CN112262799A - Nutrition regulation and control method for improving reproductive performance and larva quality of litopenaeus vannamei - Google Patents
Nutrition regulation and control method for improving reproductive performance and larva quality of litopenaeus vannamei Download PDFInfo
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- CN112262799A CN112262799A CN202011215186.4A CN202011215186A CN112262799A CN 112262799 A CN112262799 A CN 112262799A CN 202011215186 A CN202011215186 A CN 202011215186A CN 112262799 A CN112262799 A CN 112262799A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a nutrition regulation and control method for improving the reproductive performance and the larva quality of parent litopenaeus vannamei, which belongs to the field of aquatic animal nutrition, and is applied to a nutrition strengthening stage of parent litopenaeus vannamei, wherein the nutrition strengthening stage is divided into two stages, wherein the first stage of nutrition strengthening is that nutrition strengthening feed is fed for 70 days from 120 days before spawning to 50 days before spawning, and the second stage of nutrition strengthening is that nutrition strengthening feed and clamworm are mixed and fed for 20 days from 50 days before spawning to 30 days before spawning; on the basis that basic nutritional requirements meet the development of parent litopenaeus vannamei, the content of astaxanthin and vitamin A in the nutrition-enriched feed is controlled, so that the content of astaxanthin and vitamin A in the nutrition-enriched feed is 100mg/kg feed and 20000IU/kg feed respectively. Compared with a control group, the method provided by the invention has the advantage that the reproductive performance and the larva quality of the parent litopenaeus vannamei are obviously improved.
Description
Technical Field
The invention belongs to the field of aquatic animal nutrition, and particularly relates to a nutrition regulation and control method for improving the reproductive performance and the larva quality of litopenaeus vannamei parent shrimps in a parent shrimp nutrition strengthening stage.
Background
The litopenaeus vannamei (Litopenaeus vannamei), also known as Penaeus vannamei, is the prawn culture variety with the largest mariculture yield in China and one of the most main crustacean economic varieties in the world, and accounts for more than 50% of the total yield of the whole crustacean. In the actual production, the breeding and cultivating process of the parent litopenaeus vannamei comprises two stages: the first stage is a nutrition strengthening stage, which generally lasts for more than 2 months and effectively promotes the development of the gonads of the parent shrimps through artificial nutrition strengthening; the second stage is a breeding and spawning stage, and the characteristic of the second stage is that the unilateral eyestalk of female parent shrimps is removed to promote the spawning of the parent shrimps. At present, biological baits such as clamworms, oysters, squids and the like are fed from a nutrition strengthening stage to a breeding and spawning stage in the breeding and breeding process of parent shrimps, but the biological baits are expensive and have the defects of single nutrient component, unstable quality, carrying of prawn pathogeny and the like, so that the breeding and breeding of parent shrimps of litopenaeus vannamei are severely restricted, and the quality of the seedlings of the litopenaeus vannamei is further restricted. Therefore, the development of the litopenaeus vannamei parent shrimp feed which has comprehensive and balanced nutritive value and does not carry pathogens so as to replace biological bait to the maximum extent is a problem to be solved urgently in the litopenaeus vannamei breeding process in China.
Disclosure of Invention
The invention aims to provide a nutrition regulation and control method for improving the reproductive performance and the larva quality of the litopenaeus vannamei parent shrimps in the nutrition strengthening stage of the parent shrimps. Aiming at the nutrition strengthening stage of the breeding and cultivation of the parent litopenaeus vannamei, the invention focuses on the carotenoid (astaxanthin) and the vitamin A which have important effects on the gonad development, regulates and controls the gonad development of the parent litopenaeus vannamei by controlling the addition amount and the proportion of the astaxanthin and the microorganism A in the parent litopenaeus vannamei feed, enhances the reproductive performance and the fry quality of the parent litopenaeus vannamei, and fills the defects of the prior art in the field.
The invention is realized according to the following technical scheme:
a nutrition regulation and control method for improving the reproductive performance and the larva quality of parent litopenaeus vannamei is applied to the nutrition strengthening stage of the parent litopenaeus vannamei; the nutrition strengthening stage comprises 90 days from 120 days before spawning to 30 days before spawning, and is divided into two stages, wherein the first stage of nutrition strengthening, namely 70 days from 120 days before spawning to 50 days before spawning, is used for feeding nutrition strengthening feed, and the second stage of nutrition strengthening, namely 20 days from 50 days before spawning to 30 days before spawning, is used for mixed feeding of the nutrition strengthening feed and the clamworms.
