CN112147770A - Digital hyperspectral imaging microscopic image system and scanning method thereof - Google Patents
Digital hyperspectral imaging microscopic image system and scanning method thereof Download PDFInfo
- Publication number
- CN112147770A CN112147770A CN202011060610.2A CN202011060610A CN112147770A CN 112147770 A CN112147770 A CN 112147770A CN 202011060610 A CN202011060610 A CN 202011060610A CN 112147770 A CN112147770 A CN 112147770A
- Authority
- CN
- China
- Prior art keywords
- digital
- hyperspectral imaging
- display
- camera
- scanning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
Landscapes
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention belongs to the technical field of scientific instruments and equipment, and provides a digital hyperspectral imaging microscopic image system and a scanning method thereof, which mainly comprise an electric biological microscope optical system, a full-automatic digital scanning system, a hyperspectral imaging scanning system and a display system; the device comprises a biological microscope, wherein an electric loading platform is arranged on the biological microscope, and at least one video interface is arranged on the biological microscope; the system comprises a digital scanning camera, a video interface and a video processing module, wherein the digital scanning camera is communicated with the video interface; the imaging camera is in visual communication with the visual interface; the display system comprises a data processing host and a display, wherein the data processing host is electrically connected with the display, the digital scanning camera and the imaging camera. The invention can simultaneously realize various operations on the observed object, effectively reduce the cost for storing the observed object and meet the timeliness of observation and analysis operations.
Description
Technical Field
The invention belongs to the technical field of scientific instruments and equipment, and particularly relates to a digital hyperspectral imaging microscopic image system and a scanning method thereof.
Background
The hyperspectral imaging is a comprehensive technology integrating a detector technology, a precise optical machine, weak signal detection, a computer technology and an information processing technology. The method comprises the steps of detecting two-dimensional geometric space and one-dimensional spectral information of a target simultaneously by a multi-dimensional information acquisition technology combining an imaging technology and a spectrum technology, and acquiring continuous and narrow-band image data with high spectral resolution. The spectral resolution of the hyperspectral image data is up to 10-2 lambda orders of magnitude, the spectral resolution is nano (nm) in the visible to short wave infrared band range, the number of spectral bands is dozens or even hundreds, the spectral bands are usually continuous, and each pixel of the image data can extract a complete high-resolution spectral curve. The system has become one of the high points of scientific and technological competition in developed countries in civil and military, can be used for detecting and identifying cosmic substances, and can effectively and accurately analyze and judge the substances of resources such as geology, mineral deposits, forests, water conservancy, oceans, agriculture and the like by using aerial remote sensing or satellite remote sensing technology; natural disaster prediction, forecast and environmental pollution detection can be carried out in the aspect of weather; cell, chromosome classification, analysis, identification and medical diagnosis can be carried out in the biomedical field; the method is used for military target reconnaissance, guidance, warning system, defense system and anti-camouflage reconnaissance in national security departments such as military, public security and the like, and can be widely applied in industry and have great data value.
The full-automatic digital scanning system can automatically scan and analyze one or more samples simultaneously, not only can save time and labor, but also can obtain clear and stable pictures, and can permanently reserve the samples in a digital form.
However, the hyperspectral imaging system and the full-automatic digital scanning system in the prior art are mutually independent devices, and corresponding operations are performed step by step, so that the observed object needs to be adjusted and sealed for many times, higher requirements are provided for the quality of a slide carrier of the observed object, and the like, scanning and spectral imaging operations of the same observed target in the same time, space and state cannot be performed, and great restrictions are caused to the analysis processes of scientific research, pathology and the like.
Disclosure of Invention
In order to overcome the above disadvantages of the prior art, an object of the present invention is to provide a digital hyperspectral imaging microscopic image system and a scanning method thereof, which can simultaneously implement multiple operations on an observed object, effectively reduce the cost of saving the observed object, and meet the timeliness of the observation and analysis operations.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a digital hyperspectral imaging microscopy image system comprising:
the electric biological microscope optical system comprises a biological microscope, wherein an electric loading platform is arranged on the biological microscope, and at least one video interface is arranged on the biological microscope;
the full-automatic digital scanning system comprises a digital scanning camera, and the digital scanning camera is communicated with the video interface video;
the hyperspectral imaging scanning system comprises an imaging camera, and the imaging camera is communicated with the visual interface visual image;
the display system comprises a data processing host and a display, wherein the data processing host is electrically connected with the display, the digital scanning camera and the imaging camera.
