CN112262846A - Object surface disinfectant for high-grade biosafety laboratory and preparation method thereof - Google Patents
Object surface disinfectant for high-grade biosafety laboratory and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
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- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a material surface disinfectant for a high-grade biosafety laboratory and a preparation method thereof. The physical surface disinfectant not only has a killing effect on highly pathogenic microorganisms such as Ebola virus, SARS coronavirus, novel coronavirus, AIDS virus and the like, but also obviously reduces the corrosion effect of the disinfectant on metal, particularly stainless steel by adding nano magnesium into the disinfectant, thereby avoiding biological safety accidents caused by the leakage of the highly pathogenic microorganisms after metal components of biological safety protection facility equipment in a high-grade biological safety laboratory are corroded, simultaneously reducing the aggregation of the nano magnesium by magnetizing the nano magnesium in the disinfectant to enable the nano magnesium to have magnetism, finally forming a stable dispersion system in the disinfectant, obviously increasing the stability of the disinfectant during storage, and keeping the metal corrosion prevention effect of the disinfectant.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a material surface disinfectant for a high-grade biosafety laboratory and a preparation method thereof.
Background
Various microorganisms exist in nature, some of them can cause diseases of human or animals, and such microorganisms are called pathogenic microorganisms, and are classified into different categories according to their infectivity and pathogenicity, wherein the pathogenic microorganisms capable of causing serious diseases of human or animals are called highly pathogenic microorganisms, such as ebola virus, SARS coronavirus, novel coronavirus, aids virus, and the like. In order to study these highly pathogenic microorganisms, a correspondingly high level of protection is required in biosafety laboratories, i.e. high-grade biosafety laboratories.
In the process of researching the highly pathogenic microorganisms, various measures are taken to ensure that experimenters cannot be infected and the highly pathogenic microorganisms cannot escape from laboratories to the external environment. Therefore, the construction of the high-grade biosafety laboratory is obviously different from other laboratories, and the laboratory construction needs various biosafety protection facilities and equipment to prevent pathogenic microorganisms from escaping and needs a disinfectant to kill highly pathogenic microorganisms. Because the biosafety protection facility equipment comprises an enclosure structure, an airtight door, a chemical shower, a double-door sterilizer, a live-poison wastewater treatment system, a high-efficiency air filter, a laboratory ventilation system and the like, a large amount of metal materials such as stainless steel and the like are used, the metal materials are connected and formed by using a welding process, and the disinfectant is required to be sprayed on the surface of the facility equipment when the disinfectant is used, the disinfectant is ensured to have a killing effect on highly pathogenic microorganisms in a high-grade biosafety laboratory when the disinfectant is selected, and meanwhile, the disinfectant does not have a corrosion effect or has a small corrosion effect on metal components such as stainless steel and the like. However, in the existing disinfectants, the disinfectants with low corrosivity to metals such as stainless steel and the like have poor disinfection effect, cannot be used in high-grade biosafety laboratories due to the fact that highly pathogenic microorganisms cannot be completely killed, and the disinfectants with killing effects on the highly pathogenic microorganisms have different degrees of corrosion effects on the metals such as the stainless steel and the like, and can cause the corrosion of metal components such as the stainless steel and the like after long-term use, so that the biosafety protection effect of the facility equipment is reduced, the service life of the facility equipment is shortened, economic loss is caused, and even the highly pathogenic microorganisms are caused to escape into the external environment of the laboratory, and further the loss of lives and properties of people is caused, and serious social problems are brought. Therefore, it is necessary to develop a disinfectant which can effectively kill highly pathogenic microorganisms and has no corrosivity or low corrosivity on metals such as stainless steel, and the like.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a material surface disinfectant for a high-grade biosafety laboratory and a preparation method thereof. The disinfectant not only solves the high-efficiency killing effect on highly pathogenic microorganisms, but also solves the problems that the existing disinfectant has strong corrosivity on metal, particularly stainless steel materials, reduces the integrity of high-level biosafety laboratory facilities and equipment, and causes biosafety accidents caused by the leakage of the highly pathogenic microorganisms.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the disinfectant consists of quaternary ammonium chloride salt, multienzyme solution, water-soluble silicone oil, emulsifier, preservative, nano magnesium, chelating stabilizer, colorant and the like.
The corrosion effect of the disinfectant on metal, particularly stainless steel materials is reduced by adding the nano magnesium into the disinfectant, but further research shows that the nano magnesium is aggregated and has larger particle size along with the prolonging of the storage time of the disinfectant, so that the nano magnesium cannot be uniformly distributed in the solution, and the corrosion prevention effect is reduced. Therefore, researchers creatively apply a magnetization technology to magnetize the disinfectant, so that nano magnesium in the solution has magnetism to generate mutual repulsion, aggregation of the nano magnesium is further reduced to form a stable dispersion system, the stability of the disinfectant is remarkably improved, the disinfectant can keep the corrosion resistance on metal for a long time, and meanwhile, in the using process of the disinfectant, the nano magnesium is firstly attached to the surfaces of metal such as stainless steel and the like due to the magnetism, so that the corrosion resistance can be better exerted.
The disinfectant produced by the technical scheme has good effect of killing viruses and bacteria, particularly can effectively kill the virulent infectious disease viruses, and simultaneously has obviously reduced corrosivity to metals, when used in high-grade biosafety laboratory, can effectively protect the integrity of laboratory facilities and equipment, ensure the biosafety protection performance of the laboratory facilities and equipment, avoid the highly pathogenic microorganisms from leaking into the external environment of the laboratory to cause biosafety accidents, and simultaneously, the disinfectant is practically nontoxic to human and animals, is convenient to use, has no residue in removal, is environment-friendly and convenient to clean, on the premise of ensuring the efficacy of the product, the cost for realizing the technical requirement is not high, and when the method is used in a high-grade biosafety laboratory, can kill highly pathogenic microorganisms such as Ebola virus, SARS coronavirus, 2019 novel coronavirus, AIDS virus, chikungunya virus, etc. The disinfectant can meet the special requirements of high-grade biosafety laboratories, can also be used for disinfection requirements of public places such as hospitals, stations, hotels and the like, and can kill common pathogenic bacteria in the public places, including but not limited to pseudomonas aeruginosa, candida albicans, staphylococcus aureus, escherichia coli and the like.
The emulsifier in the disinfectant is a compound which can enable a mixed solution of two or more immiscible components to form stable emulsion, and the action principle is that in the emulsification process, a disperse phase is dispersed in a continuous phase in a micro-droplet (micron-sized) form, the emulsifier reduces the interfacial tension of each component in the mixed system, a firmer film is formed on the surface of a micro-droplet or an electric double layer is formed on the surface of the micro-droplet due to the charge given by the emulsifier, the micro-droplets are prevented from aggregating with each other, and the uniform emulsion is kept.
The quaternary ammonium chloride salt in the disinfectant has a function of killing highly pathogenic microorganisms, and is one or a combination of dodecyl dimethyl ammonium chloride, dodecyl dimethyl benzyl ammonium chloride, C12-C14 alkyl benzyl dimethyl ammonium chloride, C12-C16 alkyl benzyl dimethyl ammonium chloride, C12-C14 alkyl [ (phenethyl) methyl ] dimethyl ammonium chloride, dimethyl ethyl benzyl ammonium chloride and C8-C10 dialkyl dimethyl ammonium chloride.
The nano magnesium is creatively added into the disinfectant, so that the corrosion of the disinfectant on metal, particularly stainless steel, can be remarkably reduced, and the preferable mass percentage concentration of the nano magnesium in the disinfectant is 0.015-0.05%, and the particle size is 1-25 nm. And meanwhile, the magnetizer is used for magnetizing the nano magnesium in the solution to form a stable magnetic system, so that the nano magnesium is prevented from being aggregated to keep the corrosion resistance of the nano magnesium.
The multi-enzyme solution in the disinfectant can effectively remove the biological film attached to the metal surface, especially the joints or joint cavities of some facilities and equipment, and the biological film remained in blood and tissue fluid cannot be removed by independent washing, so that the disinfectant has poor effect of killing pathogenic microorganisms due to the existence of the biological film. The multi-enzyme solution can effectively dissolve the biological membrane, ensure that the disinfectant can directly act on instruments, and achieve good disinfection effect. The preferred multi-enzyme solution is a composite solution consisting of liquid amylase, liquid alkaline protease, lipase and cellulase.
