CN112375670A - Household convenient liquid microbial component detection device and detection method - Google Patents
Household convenient liquid microbial component detection device and detection method Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 87
- 239000007788 liquid Substances 0.000 title claims abstract description 44
- 230000000813 microbial effect Effects 0.000 title claims abstract description 10
- 229920000742 Cotton Polymers 0.000 claims abstract description 106
- 239000003814 drug Substances 0.000 claims abstract description 33
- 244000005700 microbiome Species 0.000 claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 22
- 230000008569 process Effects 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000005192 partition Methods 0.000 claims description 8
- 230000007246 mechanism Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 239000012085 test solution Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 229940079593 drug Drugs 0.000 abstract description 4
- 230000008602 contraction Effects 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 9
- 210000003296 saliva Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000711573 Coronaviridae Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 2
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- 239000002184 metal Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000025157 Oral disease Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
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- 201000011529 cardiovascular cancer Diseases 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract
The invention discloses a portable household device for detecting components and contents of liquid microorganisms, which is used for detecting different types of diseases and indexes. The device comprises a medicine table, a sliding table, a low-speed motor, an object stage, a contraction frame and a control chip; the medicine platform is internally composed of five hollow cylindrical main bodies, and is respectively filled with 5 different detection liquid medicines for detecting five different components in a liquid sample; five centrifuge tubes are fixed in the objective table, and the lower part of the centrifuge tubes is connected with a low-speed motor through a rotating head; the telescopic frame is provided with five clamping grooves with the same size for controlling the cotton swab, and a spring and an electromagnet on the telescopic frame are jointly responsible for fixing or releasing the cotton swab; the control chip can release the medicine dosage in the medicine table in a quantitative mode. The invention carries out efficient and simple batch experiments by sampling and collecting the liquid to be detected by the cotton swab, reacting with the medicine and then comparing with the colorimetric card, measures the microbial components and content of the liquid, improves the detection efficiency, and has wide application and low cost.
Description
Technical Field
The embodiment of the invention provides a microbial detection device, and particularly relates to a household semi-automatic microbial detection device.
Background
Saliva is an important substance for reflecting diseases, and saliva detection has the advantages of being noninvasive, convenient, rapid, easy to be accepted by patients and the like, so that more and more attention is paid to the saliva detection. At present, the relationship between various diseases such as oral diseases, cardiovascular diseases, cancers and the like and corresponding pathogenic bacteria and secretions thereof is clearly researched, and the research proves that the species and proportion of the flora have good correlation with the diseases. Therefore, the sensitivity and the accuracy of disease detection can be greatly improved by combining the specific microorganisms with certain clinical indexes, and the detection of the abundance or the proportion change can also be used for judging the risk of diseases, helping patients to take treatment measures as soon as possible and avoiding unnecessary pain and burden caused by delay. Meanwhile, the microbial content of other liquid samples such as drinking water can be used as an index for environmental or health monitoring, and basic reference can be made for further component analysis, while the microbial detection of the liquid samples at present needs to be sent to a specific detection department, the flow is complex, the time consumption is relatively serious, and unnecessary cost is increased. The prior art is in order to design a family and adorn convenient liquid microorganism composition detection device, through adding examination testing agent and liquid sample, automatic start detection procedure. The principle that the reagent shows different color changes by utilizing the reaction of microorganism specific protein and corresponding protease solution is utilized, and the existence and the content of the microorganism are judged by the color comparison of a special color comparison card, so that the disease process is indicated or the self and the environment are simply and self-evaluated.
Disclosure of Invention
In view of the above analysis, the present invention aims to provide a semi-automatic household liquid microorganism detection device, which has a wide application range and can be applied to various detection fields such as salivary microorganisms, new coronavirus, escherichia coli in a water sample, and the like.
The purpose of the invention is mainly realized by the following technical scheme:
in a technical aspect of the present invention, a household type microorganism detection apparatus includes: housing, assembly 1, assembly 2, assembly 3, assembly 4, assembly 5.
