CN105769964A - Application of fructus arctii extract in resistance of skin photoage - Google Patents
Application of fructus arctii extract in resistance of skin photoage Download PDFInfo
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- XOJVHLIYNSOZOO-SWOBOCGESA-N Arctiin Chemical compound C1=C(OC)C(OC)=CC=C1C[C@@H]1[C@@H](CC=2C=C(OC)C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)=CC=2)C(=O)OC1 XOJVHLIYNSOZOO-SWOBOCGESA-N 0.000 title claims abstract description 61
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses application of a fructus arctii extract in resistance of skin photoage. The fructus arctii extract can be used for remarkably resisting an injury function of ultraviolet rays to keratinocytes in a cell level and remarkably protecting the harm of the ultraviolet rays to skins of mice in the animal level, and has significant effect for resisting the ultraviolet rays. The invention provides novel application of the fructus arctii extract in prevention of sunshine injury and photoageing. The fructus arctii extract has development potential, and can be applied to the technical fields of medicines, foods and cosmetics.
Description
Technical field
The present invention relates to the purposes of the anti-skin photoage of Fructus Arctii extract, belong to medicine, foods and cosmetics technical field.
Background technology
Health can be caused multiple danger by the ultraviolet in daylight.Ultraviolet (ultraviolet, UV) can be divided into short wave ultraviolet (UVC) 200-280nm, ultraviolet B radiation (UVB) 280-320nm, long wave ultraviolet (UVA) 320-400nm according to its wavelength.UVC is the strongest to the lethality of biological cell, but generally individually is filtered by Ozone in Atmosphere layer, and what arrive ground is mainly UVB and UVA.Inflammation is may occur in which through the skin of ultraviolet radiation, sunburn, aging, and the skin aging caused by the prolonged and repeated irradiation of ultraviolet, also known as photoaging.Change on histology is mainly, the change of dermal connective tissue, i.e. the deposition of the minimizing of collagen component and abnormal elastic fiber.Clinical manifestation is that skin wrinkle, lax rough, corse sweat pore, pigmentation, lentigo, telangiectasis etc. occurs, skin photoage not only causes damage capacitive performance, and can concurrent multiple dermatosis, such as seborrheic keratosis, solar lentigines keratinization etc., also having with cutaneous tumor and etiologic etiological contact such as common skin neoplasms such as scale cancer and base cancers, this all brings great puzzlement to the beautifying skin of people and health.
In recent years, along with the continuous destruction of atmospheric ozone layer, ozone layer is thinning gradually, and people contact ultraviolet chance and get more and more so that skin aging and skin carcinoma are continuously increased.Simultaneously along with the raising day by day of people's living standard, people are also more and more higher to the requirement of delay skin aging.Find and be prevented from ultraviolet radiation and cause the medicine of skin injury to become people instantly to pay special attention to and one of problem demanding prompt solution.The general toxic and side effects of natural active matter extracted from plant is little, safety is high, the acceptance of people is high, has certain research advantage.Fructus Arctii is the ripe dry fruit of feverfew Fructus Arctii (ArctiumlappaL.).The treatment of the diseases such as current Fructus Arctii is mainly used in anemopyretic cold clinically as China's Chinese medicine, acute sore throat swells and ache, measles without adequate eruption, carbuncle sore tumefacting virus, mumps sore throat.Simultaneously a lot of scientific research institution both domestic and external Fructus Arctii is carried out deeper into research, it was found that other pharmacological action multiple, such as antiviral, antiinflammatory, antitumor, anti-diabetic etc..But there is not yet the pertinent literature of Fructus Arctii extract's effect in photoprotection report so far.Be currently used for prevention, the product for the treatment of skin photoage is often accompanied with a large amount of side effect, and domestic and international Ge great cosmetics company and other research institutions are all actively researching and developing new product.The traditional Chinese medical science is the rarity of Chinese culture, is combined with the theory of modern life science by traditional Chinese medicine, is an effective way of exploitation Chinese herbal medicine, provides new strategy for new drug development.
