CN112041431A - 产生t细胞谱系细胞的方法 - Google Patents

产生t细胞谱系细胞的方法 Download PDF

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CN112041431A
CN112041431A CN201980025370.1A CN201980025370A CN112041431A CN 112041431 A CN112041431 A CN 112041431A CN 201980025370 A CN201980025370 A CN 201980025370A CN 112041431 A CN112041431 A CN 112041431A
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cells
cell
stem
notch
progenitor
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J·C·阻尼加-普鲁科尔
M·莫塔希亚米
A·特罗特曼-格兰特
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Sunnybrook Research Institute
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
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    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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CN201980025370.1A 2018-02-14 2019-02-14 产生t细胞谱系细胞的方法 Pending CN112041431A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862630497P 2018-02-14 2018-02-14
US62/630,497 2018-02-14
PCT/CA2019/050181 WO2019157597A1 (en) 2018-02-14 2019-02-14 Method for generating cells of the t cell lineage

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CN112041431A true CN112041431A (zh) 2020-12-04

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US (2) US12467034B2 (https=)
EP (1) EP3752602A4 (https=)
JP (3) JP2021514185A (https=)
KR (1) KR20200121817A (https=)
CN (1) CN112041431A (https=)
AU (2) AU2019220935B2 (https=)
CA (1) CA3091158A1 (https=)
WO (1) WO2019157597A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12467034B2 (en) 2018-02-14 2025-11-11 Sunnybrook Research Institute Method for generating cells of the T cell lineage

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023548115A (ja) * 2020-10-28 2023-11-15 サンガモ セラピューティクス， インコーポレイテッド ヒト多能性幹細胞からのｃｄ４＋エフェクターおよび制御性ｔ細胞の生成
WO2022115492A1 (en) * 2020-11-24 2022-06-02 Lyell Immunopharma, Inc. Methods for making, compositions comprising, and methods of using rejuvenated t cells
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
AU2021392032A1 (en) 2020-12-03 2023-06-22 Century Therapeutics, Inc. Genetically engineered cells and uses thereof
KR20230128324A (ko) * 2021-02-05 2023-09-04 고쿠리츠다이가쿠호진 고베다이가쿠 인공 다능성 줄기 세포 유래 γδ T 세포 및 그 제작방법
WO2022216524A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Combined artificial cell death/reporter system polypeptide for chimeric antigen receptor cell and uses thereof
CA3210702A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Gene transfer vectors and methods of engineering cells
WO2022241558A1 (en) * 2021-05-18 2022-11-24 The University Of British Columbia A method for producing blood progenitor and progenitor t cells, resulting cells and methods and uses thereof
WO2023129937A1 (en) 2021-12-29 2023-07-06 Century Therapeutics, Inc. Genetically engineered cells having anti-cd19 / anti-cd22 chimeric antigen receptors, and uses thereof
IL314801A (en) 2022-02-22 2024-10-01 Juno Therapeutics Inc Proteinase 3 (PR3) Chimeric Autoantigen Receptor T Cells and Related Methods and Uses
CN119365492A (zh) 2022-05-04 2025-01-24 世纪治疗股份有限公司 用hla-e和hla-g转基因工程化的细胞
JP2025522360A (ja) 2022-06-08 2025-07-15 センチュリー セラピューティクス，インコーポレイテッド Ｃｄ１６変異体およびｎｋｇ２ｄを発現する遺伝子操作した細胞ならびにその使用
CA3257650A1 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Immunoeffector cells derived from genetically modified, multipotential stem cells with membrane IL-12 and their uses
WO2023240212A2 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically engineered cells having anti-cd133 / anti-egfr chimeric antigen receptors, and uses thereof
WO2024102838A1 (en) 2022-11-09 2024-05-16 Century Therapeutics, Inc. Engineered interleukin-7 receptors and uses thereof
KR20250143301A (ko) 2022-11-10 2025-10-01 센츄리 쎄라퓨틱스 인코포레이티드 항-넥틴4 키메라 항원 수용체를 갖는 유전자 조작 세포 및 이의 용도
WO2024156066A1 (en) * 2023-01-27 2024-08-02 Notch Therapeutics (Canada), Inc. Method for generating t cell lineage populations from stem/progenitor cells
WO2025101938A2 (en) 2023-11-10 2025-05-15 Century Therapeutics, Inc. Genetically engineered cells having multi-transmembrane domain chimeric antigen receptors utilizing g protein-coupled receptor scaffolds, and uses thereof
WO2025106626A1 (en) 2023-11-15 2025-05-22 Century Therapeutics, Inc. Genetically engineered cells expressing c-x-c chemokine receptor type 4, and uses thereof
WO2025207798A1 (en) 2024-03-26 2025-10-02 Century Therapeutics, Inc. Genetically engineered cells having anti-cd123 chimeric antigen receptors, and uses thereof
WO2025207795A1 (en) 2024-03-26 2025-10-02 Juno Therapeutics, Inc. Genetically engineered cells having anti-cd33 / anti-cd123 chimeric antigen receptors, and uses thereof
US20250332260A1 (en) 2024-04-10 2025-10-30 Garuda Therapeutics, Inc. Immune compatible cells for allogeneic cell therapies to cover global, ethnic, or disease-specific populations

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087159A2 (en) * 2002-04-05 2003-10-23 Lorantis Limited Modulators of the notch signalling pathway and uses thereof in medical treatment
US20040171148A1 (en) * 2002-12-10 2004-09-02 Schmitt Thomas M. Cell preparations comprising cells of the T cell lineage and methods of making and using them
JP2006517533A (ja) * 2003-01-23 2006-07-27 ロランティス リミテッド Ｎｏｔｃｈシグナル伝達経路のアクチベーターを用いる自己免疫疾患の治療
WO2007027226A2 (en) * 2005-04-28 2007-03-08 Board Of Regents, The University Of Texas System Systems and methods for the production of differentiated cells
US8772028B2 (en) * 2008-11-07 2014-07-08 Sunnybrook Health Sciences Centre CD34+CD7+CD5+CD1a-human progenitor T-cells produced in vitro and methods of using
CN104053769A (zh) * 2011-11-21 2014-09-17 大学健康网络 造血祖细胞群体和富集其干细胞的方法
WO2016055396A1 (fr) * 2014-10-06 2016-04-14 Assistance Publique - Hopitaux De Paris Méthode de génération de progéniteurs de cellules t
WO2017173551A1 (en) * 2016-04-08 2017-10-12 The Governing Council Of The University Of Toronto Method for generating progenitor t cells from stem and/or progenitor cells and use of same
CN107429230A (zh) * 2015-01-26 2017-12-01 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
WO2022221962A1 (en) * 2021-04-23 2022-10-27 Sunnybrook Research Institute Stem cells comprising an unrearranged t cell receptor (tcr) gene locus and methods of use thereof
WO2022241558A1 (en) * 2021-05-18 2022-11-24 The University Of British Columbia A method for producing blood progenitor and progenitor t cells, resulting cells and methods and uses thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100895231B1 (ko) 2002-03-25 2009-05-04 다카라 바이오 가부시키가이샤 세포상해성 림프구의 제조방법
EP2321407A4 (en) 2008-09-11 2012-07-18 Univ Florida SYSTEM AND METHOD FOR THE PRODUCTION OF T-CELLS
US8871510B2 (en) 2009-12-03 2014-10-28 University Of Utah Research Foundation Methods for generating T lymphocytes from hematopoietic stem cells
ES2608937T3 (es) 2010-02-22 2017-04-17 Université Pierre Et Marie Curie (Paris 6) Medio de cultivo celular para el crecimiento y diferenciación de células del linaje hematopoyético
US10180254B2 (en) * 2014-09-16 2019-01-15 Hitachi Zosen Inova Ag Method and device for processing slag occurring in a combustion chamber of a refuse incineration plant
WO2016187459A1 (en) 2015-05-20 2016-11-24 The Regents Of The University Of California Method for generating human dendritic cells for immunotherapy
US20190134169A1 (en) 2016-04-26 2019-05-09 Temple University-Of The Commonwealth System Of Higher Education DLL4-expressing cells and vaccine using the same
IL268641B2 (en) 2017-02-13 2024-10-01 Assist Publique Hopitaux Paris Aphp Method for generating t cells progenitors
JP2021514185A (ja) 2018-02-14 2021-06-10 サニーブルック リサーチ インスティチュート Ｔ細胞系列の細胞を発生させるための方法