Preferably, the display is a high-definition display or an ultra-high-definition display, and the display is a 2D display or a 3D display.
Preferably, the objective lens vision multiple of the fully automatic digital scanning system is more than 20 times.
Preferably, the pixels of the digital scanning camera are equal to or greater than 230 ten thousand.
Preferably, the fully automatic digital scanning system further comprises a preview camera, and the pixels of the preview camera are greater than or equal to 200 ten thousand.
Preferably, the biological microscope is provided with at least two video interfaces, and the digital scanning camera and the imaging camera are respectively communicated with the video interfaces in a video mode.
Preferably, the objective magnification of the biological microscope includes 10X, 20X, 40X, and 100X, and the biological microscope is provided with a halogen light source.
Preferably, the electric stage has a stroke of 80mm × 60mm, 75mm × 240mm, or 110mm × 120mm, a resolution of less than 0.05um, a maximum speed of 100mm/s, a unidirectional repetition accuracy of 0.1 μm or less, and a bidirectional repetition accuracy of 1 μm or less.
Preferably, the spectral range of the hyperspectral imaging scanning system comprises 400-950nm, 900-1700nm or 1000-2500 nm.
A digital hyperspectral imaging microscopic image scanning method is suitable for the digital hyperspectral imaging microscopic image system and comprises the following steps:
placing the observed object on an electric loading platform;
adopting a full-automatic digital scanning system to carry out integral scanning on the observed object so as to obtain the full appearance of the observed object, and transmitting the obtained information to a data processing host through a video interface for analysis, editing and storage;
the hyperspectral imaging scanning system is replaced through the video interface to scan the observed object, the objective lenses with different multiples are converted after focusing to perform big data detection on the observed object, and the obtained information is also transmitted to the data processing host computer to be analyzed, edited and stored;
the scanning operation of the observed object is shown through the display.
Compared with the prior art, the invention has the beneficial effects that:
in the scheme, at least one video interface is reserved on the biological microscope, the connection with external equipment can be realized through the video interface, such as a full-automatic digital scanning system and a hyperspectral imaging scanning system, thereby the sharing and the multifunctional collinear operation of video information can be realized, the smear or slice scanning and the hyperspectral imaging scanning can be simultaneously carried out in the microscopic observation of the observed object, the transfer of the observed object and the preparation work required to be completed due to the transfer are not needed, the observation synchronism of the observed object and the correspondence of the obtained information are greatly improved, the scientific research cost is reduced, the obtained information can realize the double-digitization processing of specimen digital storage and hyperspectral big data digital analysis, and a new step is developed for scientific research.
Meanwhile, a display system is further arranged, so that the operation of the observed object can be displayed in a display through a video interface, the interesting and taste feeling in scientific research and observation work is greatly improved, the visual angle obtained by an operator is not limited to be obtained from the eyepiece of a single microscope, more students can participate in the operation, the observation angle and the information which is wanted to be obtained can be adjusted in real time, the discovery and the description of details are enhanced, edutainment is really achieved, the attractive feeling and the working efficiency of the scientific research and observation operation are greatly improved, and a powerful tool is provided for basic science and application scientific research.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural diagram of an optical system of an electric biomicroscope according to the present invention.
FIG. 3 is a schematic structural diagram of the present invention using a four-mesh biomicroscope.
FIG. 4 is a schematic view of the optical system (four-eye) of the electric biomicroscope of the present invention.
Wherein:
1-electric biological microscope optical system, 11-biological microscope, 111-video interface, 12-electric loading platform, 2-display system, 21-data processing host, 22-display, 31-digital scanning camera and 41-imaging camera.
