CN102226756A - Acousto-optic spectrum imaging microscopic system - Google Patents
Acousto-optic spectrum imaging microscopic system Download PDFInfo
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- CN102226756A CN102226756A CN2011100795062A CN201110079506A CN102226756A CN 102226756 A CN102226756 A CN 102226756A CN 2011100795062 A CN2011100795062 A CN 2011100795062A CN 201110079506 A CN201110079506 A CN 201110079506A CN 102226756 A CN102226756 A CN 102226756A
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Abstract
The invention relates to an acousto-optic spectrum imaging microscopic system which can realize the taking of high resolution spectral images of small samples like biological samples, chemical samples and metal structures in visible lights and near infrared bands. Thus, a foundation is laid for analysis of sample appearance and property. The invention is characterized in that: optical microscopic imaging technology and acousto-optic modulating light splitting technology are combined organically, with an acousto-optic tunable filter (AOTF) as the core light splitting component, and by means of a bulk grating structure formed during the propagation of supersonic waves in an acousto-optic crystal, incident lights are subjected to Bragg diffraction, thus achieving the light splitting effect. In addition, the supersonic wave frequency of the system and the wavelength of diffracted lights are in one-to-one correspondence, so continuous spectrum scanning can be realized by changing the supersonic wave frequency. With the advantages of small volume, light weight, all solid state, no moving part, good aseismatic performance, excellent environment adaptiveness and the like, the acousto-optic spectrum imaging microscopic system can be applied widely in the fields of life science, nanometer material, forensic science, etc.
Description
Technical field
The present invention relates to a kind of acousto-optic light spectrum image-forming microscopic system,, can carry out the spectrum picture collection of microcell, obtain the shape characteristic and the spectral characteristic of sample to be tested simultaneously when spectral range during at visible and near-infrared band.Application comprises life science, nano material, medical jurisprudence etc.
Background technology
Along with people further improve the requirement of micro-Detection Techniques, the tradition microtechnic has run into technical bottleneck: when light source is white light, the light of different wave length passes lens in the white light, because the difference of refractive index, the light imaging can not be converged, and present the ring of light that has colour edging, promptly produce aberration, and reduce the sharpness of lens imaging; And when adopting monochromatic source or adding optical filter, then can not embody the spectral characteristic of material.This is restricted in the application of some occasion with regard to giving microscopic system.Acousto-optic light spectrum image-forming microscopic system is the optical instrument that micro-imaging technology and spectrographic detection technology are combined, can take the spectrum picture information of microcosmic target, being a great innovation of micro-Detection Techniques, is human observation and the another powerful measure of being familiar with microworld.Based on the acousto-optic light spectrum image-forming microscopic system of AOTF have simple in structure, the spectrum conversion is fast.No moving-member, the reliability advantages of higher.
The present invention's acousto-optic light spectrum image-forming microscopic system mainly is made up of variable power optical microphotograph part, high resolution CCD imaging moiety, acousto-optic is adjustable beam split part and computer control and management system.The present invention utilizes the core parts of acousto-optic tunable filter as the beam split part, according to the acousto-optic interaction principle, relies on the variation of ultrasonic frequency that transmitted light wave progress row is selected, and is wherein ripe to the research of acousto-optic tunable filter.
AOTF is divided into monochromatic light with light source light spectrum among the present invention, and be divided into two bundles: a branch of lighting source as the transmission sample imaging by optical splitter (11), incide object under test on the cover glass by condenser (9) and objective table (8), its transmitted light beam incides on the high resolution CCD (1) through spectroscope (3), carries out image acquisition.Change ultrasonic frequency, optical wavelength also changes accordingly, has realized the scanning of object transmitted spectrum image.The lighting source of another Shu Zuowei reflection sample imaging, when object under test was nontransparent object (as metal), this light beam incided on the object under test, and its reflected light incides on the high resolution CCD through spectroscope (3).CCD is converted into electric signal with the monochromatic spectrum signal that collects, and is input in the computing machine and preserves.Said process is very similar with classical optical microscope, and this part technology can realize.
In sum, this invents related technology (as information management system, ultrasonic control system, image capturing system, beam splitting system and system building) maturation, and this invention can realize.
Description of drawings
Accompanying drawing 1 is the structural representation of acousto-optic light spectrum image-forming microscopic system;
Accompanying drawing 2 is structural representations of AOTF;
Accompanying drawing 3 is workflow diagrams of acousto-optic light spectrum image-forming microscopic system;
Accompanying drawing 4 is spectrum pictures of 500nm and two kinds of next piece bloodstain of wavelength of 700nm.
Embodiment
Provide embodiment with the spectrum picture of measuring the blood rbc smear:
The first step: experiment is prepared, and opens light source earlier, preheating, and whether reexamine other parts working properly;
Second step: selected target to be measured---a bloodstain places on the objective table;
The 3rd step: start computing machine, open control and management software;
The 4th step: select the object lens of suitable enlargement factor, the target to be measured that detects by an unaided eye is clear to target imaging to be measured;
The 5th step: utilize the computing machine output ultrasonic frequency corresponding, carry out beam split by AOTF with required wavelength;
The 6th step: observe target to be measured by eyepiece, if clear picture is carried out image acquisition with high resolution CCD, and carried out graphical analysis, processing by computing machine; If image blurring, then adjust focal length, observe again, up to clear picture again to its collection, analysis and processing.
Claims (7)
1. acousto-optic light spectrum image-forming microscopic system is characterized in that: mainly be made up of variable power optical microphotograph part, high resolution CCD imaging moiety, acousto-optic is adjustable beam split part and computer control and management system four parts.This acousto-optic light spectrum image-forming microscopic system adds AOTF as the beam split link based on optical microscope, illumination light is divided into different wave length scans, thereby realize micro-spectrogram picked-up.
2. variable power optical microphotograph part according to claim 1 is characterized in that: be made up of eyepiece (2), object lens (6), objective table (8), condenser (9), reflective mirror (10) and optical splitter (3).The enlargement factor of system is 100-1000 times.
3. high resolution CCD imaging moiety according to claim 1 is characterized in that: the detecting light spectrum scope of used planar array detector is 400nm～1000nm, and the spectral range of the spectrum camera of setting with the present invention conforms to.Its image resolution ratio must be greater than 1600H * 1280V.
4. the adjustable beam split part of acousto-optic according to claim 1 is characterized in that: adopt TeO
2As analyzing crystal, piezoelectric transducer is at TeO
2One end of crystal provides ultrasound wave, TeO
2The other end of crystal adds foamed sound absorber, and ultrasound wave is propagated to go the form of ripple in crystal.
5. TeO according to claim 4
2Analyzing crystal, it is characterized in that: regulate the ultrasonic frequency that is added on the crystal, corresponding lambda1-wavelength is diffracted into ± 1 order diffraction light, the gross energy of the zero order light of transmission and+1 order diffraction light absorbs with light barrier (12), reduce the interference of right-1 order diffraction light (13), and-1 order diffraction light is divided into two bundles by optical splitter (11), and a branch ofly be used for the transmission sample imaging, a branch ofly be used to reflect the sample imaging.
6. piezoelectric transducer according to claim 4 is characterized in that: input ultrasonic frequency scope is 20MHz～500MHz, is carried in TeO
2On the crystal, corresponding transmitted spectrum scope is 0.4 μ m～1 μ m.
7. computer control according to claim 1 and management system is characterized in that: export the ultrasonic frequency corresponding, act on piezoelectric transducer with required wavelength, and control high resolution CCD imaging moiety, gather the microspectrum image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011100795062A CN102226756A (en) | 2011-03-31 | 2011-03-31 | Acousto-optic spectrum imaging microscopic system |
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| CN2011100795062A CN102226756A (en) | 2011-03-31 | 2011-03-31 | Acousto-optic spectrum imaging microscopic system |
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| CN102226756A true CN102226756A (en) | 2011-10-26 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103900693A (en) * | 2014-02-18 | 2014-07-02 | 哈尔滨工业大学 | Difference snapshooting type imaging spectrometer and imaging method |
| CN105157831A (en) * | 2015-09-22 | 2015-12-16 | 中国科学院上海技术物理研究所 | Field diaphragm assembly for spectrometer adjustment test |
| CN107271442A (en) * | 2017-07-21 | 2017-10-20 | 黄宁 | A kind of medical test multipurpose test system |
| CN111024641A (en) * | 2019-12-30 | 2020-04-17 | 核工业北京地质研究院 | Short wave infrared microscopic rock mineral identification device |
| CN114689523A (en) * | 2022-03-25 | 2022-07-01 | 江苏省农业科学院 | System and method for optically detecting food-borne pathogenic bacteria |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5216484A (en) * | 1991-12-09 | 1993-06-01 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Real-time imaging spectrometer |
| CN201041514Y (en) * | 2007-06-14 | 2008-03-26 | 武汉大学 | Portable imaging spectrum instrument |
| CN201681208U (en) * | 2009-12-10 | 2010-12-22 | 中国科学院西安光学精密机械研究所 | AOTF optical system with image drift compensation |
-
2011
- 2011-03-31 CN CN2011100795062A patent/CN102226756A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5216484A (en) * | 1991-12-09 | 1993-06-01 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Real-time imaging spectrometer |
| CN201041514Y (en) * | 2007-06-14 | 2008-03-26 | 武汉大学 | Portable imaging spectrum instrument |
| CN201681208U (en) * | 2009-12-10 | 2010-12-22 | 中国科学院西安光学精密机械研究所 | AOTF optical system with image drift compensation |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103900693A (en) * | 2014-02-18 | 2014-07-02 | 哈尔滨工业大学 | Difference snapshooting type imaging spectrometer and imaging method |
| CN103900693B (en) * | 2014-02-18 | 2015-11-18 | 哈尔滨工业大学 | A kind of fast illuminated imaging spectrometer of difference and formation method |
| CN105157831A (en) * | 2015-09-22 | 2015-12-16 | 中国科学院上海技术物理研究所 | Field diaphragm assembly for spectrometer adjustment test |
| CN105157831B (en) * | 2015-09-22 | 2017-06-27 | 中国科学院上海技术物理研究所 | A kind of spectrometer debugs test field stop component |
| CN107271442A (en) * | 2017-07-21 | 2017-10-20 | 黄宁 | A kind of medical test multipurpose test system |
| CN111024641A (en) * | 2019-12-30 | 2020-04-17 | 核工业北京地质研究院 | Short wave infrared microscopic rock mineral identification device |
| CN114689523A (en) * | 2022-03-25 | 2022-07-01 | 江苏省农业科学院 | System and method for optically detecting food-borne pathogenic bacteria |
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Application publication date: 20111026 |