CN112470004A - 用于组织表征和筛查的增强型细胞计量术 - Google Patents

用于组织表征和筛查的增强型细胞计量术 Download PDF

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Publication number
CN112470004A
CN112470004A CN201980030877.6A CN201980030877A CN112470004A CN 112470004 A CN112470004 A CN 112470004A CN 201980030877 A CN201980030877 A CN 201980030877A CN 112470004 A CN112470004 A CN 112470004A
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China
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sample
lens arrangement
platen
light
channel
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Pending
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CN201980030877.6A
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English (en)
Chinese (zh)
Inventor
N·阿维恩丹
S·麦希
欧瓘青
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Wu Rui Treatment Co ltd
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Wu Rui Treatment Co ltd
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Publication of CN112470004A publication Critical patent/CN112470004A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/50—Mixing liquids with solids
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B13/00—Optical objectives specially designed for the purposes specified below
    • G02B13/22—Telecentric objectives or lens systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B15/00—Optical objectives with means for varying the magnification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0088—Inverse microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/26—Stages; Adjusting means therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00418—Means for dispensing and evacuation of reagents using pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201980030877.6A 2018-05-25 2019-05-23 用于组织表征和筛查的增强型细胞计量术 Pending CN112470004A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862676620P 2018-05-25 2018-05-25
US62/676,620 2018-05-25
PCT/US2019/033755 WO2019226897A2 (en) 2018-05-25 2019-05-23 Enhanced cytometry for tissue characterization and screening

Publications (1)

Publication Number Publication Date
CN112470004A true CN112470004A (zh) 2021-03-09

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980030877.6A Pending CN112470004A (zh) 2018-05-25 2019-05-23 用于组织表征和筛查的增强型细胞计量术

Country Status (5)

Country Link
US (2) US12235264B2 (OSRAM)
EP (1) EP3803397A2 (OSRAM)
JP (2) JP7489326B2 (OSRAM)
CN (1) CN112470004A (OSRAM)
WO (1) WO2019226897A2 (OSRAM)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11346763B2 (en) 2019-12-18 2022-05-31 Jiangnan University Apparatus and method for microbial cell counting
CN110846217B (zh) * 2019-12-18 2024-07-05 江南大学 用于微生物细胞计数的仪器及方法
EP4337933A4 (en) 2021-05-14 2024-10-23 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS BY SPECTRAL DISCRIMINATION AND METHODS OF USE THEREOF
WO2026008821A1 (en) * 2024-07-05 2026-01-08 Miltenyi Biotec B.V. & Co. KG Method for continuous single molecule localization of conventional fluorescent antibody probes

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20100019157A1 (en) * 2008-07-25 2010-01-28 Roche Molecular Systems, Inc. Excitation and Imaging Optics for Fluorescence Detection
US20150323462A1 (en) * 2014-05-12 2015-11-12 Cellomics, Inc. Automated imaging of chromophore labeled samples
US20160216503A1 (en) * 2015-01-26 2016-07-28 California Institute Of Technology Array level fourier ptychographic imaging

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CN101419170A (zh) * 1996-08-16 2009-04-29 Ge保健尼亚加拉公司 用于分析井板、凝胶和斑点的数字成像系统
WO2000068671A2 (en) 1999-05-12 2000-11-16 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
US6867851B2 (en) * 1999-11-04 2005-03-15 Regents Of The University Of Minnesota Scanning of biological samples
JP4199609B2 (ja) 2002-07-12 2008-12-17 三菱化学株式会社 分析用チップ、分析用チップユニット及び分析装置ならびに分析用チップの作製方法
WO2005031322A1 (ja) 2003-09-26 2005-04-07 Nikon Corporation 環境保持装置および環境制御型分析装置
JP4864519B2 (ja) 2006-04-11 2012-02-01 浜松ホトニクス株式会社 光測定装置、光測定方法、及び光測定プログラム
JP5013410B2 (ja) 2007-03-14 2012-08-29 独立行政法人産業技術総合研究所 微粒子測定方法及び装置
JP2009015047A (ja) 2007-07-05 2009-01-22 Nikon Corp 顕微鏡装置、及び観察方法
JP2013150567A (ja) 2012-01-25 2013-08-08 Hitachi High-Technologies Corp 核酸分析用反応デバイス、及び核酸分析装置
JP2014081321A (ja) 2012-10-18 2014-05-08 Fujikura Ltd 流路デバイス及び分析方法
JP2017049069A (ja) 2015-08-31 2017-03-09 国立研究開発法人理化学研究所 撮像装置、及び、生体情報取得装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100019157A1 (en) * 2008-07-25 2010-01-28 Roche Molecular Systems, Inc. Excitation and Imaging Optics for Fluorescence Detection
US20150323462A1 (en) * 2014-05-12 2015-11-12 Cellomics, Inc. Automated imaging of chromophore labeled samples
US20160216503A1 (en) * 2015-01-26 2016-07-28 California Institute Of Technology Array level fourier ptychographic imaging

Non-Patent Citations (3)

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Title
ATA TUNA CIFTLIK 等: "Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs", 《PNAS》, vol. 110, no. 14, pages 5363 - 5368, XP055432225, DOI: 10.1073/pnas.1211273110 *
JOHN H WITTIG JR 等: "A reusable microfluidic plate with alternate-choice architecture for assessing growth preference in tissue culture", 《JOURNAL OF NEUROSCIENCE METHODS》, vol. 144, no. 1, pages 79 - 89 *
顾月清 等: "《全国普通高等院校生物医学工程规划教材 生物医学工程技术》", 31 July 2017, 北京：中国医药科技出版社, pages: 212 - 216 *

Also Published As

Publication number Publication date
JP2024023740A (ja) 2024-02-21
US20250164475A1 (en) 2025-05-22
US12235264B2 (en) 2025-02-25
WO2019226897A3 (en) 2019-12-26
JP7489326B2 (ja) 2024-05-23
EP3803397A2 (en) 2021-04-14
US20210215687A1 (en) 2021-07-15
JP7827683B2 (ja) 2026-03-10
JP2021524911A (ja) 2021-09-16
WO2019226897A2 (en) 2019-11-28

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