CN112314332A - Hericium erinaceus cultivation-based jade coral inoculation method - Google Patents
Hericium erinaceus cultivation-based jade coral inoculation method Download PDFInfo
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- CN112314332A CN112314332A CN202011356489.8A CN202011356489A CN112314332A CN 112314332 A CN112314332 A CN 112314332A CN 202011356489 A CN202011356489 A CN 202011356489A CN 112314332 A CN112314332 A CN 112314332A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
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Abstract
The invention discloses a pseudopterogorgia pseudostellaria breeding method based on hericium erinaceus cultivation, which comprises the following steps of: inoculating coralloid Hericium erinaceus flowers onto a mother culture medium through tissue separation, and performing mother culture under aseptic conditions, wherein the opening of a fungus bag is sleeved on the outer wall of a bottom cylinder, so that two groups of connecting rods are close to each other, a movable plate is driven to rotate along a lower fixed plate, and the fungus bag is clamped and fixed on the bottom cylinder by two groups of clamping sleeves; then, start the material loading motor, drive the material loading screw rod and rotate, with the mixture in the blending tank along spiral material loading pipe input unloading pipe, again along connecting funnel with a end section of thick bamboo enter into the fungus bag in, then control cylinder work takes off the fungus bag from a end section of thick bamboo, then sterilizes to the fungus bag again: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain; culturing the inoculated fungus bags in the dark, and inoculating coralline Hericium erinaceus; the invention greatly improves the efficiency of the inoculation work of the coralloid hericium erinaceus.
Description
Technical Field
The invention belongs to the technical field of hericium erinaceus cultivation, and particularly relates to a pseudopterogous coralline inoculation method based on hericium erinaceus cultivation.
Background
Hericium coralloides (Hericium coralloides), also known as pine mushrooms and bearded beards, has fruit bodies with meat quality, usually comprising several soft and tough short and small main branches, each of which branches repeatedly to resemble corals, and is called Hericium coralloides.
A contrast document CN102754563B discloses a production method of coralloid Hericium erinaceus, which belongs to the field of edible fungi, and mainly comprises the steps of breeding mother seeds which are cultured for 10-12 days in the dark under the conditions of 23-27 ℃ and 40-60% of air relative humidity, expanding culture of stock seeds and cultivated seeds which are cultured for 22-28 days in the dark under the conditions of 22-28 ℃ and 40-60% of air relative humidity, cultivating and producing the coralloid Hericium erinaceus, and further comprising conditions such as fruiting management and the like. The invention also discloses a mother culture medium and a cultivation culture material. The method has the advantages of high hypha growth speed, short growth period, high fruiting body yield and high biotransformation rate of the coralloid hericium erinaceus.
In the prior art, a solid stock culture medium needs to be prepared during the inoculation process of coralloid hericium erinaceus; bagging: filling the solid stock culture medium into a fungus bag, tightly filling and compacting; the work is usually completed by manual operation, so that the inoculation efficiency of the coralloid hericium erinaceus is greatly reduced, and the preparation cost of the coralloid hericium erinaceus is increased.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, a solid stock culture medium is required to be prepared in the process of breeding coralloid hericium erinaceus; bagging: filling the solid stock culture medium into a fungus bag, tightly filling and compacting; the work is usually completed by manual operation, so that the inoculation efficiency of the coralloid hericium erinaceus is greatly reduced, the cost for preparing the coralloid hericium erinaceus is increased, and the pseudohericium coralloid inoculation method based on hericium erinaceus cultivation is provided.
