CN112300286A - 抗ctla4-抗pd-1双特异性抗体及其用途 - Google Patents

抗ctla4-抗pd-1双特异性抗体及其用途 Download PDF

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CN112300286A
CN112300286A CN202010757322.6A CN202010757322A CN112300286A CN 112300286 A CN112300286 A CN 112300286A CN 202010757322 A CN202010757322 A CN 202010757322A CN 112300286 A CN112300286 A CN 112300286A
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seq
amino acid
variable region
chain variable
acid sequence
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夏瑜
王忠民
张鹏
李百勇
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Akeso Pharmaceuticals Inc
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Akeso Pharmaceuticals Inc
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CN202010757322.6A 2019-08-02 2020-07-31 抗ctla4-抗pd-1双特异性抗体及其用途 Pending CN112300286A (zh)

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AU (1) AU2020325350A1 (https=)
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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022188867A1 (zh) * 2021-03-12 2022-09-15 中山康方生物医药有限公司 提高含有免疫球蛋白Fc片段的药物的安全性的方法
WO2022188832A1 (zh) * 2021-03-12 2022-09-15 中山康方生物医药有限公司 含有抗pd-1-抗vegfa双特异性抗体的药物组合及其用途
CN115192718A (zh) * 2021-04-14 2022-10-18 中山康方生物医药有限公司 抗cd47的单克隆抗体及其用途
CN115197325A (zh) * 2021-04-14 2022-10-18 康方药业有限公司 抗体在抗肿瘤治疗中的用途
WO2023001303A1 (zh) 2021-07-23 2023-01-26 中山康方生物医药有限公司 药物组合物及用途
WO2024051223A1 (zh) 2022-09-09 2024-03-14 中山康方生物医药有限公司 药物组合及用途
WO2024141014A1 (zh) * 2022-12-30 2024-07-04 康方药业有限公司 Cd74的医药用途
WO2024183791A1 (zh) * 2023-03-07 2024-09-12 康方药业有限公司 包含抗ctla4-抗pd-1双特异性抗体的药物组合及其用途
WO2024199435A1 (zh) * 2023-03-31 2024-10-03 上海细胞治疗集团股份有限公司 双特异性抗体及自分泌双特异性抗体的工程化免疫细胞
WO2024212988A1 (zh) * 2023-04-10 2024-10-17 康方药业有限公司 抗ctla4-抗pd-1双特异性抗体的医药用途
US12195527B2 (en) 2018-08-30 2025-01-14 Akeso Biopharma, Inc. Anti-PD-1/VEGFA bifunctional antibody, pharmaceutical composition thereof
WO2025157267A1 (zh) * 2024-01-25 2025-07-31 中山康方生物医药有限公司 抗pd-1-抗vegfa的双特异性抗体的医药用途
WO2025201450A1 (zh) * 2024-03-27 2025-10-02 康方汇科(上海)生物有限公司 药物组合及用途
WO2025232896A1 (zh) * 2024-05-10 2025-11-13 康方汇科(上海)生物有限公司 药物组合及用途
CN120965861A (zh) * 2025-10-22 2025-11-18 杭州博茵生物技术有限公司 一种Fc效应消除的新型骨架流式抗体及其制备方法与应用

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106967172B (zh) 2016-08-23 2019-01-08 康方药业有限公司 抗ctla4-抗pd-1 双功能抗体、其药物组合物及其用途
AU2020324185A1 (en) * 2019-08-02 2022-03-03 CTTQ-Akeso (ShangHai) Biomed. Tech. Co., Ltd. Anti-PD-1 antibody and medical use thereof
EP4114464A4 (en) * 2020-03-05 2024-05-01 Merck Sharp & Dohme LLC Methods for treating cancer using a combination of a pd-1 antagonist, a ctla4 antagonist, and lenvatinib or a pharmaceutically acceptable salt thereof
KR20240153590A (ko) * 2022-02-24 2024-10-23 아케소 파마슈티컬스, 인코포레이티드 항-ctla4-항-pd-1 이중특이적 항체 및 치아우라닙을 포함하는 약제학적 조성물
WO2023170549A1 (en) * 2022-03-07 2023-09-14 Medimmune Llc Treatment methods using ctla-4 and pd-1 bispecific antibodies
CN118946366A (zh) * 2022-04-13 2024-11-12 齐鲁制药有限公司 包含抗ctla4和抗pd1的混合抗体的药物组合物及其治疗用途
JP2025530041A (ja) * 2022-09-09 2025-09-11 中山康方生物医▲藥▼有限公司 治療用組合せ物とその使用
WO2024092031A1 (en) 2022-10-25 2024-05-02 Seismic Therapeutic, Inc. VARIANT IgG FC POLYPEPTIDES AND USES THEREOF

