CN112336653A

CN112336653A - Cold algae functional toothpaste and preparation method thereof

Info

Publication number: CN112336653A
Application number: CN202011173974.1A
Authority: CN
Inventors: 艾买提·买买提明; 热依阿古丽·阿布迪卡迪尔
Original assignee: Xinjiang Xinguang Biotechnology Co ltd
Current assignee: Xinjiang Xinguang Biotechnology Co ltd
Priority date: 2020-10-28
Filing date: 2020-10-28
Publication date: 2021-02-09

Abstract

The invention relates to the technical field of toothpaste and a preparation method thereof, in particular to a cold Tianshan water alga functional toothpaste and a preparation method thereof. The preparation method is simple and convenient, and the obtained functional toothpaste containing the Tianshan cold algae not only can maintain the gum, relieve gingival bleeding and remove peculiar smell in the oral cavity, but also can prevent periodontitis, thereby realizing comprehensive health of the oral cavity.

Description

Cold algae functional toothpaste and preparation method thereof

Technical Field

The invention relates to the technical field of toothpaste and a preparation method thereof, in particular to a functional toothpaste containing Tianshan cold algae and a preparation method thereof.

Background

Such as Naeis nutritious toothpaste, which takes vitamins as active ingredients to care teeth; the Heimei active refreshing toothpaste takes an organic antibacterial agent and a plant extract as active ingredients, inhibits harmful bacteria in the oral cavity and refreshes breath, and the organic antibacterial agent and the plant extract are star toothpastes with excellent market performance.

The 'pyrenoidosa' is taken as a new high-energy nutritional food raw material, is known as 'canned sun', and contains 58% of protein, 18 amino acids, 16 vitamins (containing B12), 11 minerals, chlorophyll a and B, lutein, carbohydrates, nucleic acid, ferment, carotene, dietary fiber, unsaturated fatty acid omega-3, glycoprotein, nucleoprotein, ribonucleic acid (RNA), deoxyribonucleic acid (DNA) and the like. And the edible chlorella pyrenoidosa has strict requirements on inoculation and cultivation environments and can be realized only under the aseptic condition of GMP (good manufacturing practice) pharmaceutical grade production standard, so that the product is very safe.

The chlorella pyrenoidosa Cell (CGF) growth promoting factor has very high biological activity and excellent adsorption performance, and if functional toothpaste of chlorella pyrenoidosa nano-ultrafine powder is researched, the functional toothpaste can play a key role in treating common odontopathy such as tooth stain, dental plaque and tartar on the surface of teeth in the process of tooth brushing, so that the functional toothpaste can not only maintain the gums, but also relieve gingival bleeding; and can relieve gingival inflammation and remove oral odor by inhibiting oral harmful bacteria, thereby bringing more health to life. And research finds that reports of producing functional toothpaste by using chlorella pyrenoidosa nano-micropowder as a raw material are not seen so far.

Disclosure of Invention

The invention provides a functional toothpaste of Tianshan cold algae and a preparation method thereof, which overcome the defects of the prior art and can obtain the functional toothpaste of Tianshan cold algae which can not only maintain gum, relieve gum bleeding and remove peculiar smell in oral cavity, but also prevent periodontitis.

One of the technical schemes of the invention is realized by the following measures: a functional toothpaste of cold algae in Tianshan mountain comprises 0.5-5% of chlorella pyrenoidosa nanometer superfine powder, 15-30% of calcium carbonate, 15-25% of xylitol, 7-20% of hydrated silica, 3-15% of glycerol, 0.3-0.8% of methyl hydroxybenzoate, 0.2-1.5% of sodium lauryl sulfate, 0.1-1% of tetrasodium pyrophosphate, 0.2-1.2% of cellulose gum xanthan gum, 0.1-1% of sodium benzoate, 0.5-3% of edible essence and the balance of reverse osmosis secondary purified water.

The following is a further optimization or/and improvement of one of the above-mentioned technical solutions of the invention:

the preparation method comprises the following steps: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

The chlorella pyrenoidosa nanometer ultrafine powder is obtained according to the following method: firstly, carrying out sterile culture on chlorella pyrenoidosa cells in logarithmic growth phase, collecting the cells and crushing the cells to obtain cell lysates and unbroken cell fragments; then separating cell inner solution and unbroken cell fragments by a high-speed freezing and centrifuging technology, and breaking the unbroken cell fragments by a low-temperature continuous breaking technology to obtain the chlorella pyrenoidosa nano-micropowder.

