CN1170183C - High-speed laser-confocal scanning microscopic imaging apparatus - Google Patents
High-speed laser-confocal scanning microscopic imaging apparatus Download PDFInfo
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- CN1170183C CN1170183C CNB021044538A CN02104453A CN1170183C CN 1170183 C CN1170183 C CN 1170183C CN B021044538 A CNB021044538 A CN B021044538A CN 02104453 A CN02104453 A CN 02104453A CN 1170183 C CN1170183 C CN 1170183C
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Abstract
The present invention relates to a microscopic imaging instrument, particularly to a microscopic imaging instrument applied to medical science. The present invention solves the problem of the prior art that imaging speed is low, etc. The present invention adopts a technical scheme that a system is in a confocal structure, namely that an object to be imaged is positioned on the focal line of an objective lens, a photoelectric sensor is positioned on the focal line of the imaging objective lens, and the objective lens makes one-dimensional scanning along the axial direction or the radical direction; the object to be imaged can also be positioned on the focal point of the objective lens, the photoelectric sensor can also be positioned on the focal point of the imaging objective lens, a cylindrical lens is used as the objective lens, a linear array CCD device is adopted as the photoelectric sensor, and a laser of an infrared band is used as a light source. The present invention has the characteristics of high speed, small volume, simple structure, etc.
Description
Technical field
The present invention relates to a kind of microscopic imaging apparatus, especially relate to the microscopic imaging apparatus of medically using.
Background technology
1987, first laser scanning co-focusing microscope succeeded in developing in the world, and it becomes medical science, the most advanced and strong analytical instrument in cell biology field immediately.Up to now, many scholars are arranged both at home and abroad, proposed various laser scanning confocal micro-scopes at the research laser scanning confocal micro-scope.Adopted one group of replaceable combinations of pairs of forming by exciter filter, double color reflection mirror, inhibition filter as practicality novel (number of patent application 99240337.5), advantage such as make not only that system has kept original light path simplification, compact conformation, volume is little and with low cost, the system that also makes has realized the 3-D view test to various fluorescence and non-fluorescent samples; Patent of invention (application number 98111188.2) discloses a kind of laser cofocus scanning device, be characterized in only sample laser lighting light path, sample scattering light signal light path and observation optical path being integrated as much as possible, thereby make light path simplification, compact conformation, smaller volume, cost lower with a half reflection semi-permeable mirror; Patent of invention (application number 98802979.0) discloses a laser scanning confocal micro-scope system, measure Jiao as the time can be separating from defocused image at Jiao's picture, thereby not only reduced systematic error but also reduced statistical error.Patent of invention (application number 99113633.0) discloses a kind of self-align fiber-optic confocal scanning microscope that has, and comprises fiber-optic confocal scanning microscope and accurate confocal small field of view microscope.That is to say that a microscope of this invention has two kinds of microscopical functions, the function of existing microscan tomography, the function of imaging once promptly is to carry out self-align function to measuring target again.Yet above-mentioned confocal scan microscope all has a shortcoming that is difficult to overcome: image taking speed is too slow.In these confocal scan microscopes, each constantly can only obtain a picture element, wishes to get a complete image, needs pointwise to finish the scanning of both direction.Because the confocal scan microscope imaging resolution is high, in order to obtain high-resolution image, its sweep velocity is difficult to improve.Therefore, image taking speed is slow, just becomes important disadvantages of confocal scan microscope.Particularly be used for biomedicine in bulk measurement the time, image taking speed has seriously limited the application of confocal scan microscope slowly.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of laser confocal scanning microscope system is provided.
In order to improve the image taking speed of confocal scan microscope, technical scheme of the present invention is: the formation of laser-confocal scanning microscopic imaging apparatus comprises: LASER Light Source, object lens, semi-transparent semi-reflecting lens, imaging len, slit, enlarging objective and photoelectric sensor, after photoelectric sensor changes into data with the light signal of receiving, send data collector, entering computing machine handles, LASER Light Source provides illumination through collimator objective to semi-transparent semi-reflecting lens, system adopts confocal arrangement, the object of both having desired imaging is on the focal line of object lens, photoelectric sensor is on the focal line of imaging len, the light beam that LASER Light Source is sent is through collimator objective, after seeing through semi-transparent semi-reflecting lens, become a focal line through object lens in the internal focus of desiring imaging object, after the object tissue reflection that is in focal line, pass through object lens again, by semi-transparent semi-reflecting mirror reflection, be focused into a linear beam through imaging len, behind slit, be imaged on the photoelectric sensor by enlarging objective, described object lens are made one-dimensional scanning vertically or radially by additional control device control; The object of desiring imaging can be on the focus of object lens; Photoelectric sensor also can be on the focus of imaging len; Described object lens, imaging len adopt two gummed cylindrical mirrors; Described photoelectric sensor adopts line array CCD device or other linear some array photoelectric sensor; LASER Light Source adopts the laser instrument of infrared band or the laser instrument of visible light wave range (as red light).
The present invention can make the speed of confocal scan microscope improve hundred times, can simplify the structure of confocal scan microscope simultaneously, reduces volume, has expanded the usable range of confocal scan microscope widely.
Description of drawings
Fig. 1 is the system chart of confocal laser scanning microscope.Wherein: the 1st, object lens; The 2nd, semi-transparent semi-reflecting lens; The 3rd, collimator objective; The 4th, LASER Light Source; The 5th, imaging len; The 6th, slit; The 7th, enlarging objective; The 8th, photoelectric sensor; The 9th, data collector; The 10th, computing machine.
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail:
Fig. 1 is the system chart of confocal laser scanning microscope.
Principle of work of the present invention is as follows: LASER Light Source 4 is the laser instrument of near-infrared band, the beams of narrow-band light that it sends through collimator objective 3 be extended to 1 millimeter to several millimeters of diameter evenly, parallel beam, be split into the two-way light beam by semi-transparent semi-reflecting lens 2: the one tunnel need not through semi-transparent semi-reflecting lens 2 beam reflected; After another road light beam sees through semi-transparent semi-reflecting lens 2, become a focal line through object lens 1 in the internal focus of observed object, after the observed object reflection that is in focal line, pass through object lens 1 again, reflect by semi-transparent semi-reflecting lens 2, through imaging len 5, behind the slit 7, be imaged on the photoelectric sensor by enlarging objective.Because the pixel on the photoelectric sensor have one-to-one relationship with the observing object body image that is in focal line, therefore, can obtain being in hundreds if not thousands of pixels of observed object of focal line this moment.Along with object lens 1 axially or move radially, can obtain the high-definition picture of observed object.Owing to only need to do scanning motion, so this device can high speed imaging in a direction.The signal of photoelectric sensor output is sent into computing machine 10 through data collector 9, through obtaining the image of observed object after the COMPUTER CALCULATION.
Claims (7)
1. laser-confocal scanning microscopic imaging apparatus, its formation comprises: LASER Light Source, object lens, semi-transparent semi-reflecting lens, imaging len, slit, enlarging objective and photoelectric sensor, after photoelectric sensor changes into data with the light signal of receiving, send data collector, entering computing machine handles, LASER Light Source provides illumination through collimator objective to semi-transparent semi-reflecting lens, it is characterized in that, system adopts confocal arrangement, the object of both having desired imaging is on the focal line of object lens, photoelectric sensor is on the focal line of imaging len, the light beam that LASER Light Source is sent through behind the semi-transparent semi-reflecting lens, becomes a focal line through object lens in the internal focus of desiring imaging object through collimator objective, after the object tissue reflection that is in focal line, pass through object lens again, by semi-transparent semi-reflecting mirror reflection, be focused into a linear beam through imaging len, behind slit, on photoelectric sensor, described object lens are made one-dimensional scanning vertically or radially by additional control device control by the enlarging objective imaging.
2. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that, system adopts confocal arrangement, and the object of desire imaging is on the focus of object lens.
3. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that, system adopts confocal arrangement, and photoelectric sensor is on the focus of imaging len.
4. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that: described object lens adopt two gummed cylindrical mirrors.
5. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that: described imaging len adopts two gummed cylindrical mirrors.
6. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that: described photoelectric sensor adopts the line array CCD device.
7. laser-confocal scanning microscopic imaging apparatus according to claim 1 is characterized in that: described light source adopts the laser instrument of infrared band.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021044538A CN1170183C (en) | 2002-03-19 | 2002-03-19 | High-speed laser-confocal scanning microscopic imaging apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021044538A CN1170183C (en) | 2002-03-19 | 2002-03-19 | High-speed laser-confocal scanning microscopic imaging apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1369701A CN1369701A (en) | 2002-09-18 |
| CN1170183C true CN1170183C (en) | 2004-10-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB021044538A Expired - Fee Related CN1170183C (en) | 2002-03-19 | 2002-03-19 | High-speed laser-confocal scanning microscopic imaging apparatus |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100439959C (en) * | 2007-04-17 | 2008-12-03 | 天津大学 | Amplification imaging vision positioning device for IC package |
| US8785180B2 (en) | 2007-06-18 | 2014-07-22 | Shanghai Guoqiang Bioengineering Equipment Co., Ltd. | Biochemical reactor |
| CN201047885Y (en) * | 2007-06-18 | 2008-04-16 | 上海国强生化工程装备有限公司 | Online cell microscopic visualizer |
| CN101634747B (en) * | 2008-07-23 | 2012-01-11 | 中国科学院半导体研究所 | High-resolution confocal microscope |
| EP2517064A2 (en) * | 2009-12-22 | 2012-10-31 | Miao Zhang | Illumination methods and systems for improving image resolution of imaging systems |
| CN103954219B (en) * | 2014-04-08 | 2016-09-21 | 天津大学 | Two dimension strain line dynamic photoelectric aims at interferometric measuring means |
| CN106482663A (en) * | 2016-12-10 | 2017-03-08 | 巫献 | Based on 3-D scanning rifle in the hand-held cavity of common focusing principle |
| CN108061964B (en) * | 2017-10-25 | 2020-03-27 | 中国科学技术大学 | High-speed three-dimensional microscopic imaging device and method for large sample |
| CN108828625A (en) * | 2018-08-27 | 2018-11-16 | 安徽科创中光科技有限公司 | The device and method of one seed sand formula theorem imaging laser radar inverting atmospheric visibility |
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2002
- 2002-03-19 CN CNB021044538A patent/CN1170183C/en not_active Expired - Fee Related
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