CN112410256B - Paenibacillus with yield increasing effect and preparation method and application thereof - Google Patents
Paenibacillus with yield increasing effect and preparation method and application thereof Download PDFInfo
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- CN112410256B CN112410256B CN202011333077.2A CN202011333077A CN112410256B CN 112410256 B CN112410256 B CN 112410256B CN 202011333077 A CN202011333077 A CN 202011333077A CN 112410256 B CN112410256 B CN 112410256B
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- 241000179039 Paenibacillus Species 0.000 title claims abstract description 15
- 230000001965 increasing effect Effects 0.000 title abstract description 28
- 238000002360 preparation method Methods 0.000 title abstract description 12
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241001337872 Paenibacillus chitinolyticus Species 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 239000002689 soil Substances 0.000 abstract description 9
- 239000003337 fertilizer Substances 0.000 abstract description 8
- 238000000855 fermentation Methods 0.000 abstract description 7
- 230000004151 fermentation Effects 0.000 abstract description 7
- 239000003899 bactericide agent Substances 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 3
- 239000013043 chemical agent Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 12
- 230000008569 process Effects 0.000 description 10
- 230000012010 growth Effects 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000234314 Zingiber Species 0.000 description 5
- 235000006886 Zingiber officinale Nutrition 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- 235000008397 ginger Nutrition 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 244000061458 Solanum melongena Species 0.000 description 3
- 235000002597 Solanum melongena Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Ecology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention relates to the technical field of paenibacillus and application thereof, and provides paenibacillus with yield increasing effect, a preparation method and application thereof. The invention overcomes the defects of the prior art, solves the problems that the existing fertilizer application and the use of pesticides and bactericides lead to yield increase of crops, soil is easy to damage, chemical agents can remain on crops and the yield increase effect is affected, and the invention can improve the yield increase effect on various crops by adopting a bacillus-like bacterium and preparing the bacillus-like bacterium into the microbial inoculum, has the advantages of simple preparation, short fermentation time, convenient application, high yield increase efficiency, and the like, and simultaneously has the advantages of increasing the yield of crops, improving the quality of crops, improving the soil, protecting the ecological environment, reducing pollution and the like.
Description
Technical Field
The invention relates to the technical field of paenibacillus and application thereof, in particular to paenibacillus with yield increasing effect, and a preparation method and application thereof.
Background
Crop yields, including crop yields, have been a worldwide problem. At present, fertilization and use of pesticides and bactericides are generally adopted in the agricultural production process to increase the yield of crops.
The long-term use of chemical fertilizer can destroy the aggregate structure of soil due to the lack of organic matters and humus in soil, so that the soil is hardened and the yield of agricultural plants is reduced. In addition, the quality of vegetables, melons and fruits can be greatly reduced by using a large amount of chemical fertilizers. Because crops not only need nitrogen, phosphorus and potassium, but also need a plurality of microelements such as calcium, iron, zinc, selenium and the like, and the general components of the fertilizer are relatively single, long-term use of the fertilizer necessarily leads to the fact that the nutrients contained in soil tend to be single, and the crops are easy to be maladjusted, so that the internal transformation and synthesis of the crops are blocked, the quality of the crops is necessarily reduced, and the crops are not sweet and are not fragrant.
The pesticide and bactericide are used for helping the sterilization and disinsection of the leaf surfaces of crops to protect the crops, but some toxic and harmful substances are absorbed into the crops along with the roots, stems and leaves of the crops, and some harmful substances remain on the agricultural products and harm the health of people. In addition, the process of using the insecticidal and bactericidal agent can also pollute the environment.
Safe, green, pollution-free crops are a worldwide problem sought to be solved.
The microbial fertilizer has the advantages of regulating plant growth, increasing crop yield, improving crop quality, improving soil, protecting ecological environment, reducing pollution and the like.
Aiming at the growth mode of crops, a strain capable of promoting the yield increase of the crops is screened and found, so that the damage caused by using a large amount of chemical fertilizers and using pesticides at multiple frequencies can be solved in the yield increase process of the crops, and the green and sustainable agricultural targets are realized.