The particle size of the chlorella pyrenoidosa nano-ultrafine powder is 50nm to 300 nm.

The stirring rate of the above-mentioned first mixing and stirring is 15rpm to 25 rpm.

The stirring speed of the second mixing, homogenizing and stirring is 1200rpm to 1500 rpm.

The second technical scheme of the invention is realized by the following measures: a preparation method of a functional toothpaste containing cold algae in Tianshan mountain comprises the following steps: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

The following is further optimization or/and improvement of the second technical scheme of the invention:

The particle size of the Chlorella pyrenoidosa nanometer superfine powder is 50nm to 300 nm.

The stirring speed of the first mixing and stirring is 15rpm to 25rpm, or/and the stirring speed of the second mixing and stirring is 1200rpm to 1500 rpm.

The preparation method is simple and convenient, and the obtained functional toothpaste containing the Tianshan cold algae not only can maintain the gum, relieve gingival bleeding and remove peculiar smell in the oral cavity, but also can prevent periodontitis, thereby realizing comprehensive health of the oral cavity.

Detailed Description

The present invention is not limited by the following examples, and specific embodiments may be determined according to the technical solutions and practical situations of the present invention. The various chemical reagents and chemicals mentioned in the present invention are all well known and commonly used in the art, unless otherwise specified.

The invention is further described below with reference to the following examples:

example 1: the functional toothpaste of the cold algae in Tianshan mountain comprises, by weight, 0.5% to 5% of chlorella pyrenoidosa nano-micropowder, 15% to 30% of calcium carbonate, 15% to 25% of xylitol, 7% to 20% of hydrated silica, 3% to 15% of glycerol, 0.3% to 0.8% of methyl hydroxybenzoate, 0.2% to 1.5% of sodium lauryl sulfate, 0.1% to 1% of tetrasodium pyrophosphate, 0.2% to 1.2% of cellulose gum xanthan gum, 0.1% to 1% of sodium benzoate, 0.5% to 3% of edible essence and the balance of reverse osmosis secondary purified water.

In the invention, the chlorella pyrenoidosa nano ultrafine powder can be used as a tooth anti-inflammatory antibacterial agent and a breath freshener, calcium carbonate can be used as an abrasive, xylitol and glycerol can be used as a humectant, sodium lauryl sulfate can be used as a foaming agent and a cleaning agent, cellulose gum xanthan gum can be used as a thickening agent, sodium benzoate and methyl hydroxybenzoate can be used as preservatives, edible essences can be selected from various food essences such as mint, wintergreen, spearmint and the like, and a sweetening agent can be properly added according to the taste, and can be selected from saccharin sodium and aspartame.

Example 2: as an optimization of the above example, the preparation method comprises the following steps: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

Example 3: as optimization of the above examples, Chlorella pyrenoidosa nano-micropowder was obtained according to the following method: firstly, carrying out sterile culture on chlorella pyrenoidosa cells in logarithmic growth phase, collecting the cells and crushing the cells to obtain cell lysates and unbroken cell fragments; then separating cell inner solution and unbroken cell fragments by a high-speed freezing and centrifuging technology, and breaking the unbroken cell fragments by a low-temperature continuous breaking technology to obtain the chlorella pyrenoidosa nano-micropowder.

The high-speed freezing centrifugation technology and the low-temperature continuous crushing technology involved in the invention are all the prior known technologies in the field.

Example 4: as an optimization of the above examples, the particle size of the Chlorella pyrenoidosa nano-micropowder is 50nm to 300nm

Example 5: as an optimization of the above embodiment, the stirring rate of the first mixing and stirring is 15rpm to 25 rpm.

Example 6: as optimization of the above embodiment, the stirring speed of the second mixing and homogenizing stirring is 1200rpm to 1500 rpm.

Example 7: the preparation method of the functional toothpaste containing the cold algae in the Tianshan mountain is carried out according to the following method: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

Example 8: the functional toothpaste containing the cold algae is prepared by the following steps: step one, dissolving 10g of mint and 10g of saccharin sodium by 120g of reverse osmosis secondary purified water to obtain essence dissolved solution for later use; secondly, adding 300g of xylitol and 130g of glycerol into 850g of reverse osmosis secondary purified water, and mixing and stirring for the first time to obtain first pulp; thirdly, under the vacuum state of-0.09 MPa, adding 20g of chlorella pyrenoidosa nano-micropowder, 350g of calcium carbonate, 5g of tetrasodium pyrophosphate, 40g of cellulose gum xanthan gum, 15g of methyl hydroxybenzoate, 10g of sodium lauryl sulfate, 140g of hydrated silica and 5g of sodium benzoate into the first pulp, carrying out mixing, homogenizing and stirring for 25 minutes for the second time, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second pulp; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 minutes under the vacuum sterile state of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

Example 9: the functional toothpaste containing the cold algae is prepared by the following steps: firstly, dissolving 10g of ilex chinensis and 10g of aspartame with 120g of reverse osmosis secondary purified water to obtain essence dissolved solution for later use; secondly, adding 300g of xylitol and 130g of glycerol into 850g of reverse osmosis secondary purified water, and mixing and stirring for the first time to obtain first pulp; thirdly, under the vacuum state of-0.09 MPa, 18g of chlorella pyrenoidosa nano-micropowder, 350g of calcium carbonate, 5g of tetrasodium pyrophosphate, 40g of cellulose gum xanthan gum, 15g of methyl hydroxybenzoate, 10g of sodium lauryl sulfate, 140g of hydrated silica and 5g of sodium benzoate are added into the first pulp, and then the first pulp is mixed, homogenized and stirred for 30 minutes for the second time, and then the second pulp is obtained by centrifugation, filter pressing, sand removal and separation; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 20 minutes under the vacuum sterile state of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

Example 10: the functional toothpaste containing the cold algae is prepared by the following steps: firstly, dissolving 10g of spearmint and 10g of saccharin sodium by 120g of reverse osmosis secondary purified water to obtain essence dissolved solution for later use; secondly, adding 300g of xylitol and 130g of glycerol into 850g of reverse osmosis secondary purified water, and mixing and stirring for the first time to obtain first pulp; thirdly, under the vacuum state of-0.09 MPa, adding 15g of chlorella pyrenoidosa nano-micropowder, 350g of calcium carbonate, 5g of tetrasodium pyrophosphate, 40g of cellulose gum xanthan gum, 15g of methyl hydroxybenzoate, 10g of sodium lauryl sulfate, 140g of hydrated silica and 5g of sodium benzoate into the first pulp, carrying out mixing, homogenizing and stirring for 35 minutes for the second time, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second pulp; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 25 minutes under the vacuum sterile state of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

In the invention, the chlorella pyrenoidosa nano-fine powder obtained by aseptic culture, collection, crushing and spray drying of chlorella pyrenoidosa nano-cells is used as a main effective component of the functional toothpaste of the invention, and the dosage in the raw material formula has close relation with the function of removing oral odor and periodontitis.

The chlorella pyrenoidosa nano-cells have the following advantages:

1. the high content of CGF in the cells has the functions of improving the oral environment, repairing damaged gingival tissue cells and preventing periodontitis and gingival bleeding;

2. the abundant alkaline polysaccharide in the cells can promote the growth and tissue repair of periodontal epithelium and prolong the retention time of the chlorella pyrenoidosa effective components on periodontal pockets and oral mucosa, thereby effectively reducing the breeding of dental plaque bacteria, improving the oral immunity and keeping the clean oral environment;

3. the high-activity potassium ions (k +) of the composition can effectively relieve dentin hypersensitivity and improve the overall comfort of the teeth of a patient;

4. the unsaturated fatty acid can effectively reduce cholesterol, triglyceride, LDL-C (low density lipoprotein cholesterol) and VLDL-C (very low density lipoprotein cholesterol) in blood plasma, prevent thrombosis, and prevent periodontal tissue nutrition deficiency.

The functional toothpaste of the cold Tianshan algae obtained in the embodiments 8 to 10, through product inspection, meets the requirements of national standards GB8327-2008 and QB/T2966 of toothpaste, and through bacteriostasis tests, has 99% of bacteriostasis rate on staphylococcus aureus, escherichia coli, candida albicans, streptococcus mutans and aspergillus niger; after oral cavity odor test, no odor is generated in the oral cavity after 9 days in average in example 8, no odor is generated in the oral cavity after 11 days in average in example 9, and no odor is generated in the oral cavity after 13 days in average in example 10; the gingivitis returned to normal after an average of 7 days in example 8, 9 days in example 9, and 13 days in example 10, as a result of the gingivitis test.

In conclusion, the preparation method is simple and convenient, and the obtained Tianshan cold water algae functional toothpaste can not only maintain the gum, relieve gingival bleeding and remove oral peculiar smell, but also prevent periodontitis, thereby realizing comprehensive health of the oral cavity.

The technical characteristics form an embodiment of the invention, which has strong adaptability and implementation effect, and unnecessary technical characteristics can be increased or decreased according to actual needs to meet the requirements of different situations.

Claims

1. The functional toothpaste is characterized by comprising, by weight, 0.5-5% of chlorella pyrenoidosa nano-micropowder, 15-30% of calcium carbonate, 15-25% of xylitol, 7-20% of hydrated silica, 3-15% of glycerol, 0.3-0.8% of methylparaben, 0.2-1.5% of sodium lauryl sulfate, 0.1-1% of tetrasodium pyrophosphate, 0.2-1.2% of cellulose gum xanthan gum, 0.1-1% of sodium benzoate, 0.5-3% of edible essence and the balance of reverse osmosis secondary purified water.

2. The functional toothpaste of the cold Tianshan algae of claim 1, which is prepared according to the following steps: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

3. The functional toothpaste of cold water algae in Tianshan mountain of claim 1 or 2, characterized in that the chlorella pyrenoidosa nano-micropowder is obtained according to the following method: firstly, carrying out sterile culture on chlorella pyrenoidosa cells in logarithmic growth phase, collecting the cells and crushing the cells to obtain cell lysates and unbroken cell fragments; then separating cell inner solution and unbroken cell fragments by a high-speed freezing and centrifuging technology, and breaking the unbroken cell fragments by a low-temperature continuous breaking technology to obtain the chlorella pyrenoidosa nano-micropowder.

4. The cold water celestial mountain algae functional toothpaste according to claim 2 or 3, wherein the particle size of the chlorella pyrenoidosa nano-micropowder is 50nm to 300 nm.

5. The cold Tianshan algal functional toothpaste according to claim 2 or 3 or 4, characterized in that the stirring rate of the first mixing stirring is 15rpm to 25 rpm.

6. The cold Tianshan water algae functional toothpaste according to claim 2, 3, 4 or 5, wherein the stirring speed of the second mixing and homogenizing stirring is 1200rpm to 1500 rpm.

7. A method for preparing the cold Tianshan algal functional toothpaste according to claim 1, characterized by comprising the following steps: firstly, dissolving a required amount of edible essence by using reverse osmosis secondary purified water to obtain an essence dissolved solution for later use; secondly, adding required amount of xylitol and glycerol into the rest reverse osmosis secondary purified water for first mixing and stirring to obtain first pulp; thirdly, adding required amounts of chlorella pyrenoidosa nano-micropowder, calcium carbonate, tetrasodium pyrophosphate, cellulose gum xanthan gum, methylparaben, sodium lauryl sulfate, hydrated silica and sodium benzoate into the first slurry under the vacuum state of-0.09 MPa, carrying out second mixing, homogenizing and stirring for 25 to 35 minutes, and then carrying out centrifugation, filter pressing, sand removal and separation to obtain a second slurry; and fourthly, drying the separated second pulp, crushing the second pulp by using a tooth type crusher, mixing the crushed second pulp with an essence dissolving liquid, and emulsifying the mixture for 15 to 25 minutes under the vacuum aseptic condition of-0.09 MPa to obtain the cold algae in Tianshan mountain functional toothpaste.

8. The method for preparing the functional toothpaste containing the cold water algae in the Tianshan mountain of claim 7, wherein the chlorella pyrenoidosa nano-micropowder is obtained by the following method: firstly, carrying out sterile culture on chlorella pyrenoidosa cells in logarithmic growth phase, collecting the cells and crushing the cells to obtain cell lysates and unbroken cell fragments; then separating cell inner solution and unbroken cell fragments by a high-speed freezing and centrifuging technology, and breaking the unbroken cell fragments by a low-temperature continuous breaking technology to obtain the chlorella pyrenoidosa nano-micropowder.

9. The method for preparing a cold water asparagus functional toothpaste in Tianshan mountain according to claim 7 or 8, characterized in that the particle size of the chlorella pyrenoidosa nano-micropowder is 50nm to 300 nm.

10. The method for preparing the cold Tianshan algal functional toothpaste according to claim 7, 8 or 9, characterized in that the stirring rate of the first mixing stirring is 15rpm to 25 rpm; or/and the stirring speed of the second mixing, homogenizing and stirring is 1200rpm to 1500 rpm.