Furthermore, on the basis that the basic nutritional requirements meet the development of parent litopenaeus vannamei, the content of astaxanthin and vitamin A in the nutrition-enriched feed is controlled, so that the content of astaxanthin and vitamin A in the nutrition-enriched feed is respectively 100mg/kg feed and 20000IU/kg feed.
Further, the purity of astaxanthin was 10% and the purity of vitamin A was 500000 IU.
Compared with the prior art, the invention has the beneficial effects that:
(1) compared with a control group, the nutrition regulation and control method provided by the invention has the advantages that the reproductive performance and the larva quality of the parent litopenaeus vannamei are obviously improved. Comprises the steps of improving the gonad index of female shrimps in the late period of parent shrimp nutrition enrichment (averagely improving by 26%), averagely improving by 32%, hatchability of fertilized eggs (averagely improving by 31%), the number of nauplius (averagely improving by 22%) and the number of flea larvae (averagely improving by 20%).
(2) By applying the nutrition regulation and control method disclosed by the invention, the use of clamworms can be reduced in the breeding process of the parent litopenaeus vannamei, the cost is reduced, and the economic benefit is improved.
Drawings
FIG. 1 shows the effect of astaxanthin and vitamin A in different levels in feed on gonadal index of female shrimp in the late stage of nutrition enrichment of parent Litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 2 shows the effect of different levels of astaxanthin and vitamin A in the feed on the egg laying amount of parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 3 shows the effect of different levels of astaxanthin and vitamin A in the feed on the hatchability of the fertilized eggs of litopenaeus vannamei parent shrimps. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
Figure 4 effect of different levels of astaxanthin and vitamin a in the feed on the number of nauplii. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 5 the effect of different levels of astaxanthin and vitamin A in the feed on the number of flea larvae. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 6 the effect of feeding clamworm and feed on the gonadal index of female shrimp in the late stage of the nutrition enrichment of parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 7 the effect of feeding clamworm and feed on the egg laying amount of parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 8 the effect of feeding clam worms and feed on the hatchability of the fertilized eggs of the parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
Figure 9 effect of feeding clamworm and feed on number of nauplii. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 10 effect of feeding Nereid and feed on flea larval numbers. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 11 shows the effect of feeding different feed formulas on the gonad index of female shrimps in the later period of the nutrition enrichment of parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 12 is the effect of feeding different formula feeds on the egg laying amount of parent litopenaeus vannamei. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 13 the effect of feeding different formula feeds on the hatchability of fertilized eggs of litopenaeus vannamei parent shrimps. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
Figure 14 effect of feeding different formula on number of nauplii. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
FIG. 15 effect of ingestion of different formula on flea larvae numbers. Data are presented as mean ± standard error (n ═ 3), with significant differences between data columns without identical letters (P < 0.05).
Detailed description of the preferred embodiments
The technical solutions of the present invention are further illustrated by the following specific examples, but the scope of the present invention is not limited in any way by the examples:
example 1 Effect of different levels of astaxanthin and vitamin A in the feed on the reproductive Performance and larval quality of Litopenaeus vannamei parent shrimp
(1) Experimental design and feed formula
The experiment uses fish meal, shrimp meal and mussel powder as protein sources, fish oil and soybean oil as fat sources and high gluten meal as glycogen to prepare a control group basic feed. On the basis, 0.05% of astaxanthin was added to the experimental group 1 so that the astaxanthin content of the feed was 50mg/kg of feed dry matter; experiment group 2 added 0.1% astaxanthin to make the astaxanthin content of the feed 100mg/kg feed dry matter; experiment group 3 added 0.1% + 0.002% astaxanthin and vitamin A to make the astaxanthin and vitamin A content of the feed 100mg/kg and 10000IU feed dry matter respectively; experiment group 4 added astaxanthin and vitamin A in amounts of 0.1% + 0.004% respectively, so that the astaxanthin and vitamin A contents of the feed are 100mg/kg and 20000IU feed dry matter respectively. Carboxymethyl cellulose was used as an equivalent substitute for the control group. Wherein the purity of astaxanthin is 10%, and the purity of vitamin A is 500000 IU. The specific feed formulation is shown in table 1.
