CN112472638B - Anti-aging matrix and preparation method and application thereof - Google Patents
Anti-aging matrix and preparation method and application thereof Download PDFInfo
- Publication number
- CN112472638B CN112472638B CN201910957516.8A CN201910957516A CN112472638B CN 112472638 B CN112472638 B CN 112472638B CN 201910957516 A CN201910957516 A CN 201910957516A CN 112472638 B CN112472638 B CN 112472638B
- Authority
- CN
- China
- Prior art keywords
- goldenrain
- aging
- solution
- tree fruit
- palmitoyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an anti-aging matrix which is prepared from the following components in percentage by weight: 3-5% of 1, 2-pentanediol, 810-20% of acetyl hexapeptide, 15-7% of palmitoyl tripeptide, 75-8% of palmitoyl tetrapeptide, 21-5% of acetyl tetrapeptide, 53-6% of palmitoyl tripeptide, 0.01-0.1% of pepper seed extract, 10-20% of butanediol, 5-10% of propylene glycol, 1-5% of inositol, 0.05-0.1% of goldenrain fruit extract and the balance of deionized water. The invention also provides a preparation method and application of the anti-aging matrix. The anti-aging matrix provided by the invention has a good anti-aging effect, and the anti-aging effect of the skin care product can be remarkably improved by adding the anti-aging matrix into the skin care products such as astringent, emulsion, cream and the like.
Description
Technical Field
The invention relates to an anti-aging matrix and a preparation method and application thereof.
Background
The skin care product is a product for protecting skin, and is a complex mixture processed by various raw materials through a formula. There are many skin care products, and the skin care products can be roughly classified into: nourishing and moisturizing, anti-wrinkle and anti-aging, freckle removing and whitening, sun screening, slimming, depilation, and the like. In the formula of cosmetics, the cosmetic is generally divided into a base material and an auxiliary material. The base is a substance constituting the main body of the cosmetic, and is a substance having a main role in the cosmetic. The auxiliary materials have the functions of forming, stabilizing or endowing color, fragrance and characteristics of cosmetics, and the consumption of the auxiliary raw materials in the formula of the cosmetics is not large but is important.
Chinese patent application CN201510178149.3 discloses a cleaning and skin-care matrix and a preparation method thereof, wherein the cleaning and skin-care matrix comprises the following components in percentage by mass: 8-13% of lauric acid, 6-10% of stearic acid, 6-10% of palmitic acid, 3-6% of myristic acid, 1-4% of a first emulsifier and 1-3% of a second emulsifier; 10-15% of a first humectant, 1-3% of a pH regulator, 6-10.5% of a second humectant, 1.25-2.35% of a first thickener and 0.02-0.15% of a chelating agent; 6-11% of a first surfactant, 1.0-3.25% of a second surfactant, 0.85-2.25% of a third surfactant and 1.02-2.35% of a fourth surfactant; 0.82-1.54% of a second thickening agent; the balance being water. The present invention has a problem that it has substantially no anti-aging effect.
Disclosure of Invention
The technical problem to be solved by the invention is to provide an anti-aging matrix which has a good anti-aging effect, and the anti-aging effect of a skin care product can be remarkably improved by adding the anti-aging matrix into the skin care products such as astringent, emulsion, cream and the like.
In order to solve the technical problems, the technical scheme of the invention is as follows:
an anti-aging matrix is prepared from the following components in percentage by weight: 3-5% of 1, 2-pentanediol, 810-20% of acetyl hexapeptide, 15-7% of palmitoyl tripeptide, 75-8% of palmitoyl tetrapeptide, 21-5% of acetyl tetrapeptide, 53-6% of palmitoyl tripeptide, 0.01-0.1% of pepper seed extract, 10-20% of butanediol, 5-10% of propylene glycol, 1-5% of inositol, 0.05-0.1% of goldenrain fruit extract and the balance of deionized water.
Further, the invention is prepared from the following components in percentage by weight: 4% of 1, 2-pentanediol, 816% of acetyl hexapeptide, 16% of palmitoyl tripeptide, 76% of palmitoyl tetrapeptide, 24% of acetyl tetrapeptide, 55% of palmitoyl tripeptide, 0.04% of pepper seed extract, 20% of butanediol, 10% of propylene glycol, 3% of inositol, 0.06% of goldenrain fruit extract and the balance of water.
Further, the preparation method of the goldenrain tree fruit extract comprises the following steps:
removing peel and core of goldenrain tree fruit to obtain goldenrain tree fruit pulp, freeze-drying goldenrain tree fruit pulp for 4 hours, crushing the goldenrain tree fruit pulp, sieving the goldenrain tree fruit pulp with a 40-mesh sieve to obtain goldenrain tree fruit pulp powder, adding the goldenrain tree fruit pulp powder into an ethanol aqueous solution, carrying out ultrasonic extraction for 20 minutes to obtain an extracting solution, centrifuging the extracting solution for 20 minutes to obtain a supernatant, and freeze-drying the supernatant to constant weight to obtain the goldenrain tree fruit extract.
Further, in the preparation steps of the goldenrain tree fruit extract, the volume fraction of the ethanol aqueous solution is 80%, the ratio of the goldenrain tree fruit meat meal to the ethanol aqueous solution is 1:40g/mL, the frequency of ultrasonic extraction is 500Hz, and the speed of centrifugation is 8000 rpm.
The invention also provides a preparation method of the anti-aging matrix.
In order to solve the technical problems, the technical scheme is as follows:
a method of preparing an anti-aging matrix comprising the steps of:
s1, mixing a pepper seed extract, propylene glycol and a goldenrain fruit extract, heating to 50-55 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing and stirring the palmitoyl tripeptide-5, the butanediol and the solution I for 1-5 minutes to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 84-86 ℃, preserving heat for 30 minutes, and then cooling to 44-46 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring for 5-10 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
Further, the stirring speed in the step S2 is 100-200 r/min; the stirring speed in the step S4 is 800-1000 rpm.
The invention also provides application of the anti-aging matrix in preparation of skin care products.
Further, the anti-aging matrix accounts for 10-40% of the skin care product by weight.
Further, the skin care product comprises the following components in percentage by weight: 10-40% of anti-aging matrix, 0.18-0.2% of triethanolamine, 6.0-8.0% of glycerol, 1-5% of 1, 2-pentanediol, 0.18-0.2% of carbomer and the balance of deionized water.
Compared with the prior art, the invention has the following beneficial effects:
(1) the components which can be degraded and transdermally absorbed by skin and can supply nutrition to skin are reasonably screened out, and through reasonable compatibility, the components can generate a synergistic interaction effect, so that the prepared matrix has a good anti-aging effect, and the anti-aging effect of the cosmetics can be remarkably improved by adding the anti-aging matrix into skin care product formulas such as astringent, emulsion, cream and the like.
(2) The pepper seed extract used in the invention has better anti-aging effect, better anti-oxidation and antibacterial activity and dispersibility, and thus can effectively improve the anti-oxidation and antibacterial effects of the anti-aging matrix.
(3) The goldenrain tree fruit extract which is prepared by using goldenrain tree koelreuteria paniculata as a raw material and using an ethanol water solution as an extraction solvent through ultrasonic-assisted extraction is also added, and has good antioxidant activity, so that the antioxidant effect of the anti-aging matrix can be further improved, and on the other hand, the goldenrain tree fruit extract can also improve the blackhead removing effect and stability of the anti-aging matrix.
Detailed Description
The present invention will be described in detail with reference to specific embodiments, and the exemplary embodiments and descriptions thereof herein are provided to explain the present invention but not to limit the present invention.
Example 1
The anti-aging matrix is prepared from the following components in percentage by weight: 4% of 1, 2-pentanediol, 816% of acetyl hexapeptide, 16% of palmitoyl tripeptide, 76% of palmitoyl tetrapeptide, 24% of acetyl tetrapeptide, 55% of palmitoyl tripeptide, 0.04% of pepper seed extract, 20% of butanediol, 10% of propylene glycol, 3% of inositol, 0.06% of goldenrain fruit extract and the balance of water. The preparation method of the goldenrain tree fruit extract comprises the following steps:
removing peel and kernel of goldenrain tree fruit to obtain goldenrain tree fruit pulp, freeze-drying goldenrain tree fruit pulp for 4 hours, crushing the goldenrain tree fruit pulp, sieving the goldenrain tree fruit pulp with a 40-mesh sieve to obtain goldenrain tree fruit pulp powder, adding the goldenrain tree fruit pulp powder into an ethanol aqueous solution with the volume fraction of 80%, ultrasonically extracting the goldenrain tree fruit pulp powder and the ethanol aqueous solution for 20 minutes under the condition of 500Hz to obtain an extracting solution, centrifuging the extracting solution for 20 minutes at the speed of 8000 rpm to obtain a supernatant, and freeze-drying the supernatant to constant weight to obtain the goldenrain tree fruit extract.
The preparation method of this example includes the following steps:
s1, mixing a pepper seed extract, propylene glycol and a goldenrain fruit extract, heating to 54 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing the palmitoyl tripeptide-5, butanediol and the solution I, and stirring for 3 minutes at a stirring speed of 150 revolutions per minute to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 85 ℃, keeping the temperature for 30 minutes, and then cooling to 45 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring at the stirring speed of 900 rpm for 8 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
Example 1.1
The skin care product comprises the following components in percentage by weight: the anti-aging matrix prepared in example 1 comprises 10% of triethanolamine, 0.19% of glycerol, 4% of 1, 2-pentanediol, 0.19% of carbomer and the balance of deionized water.
Example 1.2
The skin care product comprises the following components in percentage by weight: 20% of the anti-aging matrix prepared in example 1, 0.19% of triethanolamine, 7% of glycerol, 4% of 1, 2-pentanediol, 0.19% of carbomer and the balance deionized water.
Example 1.3
The skin care product comprises the following components in percentage by weight: 30% of the anti-aging substrate prepared in example 1, 0.19% of triethanolamine, 7% of glycerol, 4% of 1, 2-pentanediol, 0.19% of carbomer, and the balance of deionized water.
Example 1.4
The skin care product comprises the following components in percentage by weight: 40% of the anti-aging matrix prepared in example 1, 0.19% of triethanolamine, 7% of glycerol, 4% of 1, 2-pentanediol, 0.19% of carbomer and the balance deionized water.
Comparative example: example 1 of patent application No. CN 201510178149.3.
The first experimental example: anti-aging efficacy test
The test method comprises the following steps: 6 test subjects with age of 25-35 years and more wrinkles are selected, and 6 test subjects are respectively coated on wrinkles every morning and are provided with the test samples of example 1, example 1.1-1.4 and the comparative example once a week, and the wrinkle area reduction rate, the wrinkle number reduction rate and the wrinkle depth reduction rate are recorded, wherein the wrinkle area reduction rate is (wrinkle area before test-wrinkle area after test)/wrinkle area before test × 100%, the wrinkle number reduction rate is (wrinkle number before test-wrinkle number after test)/wrinkle area before test × 100%, the wrinkle depth reduction rate is (wrinkle depth before test-wrinkle depth after test)/wrinkle depth before test × 100%, the wrinkle area reduction rate, the wrinkle number reduction rate and the wrinkle depth reduction rate are larger, and the anti-aging effect is better. The test results are shown in table 1:
| wrinkle area reduction ratio (%) | Wrinkle number reduction ratio (%) | Wrinkle depth reduction Rate (%) | |
| Example 1 | 50.3％ | 48.2％ | 55.6％ |
| Example 1.1 | 27.3％ | 27.1％ | 26.2％ |
| Example 1.2 | 32.1％ | 33.2％ | 34.5％ |
| Example 1.3 | 42.1％ | 40.7％ | 43.2％ |
| Example 1.4 | 44.1％ | 43.3％ | 45.1％ |
| Comparative example | 3.2％ | 2.8％ | 2.6％ |
TABLE 1
Table 1 shows that the wrinkle area reduction rate, the wrinkle number reduction rate, and the wrinkle depth reduction rate of examples 1 and 1.1 to 1.4 of the present invention are significantly greater than those of the comparative examples, indicating that the present invention has a better anti-aging effect, and the greater the proportion of the anti-aging base in the skin care product, the better the anti-aging effect of the skin care product.