Detailed Description
So that the manner in which the above recited objects, features and advantages of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may be had by reference to the embodiments thereof which are illustrated in the appended drawings. In addition, the embodiments and features of the embodiments of the present application may be combined with each other without conflict. In the following description, numerous specific details are set forth to provide a thorough understanding of the present invention, and the described embodiments are merely a subset of the embodiments of the present invention, rather than a complete embodiment. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Example 1
As shown in fig. 1 to 4, the present embodiment provides a digital hyperspectral imaging microscopic image system, which mainly includes an optical system 1 of an electric biological microscope, a full-automatic digital scanning system, a hyperspectral imaging scanning system, and a display system 2; other structural forms of the electric biomicroscope optical system 1, the full-automatic digital scanning system, the hyperspectral imaging scanning system and the display system 2 in the embodiment can refer to the prior art, and are not described herein again.
Specifically, the electric biomicroscope optical system 1 comprises a biomicroscope 11, wherein an electric loading platform 12 is arranged on the biomicroscope 11, and at least one video interface 111 is arranged on the biomicroscope 11; the full-automatic digital scanning system comprises a digital scanning camera 31, wherein the digital scanning camera 31 is in visual communication with the visual interface 111; the hyperspectral imaging scanning system comprises an imaging camera 41, wherein the imaging camera 41 is in visual communication with the visual interface 111; the method and the device have the advantages that the simultaneous observation and analysis of the same observed object are realized, the problem that in the prior art, when the observed object needs to be processed by a full-automatic digital scanning system and then moves to a hyperspectral imaging scanning system, smears or slices need to be stored, moved and readjusted is avoided, the timeliness and the correspondence of observed information are effectively guaranteed, and the accuracy of scientific research and observation is improved.
The display system 2 comprises a data processing host 21 and a display 22, wherein the data processing host 21 is electrically connected with the display 22, the digital scanning camera 31 and the imaging camera 41, so that the scanning operation of the observed object can be observed under the display 22 in the whole process, and information sharing is realized.
As a preferable scheme, the display 22 is a high definition display or an ultra high definition display, and the display 22 can be selectively set as a 2D display or a 3D display; when the 3D display is adopted, naked-eye type 3D display can be adopted, and glasses-wearing type 3D display can also be adopted.
In addition, the objective lens vision multiple of the fully automatic digital scanning system is more than 20 times, preferably 20 to 40 times in the embodiment, and the pixel of the digital scanning camera 31 is more than or equal to 230 ten thousand. Further, the fully automatic digital scanning system further comprises a preview camera (not shown in the figure), and the pixel of the preview camera is greater than or equal to 200 ten thousand.
As a preferable scheme, the biological microscope 11 in this embodiment can be selectively configured as a trinocular biological microscope, at this time, the biological microscope 11 is provided with one of the video interfaces 111, and real-time observation of video information can be realized through the switching connection between the digital scanning camera 31 and the imaging camera 41; as a more preferable scheme, the biological microscope 11 may be preferably configured as a four-eye biological microscope, and in this case, two of the image interfaces 111 are provided, so that the digital scanning camera 31 and the imaging camera 41 may be respectively and visually connected to the image interfaces 111 to achieve synchronous observation and avoid switching operation. Further, the objective magnification of the biological microscope 11 includes 10X, 20X, 40X, and 100X, and the biological microscope 11 is provided with a halogen light source.
In addition, the stroke of the electric carrying platform 12 is more than or equal to 80mm × 60mm or 75mm × 240mm or 110mm × 120mm, the resolution is less than 0.05um, the maximum speed is 100mm/s, the unidirectional repetition precision is less than or equal to 0.1 μm, and the bidirectional repetition precision is less than or equal to 1 μm. Further, the observation area of the electric stage 12 can simultaneously satisfy that 12 observed objects are scanned simultaneously.
As a preferable scheme, the spectral range of the hyperspectral imaging scanning system comprises 400-.
In order to facilitate further understanding of the present disclosure, a digital hyperspectral imaging microscopic image scanning method is further provided in this embodiment, and is applicable to the above digital hyperspectral imaging microscopic image system, and specifically includes the following steps:
s1, placing the observed object such as a smear or a slice on the electric loading platform 12, and adjusting the position state of the electric loading platform 12 to adapt to the instrument equipment to be operated.
