CN211718616U - Simple fluorescence microscope - Google Patents
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- CN211718616U CN211718616U CN202020436293.9U CN202020436293U CN211718616U CN 211718616 U CN211718616 U CN 211718616U CN 202020436293 U CN202020436293 U CN 202020436293U CN 211718616 U CN211718616 U CN 211718616U
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Abstract
The utility model relates to a simple and easy fluorescence microscope belongs to the microscopic imaging technology field. The light source, the lens and the excitation end optical filter are sequentially arranged to form a first light path, the objective lens, the dichroic mirror, the collection end optical filter, the convex lens, the concave lens and the camera of the smart phone are sequentially arranged to form a second light path, the first light path and the second light path are mutually perpendicular and intersect at the dichroic mirror, and the biological sample to be observed is placed below the objective lens. The simple fluorescence microscope device adopts the photoelectric components and the matched optical-mechanical components which are conventional photoelectric devices, thereby greatly reducing the structural complexity of the system and greatly reducing the equipment cost. The camera of the smart phone is used for collecting images, so that the stereoscopic effect is realized, the system is simple and compact in structure, and the system cost is reduced. The simple fluorescence microscope can promote the application of the fluorescence microscope in biomedical teaching and research.
Description
Technical Field
The utility model relates to a simple and easy fluorescence microscope belongs to the microscopic imaging technology field.
Background
Microscopic imaging currently has important applications in biomedical research, new material characterization, and other fields. The conventional optical microscope performs imaging based on physical quantities such as light absorption, phase gradient, and birefringence of a sample, and generally has a disadvantage of low contrast. Whereas fluorescence microscopy images the spatiotemporal distribution of a single molecular species by exploiting the property of the sample to emit fluorescence. Fluorescence is light emitted in the process that when molecules are excited by light, electrons are transited from a ground state to an excited state, and then return to the ground state after relaxation. Due to the stokes shift between the excitation spectrum and the radiation spectrum of the fluorescent substance, the emitted fluorescence can be easily filtered out with a filter. After filtering out the excitation light, only the fluorescence signal emitted by the sample is observed, which can significantly improve the imaging contrast.
The fluorescence microscopic imaging has the advantages of specific labeling, real-time dynamic imaging of living cells and the like, and becomes a necessary technical means for the current biological science research. The fluorescent microscopic imaging can be used for researching the structure and the dynamic change of a specific cell or an organelle, and can also be used for researching the physiological dynamic process based on a calcium ion or voltage sensitive fluorescent molecule. For different cells or organelles, fluorescent proteins with different spectral characteristics are respectively used for specific labeling, and the dynamic interaction between the fluorescent proteins can be researched.
However, the existing commercial fluorescence microscope has a complex structure and high price, and is not beneficial to the wide application of the fluorescence microscope. Reducing the cost and complexity of fluorescence microscopes would help advance biomedical research.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a simple fluorescence microscope device, which adopts simple photoelectric component design and assembly to form a simple fluorescence microscope so as to reduce the structural complexity of the system; and replace eyepiece or camera through the camera of smart mobile phone, reduce equipment cost, it is more nimble convenient on the post processing of operation and image simultaneously.
The utility model provides a simple and easy fluorescence microscope, the realization mode of total two kinds of isotructures, wherein:
the first simple fluorescence microscope comprises a light source, a lens, an excitation end optical filter, a dichroic mirror, an objective lens, a collection end optical filter, a convex lens, a concave lens and a smart phone; the light source, the lens and the excitation end optical filter are sequentially arranged to form a first light path, the objective lens, the dichroic mirror, the collection end optical filter, the convex lens, the concave lens and the camera of the smart phone are sequentially arranged to form a second light path, the first light path and the second light path are mutually perpendicular and intersect at the dichroic mirror, and the biological sample to be observed is arranged below the objective lens.
The second simple fluorescence microscope comprises a light source, a lens, an excitation end optical filter, a dichroic mirror, an objective lens, a collection end optical filter, a convex lens, a concave lens, a beam splitting reflector, a left reflector, a right reflector and a smart phone, wherein the smart phone comprises two cameras; the light source, the lens and the excitation end optical filter are sequentially arranged to form a first optical path, the objective lens, the dichroic mirror, the collection end optical filter, the convex lens, the concave lens, the beam splitting reflector and the smart phone are sequentially arranged to form a second optical path, the first optical path and the second optical path are mutually perpendicular and intersect at the dichroic mirror, the normal directions of two reflecting surfaces of the beam splitting reflector respectively form an angle of 45 degrees with the second optical path, the normal directions of the left reflector and the right reflector respectively form an angle of 45 degrees with the second optical path, the left reflector and the right reflector are symmetrical relative to the second optical path, and a biological sample to be observed is placed below the objective lens.
The utility model provides a simple and easy fluorescence is micro-device, its advantage:
(1) the utility model discloses a microscopic device of simple and easy fluorescence, its photoelectric component who adopts and supporting ray apparatus subassembly are conventional photoelectric device, can directly purchase from the photoelectric device supplier, consequently greatly reduced the structure complexity of system to the system cost has been reduced.
(2) The utility model discloses an image is gathered to the camera that has used smart mobile phone among the simple and easy fluorescence microscopic device, need not use eyepiece or camera, utilizes smart mobile phone's two cameras, realizes the stereoscopic effect, consequently makes simple structure, the compactness of system, has greatly reduced equipment cost.
(3) Use the utility model discloses a simple and easy fluorescence microscope can obtain the wide visual field of waiting to observe biological sample, high resolution, have the fluorescence image of stereoscopic effect, and system simple structure, compactness, and the ray apparatus component easily obtains, can impel the application of fluorescence microscope in biomedical teaching, research.
Drawings
Fig. 1 is a light path diagram of a simple fluorescence microscope according to the present invention.
Fig. 2 is an optical diagram of another structure of the simple fluorescence microscope of the present invention.
In fig. 1-2, 1 is a light source, 2 is a lens, 3 is an excitation-side filter, 4 is a dichroic mirror, 5 is an objective lens, 6 is a biological sample to be observed, 7 is a collection-side filter, 8 is a convex lens, 9 is a concave lens, 10 is a smartphone, 11 is a beam splitting mirror, 12 is a left mirror, and 13 is a right mirror. The arrows indicate the direction of the light and the dashed lines indicate the light beams.
Detailed Description
The utility model provides an use simple and easy fluorescence microscope of smart mobile phone collection image has the implementation of two kinds of isotructures, wherein:
the first simple fluorescence microscope is structurally shown in fig. 1 and comprises a light source 1, a lens 2, an excitation end optical filter 3, a dichroic mirror 4, an objective lens 5, a collection end optical filter 7, a convex lens 8, a concave lens 9 and a smart phone 10; the light source 1, the lens 2 and the excitation end optical filter 3 are sequentially arranged to form a first light path, the objective lens 5, the dichroic mirror 4, the collection end optical filter 7, the convex lens 8, the concave lens 9 and the camera of the smart phone 10 are sequentially arranged to form a second light path, the first light path and the second light path are mutually perpendicular and intersect at the dichroic mirror 4, and a biological sample to be observed is arranged below the objective lens 5.
The second simple fluorescence microscope is structurally shown in fig. 2, and comprises a light source 1, a lens 2, an excitation end optical filter 3, a dichroic mirror 4, an objective lens 5, a collection end optical filter 7, a convex lens 8, a concave lens 9, a beam splitting mirror 11, a left reflecting mirror 12, a right reflecting mirror 13 and a smart phone 10, wherein the smart phone 10 comprises two cameras; the light source 1, the lens 2 and the excitation end optical filter 3 are sequentially arranged to form a first optical path, the objective lens 5, the dichroic mirror 4, the collection end optical filter 7, the convex lens 8, the concave lens 9, the beam splitting reflector 11 and the smart phone 10 are sequentially arranged to form a second optical path, the first optical path and the second optical path are mutually perpendicular and intersect at the dichroic mirror 4, the normal directions of two reflecting surfaces of the beam splitting reflector 11 form an angle of 45 degrees with the second optical path respectively, the normal directions of the left reflector 12 and the right reflector 13 form an angle of 45 degrees with the second optical path respectively, the left reflector 12 and the right reflector 13 are symmetrical relative to the second optical path, and a biological sample to be observed is placed below the objective lens 5.
The working principle and the working process of the simple fluorescence microscope provided by the utility model are described in detail below with the accompanying drawings:
in the simple fluorescence microscope shown in fig. 1, the light source 1 is an LED light source, and the lens 2 and the objective lens 5 together form kohler illumination for generating excitation light for uniform illumination on the biological sample 6 to be measured. Since the light source 1 is generally a broad spectrum light source, excitation light suitable for exciting a fluorescent protein labeled in a biological sample to be observed needs to be screened out by the excitation-side optical filter 3. After transmitting through the excitation-end optical filter 3, excitation light is reflected by the dichroic mirror 4 to enter the objective lens 5 and is focused on the biological sample 6 to be observed, a fluorescent substance in the biological sample 6 to be observed is excited, and a fluorescent signal is emitted, wherein the process is an excitation process of fluorescence. The fluorescence signal emitted by the biological sample 6 to be observed is then collected by the objective 5, this fluorescence signal being termed emitted light. After transmitting through the dichroic mirror 4 and the collection end optical filter 7, the emitted light is contracted through the convex lens 8 and the concave lens 9, the contracted emitted light is directly imaged through the camera of the smart phone 10 and is recorded in the smart phone 10, and the image obtained by the smart phone 10 is the fluorescence image of the biological sample to be observed, which is the collection process of the fluorescence. The dashed lines in fig. 1 represent light beams.
In the simple fluorescence microscope shown in FIG. 1, the focal length of the convex lens 8 is set to | f1The focal length of the concave lens 9 is | f2I, the exit pupil diameter of the objective lens 5 is D1The entrance pupil aperture of the camera of the smart phone 10 is D2The beam reduction ratio of the emitted lightExample M may be expressed as M ═ f2|/|f1|＝D2/D1The distance between the concave lens 9 and the convex lens 8 is | f1|-|f2L. Therefore, the convex lens 8 and the concave lens 9 are selected according to the actual conditions of the objective lens 5 and the camera of the smart phone 10.
In the simple fluorescence microscope shown in fig. 2, the selected smartphone 10 includes two cameras, and compared with the simple fluorescence microscope shown in fig. 1, a beam splitting mirror 11, a reflecting mirror 12, and a reflecting mirror 13 are added. Wherein the excitation process of fluorescence is the same as shown in figure 1. After the biological sample 6 to be observed emits a fluorescent signal, the fluorescent signal emitted by the biological sample 6 to be observed is collected by the objective lens 5, and this fluorescent signal is named emission light. The emitted light is transmitted through the dichroic mirror 4 and the collection end filter 7, then is condensed by the convex lens 8 and the concave lens 9, and the condensed emitted light is split into two beams of light with equal energy by the beam splitting mirror 11. One beam of light is reflected by the reflector 12 and then recorded by a camera of the smart phone 10; the other beam of light is reflected by the mirror 13 and recorded by the other camera of the smartphone 10, which is the process of collecting fluorescence.
The dashed lines in fig. 2 represent light beams.
In the simple fluorescence microscope shown in fig. 2, the beam splitting mirror 11 is used to split the spatial frequency spectrum information of the emitted light, and the images obtained by the two cameras are respectively the fluorescence images of the biological sample to be observed under different viewing angles. Setting the focal lengths of two cameras of the selected smart phone 10 with the two cameras to be f, and setting the interval between the two cameras to be b; by combining a binocular vision processing algorithm and judging the parallax d between corresponding pixel points in the images formed by the two cameras, the depth information of each pixel point in the images can be obtained
Therefore, the simple fluorescence microscope with the structure can obtain the effect of binocular stereoscopic vision. The beam reduction ratio of the emitted light in the simple fluorescence microscope of this structure, and the positional relationship between the convex lens 8 and the concave lens 9 are the same as those in the first structure, and are determined according to the objective lens5 and a camera of the smart phone 10 selects a proper convex lens 8 and a proper concave lens 9. When the reflector 12 and the reflector 13 are placed, it is required to ensure that the optical paths of two beams of light split by the beam splitting reflector to the camera are the same respectively; the distance between the reflector 12 and the reflector 13 is equal to the distance between the two cameras of the smartphone 10, and should be adjusted according to the smartphone 10 used.
The utility model discloses a simple and easy fluorescence microscope, for satisfying kohler's illumination under different excitation conditions, the distance is adjustable between light source 1 wherein, lens 2, objective 5, but the position relation is unchangeable. The excitation filter 3, the dichroic mirror 4 and the collection filter 7 are selected according to the excitation light and emission light wavelengths of the fluorescent substance labeled in the biological sample 6 to be observed.
The biological sample 6 to be observed is placed on the three-dimensional precise displacement table, so that the three-dimensional position of the biological sample can be accurately adjusted. In performing the imaging observation, the biological sample 6 to be observed should be accurately placed at the Working Distance (WD) of the objective lens 5.
Claims (2)
1. A simple fluorescence microscope is characterized by comprising a light source, a lens, an excitation end optical filter, a dichroic mirror, an objective lens, a collection end optical filter, a convex lens, a concave lens and a smart phone; the light source, the lens and the excitation end optical filter are sequentially arranged to form a first light path, the objective lens, the dichroic mirror, the collection end optical filter, the convex lens, the concave lens and the camera of the smart phone are sequentially arranged to form a second light path, the first light path and the second light path are mutually perpendicular and intersect at the dichroic mirror, and the biological sample to be observed is arranged below the objective lens.
2. A simple fluorescence microscope is characterized by comprising a light source, a lens, an excitation end optical filter, a dichroic mirror, an objective lens, a collection end optical filter, a convex lens, a concave lens, a beam splitting reflector, a left reflector, a right reflector and a smart phone, wherein the smart phone comprises two cameras; the light source, the lens and the excitation end optical filter are sequentially arranged to form a first optical path, the objective lens, the dichroic mirror, the collection end optical filter, the convex lens, the concave lens, the beam splitting reflector and the smart phone are sequentially arranged to form a second optical path, the first optical path and the second optical path are mutually perpendicular and intersect at the dichroic mirror, the normal directions of two reflecting surfaces of the beam splitting reflector respectively form an angle of 45 degrees with the second optical path, the normal directions of the left reflector and the right reflector respectively form an angle of 45 degrees with the second optical path, the left reflector and the right reflector are symmetrical relative to the second optical path, and a biological sample to be observed is placed below the objective lens.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113781848A (en) * | 2021-08-14 | 2021-12-10 | 西安电子科技大学 | Optical projection tomography device, system and method based on smart phone |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113781848A (en) * | 2021-08-14 | 2021-12-10 | 西安电子科技大学 | Optical projection tomography device, system and method based on smart phone |
| CN113781848B (en) * | 2021-08-14 | 2023-03-07 | 西安电子科技大学 | Optical projection tomography device, system and method based on smart phone |
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