CN112245497A - Chinese medicinal preparation for sterilizing, diminishing inflammation and promoting wound healing and preparation method thereof - Google Patents
Chinese medicinal preparation for sterilizing, diminishing inflammation and promoting wound healing and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing. The Chinese medicinal preparation comprises Borax, pulvis Talci, dried Alumen, Chinese gall, Realgar, Myrrha, ovum gallus Domesticus album, radix Angelicae Dahuricae, cortex Phellodendri, Borneolum Syntheticum, Lithargyrum, Notoginseng radix, Saviae Miltiorrhizae radix, radix et caulis Opuntiae Dillenii and herba Rosmarini officinalis; the traditional Chinese medicine preparation prepared by the invention can promote wound healing, accelerate wound convergence, reduce exudation of tissue fluid, keep the wound surface dry and improve the wound healing quality; reducing local wound edema degree, forming a protective film on the wound surface to prevent wound infection, and has the effects of sterilizing and diminishing inflammation, relieving swelling and pain, promoting blood circulation by removing blood stasis, removing necrotic tissue and promoting granulation, and promoting wound healing.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing.
Background
The wound is infected due to bacterial breeding caused by slight improper nursing, and the common symptoms are red swelling, pain and secretion at the wound position or accompanied by fever and increase of white blood cells, and the wound is difficult to heal, so severe patients need to perform debridement operation. In response to such circumstances, the conventional treatment method is to use broad-spectrum antibiotics for antibacterial therapy, but in recent years, due to the large amount of antibiotic drugs, various bacteria or fungi are resistant, so that the effect is not ideal.
In recent years, in order to avoid abuse of antibiotic drugs, traditional Chinese medicines are gradually used by the public due to small side effects and remarkable effects, but most of the existing debrided traditional Chinese medicine preparations on the market have two conditions, so that side effects to a certain extent often exist in quick effect, the effect of the medicines with small side effects is slow, and the effective or curative effect can be achieved in a long time, so that the traditional Chinese medicine preparation with remarkable effect and small side effects is urgently needed to be provided.
Disclosure of Invention
Aiming at the defects and problems of sterilization and inflammation diminishing of the conventional traditional Chinese medicine preparation at present, the invention provides the traditional Chinese medicine preparation which has obvious effect and small side effect and is capable of sterilizing and diminishing inflammation and promoting wound healing.
The technical scheme adopted by the invention for solving the technical problems is as follows: a traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises the following raw materials in parts by weight: 1-5 parts of borax, 1-5 parts of talcum, 1-2 parts of dried alum, 1-5 parts of entomogenous drug, 0.1-1 part of realgar, 1-3 parts of myrrh, 5-10 parts of egg white, 2-4 parts of angelica dahurica, 1-3 parts of phellodendron, 0.1-0.5 part of borneol, 0.2-0.5 part of lithargite, 3-5 parts of pseudo-ginseng, 2-5 parts of salvia miltiorrhiza, 5-15 parts of cactus and 5-15 parts of rosemary.
The traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises the following raw materials in parts by weight: 3 parts of borax, 3 parts of talcum, 1.5 parts of dried alum, 3 parts of Chinese ivy herb, 0.5 part of realgar, 2 parts of myrrh, 8 parts of egg white, 3 parts of angelica dahurica, 2 parts of phellodendron, 0.2 part of borneol, 0.5 part of lithargite, 4 parts of pseudo-ginseng, 3 parts of salvia miltiorrhiza, 10 parts of cactus and 10 parts of rosemary.
The traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises the following raw materials in parts by weight: the feed comprises the following raw materials in parts by weight: the feed comprises the following raw materials in parts by weight: 4 parts of borax, 4 parts of talcum, 1.5 parts of dried alum, 3 parts of Chinese ivy herb, 0.5 part of realgar, 2 parts of myrrh, 10 parts of egg white, 3 parts of angelica dahurica, 2 parts of phellodendron, 0.2 part of borneol, 0.3 part of litharge, 4 parts of pseudo-ginseng, 4 parts of salvia miltiorrhiza, 15 parts of cactus and 15 parts of rosemary.