The purpose of the invention can be realized by the following technical scheme:
the method for breeding the coral based on the hericium erinaceus cultivation comprises the following steps:
the first step is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 23-25 deg.C in dark for 8-10 days to obtain Hericium coralloides mother strain;
the second step is that: culturing the coralloid hericium erinaceus tree flower protospecies: the method comprises the following steps of adding the following raw materials, by weight, 150 portions of rotten wood blocks, 200 portions of soybean 150, 200 portions of corn 150, 3-5 portions of monopotassium phosphate, 3-5 portions of magnesium sulfate, 3-5 portions of peptone and 3-5 portions of agar into a feeding funnel, enabling the raw materials to fall into a crushing shell on a support along the feeding funnel, simultaneously starting a crushing motor to work, driving a rotating shaft to rotate by the crushing motor, driving an annular plate to rotate by the rotating shaft, sleeving a connecting shaft at a position, far away from the circle center, of the annular plate, arranging a plurality of groups of connecting shafts in an annular array, and uniformly arranging a plurality of groups of cutting pieces on each group of connecting shafts so that the cutting pieces cut and crush the;
the third step: the crushed raw materials enter a mixing tank through a connecting pipe, then a driving motor is started to work to drive a driving gear to rotate, the driving gear is meshed with a driven gear, and the driven gear drives a stirring rod and a stirring paddle on the stirring rod to stir and mix the crushed raw materials to obtain a mixture;
the fourth step: sleeving the opening of the fungus bag on the outer wall of the bottom cylinder, and then controlling the air cylinder to work to enable the two groups of connecting rods to be close to each other to drive the movable plate to rotate along the lower fixing plate, so that the two groups of clamping sleeves clamp and fix the fungus bag on the bottom cylinder; then, start the material loading motor, drive the material loading screw rod and rotate, with the mixture in the blending tank along spiral material loading pipe input unloading pipe, again along connecting funnel with a end section of thick bamboo enter into the fungus bag in, then control cylinder work takes off the fungus bag from a end section of thick bamboo, then sterilizes to the fungus bag again: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain;
the fifth step: culturing inoculated strain bag at 23-25 deg.C in dark for 25-30 days, and inoculating Hericium coralloides.
Preferably, the top of the support is provided with a crushing mechanism, the middle of the support is provided with a mixing mechanism, the discharge end of the mixing mechanism is connected with the feed end of the feeding mechanism, and the discharge end of the feeding mechanism is provided with a bagging mechanism.
Preferably, the crushing mechanism comprises a feeding funnel, a crushing shell and a crushing motor, the crushing shell is installed on the support, the feeding funnel is arranged at the top of the crushing shell, the crushing motor is arranged on the top surface of the support, the output end of the crushing motor is connected with a rotating shaft, the rotating shaft extends into the crushing shell and is rotatably connected with the crushing shell, three groups of annular plates are sleeved on the rotating shaft at equal intervals, connecting shafts are sleeved at positions, away from the center of a circle, of the annular plates, a plurality of groups of annular arrays of the connecting shafts are arranged, a plurality of groups of cutting blades are uniformly arranged on each group of connecting shafts, a bottom discharge port of the crushing shell is connected with a feed port of;
the crushing shell comprises a first shell, a second shell and a buckle, the second shell is positioned on the first shell, one side of the second shell is movably connected with one side of the first shell, the other side of the second shell is movably connected with the other side of the first shell, and the first shell is arranged on the support.
Preferably, the mixing mechanism comprises a mixing tank and a stirring mechanism, the mixing tank is mounted on the bracket through a mounting block, the stirring mechanism is arranged on the mixing tank, and the stirring mechanism comprises a driving motor, a driving gear, a driven gear, a stirring rod and a stirring paddle; the connecting pipe extends to in the blending tank to rotate with the blending tank and be connected, driving motor sets up on the top surface of blending tank, and driving motor's output extends to in the blending tank, and with driving gear connection, the driving gear is connected with driven gear meshing, driven gear rotates the cover and establishes on the outer wall of connecting pipe, the driven gear bottom surface is kept away from centre of a circle position department and is provided with the puddler, the puddler is the Z shape pole, and the bottom cover of puddler is equipped with the stirring rake.
Preferably, the feed mechanism includes spiral material loading pipe, material loading motor, unloading pipe, the bottom discharge mouth and the feed inlet of spiral material loading pipe of blending tank are connected, spiral material loading pipe is installed on the support, and the one end that the blending tank was kept away from to spiral material loading pipe is provided with material loading motor, material loading motor's output is provided with the material loading screw rod, the material loading screw rod rotates to be installed in spiral material loading pipe, the discharge mouth and the unloading union coupling that spiral material loading pipe is close to material loading motor one end, be provided with bagging-off mechanism on the unloading pipe.
Preferably, the bagging mechanism comprises an upper fixed plate, a connecting funnel, a lower fixed plate, a movable plate, a connecting rod, a cylinder, a clamping plate, a clamping sleeve and a bottom barrel; connect the funnel and be connected with the discharge end of unloading pipe, the discharge end of connecting the funnel is connected with end section of thick bamboo, the upper fixed plate cover is established on the outer wall of connecting the funnel, the lower fixed plate cover is established on the outer wall of end section of thick bamboo, the upper fixed plate passes through branch and installs on the outer wall of spiral feeding pipe, the upper fixed plate is located the top of lower fixed plate, the both sides difference level of end section of thick bamboo is provided with the connecting rod, the both ends of connecting rod are overlapped respectively and are equipped with the fly leaf, the connecting rod is located the middle part of fly leaf, and the top of fly leaf and the bottom surface swing joint of lower fixed plate, the bottom of fly.