In the technical scheme of the invention, the shell comprises: frame, slip table, medicine platform, reagent bottle and chip. The device shell is a cuboid, the length is 230mm, the width is 140mm, the height is 200mm, a chip platform is arranged on the left upper side of the outer frame, and the chip platform can be used for the movement of the automatic control device of the instrument and the body 5; the medicine platform is placed to the frame upper right side for input and output detection liquid, detect liquid and deposit in the reagent bottle. Frame downside is the metal track for assembly body 2's removal sets up the buckle respectively in both sides, and the accuracy of being convenient for is controlled assembly body shift position and can be unanimous with top medicine platform or assembly body 5 level.
In the technical solution of the present invention, an assembly body 1 includes: centrifuge tubes and centrifuge tube caps. The centrifuge tube is mainly used for storing samples to be detected and is used as a main container for detection reaction.
In the technical solution of the present invention, the assembly body 2 includes: detection dish, centrifuging tube lid, objective table, revolving stage, shell, baffle, low-speed motor, base and battery constitute. The sliding table is placed on the guide rail, and the buckle at the bottom of the sliding table is matched with the groove of the guide rail, so that the sliding table and the guide rail can slide relatively. The base and the battery are placed on the sliding table and are composed of a cylinder and a base. The cylinder is that the customization battery is used for supplying power, places the chip control whole device in the middle of the cylinder, and there are six bulges on the base cylinder periphery for the fixed shell, as shown in the figure. The shell is a hollow cylinder, a big hole is arranged in the middle of the bottom of the shell and used for inserting and fixing a cylinder battery, 5 slots are arranged around the big hole and used for placing 5 centrifuge tubes, and 6 holes are formed in the bottom of the shell and matched with the base. The position of 5 centrifuging tubes matches with 5 hole sites of baffle, makes the baffle can descend to suitable position along the centrifuging tube. The groove on the clapboard is used for placing a low-speed motor, and the power supply of the motor comes from the base and the battery below. The turntable is placed on the low-speed motor, so that the low-speed motor is ensured to drive the turntable to rotate at a stable speed. The detection dish is placed above the rotary table, and the cross-shaped protrusion on the upper portion of the rotary table is matched with the cross-shaped groove in the bottom of the detection dish, so that the detection dish can rotate along with the rotary table. This is equivalent to the short swab heads rotating in the test dish, thus ensuring that all the short swab heads can be stained with saliva uniformly. The object stage is placed on the upper part of the clapboard and is a cylinder with 6 holes. The middle 1 big hole is used for the detection dish to pass through, and the peripheral 5 small holes are used for 5 centrifuge tubes to pass through. The objective table can be provided with attached 6-hole filter paper, so that saliva is prevented from dripping to pollute the instrument in the process of moving in front, and the objective table is convenient to clean.
In the technical solution of the present invention, the assembly body 3 includes: a telescoping carriage and a cam member. The telescopic frame and the upper cover are fixed by corresponding clamping grooves, the telescopic frame is provided with five clamping grooves with the same size, and the cotton rod clamp is fixed in the corresponding clamping groove of the telescopic frame and is connected with the center by a spring. After the cotton swab clamp is installed, the cam mechanism drives the cotton swab clamp to move through rotation, and therefore the cotton swab stained with liquid to be detected can move.
In the technical solution of the present invention, the assembly body 4 includes: a cotton rod clamp (1) and a cotton rod clamp (2). The cotton rod clamp (1) is connected with the cotton rod clamp (2) through a spring; the cotton swab clamp is opened first, and the cotton swab clamp is closed after the short cotton swab is inserted into the fixed position.
