CN112143670A - 一种短乳杆菌8-2b及其应用 - Google Patents
一种短乳杆菌8-2b及其应用 Download PDFInfo
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- CN112143670A CN112143670A CN202010936554.8A CN202010936554A CN112143670A CN 112143670 A CN112143670 A CN 112143670A CN 202010936554 A CN202010936554 A CN 202010936554A CN 112143670 A CN112143670 A CN 112143670A
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- lactobacillus brevis
- aspergillus carbonarius
- aspergillus
- carbonarius
- fermentation product
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Abstract
本发明涉及微生物学领域，具体公开了一种短乳杆菌8‑2B及其应用。本发明的短乳杆菌(Lactobacillus brevis)8‑2B，其保藏编号为CGMCC No.20330。本发明的短乳杆菌(Lactobacillus brevis)8‑2B可高效抑制炭黑曲霉的生长和产毒。对炭黑曲霉的孢子萌发、芽管长度均产生明显抑制作用。在农产品保鲜、微生物制剂及其他抗菌相关领域具有较好的开发应用前景，为微生物源OTA抑制剂的进一步研制和开发提供了优良的基础菌株。
Description
技术领域
本发明涉及微生物学领域，具体地说，涉及一种短乳杆菌8-2B及其应用。
背景技术
赭曲霉毒素A(Ochratoxin A,OTA)主要是由曲霉菌属(Aspergillus)和青霉菌属(Penicillium)真菌产生的有毒次生代谢产物，广泛污染多种农产品，包括谷物、葡萄、坚果和香辛料等。炭黑曲霉(Aspergillus carbonarius)是造成农产品中OTA污染的主要来源，分布广泛。OTA作为一种天然污染物，在各类食品及饲料中的OTA污染情况严重。
OTA的毒性仅次于黄曲霉毒素，主要危及人和动物肾脏，同时对免疫系统也有毒性，还具有致畸、致癌、致突变等作用。目前对抑制炭黑曲霉和赭曲霉生长及OTA形成的方法可以归纳为3种：物理方法、化学方法和生物方法。物理方法有紫外线、γ-射线照射；化学方法有杀菌剂、二氧化硫、多酚类化合物和精油熏蒸；而生物方法为利用酵母、细菌或真菌抑制等。利用物理化学方法抑菌存在成本高、效率低、营养损失等缺点，因此利用高效、无毒无害的微生物来控制食品原料和加工过程中的炭黑曲霉的生长与产毒，已经成为研究热点。
发明内容
本发明的目的是提供一种能高效抑制炭黑曲霉生长及产OTA毒素的短乳杆菌及其应用。
为了实现本发明的目的，本发明的技术方案如下：
本发明的短乳杆菌(Lactobacillus brevis)8-2B是从北京波龙堡酒庄的葡萄表皮上分离得到的，其于2020年7月13日保藏在中国微生物菌种保藏管理委员会普通微生物中心(简称CGMCC，地址：北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所，邮编100101)，其分类命名为短乳杆菌Lactobacillus brevis，保藏编号为CGMCC No.20330。
进一步地，本发明提供所述短乳杆菌(Lactobacillus brevis)8-2B的发酵产物。
本发明提供一种菌剂，其含有所述的短乳杆菌(Lactobacillus brevis)8-2B或所述的发酵产物。
优选地，所述菌剂为固体菌剂或液体菌剂。
本发明还提供一种所述的短乳杆菌(Lactobacillus brevis)8-2B或所述的发酵产物或所述的菌剂在如下任一方面的应用：
(1)在抑制炭黑曲霉生长中的应用；
(2)在抑制炭黑曲霉生产赭曲霉毒素A中的应用；
