CN101539456B - General hyperspectral imaging component - Google Patents
General hyperspectral imaging component Download PDFInfo
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- CN101539456B CN101539456B CN2009100498976A CN200910049897A CN101539456B CN 101539456 B CN101539456 B CN 101539456B CN 2009100498976 A CN2009100498976 A CN 2009100498976A CN 200910049897 A CN200910049897 A CN 200910049897A CN 101539456 B CN101539456 B CN 101539456B
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Abstract
The invention discloses a general hyperspectral imaging component, comprising a C-Mount interface, a collimator, a dispersion unit, a converging mirror, CCD, a data acquisition and control module, communication cables and a computer. The invention has the following characteristics: the C-Mount interface, the collimator, the dispersion unit, the converging mirror and CCD are in light path connection in turn; the reflected light or the transmitted light of optical instruments is introduced to the collimator through the C-Mount interface, split in the dispersion unit and image on the CCD by the converging mirror. An output signal cable of the CCD is connected with the data acquisition and control module, a control cable of the data acquisition and control module is connected with the dispersion unit and the data acquisition and control module is also connected with the computer. By the control of the data acquisition and control module, the length of incident light is changed by the dispersion unit, the image data is collected by the CCD and the hyperspectral image data is obtained in visual field region of optical instruments. The invention can be coupled with common optical instruments to extend the utilization range of optical instruments.
Description
Technical field
The present invention relates to a kind of light spectrum image-forming assembly that can be coupled with microscope, general camera, camera.It specifically is a kind of general hyperspectral imaging component.Be used for fields such as biological sample analysis, clinical diagnosis, material sign.
Background technology
In life science and material science research field, the optical imagery instrument is the important means of observation and analyzing samples.These optical imagery instruments comprise biological microscope, fluorescent microscope, stereomicroscope, inverted microscope, metaloscope etc. at microcosmic imaging side face; Comprise monochromatic digital image-forming instrument, colored digital imager, infrared thermoviewer, pickup camera etc. at macroscopical imaging side face.These optical imagery instruments can only carry out gray scale or colour imaging to detecting target, use image processing and pattern recognition that target is carried out analysis on the morphosis then.Along with the needs of the development and the research of technology, when sample is carried out check and analysis, except will carrying out the morphological analysis, also require sometimes its biological chemistry is changed and composition carries out Quantitative study to it, promptly target is carried out the chemical imaging analysis.
At life science, existing biochemical analysis method is by means of all ingredients on the one hand, is to use special-purpose functional image equipment on the one hand.These special-purpose functional image functions of the equipments are single relatively and cost an arm and a leg, and dirigibility is relatively poor.The Hyper spectral Imaging technology is applied to the remote sensing field at first, at aspects such as geology, agricultural, vegetation, environment, city, military affairs, the hydrology, atmosphere good application is arranged all.Analysis as if this technology being applied to sample then can realize form and biochemical analysis simultaneously, carries out the description of qualitative, quantitative and location to detecting target, thereby realizes the early diagnosis to some pathological change.Population health level and people's living standard are significant for improving.At material science, can only obtain the technology of object construction information compares with ordinary optical microscope, Electronic Speculum, AFM etc., the light spectrum image-forming technology not only can be analyzed with quantification the surface structure information of material and describe, can also accurately discern and analyze the constituent of material, and combined with intelligent discriminance analysis algorithm realizes the distribution and the situation of change of different compositions, and material is carried out more meticulous sign.
Though the light spectrum image-forming technology has good prospects for application in fields such as life science and material science, but owing to existing optical spectrum imagers all are dedicated system of developing at a certain concrete goal in research, can not with these fields in be coupled at the optical detection apparatus that uses, so this technology is not also in these field widespread uses.
Summary of the invention
The objective of the invention is to overcome the light spectrum image-forming technology uses in fields such as life science and material science and has the problem that optical instrument combines difficulty now, provide a kind of standard interface that has, the general hyperspectral imaging component that can be well is coupled and uses with existing optical detecting instrument such as microscope, camera etc.This general hyperspectral imaging component uses the standard interface of C-Mount as imaging, use general chromatic dispersion unit that the reflected light or the transmitted light of optical instrument are carried out beam split, use charge-coupled device (CCD) to carry out the record of data, once gather the information that promptly can obtain observed object image and spectrum two aspects simultaneously, handle through the Intelligent Recognition analysis software again, promptly can obtain than existing optical detecting instrument more sample information.By using this general hyperspectral imaging component, can expand existing optical detecting instrument and use widely.
The object of the present invention is achieved like this:
A kind of general hyperspectral imaging component, comprise C-Mount interface, collimating mirror, chromatic dispersion unit, convergent mirror, CCD, data acquisition and control module, telecommunication cable and computing machine, characteristics are that C-Mount interface, collimating mirror, chromatic dispersion unit, convergent mirror, CCD become light path to connect successively; The reflected light of optical instrument or transmitted light arrive the chromatic dispersion unit through collimating mirror after being coupled through the C-Mount interface, and the light of process chromatic dispersion unit chromatic dispersion images on the CCD through behind the convergent mirror; The data output cable of CCD is connected to data acquisition and control module, and the umbilical cable of data acquisition and control module is connected to the chromatic dispersion unit, the beam split work of control chromatic dispersion unit; The output cable of data acquisition and control module is connected to computing machine, and the ultraphotic spectrum data transmission of gathering is handled to computing machine.
By the image of continuous acquisition optical instrument, can obtain hyperspectral image data in the optical instrument visual field at last, i.e. the image cube at different wave length.This image cube had both comprised the view data of optical instrument institute observation sample, had also comprised its spectral information.These data transmission are handled and analyzed to computing machine, promptly can realize sample is carried out form and biochemical analysis simultaneously, thereby expanded the function of existing optical detecting instrument.