Preferably, the cylinders are movably arranged between the two groups of connecting rods and symmetrically arranged along the bottom barrel.
Compared with the prior art, the invention has the beneficial effects that: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 23-25 deg.C in dark for 8-10 days to obtain Hericium coralloides mother strain;
culturing the coralloid hericium erinaceus tree flower protospecies: the method comprises the following steps of adding the following raw materials, by weight, 150 portions of rotten wood blocks, 200 portions of soybean 150, 200 portions of corn 150, 3-5 portions of monopotassium phosphate, 3-5 portions of magnesium sulfate, 3-5 portions of peptone and 3-5 portions of agar into a feeding funnel, enabling the raw materials to fall into a crushing shell on a support along the feeding funnel, simultaneously starting a crushing motor to work, driving a rotating shaft to rotate by the crushing motor, driving an annular plate to rotate by the rotating shaft, sleeving a connecting shaft at a position, far away from the circle center, of the annular plate, arranging a plurality of groups of connecting shafts in an annular array, and uniformly arranging a plurality of groups of cutting pieces on each group of connecting shafts so that the cutting pieces cut and crush the; the crushing mechanism has the advantages of simple structure, good crushing effect and high working efficiency, is mainly composed of the cutting blade on the connecting shaft, can crush the raw materials for multiple times, is convenient for mixing the raw materials, and ensures that the nutrient components of the prepared solid stock culture medium can be fully absorbed by the coralliform hericium erinaceus;
the crushed raw materials enter a mixing tank through a connecting pipe, then a driving motor is started to work to drive a driving gear to rotate, the driving gear is meshed with a driven gear, and the driven gear drives a stirring rod and a stirring paddle on the stirring rod to stir and mix the crushed raw materials to obtain a mixture; a mixing tank in the stirring mechanism is positioned under the crushing mechanism, so that the crushed raw materials directly fall into the mixing tank, a driving motor works in the mixing tank to drive a stirring paddle to rotate, so that the crushed raw materials are uniformly mixed, and the quality of the solid stock culture medium is improved;
sleeving the opening of the fungus bag on the outer wall of the bottom cylinder, and then controlling the air cylinder to work to enable the two groups of connecting rods to be close to each other to drive the movable plate to rotate along the lower fixing plate, so that the two groups of clamping sleeves clamp and fix the fungus bag on the bottom cylinder; then, start the material loading motor, drive the material loading screw rod and rotate, with the mixture in the blending tank along spiral material loading pipe input unloading pipe, again along connecting funnel with a end section of thick bamboo enter into the fungus bag in, then control cylinder work takes off the fungus bag from a end section of thick bamboo, then sterilizes to the fungus bag again: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain; the mixture can be automatically and quantitatively filled into the fungus bag by arranging the feeding mechanism, meanwhile, the air cylinder of the bagging mechanism works to realize the separation and clamping of the jacket, so that the fungus bag is clamped and fixed, the automatic bagging work is realized, the inoculation efficiency of the coralloid hericium erinaceus is greatly improved, and the crushing mechanism, the mixing mechanism, the feeding mechanism and the bagging mechanism are mutually matched, so that the automatic preparation of a solid strain culture medium is realized, and the raw materials of the solid stock culture medium can be fully and uniformly distributed, so that the coralloid hericium erinaceus grows uniformly in the later period, and the product quality is high;
culturing inoculated strain bag at 23-25 deg.C in dark for 25-30 days, and inoculating Hericium coralloides.
Drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic three-dimensional structure of the mixing mechanism of the present invention.
FIG. 3 is a side view of the mixing mechanism of the present invention.
Fig. 4 is a schematic structural view of the connection relationship between the cutting blade and the rotating shaft in the present invention.
Fig. 5 is a schematic structural diagram of the relationship between the mixing mechanism and the feeding mechanism in the present invention.
FIG. 6 is a schematic view showing the structure of the interior of the mixing tank of the present invention.
Fig. 7 is a three-dimensional structure schematic diagram of the bagging mechanism in the invention.