The water-soluble silicone oil is added into the disinfectant to effectively lubricate joints or joint cavities of metal instruments, equipment and facilities, and the preferable water-soluble silicone oil is Dow Corning DC-193.
Preservatives are additives which inhibit the action of microorganisms and prevent the spoilage of products, and certain measures must be taken to prevent the infection and proliferation of microorganisms for a certain period of storage. Practice has proved that the antiseptic is one of the most economical, effective and simple to reach the said purpose, and the antiseptic for the disinfecting liquid is preferably methyl paraben, ethyl paraben, benzalkonium chloride, cetylpyridinium bromide and domiphen bromide.
The chelating stabilizer is one or more of glycerol, butanediol, propylene glycol, polyethylene glycol, hexanediol, triethanolamine, tween-80, sodium sulfate, disodium edetate and tetrasodium edetate.
The colorant is one or more of erythrosine, carmine, beet red, amaranth, methylene blue, indigo blue, and lemon yellow.
The invention also discloses a preparation method of the high-grade biological safety laboratory surface disinfectant, which specifically comprises the following steps of but not limited to:
1. dissolving and mixing materials: taking a proper amount of purified water, and uniformly mixing the chelating stabilizer and the water-soluble silicone oil;
2. formation of a stable system: adding the multienzyme solution into the solution for dissolving, adding an emulsifier, homogenizing and emulsifying by using a homogenizer at low temperature, aging for 8 hours for defoaming, forming a stable system formed by uniformly coating multienzyme substances with a chelating stabilizer, and coating the stable system with emulsifying microspheres formed by the emulsifier;
3. final preparation of the disinfectant: and (2) slowly adding the coloring agent and the quaternary ammonium chloride salt into the solution respectively under stirring, uniformly stirring and mixing, then adding a proper amount of nano magnesium, uniformly stirring, quickly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
(III) advantageous effects
The invention provides a high-grade biosafety laboratory surface disinfectant and a preparation method thereof. The method has the following beneficial effects: the disinfectant has remarkable effect in killing highly pathogenic microorganisms such as Ebola virus, SARS coronavirus, 2019 novel coronavirus, AIDS virus, chikungunya virus, etc.; meanwhile, the appropriate amount of nano magnesium is added into the disinfectant, so that the corrosivity of the disinfectant on metal, particularly stainless steel materials, can be obviously reduced, the integrity of high-grade biosafety laboratory facility equipment is protected, the leakage of highly pathogenic microorganisms is avoided, further biosafety accidents are avoided, in addition, a magnetizer is used for magnetizing the nano magnesium in the disinfectant in the preparation process of the disinfectant, and the phenomenon that the nanometer magnesium is gathered in the storage of the disinfectant to lose the effect of preventing metal corrosion is avoided.
The disinfectant can be used for killing highly pathogenic microorganisms in high-grade biosafety laboratories, and can also be used for killing common pathogenic bacteria such as pseudomonas aeruginosa, candida albicans, staphylococcus aureus, escherichia coli and the like in public places such as hospitals, stations, hotels and the like.
Meanwhile, the disinfectant has no toxic or side effect on human bodies and animals, is convenient to use, has no residue after removal, is environment-friendly and convenient to clean, and has low cost for realizing technical requirements on the premise of ensuring the efficacy of products.
