CN112264200A

CN112264200A - Marine invertebrate cell culture medium

Info

Publication number: CN112264200A
Application number: CN202011055865.XA
Authority: CN
Inventors: 王艳超
Original assignee: Beijing Yunfeng Shenghe Science And Trade Co ltd
Current assignee: Beijing Yunfeng Shenghe Science And Trade Co ltd
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2021-01-26

Abstract

The invention discloses a marine invertebrate cell culture medium, which comprises a base, a machine body, a top cover and a control panel, wherein the top surface of the base is fixedly connected with the bottom surface of the machine body, the bottom surface of the top cover is hinged with the top surface of the machine body, the control panel is embedded in the front surface of the machine body, the machine body comprises an inner wall, a power supply, a temperature plate, a liquid bin, a humidity plate and a culture centrifuge, the power supply is embedded in the right side of the inner wall, through a sealing structure arranged in the sealing cover, in the process of installing the sealing cover, a lower pressing plate is pushed by the force of screw thread rotation through the sealing structure, so that the lower pressing plate is attached to the liquid level, the liquid in a culture chamber is kept in a full state, the phenomenon that a large amount of bubbles are generated in the centrifugal process to influence the mixing effect is avoided, meanwhile, the lower pressing plate is firstly separated from the, reduce the pollution risk and keep the purity of the sampling.

Description

Marine invertebrate cell culture medium

Technical Field

The invention relates to the field of animal and plant cell culture devices, in particular to a marine invertebrate cell culture medium.

Background

The cell culture refers to a method for simulating in vivo environment in vitro to enable the in vivo environment to survive, grow, reproduce and maintain main structure and function, various required cells can be obtained through cell culture technology, signal transduction, cell anabolism, cell growth and proliferation and the like of the cells are researched, the conditions of nutrient components, humidity, illumination and the like of a culture medium are very strict because the cells are very fragile in vitro environment, after the culture is finished, the cells are required to be added into the culture medium, and are subjected to centrifugal treatment to obtain a layered supernatant, however, in the treatment process, whether a subpackaging tube is filled or not needs to be very careful, the subpackaging tube is not filled, the situation that the subpackaging tube is not filled frequently occurs, in the situation, the centrifugal treatment easily generates a large amount of bubbles, the mixing effect is poor, and meanwhile, when sampling is started, the internal solution is partially dragged out to contact with the outside due to the bubbles, may cause contamination and affect the purity of the sample.

Disclosure of Invention

In view of the above problems, the present invention provides a marine invertebrate cell culture medium.

In order to achieve the purpose, the invention is realized by the following technical scheme: a marine invertebrate cell culture medium structurally comprises a base, a machine body, a top cover and a control panel, wherein the top surface of the base is fixedly connected with the bottom surface of the machine body, the bottom surface of the top cover is hinged with the top surface of the machine body, and the control panel is embedded in the front surface of the machine body; the organism includes inner wall, power, temperature board, liquid storehouse, humidity board, cultivates centrifuge, the power imbeds in the inner wall right side, temperature board bottom surface fixed mounting is in the inner wall top surface, liquid storehouse and inner wall left side integrated into one piece, humidity board bottom surface fixed mounting is in the inner wall top surface, cultivate centrifuge bottom and imbed in the inner wall top surface, be equipped with a plurality of atomizing air pocket structures on the humidity board.

Further, cultivate centrifuge includes drain pan, rotating machine, rotary rod, saddle, cultivation pipe, rotating machine fixed mounting is inside the drain pan, the rotary rod bottom imbeds in the rotating machine top, saddle bottom surface and rotary rod top surface fixed connection, cultivate the inside of tub outer loop embedding in the saddle, the cultivation pipe is equipped with a plurality ofly, and the cultivation pipe quantity that every limit of saddle was equipped with is the same.

Furthermore, the culture tube comprises a base plate, a tube wall, a culture chamber and a sealing cover, wherein the top surface of the base plate is fixedly connected with the bottom surface of the tube wall, an outer ring of the culture chamber and an inner ring of the tube wall are integrally formed, the inner ring of the sealing cover is mechanically connected with the top of the culture chamber, and the tube wall is made of a glass material, has good transparency and hardness and cannot react with a biological reagent.

Furthermore, the sealing cover comprises a cover body, a side top plate, a sealing structure and a lower pressing plate, one side of the side top plate is embedded into the cover body, the top of the sealing structure is movably clamped with the inside of the cover body, the other side of the side top plate is overlapped with one side of the sealing structure, the two sides of the lower pressing plate are movably clamped with the bottom of the sealing structure, the cover body is provided with a thread groove structure close to the outer side of the side top plate, and the side top plate is of an irregular water drop-shaped body structure with a smooth.