(3)在农产品防霉中的应用，优选农产品为葡萄。
本发明还提供一种炭黑曲霉生长抑制剂，其含有上述短乳杆菌(Lactobacillusbrevis)8-2B或发酵产物。
一种抑制炭黑曲霉生产赭曲霉毒素A的抑制剂，其含有上述短乳杆菌(Lactobacillus brevis)8-2B或发酵产物。
一种农产品防霉剂，其含有上述短乳杆菌(Lactobacillus brevis)8-2B或发酵产物。
本发明的有益效果至少在于：
本发明的短乳杆菌(Lactobacillus brevis)8-2B可高效抑制炭黑曲霉的生长和产毒。对炭黑曲霉的孢子萌发、芽管长度均产生明显抑制作用。在农产品保鲜、微生物制剂及其他抗菌相关领域具有较好的开发应用前景，为微生物源OTA抑制剂的进一步研制和开发提供了优良的基础菌株。
附图说明
图1为基于16S rRNA基因序列采用临近法构建的Lactobacillus brevis和相近菌株的系统发育进化树；
图2为短乳杆菌8-2B抑制炭黑曲霉生长情况统计图；
图3为短乳杆菌8-2B抑制炭黑曲霉芽管长度情况统计图；其中，CK代表对照组，A、B、C、D分别所对应的短乳杆菌8-2B菌浓度为104、105、106和107cells/mL；
图4为短乳杆菌8-2B抑制炭黑曲霉孢子萌发情况统计图；其中，CK代表对照组，A、B、C、D分别所对应的短乳杆菌8-2B菌浓度为104、105、106和107cells/mL；
图5为炭黑曲霉菌丝的扫描电镜观察照片，显示短乳杆菌8-2B对炭黑曲霉菌丝的影响；
图6为炭黑曲霉细胞内部的透射电镜观察照片，显示短乳杆菌8-2B对炭黑曲霉细胞内部的影响；
图7为短乳杆菌8-2B抑制葡萄腐烂情况统计图；其中，
代表对照组，
代表实验组；
图8为短乳杆菌8-2B在葡萄上对炭黑曲霉的影响照片；图8A为对照组，图8B为实验组；
图9为短乳杆菌8-2B抑制葡萄腐烂情况统计图；其中，
代表对照组，
代表实验组。
具体实施方式
下面将结合实施例对本发明的优选实施方式进行详细说明。需要理解的是以下实施例的给出仅是为了起到说明的目的，并不是用于对本发明的范围进行限制。本领域的技术人员在不背离本发明的宗旨和精神的情况下，可以对本发明进行各种修改和替换。
下述实施例中所使用的实验方法如无特殊说明，均为常规方法。下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。
实施例1短乳杆菌Lactobacillus brevis 8-2B的分离与鉴定
细菌分离自北京波龙堡酒庄，地理区域为北纬39.6°，东经115.9°。酿酒葡萄品种：赤霞珠(Cabernet Sauvignon)。在葡萄成熟的季节,用70％的乙醇消毒塑料袋套住采摘筐，在葡萄园远离两边定植行，选取单数行采摘葡萄，挑选生长势好无病害的葡萄，采摘后快速运往实验室进行后续实验。每一串上不同位置用剪刀剪下完整的葡萄50粒放置在250mL的无菌三角瓶中，加入100mL无菌水，180rpm震荡1h，获得菌悬液，将菌悬液稀释至10-5cfu/mL，吸取100uL稀释液涂布在PDA平板上，28℃培养24h。挑取各平板上形态或颜色不同的菌落到5mL的YPD培养基中并编号，28℃培养24h后。采用平板对峙法对分离的微生物进行拮抗炭黑曲霉筛选。取5uL浓度为107cfu/mL的炭黑曲霉(Aspergillus carbonarius ITEM 5010，该菌种购自意大利菌种保藏中心I.S.P.A.，http://server.ispa.cnr.it/ITEM/Collection/)接种到PDA平板中央，接种5uL筛选菌株于离炭黑曲霉2.0cm处，不接种菌株的为对照组，28℃培养5d观察其生长情况，用游标卡尺测量炭黑曲霉生长直径，计算其抑菌率。最终成功获得能够高效抑制炭黑曲霉的菌株，挑取该菌株的单菌落于LB液体培养基中，培养至对数期，用50％的甘油与培养物等体积混合后置于-80℃保存。
采用16S rDNA序列分析、生理生化等分类法对微生物进行鉴定，结果表明该菌株的分类地位归属于乳杆菌属，短乳杆菌种(Lactobacillus brevis)。该菌株8-2B保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为：CGMCC No.20330。形态特征：该菌株于LB琼脂培养基中，28℃培养24h，其显微形态：杆状，革兰氏染色为阳性，好氧，氧化酶和接触酶为阴性。
生理生化特征：能利用的碳源有L-阿拉伯糖、D-核糖、D-木糖、L-木糖、β-甲基-D木糖甙、D-葡萄糖、D-果糖、α-甲基-D-甘露糖甙、N-乙酰-葡糖胺、七叶灵、麦芽糖、乳糖、蜜二糖、蔗糖、葡萄糖酸盐、2-酮基-葡萄糖酸盐；不能利用的碳源有甘油、赤癣醇、D-阿拉伯糖、阿冬醇、D-半乳糖、D-甘露糖、L-山梨糖、L-鼠李糖、卫茅醇、肌醇、甘露醇、山梨醇、α-甲基-D甘露糖甙、苦杏仁甙、熊果甙、水杨苷、纤维二糖、海藻糖、菊糖、松三糖、棉子糖、淀粉、糖原、木糖醇、龙胆二糖、D-松二糖、D-来苏糖、D-塔格糖、D-岩藻糖、L-岩藻糖、D-阿拉伯糖醇、L-阿拉伯糖醇。
分子生物学鉴定：16S rRNA序列分析：利用引物进行PCR扩增，长度为1461bp的16SrDNA序列(如SEQ ID NO.1所示)与Genbank数据库中已有序列进行Blast比对，该序列与乳杆菌属短乳杆菌种16S rDNA相似度较高，系统发育树如图1所示。
实施例2短乳杆菌Lactobacillus brevis 8-2B对炭黑曲霉生长和产毒的影响
稀释实施例1筛选得到的短乳杆菌8-2B的浓度到104、105、106、107cfu/mL，取100μL涂布到PDA培养基，对照组(CK)为100μL的无菌水。取到107cfu/mL的炭黑曲霉孢子液5μL接种到已接种短杆菌的PDA固体培养基正中央，28℃黑暗条件下培养，采用十字交叉法每天测量炭黑曲霉菌落直径计算抑菌率，培养14d后，每个培养皿中加入10mL的100％甲醇，提取OTA毒素，HPLC检测炭黑曲霉OTA产量。短乳杆菌8-2B抑制炭黑曲霉生长和产毒情况分别见图2、表1。每组数值均代表3个重复的平均值。
如图2所示(图中8-2B代表实施例1筛选得到的短乳杆菌8-2B)，该短乳杆菌8-2B对炭黑曲霉抑制效果明显，尤其是107cfu/mL的短乳杆菌8-2B在13天对炭黑曲霉的抑制率达86％，炭黑曲霉抑制率(％)＝100％×(对照组炭黑曲霉直径-实验组炭黑曲霉直径)/对照组炭黑曲霉直径。如表1所示，该短乳杆菌8-2B也可显著抑制炭黑曲霉产生OTA毒素情况。当在PDA平板上涂布107cfu/mL的短乳杆菌8-2B时，对抑制炭黑曲霉产生OTA毒素的抑制率达97％。炭黑曲霉产生OTA毒素抑制率(％)＝100％×(对照组OTA含量-实验组OTA含量)/对照组OTA含量。
表1短乳杆菌8-2B抑制炭黑曲霉产OTA毒素情况
| 组别 | OTA含量(μg/kg) |
| CK | 265.60±18.87 |
| 10<sup>4</sup> | 166.85±6.16 |
| 10<sup>5</sup> | 80.48±13.91 |
| 10<sup>6</sup> | 25.29±4.26 |
| 10<sup>7</sup> | 8.59±1.66 |
实施例3短乳杆菌Lactobacillus brevis 8-2B对炭黑曲霉孢子萌发和芽管长度的影响
实施例1筛选得到的短乳杆菌8-2B在MRS液体培养基中28℃培养48h，离心去上清，沉淀用无菌PBS清洗2次后配置成104、105、106和107cells/mL的菌悬浮液待用。将炭黑曲霉接种到PDA培养基上，于28℃的恒温培养箱中培养7d。用无菌水(含0.1％的Tween80)冲洗培养皿表面上菌丝体，然后用无菌的纱布过滤，采用血球记数法，孢子浓度调至107cfu/mL。取1mL不同浓度的菌悬液(104、105、106和107cfu/mL)加入到20mL MRS液体培养基中，同时加入1mL 107cfu/mL的炭黑曲霉孢子悬浮液。28℃，180rmp培养12h。滴一滴处理后的菌悬液到载玻片上，盖上盖玻片。光学显微镜下辅以血球计数板，观察并统计各处理中炭黑曲霉孢子萌发率。每个处理最少观察100个孢子，每个处理重复3次，整个试验重复2次。光学显微镜下观察和测量芽管长度并拍照(每个处理选取8个芽管进行测量)。短乳杆菌8-2B抑制炭黑曲霉芽管长度情况如图3所示，短乳杆菌8-2B抑制炭黑曲霉孢子萌发情况如图4所示。图3中CK为对照组(以无菌水替代短乳杆菌的菌悬液)，A、B、C、D分别所对应的短乳杆菌8-2B菌浓度为104、105、106和107cells/mL。图4中CK为对照组(以无菌水替代短乳杆菌的菌悬液)，A、B、C、D分别所对应的短乳杆菌8-2B菌浓度为104、105、106和107cells/mL。107cells/mL短乳杆菌8-2B对炭黑曲霉芽管长度的抑制达73.81％(参见图3)，对炭黑曲霉孢子萌发的抑制率59.89％(参见图4)。
实施例4扫描电镜和透射电镜观察短乳杆菌Lactobacillus brevis 8-2B对炭黑曲霉菌丝的影响
取100μL实施例3中制备得到的1x107cells/mL短乳杆菌8-2B菌悬液涂布到PDA培养基上(作为实验组)，对照组为相同量的无菌水，在培养基正中心接种5μL孢子浓度为107cfu/mL的炭黑曲霉，28℃暗黑条件下培养5d。分别切实验组和对照组的炭黑曲霉菌丝块，用2.5％的戊二醛溶液在4℃下固定24h，用PBS溶液洗涤3次，每次15min；然后用蒸馏水、30％、50％、70％、85％、90％的乙醇水溶液各脱水一次，每次15min；最后用无水乙醇脱水两次，每次20min；脱水完毕后将菌丝块置于载玻片上，CO2临界点干燥仪中干燥、离子溅射仪喷金后置于电镜下观察分析，扫描电压为15kV。扫描电镜结果如图5所示，左图为对照组，右图为实验组，从中可知，短乳杆菌8-2B与炭黑曲霉共培养后，炭黑曲霉菌丝形态发生明显变化，菌丝膨胀，菌丝体断裂成小片。
取100μL实施例3中制备得到的1x107cells/mL短乳杆菌8-2B菌悬液涂布到PDA培养基上(作为实验组)，对照组为相同量的无菌水，在培养基正中心接种5μL孢子浓度为107cfu/mL的炭黑曲霉，28℃暗黑条件下培养5d。分别切实验组和对照组的炭黑曲霉菌丝块，用2.5％戊二醛中固定3h，用PBS缓冲液(pH 7.4)洗涤两次，每次30分钟；再用1.0％四氧化锇固定2h，再用PBS缓冲液(pH 7.4)洗涤30min；然后用50％、60％、70％、80％、90％、95％和100％乙醇溶液依次脱水15min，然后分别用丙酮、乙醇(1:2、1:1和2:0)三种不同的溶液各浸泡15min，然后包埋在环氧树脂介质中。用金刚石刀将其切成70nm的超薄切片，切片经醋酸铀酰溶液和柠檬酸铅溶液各染色25min，即可在透射电镜下观察。透射电镜观察结果如图6所示，左图为对照组，右图为实验组，从中可知，短乳杆菌8-2B与炭黑曲霉共培养后，炭黑曲霉细胞壁、细胞膜等结构均有显著改变，细胞壁变薄，胞膜破裂，质壁分离，细胞器坏死。
实施例5短乳杆菌Lactobacillus brevis 8-2B在葡萄表皮对炭黑曲霉生长的影响
实施例1筛选得到的短乳杆菌8-2B在MRS液体培养基中28℃培养48h，8000rpm离心3min，去上清，用无菌水洗沉淀，制成107cells/mL菌悬浮液。炭黑曲霉接种到PDA培养基中，于28℃的恒温培养箱中黑暗培养7d。培养皿中加入10mL无菌水(含0.1％的吐温80)用无菌棉签刮下孢子及菌丝体，然后用无菌的纱布过滤，采用血球记数法，调节孢子浓度为107cfu/mL。挑选大小、成熟度一致的果实，用剪刀将葡萄果粒从叶茎上剪下，每个葡萄果粒上留有0.5cm的叶柄，防止葡萄果粒干枯。将葡萄果粒浸入无菌的1％的无菌次氯酸钠溶液中3min，然后用无菌水冲洗4遍，在无菌操作台中晾干。待葡萄果粒表面无水时，在葡萄果柄处接种100μL短乳杆菌8-2B菌悬浮液(作为实验组)，对照组采用等量无菌水，无菌操作台上培养2h，再接种100μL炭黑曲霉孢子悬浮液。将处理好的葡萄果粒果柄向下放在微孔板中，每个微孔板放置24粒葡萄，然后放入无菌的保鲜盒中，25℃恒温保湿培养箱中培养。对照组和实验组均进行3个重复。每两天记录每个处理的葡萄的腐烂数量，并计算其腐败率。腐败率(％)＝100％×腐败葡萄数量/葡萄总数量。
短乳杆菌8-2B抑制葡萄腐烂情况如图7所示，由图7可知，炭黑曲霉在葡萄皮上生长缓慢，前7天葡萄粒无明显腐败现象，第11天之后对照组葡萄粒腐败数明显增加，第19天腐败率达到62.8％，33天达到85.83％。短乳杆菌8-2B处理的葡萄腐败比较缓慢，前19天一直保持在20％以下，第33天腐败率为30.55％。与对照组相比差异显著。
实施例6短乳杆菌Lactobacillus brevis 8-2B在葡萄体内对炭黑曲霉生长的影响
实施例1筛选得到的短乳杆菌8-2B在MRS液体培养基中28℃培养48h，8000rpm离心3min，去上清，用无菌水洗沉淀，制成107cells/mL菌悬浮液。炭黑曲霉接种到PDA培养基中，于28℃的恒温培养箱中黑暗培养7d。培养皿中加入10mL无菌水(含0.1％的吐温80)用无菌棉签刮下孢子及菌丝体，然后用无菌的纱布过滤，采用血球记数法，调节孢子浓度为107cfu/mL。挑选大小、成熟度一致的果实，用剪刀将葡萄果粒从叶茎上剪下，每个葡萄果粒上留有0.5cm的叶柄，防止葡萄果粒干枯。将葡萄果粒浸入无菌的1％的无菌次氯酸钠溶液中3min，然后用无菌水冲洗4遍，在无菌操作台中晾干。待葡萄果粒表面无水时，在其葡萄顶端用无菌接种针打孔2mm的小孔，将葡萄浸泡在50mL的短乳杆菌8-2B菌悬浮液中3min取出(作为实验组)，对照组浸泡含0.1％吐温80的无菌水，在无菌操作台上培养2h，在打孔部位加入5μL炭黑曲霉孢子悬浮液。将处理好的葡萄果粒果柄向下放在微孔板中，每个微孔板放置24粒葡萄，然后放入无菌的保鲜盒中，加入1mL无菌水，保证保鲜盒的湿度为95％，盖好盖子于25℃恒温保湿培养箱中培养。对照组和实验组均进行3个重复。记录第1d至第15d时每个处理的葡萄的腐烂数量，并计算其腐败率。
图8为短乳杆菌8-2B在葡萄上对炭黑曲霉的影响照片；图8A为对照组，图8B为实验组。图9为短乳杆菌8-2B抑制葡萄腐烂情况统计图。如图8和图9所示，短乳杆菌8-2B对抑制葡萄因炭黑曲霉污染所导致的腐烂效果明显，与对照组比较发现，短乳杆菌8-2B处理的葡萄培养4d的时候，腐败率低于30％，第7d葡萄腐败率达到33.3％，并且保持一个缓慢增加的程度，而对照组的葡萄的随着储藏时间的增加，腐败率快速增加，第7d葡萄腐败率达到95.8％。说明短乳杆菌8-2B对葡萄上炭黑曲霉抑制效果明显。
虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。
序列表
<110> 中国农业科学院农产品加工研究所
<120> 一种短乳杆菌8-2B及其应用
<130> KHP201113867.9
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1461
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
ggcggcatgc ctatacatgc aagtcgaacg agcttccgtt gaatgacgtg cttgcactga 60
tttcaacaat gaagcgagtg gcgaactggt gagtaacacg tgggaaatct gcccagaagc 120
aggggataac acttggaaac aggtgctaat accgtataac aacaaaatcc gcatggattt 180
tgtttgaaag gtggcttcgg ctatcacttc tggatgatcc cgcggcgtat tagttagttg 240
gtgaggtaaa ggcccaccaa gacgatgata cgtagccgac ctgagagggt aatcggccac 300
attgggactg agacacggcc caaactccta cgggaggcag cagtagggaa tcttccacaa 360
tggacgaaag tctgatggag caatgccgcg tgagtgaaga agggtttcgg ctcgtaaaac 420
tctgttgtta aagaagaaca cctttgagag taactgttca agggttgacg gtatttaacc 480
agaaagccac ggctaactac gtgccagcag ccggcggtaa tacgtaggtg gcaagcgttg 540
tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgat gtgaaagcct 600
tcggcttaac cggagaagtg catcggaaac tgggagactt gagtgcagaa gaggacagtg 660
gaactccatg tgtagcggtg gaattgcgta gatatatgga agaacaccag tggcgaaggc 720
ggctgtctag tctgtaactg acgctgaggc tcgaaagcat gggtagcgaa caggattaga 780
taccctggta gtccatgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 840
agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 900
caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagctacg 960
cgaagaacct taccaggtct tgacatcttc tgccaatctt agagataaga cgttcccttc 1020
ggggacagaa tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1080
aagtcccgca acgagcgcaa cccttattat cagttgccag cattcagttg ggcactctgg 1140
tgagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1200
atgacctggg ctacacacgt gctacaatgg acggtacaac gagttgcgaa gtcgtgaggc 1260
taagctaatc tcttaaagcc gttctcagtt cggattgtag gctgcaactc gcctacatga 1320
agttggaatc gctagtaatc gcggatcagc atgccgcggt gaatacgttc ccgggccttg 1380
tacacacgcc cgtcacacca tgagagtttg taacacccaa agccggtgag ataaccttcg 1440
ggagtcagcc gtctaaggtg g 1461
Claims (10)
1.短乳杆菌(Lactobacillus brevis)8-2B，其特征在于，所述短乳杆菌(Lactobacillus brevis)8-2B的保藏编号为CGMCC No.20330。
2.权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B的发酵产物。
3.一种菌剂，其特征在于，含有权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物。
4.权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物或权利要求3所述的菌剂在抑制炭黑曲霉生长中的应用。
5.权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物或权利要求3所述的菌剂在抑制炭黑曲霉生产赭曲霉毒素A中的应用。
6.权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物或权利要求3所述的菌剂在农产品防霉中的应用。
7.根据权利要求6所述的应用，其特征在于，所述农产品为葡萄。
8.一种炭黑曲霉生长抑制剂，其特征在于，含有权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物。
9.一种抑制炭黑曲霉生产赭曲霉毒素A的抑制剂，其特征在于，含有权利要求1所述的短乳杆菌(Lactobacillus brevis)8-2B或权利要求2所述的发酵产物。
10.一种农产品防霉剂，其特征在于，含有权利要求1所述的短乳杆菌(Lactobacillusbrevis)8-2B或权利要求2所述的发酵产物。
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