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087159A2 (en) * 2002-04-05 2003-10-23 Lorantis Limited Modulators of the notch signalling pathway and uses thereof in medical treatment
US20040171148A1 (en) * 2002-12-10 2004-09-02 Schmitt Thomas M. Cell preparations comprising cells of the T cell lineage and methods of making and using them
JP2006517533A (ja) * 2003-01-23 2006-07-27 ロランティス リミテッド Ｎｏｔｃｈシグナル伝達経路のアクチベーターを用いる自己免疫疾患の治療
WO2007027226A2 (en) * 2005-04-28 2007-03-08 Board Of Regents, The University Of Texas System Systems and methods for the production of differentiated cells
US8772028B2 (en) * 2008-11-07 2014-07-08 Sunnybrook Health Sciences Centre CD34+CD7+CD5+CD1a-human progenitor T-cells produced in vitro and methods of using
CN104053769A (zh) * 2011-11-21 2014-09-17 大学健康网络 造血祖细胞群体和富集其干细胞的方法
WO2016055396A1 (fr) * 2014-10-06 2016-04-14 Assistance Publique - Hopitaux De Paris Méthode de génération de progéniteurs de cellules t
CN107429230A (zh) * 2015-01-26 2017-12-01 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
WO2017173551A1 (en) * 2016-04-08 2017-10-12 The Governing Council Of The University Of Toronto Method for generating progenitor t cells from stem and/or progenitor cells and use of same
CN109312307A (zh) * 2016-04-08 2019-02-05 多伦多大学管理委员会 用于从干细胞和/或祖细胞生成祖t细胞的方法及其用途
WO2022221962A1 (en) * 2021-04-23 2022-10-27 Sunnybrook Research Institute Stem cells comprising an unrearranged t cell receptor (tcr) gene locus and methods of use thereof
WO2022241558A1 (en) * 2021-05-18 2022-11-24 The University Of British Columbia A method for producing blood progenitor and progenitor t cells, resulting cells and methods and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ASHTON C TROTMAN-GRANT等: "DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system", NAT COMMUN, vol. 12, pages 1 - 11 *
JASTARANPREET SINGH等: "Producing proT cells to promote immunotherapies", INTERNATIONAL IMMUNOLOGY, vol. 30, pages 541 *
LEDI LIU: "Identification of domains for efficient notch signaling activity in immobilized notch ligand proteins", J CELL BIOCHEM, vol. 118, pages 786 *
NADINE GEHRE等: "A stromal cell free culture system generates mouse pro-T cells that can reconstitute T-cell compartments in vivo", EUR J IMMUNOL, vol. 45, pages 932 - 942, XP055392254, DOI: 10.1002/eji.201444681 *
SABIA TAQVI等: "Biomaterial-based botch signaling for the differentiation of hematopoietic stem cells into T cells", J BIOMED MATER RES A, vol. 79, pages 689 - 697 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12467034B2 (en) 2018-02-14 2025-11-11 Sunnybrook Research Institute Method for generating cells of the T cell lineage

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AU2019220935B2 (en) 2025-03-20
AU2025204516A1 (en) 2025-07-03
JP2021514185A (ja) 2021-06-10
US20200399599A1 (en) 2020-12-24
US12467034B2 (en) 2025-11-11
EP3752602A4 (en) 2021-11-24
JP2026031647A (ja) 2026-02-24
AU2019220935A1 (en) 2020-10-01
JP2024016217A (ja) 2024-02-06
WO2019157597A1 (en) 2019-08-22
US20260043003A1 (en) 2026-02-12
EP3752602A1 (en) 2020-12-23
KR20200121817A (ko) 2020-10-26
CA3091158A1 (en) 2019-08-22

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