CN112190686A - 一种畜禽补气血提高免疫力的中药酵素及其制备方法 - Google Patents
一种畜禽补气血提高免疫力的中药酵素及其制备方法 Download PDFInfo
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Abstract
本发明公开了一种畜禽补气血提高免疫力的中药酵素及其制备方法,本发明以生姜、大枣、枸杞、山楂、草果、黄瓜、苹果、胡萝卜、青果、熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花为原料,制备得到的中药酵素可改善饲料品质,提高禽畜免疫力和成活率,促进消化吸收,提高日增重,加快生长速度,提高饲料利用率,降低饲养成本。本发明的中药酵素中富含有益酶、菌体蛋白和代谢产物,能够提高饲料的适口性好。
Description
技术领域
本发明属于中药酵素领域,特别涉及一种畜禽补气血提高免疫力的中药酵素及其制备方法。
背景技术
随着集约化、规模化养殖的发展,养殖动物疾病肆虐,造成严重的经济损失和社会危害。目前,尽管疫苗作为防控传染性疾病手段得到了政府、企业和养殖户的重视,在防控疫病方面做出了不可磨灭的贡献,但是其存在的缺陷也是众所周知的。中兽医学认为畜禽体与自然环境密切相关,“阴平阳秘、精神乃治”,重在平衡阴阳;“正气内存、邪不可干”,重在提高机体的非特异性免疫能力。而现有禽畜养殖业的惯用手法是在饲料中增加化学或人工合成的化工、兽药等各种添加剂,这些添加剂对各种牲畜、家禽都有较大的副作用,甚至含有较多的药品、毒素残留物,不仅存在食品质量安全隐患,而且影响食用者的身体健康
发明内容
为了解决上述技术问题,本发明提供了一种畜禽补气血提高免疫力的中药酵素及其制备方法。
本发明的技术方案是这样实现的:
一种畜禽补气血提高免疫力的中药酵素,中药由复合菌发酵而成,中药包括以下重量份数的原料:
生姜20-30份、大枣30-50份、枸杞10-15份、熟地黄10-15份、当归5-15份、白芍5-15份、川芎4-8份、人参5-15份、白术5-15份、茯苓5-15份、炙甘草3-8份、黄芪10-15份、肉桂1-5份、益母草3-8份、半夏3-8份、骨碎补5-15份、决明子3-8份、百合2-3份、金银花3-8份、微藻粉3-5份、糖30-50份、200-500份山楂、200-500份草果、黄瓜1000-2000份、苹果2000-3000份、胡萝卜2000-3000份、青果100-200份。
进一步的,复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌。
进一步的,双歧杆菌的活菌数为5×108-8×108CFU/ml,醋酸杆菌的活菌数为6×107-10×107CFU/ml,植物乳杆菌的活菌数为1×109-2×109CFU/ml,枯草芽孢杆菌的活菌数为3×106-5×106CFU/ml,米曲霉的活菌数为3×105-5×105CFU/ml,乳酸菌的活菌数为4×108-10×108CFU/ml。
进一步的,微藻粉为螺旋藻粉或小球藻粉。
本发明还提供了上述一种畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2-3h,冷却至20~25℃,得到混合液,备用;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在25~35mg/L,发酵温度18~20℃,搅拌速度5~15r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为10~30目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于45-55℃下酶解10-30min;再超声处理10~15min;再加入碱性物质,将溶液的PH值调节至6~7,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-10℃-0℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为600~800目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、微藻粉、糖加入到粗发酵液中,在40-50℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在10~15℃,得到畜禽补气血提高免疫力的中药酵素。
进一步的,还包括将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
本发明提出了一种畜禽补气血提高免疫力的中药酵素及其制备方法,由补气补血(生姜、大枣、枸杞)、四物汤(熟地、当归、白芍、川芎),四君子汤(人参、白术、茯苓、炙甘草)等组成,制备得到的中药酵素可改善饲料品质,提高禽畜免疫力和成活率,促进消化吸收,提高日增重,加快生长速度,提高饲料利用率,降低饲养成本。
具体实施方式
一种畜禽补气血提高免疫力的中药酵素,中药由复合菌发酵而成,中药包括以下重量份数的原料:
生姜20-30份、大枣30-50份、枸杞10-15份、熟地黄10-15份、当归5-15份、白芍5-15份、川芎4-8份、人参5-15份、白术5-15份、茯苓5-15份、炙甘草3-8份、黄芪10-15份、肉桂1-5份、益母草3-8份、半夏3-8份、骨碎补5-15份、决明子3-8份、百合2-3份、金银花3-8份、微藻粉3-5份、糖30-50份、山楂200-500份、草果200-500份、黄瓜1000-2000份、苹果2000-3000份、胡萝卜2000-3000份、青果100-200份。
本发明由补气补血(生姜、大枣、枸杞)、四物汤(熟地、当归、白芍、川芎),四君子汤(人参、白术、茯苓、炙甘草)等组成,制备得到的中药酵素可改善饲料品质,提高禽畜免疫力和成活率,促进消化吸收,提高日增重,加快生长速度,提高饲料利用率,降低饲养成本。
生姜起到镇吐、活血驱寒的作用。生姜的重量份数为20-30份;优选地,生姜的重量份数为24-27份。
大枣起到健脾益气,养阴血,调和药性,固护脾胃的功效。大枣的重量份数为30-50份;优选地,大枣的重量份数为35-45份。
枸杞起到养肝,滋肾,润肺的功效。枸杞的重量份数为10-15份;优选地,枸杞的重量份数为12-13份。
熟地黄起到清热凉血、养阴生津的作用。熟地黄的重量份数为10-15份;优选地,熟地黄的重量份数为12-13份。
当归起到润肠通便、活血化瘀、调经止痛的功效。当归的重量份数为5-15份;优选地,当归的重量份数为8-12份。
白芍起到养血调经,敛阴止汗,柔肝止痛,平抑肝阳的作用。白芍的重量份数为5-15份;优选地,白芍的重量份数为8-12份。
川芎起到活血祛瘀、行气开郁、祛风止痛的作用。川芎的重量份数为4-8份;优选地,川芎的重量份数为5-7份。
人参具有大补元气、复脉固脱、补脾益肺、安神益智的功效。人参的重量份数为5-15份;优选地,人参的重量份数为8-12份。
白术具有健脾益气、燥湿利水、止汗的功效。白术的重量份数为5-15份;优选地,白术的重量份数为8-12份。
茯苓具有利水渗湿,健脾,宁心的作用。茯苓的重量份数为5-15份;优选地,茯苓的重量份数为8-12份。
炙甘草具有滋阴养血,益气通阳,复脉定悸的作用。炙甘草的重量份数为3-8份;优选地,炙甘草的重量份数为5-7份。
黄芪具有补气、止汗、利尿消肿、排脓的作用。黄芪的重量份数为10-15份;优选地,黄芪的重量份数为12-13份。
肉桂具有镇静镇痛、降温、抗菌、通经的作用。肉桂的重量份数为1-5份;优选地,肉桂的重量份数为2-4份。
益母草具有活血调经、利尿消肿、清热解毒的作用。益母草的重量份数为3-8份;优选地,益母草的重量份数为5-6份。
半夏起到燥湿化痰、和胃止呕的作用。半夏的重量份数为3-8份;优选地,半夏的重量份数为5-6份。
骨碎补具有补肾强骨、续伤止痛的作用。骨碎补的重量份数为5-15份;优选地,骨碎补的重量份数为8-12份。
决明子具有降压、抗菌和降低胆固醇的作用。决明子的重量份数为3-8份;优选地,决明子的重量份数为5-6份。
百合具有养阴清热,滋补精的作用。百合的重量份数为2-3份。
金银花具有清热解毒、疏散风热、凉血止痢的作用。金银花的重量份数为3-8份;优选地,金银花的重量份数为5-6份。
微藻粉起到增强免疫力,促进食欲的作用。微藻粉的重量份数为3-5份;优选地,微藻粉的重量份数为3.5-4.5份。
需要说明的是,微藻粉为螺旋藻粉或小球藻粉。
糖的重量份数为30-50份;优选地,糖的重量份数为35-45份。
山楂具有消食健胃、活血化淤、收敛止痢的作用。山楂的重量份数为200-500份;优选地,山楂的重量份数为300-400份。
草果具有燥湿除寒、行气止痛、祛痰截疟、消食化积的作用。草果的重量份数为200-500份;优选地,草果的重量份数为300-400份。
黄瓜具有清热解毒,健脑安神的作用。黄瓜的重量份数为1000-2000份;优选地,黄瓜的重量份数为1400-1700份。
苹果具有补脑养血、宁神安眠的作用。苹果的重量份数为2000-3000份;优选地,苹果的重量份数为2400-2600份。
胡萝卜具有补肝明目,清热解毒的作用。胡萝卜的重量份数为2000-3000份;优选地,胡萝卜的重量份数为2400-2600份。
青果具有清热解毒、利咽化痰、降血糖的作用。青果的重量份数为100-200份;优选地,青果的重量份数为130-160份。
在本发明中,复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌。优选地,双歧杆菌的活菌数为5×108-8×108CFU/ml,醋酸杆菌的活菌数为6×107-10×107CFU/ml,植物乳杆菌的活菌数为1×109-2×109CFU/ml,枯草芽孢杆菌的活菌数为3×106-5×106CFU/ml,米曲霉的活菌数为3×105-5×105CFU/ml,乳酸菌的活菌数为4×108-10×108CFU/ml。上述混合菌剂协同发酵,中药成分充分降解,微生物发酵后的中药有害杂质可以得到更充分的去除,大分子难于吸收的成分被微生物降解为小分子活性物质,同时,发酵液中富含有益酶、菌体蛋白和代谢产物,能够提高饲料的适口性好。
本发明还提供了上述一种畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2-3h,冷却至20~25℃,得到混合液,备用;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在25~35mg/L,发酵温度18~20℃,搅拌速度5~15r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为10~30目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于45-55℃下酶解10-30min;再超声处理10~15min;再加入碱性物质,将溶液的PH值调节至6~7,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-10℃-0℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为600~800目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、微藻粉、糖加入到粗发酵液中,在40-50℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在10~15℃,得到畜禽补气血提高免疫力的中药酵素。
其中,生姜、大枣、枸杞、山楂、草果、黄瓜、苹果、胡萝卜、青果、熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花均同上所述,在此不再赘述。
本发明中生姜、大枣、枸杞进行熬煮,能够将其中的有效成分萃取出来;山楂、草果、黄瓜、苹果、胡萝卜、青果经过复合菌发酵而产生的,含有丰富的酶、维生素、矿物质和次生代谢产物;生姜、大枣、枸杞、熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花等中药原料先进行破壁粉碎,达到破碎细胞形态水平,胞内有效物质得以充分释放,更易被吸收,利用率更高;再将混合液、粗发酵液和破壁粉进行发酵过程可显著提高中药有效成分的浸出率,同时能够提高饲料的适口性。
需要说明的是,还包括将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
为了进一步说明本发明,下面结合实施例对本发明提供的一种畜禽补气血提高免疫力的中药酵素及其制备方法进行详细地描述,但不能将它们理解为对本发明保护范围的限定。
实施例1
畜禽补气血提高免疫力的中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜20份、大枣30份、枸杞10份、熟地黄10份、当归5份、白芍5份、川芎4份、人参5份、白术5份、茯苓5份、炙甘草3份、黄芪10份、肉桂1份、益母草3份、半夏3份、骨碎补5份、决明子3份、百合2份、金银花3份、螺旋藻粉3份、糖30份、200份山楂、200份草果、黄瓜10000份、苹果2000份、胡萝卜2000份、青果100份。
复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌,双歧杆菌的活菌数为5×108CFU/ml,醋酸杆菌的活菌数为6×107CFU/ml,植物乳杆菌的活菌数为1×109CFU/ml,枯草芽孢杆菌的活菌数为3×106CFU/ml,米曲霉的活菌数为3×105CFU/ml,乳酸菌的活菌数为4×108CFU/ml。
畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2h,冷却至20℃,得到混合液,备用;其中,水的加入量是生姜、打造、枸杞总质量的10倍;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在25mg/L,发酵温度18℃,搅拌速度5r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为10目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于45℃下酶解10min;再超声处理10min;再加入碱性物质,将溶液的PH值调节至6,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-10℃℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为600目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、螺旋藻粉、糖加入到粗发酵液中,在40℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在10℃,得到畜禽补气血提高免疫力的中药酵素;
6)将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
将上述畜禽补气血提高免疫力的中药酵素加入肉猪饲料,加入量为饲料重量的1%,混合均匀,配好后与未加发酵饲料的肉猪饲料进行对照试验。试验猪选择长白种猪60头,猪,选择猪体生长发育正常,健康无病,初生期的仔猪,随机分为试验组1和对照组,两组的品种相同,出生日期和体重基本一致。
试验在河北某养殖基地进行,试验期30d。现场试验严格按照猪场常规养殖管理进行,分别在07:00、10:00、13:30和15:30对各组仔猪进行拌料饲喂。
在第1天和第30天,对猪进行空腹称重,计算平均日增重。每天记录各组采食量,试验结束,结算各组耗料量,计算平均日采食量和料重比,每天08:00观察猪粪便情况,记录每组仔猪日腹泻头数,计算腹泻率:腹泻率(%)=100×腹泻头数/(试验猪头数×试验天数),结果见表1。
表1试验结果
由上表可知,采用本发明中药酵素的长白种猪平均日增重达724g,比对照组的日增重0.683g提高了6.0%;料肉比从2.90提高到了273,饲料转化率提高了6.23%。
实施例2
中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜30份、大枣50份、枸杞15份、熟地黄15份、当归15份、白芍15份、川芎8份、人参15份、白术15份、茯苓15份、炙甘草8份、黄芪15份、肉桂5份、益母草8份、半夏8份、骨碎补15份、决明子8份、百合3份、金银花8份、球藻粉5份、糖50份、200-500份山楂、500份草果、黄瓜2000份、苹果3000份、胡萝卜3000份、青果200份。
复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌,双歧杆菌的活菌数为8×108CFU/ml,醋酸杆菌的活菌数为10×107CFU/ml,植物乳杆菌的活菌数为2×109CFU/ml,枯草芽孢杆菌的活菌数为5×106CFU/ml,米曲霉的活菌数为5×105CFU/ml,乳酸菌的活菌数为10×108CFU/ml。
畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮3h,冷却至25℃,得到混合液,备用;其中,水的加入量是生姜、打造、枸杞总质量的10倍;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在35mg/L,发酵温度20℃,搅拌速度15r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为30目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于55℃下酶解30min;再超声处理15min;再加入碱性物质,将溶液的PH值调节至7,在高温除去细胞破壁酶;再进行冷冻干燥,然后在0℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为800目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、球藻粉、糖加入到粗发酵液中,在50℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在15℃,得到畜禽补气血提高免疫力的中药酵素;
6)将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
将上述畜禽补气血提高免疫力的中药酵素加入肉猪饲料,加入量为饲料重量的1%,混合均匀,配好后与未加发酵饲料的肉猪饲料进行对照试验。试验猪选择三江白猪60头,选择猪体生长发育正常,健康无病,随机分为试验组2和对照组,两组的品种相同,体重基本一致。
试验在河北某养殖基地进行,试验期30d。现场试验严格按照猪场常规养殖管理进行,分别在07:00、10:00、13:30和15:30对各组仔猪进行拌料饲喂。
在第1天和第30天,对猪进行空腹称重,计算平均日增重。每天记录各组采食量,试验结束,结算各组耗料量,计算平均日采食量和料重比,每天08:00观察猪粪便情况,记录每组仔猪日腹泻头数,计算腹泻率:腹泻率(%)=100×腹泻头数/(试验猪头数×试验天数),结果见表2。
表2试验结果
由上表可知,采用本发明中药酵素的长白种猪平均日增重达573g,比对照组的日增重515g提高了11.26%;料肉比从2.14提高到了1.88,饲料转化率提高了13.83%,腹泻率更低。
实施例3
中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜24份、大枣35份、枸杞12份、熟地黄12份、当归8份、白芍8份、川芎5份、人参8份、白术8份、茯苓8份、炙甘草5份、黄芪12份、肉桂2份、益母草5份、半夏5份、骨碎补8份、决明子5份、百合2.5份、金银花5份、螺旋藻粉3.5份、糖35份、300份山楂、300份草果、黄瓜1400份、苹果2400份、胡萝卜2400份、青果130份。
复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌,双歧杆菌的活菌数为6×108CFU/ml,醋酸杆菌的活菌数为7×107CFU/ml,植物乳杆菌的活菌数为1.4×109CFU/ml,枯草芽孢杆菌的活菌数为3.5×106CFU/ml,米曲霉的活菌数为3.5×105CFU/ml,乳酸菌的活菌数6×108CFU/ml。
畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2.5h,冷却至20℃,得到混合液,备用;其中,水的加入量是生姜、打造、枸杞总质量的10倍;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在32mg/L,发酵温度18℃,搅拌速度12r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为15目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于48℃下酶解10-30min;再超声处理12min;再加入碱性物质,将溶液的PH值调节至6,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-8℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为600~800目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、螺旋藻粉、糖加入到粗发酵液中,在48℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在13℃,得到畜禽补气血提高免疫力的中药酵素;
6)将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
将上述畜禽补气血提高免疫力的中药酵素加入肉猪饲料,加入量为饲料重量的1%,混合均匀,配好后与未加发酵饲料的肉猪饲料进行对照试验。试验猪选择苏联大白猪60头,选择猪体生长发育正常,健康无病,,随机分为试验组3和对照组,两组的品种相同,体重基本一致。
试验在河北某养殖基地进行,试验期30d。现场试验严格按照猪场常规养殖管理进行,分别在07:00、10:00、13:30和15:30对各组仔猪进行拌料饲喂。
在第1天和第30天,对猪进行空腹称重,计算平均日增重。每天记录各组采食量,试验结束,结算各组耗料量,计算平均日采食量和料重比,每天08:00观察猪粪便情况,记录每组猪日腹泻头数,计算腹泻率:腹泻率(%)=100×腹泻头数/(试验猪头数×试验天数),结果见表3。
表3试验结果
由上表可知,采用本发明中药酵素的长白种猪平均日增重达594g,比对照组的日增重531g提高了11.86%;料肉比从2.39提高到了2.21,饲料转化率提高了8.14%,腹泻率更低。
实施例4
中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜27份、大枣45份、枸杞13份、熟地黄13份、当归12份、白芍12份、川芎7份、人参12份、白术12份、茯苓12份、炙甘草7份、黄芪13份、肉桂5份、益母草6份、半夏6份、骨碎补12份、决明子6份、百合3份、金银花6份、球藻粉4.5份、糖45份、400份山楂、400份草果、黄瓜1700份、苹果2600份、胡萝卜2600份、青果160份。
复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌,双歧杆菌的活菌数为7×108CFU/ml,醋酸杆菌的活菌数为9×107CFU/ml,植物乳杆菌的活菌数为1.7×109CFU/ml,枯草芽孢杆菌的活菌数为4.5×106CFU/ml,米曲霉的活菌数为4.5×105CFU/ml,乳酸菌的活菌数为8×108CFU/ml。
畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2.3h,冷却至20℃,得到混合液,备用;其中,水的加入量是生姜、打造、枸杞总质量的10倍;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在35mg/L,发酵温度20℃,搅拌速度12r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为25目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于52℃下酶解25min;再超声处理13min;再加入碱性物质,将溶液的PH值调节至7,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-8℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为750目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、球藻粉、糖加入到粗发酵液中,在43℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在13℃,得到畜禽补气血提高免疫力的中药酵素;
6)将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
将上述畜禽补气血提高免疫力的中药酵素加入肉猪饲料,加入量为饲料重量的1%,混合均匀,配好后与未加发酵饲料的肉猪饲料进行对照试验。试验猪选择汉普夏猪60头,5选择猪体生长发育正常,健康无病5,随机分为试验组4和对照组,两组的品种相同,出生日期和体重基本一致。
试验在河北某养殖基地进行,试验期30d。现场试验严格按照猪场常规养殖管理进行,分别在07:00、10:00、13:30和15:30对各组仔猪进行拌料饲喂。
在第1天和第30天,对猪进行空腹称重,计算平均日增重。每天记录各组采食量,试验结束,结算各组耗料量,计算平均日采食量和料重比,每天08:00观察猪粪便情况,记录每组仔猪日腹泻头数,计算腹泻率:腹泻率(%)=100×腹泻头数/(试验猪头数×试验天数),结果见表4。
表4试验结果
由上表可知,采用本发明中药酵素的长白种猪平均日增重达666g,比对照组的日增重612g提高了8.82%;料肉比从2.04提高到了1.88,饲料转化率提高了8.51%。
实施例5
中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜25份、大枣40份、枸杞12份、熟地黄13份、当归10份、白芍10份、川芎6份、人参10份、白术10份、茯苓10份、炙甘草6份、黄芪12份、肉桂3份、益母草5.5份、半夏5.5份、骨碎补10份、决明子5.5份、百合2.5份、金银花5.5份、螺旋藻粉4份、糖40份、350份山楂、350份草果、黄瓜1500份、苹果2500份、胡萝卜2500份、青果140份。
复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌,双歧杆菌的活菌数为6.5×108CFU/ml,醋酸杆菌的活菌数为8×107CFU/ml,植物乳杆菌的活菌数为1.5×109CFU/ml,枯草芽孢杆菌的活菌数为4×106CFU/ml,米曲霉的活菌数为4×105CFU/ml,乳酸菌的活菌数为7×108CFU/ml。
畜禽补气血提高免疫力的中药酵素的制备方法,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2.7h,冷却至23℃,得到混合液,备用;其中,水的加入量是生姜、打造、枸杞总质量的10倍;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在30mg/L,发酵温度19℃,搅拌速度10r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为20目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于50℃下酶解20min;再超声处理12min;再加入碱性物质,将溶液的PH值调节至6.5,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-5℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为700目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、螺旋藻粉、糖加入到粗发酵液中,在45℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在12℃,得到畜禽补气血提高免疫力的中药酵素;
6)将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
将上述畜禽补气血提高免疫力的中药酵素加入肉猪饲料,加入量为饲料重量的1%,混合均匀,配好后与未加发酵饲料的肉猪饲料进行对照试验。试验猪选择北京黑猪180头,选择猪体生长发育正常,健康无病,随机分为试验组5和对照组,两组的品种相同,体重基本一致。
试验在河北某养殖基地进行,试验期30d。现场试验严格按照猪场常规养殖管理进行,分别在07:00、10:00、13:30和15:30对各组仔猪进行拌料饲喂。
在第1天和第30天,对猪进行空腹称重,计算平均日增重。每天记录各组采食量,试验结束,结算各组耗料量,计算平均日采食量和料重比,结果见表5。
表5试验结果
由上表5可知,采用本发明中药酵素的长白种猪平均日增重达689kg,比对照组的日增重634g提高了8.68%;料肉比从2.65提高到了2.44,饲料转化率提高了8.61%。
综上所述,以上实施例仅用以说明本发明的技术方案而非限制,尽管通过参照本发明的某些优选实施例已经对本发明进行了描述,但本领域的普通技术人员应当理解,可以在形式上和细节上对其做出各种各样的改变,而不偏离所附权利要求书所限定的本发明精神和范围。
Claims (6)
1.一种畜禽补气血提高免疫力的中药酵素,其特征在于,中药酵素由复合菌发酵而成,中药包括以下重量份数的原料:
生姜20-30份、大枣30-50份、枸杞10-15份、熟地黄10-15份、当归5-15份、白芍5-15份、川芎4-8份、人参5-15份、白术5-15份、茯苓5-15份、炙甘草3-8份、黄芪10-15份、肉桂1-5份、益母草3-8份、半夏3-8份、骨碎补5-15份、决明子3-8份、百合2-3份、金银花3-8份、微藻粉3-5份、糖30-50份、200-500份山楂、200-500份草果、黄瓜1000-2000份、苹果2000-3000份、胡萝卜2000-3000份、青果100-200份。
2.根据权利要求1所述的中药酵素,其特征在于,复合菌包括双歧杆菌、醋酸杆菌、植物乳杆菌、枯草芽孢杆菌、米曲霉和乳酸菌。
3.根据权利要求2所述的中药酵素,其特征在于,双歧杆菌的活菌数为5×108-8×108CFU/ml,醋酸杆菌的活菌数为6×107-10×107CFU/ml,植物乳杆菌的活菌数为1×109-2×109CFU/ml,枯草芽孢杆菌的活菌数为3×106-5×106CFU/ml,米曲霉的活菌数为3×105-5×105CFU/ml,乳酸菌的活菌数为4×108-10×108CFU/ml。
4.根据权利要求1所述的中药酵素,其特征在于,微藻粉为螺旋藻粉或小球藻粉。
5.一种根据权利要求1-4任一项所述的畜禽补气血提高免疫力的中药酵素的制备方法,其特征在于,包括以下步骤:
1)将生姜、大枣、枸杞加入到水中煮2-3h,冷却至20~25℃,得到混合液,备用;
2)将山楂、草果、黄瓜、苹果、胡萝卜、青果进行清洗,并将清洗后的山楂、草果、黄瓜、苹果、胡萝卜、青果一起进行破碎处理,打浆,得到浆液;向浆液中加入复合菌,溶氧量控制在25~35mg/L,发酵温度18~20℃,搅拌速度5~15r/min,糖度降至10g/L时,停止发酵,静置72h,得到粗发酵液,备用;
3)将熟地黄、当归、白芍、川芎、人参、白术、茯苓、炙甘草、黄芪、肉桂、益母草、半夏、骨碎补、决明子、百合、金银花粉碎至粒度为10~30目的颗粒;加入柠檬酸溶液和细胞破壁酶,搅拌均匀,于45-55℃下酶解10-30min;再超声处理10~15min;再加入碱性物质,将溶液的PH值调节至6~7,在高温除去细胞破壁酶;再进行冷冻干燥,然后在-10℃-0℃的温度下放置直至溶液的温度升至室温,粉碎至粒度为600~800目的颗粒,得到破壁粉,备用;
4)将步骤1)得到的混合液、步骤2)得到的微破壁粉、微藻粉、糖加入到粗发酵液中,在40-50℃发酵,糖度低于0.5g/L,总酸大于6g/L后终止发酵,迅速降温至15℃,静置72h,得到二次发酵液;
5)将步骤4)得到的二次发酵液用硅藻土过滤机和纸板过滤机进行低温过滤,过滤温度保持在10~15℃,得到畜禽补气血提高免疫力的中药酵素。
6.根据权利要求5所述的制备方法,其特征在于,还包括将步骤5)得到的过滤后的发酵液采用紫外线灯进行杀菌消毒,而后采用装罐设备进行装罐,从而得到畜禽补气血提高免疫力的中药酵素。
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