Further, seal structure is including feeling piece, rolling ring, backup pad, connection handle, reset ring, feeling piece one side and connection handle one side fixed connection, the rolling ring outer loop with be connected handle bottom fixed connection, the inside and connection handle top activity block of reset ring, backup pad one side and connection handle opposite side welded connection, the piece of feeling adopts the silica gel material preparation.

Furthermore, the reset ring comprises an external block, a track, a fixing rod, an inner shaft, a sliding rod and a connecting block, the external block and the track are integrally formed, the inner shaft outer ring is connected with the inner ring of the external block through the fixing rod, the bottom end of the sliding rod is fixedly installed on the inner shaft outer ring, the top end of the sliding rod is overlapped with the inside of the track, the left side of the sliding rod is connected with the right side of the fixing rod through the connecting block, polypropylene arc plates are arranged on two sides of the connecting block, and the inside of the connecting block is supported through.

Advantageous effects

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, through the sealing structure arranged in the sealing cover, in the process of mounting the sealing cover, the lower pressing plate is pushed through the sealing structure by the force of screw thread rotation, so that the lower pressing plate is attached to the liquid level, the liquid in the culture chamber is kept in a full state, the phenomenon that a large amount of bubbles are generated in the centrifugal process to influence the mixing effect is avoided, meanwhile, in the opening process, the lower pressing plate is firstly separated from the liquid level, then the sealing cover is separated, the phenomenon that the internal liquid is partially dragged out to be in contact with the outside due to the pulling effect of the bubbles is avoided, the pollution risk is reduced, and the purity of the sampling is kept.

Drawings

FIG. 1 is a schematic three-dimensional structure of a marine invertebrate cell culture medium according to the present invention.

Fig. 2 is a schematic structural view of a front section of the machine body of the present invention.

FIG. 3 is a schematic structural view of a front cross section of the culture centrifuge of the present invention.

FIG. 4 is a schematic structural view of a front cross section of the culture tube of the present invention.

Fig. 5 is a schematic structural view of a front cross section of the sealing cover of the present invention.

Fig. 6 is a front view of the sealing structure of the present invention.

Fig. 7 is a schematic structural diagram of a front cross-section of a reset ring according to the present invention.

In the figure: a base-1, a machine body-2, a top cover-3, a control panel-4, an inner wall-21, a power supply-22, a temperature plate-23, a liquid bin-24, a humidity plate-25, a culture centrifuge-26, a bottom shell-261, a rotating machine-262, a rotating rod-263, a pallet-264, a culture tube-265, a bottom plate-A1 and a tube wall-A2, culture chamber-A3, sealing cover-A4, cover-A41, side top plate-A42, sealing structure-A43, lower pressing plate-A44, sensing block-B1, rolling ring-B2, supporting plate-B3, connecting handle-B4, resetting ring-B5, external block-B51, track-B52, fixing rod-B53, internal shaft-B54, sliding rod-B55 and connecting block-B56.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are some, not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The first embodiment is as follows:

referring to fig. 1-5, the embodiments of the present invention are as follows: a marine invertebrate cell culture medium structurally comprises a base 1, a machine body 2, a top cover 3 and a control panel 4, wherein the top surface of the base 1 is fixedly connected with the bottom surface of the machine body 2, the bottom surface of the top cover 3 is hinged with the top surface of the machine body 2, and the control panel 4 is embedded in the front surface of the machine body 2; organism 2 includes inner wall 21, power 22, temperature board 23, liquid storehouse 24, humidity board 25, cultivates centrifuge 26, power 22 imbeds in the 21 right sides of inner wall, 23 bottom surface fixed mounting of temperature board in the 21 top surfaces of inner wall, liquid storehouse 24 and the 21 left sides of inner wall are integrated into one piece, humidity board 25 bottom surface fixed mounting in the 21 top surfaces of inner wall, cultivate centrifuge 26 bottom and imbed in the 21 top surfaces of inner wall, be equipped with a plurality of atomizing air pocket structures on the humidity board 25, be favorable to carrying aqueous vapor dispersion ground, keep cultivateing the inside moist in storehouse.