In the figure: 1. a support; 2. a crushing mechanism; 3. a mixing mechanism; 4. a feeding mechanism; 5. a bagging mechanism; 6. a charging hopper; 7. pulverizing the shell; 8. a grinding motor; 9. a first housing; 10. a second housing; 11. buckling; 12. a rotating shaft; 13. an annular plate; 14. a connecting shaft; 15. cutting the slices; 16. a connecting pipe; 17. a mixing tank; 18. mounting blocks; 19. a spiral feeding pipe; 20. a feeding motor; 21. a discharging pipe; 22. an upper fixing plate; 23. connecting a funnel; 24. a lower fixing plate; 25. a movable plate; 26. a connecting rod; 27. a cylinder; 28. a splint; 29. a jacket; 30. a bottom cylinder; 31. a drive motor; 32. a driving gear; 33. a driven gear; 34. a stirring rod; 35. and (4) a stirring paddle.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-7, the inoculation method of pseudopterogous corals based on hericium erinaceus cultivation includes the following steps:
the first step is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 23 deg.C in dark for 8 days to obtain Hericium coralloides mother strain;
the second step is that: culturing the coralloid hericium erinaceus tree flower protospecies: adding 150 parts of rotten wood blocks, 150 parts of soybeans, 150 parts of corns, 3 parts of monopotassium phosphate, 3 parts of magnesium sulfate, 3 parts of peptone and 3 parts of agar into a feeding funnel 6, dropping the materials into a crushing shell 7 on a support 1 along the feeding funnel 6, starting a crushing motor 8 to work, driving a rotating shaft 12 to rotate by the crushing motor 8, driving an annular plate 13 to rotate by the rotating shaft 12, sleeving a connecting shaft 14 at a position of the annular plate 13 far away from the circle center, arranging a plurality of groups of connecting shafts 14 in an annular array manner, and uniformly arranging a plurality of groups of cutting pieces 15 on each group of connecting shafts 14 so that the cutting pieces 15 cut and crush the materials;
the third step: the crushed raw materials enter the mixing tank 17 through the connecting pipe 16, then, the driving motor 31 is started to work to drive the driving gear 32 to rotate, the driving gear 32 is meshed with the driven gear 33, and the driven gear 33 drives the stirring rod 34 and the stirring paddle 35 on the stirring rod 34, so that the crushed raw materials are stirred and mixed to obtain a mixture;
the fourth step: sleeving the opening of the fungus bag on the outer wall of the bottom barrel 30, and then controlling the air cylinder 27 to work, so that the two groups of connecting rods 26 are close to each other to drive the movable plate 25 to rotate along the lower fixing plate 24, so that the two groups of clamping sleeves 29 clamp and fix the fungus bag on the bottom barrel 30; then, start material loading motor 20, drive the material loading screw rod and rotate, import the mixture in blending tank 17 into unloading pipe 21 along spiral material loading pipe 19, enter into the fungus bag along connecting funnel 23 and end section of thick bamboo 30 again in, then control cylinder 27 work, take off the fungus bag from end section of thick bamboo 30, then disinfect to the fungus bag: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain;
the fifth step: culturing the inoculated strain bags at 23 deg.C in dark for 25 days, and inoculating Hericium coralloides.
The top of support 1 is provided with rubbing crusher structure 2, and the middle part of support 1 is provided with mixing mechanism 3, and mixing mechanism 3's discharge end is connected with the feed end of feed mechanism 4, is provided with bagging-off mechanism 5 on the discharge end of feed mechanism 4.
The crushing mechanism 2 comprises a feeding funnel 6, a crushing shell 7 and a crushing motor 8, wherein the crushing shell 7 is installed on a support 1, the feeding funnel 6 is arranged at the top of the crushing shell 7, the crushing motor 8 is arranged on the top surface of the support 1, the output end of the crushing motor 8 is connected with a rotating shaft 12, the rotating shaft 12 extends into the crushing shell 7 and is rotatably connected with the crushing shell 7, three groups of annular plates 13 are sleeved on the rotating shaft 12 at equal intervals, connecting shafts 14 are sleeved at positions, far away from the circle center, of the annular plates 13, a plurality of groups of annular arrays are arranged on the connecting shafts 14, a plurality of groups of cutting pieces 15 are uniformly arranged on each group of connecting shafts 14, a discharge port at the bottom of the crushing shell 7 is connected with a feed port of the connecting;
the crushing shell 7 comprises a first shell 9, a second shell 10 and a buckle 11, the second shell 10 is positioned on the first shell 9, one side of the second shell 10 is movably connected with one side of the first shell 9, the other side of the second shell 10 is movably connected with the other side of the first shell 9, and the first shell 9 is installed on the support 1.