TABLE 1 feed formulations containing different levels of astaxanthin and vitamin A (%, feed dry matter)
(2) Shrimp and breeding management for experiment
The experiment selects the litopenaeus vannamei with the uniform size of 5 months old and undeveloped gonads, the initial weight of the female shrimps is 35.20g, and the initial weight of the male shrimps is 34.85 g. The experiment is provided with 5 treatment groups, the feeds of a control group and the experimental groups 1-4 are respectively fed, each treatment group is provided with 3 parallel groups, 25 female shrimps and 25 male shrimps are respectively placed in each parallel group, and the female shrimps and the male shrimps are separately cultured. The experimental feed is fed for 70 days in the first stage of nutrition enhancement, namely 120 days before spawning to 50 days before spawning, and the experimental feed and the clamworm are mixed and fed for 20 days in the second stage of nutrition enhancement, namely 50 days before spawning to 30 days before spawning. The feeding frequency of all treatment groups is 2 times of 7:00 am and 17:00 pm every day, and the feeding amount is determined according to the ingestion condition of the parent shrimps, so that all shrimps are guaranteed to achieve apparent satiation feeding. After the nutrition strengthening stage is finished, removing the unilateral eyestalk of the female shrimp by a forceps-scalding method to promote maturity, breeding and spawning, and feeding all treatment groups with clamworms and squids until spawning.
In the experimental process, the seawater is used after being precipitated in a sedimentation tank and subjected to sand filtration and disinfection, the culture water temperature is 25-28 ℃, the salinity is 26-30, the pH is 7.8-8.0, the dissolved oxygen is higher than 6.5mg/L, and the ammonia nitrogen is less than 0.5 mg/L.
(3) Sample collection
And after the nutrition strengthening stage is finished, randomly weighing 3 female shrimps in parallel, weighing the weight of the female shrimps, weighing the gonads, and calculating the gonad index after the nutrition strengthening. After the eye handle of a female parent shrimp is removed, feeding is continuously carried out by using clamworms and squids until the female parent shrimp spawns, the same parallel sexually mature male shrimp is selected for artificial insemination, counting of fertilized eggs is completed within 2 hours after spawning is finished, then the hatching barrel is turned for 1 time every 1 hour until a nauplius larva is hatched, sampling and counting are carried out, then the nauplius larva is continuously waited to be transformed into flea-shaped nauplius in the hatching barrel, and sampling and counting are carried out.
(4) Statistical analysis
The calculation formula of the gonad index and the hatching rate of the fertilized eggs is as follows:
gonadal index of 100 x gonadal weight/parent shrimp weight
Hatchability of fertilized eggs is 100 times average number of nauplii/average number of eggs laid by the same individual parent shrimp
The experimental data are expressed as mean ± sem, and the one-way anova was performed with SPSS16.0, with P <0.05 as a significant difference.
(5) Results of the experiment
Compared with a control group, the feed added with the astaxanthin and the vitamin A improves the reproductive performance and the larva quality of parent litopenaeus vannamei, and reaches the maximum value when the content of the astaxanthin and the vitamin A in the experimental group 4, namely the feed, is 100mg/kg and 20000IU/kg respectively. Compared with the control group, the gonadal index of the litopenaeus vannamei parent shrimps fed with the feed of the experimental group 1 is averagely improved by 26 percent (figure 1), the egg laying amount is averagely improved by 32 percent (figure 2), the hatchability of fertilized eggs is averagely improved by 31 percent (figure 3), the number of nauplii is averagely improved by 22 percent (figure 4), and the number of flea-shaped larvae is averagely improved by 20 percent (figure 5).
Example 2 comparative experiment of feeding Litopenaeus vannamei parent shrimps with the compound feed and fresh live bait prepared by the method of the present invention
(1) Experimental design and feed formula
Fresh and alive baits of clamworms and squids are fed to the experimental control group, the experimental feed is prepared by selecting the formula of the experimental group 4 in the example 1, and the specific formula of the feed is shown in a table 2.
Table 2 feed formulation of experimental group (%, feed dry matter)
(2) Shrimp and breeding management for experiment
The experiment selects the litopenaeus vannamei with the uniform size of 6 months old and undeveloped gonads, the initial weight of the female shrimps is 36.37g, and the initial weight of the male shrimps is 35.12 g. The experiment is provided with 2 treatment groups, each treatment group is provided with 3 parallel treatment groups, 25 female shrimps and 25 male shrimps are placed in parallel, and the female shrimps and the male shrimps are separately cultured. The whole process of feeding fresh and live baits of clamworms and squids is taken as a control group, the experimental group feeds the compound feed in the nutrition strengthening stage, and the breeding and spawning stage feeds the fresh and live baits of clamworms and squids. The time and method for feeding the feed for enhancing the nutrition are the same as those in example 1, and briefly, the feed is fed in the first stage of enhancing the nutrition, and the feed and the clamworm are mixed and fed in the second stage. The feeding frequency of the experimental group and the control group is consistent, the feeding frequency is 2 times per day, the feeding frequency is respectively 2 times at 7:00 am and 17:00 pm, the feeding amount is determined according to the ingestion condition of the parent shrimps, and the ingestion of the shrimps is ensured to reach the apparent satiation level. After the nutrition enrichment stage is finished, as in example 1, the unilateral eyestalk of the female shrimp is removed by a tweezer ironing method to promote ripening, and in a breeding and spawning stage, all treatment groups are fed with clamworms and squids until spawning.
In the experimental process, the seawater is used after being precipitated in a sedimentation tank and subjected to sand filtration and disinfection, the culture water temperature is 25-28 ℃, the salinity is 26-30, the pH is 7.8-8.0, the dissolved oxygen is higher than 6.5mg/L, and the ammonia nitrogen is less than 0.5 mg/L.
(3) Sample collection
The sample was collected as in example 1. And 3 female shrimps are randomly taken in parallel, the weight of each female shrimp is weighed, then the female shrimp is dissected to take a gonad scale, and the gonad index after nutrition enrichment is calculated according to a formula. Then, the eye handle of the female parent shrimp is removed, feeding is continued to be carried out by using the clamworm and the squid until the female parent shrimp spawns, the male shrimp with the same parallel sex maturity is selected for artificial insemination, and the fertilized egg counting is completed within 2 hours after the spawning is finished. Then, turning over the hatching barrel for 1 time every 1h until the hatched nauplii are sampled and counted, and then continuing waiting for the nauplii to be transformed into flea-shaped nauplii in the hatching barrel, sampling and counting.
(4) Statistical analysis
The calculation formula of the gonad index and the hatching rate of the fertilized eggs is as follows:
gonadal index of 100 x gonadal weight/parent shrimp weight
Hatchability of fertilized eggs is 100 times average number of nauplii/average number of eggs laid by the same individual parent shrimp
Experimental data are expressed as mean ± standard error with a T test using SPSS16.0 to achieve significant differences with P < 0.05.
(5) Results of the experiment
Compared with feeding of fresh and live baits, although parent shrimps eating the feed have a tendency of reducing gonad index, egg laying amount, hatching rate of fertilized eggs, number of nauplii and number of fleas (figures 6-10), the statistical difference (P >0.05) does not exist, which indicates that the compound feed prepared by the method can replace the fresh and live baits with the feed at the nutrition strengthening stage, thereby realizing the purpose of reducing the use of the fresh and live baits to the maximum extent in the breeding and breeding of the parent shrimps and reducing the risk of exogenous pathogens brought by the fresh and live baits.
Example 3 application of the Litopenaeus vannamei parent shrimp feed nutrition regulation method of the present invention in different feed formulas
(1) Experimental design and feed formula
In order to study the application effect of the nutrition regulation method in different feed formulas for litopenaeus vannamei parent shrimps, different feed formulas are designed under the condition that the contents of basic feed protein (about 48%) and fat (15%) are similar to those in example 1. Wherein, the control group feed does not contain astaxanthin and vitamin A, the experimental group feed contains 100mg/kg astaxanthin and 20000IU vitamin A respectively, the purity of all astaxanthin in the experiment is 10%, and the purity of vitamin A is 500000 IU. The specific feed formulation is shown in table 3.
Table 3 feed formulation and nutrient composition (%, feed dry matter) of experiment
(2) Shrimp and breeding management for experiment
The same litopenaeus vannamei boone with undeveloped gonads as in example 2 was selected for this experiment, with an initial weight of 36.37g for female shrimps and 35.12g for male shrimps. The experiment is provided with 2 treatment groups, each treatment group is provided with 3 parallel treatment groups, 25 female shrimps and 25 male shrimps are placed in parallel, and the female shrimps and the male shrimps are separately cultured. In the nutrition enrichment stage, the control group and the experimental group are fed with corresponding compound feeds, and the time and the method for feeding the feeds with specific nutrition enrichment are the same as those in example 1, namely, the feeds are fed in the first stage of nutrition enrichment and the feeds and the clamworms are mixed and fed in the second stage. The feeding frequency of the experimental group and the control group is consistent, the feeding frequency is 2 times per day, the feeding frequency is respectively 2 times at 7:00 am and 17:00 pm, the feeding amount is determined according to the ingestion condition of the parent shrimps, and the ingestion of the shrimps is ensured to reach the apparent satiation level. After the nutrition enrichment stage is finished, as in example 1, the unilateral eyestalk of the female shrimp is removed by a tweezer ironing method to promote ripening, and in a breeding and spawning stage, all treatment groups are fed with clamworms and squids until spawning.
In the experimental process, the seawater is used after being precipitated in a sedimentation tank and subjected to sand filtration and disinfection, the culture water temperature is 25-28 ℃, the salinity is 26-30, the pH is 7.8-8.0, the dissolved oxygen is higher than 6.5mg/L, and the ammonia nitrogen is less than 0.5 mg/L.
(3) Sample collection
The sample was collected as in example 1. And 3 female shrimps are randomly taken in parallel, the weight of each female shrimp is weighed, then the female shrimp is dissected to take a gonad scale, and the gonad index after nutrition enrichment is calculated according to a formula. Then, the eye handle of the female parent shrimp is removed, feeding is continued to be carried out by using the clamworm and the squid until the female parent shrimp spawns, the male shrimp with the same parallel sex maturity is selected for artificial insemination, and the fertilized egg counting is completed within 2 hours after the spawning is finished. Then, turning over the hatching barrel for 1 time every 1h until the hatched nauplii are sampled and counted, and then continuing waiting for the nauplii to be transformed into flea-shaped nauplii in the hatching barrel, sampling and counting.
(4) Statistical analysis
The calculation formula of the gonad index and the hatching rate of the fertilized eggs is as follows:
gonadal index of 100 x gonadal weight/parent shrimp weight
Hatchability of fertilized eggs is 100 times average number of nauplii/average number of eggs laid by the same individual parent shrimp
Experimental data are expressed as mean ± standard error with a T test using SPSS16.0 to achieve significant differences with P < 0.05.
(5) Results of the experiment
Compared with the control group feed, the experimental group feed provided by the invention has the advantages that the gonad index (increased by 23 percent, shown in figure 11), the egg laying amount (increased by 27 percent, shown in figure 12), the fertilized egg hatchability (23 percent, shown in figure 13), the number of nauplii (increased by 16 percent, shown in figure 14) and the number of flea-shaped larvae (increased by 14 percent, shown in figure 15) of the parent litopenaeus vannamei are obviously increased by applying the experimental group feed disclosed by the invention (P is less than 0.05). The method is not limited by the basic feed formula, and has the effect of improving the reproductive performance and the larva quality of the litopenaeus vannamei parent shrimps when being applied to different feed formulas.
Claims (2)
1. A nutrition regulation and control method for improving the reproductive performance and the larva quality of parent litopenaeus vannamei is characterized in that the method is applied to the nutrition strengthening stage of the parent litopenaeus vannamei; the nutrition strengthening stage comprises 90 days from 120 days before spawning to 30 days before spawning, and is divided into two stages, wherein the first stage of nutrition strengthening, namely 70 days from 120 days before spawning to 50 days before spawning, is used for feeding nutrition strengthening feed, and the second stage of nutrition strengthening, namely 20 days from 50 days before spawning to 30 days before spawning, is used for mixed feeding of the nutrition strengthening feed and the clamworms; on the basis that basic nutritional requirements meet the development of parent litopenaeus vannamei, the content of astaxanthin and vitamin A in the nutrition-enriched feed is controlled, so that the content of astaxanthin and vitamin A in the nutrition-enriched feed is 100mg/kg feed and 20000IU/kg feed respectively.
2. The method according to claim 1, characterized in that the astaxanthin is 10% pure and the vitamin A is 500000IU pure.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106306554A (en) * | 2016-08-16 | 2017-01-11 | 中国水产科学研究院黄海水产研究所 | Litopenaeus vannamei parent prawn feed capable of replacing fresh and living bait |
| CN109122445A (en) * | 2018-08-27 | 2019-01-04 | 中国水产科学研究院黄海水产研究所 | A kind of parent shrimp rearing method improving litopenaeus vannamei breeding performance |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106306554A (en) * | 2016-08-16 | 2017-01-11 | 中国水产科学研究院黄海水产研究所 | Litopenaeus vannamei parent prawn feed capable of replacing fresh and living bait |
| CN109122445A (en) * | 2018-08-27 | 2019-01-04 | 中国水产科学研究院黄海水产研究所 | A kind of parent shrimp rearing method improving litopenaeus vannamei breeding performance |
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| Title |
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| 张玉玲等: "饲料中添加南极磷虾粉对营养强化阶段凡纳滨对虾亲虾产卵的影响", 《中国海洋大学学报(自然科学版)》 * |
| 杜少波等: "亲虾营养需求研究进展", 《热带海洋学报》 * |
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