of the Skin, multiple wrinkles, rough leathery appearance, pigmentation, telangiectasia, associated precancerous lesions and malignancies. Because photoaging not only seriously affects the appearance of the skin of a patient, greatly reduces the quality of life of the patient, but also induces skin malignant tumors (such as malignant melanoma, basal cell carcinoma, squamous cell carcinoma and the like), in recent years, because of the damage of the atmospheric ozone layer, the incidence of the skin photoaging caused by the radiation is increased gradually, in 2015, the huge medical cost for treating the severe skin photoaging and the skin cancer caused by the severe skin photoaging is over 291 hundred million dollars each year, wherein the U.S. is as high as 114 million dollars, so how to effectively prevent and treat the skin photoaging caused by ultraviolet rays, and finding a novel efficient antioxidant medicament which is safe, low in toxicity and low in cost in the process of preventing and treating the photoaging becomes an urgent problem to be solved.
Amphibians are the most important resource in the discovery of bioactive polypeptide resources, have exposed skins, and can secrete various bioactive polypeptides with novel molecular structures and complex and diverse functions in order to resist various severe environments in the environment. The active polypeptides are widely involved in various physiological activities of organisms, and have various pharmacological activities, such as antimicrobial, anti-tumor, antioxidant, immunoregulation, wound repair, analgesia and the like. At present, the screening of some pharmacological active monomer compounds from the skin of amphibians is the hotspot of the new drug invention. According to the literature reports at home and abroad, different active polypeptides have been separated from various biological sources, and some active polypeptides enter the clinical stage. The rana ventricosa (Nanorana ventrriparia), which is an amphibian of the genus Bombarus of the order Anophialae, is a species specific to China and a species specific to the species of Qinghai-Tibetan plateau, has a much harsher living environment than other Ranidae animals, and has the characteristics of long-term continuous sunlight irradiation, strong ultraviolet radiation, cold plateau, low pressure and oxygen deficiency, dryness and strong wind climate. The specific harsh ecological environment makes the internal and external stressors and external microbial attacks on the amphibians different. The special high-altitude strong ultraviolet environment necessarily endows the species with special substances for adapting to the severe environment.
China patent CN108484747B discloses a Japanese frog skin repair peptide cathelicidin-NV and application thereof in preparing a medicine for promoting skin wound repair, and the application relates to the effects of resisting photoaging of skin, resisting oxidation, protecting and removing free radicals in vivo.
Disclosure of Invention
Aiming at the application of the cathelicidin-NV peptide in the skin secretion of the squash frog in the prior art in preparing the medicine for promoting the repair of skin wounds, the inventor finds that the cathelicidin-NV peptide also has extremely strong antioxidant activity and skin anti-photoaging activity through further research and experiments, and shows that the cathelicidin-NV peptide not only has the activity of promoting the repair of the skin, but also has the activity of resisting oxidation and skin photoaging, so the cathelicidin-NV peptide can be considered to be multifunctional antioxidant peptide.
The invention aims to provide a new application of cathelicidin-NV in the aspects of strong skin photoaging resistance, skin oxidation resistance protection and in vivo free radical scavenging on the basis of the application of cathelicidin-NV in promoting skin repair of the ranobovata on the basis of the prior art.
The invention discloses a bonobo frog skin repair peptide cathelicidin-NV which is a cyclic polypeptide encoded by a bonobo frog defense peptide gene of a specific species of amphibian China, and according to the disclosure of a bonobo frog skin repair peptide cathelicidin-NV and a gene and application thereof (CN108484747B), the bonobo frog skin repair peptide cathelicidin-NV consists of a pair of cyclic polypeptides with intramolecular disulfide bonds formed by a seventh cysteine and a twelfth cysteine, the molecular weight is 2845.9 daltons, and the isoelectric point 9.90 polypeptide complete sequence primary structure (amino acid sequence SEQ ID NO:1) is as follows:
Figure BDA0002832776420000031
ARGKKECKDDRCRLLMKRGSFSYV (24aa), the seventh and twelfth cysteines of which constitute a pair of intramolecular disulfide bonds.
The content of the invention comprises:
application of the bonobo frog skin repair peptide cathelicidin-NV in preparing medicines for eliminating free radicals in vivo.
Application of the Japanese frog skin repair peptide cathelicidin-NV in preparing medicine for treating skin photoaging is provided.
Application of a Japanese frog skin repair peptide cathelicidin-NV in preparing skin antioxidant protection medicines.
The skin anti-photoaging treatment medicine and/or the skin anti-oxidation protection medicine are used for preparing cosmetics.
The invention has the beneficial effects that: provides a new application of cathelicidin-NV in the aspects of strong skin anti-photoaging, skin oxidation protection and in vivo free radical scavenging. The Japanese frog skin repair peptide has strong antioxidant activity and skin anti-photoaging effect, and can be used for preparing skin antioxidant protection medicine, in vivo free radical scavenging medicine, skin anti-photoaging therapeutic medicine and cosmetics. The Japanese frog skin repair peptide has the advantages of simple sequence and convenient synthesis, and provides better application prospects for preparing skin antioxidant protection medicines, in-vivo free radical scavenging medicines, skin anti-photoaging treatment medicines and cosmetics.
Drawings
FIG. 1 is a graph of the radical clearance of the bonobo frog skin repair peptide cathelicidin-NV of example 1 at different concentrations and times of action.
FIG. 2 is a graph showing the results that the bonobo frog skin repair peptide cathelicidin-NV of example 2 has the effect of scavenging Reactive Oxygen Species (ROS) in cells.
FIG. 3 is a graph showing the effect of the bonobo frog skin repair peptide cathelicidin-NV of example 3 on its potent anti-photoaging effect on skin.
In fig. 2 and 3, UVB-represents uv-free UVB irradiation; UVB + represents ultraviolet UVB irradiation; UVB + cathelicidin-NV stands for ultraviolet UVB irradiation, with cathelicidin-NV treatment given.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
Example 1: antioxidation and free radical scavenging effects of Japanese butterfrog skin repair peptide cathelicidin-NV
The ABTS + scavenging method was used in this example to examine the antioxidant and free radical scavenging effects of the bonobo frog skin repair peptide.
ABTS+It is a very stable blue-green radical cation that turns to colorless ABTS in the presence of an antioxidant with hydrogen donating ability. ABTS elimination by antioxidant peptide+The ability of (c) was determined by the change in absorbance at 734 nm. Antioxidant capacity and ABTS of samples+The residual amount of. ABTS+The residual amount (%) was calculated according to the following formula:
％ABTS+·R＝[(ABTS+·)T/(ABTS+·)t＝0]×100
ABTS+t is ABTS+734nm light absorption after incubation with the sample for 10min in the absence of light; ABTS+0 is ABTS+The initial 734nm light absorption. The experimental method comprises the following steps:
preparation of PBS stock solution of ABTS, the concentration is 2 mM. II, K2S2O8Was prepared in a concentration of 70 mM. ABTS+Preparation of (1): ABTS stock solution and K before use2S2O8The solution is mixed according to the volume ratio of 250:1, and is protected from light for 15-16h at room temperature. Diluting ABTS with PBS before experiment+Solution, so that its a734 is between 0.8 ± 0.030. Draw diluted ABTS+Mu.l of the solution was added with 2. mu.l of a sample to be measured (concentration: 0.5 to 10mg/ml), and the mixture was left at room temperature for 10min to measure the light absorption value at a wavelength of 734 nm. The PBS is zeroed.
In all the above tests, a sample dissolving solution (ultrapure water) was used as a control, and 3 samples were set in parallel in each group.
The results are shown in FIG. 1, and show that the effect on ABTS is increased along with the increase of cathelicidin-NV concentration and action time+The clearance rate is increased, and the clearance efficiency is highest within 1 min. 80 μ g/ml cathelicidin-NV completed more than 90% ABTS at 1min+The clearance of (2) is up to 94.6 in 10min5.4. The result shows that the Japanese frog skin repair peptide cathelicidin-NV has strong effects of eliminating free radicals and resisting oxidation, has the advantages of simple sequence, convenient synthesis and the like, and can be used for preparing medicines for resisting oxidation, protecting skin and eliminating free radicals in vivo.
Example 2: function of bonobo frog skin repair peptide cathelicidin-NV in eliminating active oxygen (ROS) in cell
In the embodiment, the content of ROS in Ultraviolet (UVB) mediated HaCat cells is detected by a flow cytometer, and the in-vivo antioxidant effect of the bonobo frog skin repair peptide is detected.
UVB radiation HaCat can cause oxidative stress of cells and cause the sharp increase of Reactive Oxygen Species (ROS) in the cells, so that the metabolism of the cells is disturbed. We measured UVB-mediated HaCat intracellular ROS content by flow cytometry, and the results are shown in fig. 2: the UVB radiation-treated group was found to significantly upregulate intracellular ROS compared to the normal group (non-UVB radiation-treated group), whereas the butterflybush skin repair peptide cathelicidin-NV significantly reduced intracellular ROS content. The results fully indicate that the cathelicidin-NV can effectively eliminate UVB mediated HACat intracellular ROS, and further prove that the cathelicidin-NV has strong intracellular antioxidation. Example 3: application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of anti-photoaging skin treatment medicine
The hairless mice were randomly divided into UVB-groups, no uv UVB irradiation; UVB + group: ultraviolet UVB irradiation: UVB + cathelicidin-NV group: ultraviolet UVB radiation, and cathelicidin-NV treatment. Coating PBS solution on the back of hairless mouse in UVB-group and UVB + group, coating cathelicidin-NV-group in skin antioxidant peptide dissolved in PBS solution on the back of hairless mouse, placing hairless mouse at 50cm position under UVB light source, irradiating once per day for the first week except normal control group for 5 times, wherein each time of UVB irradiation dose is 100mJ/cm2After 2 weeks, the other groups except the normal control group were irradiated with UVB once every other day at a dose of 200mJ/cm2For a total of 12 weeks.
The results are shown in FIG. 3: after 12 weeks of UVB irradiation, typical skin photoaging phenomena appeared by visual observation of the dorsal exposure area of UVB + group hairless mice: the appearance of the leather is changed by progressive elasticity loss, the wrinkles become thick and deep, and the appearance of the leather is accompanied with photoaging such as desquamation; the skin color of the UVB-group is normal and good in elasticity, and the skin photoaging change of the UVB + cathelicidin-NV group is obviously lighter than that of the UVB + group after the Japanese frog skin repair peptide cathelicidin-NV is given. Further histologically observing the pathological changes of the skin of the hairless mouse photoaging model, obviously thickening the epidermis of the UVB + group, and eliminating the epidermis process and the dermal papilla; dermal fibrosis, disorganization, maldistribution or sparseness. The UVB-group epidermal cells are arranged in order, the dermis layer can see wavy fibrous tissues, the arrangement is ordered, the distribution is uniform, the density is dense, and the cell components are moderate in quantity. When the UVB + cathelicidin-NV group gives the Japanese frog skin repair peptide cathelicidin-NV, the epidermis thickness and collagen fibers are denatured, and the damage degree is lighter than that of the UVB + group.
The experimental results show that: the Japanese frog skin repair peptide cathelicidin-NV has strong skin anti-photoaging effect, obviously inhibits the skin photoaging phenomenon caused by UVB, and obviously inhibits the epidermal layer thickening caused by photoaging, and is shown in figure 3 in detail.

Claims (4)

1. The application of the bonobo frog skin repair peptide cathelicidin-NV in preparing skin antioxidant protection medicaments is characterized in that the bonobo frog skin repair peptide cathelicidin-NV is a cyclic polypeptide encoded by a bonobo frog defense peptide gene of a specific species of amphibian China, a pair of cyclic polypeptides of intramolecular disulfide bonds are formed by a seventh cysteine and a twelfth cysteine, the molecular weight is 2845.9 daltons, and the isoelectric point is 9.90.
2. The application of the bonobo frog skin repair peptide cathelicidin-NV in preparing the skin anti-photoaging treatment medicine, wherein the bonobo frog skin repair peptide cathelicidin-NV is a cyclic polypeptide encoded by a bonobo frog defense peptide gene of a specific species of amphibian China, the cyclic polypeptide of a pair of intramolecular disulfide bonds is formed by the seventh cysteine and the twelfth cysteine, the molecular weight is 2845.9 daltons, and the isoelectric point is 9.90.
3. The application of the bonobo frog skin repair peptide cathelicidin-NV in preparing medicaments for eliminating free radicals in vivo is characterized in that the bonobo frog skin repair peptide cathelicidin-NV is a cyclic polypeptide encoded by a bonobo frog defense peptide gene of a specific species of amphibian China, the cyclic polypeptide of a pair of intramolecular disulfide bonds is formed by the seventh cysteine and the twelfth cysteine, the molecular weight is 2845.9 daltons, and the isoelectric point is 9.90.
4. Use according to claim 1 or 2, characterized in that said anti-photoaging therapeutic and/or antioxidant protective skin agents are used for the preparation of cosmetics.
CN202011465980.4A 2020-12-13 2020-12-13 Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine Active CN112472797B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011465980.4A CN112472797B (en) 2020-12-13 2020-12-13 Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011465980.4A CN112472797B (en) 2020-12-13 2020-12-13 Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine

Publications (2)

Publication Number Publication Date
CN112472797A true CN112472797A (en) 2021-03-12
CN112472797B CN112472797B (en) 2022-04-12

