CN112213310A - Hyperspectral imaging microscopic big data measurement system and method - Google Patents
Hyperspectral imaging microscopic big data measurement system and method Download PDFInfo
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- CN112213310A CN112213310A CN202011210737.8A CN202011210737A CN112213310A CN 112213310 A CN112213310 A CN 112213310A CN 202011210737 A CN202011210737 A CN 202011210737A CN 112213310 A CN112213310 A CN 112213310A
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Abstract
The invention discloses a hyperspectral imaging microscopic big data measurement system which comprises a microscope assembly, wherein the microscope assembly comprises a microscope module, a measurement microscope module and a hyperspectral imaging module, the microscope module is provided with a measurement eyepiece and a hyperspectral eyepiece, the measurement microscope module is connected with the measurement eyepiece, and the hyperspectral imaging module is connected with the hyperspectral eyepiece; the hyperspectral imager also comprises a data processing module, wherein the data processing module is electrically connected with the microscope component so as to perform data processing on data information obtained by measurement and analysis of the measurement microscope module and a hyperspectral image obtained by processing of the hyperspectral imaging module and establish a hyperspectral image measurement big database; the display is respectively and electrically connected with the microscope component and the data processing module so as to display the image of the specimen and the measurement and analysis information acquired by the microscope component and the image and the information processed by the data processing module. The invention also discloses a measurement method based on the hyperspectral imaging microscopic big data measurement system.
Description
Technical Field
The invention relates to the technical field of imaging measuring instruments, in particular to a hyperspectral imaging microscopic big data measuring system and a hyperspectral imaging microscopic big data measuring method.
Background
With the development of science and technology, the requirements for various image recognition technologies are also metAre increasingly high, particularly for spectral imaging techniques that are increasingly widely used. The spectral imaging technology can play a better role in the field of observing cell activities, in particular to the hyperspectral imaging technology, and the hyperspectral imaging technology is adopted to reach 10-2And the resolution of lambda order of magnitude, and each pixel of the obtained image data can extract a complete high-resolution spectral curve. However, in the prior art, the image recognition device is used alone for pattern recognition, the measured data is single, repeated measurement can be caused, the measurement efficiency is low, the error is large, and the single measurement means can not meet the requirement of modern automation intelligence.
Disclosure of Invention
The invention aims to provide a hyperspectral imaging microscopic big data measurement system and a hyperspectral imaging microscopic big data measurement method, which can realize automatic and intelligent hyperspectral imaging microscopic big data measurement by combining a hyperspectral imaging technology and big data measurement, and have the advantages of simple operation, higher measurement efficiency and more accurate measured data.
In order to solve the problems, the invention is realized according to the following technical scheme:
the invention provides a hyperspectral imaging microscopic big data measurement system, which comprises:
the microscope assembly comprises a microscope module, a measurement microscope module and a hyperspectral imaging module, wherein the microscope module is used for acquiring image information of a specimen, a measurement eyepiece and a hyperspectral eyepiece are arranged on the microscope module, the measurement microscope module is connected with the measurement eyepiece, the hyperspectral imaging module is connected with the hyperspectral eyepiece, the measurement microscope module is used for measuring and analyzing an image of the specimen, and the hyperspectral imaging module is used for receiving and processing the image of the specimen to form a hyperspectral image;
the data processing module is electrically connected with the microscope component so as to perform data processing on data information obtained by measurement and analysis of the measurement microscope module and a hyperspectral image obtained by processing of the hyperspectral imaging module and establish a hyperspectral image measurement big database;
and the display is respectively and electrically connected with the microscope component and the data processing module so as to display the image of the specimen and the measurement and analysis information acquired by the microscope component and the image and the information processed by the data processing module.
Further, the microscope module includes a stereo microscope and a bio microscope.
Further, the stereoscopic microscope is a four-eye stereoscopic microscope, wherein the four-eye stereoscopic microscope comprises a first pair of stereoscopic eyepieces and a second pair of stereoscopic eyepieces, the first pair of stereoscopic eyepieces are arranged as human eye stereoscopic eyepieces, and the second pair of stereoscopic eyepieces comprise a stereoscopic measuring eyepiece and a stereoscopic hyperspectral eyepiece; the four-eye stereomicroscope is internally or externally provided with an adjustable halogen light source.
Further, the stereoscopic microscope further comprises a universal rotating frame, and the stereoscopic microscope can be rotatably arranged on the universal rotating frame by 360 degrees.