The culture centrifuge 26 includes a bottom case 261, a rotator 262, a rotating rod 263, a tray 264, and a culture tube 265, the rotator 262 is fixedly installed inside the bottom case 261, the bottom of the rotating rod 263 is embedded into the top of the rotator 262, the bottom of the tray 264 is fixedly connected with the top surface of the rotating rod 263, the outer ring of the culture tube 265 is embedded inside the tray 264, the culture tube 265 is provided with a plurality of culture tubes, and the number of the culture tubes 265 on each side of the tray 264 is the same, which is beneficial to maintaining balance and makes the centrifuge more stable without generating vibration.

Wherein, cultivate pipe 265 includes chassis A1, pipe wall A2, cultivates room A3, sealed lid A4, chassis A1 top surface and pipe wall A2 bottom surface fixed connection, cultivate room A3 outer loop and pipe wall A2 inner loop for integrated into one piece, sealed lid A4 inner loop and cultivation room A3 top mechanical connection, pipe wall A2 adopts glass material to make, and transparency and hardness are good, and can not react with biological reagent, are favorable to observing the liquid level state, avoid the material to have the interference to the process of cultivating simultaneously.

Wherein, sealed lid A4 includes lid A41, side roof A42, seal structure A43, holding down plate A44, embedding in lid A41 is inside on one side of side roof A42, seal structure A43 top and the inside activity block of lid A41, side roof A42 opposite side and seal structure A43 one side are folded together, holding down plate A44 both sides and seal structure A43 bottom activity block, lid A41 is close to the side roof A42 outside and is equipped with the thread groove structure, side roof A42 is the glossy irregular water droplet body structure in surface, is favorable to reducing the frictional force during installation to ejecting more smoothly, and the reaction is more sensitive.

Based on the above embodiment, the specific working principle is as follows: putting cell culture solution and cells into a culture centrifuge 26 in a machine body 2, opening a sealing cover A4 of a culture tube 265, putting raw materials, controlling internal temperature and humidity through a control panel 4, completely culturing the cells after a period of time, adding freezing solution into a culture chamber A3, closing a sealing cover A4, embedding the top of a tube wall A2 into a thread groove in the process of screwing the sealing cover A4, pushing a side top plate A42 to enable the side top plate A42 to press a sealing structure A43, moving the sealing structure A43 to the middle, enabling a lower pressing plate A44 to bow downwards in a certain radian, observing the interior of the tube wall A2 at any time until the liquid level is completely overlapped with the lower pressing plate A44, discharging bubbles in the extrusion process, filling the interior of the culture chamber A3 to be in a complete state, and starting a rotary machine 262 to perform centrifugal sampling.

Example two:

referring to fig. 4-5 and 6-7, the embodiment of the present invention is as follows: cultivate pipe 265 including chassis A1, pipe wall A2, cultivates room A3, sealed lid A4, chassis A1 top surface and pipe wall A2 bottom surface fixed connection, cultivate room A3 outer loop and pipe wall A2 inner ring and be integrated into one piece, sealed lid A4 inner ring and cultivation room A3 top mechanical connection, pipe wall A2 adopts glass material to make, and the transparency is good with the hardness, and can not react with biological reagent production, is favorable to observing the liquid level state, avoids the material to have the interference to the process of cultivateing simultaneously.

Wherein, seal structure A43 is including feeling piece B1, rolling ring B2, backup pad B3, connection handle B4, reset ring B5, it is connected with connection handle B4 one side fixed connection to feel piece B1 one side, rolling ring B2 outer loop and connection handle B4 bottom fixed connection, reset ring B5 inside and connection handle B4 top activity block, backup pad B3 one side and connection handle B4 opposite side welded connection, it adopts the silica gel material preparation to feel piece B1, is favorable to strengthening sealed effect, and can not have the kill and kill to the cell of cultivation.

The reset ring B5 comprises an external block B51, a track B52, a fixed rod B53, an internal shaft B54, a sliding rod B55 and a connecting block B56, wherein the external block B51 and the track B52 are integrally formed, an outer ring of the internal shaft B54 is connected with an inner ring of the external block B51 through the fixed rod B53, the bottom end of the sliding rod B55 is fixedly installed on an outer ring of the internal shaft B54, the top end of the sliding rod B55 is overlapped with the inside of the track B52, the left side of the sliding rod B55 is connected with the right side of the fixed rod B53 through the connecting block B56, polypropylene arc plates are arranged on two sides of the connecting block B56, and the inside of the polypropylene arc plates is supported through springs, so that high connection support performance is provided.