Example 2
The anti-aging matrix is prepared from the following components in percentage by weight: 3% of 1, 2-pentanediol, 820% of acetyl hexapeptide, 16.5% of palmitoyl tripeptide, 77% of palmitoyl tetrapeptide, 21% of acetyl tetrapeptide, 56% of palmitoyl tripeptide, 0.08% of pepper seed extract, 10% of butanediol, 6% of propylene glycol, 5% of inositol, 0.05% of goldenrain fruit extract and the balance of water. The procedure for the preparation of goldenrain fruit extract was the same as in example 1.
The preparation method of this example includes the following steps:
s1, mixing a pepper seed extract, propylene glycol and a goldenrain fruit extract, heating to 55 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing the palmitoyl tripeptide-5, butanediol and the solution I, and stirring for 1 minute at a stirring speed of 120 revolutions per minute to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 85 ℃, keeping the temperature for 30 minutes, and then cooling to 45 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring at the stirring speed of 1000 rpm for 5 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
Example 3
The anti-aging matrix is prepared from the following components in percentage by weight: 5% of 1, 2-pentanediol, 810% of acetyl hexapeptide, 15% of palmitoyl tripeptide, 78% of palmitoyl tetrapeptide, 22% of acetyl tetrapeptide, 54% of palmitoyl tripeptide, 0.01% of pepper seed extract, 15% of butanediol, 5% of propylene glycol, 1% of inositol, 0.1% of goldenrain fruit extract and the balance of water. The procedure for the preparation of goldenrain fruit extract was the same as in example 1.
The preparation method of this example includes the following steps:
s1, mixing a pepper seed extract, propylene glycol and a goldenrain fruit extract, heating to 50 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing the palmitoyl tripeptide-5, butanediol and the solution I, and stirring for 5 minutes at a stirring speed of 100 revolutions per minute to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 86 ℃, preserving heat for 30 minutes, and then cooling to 46 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring at the stirring speed of 800 rpm for 10 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
Example 4
The anti-aging matrix is prepared from the following components in percentage by weight: 4.5% of 1, 2-pentanediol, acetyl hexapeptide-818%, palmitoyl tripeptide-17%, palmitoyl tetrapeptide-75%, acetyl tetrapeptide-25%, palmitoyl tripeptide-53%, 0.1% of pepper seed extract, 18% of butanediol, 9% of propylene glycol, 4% of inositol, 0.07% of goldenrain fruit extract and the balance of water. The procedure for the preparation of goldenrain fruit extract was the same as in example 1.
The preparation method of this example includes the following steps:
s1, mixing pepper seed extract, propylene glycol and goldenrain fruit extract, heating to 52 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing the palmitoyl tripeptide-5, butanediol and the solution I, and stirring for 4 minutes at a stirring speed of 200 revolutions per minute to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 84 ℃, preserving heat for 30 minutes, and then cooling to 44 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring at the stirring speed of 850 rpm for 9 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
Example 5
The skin care product comprises the following components in percentage by weight: the anti-aging matrix prepared in example 2 comprises 20% of triethanolamine, 0.18% of glycerol, 3% of 1, 2-pentanediol, 0.19% of carbomer and the balance of deionized water.