Further, the biological microscope is configured as a four-eye biological microscope, wherein the four-eye biological microscope comprises a first pair of biological eyepieces and a second pair of biological eyepieces, the first pair of biological eyepieces is configured as a human eye biological eyepiece, and the second pair of biological eyepieces comprises a biological measurement eyepiece and a biological hyperspectral eyepiece; an adjustable halogen light source is arranged in the four-eye biological microscope.
Further, the measurement microscope module comprises a measurement camera, and the resolution of the measurement camera is greater than or equal to 4K.
Further, the hyperspectral imaging module comprises a hyperspectral imager, the hyperspectral imager comprises a staring type or push-broom type hyperspectral, and the spectral range is 400-; the hyperspectral imager is characterized by further comprising a carrying platform used for being matched with the hyperspectral imager, wherein the carrying platform is set to be a manual carrying platform or an electric carrying platform.
Further, the display is set to be higher than or equal to 24 inches of ultra-high definition display, the display mode is set to be 2D display or 3D display, and the 3D display can be naked eye 3D display or glasses-worn 3D display.
A hyperspectral imaging microscopic big data measurement method is characterized by comprising the hyperspectral imaging microscopic big data measurement system and further comprising the following steps:
s1, placing the specimen on the microscope module, and then operating the microscope module by a measuring person to obtain an image of the specimen;
s2, the image information obtained by the microscope module is measured and analyzed by the measuring microscope module;
s3, performing image processing on the image information acquired by the microscope module through the hyperspectral imaging module to form a hyperspectral image; step S2 and step S3 may be performed simultaneously, or step S3 may be performed first and the operation of step S2 may be performed.
And S4, displaying the data information processed by the microscope component and the data processing module through the display.
Further, in step S1, the hyperspectral imaging microscopy big data measurement system according to claim 2 is further included, wherein during the measurement, the stereomicroscope can rotate 360 ° through the universal rotating rack, so as to measure the full view of the specimen, obtain a full view image of the specimen, and store the full view image into the hyperspectral image big database.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a hyperspectral imaging microscopic big data measurement system and a hyperspectral imaging microscopic big data measurement method. By the system and the method, the measurement is more automatic and intelligent, and the measurement efficiency and the measurement accuracy are improved.
The hyperspectral imaging microscopic big data measurement system and the hyperspectral imaging microscopic big data measurement method provided by the invention are convenient for carrying out the big data measurement of various specimens in the microscopic world, and particularly provide a brand-new big data measurement system and a brand-new big data measurement method for the big data measurement of medical specimens, thereby providing equipment and technical support for original innovation.
Drawings
Embodiments of the invention are described in further detail below with reference to the attached drawing figures, wherein:
FIG. 1 is a schematic structural diagram of a hyperspectral imaging microscopic big data measurement system according to the invention;
FIG. 2 is a schematic view of the stereomicroscope of the present invention;
FIG. 3 is a schematic view of the apparatus connection of the stereomicroscope of the present invention;
FIG. 4 is a schematic structural view of a biological microscope according to the present invention;
FIG. 5 is a schematic view of the apparatus connection of the stereomicroscope of the present invention;
FIG. 6 is a schematic flow chart of a hyperspectral imaging microscopic big data measurement method according to the invention;
in the figure:
100-a microscope component; 110-stereoscopic microscope, 111-first pair of stereoscopic eyepieces; 112-a second pair of stereoscopic eyepieces; 1121-stereoscopic measuring eyepiece; 1122-stereoscopic hyperspectral eyepiece; 113-an optical zoom lens; 120-biomicroscope; 121-a first pair of bio-oculars; 122-a second pair of bio-oculars; 1221-biometric eyepiece; 1222-biological hyperspectral eyepiece; 123-objective lens; 130-a measurement microscopy module; 140-a hyperspectral imaging module; 200-a data processing module; 300-a display; 400-universal swivel mount; 410-a base; 420-a support frame; 430-a universal rotation mechanism; 500-data lines; 600-data processing host.
Detailed Description
In order that the above objects, features and advantages of the present application can be more clearly understood, a detailed description of the present application will be given below with reference to the accompanying drawings and detailed description. In addition, the embodiments and features of the embodiments of the present application may be combined with each other without conflict. In the following description, numerous specific details are set forth to provide a thorough understanding of the present application, and the described embodiments are merely a subset of the embodiments of the present application, rather than all embodiments. All other embodiments obtained by a person of ordinary skill in the art without any inventive work based on the embodiments in the present application are within the scope of protection of the present application.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Further, the description of the upper, lower, left, right, etc. used in the present invention is only with respect to the positional relationship of the respective components of the present invention with respect to each other in the drawings. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
As shown in fig. 1-5, a hyperspectral imaging microscopic big data measurement system comprises a microscope assembly 100, the microscope assembly 100 comprises a microscope module, a measurement microscope module 130 and a hyperspectral imaging module 140, the microscope module is used for acquiring image information of a specimen, the microscope module is provided with a measurement eyepiece and a hyperspectral eyepiece, the measurement microscope module 130 is connected with the measurement eyepiece, the hyperspectral imaging module 140 is connected with the hyperspectral eyepiece, the measurement microscope module 130 is used for measuring and analyzing an image of the specimen, and the hyperspectral imaging module 140 is used for receiving and processing the image of the specimen to form a hyperspectral image.
The hyperspectral image measurement system further comprises a data processing module 200, wherein the data processing module 200 is electrically connected with the microscope assembly 100 so as to perform data processing on data information obtained by measurement and analysis of the measurement microscope module 130 and a hyperspectral image obtained by processing of the hyperspectral imaging module 140, establish a hyperspectral image measurement big database and provide a basis for realizing automatic intelligent big data processing.
The microscope assembly 100 and the data processing module 200 are respectively connected with the microscope assembly 300 and the data processing module 200, so that the images of the specimen and the measurement analysis information acquired by the microscope assembly 100 and the images and the information processed by the data processing module 200 are displayed, a measurement person can visually see the result of the information processing, and the measurement data can be further analyzed and displayed.
Spectral resolution of the hyperspectral image data is up to 10-2The resolution of the spectrum is nanometer in the range of visible to short wave infrared band, the number of the spectrum bands is dozens or even hundreds, the spectrum bands are usually continuous, and each pixel of the image data can extract a complete high resolution spectrum curve. The system has become one of the high points of scientific and technological competition in developed countries in civil and military, can be used for detecting and identifying cosmic substances, and can effectively and accurately analyze and judge the substances of resources such as geology, mineral deposits, forests, water conservancy, oceans, agriculture and the like by using aerial remote sensing or satellite remote sensing technology. In the aspect of weather, natural disaster prediction, forecast and environmental pollution detection can be carried out. Cell, chromosome classification, analysis, identification and medical diagnosis can be performed in the biomedical field. The system is used for military target reconnaissance, guidance, warning system, defense system and anti-camouflage reconnaissance in national security departments such as military affairs and public security. Therefore, the hyperspectral imaging module 140 adopted in the invention can collect and store the various data information in the form of hyperspectral images through the data processing module 200 to form a big database, and the big data can be measured more automatically and intelligently during subsequent measurement.
Specifically, the microscope module includes a stereoscopic microscope 110, in this embodiment, the stereoscopic microscope 110 adopts a four-eye stereoscopic microscope 110, wherein the four-eye stereoscopic microscope 110 includes a first pair of stereoscopic eyepieces 111 and a second pair of stereoscopic eyepieces 112, the first pair of stereoscopic eyepieces 111 are configured as human eye stereoscopic eyepieces, the second pair of stereoscopic eyepieces 112 include stereoscopic measurement eyepieces 1121 and stereoscopic hyperspectral eyepieces 1122, the stereoscopic measurement eyepieces 1121 are connected to the measurement microscope module 130, and the stereoscopic hyperspectral eyepieces 1122 are connected to the hyperspectral imaging module 140. The first pair of stereoscopic eyepieces 111 is inclined forward by 45 degrees, so that an operator can observe conveniently, and the stereoscopic measuring eyepieces 1121 and the stereoscopic hyperspectral eyepieces 1122 of the second pair of stereoscopic eyepieces 112 are respectively inclined to the left and right symmetrically by 30-45 degrees, so that collision is avoided when the measuring microscope module 130 and the hyperspectral imaging module 140 are connected. The four-eye stereomicroscope 110 further includes an optical zoom lens 113, the specification of the optical zoom lens 113 is 0.7X-7X continuous zoom optical lens, which can obtain clear image information, and further includes an internal or external adjustable halogen light source.
Further, the universal rotating frame 400 is further included, and the stereoscopic microscope 110 can be rotatably arranged on the universal rotating frame 400 by 360 degrees, so that the full view of the specimen can be measured directly by rotating the stereoscopic microscope 110, and the full view image of the specimen can be obtained. The universal rotating frame 400 comprises a base 410, a supporting frame 420 is arranged on the base 410, a universal rotating mechanism 430 is arranged on the supporting frame 420, the universal rotating frame 400 is rotatably connected with the stereoscopic microscope 110 through the universal rotating mechanism 430, and the stereoscopic microscope 110 can rotate 360 degrees through the universal rotating mechanism 430.
The microscope assembly 100 further includes a biological microscope 120, in this embodiment, the biological microscope 120 is a four-eye biological microscope 120, wherein the four-eye biological microscope 120 includes a first pair of biological eyepieces 121 and a second pair of biological eyepieces 122, the first pair of biological eyepieces 121 is configured as a human eye biological eyepiece, the second pair of biological eyepieces 122 includes a biological measurement eyepiece 1221 and a biological hyperspectral eyepiece 1222, the biological measurement eyepiece 1221 is connected with the measurement microscope module 130, and the biological hyperspectral eyepiece 1222 is connected with the hyperspectral imaging module 140. The first pair of biological eyepieces 121 are inclined forward by 45 degrees, so that an operator can observe the biological eyepieces conveniently, and the biological measurement eyepieces 1221 and the biological hyperspectral eyepieces 1222 of the second pair of biological eyepieces 122 are respectively inclined towards the left and right symmetry by 15-45 degrees, so that collision is avoided when the measurement microscope module 130 and the hyperspectral imaging module 140 are connected. The biological microscope 120 is further provided with four objective lenses 123 with different multiples, and the magnification specifications of the four objective lenses 123 are respectively 4X, 10X, 40X and 100X so as to meet the measurement conditions of most samples.
The measurement microscope module 130 further includes a measurement camera, the resolution of the measurement camera is greater than or equal to 4K, specifically, the resolution reaches at least 3840 × 2160, the obtained picture is clearer, and the measured data is more accurate. The measurement microscopy module 130 further comprises measurement software, controlled by the measurement software such that the measurement microscopy module 130 includes, but is not limited to, the following functions: photographing, recording, measuring and storing. The measurement microscope module 130 is connected to the display 300 via a data line 500, so that data measured by the stereoscopic microscope 110 or the biological microscope 120 are transmitted to the display 300.
In this embodiment, the hyperspectral imaging module 140 comprises a staring type or push-and-sweep type hyperspectral imager with a spectral range of 400-. The multifunctional microscope is characterized by further comprising a carrying platform, wherein the carrying platforms of the stereoscopic microscope 110 and the biological microscope 120 of the push-broom type hyperspectral imager are arranged to be electric platforms, and the carrying platforms of the stereoscopic microscope 110 and the biological microscope 120 of the staring type hyperspectral imager are manual carrying platforms or electric carrying platforms, so that the carrying platforms can be conveniently adjusted to obtain specimen images meeting requirements. The hyperspectral imaging module 140 is electrically connected with the display through the data processing host 600, the hyperspectral imaging module 140, the data processing host 600 and the display 300 are connected through the data line 500, and the hyperspectral imaging module 140 transmits specimen hyperspectral images obtained through the stereoscopic microscope 110 or the biological microscope 120 to the display 300 for display after being processed by the data processing host 600.
In this embodiment, the adopted display 300 is an ultra high definition display 300 with a size of 24 inches or more, the resolution at least reaches 3840 × 2160, and the display mode may be 2D or 3D, wherein the 3D display may be 3D display with glasses or 3D display with naked eyes, so that the system has a wider applicable range and is more convenient to implement and popularize.
As shown in fig. 1 to 6, the present invention further provides a hyperspectral imaging microscopic big data measurement method, including the hyperspectral imaging microscopic big data measurement system, further including the following steps:
s1, placing the specimen on the microscope module, and then operating the microscope module by a measuring person to obtain an image of the specimen;
s2, performing measurement and analysis on the image information acquired by the microscope module through the measurement microscope module 130;
s3, performing image processing on the image information acquired by the microscope module through the hyperspectral imaging module 140 to form a hyperspectral image; step S2 and step S3 may be performed simultaneously, or step S3 may be performed first and the operation of step S2 may be performed.
And S4, displaying the data information processed by the microscope component and the data processing module through the display.
Specifically, in S1, during the measurement, the stereomicroscope 110 can rotate 360 ° through the gimbaled rotating frame 400, so as to measure the full view of the specimen, obtain a full view image of the specimen, and store the full view image in the hyperspectral image big database. The step S2 and the step S3 may be performed synchronously, or the step S3 may be performed before the step S2 is performed, so that the step of measuring and analyzing is more conveniently adjusted by the measuring staff according to actual needs, the operation of the measuring staff is facilitated, and the measurement efficiency is improved.
The hyperspectral imaging microscopic big data measurement system and the hyperspectral imaging microscopic big data measurement method provided by the invention are convenient for carrying out the big data measurement of various specimens in the microscopic world, and particularly provide a brand-new big data measurement system and a brand-new big data measurement method for the big data measurement of medical specimens, thereby providing equipment and technical support for original innovation.
While the preferred embodiments of the present invention have been illustrated and described in detail, it should be understood that modifications and variations can be effected by one skilled in the art in light of the above teachings without undue experimentation. Therefore, any technical solutions that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the prior art according to the present inventive concept should be within the scope of protection defined by the present claims.
Claims (10)
1. A hyperspectral imaging microscopic big data measurement system is characterized by comprising:
the microscope assembly comprises a microscope module, a measurement microscope module and a hyperspectral imaging module, wherein the microscope module is used for acquiring image information of a specimen, a measurement eyepiece and a hyperspectral eyepiece are arranged on the microscope module, the measurement microscope module is connected with the measurement eyepiece, the hyperspectral imaging module is connected with the hyperspectral eyepiece, the measurement microscope module is used for measuring and analyzing an image of the specimen, and the hyperspectral imaging module is used for receiving and processing the image of the specimen to form a hyperspectral image;
the data processing module is electrically connected with the microscope component so as to perform data processing on data information obtained by measurement and analysis of the measurement microscope module and a hyperspectral image obtained by processing of the hyperspectral imaging module and establish a hyperspectral image measurement big database;
and the display is respectively and electrically connected with the microscope component and the data processing module so as to display the image of the specimen and the measurement and analysis information acquired by the microscope component and the image and the information processed by the data processing module.
2. The hyperspectral imaging microscopy big data measurement system of claim 1, wherein the microscope module comprises a stereo microscope and a bio-microscope.
3. The hyperspectral imaging microscopic big data measurement system according to claim 2, wherein the stereoscopic microscope is configured as a four-eye stereoscopic microscope, wherein the four-eye stereoscopic microscope comprises a first pair of stereoscopic eyepieces and a second pair of stereoscopic eyepieces, the first pair of stereoscopic eyepieces is configured as a human eye stereoscopic eyepiece, and the second pair of stereoscopic eyepieces comprises a stereoscopic measurement eyepiece and a stereoscopic hyperspectral eyepiece; the four-eye stereomicroscope is internally or externally provided with an adjustable halogen light source.
4. The hyperspectral imaging microscopic big data measurement system according to any one of claims 2 or 3, further comprising a universal rotating stand, wherein the stereoscopic microscope can be rotatably arranged on the universal rotating stand by 360 degrees.
5. The hyperspectral imaging microscopy big data measurement system of claim 2, wherein the biomicroscope is configured as a quadrupcould biomicroscope, wherein the quadrupcould biomicroscope comprises a first pair of biomicroscope configured as a human eye biomicroscope and a second pair of biomicroscope comprising a biomeasurement eyepiece and a biological hyperspectral eyepiece; an adjustable halogen light source is arranged in the four-eye biological microscope.
6. The hyperspectral imaging microscopy big data measurement system according to claim 1, wherein the measurement microscopy module comprises a measurement camera, and the resolution of the measurement camera is greater than or equal to 4K.
7. The hyperspectral imaging microscopic big data measurement system according to claim 1, wherein the hyperspectral imaging module comprises a hyperspectral imager, the hyperspectral imager comprises a staring type or push-broom type hyperspectral, and the spectral range is 400-; the hyperspectral imager is characterized by further comprising a carrying platform used for being matched with the hyperspectral imager, wherein the carrying platform is set to be a manual carrying platform or an electric carrying platform.
8. The hyperspectral imaging microscopic big data measurement system according to claim 1, wherein the display is set to be 24 inches or more of ultra-high definition display, the display mode is set to be 2D display or 3D display, and the 3D display can be naked eye 3D display or glasses-worn 3D display.
9. A hyperspectral imaging microscopic big data measurement method, characterized by comprising the hyperspectral imaging microscopic big data measurement system of any one of claims 1 to 8, and further comprising the steps of:
s1, placing the specimen on the microscope module, and then operating the microscope module by a measuring person to obtain an image of the specimen;
s2, the image information obtained by the microscope module is measured and analyzed by the measuring microscope module;
s3, performing image processing on the image information acquired by the microscope module through the hyperspectral imaging module to form a hyperspectral image; step S2 and step S3 may be performed simultaneously, or step S3 may be performed first and the operation of step S2 may be performed.
And S4, displaying the data information processed by the microscope component and the data processing module through the display.
10. The method for measuring the hyperspectral imaging microscopic big data according to claim 7, wherein in step S1, the method further comprises the hyperspectral imaging microscopic big data measurement system according to claim 2, wherein in the measurement process, the stereomicroscope can rotate 360 degrees through the universal rotating frame, so as to measure the full view of the specimen, obtain the full view image of the specimen, and store the full view image in the hyperspectral image big database.
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