Drawings
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a table of statistics of comparative experiments according to the present invention;
FIG. 3 is a schematic diagram showing the effect of the disinfectant with different concentrations of the present invention on killing novel coronavirus.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the embodiment of the present invention provides four technical solutions: the preparation method of the high-grade biological safety laboratory surface disinfectant specifically comprises the following steps:
example 1
S1, dissolving and mixing materials: dissolving 60g of purified water into 8g of sodium sulfate and 2g of sodium carbonate, adding 5g of propylene glycol and 5g of water-soluble silicone oil, and uniformly mixing;
s2, forming a stable system: adding 0.2g of liquid amylase, 0.1g of lipase and 0.05g of liquid alkaline protease into the mixture for dissolving, adding 8g of NP-10, homogenizing and emulsifying at low temperature by using a homogenizer, aging for 8 hours for defoaming, forming a stable system formed by uniformly coating multienzyme substances with 5g of propylene glycol, and coating the stable system by using emulsifying microspheres formed by 8g of NP-10;
s3, final preparation of the disinfectant: slowly adding 0.01g of lemon yellow, 0.5g of Tween-80, 2.5g of dimethyl benzyl ammonium chloride, 2.25g of dimethyl ethyl benzyl ammonium chloride, 0.5g of C12-C16 alkyl benzyl dimethyl ammonium chloride and 0.1g of domiphen bromide into the solution respectively under stirring, uniformly stirring and mixing, adding 0.027g of nano magnesium, supplementing water, weighing, and uniformly stirring. And (3) rapidly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
Example 2
S1, dissolving and mixing materials: dissolving 60g of purified water into 6g of butanediol and 4g of sodium carbonate, adding 4g of glycerol and 6g of water-soluble silicone oil, and uniformly mixing;
s2, forming a stable system: adding 0.2g of cellulase, 0.1g of lipase and 0.05g of liquid alkaline protease into the mixture for dissolving, adding 8g of NP-10, homogenizing and emulsifying at low temperature by using a homogenizer, aging for 8 hours for defoaming, forming a stable system formed by uniformly coating a multienzyme substance with 4g of glycerol, and coating the stable system by using 8g of emulsifying microspheres formed by NP-10;
s3, final preparation of the disinfectant: slowly adding 0.01g of lemon yellow, 0.5g of hexanediol, 2.5g of dimethyl benzyl ammonium chloride, 2.25g of C8-C10 dialkyl dimethyl ammonium chloride, 0.5g of C12-C16 alkyl benzyl dimethyl ammonium chloride and 0.1g of domiphen bromide into the solution respectively under stirring, uniformly stirring and mixing, adding 0.015g of nano magnesium, supplementing water, weighing, and uniformly stirring. And (3) rapidly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
Example 3
S1, dissolving and mixing materials: dissolving 60g of purified water into 8g of polyethylene glycol and 2g of sodium carbonate, adding 5g of butanediol and 5g of water-soluble silicone oil, and uniformly mixing;
s2, forming a stable system: adding 0.2g of liquid amylase, 0.1g of cellulase and 0.05g of liquid alkaline protease into the solution to dissolve the solution, adding 8g of NP-10, homogenizing and emulsifying the solution at a low temperature by using a homogenizer, aging the solution for 8 hours to defoam the solution to form a stable system formed by uniformly coating 5g of butanediol on a multienzyme substance, and coating the stable system by using 8g of emulsified microspheres formed by NP-10;
s3, final preparation of the disinfectant: slowly adding 0.01g of lemon yellow, 0.5g of tetrasodium ethylene diamine tetraacetate, 2.5g of dimethyl benzyl ammonium chloride, 2.25g of dimethyl ethyl benzyl ammonium chloride, 0.5g of C12-C16 alkyl benzyl dimethyl ammonium chloride and 0.1g of domiphen bromide into the solution respectively under stirring, uniformly stirring and mixing, adding 0.035g of nano magnesium, supplementing water, weighing, and uniformly stirring. And (3) rapidly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
Example 4
S1, dissolving and mixing materials: dissolving 60g of purified water into 5g of polyethylene glycol and 5g of sodium carbonate, adding 2g of butanediol and 8g of water-soluble silicone oil, and uniformly mixing;
s2, forming a stable system: adding 0.1g of liquid amylase, 0.2g of cellulase and 0.05g of liquid alkaline protease into the solution to dissolve the solution, adding 8g of NP-10, homogenizing and emulsifying the solution at a low temperature by using a homogenizer, aging the solution for 8 hours to defoam the solution to form a stable system formed by uniformly coating a multienzyme substance with 2g of butanediol, and coating the stable system by using 8g of emulsified microspheres formed by NP-10;
s3, final preparation of the disinfectant: slowly adding 0.1g of lemon yellow, 0.41g of tetrasodium ethylene diamine tetraacetate, 2.4g of dimethyl benzyl ammonium chloride, 2.35g of dimethyl ethyl benzyl ammonium chloride, 0.5g of C12-C16 alkyl benzyl dimethyl ammonium chloride and 0.1g of domiphen bromide into the solution respectively under stirring, uniformly stirring and mixing, adding 0.05g of nano magnesium, supplementing water, weighing, and uniformly stirring. And (3) rapidly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
Comparative experiment
The disinfectant liquid detection center detects the disinfectant liquids prepared in the embodiments 1, 2, 3 and 4 respectively, and the detection contents are as follows: the nano magnesium with different contents in the disinfectant has the effects of corroding metal and killing viruses and bacteria, and meanwhile, data are counted and a statistical table chart is made in the detection process.