Above-mentioned optical instrument is the detecting instrument with C-Mount standard interface, as all kinds of microscopes, industrial camera etc.; Described chromatic dispersion unit can be elements such as acousto-optic tunable wave filter (AOTF), liquid crystal tunable filter (LCTF), arc prism spectrometer or grating; Be connected data cables such as to use serial communication RS232, general-purpose serial bus USB or netting twine between data acquisition and control module and the computing machine.
General hyperspectral imaging component of the present invention has the C-Mount standard interface, can be coupled with the universal optical instrument, has expanded its check and analysis function.Have good versatility and practicality.
Description of drawings
Fig. 1 is a structural representation of the present invention
Embodiment
Provide one of the present invention embodiment preferably according to Fig. 1 below, in order to architectural feature of the present invention, technical feature and function point to be described.
Consult Fig. 1, C-Mount interface 1, collimating mirror 2, chromatic dispersion unit 3, convergent mirror 4, CCD 5 become light path to connect successively in the present embodiment, the reflected light of external optical instrument 9 or transmitted light import into to collimating mirror 2 through C-Mount interface 1, after 3 beam split of chromatic dispersion unit, image on the CCD 5 through convergent mirror 4.The output cable of CCD 5 is connected on data acquisition and the control module 6, gathers and recording image data, and the umbilical cable of data acquisition and control module 6 is connected to the control port of chromatic dispersion unit 3, controls according to the needs of the images acquired throwaway unit 3 of checking colors.Data acquisition and control module 6 are connected to computing machine 8 by telecommunication cable 7, and under the support of driver, computing machine 8 can the control data collection and the work of control module 6 and read the view data of its collection.
In the present embodiment, the telecommunication cable between data acquisition and control module 6 and the computing machine 8 adopts the USB line, and chromatic dispersion unit 3 adopts the acousto-optic tunable wave filter (AOTF) of Brimrose, and CCD 5 uses the monochromatic CCD of the KP-F120 of Hitachi.During instrument work, the reflected light of sample or transmitted light enter C-Mount interface 1 through optical instrument 9, arrive chromatic dispersion unit 3 through collimating mirror 2, data acquisition makes reflected light or transmitted light be in different wavelength with control module 6 control chromatic dispersion unit 3, under each fixed wave length, gather the output data of CCD 5, promptly can obtain the hyperspectral image data of optical instrument 9 field of view at last.
After the hyperspectral image data of having obtained optical instrument 9 field of view, the user promptly can write corresponding hyperspectral image data analysis software according to the needs of research on computing machine 8, the data of obtaining are carried out Intelligent Recognition and analysis.As the single band image being shown; The curve of spectrum of each picture element correspondence can be depicted; Can realize classification etc. by the difference that compares between these curves of spectrum.
The present invention can be coupled with the ordinary optical detecting instrument with C-Mount standard interface, under the condition of not changing former optical instrument, realization is to the ultraphotic spectrum data acquisition of optical instrument institute observation sample, not only can on form, analyze sample, can also detect sample from biochemical angle, thereby the more sample information of horn of plenty is provided, has expanded the function of original optical detecting instrument.The present invention has good versatility, and the optical instrument coupling with fields such as life science and material science can greatly improve instrument performance, has important application value.
Claims (3)
1. general hyperspectral imaging component, comprise C-Mount interface (1), collimating mirror (2), chromatic dispersion unit (3), convergent mirror (4), CCD (5), data acquisition and control module (6), telecommunication cable (7) and computing machine (8), it is characterized in that C-Mount interface (1), collimating mirror (2), chromatic dispersion unit (3), convergent mirror (4), CCD (5) become light path to connect successively; The output signal cable of CCD (5) connects data acquisition and control module (6), and data acquisition is connected chromatic dispersion unit (3) and computing machine (8) with control module (6); Wherein C-Mount interface (1) is a standard interface, is coupled with the optical instrument with this interface (9).
2. general hyperspectral imaging component according to claim 1 is characterized in that described chromatic dispersion unit (3) is acousto-optic tunable wave filter (AOTF) or liquid crystal tunable filter (LCTF) or arc prism spectrometer or grating.
3. general hyperspectral imaging component according to claim 1 is characterized in that described telecommunication cable (7) is serial communication RS232 cable or general-purpose serial bus USB cable or netting twine data cable.
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CN2009100498976A CN101539456B (en) | 2009-04-24 | 2009-04-24 | General hyperspectral imaging component |
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Cited By (1)
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TWI580925B (en) * | 2015-03-06 | 2017-05-01 | Snu精密股份有限公司 | Method and apparatus for measuring thickness using color camera |
Families Citing this family (5)
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CN103017902B (en) * | 2012-11-30 | 2014-12-10 | 南京航空航天大学 | Compact spectrum collecting device and collecting method |
CN103728019B (en) * | 2013-12-19 | 2015-10-21 | 南京邮电大学 | A kind of spectral measurement device based on acoustooptic modulation and spectral measurement method |
CN104713648A (en) * | 2015-04-08 | 2015-06-17 | 四川双利合谱科技有限公司 | Full-wave-band achromatism filter type spectral camera |
DE102017100904B4 (en) * | 2017-01-18 | 2023-01-19 | Carl Zeiss Microscopy Gmbh | Image conversion module for a microscope and microscope |
CN109489816B (en) * | 2018-10-23 | 2021-02-26 | 华东师范大学 | Microscopic hyperspectral imaging platform and large-area data cube acquisition method |
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TWI580925B (en) * | 2015-03-06 | 2017-05-01 | Snu精密股份有限公司 | Method and apparatus for measuring thickness using color camera |
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