Therefore, we propose a paenibacillus with yield increasing effect, and a preparation method and application thereof.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides the paenibacillus with the yield increasing effect, the preparation method and the application thereof, overcomes the defects of the prior art, has reasonable design and compact structure, solves the problems that the existing fertilizer application and the use of pesticides and bactericides lead to the yield increase of crops, the soil is easy to be damaged, and the chemical agents can remain on the crops to influence the yield increasing effect.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme:
a bacillus species, which strain has been deposited in a deposit unit designated by the national intellectual property agency, on the date of deposit: 2019, 7 and 3, deposit unit name: the Guangdong province microorganism strain collection center, collection unit address: first, the middle road 100 # laboratory building 5 in the Vietnam city, guangzhou, guangdong province, with the preservation number: GDMCC NO 60710, classified as Paenibacillus chiti nolyticus UMBR 0002.
Further, the time for the paenibacillus to enter the logarithmic phase is 4 hours, the proper growth temperature is 20-37 ℃, and the proper growth pH is as follows: 6.0-8.0.
Furthermore, the bacillus-like bacteria is used for increasing the yield of crops, and is used for preparing a microbial inoculum which can effectively promote the yield increase of plants.
Further, the preparation method of the crop yield increasing microbial agent comprises the following steps:
step one: the strain is streaked on LB solid medium for activation culture at 20-37 ℃.
Step two: single colony is selected and inoculated into LB liquid medium, and cultured for 1-3d at the temperature of 20-37 ℃ and the speed of 100-300r/min by a shaking table.
Step three: the strain liquid fermentation broth after fermentation can be directly diluted for use.
(III) beneficial effects
The embodiment of the invention provides paenibacillus with yield increasing effect, and a preparation method and application thereof. The beneficial effects are as follows:
1. the strain has obvious yield increasing effect on crops, simple preparation of the microbial inoculum, short fermentation time and convenient application.
2. The strain replaces the traditional chemical raw materials, and the yield increasing process is safe, green and pollution-free.
3. Increasing crop yield, improving crop quality, improving soil, protecting ecological environment, reducing pollution, etc.
Drawings
FIG. 1 is a bar graph of the yield increasing effect of the above strains on ginger.
FIG. 2 is a bar graph of the yield increasing effect of the above strain on eggplants.
FIG. 3 is a bar graph showing the yield increasing effect of the strain on capsicum.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to FIGS. 1 to 3, a Bacillus species which has been deposited at a deposit unit designated by the national intellectual property agency, date of deposit: 2019, 7 and 3, deposit unit name: the collection of microorganism strains in Guangdong province, collection number: GDMCC NO 60710, classified as Paenibacillus chiti nolyticus UMBR 0002.
The paenibacillus is cultivated into the logarithmic phase for 4 hours, the proper growth temperature is 20-37 ℃, and the proper growth pH is as follows: 6.0-8.0.
The application of the paenibacillus to crop yield increase is characterized in that the paenibacillus is utilized to prepare a microbial inoculum which can effectively promote the yield increase of plants.
A preparation method of a crop yield-increasing microbial agent comprises the following steps:
step one: the strain is streaked on LB solid medium for activation culture at 20-37 ℃.
Step two: single colony is selected and inoculated into LB liquid medium, and cultured for 1-3d at the temperature of 20-37 ℃ and the speed of 100-300r/min by a shaking table.
Step three: the strain liquid fermentation broth after fermentation can be directly diluted for use.
Embodiment one:
experimental materials: ginger, bacterial strain inoculant and LB culture medium
Experiment setting: water control, 1ml LB blank, and 1ml of the above microbial inoculum treatment. Each group was repeated 5 times, with the inoculum being irrigated every 2 weeks.
The experimental process comprises the following steps: in the experimental case, after 10 times of fungus pouring, the ginger is ripe, the ginger is pulled out and cleaned for 4 months, the yield of each plant is measured, and the average plant yield of each group is calculated.
In the case, the growth and development conditions of the three groups are not different, but when the average yield of each plant is measured finally, 1ml of the microbial inoculum is applied, and compared with a 1ml LB blank control group, the yield is increased by 45.29%; the yield is increased by 37.97% compared with the water control group.
Embodiment two:
experimental materials: eggplant, bacterial agent of the bacterial strain and LB culture medium
Experiment setting: as a water control group, a 10ml LB blank group and 10ml of the above microbial inoculum treatment group. Each group was repeated 5 times, with the inoculum being irrigated every 2 weeks.
The experimental process comprises the following steps: in this experimental case, the fungus was co-watered 10 times for 3 months, the fruits were co-picked 4 times, all the fruits were picked up each time, the yield of each plant was measured, and the average plant yield of each group was calculated.
In the case, the growth and development conditions of the three groups are not different, but when the average yield of each plant is measured finally, 1ml of the microbial inoculum is applied, and compared with a 1ml LB blank control group, the yield is increased by 45.29%; the yield is increased by 37.97% compared with the water control group.