The invention also provides a preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing, which comprises the following steps: the method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral medicinal powder, sieving the mineral medicinal powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder for 3-4 hours at the temperature of 180 ℃, and cooling the powder to room temperature for later use after sterilization;
step two, rapidly freezing the egg white at-18 ℃ for 3-5h, freezing, freeze-drying for 12-24h, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed and broken, putting the rosemary into an aqueous solution, heating and distilling the rosemary for 0.5 to 1 hour to extract rosemary perfume extract, adding n-hexane into the rosemary perfume extract to extract for 2 to 3 times, combining the extract, filtering the combined extract, and removing the solvent to obtain rosemary essential oil;
respectively crushing and mixing radix angelicae, golden cypress, pseudo-ginseng, radix salviae miltiorrhizae, lacca mollissima and cactus, adding 5-10 times of 95% ethanol into the mixture, extracting for 48-72 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha haplocalyx essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
In the preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing, the wall breaking treatment method in the third step is enzymolysis.
In the preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing, the enzyme used for enzymolysis comprises one or more of amylase, cellulase, pectinase and papain.
The preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises the fourth step of adding 8 times of 95% ethanol into the mixture to extract for 60 hours.
The method comprises the following steps: in the formula, borax and boron-containing mineral substances have the effects of bacteriostasis, antisepsis and skin mucosa protection.
The dried alum, also called calcined alum, enters lung, spleen, stomach and large intestine channels and has the functions of dissolving phlegm, drying dampness, checking diarrhea, stopping bleeding and detoxifying.
Talcum, a common silicate mineral, has the effects of inducing diuresis for treating stranguria, clearing summer-heat, relieving fever, eliminating dampness and healing sore.
Borneol, pungent and cool in flavor, is indicated for blood-shot eyes with swelling pain, sore throat, aphtha, sores and abscess with swelling pain, and unhealing ulcer.
Realgar, entering liver and large intestine meridian, has effects of removing toxic substance, killing parasite, eliminating dampness and eliminating phlegm, and can be used for treating carbuncle, furuncle, snake and insect bite.
Cortex Phellodendri, entering kidney and bladder meridians, has effects of clearing heat, eliminating dampness, removing toxic substance and treating sore.
Radix Angelicae Dahuricae is warm in nature and pungent in flavor, has effects of dispelling pathogenic wind and removing dampness, promoting blood circulation and expelling pus, promoting granulation and relieving pain, and can be used for treating superficial infection, pyocutaneous disease and skin pruritus.
Lithargyrum enters liver and spleen meridians, and has effects of eliminating dampness, killing parasite, removing toxic substance, astringing, and preventing corrosion.
The pseudo-ginseng is cool in nature and sweet in taste, has the functions of clearing heat and removing toxicity, stretching blood vessels, resisting inflammation and resisting oxidation, and has the effects of diminishing inflammation and easing pain, removing toxicity and cooling blood, and activating blood and dredging collaterals.
Myrrha, with bitter taste and mild nature, has the effects of removing blood stasis, relieving pain, detumescence and promoting granulation, and can be used for treating traumatic injury, blood stasis, carbuncle, swelling and pain, etc.
Salvia miltiorrhiza, bitter in taste and slightly cold in nature, enters heart and liver meridians, and has the effects of activating blood circulation to dissipate blood stasis, stimulating menstrual flow to relieve pain, cooling blood and eliminating carbuncle.
The cactus has the functions of clearing away heat and toxic material, eliminating blood stasis, eliminating swelling and stopping bleeding, contains several kinds of trace elements and has excellent functions of protecting wound and promoting wound healing.
Rosemary contains antioxidant components such as rosmarinic acid, carnosic acid, ursolic acid and rosmarinic acid, has strong astringent effect, and can promote blood circulation and stimulate skin regeneration.
The egg white contains abundant proteins, and has effects of removing toxic substances and promoting wound healing.
The medicine for decayed teeth is cool in nature and bitter in taste, and has the functions of resisting virus, resisting bacteria and regulating immunity.
The effectiveness of the Chinese medicinal preparation of the present invention is further illustrated by the following test examples.
First, skin irritation test
The Chinese medicinal powder prepared in the embodiment 1 of the invention is taken as a test medicament, 70% ethanol is taken as a reference medicament, and distilled water is taken as a blank reference.
Healthy white rabbits were selected as test animals. The rabbits 6 were randomly divided into a complete skin group and a damaged skin group according to sex and weight, and each group had 3 rabbits.