The mixing mechanism 3 comprises a mixing tank 17 and a stirring mechanism, the mixing tank 17 is mounted on the support 1 through a mounting block 18, the mixing tank 17 is provided with the stirring mechanism, and the stirring mechanism comprises a driving motor 31, a driving gear 32, a driven gear 33, a stirring rod 34 and a stirring paddle 35; connecting pipe 16 extends to in the blending tank 17, and rotate with blending tank 17 and be connected, driving motor 31 sets up on the top surface of blending tank 17, and driving motor 31's output extends to in the blending tank 17, and be connected with driving gear 32, driving gear 32 is connected with driven gear 33 meshing, driven gear 33 rotates the cover and establishes on the outer wall of connecting pipe 16, driven gear 33 bottom surface is kept away from centre of a circle position department and is provided with puddler 34, puddler 34 is the Z shape pole, and the bottom cover of puddler 34 is equipped with stirring rake 35.
Feed mechanism 4 includes spiral material loading pipe 19, material loading motor 20, unloading pipe 21, the bottom discharge gate of blending tank 17 is connected with the feed inlet of spiral material loading pipe 19, spiral material loading pipe 19 is installed on support 1, and the one end that mixing tank 17 was kept away from to spiral material loading pipe 19 is provided with material loading motor 20, the output of material loading motor 20 is provided with the material loading screw rod, the material loading screw rod rotates and installs in spiral material loading pipe 19, spiral material loading pipe 19 is close to the discharge gate and the unloading pipe 21 of material loading motor 20 one end and is connected, be provided with bagging-off mechanism 5 on the unloading pipe 21.
The bagging mechanism 5 comprises an upper fixing plate 22, a connecting funnel 23, a lower fixing plate 24, a movable plate 25, a connecting rod 26, a cylinder 27, a clamping plate 28, a clamping sleeve 29 and a bottom barrel 30; connecting funnel 23 is connected with the discharge end of unloading pipe 21, the discharge end of connecting funnel 23 is connected with end section of thick bamboo 30, upper fixed plate 22 cover is established on connecting funnel 23's outer wall, lower fixed plate 24 cover is established on end section of thick bamboo 30's outer wall, upper fixed plate 22 passes through branch and installs on spiral feeding pipe 19's outer wall, upper fixed plate 22 is located the top of lower fixed plate 24, end section of thick bamboo 30's both sides level respectively are provided with connecting rod 26, the both ends of connecting rod 26 are overlapped respectively and are equipped with fly leaf 25, connecting rod 26 is located the middle part of fly leaf 25, and the top of fly leaf 25 and the bottom surface swing joint of lower fixed plate 24, the bottom of fly leaf 25 is provided with splint 28, be provided with between the splint 28 and press from.
Example 2
The difference from the embodiment 1 is that: the first step is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 24 deg.C in dark for 9 days to obtain Hericium coralloides mother strain;
the second step is that: culturing the coralloid hericium erinaceus tree flower protospecies: adding 175 parts of rotten wood blocks, 175 parts of soybeans, 175 parts of corns, 4 parts of monopotassium phosphate, 4 parts of magnesium sulfate, 4 parts of peptone and 4 parts of agar into a feeding funnel 6, dropping the materials into a crushing shell 7 on a support 1 along the feeding funnel 6, starting a crushing motor 8 to work, driving a rotating shaft 12 to rotate by the crushing motor 8, driving an annular plate 13 to rotate by the rotating shaft 12, sleeving a connecting shaft 14 at a position of the annular plate 13 far away from the circle center, arranging a plurality of groups of connecting shafts 14 in an annular array manner, and uniformly arranging a plurality of groups of cutting pieces 15 on each group of connecting shafts 14 so that the cutting pieces 15 cut and crush the materials;
the fifth step: culturing the inoculated strain bags at 24 deg.C in dark for 28 days, and inoculating Hericium coralloides.
Example 3
The difference from the embodiment 1 is that: the first step is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 25 deg.C in dark for 10 days to obtain Hericium coralloides mother strain;
the second step is that: culturing the coralloid hericium erinaceus tree flower protospecies: the method comprises the following steps of adding 200 parts by weight of rotten wood blocks, 200 parts by weight of soybeans, 200 parts by weight of corns, 5 parts by weight of monopotassium phosphate, 5 parts by weight of magnesium sulfate, 5 parts by weight of peptone and 5 parts by weight of agar into a feeding funnel 6, dropping the materials into a crushing shell 7 on a support 1 along the feeding funnel 6, simultaneously starting a crushing motor 8 to work, driving a rotating shaft 12 to rotate by the crushing motor 8, driving an annular plate 13 to rotate by the rotating shaft 12, sleeving a connecting shaft 14 at a position of the annular plate 13 far away from the circle center, arranging a plurality of groups of connecting shafts 14 in an annular array mode, and uniformly arranging a plurality of groups of cutting pieces 15 on each group of connecting shafts 14 so that;
the fifth step: culturing the inoculated fungus bags at 25 deg.C in dark for 30 days, and inoculating Hericium coralloides.
The working principle of the invention is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 23-25 deg.C in dark for 8-10 days to obtain Hericium coralloides mother strain;
culturing the coralloid hericium erinaceus tree flower protospecies: the method comprises the following steps of adding the following raw materials, by weight, 150 portions of rotten wood blocks, 200 portions of soybean 150, 200 portions of corn 150, 3-5 portions of monopotassium phosphate, 3-5 portions of magnesium sulfate, 3-5 portions of peptone and 3-5 portions of agar into a feeding funnel 6, enabling the raw materials to fall into a crushing shell 7 on a support 1 along the feeding funnel 6, simultaneously starting a crushing motor 8 to work, driving a rotating shaft 12 to rotate by the crushing motor 8, driving an annular plate 13 to rotate by the rotating shaft 12, sleeving a connecting shaft 14 at a position, far away from the circle center, of the annular plate 13, arranging a plurality of groups of connecting shafts 14 in an annular array mode, and uniformly arranging a plurality of groups of cutting pieces 15 on each group of connecting shafts 14 to enable the cutting pieces 15 to cut and crush; the crushing mechanism 2 has the advantages of simple structure, good crushing effect and high working efficiency, is mainly composed of the cutting blades 15 on the connecting shaft 14, can crush the raw materials for multiple times, is convenient for mixing the raw materials, and ensures that the nutrient components of the prepared solid stock culture medium can be fully absorbed by coralliform hericium erinaceus;
the crushed raw materials enter the mixing tank 17 through the connecting pipe 16, then, the driving motor 31 is started to work to drive the driving gear 32 to rotate, the driving gear 32 is meshed with the driven gear 33, and the driven gear 33 drives the stirring rod 34 and the stirring paddle 35 on the stirring rod 34, so that the crushed raw materials are stirred and mixed to obtain a mixture; a mixing tank 17 in the stirring mechanism is positioned under the crushing mechanism 2, so that the crushed raw materials directly fall into the mixing tank 17, a driving motor 31 works in the mixing tank 17 to drive a stirring paddle 35 to rotate, so that the crushed raw materials are uniformly mixed, and the quality of the solid original seed culture medium is improved;
sleeving the opening of the fungus bag on the outer wall of the bottom barrel 30, and then controlling the air cylinder 27 to work, so that the two groups of connecting rods 26 are close to each other to drive the movable plate 25 to rotate along the lower fixing plate 24, so that the two groups of clamping sleeves 29 clamp and fix the fungus bag on the bottom barrel 30; then, start material loading motor 20, drive the material loading screw rod and rotate, import the mixture in blending tank 17 into unloading pipe 21 along spiral material loading pipe 19, enter into the fungus bag along connecting funnel 23 and end section of thick bamboo 30 again in, then control cylinder 27 work, take off the fungus bag from end section of thick bamboo 30, then disinfect to the fungus bag: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain; the mixture can be automatically and quantitatively filled into the fungus bags through the arrangement of the feeding mechanism 4, meanwhile, the air cylinder 27 of the bagging mechanism 5 works to realize separation and clamping of the jacket 29, so that the fungus bags are clamped and fixed, automatic bagging is realized, the inoculation efficiency of the coralliform fungus is greatly improved, and the crushing mechanism 2, the mixing mechanism 3, the feeding mechanism 4 and the bagging mechanism 5 are mutually matched, so that the automatic preparation of the solid strain culture medium is realized, and the raw materials of the solid original strain culture medium can be fully and uniformly distributed, so that the later-stage coralliform fungus grows uniformly and the product quality is high;