Family

ID=74916560

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011465980.4A Active CN112472797B (en) 2020-12-13 2020-12-13 Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine

Country Status (1)

Country Link
CN (1) CN112472797B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012046922A1 (en) * 2010-10-08 2012-04-12 Korea Advanced Institute Of Science And Technology Novel use of antimicrobial peptides in regeneration of skin cells
US20150232523A1 (en) * 2012-09-19 2015-08-20 Mackay Memorial Hospital Use of pedf-derived polypeptides for preventing and/or ameliorating skin aging
CN106749595A (en) * 2017-02-24 2017-05-31 昆明医科大学 Tree frog defense peptides cathelicidin PP and its gene and application
CN108484747A (en) * 2018-03-22 2018-09-04 昆明医科大学 Japan frog skin repair peptide cathelicidin-NV and its gene and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012046922A1 (en) * 2010-10-08 2012-04-12 Korea Advanced Institute Of Science And Technology Novel use of antimicrobial peptides in regeneration of skin cells
US20150232523A1 (en) * 2012-09-19 2015-08-20 Mackay Memorial Hospital Use of pedf-derived polypeptides for preventing and/or ameliorating skin aging
CN106749595A (en) * 2017-02-24 2017-05-31 昆明医科大学 Tree frog defense peptides cathelicidin PP and its gene and application
CN108484747A (en) * 2018-03-22 2018-09-04 昆明医科大学 Japan frog skin repair peptide cathelicidin-NV and its gene and application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GUIZHU FENG等: "Cathelicidin-NV from Nanorana ventripunctata effectively protects HaCaT cells, ameliorating ultraviolet B-induced skin photoaging", 《PEPTIDES》 *
JING WU等: "A frog cathelicidin peptide effectively promotes cutaneous wound healing in mice", 《BIOCHEMICAL JOURNAL》 *
周庆峰等: "蛙皮活性抗菌肽最新研究进展", 《中国农学通报》 *
杨俊: "高原腹斑倭蛙皮肤分泌物中皮肤修复肽的结构与功能研究", 《中国优秀硕士学位论文全文数据库基础科学辑》 *

Also Published As

Publication number Publication date
CN112472797B (en) 2022-04-12

Similar Documents

Publication Publication Date Title
CN108852894A (en) It is a kind of for repairing the polypeptides in combination of whitening wrinkle-removing
CN109549893B (en) Composition and essence milk with blue light pollution resistance and repair effects and preparation method of essence milk
CN111728917A (en) Skin care product and preparation method thereof
CN111067838A (en) Whitening and freckle-removing composition, whitening and freckle-removing cream and preparation method thereof
CN111704652A (en) Anti-photodamage skin protection active polypeptide OM-GL15 and application thereof
CN104490747B (en) With radiation proof, the composition of light protection defense function and preparation method thereof
CN111388349A (en) Facial mask containing moisturizing and whitening essence and preparation method thereof
CN112472797B (en) Application of Japanese butterfrog skin repair peptide cathelicidin-NV in preparation of skin anti-photoaging and anti-oxidation medicine
KR20100021755A (en) Cosmetic composition for anti-aging of the skin
CN112826778A (en) Polypeptide and plant composition for inhibiting melanin synthesis
CN111632018A (en) Composition containing hydrolyzed algae extract and application thereof
CN102258434A (en) Application of polygonin in preparing sunscreen cosmetics
CN115300432A (en) After-sun repair composition and preparation method and application thereof
CN111067893B (en) Composition and application thereof in preparation of skin repair product
CN114469907A (en) Application of nerol in preparation of product for resisting skin photodamage
CN112402342A (en) Anti-aging cosmetic additive and preparation method thereof
CN113940909A (en) Composition for regulating circadian rhythm and application thereof
CN109925248B (en) A skin care composition containing phloretin and Panax notoginsenosides with skin photoaging resisting effect
CN109939052B (en) Skin care composition with skin photoaging resisting effect
CN105769964A (en) Application of fructus arctii extract in resistance of skin photoage
CA2699676C (en) The use of a photosynthetic cell extract in a cosmetic composition
CN103735566A (en) Application of soracan gum in inhibiting skin injury of ultraviolet ray
CN109718192B (en) Short finger soft coral extract and its use
CN108420056A (en) A kind of composition of beautifying face and moistering lotion and products thereof
Jurek et al. Skin beautification with an ImmunatuRNA® complex: a natural complex based on yeast RNA and marine derivatives on the human skin immunity and microbiome composition

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant