CN112226382A - Microbial agent for inhibiting hydrogen sulfide generated in straw returning process and application thereof - Google Patents
Microbial agent for inhibiting hydrogen sulfide generated in straw returning process and application thereof Download PDFInfo
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- CN112226382A CN112226382A CN202010976298.5A CN202010976298A CN112226382A CN 112226382 A CN112226382 A CN 112226382A CN 202010976298 A CN202010976298 A CN 202010976298A CN 112226382 A CN112226382 A CN 112226382A
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- 239000010902 straw Substances 0.000 title claims abstract description 66
- 230000000813 microbial effect Effects 0.000 title claims abstract description 57
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 49
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229910000037 hydrogen sulfide Inorganic materials 0.000 title claims abstract description 33
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 98
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 60
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 42
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 42
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 39
- 235000009566 rice Nutrition 0.000 claims abstract description 39
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 31
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 30
- 239000004310 lactic acid Substances 0.000 claims abstract description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 28
- 235000013312 flour Nutrition 0.000 claims abstract description 28
- 239000008103 glucose Substances 0.000 claims abstract description 28
- 239000002689 soil Substances 0.000 claims abstract description 18
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 17
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 230000019086 sulfide ion homeostasis Effects 0.000 claims abstract description 6
- 241000209094 Oryza Species 0.000 claims description 37
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 30
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 13
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 13
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 13
- 241000187708 Micromonospora Species 0.000 claims description 8
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 8
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 8
- 241000187395 Streptomyces microflavus Species 0.000 claims description 8
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 7
- 241000186660 Lactobacillus Species 0.000 claims description 7
- 229940039696 lactobacillus Drugs 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 238000000354 decomposition reaction Methods 0.000 abstract description 8
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052717 sulfur Inorganic materials 0.000 abstract description 5
- 239000011593 sulfur Substances 0.000 abstract description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 4
- 230000001988 toxicity Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract 2
- 241000209140 Triticum Species 0.000 description 8
- 235000021307 Triticum Nutrition 0.000 description 8
- 240000008042 Zea mays Species 0.000 description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 8
- 235000005822 corn Nutrition 0.000 description 8
- 238000007789 sealing Methods 0.000 description 6
- 239000007789 gas Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000010907 stover Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- C12N1/18—Baker's yeast; Brewer's yeast
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Abstract
The invention discloses a microbial agent for inhibiting hydrogen sulfide generation in straw returning and application thereof. The microbial agent is prepared by mixing photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose. Wherein the components by weight are as follows: 50-60 parts of photosynthetic bacteria, 20-30 parts of actinomycetes, 20-25 parts of lactic acid bacteria, 10-15 parts of yeast, 2-5 parts of rice flour and 1-5 parts of glucose. When returning the straws to the field, the microbial inoculum is 15kg-30kg/hm2The dosage of the microbial inoculum is uniformly scattered on the straws, then the microbial inoculum and the straws are turned into soil, and then water is irrigated into the field. When the invention is used for returning straws to the field in a paddy field, a large amount of beneficial microorganisms can be rapidly bred, sulfur-containing organic matters generated in the straw decomposition process are fully utilized, the generation of hydrogen sulfide in the straw decomposition process is inhibited, the toxicity of the hydrogen sulfide to the root system of crops in the paddy field is reduced, and the utilization rate of the straws is improved.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a microbial agent for inhibiting hydrogen sulfide generation in straw returning and application thereof.
Background
Returning straws to the field is a method for applying straws (such as wheat straws, corn straws, rice straws and the like) which are not suitable for being directly used as feed to soil directly or after being piled up and decomposed. In a paddy field where straws are directly returned to the field, the straws start to rot greatly 7-10 days after water is supplied, oxygen in soil is consumed by the rotten straws, and oxygen deficiency of the soil in the field can be caused under the condition that water exists on the field surface, so that sulfur-containing organic matters in the straws are decomposed to generate hydrogen sulfide. The hydrogen sulfide has strong toxic action on the root system of paddy field crops (such as rice), the damaged seedling root system is yellow, the root system becomes black and rot in severe cases, and leaves are yellow and withered.
Disclosure of Invention
The invention aims to provide a microbial agent for inhibiting hydrogen sulfide generation in straw returning and application thereof, fully utilizes sulfur-containing organic matters in the straw, inhibits the generation of hydrogen sulfide in the straw rotting process, and reduces the toxicity of the hydrogen sulfide to paddy field crops.