Based on the above embodiment, the specific working principle is as follows: when the sealing cover A4 is closed, the side top plate A42 is pushed, the sensing block B1 is pressed inwards, the sensing block B1 is pressed inwards, the connecting handle B4 connected with the sensing block B1 is contracted inwards, the supporting plate B3 is pressed to generate radian at the moment, the lower pressing plate A44 arranged on the rolling ring B2 is pressed to generate downward radian, the sliding rod B55 of the resetting ring B5 is relatively displaced, the connecting block B56 is compressed to generate resetting elastic force, when the sealing cover A4 is opened after the centrifugation is finished, the sealing cover A4 is rotated upwards, the pipe wall A2 is gradually out of matching with the side top plate A42, the elastic force of the connecting block B56 and the elastic force of the supporting plate B3 act simultaneously, the connecting handle B4 is quickly restored, the lower pressing plate B44 is firstly reduced in radian, air is sucked in a gap between the sealing cover A4 to fill the gap and the gap between the air and the liquid surface, the air is separated at the time, and the lower pressing plate A44 is completely separated from the, no adhesion phenomenon due to generation of air bubbles and no contamination of the sample due to adhesion phenomenon when the sealing lid a4 is taken out.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a marine invertebrate cell culture medium, its structure includes base (1), organism (2), top cap (3), control panel (4), its characterized in that:

the top surface of the base (1) is fixedly connected with the bottom surface of the machine body (2), the bottom surface of the top cover (3) is hinged with the top surface of the machine body (2), and the control panel (4) is embedded into the front surface of the machine body (2);

organism (2) include inner wall (21), power (22), temperature board (23), liquid storehouse (24), humidity board (25), cultivate centrifuge (26), power (22) are embedded in inner wall (21) right side, temperature board (23) bottom surface fixed mounting in inner wall (21) top surface, liquid storehouse (24) and inner wall (21) left side be the integrated into one piece, humidity board (25) bottom surface fixed mounting in inner wall (21) top surface, cultivate centrifuge (26) bottom and imbed in inner wall (21) top surface.

2. A marine invertebrate cell culture medium according to claim 1, wherein: the culture centrifuge (26) comprises a bottom shell (261), a rotating machine (262), a rotating rod (263), a tray table (264) and a culture tube (265), wherein the rotating machine (262) is fixedly installed inside the bottom shell (261), the bottom of the rotating rod (263) is embedded into the top of the rotating machine (262), the bottom surface of the tray table (264) is fixedly connected with the top surface of the rotating rod (263), and the outer ring of the culture tube (265) is embedded into the tray table (264).

3. A marine invertebrate cell culture medium according to claim 2, wherein: cultivate pipe (265) including chassis (A1), pipe wall (A2), cultivation room (A3), sealed lid (A4), chassis (A1) top surface and pipe wall (A2) bottom surface fixed connection, cultivation room (A3) outer loop and pipe wall (A2) inner ring be integrated into one piece, sealed lid (A4) inner ring and cultivation room (A3) top mechanical connection.

4. A marine invertebrate cell culture medium according to claim 3, wherein: sealed lid (A4) include lid (A41), side roof (A42), seal structure (A43), holding down plate (A44), embedding in lid (A41) inside side roof (A42) one side, seal structure (A43) top and lid (A41) inside activity block, side roof (A42) opposite side is in the same place with seal structure (A43) one side superpose mutually, holding down plate (A44) both sides and seal structure (A43) bottom activity block.

5. A marine invertebrate cell culture medium according to claim 4, wherein: seal structure (A43) is including feeling piece (B1), rolling ring (B2), backup pad (B3), connection handle (B4), reset ring (B5), it is connected with connection handle (B4) one side fixed connection to feel piece (B1) one side, rolling ring (B2) outer loop and connection handle (B4) bottom fixed connection, reset ring (B5) inside with be connected handle (B4) top activity block, backup pad (B3) one side and connection handle (B4) opposite side welded connection.

6. A marine invertebrate cell culture medium according to claim 5, wherein: the reset ring (B5) comprises an external block (B51), a track (B52), a fixed rod (B53), an internal shaft (B54), a sliding rod (B55) and a connecting block (B56), wherein the external block (B51) and the track (B52) are integrally formed, the external ring of the internal shaft (B54) is connected with the internal ring of the external block (B51) through the fixed rod (B53), the bottom end of the sliding rod (B55) is fixedly installed on the external ring of the internal shaft (B54), the top end of the sliding rod (B55) is overlapped with the inside of the track (B52), and the left side of the sliding rod (B55) is connected with the right side of the fixed rod (B53) through the connecting block (B56).