Example 6
The skin care product comprises the following components in percentage by weight: 30% of the anti-aging substrate prepared in example 3, 0.2% of triethanolamine, 6% of glycerol, 1, 2-pentanediol, 0.2% of carbomer, and the balance of deionized water.
Example 7
The skin care product comprises the following components in percentage by weight: 40% of the anti-aging substrate prepared in example 4, 0.19% of triethanolamine, 7% of glycerol, 5% of 1, 2-pentanediol, 0.18% of carbomer, and the balance deionized water.
Reference example 1
In contrast to example 1, the anti-aging matrix was not comprised of pepper seed extract.
Reference example 2
In contrast to example 1, the anti-aging base does not comprise an extract of goldenrain tree fruit in its composition.
Experiment example two: test for antioxidant efficacy
DPPH radical scavenging rates of examples 1-4, reference examples 1-2, and comparative examples were measured by DPPH radical scavenging experiments, respectively, and a larger DPPH radical scavenging rate indicates a better antioxidant effect. The test results are shown in table 2:
TABLE 2
Table 2 shows that the DPPH free radical clearance rates of the invention examples 1-4 are all significantly greater than the comparative examples, indicating that the invention has better antioxidant efficacy. The partial compositions of reference examples 1 and 2 are different from example 1, and the DPPH free radical clearance rate of reference examples 1 and 2 is reduced to different degrees, which shows that the pepper seed extract and the goldenrain tree fruit extract can improve the antioxidant effect.
Experiment example three: antimicrobial efficacy testing
The diameters of the inhibition zones of examples 1-4, reference examples 1-2 and comparative examples on staphylococcus aureus, escherichia coli and candida albicans are respectively determined through inhibition zone experiments, the larger the diameter of the inhibition zone is, the better the antibacterial effect is, and the test results are shown in table 3:
TABLE 3
Table 3 shows that the diameters of inhibition zones of examples 1 to 4 of the invention on staphylococcus aureus, escherichia coli and candida albicans are obviously larger than those of comparative examples, which indicates that the invention has better antibacterial efficacy. The components of reference examples 1 and 2 are different from those of reference example 1, and the diameters of inhibition zones of reference example 1 on staphylococcus aureus, escherichia coli and candida albicans are greatly reduced, which indicates that the pepper seed extract is the key for improving the antibacterial efficacy.
Experimental example four: blackhead removal efficacy test
The test method comprises the following steps: selecting 84 testers with the ages of 20-32 and more blackheads on the face, randomly and averagely dividing the testers into 7 groups, respectively using the embodiment 1-4, the reference embodiment 1-2 and the comparative example for cleaning the skin for 1 time in the morning and evening for each group of testers, grading the blackhead removing effect by the testers after one week by adopting a 5-point full-scale grading system, wherein 1 point shows that the effect is poor, 2 points shows that the effect is poor, 3 points shows that the effect is general, 4 points shows that the effect is better, 5 points shows that the effect is very good, and calculating the average blackhead removing effect score of each group of testers.
The test results are shown in table 4:
| average effect of removing blackheads | |
| Example 1 | 4.67 |
| Example 2 | 4.58 |
| Example 3 | 4.58 |
| Example 4 | 4.5 |
| Reference example 1 | 4.58 |
| Reference example 2 | 2.08 |
| Comparative example | 1.67 |
TABLE 4
Table 4 shows that the average black head removing effect of the examples 1 to 4 of the invention is obviously greater than that of the comparative example, which shows that the invention has better black head removing effect. The partial components of reference examples 1 and 2 are different from those of example 1, and the average reduction of the black heads of reference example 2 is much, which shows that the goldenrain fruit extract is the key for improving the antibacterial efficacy.
Experimental example five: stability test
Heat resistance stability test method: examples 1 to 4 and reference examples 1 to 2 were placed in an environment of 40 ℃ and maintained for 24 hours, and then returned to room temperature, and the presence or absence of turbidity was observed.