S2, the full-automatic digital scanning system is used to scan the observed object to obtain the overall image of the observed object, and the full-automatic digital scanning system can share the image information of the optical system 1 of the electric biomicroscope in real time through the image interface 111, and then transmit the obtained information to the data processing host 21 for analysis, editing and storage, so as to realize the digitization of the specimen.
And S3, the hyperspectral imaging scanning system is replaced through the video interface 111 to scan the observed object, the objective lenses with different multiples are converted after focusing to detect the big data of the observed object, and the obtained information is also transmitted to the data processing host 21 to be analyzed, edited and stored, so that the digitization of the hyperspectral scanning big data is realized.
S4, the scanning operation of the observed object is shown through the display 22.
Further, the order of the above steps S2 and S3 may be exchanged or synchronized.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (10)
1. A digital hyperspectral imaging microscopic image system is characterized by comprising:
the electric biological microscope optical system comprises a biological microscope, wherein an electric loading platform is arranged on the biological microscope, and at least one video interface is arranged on the biological microscope;
the full-automatic digital scanning system comprises a digital scanning camera, and the digital scanning camera is communicated with the video interface video;
the hyperspectral imaging scanning system comprises an imaging camera, and the imaging camera is communicated with the visual interface visual image;
the display system comprises a data processing host and a display, wherein the data processing host is electrically connected with the display, the digital scanning camera and the imaging camera.
2. The digital hyperspectral imaging microscopy image system according to claim 1, wherein the display is a high-definition display or an ultra-high-definition display, and the display is a 2D display or a 3D display.
3. The digital hyperspectral imaging microscopy image system according to claim 1, wherein the objective optic magnification of the fully automatic digital scanning system is greater than 20 times.
4. The digital hyperspectral imaging microscopy image system according to claim 3, wherein the digital scanning camera has a pixel equal to or greater than 230 ten thousand.
5. The digital hyperspectral imaging microscopy image system according to claim 4, wherein the fully automatic digital scanning system further comprises a preview camera, wherein the pixel of the preview camera is greater than or equal to 200 ten thousand.
6. The digital hyperspectral imaging microscopic image system according to claim 1, wherein at least two of the image interfaces are arranged on the biological microscope, and the digital scanning camera and the imaging camera are respectively arranged on the image interfaces in a visual communication manner.
7. The digital hyperspectral imaging microscopy image system according to any of claims 1 to 6, wherein the objective magnification of the biomicroscope comprises 10X, 20X, 40X, 100X, and the biomicroscope is equipped with a halogen light source.
8. The digital hyperspectral imaging microscopy image system according to claim 7, wherein the electric stage has a stroke of 80mm by 60mm or 75mm by 240mm or 110mm by 120mm, a resolution of less than 0.05um, a maximum speed of 100mm/s, a unidirectional repetition precision of 0.1 μm or less, and a bidirectional repetition precision of 1 μm or less.
9. The digital hyperspectral imaging microscopy image system according to claim 1 or 8, wherein the spectral range of the hyperspectral imaging scanning system comprises 400-950nm, 900-1700nm or 1000-2500 nm.
10. A digital hyperspectral imaging microscopic image scanning method, which is suitable for the digital hyperspectral imaging microscopic image system of any one of claims 1 to 9, and is characterized by comprising the following steps:
placing the observed object on an electric loading platform;
adopting a full-automatic digital scanning system to carry out integral scanning on the observed object so as to obtain the full appearance of the observed object, and transmitting the obtained information to a data processing host through a video interface for analysis, editing and storage;
the hyperspectral imaging scanning system is replaced through the video interface to scan the observed object, the objective lenses with different multiples are converted after focusing to perform big data detection on the observed object, and the obtained information is also transmitted to the data processing host computer to be analyzed, edited and stored;
the scanning operation of the observed object is shown through the display.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011060610.2A CN112147770A (en) | 2020-09-30 | 2020-09-30 | Digital hyperspectral imaging microscopic image system and scanning method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011060610.2A CN112147770A (en) | 2020-09-30 | 2020-09-30 | Digital hyperspectral imaging microscopic image system and scanning method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112147770A true CN112147770A (en) | 2020-12-29 |
Family
ID=73951297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011060610.2A Pending CN112147770A (en) | 2020-09-30 | 2020-09-30 | Digital hyperspectral imaging microscopic image system and scanning method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112147770A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1057910A (en) * | 1990-07-04 | 1992-01-15 | 中国科学院长春光学精密机械研究所 | Scanning multispectral microscopic imaging method and device thereof |
| CN102854615A (en) * | 2012-04-27 | 2013-01-02 | 麦克奥迪实业集团有限公司 | Full-automatic scanning system and method for microscopic section |
| CN103411934A (en) * | 2013-07-18 | 2013-11-27 | 江苏大学 | Fluorescence microscopy hyperspectrum imaging system |
| CN109342328A (en) * | 2018-10-11 | 2019-02-15 | 中国科学院西安光学精密机械研究所 | A kind of built-in scan type high microspectrum imaging system and imaging method |
-
2020
- 2020-09-30 CN CN202011060610.2A patent/CN112147770A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1057910A (en) * | 1990-07-04 | 1992-01-15 | 中国科学院长春光学精密机械研究所 | Scanning multispectral microscopic imaging method and device thereof |
| CN102854615A (en) * | 2012-04-27 | 2013-01-02 | 麦克奥迪实业集团有限公司 | Full-automatic scanning system and method for microscopic section |
| CN103411934A (en) * | 2013-07-18 | 2013-11-27 | 江苏大学 | Fluorescence microscopy hyperspectrum imaging system |
| CN109342328A (en) * | 2018-10-11 | 2019-02-15 | 中国科学院西安光学精密机械研究所 | A kind of built-in scan type high microspectrum imaging system and imaging method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8350905B2 (en) | Microscope system, image generating method, and program for practicing the same | |
| JP6437947B2 (en) | Fully automatic rapid microscope slide scanner | |
| JP6915349B2 (en) | Image processing equipment, image processing method, and image processing program | |
| Gao et al. | Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy | |
| Patel et al. | Contemporary whole slide imaging devices and their applications within the modern pathology department: a selected hardware review | |
| EP2941663B1 (en) | A method for automated platform and/or reference object independent acquisition of positional information and localization of objects of interest in a microscope | |
| CN106872034B (en) | Quick three-dimensional muti-spectrum imaging system | |
| CN109541791A (en) | High-resolution light field micro imaging system and method based on sub-pix translation | |
| CN104568873B (en) | A kind of laser scanning co-focusing microscope being imaged to fluorescent material | |
| US11347044B2 (en) | Low resolution slide imaging and slide label imaging and high resolution slide imaging using dual optical paths and a single imaging sensor | |
| CN102279048B (en) | Broadband microscopic imaging spectrum system and wavelength conversion rapid focusing control method thereof | |
| EP3803495A1 (en) | Analyzer for three-dimensionally analyzing a medical sample by means of a light-field camera | |
| CN102788756A (en) | Multi-modal biological microscope analyzer | |
| EP4063836A1 (en) | Algae analysis method | |
| CN103926000A (en) | Image spectrum detection device | |
| CN105372803A (en) | Depth of field-increasing microscopic optical system | |
| EP3547674B1 (en) | Image processing device, microscope system, image processing method, and program | |
| CN101539456B (en) | General hyperspectral imaging component | |
| CN213149364U (en) | Digital hyperspectral imaging microscopic image system | |
| CN213749626U (en) | Hyperspectral imaging microscopic big data measurement system | |
| CN112147770A (en) | Digital hyperspectral imaging microscopic image system and scanning method thereof | |
| Aidukas et al. | High-speed multi-objective Fourier ptychographic microscopy | |
| WO2021105801A1 (en) | Method for digitally staining cells | |
| CN1170183C (en) | High-speed laser-confocal scanning microscopic imaging apparatus | |
| CN112213310A (en) | Hyperspectral imaging microscopic big data measurement system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |