CN112055716B - 用于可激发和不可激发细胞中Ca2+成像的低亲和力红色荧光指示剂 - Google Patents

用于可激发和不可激发细胞中Ca2+成像的低亲和力红色荧光指示剂 Download PDF

Info

Publication number
CN112055716B
CN112055716B CN201980028715.9A CN201980028715A CN112055716B CN 112055716 B CN112055716 B CN 112055716B CN 201980028715 A CN201980028715 A CN 201980028715A CN 112055716 B CN112055716 B CN 112055716B
Authority
CN
China
Prior art keywords
glu
gly
lys
asp
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980028715.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN112055716A (zh
Inventor
张郁芬
吴嘉辉
M·J.·丹尼尔斯
R·E.·坎贝尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Alberta
University of Oxford
Original Assignee
University of Alberta
University of Oxford
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Alberta, University of Oxford filed Critical University of Alberta
Publication of CN112055716A publication Critical patent/CN112055716A/zh
Application granted granted Critical
Publication of CN112055716B publication Critical patent/CN112055716B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201980028715.9A 2018-03-02 2019-03-01 用于可激发和不可激发细胞中Ca2+成像的低亲和力红色荧光指示剂 Active CN112055716B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862637808P 2018-03-02 2018-03-02
US62/637,808 2018-03-02
PCT/CA2019/050254 WO2019165563A1 (en) 2018-03-02 2019-03-01 Low affinity red fluorescent indicators for imaging ca2+ in excitable and non-excitable cells

Publications (2)

Publication Number Publication Date
CN112055716A CN112055716A (zh) 2020-12-08
CN112055716B true CN112055716B (zh) 2024-11-01

Family

ID=67804835

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980028715.9A Active CN112055716B (zh) 2018-03-02 2019-03-01 用于可激发和不可激发细胞中Ca2+成像的低亲和力红色荧光指示剂

Country Status (8)

Country Link
US (1) US20210063404A1 (https=)
EP (1) EP3759128A4 (https=)
JP (1) JP7313636B2 (https=)
KR (1) KR102895185B1 (https=)
CN (1) CN112055716B (https=)
CA (1) CA3093019A1 (https=)
SG (1) SG11202007933VA (https=)
WO (1) WO2019165563A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115197301B (zh) * 2022-06-10 2024-07-16 北京师范大学 用于胞内钙信号检测及相关药物筛选的钙指示工具及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1700865A1 (en) * 2005-03-11 2006-09-13 AXXAM S.r.l. Photoproteins with enhanced bioluminescence and their use as intracellular calcium indicators
US20110154515A1 (en) * 2007-08-24 2011-06-23 Oliver Griesbeck Genetically encoded calcium sensors comprising the c-terminal lobe of troponin c and a fluorescence tag
JP6051438B2 (ja) * 2012-06-19 2016-12-27 国立大学法人埼玉大学 赤色蛍光蛋白質を用いたカルシウムセンサー蛋白質
US10836802B2 (en) * 2014-06-11 2020-11-17 Japan Science And Technology Agency Calcium reporter gene
US9644007B2 (en) * 2014-12-23 2017-05-09 Howard Hughes Medical Institute Red genetically encoded calcium indicators and methods of use
CA2997619A1 (en) * 2015-09-15 2017-03-23 The Board Of Trustees Of The Leland Stanford Junior University Light-responsive polypeptides and methods of use thereof
WO2017087542A1 (en) * 2015-11-18 2017-05-26 The Board Of Trustees Of The Leland Stanford Junior University Method and systems for measuring neural activity
CN205757212U (zh) * 2016-06-29 2016-12-07 湖南中烟工业有限责任公司 无棉型超声波雾化器及电子烟
WO2018034298A1 (ja) * 2016-08-16 2018-02-22 国立研究開発法人理化学研究所 カルシウムインジケーターを有する遺伝子改変トリパノソーマ・クルージおよび抗トリパノソーマ薬のスクリーニングのためのその応用
EP3652339A4 (en) * 2017-07-09 2021-04-14 Montana Molecular LLC BIOSENSORS FOR MEASURING CELL SIGNALING IN STRESSED AND HEALTHY CELLS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jiahui Wu.Development of red fluorescent protein-based calcium ion and glutamate indicators.2014,第三章和第四章. *

Also Published As

Publication number Publication date
EP3759128A1 (en) 2021-01-06
SG11202007933VA (en) 2020-09-29
JP2021515550A (ja) 2021-06-24
KR102895185B1 (ko) 2025-12-03
CN112055716A (zh) 2020-12-08
WO2019165563A9 (en) 2020-09-10
WO2019165563A1 (en) 2019-09-06
CA3093019A1 (en) 2019-09-06
EP3759128A4 (en) 2021-12-08
KR20210002467A (ko) 2021-01-08
JP7313636B2 (ja) 2023-07-25
US20210063404A1 (en) 2021-03-04

Similar Documents

Publication Publication Date Title
Chu et al. A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions
CN113501881A (zh) 融合蛋白
WO2019062744A1 (zh) 融合多肽
US9365623B2 (en) Cyanochrome fluorophores
CN112055716B (zh) 用于可激发和不可激发细胞中Ca2+成像的低亲和力红色荧光指示剂
Peroza et al. Rapid, randomized development of genetically encoded FRET sensors for small molecules
CA2384170C (en) Cgmp-visualizing probe and method of detecting and quantifying cgmp by using the same
JP2022529592A (ja) 分裂した光活動性黄色タンパク質の相補体化系およびその使用
US12601016B2 (en) Genetically encoded fluorescent indicators under optogenetic control and uses thereof
Luptak et al. Genetically targeted fluorescent probes reveal dynamic calcium responses to adrenergic signaling in multiple cardiomyocyte compartments
US20260072030A1 (en) Ras biosensors
US20240369485A1 (en) Fluorescent sensor for monitoring calcium dynamics
HK40112057A (zh) 用於监测钙动力学的荧光传感器
US12098184B2 (en) G protein-coupled receptor (GPCR) ligand assay
WO2024216202A2 (en) Fret biosensor recombinant proteins
Takaramoto et al. Significance of extended N-terminal region and a first motif residue in a third transmembrane helix of a novel pump-like cation channelrhodopsin HulaCCR
He et al. Highly Sensitive Chemigenetic FRET-Based Kinase Biosensors
ES2318151T3 (es) Procedimiento de seleccion de agentes bio-activos por deteccion de una variacion inducida de la concentracion intracelular de ampc de una celula viviente sensible.
Ford Design and implementation of transgenic tools to visualise cell cycle progression in mammalian development
Wu Development of red fluorescent protein-based calcium ion and glutamate indicators
Marchetti Dynamics and interactions of an oncogenic homeotic protein within human replicative complexes
Dosunmu-Ogunbi et al. Structure of a highly conserved domain of rock1 required for shroom-mediated regulation of cell morphology
Greotti Development of new tools to explore organelle calcium dynamics in vivo: a new fret-based calcium sensor and a mitochondria targeted channelrhodopsin
Ganesan Local Protein Turnover As a Regulatory Mechanism of Growth and Collapse of Neuronal Growth Cones
Cardarelli Nanostructuring HIV-1 Tat arginine-rich motif for targeted cellular delivery

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant