CN112410256A - Paenibacillus with yield increasing effect and preparation method and application thereof - Google Patents
Paenibacillus with yield increasing effect and preparation method and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to the technical field of paenibacillus and application thereof, and provides paenibacillus with a yield increasing effect, a preparation method and application thereof. The invention overcomes the defects of the prior art, solves the problems that the prior fertilizer application and the use of the insecticide and the bactericide lead the crop to increase the yield, easily damages soil, and simultaneously, chemical agents also remain on the crops to influence the yield increasing effect.
Description
Technical Field
The invention relates to the technical field of paenibacillus and application thereof, in particular to paenibacillus with a yield increasing effect and a preparation method and application thereof.
Background
The production of crops, including agricultural crops, has long been a worldwide problem. At present, in the agricultural production process, the crop yield is increased by applying fertilizer and using insecticide and bactericide.
After the chemical fertilizer is used for a long time, the soil is lack of organic matters and humus, so that the granular structure of the soil is damaged, the soil is hardened, and the yield of agricultural plants is reduced. In addition, the quality of vegetables, melons and fruits is greatly reduced by using a large amount of fertilizer. Because crops not only need nitrogen, phosphorus and potassium, but also need a plurality of microelements such as calcium, iron, zinc, selenium and the like, and the general components of the chemical fertilizer are single, long-term use of the chemical fertilizer inevitably leads to single nutrient in soil, and the crop is easy to be out of nutrition, thereby leading to the internal transformation and synthesis of the crops to be blocked, inevitably leading to the quality reduction of the crops, the sweet melon and the fragrant vegetable.
The action mechanism of the pesticide and the bactericide is that the bactericide and the bactericide are used for helping the foliage of crops to sterilize and kill the pests so as to protect the crops, but some toxic and harmful substances are absorbed into the bodies of the crops along with roots, stems and leaves of the crops, and some toxic and harmful substances remain on agricultural products and harm the health of people. In addition, the process of using the pesticide and the bactericide can cause environmental pollution.
Safe, green, pollution-free crops are a problem addressed worldwide.
The microbial fertilizer has the advantages of regulating plant growth, increasing crop yield, improving crop quality, improving soil, protecting ecological environment, reducing pollution and the like.
Aiming at the growth mode of crops, a strain capable of promoting the yield increase of the crops is screened and searched, so that the harm caused by using a large amount of chemical fertilizer and using pesticides at multiple frequencies can be solved in the yield increase process of the crops, and the sustainable green and friendly goal of agriculture is realized.
Therefore, the Paenibacillus with the yield increasing effect and the preparation method and the application thereof are provided.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the paenibacillus with the effect of increasing the yield, the preparation method and the application thereof, overcomes the defects of the prior art, has reasonable design and compact structure, solves the problems that the yield of crops is increased by using the prior fertilizer application and the prior insecticide and bactericide, soil is easy to damage, and chemical agents also remain on the crops to influence the yield increasing effect.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
a bacillus specie which has been deposited in a depository designated by the national intellectual property office on a date of deposit: 7/3/2019, depository name: guangdong province microbial strain preservation center, preservation unit address: experimental building 5, storage number: GDMCC NO:60710, classified and named as Paenibacillus chili nomycotics UMBR 0002.
Further, the time of the Paenibacillus culture entering logarithmic growth phase is 4h, the suitable growth temperature is 20-37 ℃, and the suitable growth pH is as follows: 6.0-8.0.
Further, the paenibacillus is used for increasing the yield of crops, and the paenibacillus is prepared into a microbial inoculum which can effectively promote the yield of the plants.
Further, the preparation method of the crop yield increasing microbial inoculum comprises the following steps:
the method comprises the following steps: the strain is streaked on an LB solid culture medium and activated and cultured at the temperature of 20-37 ℃.
Step two: picking single colony to inoculate into LB liquid culture medium, shaking table 20-37 deg.C, 100-300r/min for 1-3 d.
Step three: the fermented liquid of the strain can be directly diluted for use.
(III) advantageous effects
The embodiment of the invention provides paenibacillus with a yield increasing effect and a preparation method and application thereof. The method has the following beneficial effects:
1. the strain has obvious yield increasing effect on crops, simple preparation of the microbial inoculum, short fermentation time and convenient application.
2. The strain replaces the traditional chemical raw materials, and the yield increasing process is safe, green and pollution-free.
3. Increasing crop yield and improving crop quality, improving soil, protecting ecological environment, reducing pollution, etc.
Drawings
FIG. 1 is a bar graph showing the effect of the above strains on ginger production increase.
FIG. 2 is a bar graph of the yield increasing effect of the strain on eggplant.
FIG. 3 is a bar graph showing the effect of the above strains on the increase in yield of pepper.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to FIGS. 1-3, a Bacillus species deposited in a depositary unit designated by the national intellectual Property office on a date of deposit: 7/3/2019, depository name: guangdong province microorganism strain preservation center, preservation number: GDMCC NO:60710, classified and named as Paenibacillus chili nomycotics UMBR 0002.
The time of the paenibacillus culture entering logarithmic growth phase is 4h, the suitable growth temperature is 20-37 ℃, and the suitable growth pH is as follows: 6.0-8.0.
The application of the paenibacillus in crop yield increase is characterized in that the paenibacillus is used for preparing a microbial inoculum, and the microbial inoculum can effectively promote the yield increase of plants.
A preparation method of a crop yield increasing microbial inoculum comprises the following steps:
the method comprises the following steps: the strain is streaked on an LB solid culture medium and activated and cultured at the temperature of 20-37 ℃.
Step two: picking single colony to inoculate into LB liquid culture medium, shaking table 20-37 deg.C, 100-300r/min for 1-3 d.
Step three: the fermented liquid of the strain can be directly diluted for use.
The first embodiment is as follows:
experimental materials: ginger, bacterial agent of the strain and LB culture medium
Experimental setup: used as water control group, 1ml LB blank control group, 1ml of the above-mentioned bacteria agent treatment group. Each group was repeated 5 times, irrigating the inoculum every 2 weeks.
The experimental process comprises the following steps: in the experimental case, after 10 times of fungus pouring, the ginger is ripe for 4 months, pulled out and cleaned, the yield of each plant is measured, and the average plant yield of each group is calculated.
In the case, the growth and development conditions of the three groups do not show difference, but when the average yield of each plant is measured finally, the yield of the group applied with 1ml of the microbial inoculum is increased by 45.29 percent compared with that of a 1ml LB blank control group; compared with a water control group, the yield is increased by 37.97 percent.
Example two:
experimental materials: eggplant, bacterial agent of the strain and LB culture medium
Experimental setup: the test samples were used as a water control, a 10ml LB blank control and a 10ml microbial inoculum treatment. Each group was repeated 5 times, irrigating the inoculum every 2 weeks.
The experimental process comprises the following steps: in the experimental case, the bacteria are watered for 10 times for 3 months, the fruits are picked for 4 times, all the fruits are picked up each time, the yield of each plant is measured, and the average plant yield of each group is calculated.
In the case, the growth and development conditions of the three groups do not show difference, but when the average yield of each plant is measured finally, the yield of the group applied with 1ml of the microbial inoculum is increased by 45.29 percent compared with that of a 1ml LB blank control group; compared with a water control group, the yield is increased by 37.97 percent.
Example three:
experimental materials: capsicum, bacterial agent of the strain and LB culture medium
Experimental setup: a1 ml LB blank group, a 1ml microbial inoculum treated group, a 10ml LB blank group, and a 10ml microbial inoculum treated group were prepared. Each group was repeated 5 times, irrigating the inoculum every 2 weeks.
The experimental process comprises the following steps: in the experimental case, the bacteria are watered for 10 times for 4 months, the fruits are picked for 4 times, all the fruits are picked up each time, the yield of each plant is measured, and the average plant yield of each group is calculated.
In the case, the growth and development conditions of 4 groups do not show difference, but when the average yield of each plant is measured finally, the yield of the group applied with 1ml of the microbial inoculum is increased by 40.19% compared with that of a 1ml LB blank control group; compared with a 10ml LB blank control group, the group applied with 10ml of the microbial inoculum increases the yield by 39.04%.
Data summarization:
compared with a blank culture medium control group, the yield of the ginger is increased by 45.29%; compared with a water control group, the yield is increased by 37.97 percent.
Compared with a blank culture medium control group, the yield of the strain on the eggplant is increased by 31.70%; the yield was increased 56.12% compared to the water control.
Compared with a blank culture medium control group, the yield of the strain is increased by 40.19%.
The strain has obvious yield increasing effect on crops, simple preparation of the microbial inoculum, short fermentation time and convenient application.
It should be noted that the optimal microbial inoculum usage amount of each crop is different, and not the higher the microbial inoculum addition usage amount is, the larger the crop yield increase is. The optimal use amount of different crops can be researched according to the habits of different crops.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A bacillus specie which has been deposited in a depository designated by the national intellectual property office on a date of deposit: 7/3/2019, depository name: guangdong province microorganism strain preservation center, preservation number: GDMCC NO:60710, classified and named as Paenibacillus chili nomycotics UMBR 0002.
2. A paenibacillus bacterium according to claim 1 wherein: the time of the paenibacillus culture entering logarithmic growth phase is 4h, the suitable growth temperature is 20-37 ℃, and the suitable growth pH is as follows: 6.0-8.0.
3. The application of the paenibacillus in increasing the yield of crops as claimed in claim 1, wherein the paenibacillus is used for preparing a microbial inoculum which can effectively promote the yield increase of plants.
4. A preparation method of a crop yield increasing microbial inoculum comprises the following steps:
the method comprises the following steps: streaking the strain on an LB solid culture medium, and performing activated culture at the temperature of 20-37 ℃;
step two: selecting a single colony to be inoculated into an LB liquid culture medium, and culturing for 1-3d at the temperature of 20-37 ℃ and 300r/min by a shaking table;
step three: the fermented liquid of the strain can be directly diluted for use.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110699276A (en) * | 2019-09-30 | 2020-01-17 | 广西民族大学 | Strain of chitin-like paenibacillus and application thereof |
| CN111235133A (en) * | 2019-09-30 | 2020-06-05 | 广西民族大学 | Chitinase gene of chitin-like paenibacillus and cloning expression and application thereof |
| CN111235133B (en) * | 2019-09-30 | 2023-09-22 | 广西民族大学 | Bacillus chitin-philic chitinase gene and clone expression and application thereof |
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