culturing inoculated strain bag at 23-25 deg.C in dark for 25-30 days, and inoculating Hericium coralloides.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (7)
1. The pseudopterogorgia inoculating method based on hericium erinaceus cultivation is characterized by comprising the following steps of: the method comprises the following steps:
the first step is as follows: inoculating Hericium coralloides tree flower onto mother culture medium by tissue isolation, and culturing under aseptic condition: culturing at 23-25 deg.C in dark for 8-10 days to obtain Hericium coralloides mother strain;
the second step is that: culturing the coralloid hericium erinaceus tree flower protospecies: the method comprises the following steps of adding, by weight, 200 parts of rotten wood blocks 150, 200 parts of soybeans 150, 200 parts of corn 150, 3-5 parts of monopotassium phosphate, 3-5 parts of magnesium sulfate, 3-5 parts of peptone and 3-5 parts of agar into a feeding funnel (6), enabling the materials to fall into a crushing shell (7) on a support (1) along the feeding funnel (6), simultaneously starting a crushing motor (8) to work, driving a rotating shaft (12) to rotate by the crushing motor (8), driving an annular plate (13) to rotate by the rotating shaft (12), sleeving a connecting shaft (14) at a position, far away from the center of a circle, of the annular plate (13), arranging a plurality of groups of connecting shafts (14) in an annular array manner, and uniformly arranging a plurality of groups of cutting pieces (15) on each group of connecting shafts (14) to enable the cutting pieces (15) to cut and crush the materials;
the third step: the method comprises the following steps that the crushed raw materials enter a mixing tank (17) through a connecting pipe (16), then a driving motor (31) is started to work to drive a driving gear (32) to rotate, the driving gear (32) is meshed with a driven gear (33), the driven gear (33) drives a stirring rod (34) and a stirring paddle (35) on the stirring rod (34), and the crushed raw materials are stirred and mixed to obtain a mixture;
the fourth step: the opening of the fungus bag is sleeved on the outer wall of the bottom cylinder (30), then, the air cylinder (27) is controlled to work, so that the two groups of connecting rods (26) are close to each other, the movable plate (25) is driven to rotate along the lower fixing plate (24), and the fungus bag is clamped and fixed on the bottom cylinder (30) by the two groups of clamping sleeves (29); then, start material loading motor (20), drive the material loading screw rod and rotate, with mixture in blending tank (17) along spiral material loading pipe (19) input unloading pipe (21), again along connecting funnel (23) and end section of thick bamboo (30) enter into the fungus bag in, then control cylinder (27) work, take off the fungus bag from end section of thick bamboo (30), then disinfect to the fungus bag: sterilizing, inoculating into mother strain under aseptic condition to obtain Hericium coralloides mother strain;
the fifth step: culturing inoculated strain bag at 23-25 deg.C in dark for 25-30 days, and inoculating Hericium coralloides.
2. The jade coral inoculation method based on hericium erinaceus cultivation as claimed in claim 1, wherein the top of the support (1) is provided with the crushing mechanism (2), the middle of the support (1) is provided with the mixing mechanism (3), the discharge end of the mixing mechanism (3) is connected with the feed end of the feeding mechanism (4), and the discharge end of the feeding mechanism (4) is provided with the bagging mechanism (5).
3. The method for inoculating the coral sand based on the hericium erinaceus cultivation as claimed in claim 2, wherein the crushing mechanism (2) comprises a feeding funnel (6), a crushing shell (7) and a crushing motor (8), the crushing shell (7) is mounted on the support (1), the feeding funnel (6) is arranged at the top of the crushing shell (7), the crushing motor (8) is arranged on the top surface of the support (1), the output end of the crushing motor (8) is connected with a rotating shaft (12), the rotating shaft (12) extends into the crushing shell (7) and is rotatably connected with the crushing shell (7), three groups of annular plates (13) are sleeved on the rotating shaft (12) at equal intervals, connecting shafts (14) are sleeved at positions of the annular plates (13) far away from the center of a circle, a plurality of groups of annular arrays of the connecting shafts (14) are arranged, a plurality of groups of cutting blades (15) are uniformly arranged on each group of, a discharge port at the bottom of the crushing shell (7) is connected with a feed port of a connecting pipe (16), and a discharge port of the connecting pipe (16) extends to the mixing mechanism (3) for connection;
the crushing shell (7) comprises a first shell (9), a second shell (10) and a buckle (11), the second shell (10) is located on the first shell (9), one side of the second shell (10) is movably connected with one side of the first shell (9), the other side of the second shell (10) is movably connected with the other side of the first shell (9), and the first shell (9) is installed on the support (1).
4. The pseudopterogous coral inoculation method based on hericium erinaceus cultivation as claimed in claim 2, wherein the mixing mechanism (3) comprises a mixing tank (17) and a stirring mechanism, the mixing tank (17) is mounted on the support (1) through a mounting block (18), the stirring mechanism is arranged on the mixing tank (17), and the stirring mechanism comprises a driving motor (31), a driving gear (32), a driven gear (33), a stirring rod (34) and a stirring paddle (35); connecting pipe (16) extend to in blending tank (17), and rotate with blending tank (17) and be connected, driving motor (31) set up on the top surface of blending tank (17), and the output of driving motor (31) extends to in blending tank (17), and be connected with driving gear (32), driving gear (32) are connected with driven gear (33) meshing, driven gear (33) rotate the cover and establish on the outer wall of connecting pipe (16), driven gear (33) bottom surface is kept away from centre of a circle position department and is provided with puddler (34), puddler (34) are Z shape pole, and the bottom cover of puddler (34) is equipped with stirring rake (35).
5. The jade coral inoculation method based on hericium erinaceus cultivation as claimed in claim 2, wherein the feeding mechanism (4) comprises a spiral feeding pipe (19), a feeding motor (20) and a discharging pipe (21), a bottom discharging port of the mixing tank (17) is connected with a feeding port of the spiral feeding pipe (19), the spiral feeding pipe (19) is mounted on the support (1), the feeding motor (20) is arranged at one end, away from the mixing tank (17), of the spiral feeding pipe (19), a feeding screw is arranged at an output end of the feeding motor (20), the feeding screw is rotatably mounted in the spiral feeding pipe (19), a discharging port of the spiral feeding pipe (19), which is close to one end of the feeding motor (20), is connected with the discharging pipe (21), and a bagging mechanism (5) is arranged on the discharging pipe (21).
6. The pseudopterogous coral inoculation method based on hericium erinaceus cultivation as claimed in claim 2, wherein the bagging mechanism (5) comprises an upper fixing plate (22), a connecting funnel (23), a lower fixing plate (24), a movable plate (25), a connecting rod (26), a cylinder (27), a clamping plate (28), a clamping sleeve (29), and a bottom barrel (30); the connecting funnel (23) is connected with the discharge end of the blanking tube (21), the discharge end of the connecting funnel (23) is connected with the bottom barrel (30), the upper fixing plate (22) is sleeved on the outer wall of the connecting funnel (23), the lower fixing plate (24) is sleeved on the outer wall of the bottom barrel (30), the upper fixing plate (22) is installed on the outer wall of the spiral feeding tube (19) through a support rod, the upper fixing plate (22) is positioned above the lower fixing plate (24), two sides of the bottom barrel (30) are respectively and horizontally provided with a connecting rod (26), two ends of the connecting rod (26) are respectively sleeved with a movable plate (25), and the connecting rod (26) is positioned in the middle of the movable plate (25), the top end of the movable plate (25) is movably connected with the bottom surface of the lower fixing plate (24), clamping plates (28) are arranged at the bottom end of the movable plate (25), a clamping sleeve (29) is arranged between the clamping plates (28), and the clamping sleeve (29) is matched with the outer wall of the bottom barrel (30).
7. The method for inoculating the coral sand on the basis of the Hericium erinaceus cultivation as claimed in claim 6, wherein air cylinders (27) are movably arranged between the two groups of connecting rods (26), and the air cylinders (27) are symmetrically arranged along the bottom cylinder (30).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011356489.8A CN112314332A (en) | 2020-11-26 | 2020-11-26 | Hericium erinaceus cultivation-based jade coral inoculation method |
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| CN202011356489.8A CN112314332A (en) | 2020-11-26 | 2020-11-26 | Hericium erinaceus cultivation-based jade coral inoculation method |
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| CN112314332A true CN112314332A (en) | 2021-02-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114771947A (en) * | 2022-04-30 | 2022-07-22 | 镇江崟鑫新型建材科技有限公司 | Manufacturing and bagging integrated system for premixed masonry sand and using method thereof |
-
2020
- 2020-11-26 CN CN202011356489.8A patent/CN112314332A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114771947A (en) * | 2022-04-30 | 2022-07-22 | 镇江崟鑫新型建材科技有限公司 | Manufacturing and bagging integrated system for premixed masonry sand and using method thereof |
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