In the technical solution of the present invention, the assembly body 5 includes: the upper cover, steering wheel, cam element, expansion bracket and cotton swab press from both sides. In the assembling process, the upper cover, the steering engine, the cam element, the telescopic frame and the cotton rod clamp are arranged from top to bottom. The telescopic frame and the upper cover are fixed by corresponding clamping grooves, the telescopic frame is provided with five clamping grooves with the same size, and the cotton rod clamp is fixed in the corresponding clamping groove of the telescopic frame and is connected with the center by a spring; the steering engine is powered by a battery, and a corresponding circuit board and a chip are arranged in a control box above the steering engine to control the rotation speed, time and angle of the steering engine and control the steering engine through the control box. The upper cover is linked with the shell through an electromagnet, and the switch of the electromagnet is controlled through the control box, so that the lifting of the electromagnet is controlled; and putting down an assembly body III provided with a short cotton stick, starting a low-speed motor in the assembly body II, and enabling the detection disc to rotate at a constant speed at a reasonable rotating speed. After the rotation is fixed for a certain time (the short cotton swab is dipped in the liquid to be detected), the rotation of the detection disc is stopped, the assembly body III is pulled up, and the magnetic force of the electromagnet is recovered through the control box. And then, the steering engine is started, the cam element starts to rotate under the driving of the steering engine to form a cam mechanism, and the rotating motion of the cam is changed into the linear intermittent and reciprocating motion of the cotton swab clamp. The cotton rod clamp is driven to move around along the clamping groove of the telescopic frame through rotation of the cam element, and the steering engine automatically stops under the control of the chip after the cotton rod clamp moves to the end point. Open the cotton swab clamp afterwards, the short cotton swab falls into the centrifuging tube of assembly body two (base part) automatically, and is fixed with centrifuge tube lid and centrifuging tube. The medicine table consists of a chassis with 5 circular cavities, a supporting shaft and a rotatable pipe cover. The medicine platform can be opened by rotating the tube cover clockwise, and 5 reagent bottles can be respectively placed in the medicine platform. The reagent bottle contains a label, and quantitative detection liquid can be added according to specific requirements. The reagent bottle contains a pressure device, and can quantitatively inject the test solution with the same volume into the reagent bottle. After the liquid to be detected and the reagent are subjected to sufficient color reaction within a specific set time, the result can be obtained by comparing the color comparison card, and preliminary judgment is made. The detection disc, the centrifugal tube and the short cotton swab are taken out, proper treatment is carried out, the steering engine is started again, the steering engine drives the cam element to rotate again, and the cotton swab clamp gradually returns to the original position under the drawing action of the spring.
The steps of using the device include:
s1, placing the centrifugal tube, and adding the liquid to be detected
S2, adding a proper amount of medicine into the reagent bottle and the centrifuge tube
S3, inserting the short cotton rod into the cotton rod clamp
S5, dipping the short cotton stick in the liquid to be detected
S6, dropping the short cotton rod into a centrifuge tube for reaction
S7, properly disposing the discarded waste, cleaning the instrument and finishing the detection
The technical scheme of the invention can at least realize one of the following effects:
1. the kit has wide application, and can be applied to the fields of multi-aspect detection such as salivary microorganisms, new coronavirus, escherichia coli in a water sample and the like;
2. through semi-automatic program design, a user can more easily carry out a series of processes from collecting a detection sample to colorimetric obtaining a result and even handing over the result to a related mechanism, the program is simplified, and the time is saved;
3. the detection reagent has consistent specification and is sold together with the device, so that the problem of detection errors caused by different dosages is greatly reduced;
4. the low-speed motor and the battery adopted by the invention ensure the good endurance capacity of the device;
5. the assembly body 5 designed by the invention is easy to operate, the unnecessary contact process is greatly reduced, the detection safety is improved, and meanwhile, the pollution of a sample to the device can be prevented by using the matched disposable filter paper;
6. each solution containing container is a disposable container, so that potential infection hazards caused by incomplete cleaning and inaccurate detection conditions of subsequent personnel are avoided;
7. the result color comparison of the invention is easy to carry out, provides a high-efficiency and convenient method for the preliminary detection of the user, and provides convenience for the user and the relative thereof.
In the invention, the technical schemes can be combined with each other to realize more preferable combination schemes. Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
Drawings
The drawings are only for purposes of illustrating particular embodiments and are not to be construed as limiting the invention, wherein like reference numerals are used to designate like parts throughout.
FIG. 1 shows an assembly 1 according to an embodiment of the invention
FIG. 2 shows an assembly 2 according to an embodiment of the invention
FIG. 3 shows an assembly 3 according to an embodiment of the invention
FIG. 4 shows an assembly 4 according to an embodiment of the invention
FIG. 5 shows an assembly 5 according to an embodiment of the invention
FIG. 6 shows an overall structure of an embodiment of the present invention
Reference numerals:
1-short cotton swab, 2-detection dish, 3-centrifuge tube, 4-centrifuge tube cover, 5-objective table, 6-rotary table, 7-shell, 8-base and battery, 9-partition plate, 10-low-speed motor, 11-expansion bracket, 12-cam element, 13-cotton swab clamp (1), 14-cotton swab clamp (2), 15-steering engine, 16-upper cover, 17-outer frame, 18-sliding table, 19-medicine table, 20-reagent bottle and 21-chip.
Detailed Description
The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which form a part hereof, and which together with the embodiments of the invention serve to explain the principles of the invention and not to limit its scope.
In the description of the embodiments of the present invention, it should be noted that the term "connected" is to be understood broadly, and may be, for example, a fixed connection, a detachable connection, or an integrated connection, which may be a mechanical connection, an electrical connection, a direct connection, or an indirect connection via an intermediate medium, unless explicitly stated or limited otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "top," "bottom," "above … …," "below," and "on … …" as used throughout the description are relative positions with respect to components of the device, such as the relative positions of the top and bottom substrates inside the device. It will be appreciated that the devices are multifunctional, regardless of their orientation in space.
As shown in fig. 1 to 6, the embodiment of the present invention provides a novel household-type microorganism detection device, which can easily detect a liquid to be detected at home. The device includes: assembly 1(19), assembly 2 (20), assembly 3(21), assembly 4(22), assembly 5(23) and a housing. The outer frame is a similar cuboid with the length of 230mm, the width of 140mm and the height of 200mm,
during operation, the sliding table (18) is firstly placed on the guide rail, and the buckle at the bottom of the sliding table (18) is matched with the buckle of the guide rail, so that the sliding table (18) and the guide rail can realize relative sliding. The base and the battery (08) are placed on the sliding table and are composed of a cylinder and a base. The cylinder is that the customization battery is used for the power supply, places chip (21) control whole device in the middle of the cylinder, and there are six bulges on the base cylinder periphery for fixed shell (07). The shell (07) is a hollow cylinder, a big hole is arranged in the middle of the bottom of the shell and used for inserting and fixing a cylinder battery, 5 slots are arranged around the big hole and used for placing 5 centrifuge tubes (03), and 6 holes are arranged at the bottom of the shell and matched with the base. The positions of the 5 centrifugal tubes (03) are matched with the 5 holes of the partition plate (09), so that the partition plate (09) can be lowered to a proper position along the centrifugal tubes (03). A low-speed motor (10) is arranged in a groove on the partition plate (09), and the power supply of the motor comes from a base and a battery (08) below. The rotary table (06) is placed on the low-speed motor (10), and the low-speed motor (10) is ensured to drive the rotary table (06) to rotate at a stable speed. The detection disc (02) is placed above the rotary table (06), and the cross-shaped protrusion on the upper portion of the rotary table (06) is matched with the cross-shaped groove in the bottom of the detection disc (02), so that the detection disc (02) can rotate along with the rotary table (06). The object stage (05) is placed on the upper part of the partition plate (09) and is a cylinder with 6 holes. The middle 1 big hole is used for the detection dish (02) to pass through, and the surrounding 5 small holes are used for the 5 centrifugal tubes (03) to pass through. The object stage (05) can be placed with special 6-hole filter paper to prevent the liquid to be detected from dropping to pollute the instrument in the process of moving ahead, and the cleaning is convenient.