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100055102A1 (en) * 2008-08-25 2010-03-04 Solomon Langermann Compositions of pd-1 antagonists and methods of use
CN106699891A (zh) * 2017-01-25 2017-05-24 北京天广实生物技术股份有限公司 一种抗pd‑l1 抗体、其药物组合物及其用途
CN106967172A (zh) * 2016-08-23 2017-07-21 中山康方生物医药有限公司 抗ctla4‑抗pd‑1 双功能抗体、其药物组合物及其用途
CN107151269A (zh) * 2016-03-04 2017-09-12 四川科伦博泰生物医药股份有限公司 一种pdl‑1抗体、其药物组合物及其用途
CN109762068A (zh) * 2018-08-09 2019-05-17 源道隆(苏州)医学科技有限公司 一种可靶向ctla4和pd-1的单基因双特异性抗体及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE60137273D1 (de) 2000-10-20 2009-02-12 Eisai R&D Man Co Ltd Verfahren zur Herstellung von 4-Phenoxy chinolin Derivaten
PL1698623T3 (pl) 2003-12-25 2015-08-31 Eisai R&D Man Co Ltd Postać krystaliczna soli 4-(3-chloro-4-(cyklopropyloaminokarbonylo)aminofenoksy)-7-metoksy-6-chinolinokarboksamidu lub jej solwatu i sposoby ich wytwarzania
CN103172731A (zh) * 2004-07-15 2013-06-26 赞科股份有限公司 优化的Fc变体
US8148532B2 (en) 2007-03-14 2012-04-03 Guoqing Paul Chen Spiro substituted compounds as angiogenesis inhibitors
ES2751549T3 (es) * 2009-11-30 2020-04-01 Janssen Biotech Inc Mutantes de anticuerpos Fc con funciones efectoras ablacionadas
CN102958942A (zh) * 2009-12-29 2013-03-06 新兴产品开发西雅图有限公司 异二聚体结合蛋白及其应用
CN107207594B (zh) * 2014-12-23 2019-05-07 百时美施贵宝公司 针对tigit的抗体
KR102799807B1 (ko) * 2015-12-30 2025-04-24 코디악 사이언시스 인코포레이티드 항체 및 이의 접합체
KR102878970B1 (ko) * 2016-08-03 2025-10-30 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 항-SIRPalpha 항체 요법의 대식세포 증진 효능에서 Fc 수용체 참여의 붕괴
WO2018035710A1 (en) * 2016-08-23 2018-03-01 Akeso Biopharma, Inc. Anti-ctla4 antibodies
CN106977602B (zh) * 2016-08-23 2018-09-25 中山康方生物医药有限公司 一种抗pd1单克隆抗体、其药物组合物及其用途
CN118027200A (zh) * 2016-12-09 2024-05-14 艾利妥 抗SIRPα抗体及其使用方法
EP3634485A4 (en) * 2017-06-07 2021-07-21 Silverback Therapeutics, Inc. CONJUGATES OF ANTIBODIES CONTAINING IMMUNOMODULATOR COMPOUNDS AND THEIR USES

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100055102A1 (en) * 2008-08-25 2010-03-04 Solomon Langermann Compositions of pd-1 antagonists and methods of use
CN107151269A (zh) * 2016-03-04 2017-09-12 四川科伦博泰生物医药股份有限公司 一种pdl‑1抗体、其药物组合物及其用途
CN106967172A (zh) * 2016-08-23 2017-07-21 中山康方生物医药有限公司 抗ctla4‑抗pd‑1 双功能抗体、其药物组合物及其用途
CN106699891A (zh) * 2017-01-25 2017-05-24 北京天广实生物技术股份有限公司 一种抗pd‑l1 抗体、其药物组合物及其用途
CN109762068A (zh) * 2018-08-09 2019-05-17 源道隆(苏州)医学科技有限公司 一种可靶向ctla4和pd-1的单基因双特异性抗体及其应用

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12195527B2 (en) 2018-08-30 2025-01-14 Akeso Biopharma, Inc. Anti-PD-1/VEGFA bifunctional antibody, pharmaceutical composition thereof
CN115073588B (zh) * 2021-03-12 2024-06-18 中山康方生物医药有限公司 提高含有免疫球蛋白Fc片段的药物的安全性的方法
WO2022188832A1 (zh) * 2021-03-12 2022-09-15 中山康方生物医药有限公司 含有抗pd-1-抗vegfa双特异性抗体的药物组合及其用途
WO2022188867A1 (zh) * 2021-03-12 2022-09-15 中山康方生物医药有限公司 提高含有免疫球蛋白Fc片段的药物的安全性的方法
CN115073588A (zh) * 2021-03-12 2022-09-20 中山康方生物医药有限公司 提高含有免疫球蛋白Fc片段的药物的安全性的方法
CN115192718A (zh) * 2021-04-14 2022-10-18 中山康方生物医药有限公司 抗cd47的单克隆抗体及其用途
WO2022218384A1 (zh) * 2021-04-14 2022-10-20 中山康方生物医药有限公司 抗cd47的单克隆抗体及其用途
WO2022218383A1 (zh) * 2021-04-14 2022-10-20 康方药业有限公司 抗体在抗肿瘤治疗中的用途
CN115197325A (zh) * 2021-04-14 2022-10-18 康方药业有限公司 抗体在抗肿瘤治疗中的用途
WO2023001303A1 (zh) 2021-07-23 2023-01-26 中山康方生物医药有限公司 药物组合物及用途
CN116059341A (zh) * 2021-07-23 2023-05-05 中山康方生物医药有限公司 药物组合物及用途
WO2024051223A1 (zh) 2022-09-09 2024-03-14 中山康方生物医药有限公司 药物组合及用途
WO2024141014A1 (zh) * 2022-12-30 2024-07-04 康方药业有限公司 Cd74的医药用途
WO2024183791A1 (zh) * 2023-03-07 2024-09-12 康方药业有限公司 包含抗ctla4-抗pd-1双特异性抗体的药物组合及其用途
WO2024199435A1 (zh) * 2023-03-31 2024-10-03 上海细胞治疗集团股份有限公司 双特异性抗体及自分泌双特异性抗体的工程化免疫细胞
WO2024212988A1 (zh) * 2023-04-10 2024-10-17 康方药业有限公司 抗ctla4-抗pd-1双特异性抗体的医药用途
WO2025157267A1 (zh) * 2024-01-25 2025-07-31 中山康方生物医药有限公司 抗pd-1-抗vegfa的双特异性抗体的医药用途
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