Summary of the invention
Present invention solves the technical problem that the purposes being to provide the anti-skin photoage of a kind of Fructus Arctii extract.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
The purposes of the anti-skin photoage of Fructus Arctii extract.
Described Fructus Arctii extract purposes in the medicine preparing anti-skin photoage.
Described Fructus Arctii extract purposes in the cosmetics preparing anti-skin photoage.
Described Fructus Arctii extract purposes in the food preparing anti-skin photoage.
Described food includes health food.
Described Fructus Arctii extract is the ethanol extract of Fructus Arctii.
Described anti-skin photoage includes anti-UVA radiation and uv-resistant beta radiation.
The dosage form of described medicine includes Emulsion or unguentum.
Fructus Arctii (FructusArctii) is the dry mature fruit of Compositae biennial herb plant Fructus Arctii.Fructus Arctii extract in the present invention is the ethanol extract of Fructus Arctii.
The present invention studies by experiment and proposes Fructus Arctii extract first and can substantially resist the ultraviolet damaging action to skin.Tested by cellular level, present invention discover that Fructus Arctii extract can substantially reduce the ultraviolet damage to epidermis cell, epidermis cell is had protective effect.In zoopery, present invention discover that and embrocate Fructus Arctii extract's solution by skin surface, it is possible to substantially reduce the ultraviolet damage to mouse skin.Experimental result illustrates, the photoaging of skin is had protective effect by Fructus Arctii extract.The Fructus Arctii extract that the present invention proposes is applied to the novel medical use of prevention sun damage and photoaging, has the possibility used at human body skin further, has exploitation potential, can use by addition in everyday skincare.
Accompanying drawing explanation
Fig. 1 is that HaCaT cytoactive is affected schematic diagram by Fructus Arctii extract；
The survival rate of the HaCaT cell that UVB is irradiated by Tu2Shi Fructus Arctii extract affect schematic diagram；
Fig. 3 is HE colored light sem observation result figure (Normal group amplifies 100 times)；
Fig. 4 is HE colored light sem observation result figure (photoaging group amplifies 100 times)；
Fig. 5 is HE colored light sem observation result figure (low concentration Fructus Arctii protection group 1mg/ml amplifies 100 times)；
Fig. 6 is HE colored light sem observation result figure (middle concentration Fructus Arctii protection group 3mg/ml, amplify 100 times)；
Fig. 7 is HE colored light sem observation result figure (high concentration Fructus Arctii protection group 10mg/ml amplifies 100 times).
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is further described.
1. experiment material
Fructus Arctii extract is dissolved in DMSO solution, is made into the stock solution of 40mg/ml, is placed in-20 DEG C of preservations.It is diluted to desired concn with culture fluid during use.
2. cell is cultivated
Application on human skin horn cell (is purchased from Nanjing KaiJi Biology Science Development Co., Ltd), HaCaT cell is inoculated in DMEM culture medium (containing 10% hyclone, 100U/ml penicillin, 100 μ g/ml streptomycins), and culture bottle is positioned over 37 DEG C, 5%CO2Saturated humidity incubator is cultivated, within every 1-2 days, changes culture medium once.When Growth of Cells is to when covering bottle diapire 80%, with 0.25% trypsinization attached cell.
3. dosing and ultraviolet radiation process
Taking the logarithm the HaCaT cell of trophophase, after 2.5% trypsinization, counting, cell concentration is 1 × 104Individual, it is inoculated in 96 orifice plates with 100 μ L, if blank group (being left intact), matched group (be not added with Fructus Arctii extract and only accept ultra-vioket radiation) and experimental group (add and set concentration Fructus Arctii extract and accept ultra-vioket radiation).Next day, experimental group adds variable concentrations Fructus Arctii extract solution.After 48h, experimental group and matched group accept 120mj/cm2UVB irradiate.
4.MTT method detection cell survival rate
In 96 orifice plates, cell is accepting 120mj/cm2UVB irradiate after 24h, add MTT20 μ L/ hole, 37 DEG C hatch 4h after, abandon supernatant, every hole add DMSO150 μ l with dissolves first hairpin granule, gentle agitation dissolving.Optical density value (OD) is measured in wavelength 570nm when.
5. measure application on human skin horn cell SOD activity, MDA content
Each group cell accepts 120mj/cm224h after UVB irradiation, cell breakage processes, and extracts albumen, measures protein concentration.Operate requirement according to test kit, measure intracellular SOD and MDA content.
6. zoopery
Balb/C female mice (Shanghai Si Laike Company of Animals Ltd., cleaning grade), is randomly divided into 5 groups.With electric hair clipper, mouse back skin is shaved only, about 3*5cm, baring skin.
First group is Normal group, is left intact；
Second group is photoaging model group, and skin gives 180mj/cm every day2UVB irradiate.
Third and fourth, five groups be Fructus Arctii protection group, skin smears the Fructus Arctii solution of 10mg/ml, 3mg/ml, 1mg/ml every day respectively, gives 180mj/cm after 30min2UVB irradiate.
Irradiating and carry out once every day, totally 10 days, last experiment put to death mice after terminating.Take skin of back and carry out HE dyeing, measure MDA content, hydroxyproline content and SOD activity.
7. data statistic analysis
Experimental data is added and subtracted standard deviation _ X ± S with average and is represented, group difference compares employing t inspection.
Embodiment 1
Fructus Arctii extract's impact on HaCaT cytoactive:
After processing HaCaT cell 48h with the Fructus Arctii extract (200 μ g/ml, 100 μ g/ml, 50 μ g/ml, 25 μ g/ml, 12.5 μ g/ml) of variable concentrations, adopt mtt assay detection cell-proliferation activity.Result is as it is shown in figure 1, when Fructus Arctii extract's concentration is when 100 below μ g/ml, on the proliferation activity of HaCaT cell almost without producing any impact.In Fig. 1, dosing group compares with matched group,*P < 0.05,**P<0.01。
Embodiment 2
The protective effect of the HaCaT cell that UVB is irradiated by Fructus Arctii extract:
When with ultraviolet radiation cell, in cell, produce active oxygen, cell is caused damage, greatly reduces the proliferation activity of HaCaT cell.As in figure 2 it is shown, just there is notable protection HaCaT cell in Fructus Arctii extract from the UVB effect damaged when 25 μ g/ml, and this protective effect is dose dependent.In Fig. 2, dosing group compares with UVB irradiation group,*P < 0.05,**P<0.01。
Embodiment 3
The impact of SOD activity and MDA content in the HaCaT cell that UVB is irradiated by Fructus Arctii extract:
After UVB irradiation, the active oxygen produced in cell can destroy the activity of cell protein and enzyme, as made cell SOD activity reduce.Oxygen-derived free radicals attacks the polyunsaturated fatty acid on biomembrane, causes lipid peroxidation, forms lipid peroxidation product, and then causes cell injury.The content of MDA can reflect the snperoxiaized degree of body inner lipid and cell injury degree.As shown in table 1, the MDA content of UVB irradiation group is apparently higher than Normal group, and SOD activity is significantly lower than Normal group.And the HaCaT cell processed with Fructus Arctii extract at UVB pre-irradiation is compared with UVB irradiation group, SOD activity increases, MDA content reduces, and difference has statistical significance, illustrates that the oxidative damage that UVB irradiation is caused by Fructus Arctii extract has protective effect.
The impact (_ X ± S) of SOD activity and MDA content in the HaCaT cell that UVB is irradiated by table 1 Fructus Arctii extract
Compared with model group,*P < 0.05,**P<0.01。
Embodiment 4
Fructus Arctii extract's protective effect to the mouse skin that UVB irradiates:
The irradiation of UVB can be brought out skin and a series of photoaging damage such as wrinkling, paraplasm occurs.Fructus Arctii extract is configured to the solution of 10mg/ml, 3mg/ml, 1mg/ml by this example with deionized water, embrocates respectively and carries out 180mj/cm behind the back of Fructus Arctii protection group mice2UVB irradiate, after testing 10 days continuously, take mouse back skin and carry out HE colored light sem observation, as shown in Figure 3 and Figure 4, photoaging group is compared epidermis with Normal group and is substantially thickened, and the cell number of plies becomes many, and cell becomes big, inflammatory cell infiltration is shown in many, and fibroblast, skin appendages all have hypertrophy in various degree.As shown in Fig. 5,6,7, Fructus Arctii protection group relatively photoaging group pathological change has clear improvement, and corium group is thin compared with photoaging group, and inflammatory cell infiltration tails off.And it is obvious gradually with the increase of concentration to improve effect.
Ultraviolet radiation, particularly UVB can make skin produce too much reactive oxygen free radical (ROS), ROS inhibits skin self antioxidative defensive enginery, break the balance between oxidative and anti-oxidative, the cellular macromolecule materials such as DNA protein and lipid are attacked, forms response to oxidative stress.The anti-ROS enzyme that epidermal keratinocytes produces under normal circumstances, as superoxide dismutase (SOD), glutathion reductase (GSH-Px) etc. can resist certain ROS, but too much ROS can not be resisted, stop the generation of skin carcinoma.Take mouse back skin and carry out tissue homogenate, respectively MDA content, hydroxyproline content and SOD activity in detection skin histology.As shown in table 2, photoaging model group mice skin tissue MDA content substantially increases, and hydroxyproline content substantially reduces, and SOD activity substantially reduces.Fructus Arctii protection group compares with photoaging model group, and in mice skin tissue, MDA content substantially reduces, and hydroxyproline content substantially increases, and SOD activity substantially increases, and difference has statistical significance, illustrates that skin photoage is had protective action by Fructus Arctii extract.
Table 2 Fructus Arctii extract is on the impact (_ X ± S) of SOD, MDA, HYP in photoaging model mice skin
Compared with model group,*P < 0.05,**P<0.01；N=9.
The above is only the preferred embodiment of the present invention; it is noted that, for those skilled in the art; under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (8)
1. the purposes of the anti-skin photoage of Fructus Arctii extract.
2. purposes according to claim 1, it is characterised in that: described Fructus Arctii extract purposes in the medicine preparing anti-skin photoage.
3. purposes according to claim 1, it is characterised in that: described Fructus Arctii extract purposes in the cosmetics preparing anti-skin photoage.
4. purposes according to claim 1, it is characterised in that: described Fructus Arctii extract purposes in the food preparing anti-skin photoage.
5. purposes according to claim 4, it is characterised in that: described food includes health food.
6. the purposes according to any one of claim 1 ~ 5, it is characterised in that: described Fructus Arctii extract is the ethanol extract of Fructus Arctii.
7. the purposes according to any one of claim 1 ~ 5, it is characterised in that: described anti-skin photoage includes anti-UVA radiation and uv-resistant beta radiation.
8. purposes according to claim 2, it is characterised in that: the dosage form of described medicine includes Emulsion or unguentum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610189214.7A CN105769964A (en) | 2016-03-30 | 2016-03-30 | Application of fructus arctii extract in resistance of skin photoage |
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| CN201610189214.7A CN105769964A (en) | 2016-03-30 | 2016-03-30 | Application of fructus arctii extract in resistance of skin photoage |
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| CN201610189214.7A Pending CN105769964A (en) | 2016-03-30 | 2016-03-30 | Application of fructus arctii extract in resistance of skin photoage |
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| CN107951912A (en) * | 2016-10-17 | 2018-04-24 | 贝安股份有限公司 | Burdock extract is used to prepare the purposes of protection elastomer and collagen medicine |
| JP2018076237A (en) * | 2016-11-07 | 2018-05-17 | 貝安股▲ふん▼有限公司 | Application of drug which protects elastic fibers and collagen which were produced using arctium lappa extract |
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Address after: 215123 Suzhou Industrial Park, Jiangsu Province, if the water No. 388 D505, D507 room Applicant after: Qin Hao Pharmaceutical (Suzhou) Co., Ltd. Address before: 215123 Suzhou Industrial Park, Jiangsu Province, if the waterway No. 388 D507 Applicant before: SUZHOU GENHOUSE PHARMACEUTICAL RESEARCH AND DEVELOPMENT CO., LTD. |
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