Before starting the machine, the installation of a short cotton swab (01) is completed, an assembly body 4 (a cotton swab clamp) for fixing the cotton swab consists of a cotton swab clamp 1(13) and a cotton swab clamp 2(14), and the cotton swab clamp 1(13) and the cotton swab clamp 2(14) are connected through a spring; the cotton swab clamp is opened first, and the short cotton swab (01) is inserted into a fixed position and then closed. After the short cotton swab (01) is fixed, the assembly body II is moved to the corresponding position of the guide rail to be fixed. The fitting body 5 is mainly composed of five parts: the cotton swab comprises a cam element, a cotton swab clamp, a telescopic frame (11), a steering engine (15) and an upper cover (16). In the assembly process, the upper cover (16), the steering engine (15), the cam element (12), the telescopic frame (11) and the cotton swab clamp are arranged from top to bottom. The telescopic frame (11) and the upper cover (16) are fixed by corresponding clamping grooves, the telescopic frame is provided with five clamping grooves with the same size, and the cotton rod clamp is fixed in the corresponding clamping groove of the telescopic frame (11) and is connected with the center by a spring; the steering engine (15) is powered by a battery, and a corresponding circuit board and a chip are arranged in a control box above the steering engine to control the rotation speed, time and angle of the steering engine and control the steering engine through the control box. The upper cover (16) is linked with the shell through an electromagnet, and the switch of the electromagnet is controlled through the control box, so that the lifting of the electromagnet is controlled; and (3) putting down an assembly body III provided with a short cotton stick (01), starting a low-speed motor (10) in the assembly body II, and enabling the detection disc (02) to start to rotate at a reasonable rotating speed at a constant speed. After the test disc (02) is stopped rotating after the test disc rotates for a fixed time (the short cotton swab (01) is dipped in the liquid to be tested), the assembly body III is pulled up, and the magnetic force of the electromagnet is recovered through the control box. And then the steering engine (15) is started, the cam element (12) starts to rotate under the driving of the steering engine (15) to form a cam mechanism, and the rotating motion of the cam is changed into the linear intermittent and reciprocating motion of the cotton swab clamp. The cotton rod clamp is driven to move to the periphery along the clamping groove of the telescopic frame (11) through the rotation of the cam element (12), and the steering engine (15) automatically stops under the control of the chip after the cotton rod clamp moves to the end point. Then the cotton swab clamp is opened, the short cotton swab (01) automatically falls into a centrifuge tube (03) provided with a second base part, and a centrifuge tube cover (04) is fixed with the centrifuge tube (03). The medicine table (16) consists of a chassis containing 5 circular hollow holes, a supporting shaft and a rotatable pipe cover. The medicine table can be opened by rotating the tube cover clockwise, and 5 reagent bottles (17) can be respectively placed in the medicine table. The reagent bottle contains a label, and quantitative detection liquid can be added according to specific requirements. The reagent bottle (17) contains a pressure device, and can quantitatively inject the test solution with the same volume into the reagent bottle (17). After the liquid to be detected and the reagent are subjected to sufficient color reaction within a specific set time, the result can be obtained by comparing the color comparison card, and preliminary judgment is made. The detection disc (02) is taken out, the centrifugal tube and the short cotton swab (01) are properly disposed and discarded, then the steering engine (15) is started again, the cam element (12) is driven by the steering engine (15) to rotate again, and the cotton swab clamp gradually returns to the original position under the drawing action of the spring.
In order to realize semi-automation of the detection process, a chip platform is arranged on the left upper side of the outer frame and can be used for an instrument automation control device to control the movement of the body 5; the medicine platform is arranged on the upper right side of the outer frame and used for inputting and outputting detection liquid. The upper cover (16) is connected with the shell through an electromagnet, and the switch of the electromagnet is controlled through the control box, so that the automatic lifting of the upper cover is controlled; and (3) putting down an assembly body III provided with a short cotton stick (01), starting a low-speed motor (10) in the assembly body II, and enabling the detection disc (02) to start to rotate at a reasonable rotating speed at a constant speed. After the rotation for a fixed time, the rotation of the detection disc (02) is stopped, the assembly body III is pulled up, and the magnetic force of the electromagnet is recovered through the control box. And then the steering engine (15) is started, the cam element (12) starts to rotate under the driving of the steering engine (15) to form a cam mechanism, and the rotating motion of the cam is changed into the linear intermittent reciprocating motion of the cotton swab clamp. The cotton rod clamp is driven to move around along the clamping groove of the telescopic frame (11) through the rotation of the cam element (12), and after the cotton rod clamp moves to the end point, the steering engine (15) automatically stops under the control of the chip. The above processes are controlled by the chip, and then semi-automation of the detection process is realized.
For the convenience that realizes testing process, the frame downside is the metal track for assembly body 2's removal sets up the buckle respectively in both sides, and the assembly body shift position is controlled with top medicine platform (19) or assembly body 5 level unanimity in the accuracy of being convenient for. The telescopic frame (11) and the upper cover (16) are provided with clamping grooves corresponding to the clamping grooves between the telescopic frame (11) and the cotton swab clamp. The cross-shaped protrusion on the upper part of the rotary table (06) is matched with the cross-shaped groove at the bottom of the detection disc (02), so that the detection disc (02) can rotate along with the rotary table (06). Medicine platform (19) and centrifuging tube (03) of below, cotton-tipped rod clamp and centrifuging tube (03) also one-to-one, and direct unclamping spring clamp cotton-tipped rod can fall into corresponding centrifuging tube and begin to detect during the messenger detects. The object stage (05) can be placed with special 6-hole filter paper to prevent the liquid to be detected from dropping to pollute the instrument in the process of moving ahead, and the cleaning is convenient. The reaction obtains the result through simple color comparison, thereby greatly reducing the difficulty of family detection.
The use method of the embodiment of the invention comprises the following steps:
s1, placing a centrifuge tube, adding a solution to be tested:
the assembly body 2 is removed and the centrifuge tube 03 is placed in a hole in the stage 05. And injecting a liquid to be detected into the detection disc 02 and placing the detection disc at a corresponding position of the assembly body 2. The assembly body 2 is placed back on the guide rails and the assembly body 2 is moved until it reaches the snap-fit position below the medicine table.
S2, adding proper medicines into the reagent bottle and the centrifuge tube:
the rotatable cap of the reagent bottle 17 is opened and the required drug is added thereto and then capped. The required amount of the medicine is set, and the control box is started to enable the medicine to flow into the centrifuge tube 03 below.
S3, inserting the short cotton rods into the cotton rod clamps:
the swab clamps 13, 14 are opened and the short swab 01 is inserted into position in both swab clamps. The assembly 2 is moved to the snap-fit position under the assembly 5.
S5, dipping the short cotton stick with the liquid to be detected:
the control box is started, the assembly body 5 descends to a proper position, and then the detection disc 02 starts to rotate to enable the short cotton rod 01 to be stained with liquid to be detected.
S6, dropping the short cotton stick into a centrifuge tube, reacting and judging:
the assembly body 5 automatically ascends, and the short cotton swab 01 moves outwards to the upper part of the centrifuge tube 03. Loosening cotton swab clamps 13 and 14, and allowing the short cotton swab 01 to fall into a centrifuge tube 03 for reaction; the cotton swab grippers 13, 14 are returned to their original positions. The displayed color can be compared with a color comparison card to obtain a preliminary judgment.
S7, properly disposing and discarding waste, cleaning an instrument, and finishing detection:
taking out the detection disc 02, the short cotton rod 01 and the centrifuge tube 03, and properly disposing and discarding; and cleaning the instrument and finishing detection.
In summary, the present invention provides a semi-automatic microorganism detection method. The detection reagent provided by the invention is various and consistent in specification, can be applied to various detection fields such as salivary microorganisms, new coronavirus, escherichia coli in a water sample and the like, and can also reduce the problem of detection errors caused by different specifications; the invention realizes semi-automation through chip control, so that a user can more easily carry out a series of processes from sample collection, sample detection, colorimetric result obtaining and even handover to a relevant mechanism, the program is simplified, and the time is saved; the assembly body 5 designed by the invention is easy to operate, the unnecessary contact process is greatly reduced, the detection safety is improved, and meanwhile, the pollution of a sample to the device can be prevented by using the matched disposable filter paper; each solution container is a disposable container, so that potential infection hazards and subsequent detection errors in the cleaning process are avoided.
Those skilled in the art will appreciate that all or part of the flow of the method implementing the above embodiments may be implemented by a computer program instructing associated hardware, and the program may be stored in a computer readable storage medium. The computer readable storage medium is a magnetic disk, an optical disk, a read-only memory or a random access memory.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (7)
1. The utility model provides a convenient liquid microbiological composition detection device of family dress which characterized in that includes: 1 chip table, 1 assembly 5, 1 assembly 2, 1 outer frame (17), 1 medicine table (19);
the assembly 5 comprises: 1 upper cover (16), 1 steering engine (15), 1 cam element (12), 1 expansion bracket (11) and 5 cotton rod clamps, wherein the cotton rod clamps consist of cotton rod clamps 1(13) and cotton rod clamps 2 (14); the assembly 2 comprises: 5 centrifuging tube (3), 1 objective table (5), 1 detection dish (2), 1 revolving stage (6), 1 shell (7), base and battery (8), 1 slip table (18), 1 chip (21), 1 baffle (9).
2. The convenient liquid microbial composition detection device for home use of claim 1, wherein the assembly body 5 comprises an upper cover (16), a steering gear (15), a cam element (12), an expansion bracket (11) and a cotton swab clamp from top to bottom in the assembly process; the telescopic frame (11) and the upper cover (16) are fixed by corresponding clamping grooves, the telescopic frame is provided with five clamping grooves with the same size, and the cotton rod clamp is fixed in the corresponding clamping groove of the telescopic frame (11) and is connected with the center by a spring; the steering engine (15) is powered by a battery, and a corresponding circuit board and a chip are arranged in a control box above the steering engine to control the rotation speed, time and angle of the steering engine and control the steering engine through the control box; the upper cover (16) is linked with the shell through an electromagnet, and the switch of the electromagnet is controlled through the control box, so that the lifting of the electromagnet is controlled; put down assembly body 3 that is equipped with short cotton stick (1), start low-speed motor (10) in assembly body 2, detect dish (2) and begin to rotate at reasonable rotational speed at the uniform velocity.
3. The convenient liquid microorganism component detection device for home use of claims 1 and 2, wherein the assembly body (5) is required to complete the installation of the short cotton swab (1) before starting the machine, the assembly body (4) for fixing the cotton swab (cotton swab clamp) is composed of a cotton swab clamp 1(13) and a cotton swab clamp 2(14), and the cotton swab clamp 1(13) is connected with the cotton swab clamp 2(14) through a spring; firstly, opening the cotton swab clamp, inserting the short cotton swab (1) into a fixed position, and then closing the cotton swab clamp;
after the short cotton swab (1) is fixed, the assembly body 2 is moved to a corresponding position of the guide rail to be fixed.
4. The convenient liquid microorganism component detection device for home use as claimed in claims 1, 2 and 3, wherein the assembly body 5 stops the rotation of the detection disc (2) after rotating for a fixed time (the short cotton swab (1) is dipped with the liquid to be detected), pulls up the assembly body 3, and restores the magnetic force of the electromagnet through the control box; then starting a steering engine (15), and starting the cam element (12) to rotate under the driving of the steering engine (15) to form a cam mechanism, so that the rotating motion of the cam is changed into the linear intermittent and reciprocating motion of the cotton swab clamp; the cotton rod clamp is driven to move to the periphery along the clamping groove of the telescopic frame (11) through the rotation of the cam element (12), and the steering engine (15) automatically stops under the control of the chip after the cotton rod clamp moves to the end point; open the cotton swab clamp afterwards, in short cotton swab (1) falls into centrifuging tube (3) of assembly body 2 (base part) automatically, fixed centrifuging tube lid (4) and centrifuging tube (3).
5. The domestic convenient liquid microorganism component detection device according to claim 1, wherein during the assembly process of the assembly body 2, the sliding table (18) is firstly placed on the guide rail, and the buckle at the bottom of the sliding table (18) is matched with the buckle of the guide rail, so as to ensure that the sliding table (18) and the guide rail can realize relative sliding; the base and the battery (8) are arranged on the sliding table and consist of a cylinder and a base; the cylinder is a customized battery for supplying power, a chip (21) is arranged in the middle of the cylinder for controlling the whole device, and six bulges are arranged on the periphery of the cylinder on the base and used for fixing the shell (7); the shell (7) is a hollow cylinder, a large hole is formed in the middle of the bottom of the shell and used for inserting and fixing a cylinder battery, 5 slots are formed in the periphery of the large hole and used for placing 5 centrifuge tubes (3), and 6 holes in the bottom of the shell are matched with the base; the positions of the 5 centrifugal tubes (3) are matched with the 5 holes of the partition plate (9), so that the partition plate (9) can be lowered to a proper position along the centrifugal tubes (3); a low-speed motor (10) is arranged in a groove on the partition plate (9), and the power supply of the motor comes from a base and a battery (8) below; the rotary table (6) is placed on the low-speed motor (10) to ensure that the low-speed motor (10) drives the rotary table (6) to rotate at a stable speed; the detection disc (2) is placed above the rotary table (6), and the cross-shaped protrusion on the upper part of the rotary table (6) is matched with the cross-shaped groove on the bottom of the detection disc (2), so that the detection disc (2) can rotate along with the rotary table (6); the object stage (5) is arranged on the upper part of the clapboard (9) and is a cylinder with 6 holes; the middle 1 big hole is used for the detection disc (2) to pass through, and the surrounding 5 small holes are used for the 5 centrifugal tubes (3) to pass through; the object stage (5) can be placed with special 6-hole filter paper to prevent the liquid to be detected from dropping to pollute the instrument in the process of moving ahead, and the cleaning is convenient.
6. The portable liquid microbial composition detection device of claim 1, 5, wherein said medicine table (19) is composed of a base plate having 5 circular cavities, a supporting shaft and a rotatable tube cover; the medicine table can be opened by rotating the tube cover clockwise, and 5 reagent bottles (20) can be respectively placed in the medicine table; the reagent bottle contains a label, and quantitative detection liquid can be added according to specific requirements; the reagent bottle (20) is internally provided with a pressure device, and can quantitatively inject the same volume of test solution into the reagent bottle (20).
7. The domestic convenient liquid microbial component detection device according to claims 1, 5 and 6, wherein after a certain set time, the liquid to be detected and the reagent are subjected to sufficient color reaction, and a result can be obtained by comparing a color comparison card, so that preliminary judgment can be made; taking out the detection disc (2), the centrifugal tube (3) and the short cotton swab (1), properly disposing and discarding, then starting the steering engine (15) again, wherein the steering engine (15) drives the cam element (12) to rotate again, so that the cotton swab clamp gradually returns to the original position under the traction action of the spring;
the steps of the microorganism detection method include:
s1, taking down the assembly body 2, and placing the centrifugal tube (3) in a small hole of the objective table (5);
s2, injecting a liquid to be detected into the detection disc (2) and placing the liquid to be detected at a corresponding position of the assembly body 2; placing the assembly body 2 back on the guide rail, and moving the assembly body 2 until the assembly body reaches the buckle position below the medicine platform;
s3, opening the rotatable tube cover of the reagent bottle (17), adding the required medicine into the tube cover, and then covering the tube cover; the required medicine amount is set through the main board, and the control box is started to enable the medicine to flow into the centrifugal tube (3) below;
s4, opening the cotton swab clamps (13) and (14), and inserting the short cotton swab (1) into the proper position of the two cotton swab clamps;
s5, moving the assembly body 2 to a buckle position below the assembly body 5;
s6, starting a control box, descending the assembly body 5 to a proper position, and starting the rotation of the detection disc (2) to make the short cotton swab (1) be stained with the liquid to be detected; then the assembly body 5 automatically rises, and the short cotton swab (1) moves outwards to the upper part of the centrifugal tube (3);
s7, loosening the cotton swab clamps (13) and (14), and enabling the short cotton swab (1) to fall into the centrifuge tube (3) for reaction; the cotton rod clamps (13) and (14) automatically return to the original positions;
s8, comparing the color displayed by the reagent in the centrifugal tube (3) by using a colorimetric card to obtain a primary judgment;
s9, taking out the detection disc (2), the short cotton stick (1) and the centrifuge tube (3), and properly processing and discarding; and cleaning the instrument and finishing detection.
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Application publication date: 20210219 |