Complete skin group: the rabbits were depilated bilaterally symmetrically in the dorsal spine 24h before administration, ranging approximately 50cm on each side. The upper half of the left side is distilled water (blank control zone), the lower half is 70% alcohol control zone (drug control zone), and the right side is the powder of the example (test zone). After 24h of depilation, the left upper half area of the intact skin group was coated with 0.5mL of distilled water, the lower half area with 0.5mL of 70% ethanol, and the right side was covered with the powder of the example, and the test area was covered with two layers of gauze and applied 1 time a day.
Group of damaged skin: the skin in the depilatory area was abraded with a needle before administration to achieve abrasion of the epidermis, no damage to the dermis, and mild bleeding, and the administration was performed in the same manner as in the intact skin group. After the administration was fixed for 6 hours, the cover was removed and the administration site was observed for erythema and edema. Applied 1 time daily for 5 days. The skin irritation of the rabbits of different groups after application was observed.
As a result: when the medicinal powder is continuously administered to the skin for 5 days, no erythema, allergy or edema occurs on the intact skin or the damaged skin. The wounds of the damaged skin group are completely healed after the medicine is stopped, the crust peels off, and the phenomena of pigmentation and rough skin are not seen at the wounds, which shows that the traditional Chinese medicine powder prepared by the invention has no stimulation to healthy skin and damaged skin, belongs to a non-irritant medicine and is safe to apply.
Second, in vitro bacteriostatic drug effect test
The in vitro bacteriostasis test of the medicine refers to the test of testing the capacity of the medicine to inhibit or kill bacteria in vitro. In the experiment, the traditional Chinese medicine powder prepared in example 1 is used as a test medicine, tetracycline is used as a control medicine group, and the bacteriostasis conditions of the traditional Chinese medicine powder and the tetracycline to staphylococcus aureus, pseudomonas aeruginosa and salmonella are calculated respectively. Respectively selecting single colonies of staphylococcus aureus, pseudomonas aeruginosa and salmonella, dissolving in 10mL of sterile water, oscillating uniformly, and adjusting turbidity to 1 × 106 CFU/mL。
1g of the traditional Chinese medicine powder prepared in the example 1 is respectively dissolved in 10mL of sterile water and is subjected to gradient dilution to obtain 0.01g/mL and 0.001g/mL liquid medicines which are used as a medicine test group, a 0.01g/mL nychoulian solution is used as a medicine control group, and the sterile water is used as a blank control group.
The paper sheets are soaked for 2 min, the soaked paper sheets are placed in 20 culture dishes and dried in a drying box at the temperature of 60 ℃, and the paper sheets of the blank control group are not soaked. Under the aseptic operation, firstly adding 20 mu L of suspension of staphylococcus aureus, pseudomonas aeruginosa and salmonella and 20 mL of culture medium into a culture dish, then dividing the dried paper sheets into 4 parts (except for a normal control group without sticking the paper sheets), respectively placing the paper sheets into the culture dishes of the staphylococcus aureus, the pseudomonas aeruginosa and the salmonella, placing the culture dish into a constant temperature incubator, culturing at 37 ℃ and saturated humidity for 24 hours, measuring the diameter (cm) of a bacteriostatic ring, wherein the larger the diameter of the bacteriostatic ring is, the better the bacteriostatic effect is. The results are shown in Table 1.
As can be seen from table 1, the traditional Chinese medicine powder with the mass concentration of 0.001g/mL has certain bacteriostatic activity, but the bacteriostatic circle is small and the bacteriostatic activity is weak; the antibacterial activity of the traditional Chinese medicine powder with the mass concentration of 0.01g/mL is obviously enhanced, and the size of the antibacterial zone of the traditional Chinese medicine powder prepared by the invention is obviously larger than that of the jiuyidan under the condition of the same concentration of medicine, which indicates that the antibacterial activity of the traditional Chinese medicine powder prepared by the invention is superior to that of the jiuyidan.
Thirdly, the situation of the wound of the rat to be healed and astringed
Wound healing refers to the healing process after the skin tissue is broken or damaged due to the action of external force. In the experiment, 12 healthy rabbits are used as test animals and are randomly divided into 3 groups of 4 rabbits each; an animal model is established by an operative cutting method, skin incisions are made on the backs of domestic rabbits, the left and right sides of the incision are 3 respectively by taking a spine as a boundary line, the wound area is about 2 multiplied by 2cm, and the rabbit is reserved after being washed by physiological saline.
The traditional Chinese medicine powder prepared in the embodiment 1 of the invention is used as a test group, the jiuyidan is used as a medicine control group, and the normal saline treatment group is used as a blank control group. Respectively adopting physiological saline, jiuyidan and the Chinese medicinal powder to carry out medicament treatment on the upper, middle and lower three wounds on the left and right sides of each rabbit; the wound convergence healing conditions were observed at 3d, 7d, 10d and 15d, respectively, and the healing rate was used as the healing index, and the test results were recorded and shown in table 2.
As can be seen from table 2, jiuyidan can promote wound healing compared with normal saline, and the healing condition of the traditional Chinese medicine powder prepared by the invention is that jiuyidan, which indicates that the traditional Chinese medicine powder prepared by the invention can promote wound healing, accelerate wound convergence, reduce tissue fluid exudation, keep the wound surface dry, reduce the degree of local wound edema, and form a protective film on the wound surface to prevent wound infection.
The invention has the beneficial effects that: the traditional Chinese medicine powder prepared by the invention is prepared according to the compatibility standard strictly, is safe and non-irritant, has antibacterial and bactericidal effects, has good antibacterial activity on staphylococcus aureus, pseudomonas aeruginosa and salmonella, and has an antibacterial effect obviously superior to that of jiuyidan. In the aspect of promoting wound healing, compared with the jiuyidan, the traditional Chinese medicine powder prepared by the invention can promote wound healing, accelerate wound convergence, reduce tissue fluid exudation, keep the wound surface dry and improve the wound healing quality; reducing local wound edema degree, forming a protective film on the wound surface to prevent wound infection, and has the effects of sterilizing and diminishing inflammation, relieving swelling and pain, promoting blood circulation by removing blood stasis, removing necrotic tissue and promoting granulation, and promoting wound healing.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1: the embodiment provides a traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing, which comprises 30g of borax, 30g of talcum, 15g of dried alum, 30g of Chinese ivy herb, 5g of realgar, 20g of myrrh, 80g of egg white, 30g of angelica dahurica, 20g of golden cypress, 2g of borneol, 5g of litharge, 40g of pseudo-ginseng, 30g of salvia miltiorrhiza, 100g of cactus and 100g of rosemary.
The preparation method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral powder, sieving the mineral powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder at 175 ℃ for 4 hours, and cooling the powder to room temperature for later use;
step two, rapidly freezing the egg white at-18 ℃ for 5 hours, freezing and then freeze-drying for 24 hours, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed, performing enzymolysis by adopting amylase, cellulase, pectinase and papain, then placing the obtained product into an aqueous solution, heating and distilling the obtained product for 0.5 to 1 hour to extract the obtained product to obtain rosemary perfume extract, adding n-hexane into the rosemary perfume extract to extract the obtained product for 2 to 3 times, merging the extract, filtering the obtained product, and removing the solvent to obtain rosemary essential oil;
respectively crushing and uniformly mixing radix angelicae, golden cypress, pseudo-ginseng, radix salviae miltiorrhizae, lacca mollissima and cactus, adding 8 times of 95% ethanol into the mixture, extracting for 60 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha arvensis essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
Example 2: the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises 40g of borax, 40g of talcum, 15g of dried alum, 30g of entomogenous medicine, 5g of realgar, 20g of myrrh, 100g of egg white, 30g of radix angelicae, 20g of golden cypress, 2g of borneol, 5g of litharge, 40g of pseudo-ginseng, 40g of salvia miltiorrhiza, 150g of cactus and 150g of rosemary.
The preparation method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral powder, sieving the mineral powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder at 175 ℃ for 4 hours, and cooling the powder to room temperature for later use;
step two, rapidly freezing the egg white at-18 ℃ for 5 hours, freezing and then freeze-drying for 24 hours, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed, performing enzymolysis by adopting amylase, cellulase, pectinase and papain, then placing the obtained product into an aqueous solution, heating and distilling the obtained product for 0.5 to 1 hour to extract the obtained product to obtain rosemary perfume extract, adding n-hexane into the rosemary perfume extract to extract the obtained product for 2 to 3 times, merging the extract, filtering the obtained product, and removing the solvent to obtain rosemary essential oil;
respectively crushing and uniformly mixing the angelica dahurica, the phellodendron amurense, the pseudo-ginseng, the salvia miltiorrhiza, the lacca-chard and the cactus, adding 10 times of 95% ethanol into the mixture to extract for 48 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha-elegans essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
Example 3: the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises 10g of borax, 50g of talcum, 10g of dried alum, 50g of entomogenous medicine, 10g of realgar, 10g of myrrh, 50g of egg white, 20g of radix angelicae, 30g of golden cypress, 1g of borneol, 2g of litharge, 30g of pseudo-ginseng, 20g of salvia miltiorrhiza, 150g of cactus and 50g of rosemary.
The preparation method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral powder, sieving the mineral powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder at 175 ℃ for 4 hours, and cooling the powder to room temperature for later use;
step two, rapidly freezing the egg white at-18 ℃ for 5 hours, freezing and then freeze-drying for 24 hours, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed, carrying out enzymolysis by adopting pectinase and papain, then placing the obtained product into an aqueous solution, heating and distilling the obtained product for 0.5-1h to extract the obtained product to obtain rosemary perfume extract, adding n-hexane into the rosemary perfume extract to extract the obtained product for 2-3 times, combining the extract, filtering the obtained product, and removing a solvent to obtain rosemary essential oil;
respectively crushing and uniformly mixing the angelica dahurica, the phellodendron amurense, the pseudo-ginseng, the salvia miltiorrhiza, the lacca-chard and the cactus, adding 10 times of 95% ethanol into the mixture to extract for 48 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha-elegans essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
Example 4: the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing comprises 50g of borax, 10g of talcum, 50g of dried alum, 10g of entomogenous medicine, 30g of realgar, 30g of myrrh, 100g of egg white, 40g of radix angelicae, 10g of golden cypress, 70g of borneol, 80g of litharge, 50g of pseudo-ginseng, 50g of salvia miltiorrhiza, 50g of cactus and 150g of rosemary.
The preparation method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral powder, sieving the mineral powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder at 175 ℃ for 4 hours, and cooling the powder to room temperature for later use;
step two, rapidly freezing the egg white at-18 ℃ for 5 hours, freezing and then freeze-drying for 24 hours, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed, performing enzymolysis by adopting cellulase and papain, then placing the mixture into an aqueous solution, heating and distilling the mixture for 0.5 to 1 hour to extract rosemary perfume extract, adding normal hexane into the rosemary perfume extract to extract for 2 to 3 times, merging the extract, filtering the extract, and removing the solvent to obtain rosemary essential oil;
respectively crushing and uniformly mixing the angelica dahurica, the phellodendron amurense, the pseudo-ginseng, the salvia miltiorrhiza, the lacca-chard and the cactus, adding 10 times of 95% ethanol into the mixture to extract for 48 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha-elegans essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
Application test
Selecting 120 white rats, randomly dividing into 4 groups, and establishing an animal model by a surgical cutting method, making skin incision on the backs of the rats, wherein the wound area is about 2 x 2cm, and washing with physiological saline for later use; tests 1-4 groups were treated with the formulations prepared in examples 1-4, respectively, and the healing rate = healing area/original wound area, with a healing rate of greater than 95% as complete healing and 70-95% as effective, was recorded after 10 days of continuous observation for 10 days, and the results are shown in table 3.
As can be seen from table 3, the total effective rates of the traditional Chinese medicine preparations prepared in examples 1 and 2 for rat wound healing are both greater than 95%, and the effective rates of the traditional Chinese medicine preparations prepared in examples 3 and 4 are slightly lower than that of example 1 but still higher than 90%; the traditional Chinese medicine preparation prepared by the invention has a good treatment effect on skin wounds and has the effects of sterilizing, diminishing inflammation and promoting wound healing.
The clinical application is as follows:
the applicant of the application can achieve the healing effect after taking the medicine for about 2 weeks by tracking the curative effect of 157 cases of skin injury and ulceration patients; tracing and revisiting 218 patients with beriberi, and taking the medicine for about 2-3 weeks without repeating; tracking the radial curative effect of 75 diabetic foot patients, and healing wound after 7-15 days of medication; see table 4 for details.
From table 4, it can be seen that the Chinese medicinal preparation has an excellent treatment effect on skin injury and ulceration, and the effective rate reaches 99%; the traditional Chinese medicine preparation can promote wound healing of diabetic feet, has a slightly weak effect on fungi but has an effective rate of more than 90%, and shows that the traditional Chinese medicine preparation can effectively treat skin injury, has bacteriostatic and bactericidal effects, can effectively promote wound healing, and has the curative effects of removing necrotic tissue and promoting granulation.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and scope of the present invention are intended to be covered thereby.
Claims (7)
1. A traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing is characterized in that: the feed comprises the following raw materials in parts by weight: 1-5 parts of borax, 1-5 parts of talcum, 1-2 parts of dried alum, 1-5 parts of entomogenous drug, 0.1-1 part of realgar, 1-3 parts of myrrh, 5-10 parts of egg white, 2-4 parts of angelica dahurica, 1-3 parts of phellodendron, 0.1-0.5 part of borneol, 0.2-0.5 part of lithargite, 3-5 parts of pseudo-ginseng, 2-5 parts of salvia miltiorrhiza, 5-15 parts of cactus and 5-15 parts of rosemary.
2. The Chinese medicinal preparation for sterilizing, diminishing inflammation and promoting wound healing according to claim 1, which is characterized in that: the feed comprises the following raw materials in parts by weight: 3 parts of borax, 3 parts of talcum, 1.5 parts of dried alum, 3 parts of Chinese ivy herb, 0.5 part of realgar, 2 parts of myrrh, 8 parts of egg white, 3 parts of angelica dahurica, 2 parts of phellodendron, 0.2 part of borneol, 0.5 part of lithargite, 4 parts of pseudo-ginseng, 3 parts of salvia miltiorrhiza, 10 parts of cactus and 10 parts of rosemary.
3. The Chinese medicinal preparation for sterilizing, diminishing inflammation and promoting wound healing according to claim 1, which is characterized in that: the feed comprises the following raw materials in parts by weight: 4 parts of borax, 4 parts of talcum, 1.5 parts of dried alum, 3 parts of Chinese ivy herb, 0.5 part of realgar, 2 parts of myrrh, 10 parts of egg white, 3 parts of angelica dahurica, 2 parts of phellodendron, 0.2 part of borneol, 0.3 part of litharge, 4 parts of pseudo-ginseng, 4 parts of salvia miltiorrhiza, 15 parts of cactus and 15 parts of rosemary.
4. A method for preparing the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing according to any one of claims 1 to 3, which is characterized in that: the method comprises the following steps:
step one, respectively crushing and grinding borax, dried alum, realgar, litharge, talc, borneol and myrrh to obtain mineral medicinal powder, sieving the mineral medicinal powder with a sieve of 80-100 meshes, placing the powder in a dry heat sterilization cabinet, sterilizing the powder for 3-5 hours at the temperature of 180 ℃, and cooling the powder to room temperature for later use after sterilization;
step two, rapidly freezing the egg white at-18 ℃ for 3-5h, freezing, freeze-drying for 12-24h, and crushing to obtain egg white freeze-dried powder;
step three, after the rosemary is crushed and broken, putting the rosemary into an aqueous solution, heating and distilling the rosemary for 0.5 to 1 hour to extract rosemary perfume extract, adding n-hexane into the rosemary perfume extract to extract for 2 to 3 times, combining the extract, filtering the combined extract, and removing the solvent to obtain rosemary essential oil;
respectively crushing and mixing radix angelicae, golden cypress, pseudo-ginseng, radix salviae miltiorrhizae, lacca mollissima and cactus, adding 5-10 times of 95% ethanol into the mixture, extracting for 48-72 hours to obtain an ethanol extract, concentrating the ethanol extract to remove the ethanol, adding the mentha haplocalyx essential oil into the concentrated solution after the ethanol is removed, and then freeze-drying to obtain traditional Chinese medicine freeze-dried powder;
step five, mixing the mineral powder, the egg white freeze-dried powder and the Chinese medicine freeze-dried powder uniformly to obtain the Chinese medicine preparation.
5. The preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing according to claim 4, is characterized in that: the wall breaking treatment method in the third step is enzymolysis.
6. The preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing according to claim 5, is characterized in that: the enzyme for enzymolysis comprises one or more of amylase, cellulase, pectinase and papain.
7. The preparation method of the traditional Chinese medicine preparation for sterilizing, diminishing inflammation and promoting wound healing according to claim 4, is characterized in that: in the fourth step, 8 times of 95% ethanol is added into the mixture for extraction for 60 h.
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