The cold resistance stability test method comprises the following steps: the samples of examples 1 to 4 and reference examples 1 to 2 were placed in an environment of-10 ℃ for 24 hours, and then returned to room temperature, and the presence or absence of the delamination and discoloration was observed.
The test results are shown in table 5:
| | | stability against Heat | Stability against cold |
| Example 1 | No turbidity phenomenon | No layered discoloration |
| Example 2 | No turbidity phenomenon | No layered discoloration |
| Example 3 | No turbidity phenomenon | No layered discoloration |
| Example 4 | No turbidity phenomenon | No layered discoloration |
| Reference example 1 | No turbidity phenomenon | No layered discoloration |
| Reference example 2 | Turbidity appears | The phenomenon of layered color change occurs |
TABLE 5
Table 5 shows that the samples 1 to 4 of the invention have no turbidity through a heat resistance test and no layered discoloration through a cold resistance test, which indicates that the invention has better stability. The compositions of reference examples 1-2 are different from those of example 1, and reference example 2 shows clouding phenomenon through heat resistance test and layered discoloration phenomenon through cold resistance test, indicating that goldenrain tree fruit extract is the key to improve stability.
The above-described embodiments are merely illustrative of the principles and capabilities of the present invention and are not intended to be limiting. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and scope of the present invention as defined in the appended claims.
Claims (7)
1. An anti-aging matrix characterized by: the composition is prepared from the following components in percentage by weight: 3-5% of 1, 2-pentanediol, 810-20% of acetyl hexapeptide, 15-7% of palmitoyl tripeptide, 75-8% of palmitoyl tetrapeptide, 21-5% of acetyl tetrapeptide, 53-6% of palmitoyl tripeptide, 0.01-0.1% of pepper seed extract, 10-20% of butanediol, 5-10% of propylene glycol, 1-5% of inositol, 0.05-0.1% of goldenrain fruit extract and the balance of deionized water;
the preparation method of the goldenrain tree fruit extract comprises the following steps: removing peel and core of goldenrain tree fruit to obtain goldenrain tree fruit pulp, freeze-drying goldenrain tree fruit pulp for 4 hours, crushing the goldenrain tree fruit pulp, sieving the goldenrain tree fruit pulp with a 40-mesh sieve to obtain goldenrain tree fruit pulp powder, adding the goldenrain tree fruit pulp powder into an ethanol aqueous solution, carrying out ultrasonic extraction for 20 minutes to obtain an extracting solution, centrifuging the extracting solution for 20 minutes to obtain a supernatant, and freeze-drying the supernatant to constant weight to obtain a goldenrain tree fruit extract;
in the preparation steps of the goldenrain tree fruit extract, the volume fraction of an ethanol aqueous solution is 80%, the ratio of goldenrain tree fruit meat powder to the ethanol aqueous solution is 1:40g/mL, the frequency of ultrasonic extraction is 500Hz, and the centrifugation speed is 8000 rpm.
2. The anti-aging matrix according to claim 1, wherein: the composition is prepared from the following components in percentage by weight: 4% of 1, 2-pentanediol, 816% of acetyl hexapeptide, 16% of palmitoyl tripeptide, 76% of palmitoyl tetrapeptide, 24% of acetyl tetrapeptide, 55% of palmitoyl tripeptide, 0.04% of pepper seed extract, 20% of butanediol, 10% of propylene glycol, 3% of inositol, 0.06% of goldenrain fruit extract and the balance of deionized water.
