CN112433050A - 一种肺炎衣原体IgM抗体检测试剂盒及其检测方法 - Google Patents
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Abstract
本发明公开了一种肺炎衣原体IgM抗体检测试剂盒及其检测方法,包括磁微粒混悬浮液,酶标工作液、样品稀释液和校准液;所述的磁微粒混悬浮液中含有鼠抗人IgM(μ)链单克隆抗体;所述酶标工作液中含有HRP标记的肺炎衣原体基因工程抗原;所述校准液采用肺炎衣原体IgM高效价抗体阳性血清稀释制成;所述校准液含有中含有CP‑IgM抗体。本发明能够对人体内的肺炎衣原体IgM抗体进行快速检测并具有检测效果好检测准确度高的特点。
Description
技术领域
本发明涉及医疗检测技术领域,具体为一种肺炎衣原体IgM抗体检测试剂盒及其检测方法。
背景技术
肺炎衣原体(Chlamydia pneumoniae,Cpn)作为衣原体属的一种重要的呼吸道病原微生物,自从上个世纪80年代中期由Grayston 等人正式命名,在随后的时间里被研究者们广泛研究。现仅知人是该病原体的宿主,感染方式可能为人与人之间通过呼吸道分泌物传播。5岁以下儿童极少受染,8岁以上儿童及青年易被感染,尤其是人群聚集处,如家庭、学校、兵营中易流行,经血清流行病学调查,证实成人中至少有40%已受到该衣原体感染,大部分为亚临床型。肺炎衣原体是专性细胞内寄生的微生物,寄居于人呼吸道和咽喉等处,在儿童和成人中产生上呼吸道和呼吸道感染,常引起肺炎和支气管炎等呼吸道疾病。目前研究表明,其还与动脉粥样硬化综合症、老年痴呆症、心肌炎、哮喘等疾病有关。临床上由于其与其他呼吸道病原体引起的感染症状类似,因此常难以根据临床表现、x-射线检查等得出结论,确诊往往依赖于实验室诊断。快速有效的诊断方法应该是在疾病的发病初期就可以得到明确的诊断,便于实施针对性的治疗,阻止病情的发展迁延。在人肺炎衣原体感染的实验室诊断上,被检者血清中抗人肺炎衣原体抗体指标则是诊断结果的重要依据之一。因此我们提供一种肺炎衣原体IgM抗体检测试剂盒。
发明内容
为解决现有技术存在的缺陷,本发明提供一种肺炎衣原体IgM抗体检测试剂盒及其检测方法。
为了解决上述技术问题,本发明提供了如下的技术方案:
本发明一种肺炎衣原体IgM抗体检测试剂盒,包括磁微粒混悬浮液,酶标工作液、样品稀释液和校准液;
所述的磁微粒混悬浮液中含有鼠抗人IgM(μ)链单克隆抗体;所述酶标工作液中含有HRP标记的肺炎衣原体基因工程抗原;所述校准液采用肺炎衣原体IgM高效价抗体阳性血清稀释制成;所述校准液含有中含有CP-IgM抗体。
一种肺炎衣原体IgM抗体检测试剂盒的检测方法,包括以下几个步骤,
步骤1:向反应容器内的样本孔内加入样品稀释液100μL/孔,每孔分别加入校准品或样本10μL,每孔分别加入磁微粒混悬液20μL;
步骤2:混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤3:加300μL清洗液至检测管中并混匀,并磁分离去上清;
步骤4:重复步骤3三遍,然后在反应容器内样本孔的每孔分别加入酶结合物100μL,混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤5:重复步骤4三遍,每孔加入发光底物A液和发光底物B 液各50μL,混匀后1~5分钟检测发光强度。
步骤6:利用分析仪将根据检测信号和标准曲线自动计算出各样本中被测物浓度,根据测物浓度判断感染状况。
作为本发明的一种优选技术方案,所述计算出各样本中被测物浓度的方法是,以校准品发光强度RLU值的log值为y轴建立定标曲线,得到曲线方程Y=m(X)+c及其线性相关系数R,将所读样品 RLU值的对数(Y)代入上述曲线方程,求得对应的X值,再求反对数后,即为所测样品中CP-IgM抗体的浓度。
作为本发明的一种优选技术方案,在样本检测前通过校准品对标准曲线进行线性拟合,当标准曲线的线性相关系数不小于0.990时,同时使用两个分析物水平的质控品(CP-IgM2和CP-IgM5)进行质量控制,测试结果在质控范围即±15%范围内,方可进行样本的检测。
作为本发明的一种优选技术方案,当分析仪将根据检测信号和标准曲线自动计算出样本浓度值<20AU/mL判为阴性;浓度值≥25AU/mL 判为阳性;20AU/mL≤浓度值<25AU/mL判为可疑。
本发明的有益效果是:该种肺炎衣原体IgM抗体检测试剂盒,本发明能够对人体内的肺炎衣原体IgM抗体进行快速检测并具有检测效果好检测准确度高的特点。能快速的检测准确度稿的特点,其中当分析仪将根据检测信号和标准曲线自动计算出样本浓度值<20AU/mL 判为阴性;浓度值≥25AU/mL判为阳性;20AU/mL≤浓度值<25AU/mL 判为可疑。
具体实施方式
以下结合本发明的优选实施例进行说明,应当理解,此处所描述的优选实施例仅用于说明和解释本发明,并不用于限定本发明。
实施例:本发明一种肺炎衣原体IgM抗体检测试剂盒,包括磁微粒混悬浮液,酶标工作液、样品稀释液和校准液;
所述的磁微粒混悬浮液中含有鼠抗人IgM(μ)链单克隆抗体;所述酶标工作液中含有HRP标记的肺炎衣原体基因工程抗原;所述校准液采用肺炎衣原体IgM高效价抗体阳性血清稀释制成;所述校准液含有中含有CP-IgM抗体。
一种肺炎衣原体IgM抗体检测试剂盒的检测方法,包括以下几个步骤,
步骤1:向反应容器内的样本孔内加入样品稀释液100μL/孔,每孔分别加入校准品或样本10μL,每孔分别加入磁微粒混悬液20μL;
步骤2:混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤3:加300μL清洗液至检测管中并混匀,并磁分离去上清;
步骤4:重复步骤3三遍,然后在反应容器内样本孔的每孔分别加入酶结合物100μL,混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤5:重复步骤4三遍,每孔加入发光底物A液和发光底物B 液各50μL,混匀后1~5分钟检测发光强度。
步骤6:利用分析仪将根据检测信号和标准曲线自动计算出各样本中被测物浓度,根据测物浓度判断感染状况。
所述计算出各样本中被测物浓度的方法是,以校准品发光强度 RLU值的log值为y轴建立定标曲线,得到曲线方程Y=m(X)+c 及其线性相关系数R,将所读样品RLU值的对数(Y)代入上述曲线方程,求得对应的X值,再求反对数后,即为所测样品中CP-IgM抗体的浓度。
在样本检测前通过校准品对标准曲线进行线性拟合,当标准曲线的线性相关系数不小于0.990时,同时使用两个分析物水平的质控品 (CP-IgM2和CP-IgM5)进行质量控制,测试结果在质控范围即±15%范围内,方可进行样本的检测。
当分析仪将根据检测信号和标准曲线自动计算出样本浓度值<20 AU/mL判为阴性;浓度值≥25AU/mL判为阳性;20AU/mL≤浓度值<25 AU/mL判为可疑。
本发明的有益效果是:该种肺炎衣原体IgM抗体检测试剂盒,本发明能够对人体内的肺炎衣原体IgM抗体进行快速检测并具有检测效果好检测准确度高的特点。能快速的检测准确度稿的特点,其中当分析仪将根据检测信号和标准曲线自动计算出样本浓度值<20AU/mL 判为阴性;浓度值≥25AU/mL判为阳性;20AU/mL≤浓度值<25AU/mL 判为可疑。
最后应说明的是:以上所述仅为本发明的优选实施例而已,并不用于限制本发明,尽管参照前述实施例对本发明进行了详细的说明,对于本领域的技术人员来说,其依然可以对前述各实施例所记载的技术方案进行修改,或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
Claims (5)
1.一种肺炎衣原体IgM抗体检测试剂盒,其特征在于:包括磁微粒混悬浮液,酶标工作液、样品稀释液和校准液;
所述的磁微粒混悬浮液中含有鼠抗人IgM(μ)链单克隆抗体;所述酶标工作液中含有HRP标记的肺炎衣原体基因工程抗原;所述校准液采用肺炎衣原体IgM高效价抗体阳性血清稀释制成;所述校准液含有中含有CP-IgM抗体。
2.根据权利要求1所述的一种肺炎衣原体IgM抗体检测试剂盒的检测方法,其特征在于,包括以下几个步骤,
步骤1:向反应容器内的样本孔内加入样品稀释液100μL/孔,每孔分别加入校准品或样本10μL,每孔分别加入磁微粒混悬液20μL;
步骤2:混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤3:加300μL清洗液至检测管中并混匀,并磁分离去上清;
步骤4:重复步骤3三遍,然后在反应容器内样本孔的每孔分别加入酶结合物100μL,混匀后37±0.5℃温育15分钟,并磁分离去上清;
步骤5:重复步骤4三遍,每孔加入发光底物A液和发光底物B液各50μL,混匀后1~5分钟检测发光强度。
步骤6:利用分析仪将根据检测信号和标准曲线自动计算出各样本中被测物浓度,根据测物浓度判断感染状况。
3.根据权利要求2所述的一种肺炎衣原体IgM抗体检测试剂盒的检测方法,其特征在于,所述计算出各样本中被测物浓度的方法是,以校准品发光强度RLU值的log值为y轴建立定标曲线,得到曲线方程Y=m(X)+c及其线性相关系数R,将所读样品RLU值的对数(Y)代入上述曲线方程,求得对应的X值,再求反对数后,即为所测样品中CP-IgM抗体的浓度。
4.根据权利要求2所述的一种肺炎衣原体IgM抗体检测试剂盒的检测方法,其特征在于,在样本检测前通过校准品对标准曲线进行线性拟合,当标准曲线的线性相关系数不小于0.990时,同时使用两个分析物水平的质控品(CP-IgM2和CP-IgM5)进行质量控制,测试结果在质控范围即±15%范围内,方可进行样本的检测。
5.根据权利要求2所述的一种肺炎衣原体IgM抗体检测试剂盒的检测方法,其特征在于,当分析仪将根据检测信号和标准曲线自动计算出样本浓度值<20AU/mL判为阴性;浓度值≥25AU/mL判为阳性;20AU/mL≤浓度值<25AU/mL判为可疑。
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08327630A (ja) * | 1995-03-28 | 1996-12-13 | Hitachi Chem Co Ltd | クラミジア・ニューモニエ抗原、その製造法、それを用いた抗クラミジア・ニューモニエ抗体の測定法及び抗クラミジア・ニューモニエ抗体測定用試薬 |
WO2008021862A2 (en) * | 2006-08-08 | 2008-02-21 | Binax, Inc. | Methods and compositions for the detection of mycoplasma pneumoniae |
CN101551395A (zh) * | 2008-04-02 | 2009-10-07 | 北京科美东雅生物技术有限公司 | 戊型肝炎病毒IgM抗体化学发光免疫测定试剂盒及其制备方法 |
WO2013008743A1 (ja) * | 2011-07-11 | 2013-01-17 | 富士レビオ株式会社 | クラミジアニューモニエ抗原及びその利用 |
CN106404755A (zh) * | 2016-06-30 | 2017-02-15 | 深圳市亚辉龙生物科技股份有限公司 | 一种肺炎支原体IgM化学发光免疫检测试剂盒及其制备方法 |
CN109444427A (zh) * | 2018-12-12 | 2019-03-08 | 郑州安图生物工程股份有限公司 | 一种检测人细小病毒IgM抗体的试剂盒 |
CN111337682A (zh) * | 2020-05-18 | 2020-06-26 | 博奥赛斯(天津)生物科技有限公司 | 一种新型冠状病毒IgM/IgG磁微粒化学发光免疫检测试剂盒 |
-
2020
- 2020-11-04 CN CN202011214298.8A patent/CN112433050A/zh active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08327630A (ja) * | 1995-03-28 | 1996-12-13 | Hitachi Chem Co Ltd | クラミジア・ニューモニエ抗原、その製造法、それを用いた抗クラミジア・ニューモニエ抗体の測定法及び抗クラミジア・ニューモニエ抗体測定用試薬 |
WO2008021862A2 (en) * | 2006-08-08 | 2008-02-21 | Binax, Inc. | Methods and compositions for the detection of mycoplasma pneumoniae |
CN101551395A (zh) * | 2008-04-02 | 2009-10-07 | 北京科美东雅生物技术有限公司 | 戊型肝炎病毒IgM抗体化学发光免疫测定试剂盒及其制备方法 |
WO2013008743A1 (ja) * | 2011-07-11 | 2013-01-17 | 富士レビオ株式会社 | クラミジアニューモニエ抗原及びその利用 |
CN106404755A (zh) * | 2016-06-30 | 2017-02-15 | 深圳市亚辉龙生物科技股份有限公司 | 一种肺炎支原体IgM化学发光免疫检测试剂盒及其制备方法 |
CN109444427A (zh) * | 2018-12-12 | 2019-03-08 | 郑州安图生物工程股份有限公司 | 一种检测人细小病毒IgM抗体的试剂盒 |
CN111337682A (zh) * | 2020-05-18 | 2020-06-26 | 博奥赛斯(天津)生物科技有限公司 | 一种新型冠状病毒IgM/IgG磁微粒化学发光免疫检测试剂盒 |
Non-Patent Citations (1)
Title |
---|
杨建丽;苗拥军;吴学炜;鲁清月;陶占领;杨俊梅;刘功成;: "肺炎支原体IgM抗体三种检测方法的临床应用比较", 医药论坛杂志, no. 02, pages 4 - 7 * |
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