Embodiment III:
experimental materials: chili, bacterial agent of the bacterial strain and LB culture medium
Experiment setting: 1ml of LB blank, 1ml of the above-mentioned microbial inoculum treatment group, 10ml of LB blank, and 10ml of the above-mentioned microbial inoculum treatment group were used. Each group was repeated 5 times, with the inoculum being irrigated every 2 weeks.
The experimental process comprises the following steps: in this experimental case, the bacteria were co-watered 10 times for 4 months, the fruits were co-picked 4 times, all the fruits were picked up each time, the yield of each plant was measured, and the average plant yield of each group was calculated.
In the case, the growth and development conditions of the 4 groups are not different, but when the average yield of each plant is measured finally, 1ml of the microbial inoculum is applied, and compared with a 1ml LB blank control group, the yield is increased by 40.19%; the yield of the group with 10ml of the microbial inoculum is increased by 39.04% compared with that of the blank group with 10ml of LB.
Summary of data:
the yield increase effect of the strain on ginger is up to 45.29% compared with that of a blank culture medium control group; the yield is increased by 37.97% compared with the water control group.
The yield increase effect of the strain on the eggplant is up to 31.70% compared with that of a blank culture medium control group; the yield is increased by 56.12% compared with the water control group.
The yield increasing effect of the strain on green pepper is up to 40.19% compared with that of a blank culture medium control group.
The strain has obvious yield increasing effect on crops, simple preparation of the microbial inoculum, short fermentation time and convenient application.
It should be noted that the optimal microbial inoculum usage amount of each crop is different, and the higher the microbial inoculum addition usage amount is, the larger the crop yield is. The optimal usage amount of different crops can be explored according to the habits of the different crops.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (1)
1. The application of the paenibacillus to crop yield increase is characterized in that the paenibacillus is utilized to prepare a microbial inoculum which can effectively promote the yield increase of plants;
the bacillus species has been deposited in a deposit unit designated by the national intellectual property agency, date of deposit: 2019, 7 and 3, deposit unit name: the collection of microorganism strains in Guangdong province, collection number: GDMCC NO 60710, classified as Paenibacillus chiti nolyticus UMBR 0002.
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| CN111235133B (en) * | 2019-09-30 | 2023-09-22 | 广西民族大学 | Bacillus chitin-philic chitinase gene and clone expression and application thereof |
| CN110699276A (en) * | 2019-09-30 | 2020-01-17 | 广西民族大学 | Strain of chitin-like paenibacillus and application thereof |
Citations (4)
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| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
| CN110699276A (en) * | 2019-09-30 | 2020-01-17 | 广西民族大学 | Strain of chitin-like paenibacillus and application thereof |
| CN111235133A (en) * | 2019-09-30 | 2020-06-05 | 广西民族大学 | Chitinase gene of chitin-like paenibacillus and cloning expression and application thereof |
| CN116348589A (en) * | 2020-08-03 | 2023-06-27 | 巴斯夫欧洲公司 | Materials and methods for improving plant health |
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| CA2920729A1 (en) * | 2013-08-12 | 2015-02-19 | Bio-Cat Microbials Llc | Compositions comprising bacillus strains and methods of use to suppress the activities and growth of fungal plant pathogens |
| US20200131096A1 (en) * | 2018-10-29 | 2020-04-30 | Sustainable Community Development, Llc | Biofertilizer Composition and Method of Manufacture |
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| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
| CN110699276A (en) * | 2019-09-30 | 2020-01-17 | 广西民族大学 | Strain of chitin-like paenibacillus and application thereof |
| CN111235133A (en) * | 2019-09-30 | 2020-06-05 | 广西民族大学 | Chitinase gene of chitin-like paenibacillus and cloning expression and application thereof |
| CN116348589A (en) * | 2020-08-03 | 2023-06-27 | 巴斯夫欧洲公司 | Materials and methods for improving plant health |
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| Title |
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| Cong Liu等.Cloning,expression and characterization of a chitinase from Paenibacillus chitinolyticus strain UMBR ooo2.Biochemistry,Biophysics and Molecular Biology.2020,第1-23页. * |
| Production of Enzymes by Paenibacillus chitinolyticus and Paenibacillus echimensis to Obtain Chitooligosaccharides;Nathalia Kelly de Araujo等;Applied Biochemistry and Biotechnology;第292-300页 * |
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