3. The method for preparing an anti-aging matrix according to any one of claims 1 to 2, wherein the method comprises the following steps: the method comprises the following steps:
s1, mixing a pepper seed extract, propylene glycol and a goldenrain fruit extract, heating to 50-55 ℃, and stirring until the pepper seed extract, the propylene glycol and the goldenrain fruit extract are completely dissolved to obtain a solution I;
s2, mixing and stirring the palmitoyl tripeptide-5, the butanediol and the solution I for 1-5 minutes to obtain a solution II;
s3, mixing deionized water, 1, 2-pentanediol, inositol and the solution II, heating to 84-86 ℃, preserving heat for 30 minutes, and then cooling to 44-46 ℃ to obtain a transparent solution;
s4, adding acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 into the transparent solution, stirring for 5-10 minutes, and filtering to remove impurities to obtain the anti-aging matrix.
4. The method of preparing an anti-aging matrix according to claim 3, wherein: the stirring speed in the step S2 is 100-200 r/min; the stirring speed in the step S4 is 800-1000 rpm.
5. Use of the anti-aging substrate according to any one of claims 1 to 2 or the anti-aging substrate prepared by the method for preparing an anti-aging substrate according to any one of claims 3 to 4 for the preparation of a skin care product.
6. Use according to claim 5, characterized in that: the weight percentage of the anti-aging matrix in the skin care product is 10-40%.
7. Use according to claim 6, characterized in that: the skin care product comprises the following components in percentage by weight: 10-40% of anti-aging matrix, 0.18-0.2% of triethanolamine, 6.0-8.0% of glycerol, 1-5% of 1, 2-pentanediol, 0.18-0.2% of carbomer and the balance of deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910957516.8A CN112472638B (en) | 2019-10-10 | 2019-10-10 | Anti-aging matrix and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910957516.8A CN112472638B (en) | 2019-10-10 | 2019-10-10 | Anti-aging matrix and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112472638A CN112472638A (en) | 2021-03-12 |
| CN112472638B true CN112472638B (en) | 2022-05-31 |
Family
ID=74920902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910957516.8A Active CN112472638B (en) | 2019-10-10 | 2019-10-10 | Anti-aging matrix and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112472638B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113491657A (en) * | 2021-07-12 | 2021-10-12 | 广东丸美生物技术股份有限公司 | Cosmetic matrix with anti-aging effect and preparation method and application thereof |
| CN115844751B (en) * | 2022-11-23 | 2024-07-12 | 上海新高姿化妆品有限公司 | Synergistic anti-aging peptide composition and application thereof |
| CN117547478B (en) * | 2024-01-12 | 2024-03-26 | 珠海金肽生物科技有限公司 | Anti-saccharification and anti-aging composition and application thereof in preparation of skin care products |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380298A (en) * | 2002-04-26 | 2002-11-20 | 林文翰 | Method for separating and extracting total flavone from goldenrain tree plant and its application |
| CN106726668A (en) * | 2016-11-19 | 2017-05-31 | 诺斯贝尔化妆品股份有限公司 | A kind of many peptidoliposome raw materials used in cosmetics |
| CN108703894A (en) * | 2018-08-21 | 2018-10-26 | 广东博然堂生物科技有限公司 | It is a kind of that there is whitening, anti-ageing complex polypeptide composition and preparation method thereof |
| CN108904320A (en) * | 2018-09-20 | 2018-11-30 | 深圳市维琪医药研发有限公司 | A kind of peptide composition of prevention and improvement striae of pregnancy |
| CN110151597A (en) * | 2019-05-14 | 2019-08-23 | 夏战利 | A kind of polypeptide repair latex and preparation method thereof |
| CN110269814A (en) * | 2019-03-19 | 2019-09-24 | 广州市拉凯尔干细胞研究所 | A kind of preparation method of the crease-resistant composite skin care product containing Argireline |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265348B (en) * | 2016-09-19 | 2020-05-19 | 广东丸美生物技术股份有限公司 | Substrate with skin barrier repairing and anti-aging effects and preparation method and application thereof |