Comparative test results
As can be seen from the attached figure 2, four groups of disinfection solutions are respectively prepared by a certain disinfection solution detection center according to the embodiments 1-4 of the invention, and the disinfection solution prepared by the embodiment 1 is obtained according to the final experimental data to have the best effect, wherein the contents of the components are shown in Table 1. The nano magnesium is added into the disinfectant, so that the corrosion of the disinfectant on metal, particularly stainless steel materials, is reduced, and magnesium ions in the disinfectant are magnetized to be magnetic, so that the nano magnesium is favorably attached to the surfaces of the metal such as stainless steel and the like, and a good corrosion prevention effect is achieved. The disinfectant prepared by adding the nano magnesium has good sterilization effect and strong metal corrosion resistance, has good effects of killing viruses and bacteria, particularly can effectively kill the virulent infectious disease viruses, has no toxic or side effect on human bodies, is convenient to use, has no residue after removal, is environment-friendly and convenient to clean, has low cost for realizing the technical requirements on the premise of ensuring the product effects, can meet the requirements of experiments, can meet the requirements of the masses, and ensures the safety of a biological laboratory by utilizing a novel metal corrosion prevention technology.
Table 1 table of contents data of each component in example 1
Firstly, the test result of the object surface disinfectant on the killing of highly pathogenic microorganisms is as follows:
1. the killing effect on the novel coronavirus is as follows: as can be seen from FIG. 3 (note: CPE: cytopathic effect; "+" with CPE; "-" without CPE; 1/2/3 indicates 3 repetitions), the disinfectant prepared in example 1 was diluted to completely kill 2 x 10 ions in 15 seconds at a concentration greater than 1.6%4TCID50A virus; when the concentration of the disinfectant is 0.56%, the disinfectant can completely kill 2 gamma 10 in 30 seconds4TCID50A virus; when the concentration of the disinfectant is 0.19%, 2 gamma 10 can be completely killed in 2 minutes4TCID50A virus.
2. The killing effect on Ebola virus is as follows: when the disinfectant prepared in the embodiment 1 is diluted to 1%, the disinfectant reacts with Ebola virus for 1min to kill 99.999% of virus.
3. When the disinfectant prepared in the embodiment 1 is diluted to 5% at the temperature of 19-21 ℃, the killing logarithm value of the disinfectant on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa is more than 5 after 5min of action, and the killing logarithm value of the disinfectant on candida albicans is more than 4.
4. Under the condition of 19-21 ℃, the disinfection solution prepared in the embodiment 1 is diluted to 5 percent and acts for 5min, the killing logarithm value of the disinfection solution on natural bacteria on the surface of a wooden object is more than 1, and the killing logarithm value of each test on escherichia coli polluted on a cotton cloth piece is more than 3.
II, corrosion resistance experiment data:
the experimental data for the stock solution of example 1 are as follows:
1. the pH value of the product is tested as follows: pH 11.6.
2. The metal corrosion test shows that the corrosion rate of the disinfectant to stainless steel is 0.00011mm/a and basically no corrosion, and the corrosion rate to carbon steel is 0.00244mm/a and basically no corrosion.
3. Particle size distribution.
As can be seen from table 2, the magnetic strengthening of the nano-magnesium is an important means for ensuring the stability of the nano-magnesium in the disinfectant, when the unmagnetized nano-magnesium starts to aggregate after being stored in the disinfectant for 3 months, the particle size of the nano-magnesium starts to gradually increase, and when the magnetically strengthened nano-magnesium is stored in the disinfectant for 12 months, the particle size does not change greatly, the aggregation is very small, so that the magnetic strengthening technology is an important technical means for ensuring the stability of the state of the disinfectant after the nano-magnesium is added.
TABLE 2 disinfectant stability particle size distribution
Thirdly, toxicity test data:
the disinfectant prepared in example 1 was tested, and the results were as follows:
1. the disinfectant stock solution has no irritation to a one-time complete skin irritation strength test of New Zealand rabbits.
2. The stock solution of the disinfectant is practically nontoxic to male and female mice in an acute oral toxicity test.
3. The stock solution of the disinfectant has no micronucleus effect on mouse bone marrow cell chromosomes.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A kind of disinfectant liquid for high-grade biological safety laboratory, its characteristic is: comprises a combination of quaternary ammonium chloride salt, multienzyme solution, water-soluble silicone oil, emulsifier, preservative, nano-magnesium, chelating stabilizer and colorant.
2. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the quaternary ammonium chloride salt is one or more of dodecyl dimethyl ammonium chloride, dodecyl dimethyl benzyl ammonium chloride, C12-C14 alkyl benzyl dimethyl ammonium chloride, C12-C16 alkyl benzyl dimethyl ammonium chloride, C12-C14 alkyl [ (phenethyl) methyl ] dimethyl ammonium chloride, dimethyl ethyl benzyl ammonium chloride and C8-C10 dialkyl dimethyl ammonium chloride.
3. The high-grade biosafety laboratory watch disinfectant according to claim 2, wherein: the mass percentage concentration of the quaternary ammonium chloride salt in the disinfectant is 0.1-10%, and the average molecular weight is between 300 and 450.
4. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the multi-enzyme solution is a composite solution consisting of liquid amylase, liquid alkaline protease, lipase and cellulase.
5. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the emulsifier is one or more of nonylphenol polyoxyethylene ether, octylphenol polyoxyethylene ether, single diglyceride, lecithin, fatty acid ester and polyphosphate.
6. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the preservative is one or more of methylparaben, ethylparaben, benzalkonium chloride, cetylpyridinium chloride, cetylammonium bromide and domiphen bromide, the chelating stabilizer is one or more of glycerol, butanediol, propylene glycol, polyethylene glycol, hexanediol, triethanolamine, tween-80, sodium sulfate, sodium carbonate, disodium ethylenediamine tetraacetic acid and tetrasodium ethylenediamine tetraacetic acid, and the colorant is one or more of erythrosine, carmine, beet red, amaranth, methylene blue, indigo blue and lemon yellow.
7. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the mass percentage concentration of the nano magnesium in the disinfectant is 0.015-0.1%, and the particle size is 1-25 nm.
8. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the mass percentage concentration of the nano magnesium in the disinfectant is 0.015-0.05%.
9. The high-grade biosafety laboratory watch disinfectant according to claim 1, wherein: the water-soluble silicone oil is Dow Corning DC-193.
10. The high-grade biosafety laboratory watch disinfectant according to any one of claims 1 to 9, which is prepared by a method comprising the steps of:
s1, dissolving and mixing materials: uniformly mixing the chelating stabilizer and the water-soluble silicone oil with purified water;
s2, forming a stable system: adding the multienzyme solution into the solution for dissolving, adding an emulsifier, homogenizing and emulsifying by using a homogenizer at low temperature, aging for 8 hours for defoaming, forming a stable system formed by uniformly coating multienzyme substances with a chelating stabilizer, and coating the stable system with emulsifying microspheres formed by the emulsifier;
s3, final preparation of the disinfectant: and (2) slowly adding the coloring agent and the quaternary ammonium chloride salt into the solution respectively under stirring, uniformly stirring and mixing, then adding the nano magnesium, uniformly stirring, quickly magnetizing the nano magnesium in the solution by using a magnetizer to form a stable magnetic system, and finally standing, defoaming, filling and packaging to obtain the final disinfectant.
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| CN113151596A (en) * | 2021-04-02 | 2021-07-23 | 中国医学科学院医学生物学研究所 | Four-level biological safety laboratory virus chemical shower disinfection verification method |
| CN113151596B (en) * | 2021-04-02 | 2023-08-08 | 中国医学科学院医学生物学研究所 | Chemical shower disinfection verification method for viruses in four-level biosafety laboratory |
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| CN112262846B (en) | 2021-11-09 |
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