On the one hand, the invention provides a microbial agent for inhibiting hydrogen sulfide generation in straw returning, which is prepared by mixing photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose.
Preferably, the microbial agent is prepared by mixing 50-60 parts by weight of the photosynthetic bacteria, 20-30 parts by weight of the actinomycetes, 20-25 parts by weight of the lactic acid bacteria, 10-15 parts by weight of the yeast, 2-5 parts by weight of the rice flour and 1-5 parts by weight of the glucose.
Preferably, the microbial agent is prepared by mixing 50 parts by weight of the photosynthetic bacteria, 30 parts by weight of the actinomycetes, 23 parts by weight of the lactic acid bacteria, 15 parts by weight of the yeast, 5 parts by weight of the rice flour and 5 parts by weight of the glucose.
Preferably, the microbial agent is prepared by mixing 54 parts by weight of photosynthetic bacteria, 27 parts by weight of actinomycetes, 25 parts by weight of lactic acid bacteria, 13 parts by weight of yeast, 4 parts by weight of rice flour and 3 parts by weight of glucose.
Preferably, the microbial agent is prepared by mixing 57 parts by weight of the photosynthetic bacteria, 24 parts by weight of the actinomycetes, 20 parts by weight of the lactic acid bacteria, 10 parts by weight of the yeast, 3 parts by weight of the rice flour and 4 parts by weight of the glucose.
Preferably, the microbial agent is prepared by mixing 60 parts by weight of the photosynthetic bacteria, 20 parts by weight of the actinomycetes, 21 parts by weight of the lactic acid bacteria, 11 parts by weight of the yeast, 2 parts by weight of the rice flour and 1 part by weight of the glucose.
Preferably, the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, the actinomycetes comprise micromonospora and streptomyces microflavus, the lactic acid bacteria comprise lactobacillus plantarum and lactobacillus paracasei, and the yeasts comprise saccharomyces cerevisiae and candida utilis.
Preferably, the bacterial content of the photosynthetic bacteria is more than or equal to 1.0 x 1010cfu/g, the bacterium content of the actinomycetes is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of the lactobacillus is more than or equal to 1.0 multiplied by 1010cfu/g, the bacteria content of the microzyme is more than or equal to 5.0 multiplied by 109cfu/g。
On the other hand, the invention provides the application of the microbial agent, the microbial agent is uniformly scattered on the straws when the straws are returned to the field, then the microbial agent and the straws are turned into soil, and then water is poured into the field.
Preferably, the dosage of the microbial agent is 15kg-30kg/hm2。
The microbial agent disclosed by the invention is applied to straw returning in paddy fields, can rapidly reproduce a large amount of beneficial microorganisms, fully utilizes sulfur-containing organic matters generated in the straw decomposition process, inhibits the generation of hydrogen sulfide generated in the straw decomposition process, reduces the toxicity of the hydrogen sulfide to the roots of crops in paddy fields, and improves the utilization rate of the straws.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention prepares a microbial agent by mixing photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose according to a certain proportion. The method is applied to returning straws to the field in the paddy field, can quickly breed a large number of beneficial microorganisms, fully utilizes sulfur-containing organic matters generated in the straw decomposition process, inhibits the generation of hydrogen sulfide, reduces the toxicity of the hydrogen sulfide to the root systems of crops in the paddy field, and improves the utilization rate of the straws. The microbial agent preferably comprises 50-60 parts by weight of photosynthetic bacteria, 20-30 parts by weight of actinomycetes, 20-25 parts by weight of lactic acid bacteria, 10-15 parts by weight of yeast, 2-5 parts by weight of rice flour and 1-5 parts by weight of glucose. Wherein the photosynthetic bacteria preferably comprise Rhodopseudomonas palustris and Rhodospirillum rubrum, and the bacteria content of both photosynthetic bacteria is not less than 1.0 × 1010cfu/g; the actinomycetes preferably include Micromonospora and Streptomyces microflavus, and the bacteria content of both actinomycetes is more than or equal to 1.0 × 109cfu/g; the lactobacillus preferably comprises Lactobacillus plantarum and Lactobacillus paracasei, and the bacterial content of both lactobacillus plantarum and Lactobacillus paracasei is more than or equal to 1.0 × 1010cfu/g; the yeast preferably comprises Saccharomyces cerevisiae and Candida utilis, and the bacteria content of both yeasts is not less than 1.0 × 1010cfu/g. The bacteria can be prepared by fermenting rhodopseudomonas palustris, rhodospirillum rubrum, micromonospora, streptomyces microflavus, lactobacillus plantarum, lactobacillus paracasei, saccharomyces cerevisiae and candida utilis in corresponding liquid culture media to obtain bacterial liquids, separating the bacterial bodies from the fermented culture, drying and concentrating to prepare single-strain solid stateAnd (4) fungus powder.
According to one embodiment of the present invention, the microbial agent comprises 50 parts of photosynthetic bacteria, 30 parts of actinomycetes, 23 parts of lactic acid bacteria, 15 parts of yeast, 5 parts of rice flour and 5 parts of glucose by weight.
According to one embodiment of the present invention, the microbial agent comprises 54 parts of photosynthetic bacteria, 27 parts of actinomycetes, 25 parts of lactic acid bacteria, 13 parts of yeast, 4 parts of rice flour and 3 parts of glucose by weight.
According to one embodiment of the present invention, the microbial agent comprises 57 parts of photosynthetic bacteria, 24 parts of actinomycetes, 20 parts of lactic acid bacteria, 10 parts of yeast, 3 parts of rice flour and 4 parts of glucose by weight.
According to one embodiment of the present invention, the microbial agent comprises 60 parts of photosynthetic bacteria, 20 parts of actinomycetes, 21 parts of lactic acid bacteria, 11 parts of yeast, 2 parts of rice flour and 1 part of glucose by weight.
The invention also provides the application of any microbial agent, when returning the straws to the field, the microbial agent is uniformly scattered on the straws on the basis of conventional operation, then the microbial agent and the straws are turned into soil, and water is poured into the field to ensure that the water surface is 3-5cm higher than the soil. The dosage of the microbial agent is preferably 15-30 kg/hm2。
To help better understand the technical solution of the present invention, the following examples are provided for illustrating the microbial agent of the present invention and the preparation process thereof.
The first embodiment is as follows: preparation of microbial agent 1
The raw materials by weight ratio are as follows: 50 parts of photosynthetic bacteria, 30 parts of actinomycetes, 23 parts of lactic acid bacteria, 15 parts of saccharomycetes, 5 parts of rice flour and 5 parts of glucose, wherein the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, and the bacteria content of the two photosynthetic bacteria is more than or equal to 1.0 multiplied by 1010cfu/g; the actinomycetes include Micromonospora and Streptomyces microflavus, and the bacteria content of both actinomycetes is more than or equal to 1.0 × 109cfu/g; the lactobacillus comprises Lactobacillus plantarum and Lactobacillus paracasei, and the bacteria content of both lactobacillus plantarum and Lactobacillus paracasei is more than or equal to 1.0 × 1010cfu/g; the yeast includes Saccharomyces cerevisiae and Candida utilisThe bacteria content of the two yeasts is more than or equal to 1.0 multiplied by 1010cfu/g。
Uniformly stirring photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose to obtain the microbial agent 1.
Example two: preparation of microbial agent 2
The raw materials by weight ratio are as follows: 54 parts of photosynthetic bacteria, 27 parts of actinomycetes, 25 parts of lactic acid bacteria, 13 parts of saccharomycetes, 4 parts of rice flour and 3 parts of glucose, wherein the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, and the bacteria content of the two photosynthetic bacteria is more than or equal to 1.0 multiplied by 1010cfu/g; the actinomycetes include Micromonospora and Streptomyces microflavus, and the bacteria content of both actinomycetes is more than or equal to 1.0 × 109cfu/g; the lactobacillus comprises Lactobacillus plantarum and Lactobacillus paracasei, and the bacteria content of both lactobacillus plantarum and Lactobacillus paracasei is more than or equal to 1.0 × 1010cfu/g; the yeast comprises Saccharomyces cerevisiae and Candida utilis, and the bacteria content of the two yeasts is not less than 1.0 × 1010cfu/g。
Uniformly stirring photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose to obtain the microbial agent 2.
Example three: preparation of microbial agent 3
The raw materials by weight ratio are as follows: 57 parts of photosynthetic bacteria, 24 parts of actinomycetes, 20 parts of lactic acid bacteria, 10 parts of saccharomycetes, 3 parts of rice flour and 4 parts of glucose, wherein the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, and the bacteria content of the two photosynthetic bacteria is more than or equal to 1.0 multiplied by 1010cfu/g; the actinomycetes include Micromonospora and Streptomyces microflavus, and the bacteria content of both actinomycetes is more than or equal to 1.0 × 109cfu/g; the lactobacillus comprises Lactobacillus plantarum and Lactobacillus paracasei, and the bacteria content of both lactobacillus plantarum and Lactobacillus paracasei is more than or equal to 1.0 × 1010cfu/g; the yeast comprises Saccharomyces cerevisiae and Candida utilis, and the bacteria content of the two yeasts is not less than 1.0 × 1010cfu/g。
Uniformly stirring photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose to obtain the microbial agent 3.
Example four: microbial strainsPreparation of agent 4
The raw materials by weight ratio are as follows: 60 parts of photosynthetic bacteria, 20 parts of actinomycetes, 21 parts of lactic acid bacteria, 11 parts of saccharomycetes, 2 parts of rice flour and 1 part of glucose, wherein the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, and the bacteria content of the two photosynthetic bacteria is more than or equal to 1.0 multiplied by 1010cfu/g; the actinomycetes include Micromonospora and Streptomyces microflavus, and the bacteria content of both actinomycetes is more than or equal to 1.0 × 109cfu/g; the lactobacillus comprises Lactobacillus plantarum and Lactobacillus paracasei, and the bacteria content of both lactobacillus plantarum and Lactobacillus paracasei is more than or equal to 1.0 × 1010cfu/g; the yeast comprises Saccharomyces cerevisiae and Candida utilis, and the bacteria content of the two yeasts is not less than 1.0 × 1010cfu/g。
Photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose are uniformly stirred to obtain the microbial agent 4.
In order to help better understand the technical scheme of the invention, a straw returning test in a paddy field is provided below for explaining the application method of the invention and the influence of the application method on the hydrogen sulfide generated when the straw is returned to the field.
Test one: influence of microbial inoculum on hydrogen sulfide generation in returning straws to field in paddy field
The method of returning the straws to the field directly is adopted to carry out the returning test of the rice straws, the corn straws and the wheat straws in the same rice field. The basic physical and chemical properties of the field soil are that the pH value is 7.87, the organic matter is 22.27g/kg, the total nitrogen is 1.31g/kg, the total phosphorus is 0.42g/kg, and the total potassium is 25.31 g/kg. The total number of the tests is 15, each straw is 5, each group of the tests is designed with 3 test cells, and the area of each test cell is 10m2All test cells are randomly distributed, and the test period is 30 d. After the rice is harvested, the fields in all the districts are cleared up, and then a straw returning test is carried out.
In the 1-5 group experiments, the rice straws are firstly crushed into small sections with the length of about 10cm, and then 9000kg/hm of the small sections is added2And flatly paving the crushed rice straws on the soil surface of each cell. The control group does not apply the microbial inoculum, and the test group is 15kg/hm2The prepared microbial agent is uniformly scattered on the rice straws in the using amountA surface. Then the microbial inoculum and the rice straws are simultaneously turned into the soil, water is irrigated into the field to ensure that the water surface is 5cm higher than the soil surface, and each cell is independently built with a plastic shed for sealing. The hydrogen sulfide concentration in the plastic sheds of the cells is measured by a hydrogen sulfide gas detector every 5 days from the completion of the sealing of the test cells, and the average concentration (ppm) of hydrogen sulfide gas in each group of paddy fields in different time is counted after the test is finished, and the result is shown in table 1.
TABLE 1
As can be seen from the data in Table 1, the concentrations of hydrogen sulfide generated during the decomposition process of four groups of rice straws applied with the microbial agent are obviously lower than those of the control group. Therefore, the prepared microbial inoculum 1-4 can obviously inhibit the generation of hydrogen sulfide in the process of returning rice straws to the field in the paddy field.
In the 6-10 group test, the corn stalks are firstly crushed into small sections with the length of about 10cm, and then 9000kg/hm of the small sections is added2The crushed corn straws are paved on the soil surface of each district. The control group does not apply the microbial inoculum, and the test group is 23kg/hm2The prepared microbial agent is uniformly scattered on the surface of the corn straws according to the using amount. Then, the microbial inoculum and the corn straws are simultaneously turned into soil, water is irrigated into the field to enable the water surface to be 5cm higher than the soil surface, and each cell is independently built with a plastic shed for sealing. The hydrogen sulfide concentration in the plastic sheds of the cells is measured by a hydrogen sulfide gas detector every 5 days from the completion of the sealing of the test cells, and the average concentration (ppm) of hydrogen sulfide gas in each group of paddy fields in different time is counted after the test is finished, and the result is shown in table 2.
TABLE 2
As can be seen from the data in Table 2, the concentrations of hydrogen sulfide produced during the decomposition process were significantly lower in the four groups of corn stover applied with the microbial inoculum than in the control group. Therefore, the prepared microbial inoculum 1-4 can obviously inhibit the generation of hydrogen sulfide in the process of returning corn straws to the field in the paddy field.
In the 11-15 group tests, the wheat straws are firstly crushed into small sections with the length of about 10cm, and then 9000kg/hm of the small sections is added2And flatly paving the crushed wheat straws on the soil surface of each cell. The control group does not apply the microbial inoculum, and the test group is 30kg/hm2The prepared microbial agent is uniformly scattered on the surface of the wheat straw. Then, the microbial inoculum and the wheat straws are simultaneously turned into soil, water is irrigated into the field to enable the water surface to be 5cm higher than the soil surface, and each cell is independently built with a plastic shed for sealing. The hydrogen sulfide concentration in the plastic sheds of the cells was measured every 5 days from the completion of the sealing of the test cells, and the average hydrogen sulfide concentration (ppm) in each group of paddy fields was counted at different times after the completion of the test, with the results shown in table 3.
TABLE 3
As can be seen from the data in Table 3, the concentration of hydrogen sulfide generated during the decomposition process of the four groups of wheat straws applied with the microbial agent is obviously lower than that of the control group. Therefore, the prepared microbial inoculum 1-4 can obviously inhibit the generation of hydrogen sulfide in the process of returning wheat straws to the field in the paddy field.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (10)
1. A microbial agent for inhibiting hydrogen sulfide generation in straw returning is characterized in that: the microbial agent is prepared by mixing photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, rice flour and glucose.
2. The microbial inoculant according to claim 1, wherein: the microbial agent is prepared by mixing 50-60 parts by weight of photosynthetic bacteria, 20-30 parts by weight of actinomycetes, 20-25 parts by weight of lactic acid bacteria, 10-15 parts by weight of saccharomycetes, 2-5 parts by weight of rice flour and 1-5 parts by weight of glucose.
3. The microbial inoculant according to claim 2, wherein: the microbial agent is prepared by mixing 50 parts by weight of photosynthetic bacteria, 30 parts by weight of actinomycetes, 23 parts by weight of lactic acid bacteria, 15 parts by weight of saccharomycetes, 5 parts by weight of rice flour and 5 parts by weight of glucose.
4. The microbial inoculant according to claim 2, wherein: the microbial agent is prepared by mixing 54 parts by weight of photosynthetic bacteria, 27 parts by weight of actinomycetes, 25 parts by weight of lactic acid bacteria, 13 parts by weight of saccharomycetes, 4 parts by weight of rice flour and 3 parts by weight of glucose.
5. The microbial inoculant according to claim 2, wherein: the microbial agent is prepared by mixing 57 parts by weight of photosynthetic bacteria, 24 parts by weight of actinomycetes, 20 parts by weight of lactic acid bacteria, 10 parts by weight of saccharomycetes, 3 parts by weight of rice flour and 4 parts by weight of glucose.
6. The microbial inoculant according to claim 2, wherein: the microbial agent is prepared by mixing 60 parts by weight of photosynthetic bacteria, 20 parts by weight of actinomycetes, 21 parts by weight of lactic acid bacteria, 11 parts by weight of saccharomycetes, 2 parts by weight of rice flour and 1 part by weight of glucose.
7. The microbial inoculant according to any one of claims 1 to 6, wherein: the photosynthetic bacteria comprise rhodopseudomonas palustris and rhodospirillum rubrum, the actinomycetes comprise micromonospora and streptomyces microflavus, the lactic acid bacteria comprise lactobacillus plantarum and lactobacillus paracasei, and the yeasts comprise saccharomyces cerevisiae and candida utilis.
8. The microbial inoculant according to any one of claims 1 to 6, wherein: the bacterial content of the photosynthetic bacteria is more than or equal to 1.0 multiplied by 1010cfu/g, the bacterium content of the actinomycetes is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of the lactobacillus is more than or equal to 1.0 multiplied by 1010cfu/g, the bacteria content of the microzyme is more than or equal to 5.0 multiplied by 109cfu/g。
9. Use of a microbial inoculant according to any one of claims 1 to 8, wherein: and when returning the straws to the field, uniformly scattering the microbial inoculum on the straws, then turning the microbial inoculum and the straws into soil, and irrigating the field.
10. Use according to claim 9, characterized in that: the dosage of the microbial agent is 15kg-30kg/hm2。
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