| CN108420749A (en) * | 2018-05-11 | 2018-08-21 | 广东省中药研究所 | A kind of skin care item and preparation method thereof with anti-aging effects |
| CN109172491A (en) * | 2018-11-22 | 2019-01-11 | 赛杜恳医药生物科技（上海）有限公司 | A kind of creme and preparation method thereof with anti-aging and activating microcirculation and removing stasis medicinal function |
| CN109464342B (en) * | 2018-12-29 | 2019-11-08 | 广州市盛美化妆品有限公司 | A kind of anti-aging Essence and preparation method thereof |
-
2019
- 2019-10-10 CN CN201910957516.8A patent/CN112472638B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380298A (en) * | 2002-04-26 | 2002-11-20 | 林文翰 | Method for separating and extracting total flavone from goldenrain tree plant and its application |
| CN106726668A (en) * | 2016-11-19 | 2017-05-31 | 诺斯贝尔化妆品股份有限公司 | A kind of many peptidoliposome raw materials used in cosmetics |
| CN108703894A (en) * | 2018-08-21 | 2018-10-26 | 广东博然堂生物科技有限公司 | It is a kind of that there is whitening, anti-ageing complex polypeptide composition and preparation method thereof |
| CN108904320A (en) * | 2018-09-20 | 2018-11-30 | 深圳市维琪医药研发有限公司 | A kind of peptide composition of prevention and improvement striae of pregnancy |
| CN110269814A (en) * | 2019-03-19 | 2019-09-24 | 广州市拉凯尔干细胞研究所 | A kind of preparation method of the crease-resistant composite skin care product containing Argireline |
| CN110151597A (en) * | 2019-05-14 | 2019-08-23 | 夏战利 | A kind of polypeptide repair latex and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| "胡椒里面的子能不能吃;于莎莎;《百度》;20141005;第1页 于莎莎健康指导 * |
| 栾树不同部位总黄酮的提取及含量测定;唐纯翼等;《中国农学通报》;20051030;第21卷(第05期);第159--163页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112472638A (en) | 2021-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112472638B (en) | Anti-aging matrix and preparation method and application thereof | |
| CN105395458B (en) | Herbal composition and application thereof | |
| JP2018203628A (en) | Cosmetic composition or food and drink composition | |
| WO2011036786A1 (en) | Stress-alleviating agent comprising plant-derived polyamine-containing extract as active ingredient | |
| JP2000229827A (en) | Skin lotion | |
| JP2017052706A (en) | Cosmetic composition containing granules | |
| JPWO2007148737A1 (en) | Method for preparing plant extract, plant extract and use thereof | |
| JP6016878B2 (en) | Proteoglycan production method | |
| JP7083818B2 (en) | Placenta extract | |
| JP2007204423A (en) | Method for producing extract of bamboo grass and use of the extract | |
| CN111346053B (en) | Natural emulsifier with biological activity and application thereof | |
| JPH10158305A (en) | Antimicrobial and antiseptic agent containing chitosan derivative | |
| JP2001220340A (en) | Skin preparation for external use for preventing aging | |
| TWI801655B (en) | Composition for improving skin transparency and dullness | |
| JP2013166785A (en) | Stress-reducing agent of plant origin | |
| JP2002145730A (en) | Cosmetic | |
| JP2002284633A (en) | Cosmetics | |
| JP2000226332A (en) | Anti-mutagenically active substance | |
| RU2500380C1 (en) | COSMETIC PRODUCT Dea vita FOR FACE AND BODY SKIN CARE | |
| CN105106101A (en) | Whitening and anti-wrinkling sun cream and preparation method thereof | |
| KR101801063B1 (en) | The method of aqua cosmetic cream with ginseng extract | |
| JP2003171258A (en) | Cosmetic composition | |
| JP2001247442A (en) | Skin preparation for externaluse | |
| JP2003128698A (en) | Acylated attelo collagen from fish skin, its manufacturing method, and fine cosmetic compounded with its compound | |
| KR102520908B1 (en) | Preparation of aged solution of fruit of dioscorea opposita thumb, and aged solution of fruit of dioscorea opposita thumb prepared thereby |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |